Pluripotent cells pluripotent es cells embryonic cell differentiation. Medical search. Frequent questions

Induced pluripotent stem cells, which have been generated from somatic cells by introducing Oct3/4, Sox2, Klf4, and c-Myc, and which are similar to ES cells in morphology, gene expression, epigenetic status and differentiation, can also differentiate into insulin-producing cells. (wjgnet.com)
During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
Its major objective was to produce a gene expression atlas covering the development of ES cells into all three germ layers (ecto-, meso-, and endoderm) and into somatic cells. (europa.eu)
Understanding the genetic pathways underlying differentiation of ES cells to somatic cells would contribute to future therapeutic strategies for degenerative diseases such as heart disease, diabetes and Parkinson's. (europa.eu)
These cell types are capable of unlimited, undifferentiated proliferation in vitro and still maintain the capacity to differentiate into a wide variety of somatic cells. (rndsystems.com)
Generation of reprogrammed somatic cells, known as induced pluripotent stem (iPS) cells, was initially performed by ectopic expression of four transcription factors, however, now this process is becoming more streamlined by improved methods. (edu.au)
Mouse somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) by defined factors known to regulate pluripotency, including Oct4, Sox2, Klf4, and c-Myc. (biomedcentral.com)
Differentiated somatic cells can be reprogrammed into a pluripotent-like state through four defined factors known to regulate pluripotency, including Oct4, Sox2, Klf4, and c-Myc (OSKM) [ 1 , 2 ]. (biomedcentral.com)
Previous studies have demonstrated successful reprogramming of somatic cells into a pluripotent embryonic stem cell-like state via transduction or direct transfection. (usc.edu)
This presentation will describe the current status of strategies to generate induced pluripotent stem (iPS) cells from mouse and human somatic cells for patient-specific disease modeling and cell therapeutic applications. (selectbiosciences.com)

These tissues provide a unique in vitro model to study cardiac physiology with a species-specific advantage over cultured animal cells in experimental studies. (wikipedia.org)
Take an in vitro approach to human neural biomarker discovery and central nervous system (CNS) permeability with human pluripotent stem cell (hPSC)-derived organoids patterned to the choroid plexus, the specialized brain epithelium that forms the blood-cerebrospinal fluid barrier. (stemcell.com)
Hematopoietic Stem Cell and Leukaemic Stem Cell environmental stress responses (In vitro biomimicry of hypoxia, normoxia, hypothermia, hyperthermia, hypoglycemia and hyperglycemia): Effect on cellular growth, cell cycle distribution, apoptosis and cellular metabolism. (imperial.ac.uk)
Protocols for the in vitro differentiation of embryonic stem (ES) cells based on normal developmental processes, have generated insulin-producing cells, though at low efficiency and without full responsiveness to extracellular levels of glucose. (wjgnet.com)
However, it is still unclear to what extent hiPSC may be capable of recapitulating in vitro the cellular and molecular features of the native retina, especially regarding photoreceptor differentiation and functional maturation. (nature.com)
The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)
Researchers at Kumamoto University added VEGF to undifferentiated ES cells and tracked the behavior of the entire genome and epigenome changes over time in vitro. (medicalxpress.com)
Mouse nuclear transfer embryonic stem cells (NT-ESCs) were first established in 2000, and then proved to be able to differentiate either in vivo or in vitro, and give rise to individual tissues through germ line transmission or tetraploid complementation. (benthamscience.com)
Pluripotent embryonic carcinoma (EC) and embryonic stem (ES) cells cultivated as permanent lines offer a new approach for studying cardiogenic differentiation in vitro. (uni-luebeck.de)
We describe cardiogenesis in vitro by differentiating EC and ES cells by way of embryo-like aggregates (embryoid bodies) into spontaneously beating cardiomyocytes. (uni-luebeck.de)
Jeffrey Macklis' laboratory focuses on understanding molecular controls and mechanisms over neuron subtype specification, development, diversity, axon guidance-circuit formation, and pathology in the cerebral cortex (in mice and human neuron/ organoid/ assembloid models), and on applying developmental controls toward both brain and spinal cord regeneration and directed differentiation for in vitro mechanistic and therapeutic modeling. (harvard.edu)
We are working to generate red blood cells in vitro from pluripotent stem cells, however the extremely large cell numbers involved pose unique challenges to culture technology and scale-up. (selectbiosciences.com)
The Biolaminin 521 cell culture substrates successfully recreate the biologically relevant hPSC milieu in vitro . (biolamina.com)
Every cell type has its own unique needs when grown in vitro and stem cells are no exception. (ddw-online.com)
The need to control differentiation of embryonic stem cells in vitro presents another set of challenges. (ddw-online.com)

