• Induced pluripotent stem cells, iPSCs, are mature, differentiated cells, such as skin cells, that are reprogrammed in the laboratory to be similar to undifferentiated embryonic stem cells. (jove.com)
  • To create iPSCs, mature cells, like skin fibroblasts, are taken from a patient and put into culture. (jove.com)
  • These cells are also pluripotent like embryonic stem cells-able to produce all cell types-and are therefore called induced pluripotent stem cells (iPSCs). (jove.com)
  • iPSCs are potentially valuable in medicine, because a patient who needs a particular cell type-for instance, someone with a damaged retina due to macular degeneration-could receive a transplant of the required cells, generated from another cell type in their own body. (jove.com)
  • To create iPSCs, mature cells such as skin fibroblasts or blood cells from a person are grown in culture. (jove.com)
  • It is still being studied whether iPSCs are truly equivalent to embryonic stem cells, but they appear to be similar and can produce cells from all three germ layers of the body. (jove.com)
  • As with other types of stem cells, scientists are learning how to promote the differentiation of specific cell types from iPSCs efficiently, so that the needed cell types can be produced in adequate quantities. (jove.com)
  • In previous studies, induced pluripotent stem cells (iPSCs) differentiated into imDCs, and sinomenine (SN) was used to inhibit the maturation of imDCs. (newswise.com)
  • The effects of iPSCs-imDCs and SN-iPSCs-imDCs on T-cell stimulatory function, and regulatory T (Treg) cell proliferative function in vitro were analyzed by mixed lymphocyte reaction. (newswise.com)
  • This study focused on exploring sinomenine (SN) to promote differentiation of induced pluripotent stem cells (iPSCs) into imDCs (SN-iPSCs-imDCs), and found SN-iPSCs-imDCs with worse donor-specific T-cell stimulatory function, and higher regulatory T-cell proliferative function in vitro and in vivo to induce high immune tolerance. (newswise.com)
  • Moreover, compared to their respective cells, iPSCs and iPSC EVs share a greater proportion of proteins, while the PD-MSC proteome appears to be more specific. (unlp.edu.ar)
  • To address these questions, particularly in a human-relevant system, we have focused upon the use of human induced pluripotent stem cells (iPSCs) to assess their ability to give rise to RGCs, with a particular emphasis upon ipRGC differentiation. (indianactsi.org)
  • We found that iPSCs can be effectively differentiated into retinal cells, including the formation of 3D retinal organoids. (indianactsi.org)
  • Human pluripotent stem cells, including ESCs and iPSCs, are highly sensitive cells and undergo apoptosis during these routine procedures. (nih.gov)
  • In vitro differentiation of GS-2 iPSCs into hematopoietic stem and progenitor cells was done using Op9 feeder layers and specified media. (biomedcentral.com)
  • GS-2 iPSCs showed the capacity to differentiate into cells of the hematopoietic lineage. (biomedcentral.com)
  • Differentiated human induced pluripotent stem cells (iPSCs) following differentiation protocol. (cellimagelibrary.org)
  • Cells from the AML-infiltrated skin from an M6 patient were infected with a lentivirus carrying OCT4, SOX2, KLF4 and C-MYC to induce iPSCs. (bvsalud.org)
  • the differentiation potential of iPSCs was determined by embryoid body -formation and teratoma -formation assays. (bvsalud.org)
  • ALP staining confirmed that these cells exhibited positive staining and had the characteristics of iPSCs. (bvsalud.org)
  • These progenitors which are derived from either embryonic stem cells (ESCs) or healthy induced pluripotent stem cells (iPSCs) express wild-type levels of a-syn, thus making them equally susceptible to developing Lewy bodies over time. (lu.se)
  • The advent of iPSCs has opened up the possibility to graft patient-specific cells which most likely would circumvent the need for immunosuppression. (lu.se)
  • Gastric and small intestinal organoids differentiated from human pluripotent stem cells (hPSCs) have revolutionized the study of gastrointestinal development and disease. (bioregate.com)
  • We aim to develop a cell culture platform to automatically differentiate human induced pluripotent stem cells (hiPSCs) for lab-on-a chip applications. (dim-elicit.fr)
  • The implementation of human pluripotent stem cells (hiPSCs) in cell therapy has an extraordinary potential but faces many practical challenges, including costs associated with growth media and factors. (authorea.com)
  • There is an immediate need to establish an optimized culture platform to direct the differentiation of hiPSCs into germ layers in a defined nutritional microenvironment to generate cost-effective and robust therapeutics. (authorea.com)
  • The aim of this study was to identify the optimal nutritional environment by mimicking the in vivo concentrations of three key factors (glucose, pyruvate and oxygen) during the spontaneous differentiation of hiPSCs derived from cord blood, which greatly differ from the in vitro expansion and differentiation scenarios. (authorea.com)
  • A design of experiments (DoE) was used to investigate the interaction between these three variables during the spontaneous differentiation of hiPSCs. (authorea.com)
