Pluripotent embryonic stem cells definitive endoderm induction. Medical search. Frequent questions

The epiblast cells are the functional progenitors of soma and germ cells which later differentiate into three layers: definitive endoderm, mesoderm and ectoderm. (wikipedia.org)
Such human fully integrated and complete SEMs recapitulate the organization of nearly all known lineages and compartments of post-implantation human embryos, including the epiblast, the hypoblast, the extra-embryonic mesoderm and the trophoblast layer surrounding the latter compartments. (nature.com)
These include embryonic disc and bilaminar disc formation, epiblast lumenogenesis, polarized amniogenesis, anterior-posterior symmetry breaking, primordial germ-cell specification, polarized yolk sac with visceral and parietal endoderm formation, extra-embryonic mesoderm expansion that defines a chorionic cavity and a connecting stalk, and a trophoblast-surrounding compartment demonstrating syncytium and lacunae formation. (nature.com)
The fertilized oocyte is totipotent, with resultant blastomeres capable of generating all three primary germ layers, ectoderm, mesoderm, and endoderm, in addition to the extra embryonic tissues. (conditionmed.org)
In addition, even in the absence of exogenous supplements, ectoderm, mesoderm, endoderm, and extraembryonic cells differentiated following the induction of collective cell migration at the colony edge by removing the ring-barrier. (biomedcentral.com)
Our findings suggest that collective cell migration can be an effective way to drive the segregation of mesoderm and endoderm lineages, and cell fate decisions of hiPSCs. (biomedcentral.com)
Human pluripotent stem cells (hPSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), are widely used in regenerative medicine and experimental models for studying development and diseases because of their pluripotent potential to differentiate into all cell lineages: endoderm, ectoderm, and mesoderm germ layers [ 1 , 2 , 3 ]. (biomedcentral.com)
Pluripotent stem cells (PSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), are potentially unlimited cell sources for cellular therapies due to the unique capacities of PSCs to self-renew indefinitely and differentiate into cells from all three germ lineages (ectoderm, mesoderm, and endoderm) [ 1 ]. (biomedcentral.com)
During gastrulation anterior endoderm (AE) provides cardiogenic signals that act on adjacent mesoderm , resulting in induction of cardiac precursors. (xenbase.org)

These cells are called epiblast-derived stem cells (EpiSCs) and have several different cellular and molecular characteristics with Embryonic Stem Cells (ESCs). (wikipedia.org)
Conversely, XX cells in ESCs are both active and can produce chimera when inserted into a blastocyst. (wikipedia.org)
The cellular status of human ESCs (hESCs) is similar to primed state mouse stem cells rather than naïve state. (wikipedia.org)
Scientists have been able to demonstrate the induction of EpiSC-like cells in vitro from mouse ESCs, which are referred to as Epiblast-like cells (EpiLC). (wikipedia.org)
Recently, overexpression of PR-domain Zinc Finger Protein 14 (PRDM14) in EpiLC was shown to cause a reversion back to an ESC-like state (with levels of Alkaline Phosphatase staining recovered to that observed in ESCs as well as more ESC-like cell morphology), with Klf2 being required for the mechanism to occur. (wikipedia.org)
Here, we present a quantitative and comprehensive study of the abundance levels of histone PTMs during the differentiation of mouse embryonic stem cells (ESCs) using mass spectrometry (MS). We observed dynamic changes of histone PTMs including increased H3K9 methylation levels in agreement with previously reported results. (biomedcentral.com)
Embryonic stem cells (ESCs) are self-renewing, pluripotent cells derived from the inner cell mass (ICM) of a blastocyst [ 1 , 2 ]. (biomedcentral.com)
Dorsomorphin dihydrochloride also inhibits bone morphogenetic protein (BMP) type I receptors (ALK2, ALK3 and ALK6), promotes cardiomyogenesis in mouse embryonic stem cells (ESCs) in vitro and promotes neural differentiation of hPSCs as part of a chemical cocktail. (tocris.com)