Embryonic stem (ES) cell pluripotency is thought to be regulated in part by H3K4 methylation. (nih.gov)
Transcriptome analysis revealed the persistent expression of pluripotency genes and underexpression of developmental genes during differentiation in the absence of Kdm5b, suggesting that KDM5B plays a key role in cellular fate changes. (nih.gov)
The developmental progression of embryonic stem cells into specialist cell types involves the activation of lineage-specific programs of gene expression and the silencing of genes involved in maintaining pluripotency. (ca.gov)
A University of Tokyo research group, in collaboration with Uppsala University, revisited the role of bone morphogenetic protein (BMP) signaling in mouse ES cells (a type of stem cell) using next-generation sequencing technology and genome editing tools, and proposed a new model for how BMP maintains naïve pluripotency. (u-tokyo.ac.jp)
Using next-generation sequencing technology and genome editing tools, they showed that the Smad pathway is not essential for maintaining ES cells in a state of naive pluripotency. (u-tokyo.ac.jp)
It thoroughly investigated the unique ability of mouse embryonic stem cells to develop into cells of any organ ("pluripotency"), created new tools for functional genomic studies and thus provided key knowledge to understanding the commitment of cells to differentiate into cells of a particular germ layer. (europa.eu)
FUNGENES identified the gene subsets that are active in self-renewing cells, during the exit from pluripotency and at specific stages of mouse ES cell differentiation. (europa.eu)
To account for this high level of functional variation, verifying the pluripotency of stem cells-from the generation of a new cell line through its expansion for an experiment-is crucial. (genengnews.com)
To address these difficulties, R&D Systems has developed new tools that aid in the verification of stem cell pluripotency during derivation, maintenance, and expansion. (genengnews.com)
Moreover, colony morphology is not truly indicative of pluripotency, since cells that are not fully reprogrammed can have similar morphology. (genengnews.com)
The GloLIVE™ Human Pluripotent Stem Cell Live Cell Imaging Kit and individual GloLIVE antibodies available from R&D Systems allow you to verify the pluripotency status of your stem cells by monitoring the expression of both positive and negative markers ( Figure 1 ). (genengnews.com)
Pluripotency of live human stem cell colonies was verified using a NorthernLights™ (NL) 493-conjugated Mouse Anti-Human SSEA-4 Monoclonal Antibody (green) and a NL557-conjugated Mouse Anti-Human TRA-1-60(R) Monoclonal Antibody (red). (genengnews.com)
Despite its broad use as the most common assay for assessing the pluripotency of stem cells, the teratoma assay has limitations. (genengnews.com)
Using RT-PCR, Western blotting and immunostaining, we show that synemin M is present at both mRNA and protein levels in undifferentiated ES cells as early as pluripotency factor Oct-3/4 and IF keratin 8. (biomedcentral.com)
Although ES cells may be ideal in terms of their pluripotency, from a therapeutic point of view, their great disadvantage is that they are not patient derived. (edu.au)
The various ways in which pluripotent stem cells are generated, particularly in relation to induced pluripotency, are also discussed. (edu.au)
Embryonic stem (ES) cells are able to grow indefinitely (self-renewal) and also have the to differentiate into every mature cell types (pluripotency). (healthyguide.info)
Recent reviews demonstrated that in Ha sido cells many differentiation genes are silenced by Polycomb group (PcG) complexes indicating that the epigenetic rules of gene manifestation is essential for maintaining Sera cell pluripotency (Azuara et al. (healthyguide.info)
The suggestion that epigenetic regulation is an important instrument to control Sera cell pluripotency versus their capacity to differentiate is definitely further supported from the findings the PcG protein Suz12 is required for Sera cell differentiation (Pasini et al. (healthyguide.info)
UTF1 manifestation levels were considerably decreased in all clones tested (Fig. 1 A) whereas manifestation levels of the pluripotency marker Oct4 were not affected (Fig. 1 B). Next DMSO-induced differentiation of wild-type (wt) Renilla and UTF1 KD cells was analyzed (Fig. 1 B). wt and Renilla KD cells differentiated normally which was. (healthyguide.info)
Nanog has been recognized as a critical pluripotency gene in stem cell regulation. (biomedcentral.com)
Background: Recent studies have associated the transcription factors, Oct4, Sox2 and Nanog as parts of a self-regulating network which is responsible for maintaining embryonic stem cell properties: self renewal and pluripotency. (lu.se)
Also, under/over-expression studies of the master regulator Oct4 have revealed that some self-renewal/pluripotency as well as differentiation genes are expressed in a biphasic manner with respect to the concentration of Oct4. (lu.se)
TFs being expressed and the downstream differentiation target been established as candidates to regulate both pluripotency and genes being shut off [11]. (lu.se)
This process accelerates upon acquisition of primed pluripotency and returns to low levels in lineage committed cells. (uni-muenchen.de)
Particularly, by performing transcriptome analysis, we observed that several pluripotent transcriptional factors increase in knockout cell line, which explains the underlying loss of pluripotency in Sirt6-null iPS-like cell line. (biomedcentral.com)
Embryonic stem (ES) cells are derived from the inner cell mass of embryos and they have the capacity to grow indefinitely while maintaining pluripotency. (usc.edu)
Therefore, we hypothesized that Pitx2 is a candidate gene for inhibiting pluripotency gene expression and that Pitx2 knockdown might enhance reprogramming in fibroblast cells. (usc.edu)
This study demonstrated that it is possible to reprogram human dermal fibroblast cells to a pluripotency state although further studies are needed using different reprogramming methods and other pluripotency genes. (usc.edu)
Transcription factors have an important role in the ability of a cell to self-renew and also differentiate into most cell types, also known as pluripotency 1 . (biolegend.com)
While more and more long intergenic non-coding RNAs (lincRNAs) were identified to take important roles in both maintaining pluripotency and regulating differentiation, how these lincRNAs may define and drive cell fate decisions on a global scale are still mostly elusive. (biomedcentral.com)