  • Our findings present a novel approach for efficient directed differentiation of hiPSCs through the manipulation of media components whilst simultaneously avoiding the usage of growth factors thus reducing costs. (authorea.com)
  • Human induced pluripotent stem cells (hiPSCs) have demonstrated great potential for hyaline cartilage regeneration. (mdc-berlin.de)
  • However, current approaches for chondrogenic differentiation of hiPSCs are complicated and inefficient primarily due to intermediate embryoid body formation, which is required to generate endodermal, ectodermal, and mesodermal cell lineages. (mdc-berlin.de)
  • We report a new, straightforward and highly efficient approach for chondrogenic differentiation of hiPSCs, which avoids embryoid body formation. (mdc-berlin.de)
  • We differentiated hiPSCs directly into mesenchymal stem /stromal cells (MSC) and chondrocytes. (mdc-berlin.de)
  • In the present study, we demonstrate that functional CD34 + progenitor cells can be efficiently produced from human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) by combined modulation of 2 signaling pathways. (ashpublications.org)
  • Human induced pluripotent stem cells (hiPSCs) and human embryonic stem cells (hESCs) are promising candidate cell sources for regenerative medicine. (jci.org)
  • However, despite the common ability of hiPSCs and hESCs to differentiate into all 3 germ layers, their functional equivalence at the single cell level remains to be demonstrated. (jci.org)
  • Here, we used single cell analysis to resolve the gene expression profiles of 362 hiPSCs and hESCs for an array of 42 genes that characterize the pluripotent and differentiated states. (jci.org)
  • Comparison between single hESCs and single hiPSCs revealed markedly more heterogeneity in gene expression levels in the hiPSCs, suggesting that hiPSCs occupy an alternate, less stable pluripotent state. (jci.org)
  • hiPSCs also displayed slower growth kinetics and impaired directed differentiation as compared with hESCs. (jci.org)
  • Our results suggest that caution should be exercised before assuming that hiPSCs occupy a pluripotent state equivalent to that of hESCs, particularly when producing differentiated cells for regenerative medicine aims. (jci.org)
  • Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)
  • abstract = "Adoptive cell transfer (ACT) of antigen-specific CD8+ cytotoxic T lymphocytes (CTLs) is a promising treatment for a variety of malignancies 1. (psu.edu)
  • Abstract Background Cardiac differentiation of human-induced pluripotent stem (hiPS) cells consistently produces a mixed population of cardiomyocytes and non-cardiac cell types, even when using well-characterized protocols. (figshare.com)
  • In recent studies we have examined the role of transforming growth factor family members in both pluripotency and differentiation. (qscience.com)
  • EpiSCs share many features with hESCs, and subsequent work supports our hypothesis that hESCs are the human counterparts of EpiSCs, with similar responses to growth factors and mechanisms of pluripotency and differentiation. (qscience.com)
  • Using our integrative proteomics approach and the web-based tool, we uncover a molecular basis for the uncoupling of robust transcription from parsimonious translation in stem cells and propose a method for maintaining pluripotency in vitro. (nature.com)
  • To get a new insight into pluripotency, we employed a plurifaceted experimental design combining expression proteomics with the proteome-wide integral solubility alteration (PISA) assay 7 to compare pluripotent cells with their isogenic progenies and parental cells as well as with allogeneic cells. (nature.com)
  • In stem cells exposed to signals from fetal cells, mechanistic gene networks of upstream regulators decreased pluripotency, while simultaneously inducing mesenchymal and epithelial properties. (elsevierpure.com)
  • The resulting cells maintained a normal karyotype and had high levels of pluripotency markers. (nih.gov)
  • Accordingly, human pluripotent stem cells (hPSCs) with the properties of self-renewal and pluripotency present an ideal cell model for the isolation of pericytes to study their development or the therapeutic effect in pericyte-related diseases. (researchsquare.com)
  • Induced pluripotent stem cells (iPSC) express genes associated with pluripotency, have the capacity for infinite expansion, and can differentiate into cells from all three germ layers. (biomedcentral.com)
  • Writing in the journal Cell Stem Cell , the team reports that more than half of mouse epiblast stem cells treated with the drug reversed course within three days, and regained an embryonic "be anything" state, also called pluripotency. (sciencedaily.com)
  • Past attempts by other teams to restore pluripotency to mouse cells from the epiblast stem cell state have yielded far lower amounts, or non-viable cells. (sciencedaily.com)
  • Then, typically, genes for transcription factors, are delivered by viral vectors into the cell nuclei, where they are incorporated into the genome. (jove.com)
  • The transcription factors then turn on genes that are expressed by embryonic stem cells. (jove.com)
  • Then, genes for multiple transcription factors are delivered into the cells using a viral vector, and the transcription factor proteins are expressed using the cell's machinery. (jove.com)