Surfactant is produced by alveolar type II cells which can be differentiated in vitro from patient specific induced pluripotent stem cell (iPSC)-derived lung organoids. (nature.com)
Gene therapy has been utilized successfully to repair or inactivate mutations in animal models of monogenic human diseases 19 as well as human in vitro cells 12 . (nature.com)
Owing to their manifold roles in health and disease, there have been intense efforts to synthetically generate blood vessels in vitro from human pluripotent stem cells (hPSCs). (bvsalud.org)
How can we specifically generate either arterial or venous endothelial cells (ECs) from hPSCs in vitro? (bvsalud.org)
These findings reveal a set of cellular changes that may represent the earliest markers of in vitro hESC transition to an epithelial phenotype, before the induction of gene expression networks that guide hESC differentiation. (northwestern.edu)
Pluripotent cells can be differentiated into many different cell types in vitro. (biomedcentral.com)
Un rôle majeur des prostaglandines dans l'hématopoïèse, ainsi mis en évidence, a pu être étendu à la souris chez qui un traitement par la prostaglandine PGE2 provoque la multiplication des CSH in vivo et in vitro, un résultat qui génerera très probablement des applications cliniques. (hypotheses.org)
We discuss what is known about the distinct metabolic states captured in vitro by the 2-cell-like, naïve, blastocyst-like, formative, and primed states of pluripotency. (conditionmed.org)
2019). Akin to the dynamic nutrient requirements of the developing embryo, discrete in vitro cell states have distinct metabolic profiles (Zhou et al. (conditionmed.org)
It is only through understanding embryonic metabolism and development that we can derive and maintain different in vitro stem cell states for disease modeling and therapies. (conditionmed.org)
On the other hand, even if 3D aggregate culture methods are able to recapitulate in vitro the cell-extracellular matrix interactions properly observed in vivo, and the synthetic/natural matrix and scaffolds have biochemical and mechanical properties, in order to mimic the native extracellular matrix, both of these systems possesses some limitations and some methodological improvements are needed. (razavihospital.ir)
Results: The processes by which re-aggregated adult single cell types or adult and embryonic explanted tissues are able to recapitulate embryogenesis in vitro, when cultured in adhesion or embedded in 3D gels, is not surprising and is clearly under the control of a reminiscent cellular memory which recapitulates early developmental stages. (razavihospital.ir)
Moscona A. The Development in Vitro of Chimeric Aggregates of Dissociated Embryonic Chick and Mouse Cells. (razavihospital.ir)
Kurosawa H. Methods for inducing embryoid body formation: in vitro differentiation system of embryonic stem cells. (razavihospital.ir)
Human induced pluripotent stem cells (hiPSCs) provide an in vitro system to identify the impact of cell behavior on the earliest stages of cell fate specification during human development. (biomedcentral.com)

Embryo-like models with spatially organized morphogenesis and structure of all defining embryonic and extra-embryonic tissues of the post-implantation human conceptus (that is, the embryonic disc, the bilaminar disc, the yolk sac, the chorionic sac and the surrounding trophoblast layer) remain lacking 1 , 2 . (nature.com)
Much of this process relies on the morphogenesis of the extra-embryonic tissues and the effect this has on the organization of embryonic cells. (nature.com)

abstract = "The molecular events leading to human embryonic stem cell (hESC) differentiation are the subject of considerable scrutiny. (northwestern.edu)

The pluripotency of EpiSCs is primed pluripotency: primed to differentiate into specific cell lineages. (wikipedia.org)
Thus, somatic cells (primitive streak) and PGCLCs are related-yet distinct-lineages segregated by temporally-dynamic signaling. (bvsalud.org)

My work over the past ten years has centered on pluripotent stem cells (PSCs, which include embryonic and pluripotent stem cells), which can generate any of the hundreds of diverse cell types in the body. (stanford.edu)

Pluripotency in EpiSCs is essentially different from that of embryonic stem cells. (wikipedia.org)
Pluripotency factors including NANOG are continuously expressed during the transition from pluripotency to posterior epiblast to PGCs, thus bridging pluripotent and germline states. (bvsalud.org)
We explore the recently described metabolic surge event that occurs as pluripotency is lost and stem cells commit to differentiate. (conditionmed.org)
To establish protocols for the safe and efficient differentiation of healthy cells for therapies, we must develop a better understanding of the dynamic continuum of metabolic states that span pluripotency and differentiation, and how to influence them. (conditionmed.org)
Molecular cues for pluripotency, genetic reprogramming of adult stem cells, and widely multipotent adult cells. (razavihospital.ir)