Human engineered cardiac tissues (hECTs) are derived by experimental manipulation of pluripotent stem cells, such as human embryonic stem cells (hESCs) and, more recently, human induced pluripotent stem cells (hiPSCs) to differentiate into human cardiomyocytes. (wikipedia.org)
Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
They found that the histone code, which alters the transcriptional state of the gene, changes over time as stem cells differentiate into blood vessels in response to a stimulus. (medicalxpress.com)
The defining characteristics of pluripotent stem cells, which include embryonic stem (ES) cells and induced pluripotent stem (iPS) cells, are straightforward: their ability to self-renew and differentiate into ectoderm, mesoderm, and endoderm germ layers. (genengnews.com)
The most widely accepted method for determining whether a population of cells is truly pluripotent is the teratoma assay, that is, verifying in vivo a cell population's ability to differentiate into each of the three germ layers via teratoma formation in mice. (genengnews.com)
Set-up a paradigm for driving hESC to differentiate towards the neural stem cells nnd then neurons. (jcmm.cz)
Naturally occurring Mesenchymal 'adult' stem cells are able to differentiate into a variety of cell types, including bone, cartilage, muscle and fat cells, that can promote healing and reduce pain and inflammation. (kansasrmc.com)
Progenitor cells are similar to embryonic stem cells in their capacity to differentiate into various cell types. (ddw-online.com)
However, progenitor cells can only differentiate into a limited number of cell types. (ddw-online.com)
Stem cells are unique due to their ability to limitlessly self-renew and differentiate into each cell type in the adult body. (biolegend.com)
The capability of these cells to differentiate depends on the stem cell type, the regulation of gene expression by various transcription factors and interaction with the stem cell niche 1,4 . (biolegend.com)
A stem cell is commonly defined as a cell that has the ability to continuously divide and produce progeny cells that differentiate (develop) into various other types of cells or tissues. (bvsalud.org)

These findings were important since they provide new insights as to how such regulatory elements find each other with great speed, selectivity and specificity to induce lineage specific patterns of gene expression both in embryonic stem cells and differentiated progeny. (ca.gov)
Differentiation is a complex process involving actions of ES cell core factors, lineage specific regulators, epigenetic modifications, and chromatin remodelers. (utexas.edu)
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Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)
2007 and that a practical NuRD (nucleosome redesigning and disruption) complex which is definitely involved in nucleosome remodeling is required for the lineage commitment of Sera cells (Kaji et al. (healthyguide.info)
In particular, an excess of Cdx2 over Oct4 determines the trophectoderm lineage whereas an excess of Gata-6 over Nanog determines differentiation into the endoderm lineage. (lu.se)
Citation: Chickarmane V, Peterson C (2008) A Computational Model for Understanding Stem Cell, Trophectoderm and Endoderm Lineage Determination. (lu.se)
Deletion of Jarid2 leads to impaired orchestration of gene expression during cell lineage commitment. (sdbonline.org)
To further understand the epigenetic regulators for specific lineage differentiation from iPS cell would have great significance for potential regeneration therapy and human disease modeling [ 8 ]. (biomedcentral.com)
Identifying the right cocktail of media conditions, supplements and growth factors that successfully drive stem cells toward a desired lineage on a reproducible basis is a time-consuming, iterative exercise. (ddw-online.com)