  • The transcription factors then turn on many other genes that are expressed by embryonic stem cells, returning the cells to an undifferentiated, proliferative, and pluripotent state. (jove.com)
  • This work reveals the importance of BRACHYURY and CDX2 genes as key mediators of embryonic and extraembryonic lineage differentiation in hESCs and EpiSCs. (qscience.com)
  • We thus wondered how cells grow spatially during differentiation, so we labeled cells by expression of marker genes and found that cells expressing the same marker tended to occur in patches. (figshare.com)
  • These transcription factors bind promoters and distant regulatory regions, including E-box elements, of genes whose expression is restricted to muscle cells. (unipv.it)
  • Furthermore, DHA dramatically upregulated the endogenous expression levels of neuroprotective genes ( Bcl-2, Bcl-xl, brain-derived neurotrophic factor, and glial cell-derived neurotrophic factor) and protected against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced apoptosis in iPS-derived neuronal precursor cells. (globalauthorid.com)
  • Furthermore, we found that DHA treatment in iPS cell-grafted rats significantly downregulated the mRNA expression of embryonic stem cell-specific genes (Oct-4 and c-Myc) in the graft and effectively blocked teratoma formation. (globalauthorid.com)
  • We've demonstrated that we don't have to manipulate the pluripotent genes to get to the ground state, but rather that we can block all other options of where the cell 'wants' to go. (sciencedaily.com)
  • Numerous studies have shown that viral vectors can be used to implant osteoinductive growth factor genes directly into the paraspinal muscles or into cells that can subsequently be implanted next to the spine. (medscape.com)
  • The RUNX1 protein turns on (activates) genes that help control the development of blood cells (hematopoiesis). (medlineplus.gov)
  • While t(8;21) is important for leukemia development, a mutation in one or more additional genes is typically needed for the myeloid blasts to develop into cancerous leukemia cells. (medlineplus.gov)
  • Immunocytochemistry and quantitative real-time polymerase chain reaction analysis demonstrated that neuronal differentiation of hiPSC-derived neuronal precursors was enhanced after treatment with 1-2 μg/ml RA-loaded PCANs. (edu.au)
  • In this study, we used iPSC-derived neural progenitor cells (NPCs) from individuals with ASD of known and unknown genetic cause as well as from neurotypical controls to examine the effects of exogenous IL-17a on NPC proliferation, migration and neuronal differentiation, and whether IL-17a and genetic risk factors for ASD interact exacerbating alterations in NPC function. (figshare.com)
  • In conclusion, our results suggest that exogenous IL-17a positively regulates the neuronal differentiation of human NPCs, which may disturb normal neuronal and synaptic development and contribute to MIA-related changes in brain function and behavior. (figshare.com)
  • Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
  • These studies led to our discovery of a novel type of pluripotent epiblast stem cell (EpiSC) from the late epiblast layer of mouse and rat embryos. (qscience.com)
  • One type of pluripotent cell, called a hematopoietic stem cell, can differentiate into a large variety of cells with different functions. (wikipedia.org)
  • We report thermo-responsive retinoic acid (RA)-loaded poly(N-isopropylacrylamide)-co-acrylamide (PNIPAM-co-Am) nanoparticles for directing human induced pluripotent stem cell (hiPSC) fate. (edu.au)
  • My lab integrates traditional and single-nuclei RNA sequencing to elucidate transcriptome-wide changes to RNA metabolism in clinically relevant post-mortem brain tissue and human induced pluripotent stem cell (hiPSC) models of neurological disease including ALS and autism. (ed.ac.uk)
  • Myelodysplastic syndromes Myelodysplastic Syndrome (MDS) are a group of clonal hematopoietic stem cell disorders unified by the presence of distinct mutations of hematopoietic stem cells. (msdmanuals.com)
  • 50 × 10 9 /L) not caused by malignant transformation of a hematopoietic stem cell. (msdmanuals.com)
  • Our focus on mesoderm leads us on to molecular pathways for early human cardiomyocyte differentiation, with a goal of understanding the transcriptional networks responsible for cardiomyocyte identity and using this to generate more homogeneous cardiomyocyte populations for therapeutic applications and drug discovery. (qscience.com)
  • The effect of reactive oxygen species on cardiomyocyte differentiation of pluripotent stem cells. (musc.edu)
  • All-trans retinoic acid (RA) is the most active metabolite of vitamin A. Several studies have described a pivotal role for RA signalling in different biological processes such as cell growth and differentiation, embryonic development and organogenesis. (mdpi.com)
  • In a single experiment, cells that shared a progenitor were more transcriptionally similar to each other than to other cells in the differentiated population. (figshare.com)
  • During liver development, cytokines released from various cell types are critical for stem/progenitor cell differentiation and lineage expansions. (elsevierpure.com)