Moscona A. Cell suspensions from organ rudiments of chick embryos. (razavihospital.ir)
The T-box transcription factor eomesodermin (Eomes) has been implicated as an important component in germ layer induction and patterning in vertebrate embryos. (ox.ac.uk)
In the US, where a portion of the population is opposed to destruction of human embryos to obtain stem cells, what avenues are open to scientists for obtaining pluripotent cells that do not offend the moral sensibilities of a significant number of citizens? (asu.edu)
To address some of the limitations of these models, here we used two complementary ex vivo models based on Xenopus embryos: pluripotent animal cap explants expressing Gata4 transcription factor and conjugates of gastrula -stage AE with animal caps (AC). (xenbase.org)

Shih CC, Forman SJ, Chu P, Slovak M. Human embryonic stem cells are prone to generate primitive, undifferentiated tumors in engrafted human fetal tissues in severe combined immunodeficient mice. (razavihospital.ir)
In this study, we investigated the role of autophagy and apoptosis in Newcastle disease virus (NDV)-infected chicken cells and tissues. (immune-source.com)
In bottom line, we confirmed that autophagy brought about by genotype VII NDV infections was important for virus-like replication, NDV-induced apoptosis, and cell survival in buy 480-11-5 chicken cells and tissues. (immune-source.com)
Therefore, we propose that damage to and subsequent release of mtDNA elicits a protective signalling response that enhances nDNA repair in cells and tissues, suggesting that mtDNA is a genotoxic stress sentinel. (regenerativemedicine.net)
The progressive generation of chick and mouse axial tissues - the spinal cord, skeleton and musculature of the body - has long been proposed to depend on the activity of multipotent stem cells. (silverchair.com)

Although many differentiation protocols in the literature have been successful in mimicking lung development from stem cells, there has not been an examination of how a specific mutation impacts the differentiation process including its effects on the early endoderm, as well as the proximal and distal lung epithelial cell populations in the lung organoids. (nature.com)
Here, we employed a human pancreatic differentiation platform complemented with an shRNA screen in human pluripotent stem cells (PSCs) to identify potential drivers of early endoderm and pancreatic development. (mdpi.com)

Mouse naive embryonic stem cells have recently been shown to give rise to embryonic and extra-embryonic stem cells capable of self-assembling into post-gastrulation structured stem-cell-based embryo models with spatially organized morphogenesis (called SEMs) 3 . (nature.com)
Collective cell migration modulates self-organized fate patterning decisions in iPSC-derived gastrulation-stage meso-endoderm. (biomedcentral.com)

They are 3-dimensional, complex, multicellular structures that can be derived from patient specific human induced pluripotent stem cells (hiPSCs). (nature.com)
In the present study, we demonstrate that functional CD34 + progenitor cells can be efficiently produced from human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) by combined modulation of 2 signaling pathways. (ashpublications.org)

However, it has been notoriously challenging to guide PSCs to differentiate into a pure population of a given cell type. (stanford.edu)
To address this challenge, I mapped the cascade of branching lineage choices through which PSCs differentiate into various endodermal and mesodermal cell types. (stanford.edu)
Cell Stem Cell) from PSCs. (stanford.edu)
Controlled expansion and differentiation of pluripotent stem cells (PSCs) using reproducible, high-throughput methods could accelerate stem cell research for clinical therapies. (biomedcentral.com)

Here, we generate >90% pure human artery or vein endothelial cells from pluripotent stem cells within 3-4 days. (stanford.edu)

The blastocyst comprises the inner cell mass (ICM), which gives rise to the three primary germ layers and consequently the fetus, and the trophectoderm (TE), which gives rise to the extraembryonic and placental tissue. (conditionmed.org)

Promotes transdifferentiation of hepatocytes from mouse pancreatic cells (Shen et al. (stemcell.com)
Laminin 411 markedly induced the expression of the genes Foxa2 and Sox17 , markers for pancreatic precursor cells, efficiently induced IPC differentiation from MSCs, and up-regulated insulin expression at both mRNA and protein levels. (biomedcentral.com)
One well-established example of transdifferentiation is the conversion of hepatocytes to pancreatic β-cells. (silverchair.com)
Here, we discuss how transcription factors, and their unique position as the gatekeepers of cellular identity, are exploited in cell reprogramming protocols by exploring work focusing on one reprogramming paradigm - the transdifferentiation of hepatocytes to pancreatic beta cells (β-cell). (silverchair.com)

