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  • vitro
  • However, because NS cells arise in vitro from heterogeneous populations their immediate cellular origin remains unclear. (biomedsearch.com)
  • We speculate that the self-renewing NS cell state may be generated in vitro following transcriptional resetting induced by FGF. (biomedsearch.com)
  • The effects of the age of cell donor animal on in vitro development of ovine nuclear transfer (NT) embryos were investigated. (biomedsearch.com)
  • It is widely anticipated that human embryonic stem (ES) cells will serve as an experimental model for studying early development in our species, and, conversely, that studies of development in model systems, the mouse in particular, will inform our efforts to manipulate human stem cells in vitro. (biologists.org)
  • Conversely, will our understanding of the molecular regulation of mammalian development based on studies in the mouse provide us with a framework to control hES cells in vitro, enabling us to develop the many important clinical applications that are envisioned for these cells in the future? (biologists.org)
  • The mouse F9 EC cell line and a tumor-bearing mouse model were used to evaluate the anti-EC tumor effects of TCF3 in vitro and in vivo, respectively. (spandidos-publications.com)
  • These results suggest that the isolation of ES cells depends upon reversible, epigenetic changes in the pattern of gene expression and that the heat shock and puromycin treatments utilised here, may have helped to promote these changes in vitro . (bl.uk)
  • Platelet derived growth factors have been shown to stimulate cell proliferation efficiently in vivo 1,2 and in vitro . (jove.com)
  • These characteristics make neural stem and progenitor cells an invaluable renewable source of cells for in vitro studies such as drug screening, neurotoxicology and electrophysiology and also for cell replacement therapy in many neurological diseases. (jove.com)
  • These unique characteristics lead to the exciting prospect of using these cells to study disease processes and developmental pathways in vitro and eventually to treat a wide variety of diseases using cell replacement therapies. (upenn.edu)
  • My research program focuses on understanding the molecular mechanisms that regulate endoderm and mesoderm development utilizing the in vitro differentiation of ES cells and iPS cells. (upenn.edu)
  • We are studying the molecular pathways which regulate megakaryopoeisis with the goal of optimizing the generation of platelets in vitro from ES/iPS cells. (upenn.edu)
  • In addition, we are developing in vitro models of platelet disorders using iPS cells derived from patients with genetic diseases affecting platelet development and function as well as using CRISPR/Cas9 technology to both correct mutant iPS cell lines and generate patient specific mutations in wild-type lines. (upenn.edu)
  • A human hepatoma cell line (Huh-7 based) was used for transfection of in vitro transcribed HCV genomic RNAs. (jove.com)
  • HCV in vitro research is therefore limited to human hepatoma cell lines permissive for virus entry and completion of the viral life cycle. (jove.com)
  • However, there is currently no in vitro assay to measure the impact of such blockade on cytokine secretion in cells from human samples. (jove.com)
  • Here, we depict an in vitro experimental design that enables measurements of cytokine secretion by HIV-specific CD4 T cells and their impact on other cell subsets. (jove.com)
  • In vitro cell experiments are used to specifically evaluate cell type-dependent changes caused by a substance of interest, but culture-dependent variations and the inability to analyze the influence of specific molecules in the context of the inflammatory compound in atherosclerotic lesions limit the impact of the results. (jove.com)
  • To do so, researchers take advantage for two light-producing luciferase enzymes isolated from the firefly ( Photinus pyralis ) and sea pansy ( Renilla reniformis ), both of which react to mutually exclusive substrates that previously facilitated their wide-spread use in in vitro cell-based reporter gene assays 4 . (jove.com)
  • More importantly, residual transgene expression in undifferentiated human iPSCs could hamper proper differentiations and misguide the interpretation of disease-relevant in vitro phenotypes. (jove.com)
  • In in vitro analysis induced cells showed slow proliferation and were sensitized to differentiation-inducing treatment, and in vivo tumorigenesis was reduced in NOD/SCID mice. (pnas.org)
  • genetic
  • Here, we conducted genetic studies to investigate the role of Utf1 (Undifferentiated embryonic cell transcription factor 1) in mouse germ cell development. (readbyqxmd.com)
  • Exploring the genetic, molecular and biological features of neural differentiation process from embryonic and patient-specific stem cells may offer an unprecedented chance to understand normal and abnormal human brain development and diseases, with implications for drug development and potential cellular replacement therapy. (yale.edu)
  • We are using a multifaceted approach with investigators of the Yale Stem Cell Center, integrating genomic, proteomic, and genetic experiments to elucidate the molecular events that control neural cell differentiation using hES cells as model system. (yale.edu)
  • My current efforts are focused on a novel type of unbiased, genome-wide functional screen to identify genetic mechanisms that control the steps of the neural differentiation in hES cells. (yale.edu)
  • Techniques for the isolation and genetic manipulation of embryonic stem cells (ES cells) in the mouse are well established. (bl.uk)