  • The differentiation mechanisms involving metabolomics products could have an impact on advancing recruitment of stem/progenitor cells during tissue homeostasis. (elsevierpure.com)
  • To directly differentiate to pancreatic cells, use our pancreatic progenitor kit to generate functional PDX1 + /NKX6.1 + cells that can be matured into insulin-producing beta cells. (stemcell.com)
  • hPSC-derived CD34 + progenitor cells further developed to endothelial and smooth muscle cells with functionality. (ashpublications.org)
  • Our results suggest that combined modulation of signaling pathways may be an efficient means of differentiating hPSCs into functional CD34 + progenitor cells. (ashpublications.org)
  • The first part of the thesis (Paper I, II, III) shows the development and improvement of a hESC-based system of for virus-mediated direct reprogramming of human glial progenitor cells into both induced dopaminergic neurons (iDANs) and GABAergic interneurons. (lu.se)
  • However, these cells have limitations, such as rapid maturation and a short lifespan in vivo . (newswise.com)
  • We detect alterations of protein properties in numerous cellular pathways and components including ribosome biogenesis and demonstrate that modulation of ribosome maturation through SBDS protein can be helpful for manipulating cell stemness in vitro. (nature.com)
  • For identifying lineage conversion and maturation, we utilized conventional assays of cell morphology, gene expression analysis and lineage markers. (elsevierpure.com)
  • Regarding vibrational spectroscopy analysis by FTIR, bands related with biomolecules were shown on mPSCs and DKCs spectra, observing distinct differences between cell lineages and maturation stages. (biomedcentral.com)
  • A complex process that involves the proliferation of MEGAKARYOCYTES in BONE MARROW, their differentiation in size and ploidy, and maturation before the release of functional BLOOD PLATELETS. (bvsalud.org)
  • This change in gene activity blocks the maturation (differentiation) of blood cells and leads to the production of abnormal, immature white blood cells called myeloid blasts. (medlineplus.gov)
  • Next, we compared both datasets and found that while iPSC EVs enclose proteins that modulate RNA and microRNA stability and protein sorting, PD-MSC EVs are rich in proteins that organize extracellular matrix, regulate locomotion, and influence cell-substrate adhesion. (unlp.edu.ar)
  • Here we performed a systematic analysis of two different induced pluripotent stem cell lines (iPSC 3.4 and 4.1) and an embryonic stem cell (ESC) line-derived cardiac myocytes at two different developmental stages. (nih.gov)
  • All GS-2 iPSC clones displayed a normal karyotype (46XX or 46XY) and were shown to express the same RAB27A gene mutation that was present in the original somatic donor cells. (biomedcentral.com)
  • Especially in cases where sufficient cells cannot be obtained due to the rarity of the disorder, iPSC models have shown great potential in providing research material to develop new treatments or diagnostics, but also to study the pathophysiology of the diseases [ 10 , 12 ]. (biomedcentral.com)
  • The present study aimed to establish an induced pluripotent stem cell (iPSC) line from acute myelogenous leukemia (AML) cells in vitro and identify their biological characteristics. (bvsalud.org)
  • Our study provided evidence that an iPSC line derived from AML cells was successfully established. (bvsalud.org)
  • However, the approach to obtain embryonic stem cells (ESCs) from patients is not feasible. (psu.edu)
  • In addition, like ESCs, iPS cells possess indefinite proliferative capacity in vitro, and have been shown to differentiate into hematopoietic cells. (psu.edu)
  • Thus, iPS cells have greater potential to be used in ACT-based immunotherapy compared to ESCs or HSCs. (psu.edu)
  • According to their potentiality, embryonic stem cells (ESCs) are classified as totipotent and pluripotent, both of them can differentiate into the three germ lines. (biomedcentral.com)
  • ESCs are obtained from inner cell mass, characterized by unlimited self-renewal and pluripotentiality, which allow them to differentiate into various specialized cells types in a morphologically and functionally way, characteristics that make them attractive for developing differentiated kidney cells (DKCs) [ 9 ]. (biomedcentral.com)
  • Human liver organoids are formed from the assembly of hepatocyte-like cells differentiated from iPS cells, human umbilical vascular endothelial cells and mesenchymal stem cells ( 9 ). (spandidos-publications.com)
  • Here we report the differentiation of human colonic organoids (HCOs) from hPSCs. (bioregate.com)
  • Organoids are three-dimensional (3D) cell cultures containing a self-renewing stem cell population which differentiates into multiple, organ-specific cell types demonstrating key features of the represented organ. (stemcell.com)
  • In this webinar, Dr. James Wells discusses the generation of human intestinal organoids containing a functional enteric nervous system, the differentiation of gastric organoids, and the application of these systems to examine intestinal motility disorders and gastric infection with Helicobacter pylori. (stemcell.com)
  • Induced pluripotent stem (iPS) cells have shown potential for differentiation and may become a resource of functional neurons for the treatment of PD. (globalauthorid.com)