The postcranial axis (i.e. tissue caudal to the head) is then generated over an extended period in a rostral-to-caudal sequence by cells that are derived from the primitive streak and the adjacent epiblast cells, which together eventually form the tail bud. (silverchair.com)

Finally, hPSC-derived PGCLCs can be easily purified by virtue of their CXCR4+PDGFRA-GARP- surface-marker profile and single-cell RNA-sequencing reveals that they harbor transcriptional similarities with fetal PGCs. (bvsalud.org)
hPSC-derived CD34 + progenitor cells further developed to endothelial and smooth muscle cells with functionality. (ashpublications.org)

We have used conditional gene deletion of mouse β-catenin in Sox17-positive embryonic and extra-embryonic endoderm as well as vascular endothelial progenitors to address the function of canonical Wnt signalling in cell lineage formation and patterning. (biologists.com)
Sox17 lineage tracing in the definitive endoderm (DE) shows a cell-autonomous requirement for β-catenin in midgut and hindgut formation. (biologists.com)
A set of markers (GSC, CK18, Gata4, Eomes, and Sox17) point to a mesendodermal nature of the epithelial cells with subsequent commitment to definitive endoderm (Sox17, Cdx2, nestin, and Islet1). (northwestern.edu)

After the blastocyst stage, once an embryo implanted in endometrium (in case of rodent), the inner cell mass (ICM) of a fertilized embryo segregates into two layers: hypoblast and epiblast. (wikipedia.org)
Stem cells derived from epiblast are pluripotent. (wikipedia.org)
Moreover, beyond the potency-state comparison, MLL1 inhibition was also shown to reactivate the silenced X-chromosome which is typically deactivated in post-implantation epiblast stem cells, suggesting an epigenetic reversion back to a more ground-level, naive state. (wikipedia.org)
Many studies have used EpiLCs as suitable analogues for actual post-implantation derived epiblast stem cells, especially in attempts at reversion back to the "naive" state. (wikipedia.org)

Dental pulp stem cells differentiation reveals new insights in Oct4A dynamics. (razavihospital.ir)

The compound can also be used in protocols for the chemical reprogramming of somatic cells to iPSCs.Shown to induce autophagy in cancer cell lines via a mechanism independent of AMPK inhibition. (tocris.com)
Our complete portfolio of stem cell research products and services provides optimized tools for each step of the stem cell workflow: from reprogramming somatic cells to iPSC expansion, validation, and differentiation. (thermofisher.com)
Reprogramming of somatic cells for iPSC generation can be accomplished with a number of technologies. (thermofisher.com)

After differentiating the mutant and corrected cells into lung organoids, we show expression of SFTPB mRNA during endodermal and organoid differentiation but the protein product only after organoid differentiation. (nature.com)
Here, we developed an hiPSC-based model to study the effect of collective cell migration in meso-endodermal lineage segregation and cell fate decisions through the control of space confinement using a detachable ring culture system. (biomedcentral.com)
Furthermore, the induction of collective cell migration at the colony edge using an endodermal induction media enhanced endodermal differentiation efficiency in association with cadherin switching, which is involved in the epithelial-mesenchymal transition. (biomedcentral.com)
Cadherin switching during collective cell migration enhances endodermal differentiation. (biomedcentral.com)

In the mouse, a transporting epithelium is established around the 8-16-cell stage through a process known as compaction where cell definition is lost and the outer cells of the embryo form tight junctions, giving rise to the blastocyst. (conditionmed.org)
Pivotal roles for eomesodermin during axis formation, epithelium-to-mesenchyme transition and endoderm specification in the mouse. (ox.ac.uk)

Our results indicate that the alterations of tight junctions in pluripotent culture precede the beginning of differentiation. (northwestern.edu)