  • Lastly, we are using the stem cell system to model genetic forms of diabetes, using both patient specific iPS cells and genome editing to generate genetic models of these diseases. (upenn.edu)
  • Inefficient replication of HCV in non-human cells e.g. of mouse origin is likely due to lack of genetic incompatibility of essential host dependency factors and/or expression of restriction factors. (jove.com)
  • the genetic modification may be transmitted to successive generations if the ES cells contribute to the germline. (sigmaaldrich.com)
  • SAN FRANCISCO, CA -March 6, 2008--Researchers at the Gladstone Institute of Cardiovascular Disease (GICD) and the University of California, San Francisco have identified for the first time how tiny genetic factors called microRNAs may influence the differentiation of pluripotent embryonic stem (ES) cells into cardiac muscle. (rutgers.edu)
  • Potential uses of human iPSCs include modeling pathogenesis of human genetic diseases, autologous cell therapy after gene correction, and personalized drug screening by providing a source of patient-specific and symptom relevant cells. (jove.com)
  • regulate
  • These results suggest that PPARγ agonists regulate LIF signaling through JAK-STAT pathway leading to growth and self-renewal of ES cells. (ovid.com)
  • Follicular dermal cells possess properties that regulate induction of follicle formation, hair growth, and follicle end bulb regeneration after amputation. (dur.ac.uk)
  • mesoderm
  • The following cell types were identified by examination of the histological sections of 14-day-old EBs stained with H&E: (A) neural rosette (ectoderm), (B) neural rosette stained with Tubulin, (C) primitive blood vessels (mesoderm), and (D) megakaryocytes (mesoderm). (bioind.com)
  • Specifically, they found that miR-1 and miR-133 are active at the early stages of heart cell formation, when an ES cell is first "deciding" to become mesoderm, one of the three basic tissue layers in mammals and other organisms. (rutgers.edu)
  • transcriptome
  • Transcriptome mapping analysis has determined the chromosomal domains of gene coexpression patterns specific to the ES state and demonstrated that regulation of ES cell development is operative at both the local chromosomal domain level and global level. (begellhouse.com)
  • stages
  • How useful a tool will these cells be for studying these developmental stages? (biologists.org)
  • ES cells isolated from the three different embryonic stages equivalent to three days of development, have the same morphological characteristics. (bl.uk)
  • An HCV cell culture system is critical for studying various stages of HCV growth including viral entry, genome replication, packaging, and egress. (jove.com)
  • With the investigation and development of ES cells from zygote to blastodermic vesicle stages, the elucidation of the molecular mechanisms that specify pluripotent differentiation has made remarkable progress ( 4 , 5 ). (pnas.org)
  • molecular
  • sation of the follicle, together with its role as a stem cell repository, make interpretations of molecular function within the follicle, complex. (dur.ac.uk)
  • Murine
  • The objective of this thesis was to investigate various factors which may influence the potential of murine and ovine embryos to yield ES cells. (bl.uk)
  • Although HCV RNA replication has been observed in human non-hepatic and murine cell lines, the efficiency was very low and required long-term selection procedures using HCV replicon constructs expressing dominant antibiotic-selectable markers 1-5 . (jove.com)
  • Here, a preclinical, murine pancreatic tumor model of hepatic metastases via a hemispleen injection of syngeneic murine pancreatic tumor cells is described. (jove.com)
  • regulation
  • First, we will describe the steps necessary to construct dual bioluminescent reporter cell lines, as well as those needed to facilitate their use in visualizing the spatiotemporal regulation of gene expression during specific steps of the metastatic cascade. (jove.com)
  • human
  • Our observations attest to the concept that in metazoan models of aging most pathology occurs in organs made up of slowly dividing or non-dividing cells and that impaired DNA repair and genomic instability, driven by oxidative damage, may underlie normal human aging. (yale.edu)
  • Creation of disease models, both in human cell culture and animals, is crucial. (yale.edu)
  • Human adult adipose tissue is an abundant source of mesenchymal stem cells (MSCs). (jove.com)
  • Recently, using fluorescence-activated cell sorting (FACS), we and other researchers prospectively identified and purified three subpopulations of multipotent precursor cells associated with the vasculature of human skeletal muscle. (jove.com)
  • All of these human blood-vessel-derived stem cell (hBVSC) populations not only express classic MSC markers but also possess mesodermal developmental potentials similar to typical MSCs. (jove.com)
  • Proinflammatory factors from activated T cells inhibit neurogenesis in adult animal brain and cultured human fetal neural stem cells (NSC). (jove.com)
  • We generated NSC from adult human peripheral CD34+ cells by transfecting the cells with Sendai virus constructs containing Sox2, Oct3/4, c-Myc and Klf4. (jove.com)
  • We also have a focus on pancreatic beta cell specification with the goal of generating functional beta cells from human ES and iPS cells. (upenn.edu)
  • GATA6 suppression enhances lung specification from human pluripotent stem cells. (upenn.edu)