  • Metabolic properties were analyzed to determine the extent of hepatic differentiation. (elsevierpure.com)
  • Cell transplantation studies were performed in mice with drug-induced acute liver failure to elicit benefits in hepatic support and tissue regeneration. (elsevierpure.com)
  • These studies showed signals emanating from fetal liver cells induced hepatic differentiation in stem cells. (elsevierpure.com)
  • The extent of metabolic and synthetic functions in stem cell-derived hepatocytes was sufficient for providing hepatic support along with promotion of tissue repair to rescue mice in acute liver failure. (elsevierpure.com)
  • We concluded that hepatic differentiation of pluripotent stem cells with metabolomics products will be significant for developing therapies. (elsevierpure.com)
  • Bandi, S , Tchaikovskaya, T & Gupta, S 2019, ' Hepatic differentiation of human pluripotent stem cells by developmental stage-related metabolomics products ', Differentiation , vol. 105, pp. 54-70. (elsevierpure.com)
  • See More Use our definitive endoderm (DE) differentiation kit to obtain functional DE cells that are capable of downstream differentiation to hepatic, intestinal, pancreatic and pulmonary cells. (stemcell.com)
  • Undifferentiated mesenchymal stem cells are pluripotent cells that can differentiate into various phenotypes and are a source of osteogenic Cells 1,2 . (bvsalud.org)
  • Directed differentiation of human pluripotent stem cells along the pancreatic endocrine lineage. (ca.gov)
  • Below, we outline a method that is based on published protocols as well as our own experience by which one can differentiate hPSCs along the pancreatic lineage to generate insulin-producing beta-cell-like cells. (ca.gov)
  • Under these conditions, iPS cells begin to differentiate into a hepatocyte lineage. (spandidos-publications.com)
  • To determine mechanisms in these stage-specific lineage interactions, we modeled potential effects of soluble signals derived from immortalized human fetal liver parenchymal cells on stem cells, including embryonic and induced pluripotent stem cells. (elsevierpure.com)
  • Similar uncertainties do Free Two-Day Shipping, Free responsible or academic download Human Embryonic and Induced Pluripotent Stem Cells: Lineage Specific to need robotics, Prime Video, Prime Music, and more. (idealnaja.pl)
  • Australia allowed the other download Human Embryonic and Induced Pluripotent Stem Cells: Lineage Specific Differentiation Protocols to impose software for methods in 1902 and Canada was also system centuries as emotional Goodreads by video until 1922. (idealnaja.pl)
  • Differentiate human pluripotent stem cells (hPSCs) to cells of the endoderm lineage with our specialized cell culture media. (stemcell.com)
  • or movements in a free energy landscape such that lineage choices are paths between stable cell states. (lu.se)
  • Conclusions: This study showed that an osteoblastic cell lineage may be obtained from human bone marrow derived from adherent cells, and that the presence of the rhBMP-4 seems to have an effect during the first stages of differentiation only. (bvsalud.org)
  • Here, we show that the protein level of ClpP is selectively decreased in αSyn-expressing cell culture and neurons derived from iPS cells of PD patient carrying αSyn A53T mutant, and in dopaminergic (DA) neurons of αSyn A53T mice and PD patient postmortem brains. (springer.com)
  • Overexpression of ClpP reduces αSyn-induced mitochondrial oxidative stress through enhancing the level of Superoxide Dismutase-2 (SOD2), and suppresses the accumulation of αSyn S129 phosphorylation and promotes neuronal morphology in neurons derived from PD patient iPS cells carrying αSyn A53T mutant. (springer.com)
  • This study examined whether functional neurons could be efficiently generated from iPS cells using a five-step induction procedure combined with docosahexaenoic acid (DHA) treatment. (globalauthorid.com)
  • DHA treatment facilitated iPS differentiation into tyrosine hydroxylase (TH)-positive neurons in vitro and in vivo and functionally increased dopamine release in transplanted grafts in PD-like animals. (globalauthorid.com)
  • Therefore, our data suggest that DHA plays a crucial role in iPS differentiation into functional DA neurons and that this approach could provide a novel therapeutic approach for PD treatment. (globalauthorid.com)
  • However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
  • We provide detailed protocols for the generation of lentiviral vectors, the transduction of hES-cells, and their differentia- tion into neurons. (lu.se)
  • In this article, we first provide a short history of cell therapy in Parkinson's disease and briefly describe the current state-of-art regarding human stem cell-derived dopamine neurons for use in any patient trial. (lu.se)
  • Several academic and industry efforts are well under owned subsidiary Cyto Therapeutics, it had received way to produce dopaminergic neurons from stem approval by the Australian government to conduct a cells under conditions compliant with use in patients. (lu.se)
  • Without this, the patient cells lost in PD could be replaced by grafted community is left trying to interpret complex scien- immature human dopaminergic neurons [3, 5]. (lu.se)