Our results suggest that combined modulation of signaling pathways may be an efficient means of differentiating hPSCs into functional CD34 + progenitor cells. (ashpublications.org)
Therefore, the therapeutic use of appropriate populations derived from stem and progenitor cells has been considered for cell-based therapies for neurodegenerative diseases, primarily Parkinson's disease, and brain injuries. (ehu.eus)
We further provide evidence to support the hypothesis that transcription factors (TFs) required to drive induction, differentiation, and maintenance of midbrain dopaminergic neural progenitor cells which give rise to terminally differentiated SNpc neurons are uniquely involved in both developmental patterning and metabolism, a dual function unlike other TFs that program neurons in other brain regions. (frontiersin.org)
The use of these TFs during induction and differentiation may program ventral midbrain progenitor cells metabolically to higher ATP levels, allowing for the development of those specialized cell processes seen in terminally differentiated cells. (frontiersin.org)

Human pluripotent stem cells, with their ability to proliferate indefinitely and to differentiate into virtually all cell types of the human body, provide a novel resource to study human development and to implement relevant disease models. (mdpi.com)
Generating primordial germ cell-like cells (PGCLCs) from human pluripotent stem cells (hPSCs) advances studies of human reproduction and development of infertility treatments, but often entails complex 3D aggregates. (bvsalud.org)
Here, we summarize how arterial or venous ECs arise during embryonic development. (bvsalud.org)
Stem Cells and Development , 21 (8), 1250-1263. (northwestern.edu)
In particular, the advent of human embryonic stem cells followed by reprograming technologies for generation of induced pluripotent stem cells have instigated studies into modeling human brain development and disease by providing a means to simulate a human tissue otherwise completely or largely inaccessible to researchers. (ehu.eus)
In particular, due to the limited regenerative capacity of the adult nervous system, the therapeutic potential of stem cells has opened up new perspectives for development of treatment strategies for brain repair after injury or disease. (ehu.eus)
Here, we consider the metabolism of the early embryo through development, and look at the nutrient milieu within the developing stem cell niche. (conditionmed.org)
2020). This places metabolism at the forefront of development and cell state decisions. (conditionmed.org)
The developing embryo, from which pluripotent stem cells originate, undergoes a series of dynamic metabolic transitions synchronized to its molecular development. (conditionmed.org)
The stimulus for the change of cell fate originates from extrinsic signals which set a cascade of intracellular processes in motion that eventually terminate at the genome leading to changes in gene expression and the development of alternate gene regulatory networks. (amegroups.org)
The development of the hematopoietic system has long served as model to understand how changes in gene regulatory networks drive the differentiation of the various blood cell types. (amegroups.org)
Embryonic development is tightly controlled at the level of gene expression. (amegroups.org)
Objectives Mesenchymal stem cells (MSCs) play an important role in the development and growth of tumor cells. (immune-source.com)
The development of an efficient IPC induction system is a crucial step for the clinical application of IPCs for DM. (biomedcentral.com)
The use of hPSCs provides great opportunities to model and study embryonic and human development. (biomedcentral.com)
However, creating a culture platform with spatiotemporal control of cell behavior to study signaling dynamics during development remains challenging. (biomedcentral.com)
The identification of key master regulator transcription factors (which distinguish one body part from another) during embryonic development has been central in developing transdifferentiation protocols. (silverchair.com)
Haeckel aggressively argued that the development of an embryo repeats or recapitulates the progressive stages of lower life forms and that by studying embryonic development one could thus study the evolutionary history of life on earth. (asu.edu)

We use our simplified differentiation platform and single-cell RNA-sequencing to achieve further insights into PGCLC specification. (bvsalud.org)
Marker analysis as well as fate-mapping and chimera studies demonstrate for the first time that Eomes is required for specification of the definitive endoderm lineage. (ox.ac.uk)
Collectively, our experiments establish that Eomes is a key regulator of anteroposterior axis formation, EMT and definitive endoderm specification in the mouse. (ox.ac.uk)
Cerberus and hhex are required in anterior endoderm for liver and cardiac specification. (xenbase.org)

Tetraploid rescue experiments reveal that lack of β-catenin in the anterior visceral endoderm results in defects in head organizer formation. (biologists.com)
Surprisingly, wild-type posterior visceral endoderm (PVE) in midgut- and hindgut-deficient tetraploid chimera rescues the posterior body axis truncation, indicating that the PVE is important for tail organizer formation. (biologists.com)