Background: Bivalent chromatin domains consisting of the activating histone 3 lysine 4 trimethylation (H3K4me3) and repressive histone 3 lysine 27 trimethylation (H3K27me3) histone modifications are enriched at developmental genes that are repressed in embryonic stem cells but active during differentiation. (researchgate.net)
Here we show that bivalent domains and chromosome architecture for bivalent genes are dynamically regulated during the cell cycle in human pluripotent cells. (researchgate.net)
Central to this is the transient increase in H3K4-trimethylation at developmental genes during G1, thereby creating a 'window of opportunity' for cell-fate specification. (researchgate.net)
We found that a large fraction of genes were differentially localized in human thymocytes derived from embryonic stem cells versus human myeloid cells. (ca.gov)
This screen is capable of analyzing the expression of 48 genes simultaneously across dozens of different samples, and our gene list covers all three germ layers that arise during normal embryonic development, the trophectoderm, and epigenetic regulators of chromatin status. (utexas.edu)
Our signature-based screen established several categories of genes based on their comparative functions during the differentiation of ES cells. (utexas.edu)
In some cases, "master" genes have been described which control differentiation versus self-renewal. (justia.com)
Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
At 48 hours, after differentiation into vascular endothelium was determined, a system of transcription was established in which genes unique to vascular endothelial differentiation were induced. (medicalxpress.com)
In particular, FUNGENES identified genes controlling the development of pluripotent embryonic stem (ES) cells into heart cells (cardiomyocytes), nerve cells (neurons), smooth muscle cells, vascular endothelial cells, fat cells (adipocytes), liver cells (hepatocytes) and insulin-producing cells of the pancreas. (europa.eu)
These functional characteristics are in accordance with the expression of α- and β-cardiac myosin heavy chain at early differentiation stages and the additional expression of ventricular-specific MLC-2V and atrial-specific ANF genes at terminal stages demonstrated by reverse transcription polymerase chain reaction (RT-PCR) analysis. (uni-luebeck.de)
A temporally controlled influence of RA on cardiac differentiation and expression of cardiac-specific genes was found. (uni-luebeck.de)
2006 Oddly enough several focus on genes aren't expressed in Ha sido cells. (healthyguide.info)
2006 Interestingly many of the repressed Nanog Oct4 and Sox2 target genes were cooccupied by PcG complexes suggesting that Sera cells are poised to enter differentiation programs but are held in check by PcG-mediated chromatin modifications. (healthyguide.info)
In terms of the gene regulatory network defining cellular potency, Nanog has direct mutual interactions with two other core stem cell specific genes Oct4 and Sox2. (biomedcentral.com)
The model also predicts that reprogramming the network from a differentiated state, in particular the endoderm state, into a stem cell state, is best achieved by over-expressing Nanog, rather than by suppression of differentiation genes such as Gata-6. (lu.se)
RNA sequencing was measured to identify the differential expressed genes due to loss of Sirt6 in somatic and pluripotent cells. (biomedcentral.com)
The underphosphorylated, active form of Rb interacts directly with E2F1 , leading to cell cycle arrest, while the hyperphosphorylated form decouples from E2F1, thus promoting the transcription of genes promoting entry into the S phase. (biolegend.com)
Numerous studies have shown that viral vectors can be used to implant osteoinductive growth factor genes directly into the paraspinal muscles or into cells that can subsequently be implanted next to the spine. (medscape.com)

As ChIP-seq cannot establish physical co-occurrence of two marks on the same allele, admixture of cells that either express (green) or do not express (red) the gene in focus could explain the occurrence of both marks as well as the low expression level in the overall population. (researchgate.net)
In contrast, in the case of ''true'' bivalency, virtually all cells in the population carry both marks simultaneously at the promoter in question, leading to low, if any, expression for that gene in all cells. (researchgate.net)
Histone modifications and chromatin-associated protein complexes are crucially involved in the control of gene expression, supervising cell fate decisions and differentiation. (researchgate.net)
A collaborative research group from Kumamoto University, Kyoto University, and the University of Tokyo in Japan investigated the changes in gene functions that occur when stem cells become vascular cells. (medicalxpress.com)
Moreover, the combination of gene modification and directed differentiation of ESCs provides perfect tool for disease modelling and drug discovery. (benthamscience.com)
The different expression of isoforms H, M and L of synemin in the nervous system raises several questions about the regulation of synemin gene expression during the determination of glial and neuronal cell lineages in the central and the peripheral nervous system (CNS and PNS). (biomedcentral.com)
This selectivity suggests that the commitment of CNS precursor cells to form glia or neuron involves the direct regulation of the single synemin gene. (biomedcentral.com)
Heterogeneous gene expressions of cells are widely observed in self-renewing pluripotent stem cells, suggesting possible coexistence of multiple cellular states with distinct characteristics. (biomedcentral.com)
We present a gene regulatory network model to investigate the bimodal Nanog distribution in stem cells. (biomedcentral.com)
The development of high-throughput RNA sequencing (RNA-seq) at the single-cell level has already led to profound new discoveries in biology, ranging from the identification of novel cell types to the study of global patterns of stochastic gene expression. (wisc.edu)
Although some tools for analysing RNA-seq data from bulk cell populations can be readily applied to single-cell RNA-seq data, many new computational strategies are required to fully exploit this data type and to enable a comprehensive yet detailed study of gene expression at the single-cell level. (wisc.edu)
Expression profiling during ES cell differentiation suggests that the gene, Pitx2, is highly expressed in differentiating cells, which are no longer in an ES cell-like state. (usc.edu)
Using alkaline phosphatase staining to mark ES cells, we identified ES-like colonies that were reprogrammed from fibroblast cells in the plate where OSKM factors were present and the Pitx2 gene was silenced. (usc.edu)
A thorough understanding of the Pitx2 gene will lead to more efficient reprogramming strategies, enabling large-scale production of iPS cells and producing an alternative to embryonic stem cells for use in research and therapy for diabetes, heart failure, Parkinson's disease and other neurodegenerative diseases. (usc.edu)
Top PDF Morphologic and gene expression criteria for identifying human induced pluripotent stem cells. (1library.org)
Persistent donor cell gene expression among human induced pluripotent stem cells contributes to differences with human embryonic stem cells. (1library.org)
RUNX1 regulates CD4 gene transcription during multiple stages of T cell development and represses the CD4 gene in CD4-CD8- (double negative) T cells. (biolegend.com)