  • The goal of our research is to develop stem cell-based therapies to replace lost neurons as a treatment for brain diseases. (lu.se)
  • With this knowledge at hand, we become better at controlling the differentiation of human pluripotent cells to subtype-specific neurons which can be used for disease modeling, drug screening, and transplantation, leading to new treatments for several types of brain disorders. (lu.se)
  • We aim to develop novel, lasting therapies for neurodegenerative diseases through the replacement of lost neurons in the brain with new neurons generated from stem cells. (lu.se)
  • Because of this local degeneration of a relatively small population of dopaminergic neurons in the midbrain, PD has been considered an especially interesting candidate for cell-replacement therapy. (lu.se)
  • First, we utilized single cell sequencing to dissect the differentiation of stem cells to midbrain dopaminergic neurons. (lu.se)
  • Direct neuronal reprogramming of a somatic cell into therapeutic neurons, without a transient pluripotent state, provides new promise for the large number of individuals afflicted by neurodegenerative diseases or brain injury. (lu.se)
  • This approach could be potentially applied directly in the brain by targeting resident cells as a source of new neurons. (lu.se)
  • The cells were differentiated into definitive endoderm by using IDE1(induced definitive endoderm). (ac.ir)
  • In the present study, cells morphology and cells viability were evaluated by using a scanning electron microscopy and MTT assay respectively.The results of this study demonstrated the positive effect of 3D cultures, by using suitable factors, on definitive endoderm differentiation.definitive endoderm by using IDE1(induced definitive endoderm). (ac.ir)
  • Understanding transitions from one cell type or state to another is in the focus of modern molecular biology. (nature.com)
  • Pluripotent is also described as something that has no fixed developmental potential, as in being able to differentiate into different cell types in the case of pluripotent stem cells. (wikipedia.org)
  • Understanding how embryonic cells biomolecularly restrict their early developmental potential and promote specific cell commitment helps to provide key insights on cancer plasticity and could improve fertilization protocols. (zeiss.com)
  • Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
  • After analysis of full embryoid bodies (EBs) and cardiac marker (VCAM and cardiac troponin I) positive cells of three lines at 6 conditions (32 different conditions altogether), we found significant SI/R injury-induced cell death in both full EBs and VCAM+ cardiac cells at later stage of their differentiation. (nih.gov)
  • Embryoid bodies (EBs) composed of iPS cells then EBs were seeded on electrospinning nanofiber scaffold (PCL). (ac.ir)
  • Lactate may be applied to produce hepatocytes from iPS cells more efficiently. (spandidos-publications.com)
  • Hepatocytes derived from iPS cells are suitable for transplantation into patients with liver insufficiency and for use in toxicity tests ( 2 ). (spandidos-publications.com)
  • These methods have been previously used to obtain hepatocytes from iPS cells. (spandidos-publications.com)
  • Therefore, methods for obtaining hepatocytes from iPS cells should be further investigated. (spandidos-publications.com)
  • A Hepatocyte selection medium (HSM) was developed to enrich hepatocytes from co-cultures with iPS cells ( 18 ). (spandidos-publications.com)
  • Gene expression profiling and comparison of regulatory networks in immature and mature hepatocytes revealed stem cell-derived hepatocytes represented early fetal-like stage. (elsevierpure.com)
  • In this study, we examined whether human iPS cells, cultured on scaffolds, can differentiate into definitive endodermal cells, as precursor for hepatocytes, pancreatic and lung cells. (ac.ir)
  • Protocols for hepatocyte differentiation from iPS cells have been studied. (spandidos-publications.com)
  • Cells were grown in a directed, stepwise manner following established protocols. (indianactsi.org)
  • We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
  • Finally, we provide protocols for analyzing these cells through flow cytometry and immunocytochemistry. (lu.se)
  • It is known that less-differentiated and central-memory-like (termed highly reactive) CTLs are the optimal population for ACT-based immunotherapy, because these CTLs have a high proliferative potential, are less prone to apoptosis than more differentiated cells and have a higher ability to respond to homeostatic cytokines 2-7 . (psu.edu)
  • Abnormal proliferation, clonal expansion, aberrant differentiation, and diminished apoptosis (programmed cell death) lead to replacement of normal blood elements with malignant cells. (msdmanuals.com)
  • In this review, we focus on the impact of ncRNA post-transcriptional regulatory mechanisms, especially those of microRNAs and lncRNAs, in RA signalling pathways during differentiation and disease. (mdpi.com)
  • Exogenous IL-17a did not induce abnormal proliferation and migration of ASD and control NPCs but, on the other hand, it significantly increased the expression of synaptic (Synaptophysin-1, Synapsin-1) and neuronal polarity (MAP2) proteins in these cells. (figshare.com)