However, all developmental processes originate from pluripotent precursor cell types. (amegroups.org)

The production of terminally differentiated cells from such cells, therefore, requires successive changes of cell fates, meaning that genes relevant for the next stage of differentiation must be switched on and genes not relevant anymore must be switched off. (amegroups.org)

As a stem cell biologist, I aim to understand the mechanisms through which stem cells differentiate into progressively specialized cell types and to harness this knowledge to artificially generate pure populations of desired cell types from stem cells. (stanford.edu)
Since the cell status is primed to differentiate in EpiSCs, however, one copy of the X chromosome in XX cells (female cells) in EpiSCs is silenced (XaXi). (wikipedia.org)
Human embryonic stem cells (hESCs) derived from an early embryo can self-renew indefinitely and differentiate into a variety of cell types. (ashpublications.org)

I then moved my laboratory to Stanford University as a Siebel Investigator and Instructor at the Stanford Institute for Stem Cell Biology & Regenerative Medicine. (stanford.edu)
These established tools enable new discoveries in fields such as immunology, inflammation and stem cell biology. (bdbiosciences.com)
While techniques for cell surface staining are relatively standard, optimal staining for intracellular markers often depends on the biology of the target protein. (bdbiosciences.com)
Stem cell technologies have opened up new avenues in the study of human biology and disease. (ehu.eus)
They therefore represent a more realistic cellular environment for modeling the cell biology and pathology of the nervous system. (ehu.eus)
When this connection was made, it launched a whole new field," says Isidore Rigoutsos, Ph.D., professor of pathology, anatomy, and cell biology and director of the Computational Medicine Center at Thomas Jefferson University. (pharmaceuticalintelligence.com)

This complex process is regulated by the interplay of transcription factors with a chromatin environment, both of which provide the epigenetic information maintaining cell-type specific gene expression patterns. (amegroups.org)
In the developing embryo, TFs regulating the assembly/disassembly of transcriptional complexes and ultimately gene expression, are directed by complex extrinsic signalling processes which connect all cells within a multi-cellular organism to their environment. (amegroups.org)
Choose from Invitrogen gene editing and transfection technologies to build cell models that can better answer biological questions. (thermofisher.com)

Here we extend those findings to humans using only genetically unmodified human naive embryonic stem cells (cultured in human enhanced naive stem cell medium conditions) 4 . (nature.com)

The posterior download drawing made easy dragons fantasy unleash your of self-containing die in reading beobachten stem and andere with Organ-derived Periodontal differentiation. (it-dresden.net)

Promotes osteogenic, adipogenic, and chondrogenic differentiation of mouse mesenchymal cells (Tropel et al. (stemcell.com)
Promotes differentiation of mature hepatocytes from mouse and human embryonic stem (ES) cells (Cai et al. (stemcell.com)
Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. (razavihospital.ir)
The discovery in 2006 that human and mouse fibroblasts could be reprogrammed to generate induced pluripotent stem cells (iPSCs) with qualities remarkably similar to embryonic stem cells has created a valuable new source of pluripotent cells for drug discovery, cell therapy, and basic research. (thermofisher.com)
Recombinant Human Wnt-3a (Catalog # 5036-WN) induces alkaline phosphatase production by the MC3T3-E1 mouse preosteoblast cell line. (novusbio.com)
Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. (novusbio.com)
In addition, mtDNA stress in TFAM-deficient mouse melanoma cells produces tumours that are more resistant to doxorubicin in vivo. (regenerativemedicine.net)

The process of autophagy in mammalian cells involves six principal actions: initiation, nucleation, elongation, closure, maturation, buy 480-11-5 and degradation [27]. (immune-source.com)

Teratoma formation by human embryonic stem cells is site dependent and enhanced by the presence of Matrigel. (razavihospital.ir)
Komura H, Ogita H, Ikeda W, Mizoguchi A, Miyoshi J, Takai Y. Establishment of cell polarity by afadin during the formation of embryoid bodies. (razavihospital.ir)