  • Identifying and enriching the platelet-producing human stem cell-derived megakaryocytes using factor V uptake. (upenn.edu)
  • We investigated whether HCV propagation is suppressed by dominant restriction factors in either human cell lines derived from non-hepatic tissues or in mouse liver cell lines. (jove.com)
  • Specifically, subgenomic HCV replicons carrying a luciferase transgene were transfected into highly permissive human hepatoma cells (Huh-7.5 cells). (jove.com)
  • NutriStem® hPSC XF Medium is a widely published, defined, xeno-free, serum-free cell culture medium designed to support the growth and expansion of human induced pluripotent stem (hiPS) and human embryonic stem (hES) cells. (bioind.com)
  • Figure: 1 Human Embryonic Stem Cells (H1, passage 6) were seeded in 96-well plates (Matrigel coated) in BI's NutriStem® hPSC XF and competitor's media. (bioind.com)
  • This manuscript illustrates a protocol for efficiently creating integration-free human induced pluripotent stem cells (iPSCs) from peripheral blood using episomal plasmids and histone deacetylase (HDAC) inhibitors. (jove.com)
  • Once iPSC colonies exhibit typical human embryonic stem cell (hESC) morphology, they are gently transferred to individual iMEF-coated tissue culture plates for continued growth and expansion. (jove.com)
  • A few years ago, the establishment of human induced pluripotent stem cells (iPSCs) ushered in a new era in biomedicine. (jove.com)
  • epigenetic
  • DNA methylation regulates development and many epigenetic processes in mammals, and it is required for somatic cell growth and survival. (biomedsearch.com)
  • In an effort to identify a new epigenetic factor that is required for ESC differentiation, the function of SETD5 in ESCs was studied for this thesis. (tmc.edu)
  • In a related but independent study, we show that miR-221-3p and miR-221-5p, which are encoded by the miR-221 gene, are new anti-stemness miRNAs whose expression levels in mouse ESCs are directly repressed by the epigenetic modifier and pluripotent factor PRMT7. (tmc.edu)
  • mechanisms
  • Here, we review recent findings on mechanisms controlling ES cell development. (begellhouse.com)
  • Therefore, it has been proposed that malignant tumors are derived from CSCs with uncontrolled proliferative potential and dysregulation of their mechanisms of differentiation ( 2 ). (pnas.org)
  • assays
  • For more detailed analysis, different cell populations were obtained by selective subset depletion from PBMCs or by sorting using flow cytometry before being assessed in the same assays. (jove.com)
  • Primate
  • 4 . The preparation of CBs of claim 1 comprising cells derived from non-viable primate pre-embryos. (google.es)
  • zygote
  • Because plants do not possess a defined germline, deleterious somatic mutations can be passed to gametes, and a large number of cell divisions separating zygote from gamete formation may lead to many mutations in long-lived plants. (readbyqxmd.com)
  • vivo
  • Germline stem cells (GSCs) in Caenorhabditis elegans provide a tractable in vivo model to address these questions. (readbyqxmd.com)
  • ES cell lines derived from embryos exposed to heat shock or puromycin have retained the capacity for in vivo development, as the stem cells colonised the germline in some chimaeric mice. (bl.uk)
  • Our understanding of how and when breast cancer cells transit from established primary tumors to metastatic sites has increased at an exceptional rate since the advent of in vivo bioluminescent imaging technologies 1-3 . (jove.com)
  • Pathway
  • Pathway analysis generated solely from ECM and growth factor gene expression highlighted morphogenic cell processes within the embryoid bodies, such as cell growth, migration, and intercellular signaling, that are required for primitive tissue and organ developmental events. (jove.com)
  • morphology
  • Slide reading is performed by fluorescent microscopy in dark rooms, and is done by trained technologists who are familiar with the various patterns, in the context of cell cycle and the morphology of interphase and dividing cells. (jove.com)
  • Epithelial
  • Cells of metazoa display two main phenotypes, the ancestral epithelial state and the recent mesenchymal derivative. (biomedsearch.com)
  • Epithelial cells are usually stationary and reside in two-dimensional sheets. (biomedsearch.com)
  • This thesis examined aspects of vibrissa follicle development using immunohistochemistry, and demonstrated that segregation of the follicle dermis and epithelial differentiation were defined by lamin-A antibody. (dur.ac.uk)
  • BrdU labelling of cell division showed regeneration of the epithelial component to be consistent with stem cell location. (dur.ac.uk)
  • Thus, since the follicle dermis produces these cytokines, they may be acting to inhibit the differentiation of follicular epithelial stem cells and/or maintain multipotent stem cell populations in the follicle. (dur.ac.uk)
  • progenitor
  • The umbilical cord is a rich source for progenitor cells with high proliferative potential including mesenchymal stromal cells (also termed mesenchymal stem cells, MSCs) and endothelial colony forming progenitor cells (ECFCs). (jove.com)
  • Briefly, peripheral blood mononuclear cells (PBMCs) are isolated from routine phlebotomy samples and then cultured in defined growth factors to yield a highly proliferative erythrocyte progenitor cell population that is remarkably amenable to reprogramming. (jove.com)
  • efficiently
  • Our protocol and experimental approach enable us to accurately and efficiently quantify the restoration of cytokine production by HIV-specific CD4 T cells from HIV infected subjects. (jove.com)