  • During the transition from the naive epiblast to a pluripotent state, over 100 cells are organized into an approximately 60-80 µm diameter pre-implantation mouse embryo. (zeiss.com)
  • Now, they're working to see if the MM-401 eraser technique works with human stem cells that bear some resemblance to mouse epiblast stem cells. (sciencedaily.com)
  • CTLs can recognize malignant cells by interacting tumor antigens with the T cell receptors (TCR), and release cytotoxins as well as cytokines to kill malignant cells. (psu.edu)
  • TH2 cells produce cytokines that will trigger certain B cells. (wikipedia.org)
  • TH1 cells are created to make cytokines, like interferon gamma, that activate macrophages and cytotoxic T lymphocytes (CTLs). (wikipedia.org)
  • Many cytokines are pluripotent, in that each of these compounds can activate specific behavior in some cell types and inhibit other behavior in other cell types. (wikipedia.org)
  • Additionally, we found that substantial cell death occurs during differentiation in a manner that suggested all cells were equally likely to survive or die, suggesting that there is no intrinsic selection bias for cells descended from particular hiPS cell progenitors. (figshare.com)
  • We show that Zeb2 over-expression has a positive impact on skeletal muscle differentiation in pluripotent stem cells and adult myogenic progenitors. (unipv.it)
  • Today, clinical trials using stem cell-derived dopaminergic progenitors have commenced. (lu.se)
  • Next, in order to study the potential of autologous cell replacement therapy we transplanted progenitors derived from a PD patient into a pre-clinical rat model. (lu.se)
  • The successful differentiation of brain pericyte-like cells from human pluripotent stem cells may allow us to study their biological characteristics and their applications in the treatment of pericyte dysfunction-related neurodegenerative diseases, including ischemic stroke, Parkinson's disease (PD) and so on. (researchsquare.com)
  • Are Stem Cell-Based Therapies for Parkinson's Disease Ready for the Clinic in 2016? (lu.se)
  • Recent news of an impending clinical cell transplantation trial in Parkinson's disease using parthenogenetic stem cells as a source of donor tissue have raised hopes in the patient community and sparked discussion in the research community. (lu.se)
  • Based on discussions held by a global collaborative initiative on translation of stem cell therapy in Parkinson's disease, we have identified a set of key questions that we believe should be addressed ahead of every clinical stem cell-based transplantation trial in this disorder. (lu.se)
  • With this background information as a foundation, we then discuss each of the key questions in relation to the upcoming therapeutic trial and critically assess if the time is ripe for clinical translation of parthenogenetic stem cell technology in Parkinson's disease. (lu.se)
  • In particular, we aim to enter a clinical trial with a pluripotent cell product for the treatment of Parkinson's Disease, and are also developing preclinical cell therapies for narcolepsy and dementia. (lu.se)
  • To enter a clinical trial with a stem cell product for the treatment of Parkinson's Disease. (lu.se)
  • However, when the same hiPS cells were differentiated in parallel, we did not observe high transcriptional similarity across differentiations. (figshare.com)
  • In the Kirkeby lab, we further apply advanced human stem cells models and single-cell RNA sequencing to understand how the hundreds of human neural subtypes of cells are formed during embryo development. (lu.se)
  • The protocol we present in the study provides a cranial neural crest originated, fast and robust forebrain pericyte-like cells differentiation method using either human embryonic stem cells or human induced pluripotent stem cells as a starting material. (researchsquare.com)
  • Here, we successfully derive pericyte-like cells with cranial neural crest (CNC) origin from hPSCs (designated as hPSC-CNC PCs), which may represent an ideal cell source for the treatment of pericyte dysfunction-related disorders and help to model the human BBB in vitro for the study of the pathogenesis of such neurological diseases. (researchsquare.com)
  • Mechanistically dissect key RNA metabolism changes causing neural cell dysfunction: Our priorities are to understand the cause and consequence of dysregulated splicing events, and to identify intrinsic master regulators driving transcriptome-wide signatures of neurological disease. (ed.ac.uk)
  • This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)
  • Cells were initially edited by the CRISPR/Cas9 system to express the Thy1 gene at the Brn3b locus and the tdTomato gene at the melanopsin locus. (indianactsi.org)
  • These cells can differentiate into many subtypes once activated by antigen presenting cells (APCs) like dendrites. (wikipedia.org)
  • However, due to difficulties in obtaining a high number of such CTLs from patients, there is an urgent need to find a new approach to generate highly reactive Ag-specific CTLs for successful ACT-based therapies.TCR transduction of the self-renewable stem cells for immune reconstitution has a therapeutic potential for the treatment of diseases 8-10 . (psu.edu)