The investigation of molecular mechanisms underlying transcriptional regulation, particularly in embryonic stem cells, has received increasing attention and involves the systematic identification of target genes and the analysis of promoter co-occupancy. (biomedcentral.com)
The RUNX2/BMI-1/SMAD2/3 complex is associated predominantly with genes not expressed in undifferentiated BG01V cells, consistent with the reported role of those TFs as transcriptional repressors. (biomedcentral.com)
Cellular identity is determined by the transcriptional profile which comprises the subset of mRNAs, and therefore proteins, being expressed by a cell at a given point in time. (silverchair.com)

B) qPCR showing fold difference in expression between Gata4 treated animal caps and control animal caps grown to st. 33, induction of expression of myl7 , nr1h5 , hhex and marker of endoderm foxa2 is shown. (xenbase.org)

Engineering the embryoid body microenvironment to direct embryonic stem cell differentiation. (razavihospital.ir)
Increasingly, complimentary cell culture techniques, which recapitulate the developmental microenvironment, are employed to coax cells to adopt new identities by indirectly regulating transcription factor activity via intracellular signalling pathways. (silverchair.com)

Current differentiation strategies typically generate heterogeneous cell populations unsuitable for basic research or clinical applications. (stanford.edu)
Stem cell research endeavors to generate specific subtypes of classically defined "cell types. (stanford.edu)
We thus efficiently generate artery and vein cells, introduce stem-cell-based toolkits for biosafety-level-4 virology, and explore the arterial tropism and cellular effects of Nipah and Hendra viruses. (stanford.edu)
A better understanding of the levers governing transcription factor activity benefits our ability to generate therapeutic cell types at will. (silverchair.com)
Here, we explore the evolution of reprogramming and directed differentiation approaches within the context of hepatocyte to β-cell transdifferentiation focussing on how the introduction of new techniques has improved our ability to generate β-cells. (silverchair.com)

Since their discovery, stem cells have ushered in a new era in regenerative medicine. (ehu.eus)
1 Institute for Stem Cell Research and MRC Centre for Regenerative Medicine,University of Edinburgh, King's Buildings, West Mains Road, Edinburgh EH9 3JQ,UK. (silverchair.com)

Cells from this region contribute to the neural tube and the somites, as well as to the notochord. (silverchair.com)

Ying QL, Stavridis M, Griffiths D, Li M, Smith A. Conversion of embryonic stem cells into neuroectodermal precursors in adherent Ferro F et al. (razavihospital.ir)
Embryonic precursors of liver and heart , whilst not sharing cellular origin, develop in close proximity through a dynamic series of inductive signaling events. (xenbase.org)

Cell cycle analysis showed an accumulation of tumor cells predominantly in 511296-88-1 manufacture G0/G1 phase with an increase in concentration of TD-MSCs, which was confirmed by increased mRNA expression of cell cycle negative regulator p21. (immune-source.com)
Apoptosis of tumor cells increased significantly as concentration of cocultured TD-MSCs increased. (immune-source.com)
Insulin-producing cells (IPCs) differentiated from mesenchymal stem cells (MSCs) hold great promise in the treatment of DM. (biomedcentral.com)
UC-MSCs were then cultured in the IPC induction and differentiation medium in the presence of laminin 411. (biomedcentral.com)
Mesenchymal stem cells (MSCs) have gained interest because of its potential application in regeneration medicine and cytotherapy. (biomedcentral.com)

Promotes osteogenic, adipogenic, and chondrogenic differentiation of human mesenchymal cells (Jaiswal et al. (stemcell.com)

Fluorescent antibodies specific for cell surface markers can be combined with markers of apoptosis, proliferation and protein phosphorylation to determine which cell subsets respond to various stimuli or treatments. (bdbiosciences.com)
In addition, many fluorescent antibodies specific for key cell surface markers have been tested in several buffer systems to save time, sample and money. (bdbiosciences.com)
These simplified basic ChIP and novel Sequential ChIP protocols were successfully tested with a variety of antibodies with human embryonic stem cells, generated a number of novel observations for future studies and might be useful for high-throughput ChIP-based assays. (biomedcentral.com)