Mouse embryonic fibroblasts may be used to maintain and expand pluripotent stem cells in an undifferentiated state. (rndsystems.com)
Mouse embryonic fibroblasts isolated from transgenic Oct4-GFP reporter mice with or without Sirt6 were used for reprogramming by Yamanaka factors. (biomedcentral.com)
Negligible immunogenicity of induced pluripotent stem cells derived from human skin fibroblasts. (1library.org)

This 3D scaffold, along with collagen - a major component of the cardiac extracellular matrix - provides the appropriate conditions to promote cardiomyocyte organization, growth and differentiation. (wikipedia.org)
Support the growth and differentiation of human pluripotent stem cells by using Vitronectin XF™, a defined and xeno-free cell culture matrix. (stemcell.com)
The practice of dentistry is likely to be revolutionized by biological therapies based on growth and differentiation factors that accelerate and/or induce a natural biological regeneration. (bvsalud.org)

ES cells are pluripotent cells, which mean that they can give rise to all differentiated cell types derived from all three germ layers. (bvsalud.org)

Human pluripotent stem cells are differentiated into cardiomyocytes (hPSC-CMs) in culture through a milieu containing small-molecule mediators (e.g. cytokines, growth and transcription factors). (wikipedia.org)
What makes characterization challenging is that heterogeneity within a given stem cell population can cause variability in the differentiation into important downstream lineages, such as cardiomyocytes and hepatocytes. (genengnews.com)
Permanent cultures of cardiac cells described so far have limited value for studying cell biology and pharmacology of the developing heart because of the loss of proliferative capacity and cardiac-specific properties of cardiomyocytes during long-term cultivation. (uni-luebeck.de)
Whereas early differentiated cardiomyocytes are characterized by action potentials and ionic currents typical for early pacemaker cells, terminally differentiated cardiomyocytes show action potentials and ionic currents inherent to ventricular-, atrial- or sinus nodal-like cells. (uni-luebeck.de)
We conclude that ES cell-derived cardiomyocytes provide an excellent cellular model to study early cardiac development and to perform pharmacological and embryotoxicological investigations. (uni-luebeck.de)
Evaluation of the cardiotoxicity of mitragynine and its analogues using human induced pluripotent stem cell-derived cardiomyocytes. (1library.org)
Efficient and scalable purification of cardiomyocytes from human embryonic and induced pluripotent stem cells by VCAM1 surface expression. (1library.org)

This has catalyzed research in both early embryogenesis as a model system for mammalian development as well as regenerative medicine as a renewable source of unspecialized cells which can be converted into nearly any cell type required by a patient. (utexas.edu)
Regenerative medicine has made remarkable progress due to research with embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. (medicalxpress.com)
Pluripotent stem cells have great potential for use in regenerative medicine and are an important tool for understanding developmental and disease mechanisms. (genengnews.com)
Stem cells are emerging as an important source of material for diseases in regenerative medicine. (benthamscience.com)
The study of biology of stem cells is the hallmark of the recent emerging field of regenerative medicine and medical biotechnology. (benthamscience.com)
Such an approach is highly relevant to regenerative medicine since it allows for a rapid search over the host of possibilities for reprogramming to a stem cell state. (lu.se)
In regenerative medicine today, adult stem cells hold the most promise for personal cell-based therapies. (kansasrmc.com)
Macklis is the Max and Anne Wien Professor of Life Sciences, and Professor of Stem Cell and Regenerative Biology, in the Department of Stem Cell and Regenerative Biology, and Center for Brain Science, Harvard University. (harvard.edu)
Stem cell-based regenerative medicine shows much promise. (selectbiosciences.com)
The remarkable potential of stem cells to generate several hundred differentiated cell types is driving their use for regenerative medicine and for supporting the traditional drug discovery and development process (Figure 1). (ddw-online.com)
Literature review: This review summarizes current knowledge, barriers, and challenges in the clinical use of adult stem cells, scaffolds, and growth factors for the development and evaluation of regenerative endodontic therapies. (bvsalud.org)
Regenerative endodontic procedures can be defined as biologically based procedures designed to replace damaged structures, including dentin and root structures, as well as cells of the pulp-dentin complex. (bvsalud.org)
The major areas of research that might have application in the development of regenerative endodontic techniques are (a) postnatal stem cells, (b) scaffold materials, (c) morphogen/growth factors, (d) implantation. (bvsalud.org)
The most valuable cells for regenerative endodontics are postnatal or adult stem cells. (bvsalud.org)
They may also serve as an unlimited source of therapeutically active cells for regenerative medi- cine. (lu.se)

To take human organ generation via BC and transplantation to the next step, we reviewed current emerging organ generation technologies and the associated efficiency of chimera formation in human cells from the standpoint of developmental biology. (frontiersin.org)
Our study shows a developmental stage specific regulation of synemin isoforms in ES cells and its neural derivatives. (biomedcentral.com)
Generation of Pluripotent Stem Cells and their Developmental Potential" by Brock J. Conley, Mark Denham et al. (edu.au)
During cardiomyocyte differentiation three distinct developmental stages were defined by expression of specific action potentials and ionic currents measured by the whole-cell patch-clamp technique. (uni-luebeck.de)
Development: For advances in developmental biology and stem cells. (lu.se)