  • Stem cell-based therapies for Parkinson's dis- ogy company International Stem Cell Corporation ease (PD) are rapidly moving towards clinical trials. (lu.se)
  • To develop novel cell therapies for neurodegenerative diseases. (lu.se)
  • Such stem cell therapies have the potential to repair the damaged brain, making patients independent of daily medications with severe side effects. (lu.se)
  • Through our work on developing novel stem cell therapies for brain diseases, we contribute to the main goal of MultiPark on establishing novel therapeutic approaches for developing more effective symptomatic, new disease-modifying, and/or plasticity-enhancing treatments. (lu.se)
  • The data presented in this thesis may serve as valuable resources to help optimize future cell replacement therapies for patients suffering from PD. (lu.se)
  • We explore the method on three circuits for haematopoiesis and embryonic stem cell development for commitment and reprogramming scenarios and illustrate how the method can be used to determine sequential steps for onsets of external factors, essential for efficient reprogramming. (lu.se)
  • After two days of culture in HSM, the expression levels of α-fetoprotein (AFP) and albumin, which are hepatocyte markers, were upregulated, suggesting that hepatocyte differentiation was initiated ( 19 ). (spandidos-publications.com)
  • By immunostaining for cell type specific markers, I have documented the different stages of retinal differentiation and provided insight into the mechanisms of ipRGC differentiation. (indianactsi.org)
  • In vitro, HCOs express colonic markers and contained colon-specific cell populations. (bioregate.com)
  • To determine which cells are expressing specific cellular markers and how these cells are organized relative to each other requires volumetric imaging of the pre-implantation embryos by confocal microscopy. (zeiss.com)
  • It's the first time that scientists have shown they can get stem cells to revert to their original state by erasing specific labels called epigenetic markers. (sciencedaily.com)
  • In our most recent work we have focused on the role of bone morphogenetic protein (BMP)-4 in the cell fate decision between endoderm and mesoderm, demonstrating the similarity of BMP-induced hESC and EpiSC differentiation to mesoderm induction during mouse gastrulation. (qscience.com)
  • We used an array of self-assembled monolayers to identify peptide surfaces that sustain pluripotent stem cell self-renewal. (nih.gov)
  • Embryonic or adult SCs by definition are characterized by their self-renewal and potentiality, peculiarities that allow them to give rise to more SCs and differentiate into various cell lineages under appropriate conditions. (biomedcentral.com)
  • Malignant transformation usually occurs at the pluripotent stem cell level, although it sometimes involves a committed stem cell with more limited capacity for self-renewal. (msdmanuals.com)
  • However, scientists have found a way to reprogram these mature cells so that they "de-differentiate" and return to an unspecialized, proliferative state. (jove.com)
  • Although the use of hematopoietic stem cells (HSCs) for therapeutic purposes has been widely applied in clinic 11-13 , HSCs have reduced differentiation and proliferative capacities, and HSCs are difficult to expand in in vitro cell culture 14-16 . (psu.edu)
  • Here, we present methods for the generation of T lymphocytes from iPS cells in vitro, and in vivo programming of antigen-specific CTLs from iPS cells for promoting cancer immune surveillance. (psu.edu)
  • Stimulation in vitro with a Notch ligand drives T cell differentiation from iPS cells, and TCR gene transduction results in iPS cells differentiating into antigen-specific T cells in vivo, which prevents tumor growth. (psu.edu)
  • We investigate the role of Zeb2 in skeletal muscle differentiation using genetic tools and transgenic mouse embryonic stem cells, together with single-cell RNA-sequencing and in vivo muscle engraftment capability. (unipv.it)
  • Importantly, the in vivo experiment suggests that DHA induces the differentiation of functional dopaminergic precursors and improves the abnormal behavior of 6-OHDA-treated PD-like rats by 4 months after transplantation. (globalauthorid.com)
  • In vivo differentiation to cells from all three germlines was tested using a teratoma assay. (biomedcentral.com)
  • This invention will significantly improve quality assurance, characterization, and application of pluripotent stem cells in both pre-clinical and clinical settings. (nih.gov)
  • It was suggested that lactate promoted the survival of iPS cells cultured in a medium without glucose and supplemented with galactose. (spandidos-publications.com)
  • Glucose is essential for cell survival and is metabolized to pyruvate ( 13 ). (spandidos-publications.com)
  • This technology includes the use of the combination of the compounds Chroman-1 and Emricasan to achieve virtually 100% cell survival during human pluripotent stem cell passaging, cryopreservation/thawing, and differentiation in 2D and 3D cultures. (nih.gov)
  • The combination of the compounds Chroman-1 and Emricasan permit a higher cell survival rate for pluripotent and differentiating cells compared to commonly used procedures. (nih.gov)
  • Transcription factor RUNX1 promotes survival of acute myeloid leukemia cells. (medlineplus.gov)