To establish the importance of specific cell signaling pathways to the production of pro-inflammatory cytokines from LPS-stimulated human monocytes, the NF-B inhibitor, Bay11, the p38 inhibitor, SB203580, the PI3K inhibitor, LY294002, and 607737-87-1 supplier the JNK inhibitor, SP 600125, were added to adherent human monocytes cultured in RPMI 1640 made up of 10% AB+ serum in flat bottom 96 well plates. (morainetownshipdems.org)
Such solutions are readily obtainable for two-dimensional adherent cell cultures, due to defined geometries with low flow rates, which enable precise characterization of fluid flow in microfluidic systems. (biomedcentral.com)

Analyze stem cells and their derivatives using a wide range of cellular and molecular characterization methods, as validation is critical in iPSC research. (thermofisher.com)

Generating human artery and vein cells from pluripotent stem cells highlights the arterial tropism of Nipah and Hendra viruses. (stanford.edu)

Here we show the differentiation of patient specific iPSCs derived from a patient with SFTPB deficiency into lung organoids with mesenchymal and epithelial cell populations from both the proximal and distal portions of the human lung. (nature.com)
While western blotting and other methods are useful for the examination of single proteins expressed by entire cell populations, flow cytometry allows the detection of multiple proteins simultaneously at the level of individual cells. (bdbiosciences.com)

Alveolar type II (ATII) epithelial cells synthesize, secrete and recycle all components of surfactant and dysfunction in surfactant metabolism can result in a variety of pediatric lung diseases including respiratory distress syndrome and interstitial lung disease 3 . (nature.com)

Differentiation of human pluripotent stem cells (hPSCs) into functional cell types is a crucial step in cell therapy. (ashpublications.org)
We offer choices in integration-free reprogramming technologies and services that fit your research and translational needs including our Cell Therapy Systems (CTS) Products, which are designed for clinical and translational research applications. (thermofisher.com)
From feeder-based and feeder-free media to optimized passaging reagents and substrates to media for stem cell therapy research, Gibco products enable you to culture with confidence. (thermofisher.com)
This cell type conversion potentially represents a novel therapy in T1D treatment. (silverchair.com)
We consider how, over the last 20 years, advances in genetic engineering and cell culture techniques have improved the efficiency and efficacy of the transdifferentiation process and brought us closer to a clinically relevant therapy for type 1 diabetes (T1D). (silverchair.com)

J Cell Biol. (razavihospital.ir)

We also review the location and molecular characteristics of these putative stem cells, along with their evolutionary conservation in vertebrates and the signalling mechanisms that regulate and arrest axis extension. (silverchair.com)

In cultured primary fibroblasts and cancer cells, the chemotherapeutic drug doxorubicin causes mtDNA damage and release, which leads to cGAS STING dependent ISG activation. (regenerativemedicine.net)

We specified artery cells by inhibiting vein-specifying signals and vice versa. (stanford.edu)
We also highlight recent studies identifying the cis -regulatory elements such as enhancers at the global level and explain how their developmental activity is regulated by the cooperation of cell-type specific and ubiquitous transcription factors with extrinsic signals. (amegroups.org)

Anatomy55

Organisms12

Diseases4

Chemicals and Drugs72

Analytical, Diagnostic and Therapeutic Techniques and Equipment43

Phenomena and Processes56

Disciplines and Occupations4

Technology, Industry, Agriculture1

Information Science3

Mesoderm9

ESCs8

Vitro15

Extra-embryonic tissues2

Abstract1

Lineages2

Include embryonic1

Pluripotency5

Embryos4

Tissues5

Early endoderm2

Gastrulation2

HiPSCs2

PSCs4

Endothelial Cells1

Germ layers1

Pancreatic4

Primitive1

HPSC-derived2

Sox173

Epiblast4

Differentiation reveals1

Somatic cells3

Endodermal4

Epithelium2

Tight junctions1

Progenitor4

Development18

Specification4

Visceral2

Precursor1

Terminally differentiated cells1

Differentiate3

Biology6

Gene3

Naive1

Posterior1

Mouse7

Mammalian1

Formation2

Transcriptional3

Foxa21

Microenvironment2

Generate5

Regenerative2

Neural1

Precursors2

MSCs5

Mesenchymal cells1

Antibodies3

Adherent2

Cellular and molecular1

Arterial1

Populations2

Epithelial1

Therapy5

Biol1

Molecular characteristics1

Fibroblasts1

Signals2