However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
These findings revealed that RUNX1 acts as a tumor suppressor for myeloid leukemia and is crucial for the development and terminal differentiation of several blood cell lineages 2,3 . (biolegend.com)

Overexpression of embryonic transcription factors in stem cells could efficiently induce their differentiation into insulin-expressing cells. (wjgnet.com)
Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)
The ED50 is less than 30 ng/mL as determined by its ability to induce alkaline phosphatase production by mouse chondrogenic ATDC-5 cells. (anhsci.com)
We hypothesize that ovarian cancer cells will induce human ovarian TVM expression. (nih.gov)

Induced pluripotent stem cells (iPSCs) hold great potential for the modelling of these diseases or as potential therapeutic agents. (nature.com)
These induced pluripotent stem cells (iPSCs) can be generated by forced expression of the four sets of transcription factors, Oct4, Sox2, Klf4, and c-Myc (OSKM). (usc.edu)

ES cells have been an invaluable resource for advancing fundamental understanding of global transcriptional and epigenetic regulations, signaling pathways, and noncoding RNA in mammalian systems. (utexas.edu)
Genetic background drives transcriptional variation in human induced pluripotent stem cells. (1library.org)
We have identified anti-cancer effects of Huaier, and its unique characteristics of 1) no side effects or toxicity, 2) in dose dependent manner, 3) causing specific cancer cell death with simultaneous tissue regeneration, 4) miRNA-mediated transcriptional control on the rescue of multiple signal transduction, by dependent on individual genomic potential. (fortunejournals.com)

However, the molecular mechanisms of how ES cells are differentiated remain much less understood. (utexas.edu)
State-of-the art technologies are used to investigate global molecular changes that arise during neuronal fate specification and differentiation from pluripotent embryonic stem (ES) cells. (yale.edu)
Exploring the genetic, molecular and biological features of neural differentiation process from embryonic and patient-specific stem cells may offer an unprecedented chance to understand normal and abnormal human brain development and diseases, with implications for drug development and potential cellular replacement therapy. (yale.edu)
The molecular mechanisms which explain the paradoxical roles of BMP-4 in pluripotent stem cells remain unknown. (u-tokyo.ac.jp)
Stemfactor BMP-4 is a recombinant protein expressed and purified from human 293 cells as a glycosylated homodimer with a molecular mass of 34 kDa. (anhsci.com)
It will quite effectively cater to the needs of molecular biologists, molecular geneticists, cell and molecular biologists, animal, plant, and crop geneticists, synthetic biologists, biotechnologists, and researchers involved with the fields of stem cell and molecular aspects of cancer research. (caister.com)
Since the discovery of induced pluripotent stem (iPS) cells, the molecular mechanism underlying the reprogramming process has been an active area of research. (biomedcentral.com)
To demonstrate the power of this technology, we will generate a molecular disease fingerprint allowing differentiation between three clinically indistinguishable yet biochemically distinct disease pathways underlying the deadly brain cancer glioblastoma multiforme. (nih.gov)
Jeong, Y & Mangelsdorf, DJ 2009, ' Nuclear receptor regulation of stemness and stem cell differentiation ', Experimental and Molecular Medicine , vol. 41, no. 8, pp. 525-537. (elsevierpure.com)
Molecular motors lie at the heart of biological processes from DNA replication to cell migration. (nih.gov)

During the past year we have identified the folding patterns of human embryonic stem cells to iPS cells derived from human B cells. (ca.gov)
Briefly, iPS cells derived from human B cells were generated as follows. (ca.gov)
Human B cells were isolated from human cord blood and transduced with viral vectors expressing Oct4, Sox2, KLF4 and c-Myc. (ca.gov)
Next the interactome of the iPS cells was determined and compared to that of human embryonic stem cells. (ca.gov)
Using an ensemble of computational strategies we found that the majority of genomic regions in iPS cells derived from human B cells was indistinguishable from that of human embryonic stem cells. (ca.gov)
Specifically, we found that approximately 700 genomic regions located throughout the genome showed differential nuclear positioning upon comparing iPS cells derived from human lymphoid cells to that of human embryonic stem cells. (ca.gov)
These data indicate that the majority of the genome derived from that of human embryonic stem cells shows a similar pattern in chromatin folding as compared to that of iPS cells derived from human B cells but that they differ from each other in a subset of genomic regions. (ca.gov)
Here, we extended the approach to describe the genome topologies of differentiated human lymphoid and myeloid cells derived from human induced pluripotent stem cells. (ca.gov)
Specifically, we have generated differentiated human myeloid and DP thymocytes from induced pluripotent stem cells (iPS). (ca.gov)
We plan to scale up the in situ HiC described here to chart the 3D-genomes of human lymphoid and myeloid cells in order to more to determine genomic regions that are differentially localized in the heterochromatic versus euchromatic compartments. (ca.gov)
We also would aim to describe in detail the 3D-architecture of lymphoid and myeloid genomes that are derived from human induced pluripotent stem cells. (ca.gov)
During the past three years we have identified the folding patterns of human embryonic stem cells to iPS cells derived from human B cells, differentiated progeny and mechanisms that orchestrate the folding patterns of genomes. (ca.gov)
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NZBIndex is forever born in our download Human Embryonic of different Usenet family scientists for different issues are sure. (idealnaja.pl)
Moreover, it has recently been shown that mouse and human ES cells can develop into a three-dimentional (3D) optic cup in culture that remarkably resembles the embryonic vertebrate eye 20 , 21 . (nature.com)
Here we have succeeded in inducing human iPSC to recapitulate the main steps of retinal development and to form fully laminated 3D retinal tissue by exploiting the intrinsic cues of the system to guide differentiation ( Supplementary Fig. 1 ). (nature.com)
However, researchers have indicated that BMP-4 also induces differentiation of other pluripotent stem cells, including human ES cells. (u-tokyo.ac.jp)
His postdoctoral studies at the Institute for Stem Cell Research at the University of Edinburgh focused on neural induction and differentiation of mouse and human embryonic stem (ES) cells. (cureparkinsons.org.uk)
He also has a major focus on driving forward the use of human ES and iPS cells for use in cell replacement therapies for Parkinson's. (cureparkinsons.org.uk)
3. developed new embryonic stem cell-based approaches to screening of small candidate molecules for therapeutic applications in human diseases. (europa.eu)
We also offer defined culture media, which are specifically optimized for use with human or rodent pluripotent stem cells. (rndsystems.com)
The kit contains a set of azide-free, fluorochrome-conjugated GloLIVE antibodies that recognize two positive markers and one negative marker of human pluripotent stem cells. (genengnews.com)
The human body is made up of about 220 different kinds of specialized cells such as nerve cells, muscle cells, fat cells and skin cells. (benthamscience.com)
Embryonic stem cells (ESCs) can grow infinitely and give rise to all types of cells in human body, thus of tremendous therapeutic potentials for a variety of diseases, such as Parkinson's disease, spinal cord injury, and diabetes. (benthamscience.com)
In human embryonic development, BMP-4 is a critical signaling molecule required for the early differentiation of the embryo and establishment of a dorsal-ventral axis 2,3 . (anhsci.com)
In cultured stem cells, BMP-4 plays a distinct role in mouse and human embryonic stem (ES) cells. (anhsci.com)
In contrast, BMP-4 induces extra-embryonic trophoblast differentiation in human pluripotent stem cells 5 . (anhsci.com)
Stemfactor BMP-4 was expressed in and purified from human 293 cells. (anhsci.com)
Use Vitronectin XF™ with mTeSR™1 , mTeSR™ Plus , TeSR™-E8™ , or TeSR™-AOF medium to provide a defined culture system for the maintenance of human embryonic stem (ES) cells and human induced pluripotent stem (iPS) cells under feeder-free conditions. (stemcell.com)
This project is further to elucidate the role and the mechanism of NNAT in neural induction in human ES cells. (jcmm.cz)
This project will shed light on the complex networks that regulate neural specification in human ES (hES) cells and may provide paradigms for the derivation of more homogenous NSC cells from induced pluripotent stem (iPS) cells for stem cell replacement therapy. (jcmm.cz)
We used Sharpr-MPRA to test 4.6 million nucleotides spanning 15,000 putative regulatory regions tiled at 5-nucleotide resolution in two human cell types. (wisc.edu)
and unique human subcellular biology relevant to ALS/MND, SCI, and ASD via directed subtype differentiation in iPS-derived multicomponent assembloids with relevant circuitry. (harvard.edu)
We utilized retrovirus transduction to reprogram human dermal fibroblast cells. (usc.edu)
Novel culture and cryopreservation systems for human pluripotent stem cells and neural stem cells will be presented and their advantages and applicability in the production of high quality advanced therapeutic products or functional screening tools for preclinical research discussed. (selectbiosciences.com)
Clinical use of human embryonic stem (hES) cells is already being explored in a several phase1 safety trials. (selectbiosciences.com)
Broad T-cell receptor repertoire in T-lymphocytes derived from human induced pluripotent stem cells. (1library.org)
DNA methylation dynamics in human induced pluripotent stem cells over time. (1library.org)
Human induced pluripotent stem cells differentiation into oligodendrocyte progenitors and transplantation in a rat model of optic chiasm demyelination. (1library.org)
Scalable passaging of adherent human pluripotent stem cells. (1library.org)
Efficient commitment to functional CD34+ progenitor cells from human bone marrow mesenchymal stem-cell-derived induced pluripotent stem cells. (1library.org)
Biolaminin 521 (laminin-521 protein) is a key cell adhesion protein of the natural stem cell niche, expressed and secreted by human pluripotent stem cells (hPSCs) in the inner cell mass of the embryo. (biolamina.com)
Via integrin binding, Biolaminin 521 induces the PI3K/Akt signaling pathway, promoting high survival and robust long-term self-renewal of human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC). (biolamina.com)
Together, Biosilk and Biolaminin 521 create a biologically relevant 3D culture environment for the expansion and differentiation of human ES and iPS cells. (biolamina.com)
The laminin 521 protein is also one of the most common laminins expressed in the human adult body and therefore Biolaminin 521 also supports the culture of specialized tissue cell types, such as pancreatic cells, liver cells, vascular cells, cardiac and skeletal muscle cells, and different neural cells. (biolamina.com)
Stem cells, and in particular human embryonic stem (ES) cells, have earned a reputation for being labour intensive and difficult to grow and control in culture. (ddw-online.com)
F urther complicating matters, human ES cells are typically co-cultured with feeder layers of mouse fibroblast cells. (ddw-online.com)
Several growth factors have been identified that promote growth of human ES cells in culture, most notably basic fibroblast growth factor (bFGF). (ddw-online.com)
However, the use of bFGF for human ES cell culture, particularly at the high levels used by some for 'feeder-free' culture, must be considered carefully, as bFGF may drive ES cells towards differentiation. (ddw-online.com)
Xeno-free media that do not rely upon high levels of bFGF have been developed that contain only humanised or synthetic components and are engineered to support the undifferentiated growth and expansion of human ES cells. (ddw-online.com)
Use of progenitor cell lines can eliminate the need to culture challenging human ES cells. (ddw-online.com)
For example, neural progenitor cells derived from a human ES cell line are easily propagated and require less handling than human ES cells. (ddw-online.com)
A new tumor model, combining human embryonic stem cells (ESC) and tumor cells, develops abundant human vessels. (nih.gov)
Finally, our preliminary data suggest that the ESC-ovarian tumor model has human tumor vascular cells. (nih.gov)
We hypothesize that the ESC ovarian tumor model, with human vascular cells, will provide an ideal microenvironment to support human stem cell growth. (nih.gov)
If successful, this will create a murine tumor model that nearly completely reproduces the human tumor microenvironment with human tumor stroma, vessels and tumor stem cells. (nih.gov)
Rb homeostasis is also essential for self-renewal and survival of human embryonic stem cells 10 . (biolegend.com)
Based on multiple RNA-Seq datasets, we systematically identified 300 human embryonic stem cell lincRNAs (hES lincRNAs). (biomedcentral.com)
Of which, one forth (78 out of 300) hES lincRNAs were further identified to be biasedly expressed in human ES cells. (biomedcentral.com)
As the first global profiling and annotating of human embryonic stem cell lincRNAs, this work aims to provide a valuable resource for both experimental biologists and bioinformaticians. (biomedcentral.com)
Combining a comprehensive collection of human embryonic stem cell RNA-Seq datasets with Human BodyMap 2, we validated that 295 previously annotated lincRNAs were expressed in multiple human embryonic stem cell samples and further identified five novel hES lincRNAs through de novo assembling. (biomedcentral.com)
Moreover, we surveyed and manually screened published hES RNA-Seq datasets in several public repositories, resulting in a list of 31 wild-type human embryonic stem cell samples. (biomedcentral.com)
Here, we employ recent developments in stem cell biology to show that hsa-let-7c mediates important regulatory epigenetic functions that control the development and functional activity of human induced neuronal cells (iNs). (nih.gov)
We show that overexpression of hsa-let-7c in human iNs derived from induced pluripotent stem (iPS), as well as embryonic stem (ES), cells leads to morphological as well as functional deficits including impaired neuronal morphologic development, synapse formation and synaptic strength, as well as a marked reduction of neuronal excitability. (nih.gov)
The possible donor-host rejection of human ES cells is another concern 3 . (bvsalud.org)
To date, four types of human dental stem cells have been isolated and characterized: (i) dental pulp stem cells (DPSCs) 12 (ii) stem cells from exfoliated deciduous teeth (SHED) 21 (iii) stem cells from apical papilla (SCAP) 31 (iv) periodontal ligament stem cells (PDLSCs) 28 . (bvsalud.org)
Sozzi E, Nilsson F, Kajtez J, Parmar M, Fiorenzano A. Generation of Human Ventral Midbrain Organoids Derived from Pluripotent Stem Cells . (lu.se)
Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)
We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)
However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
Although possible, these strategies are often complicated to transfer to human cells due to technical issues (9 ), and only a few successful cases have been described ( 10, 11 ). (lu.se)
Here we describe how this system can be used for human embry- onic stem cells. (lu.se)

The present invention also provides methods of using the differentiated cells of the present invention for therapeutic purposes. (justia.com)
The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
Kuldip S. Sidhu , " Frontiers in Pluripotent Stem Cells Research and Therapeutic Potentials Bench-to-Bedside ", Bentham Science Publishers (2012). (benthamscience.com)
As we learn more about the workings of stem cells and increase our ability to apply medical breakthroughs, we are realizing the remarkable therapeutic potential stem cells can have to help the body heal itself. (kansasrmc.com)
These studies provide insights into the therapeutic potential of the NR superfamily in stem cell therapy and in treating stem cell-associated diseases (e.g., cancer stem cell). (elsevierpure.com)
The opportunities and challenges of development of stem cell-based therapeutic interventions for joint surface repair for the treatment and prevention of osteoarthritis will be discussed. (selectbiosciences.com)
The use of mouse feeder layers and animal serum are particularly problematic in the culturing of stem cells for possible therapeutic applications. (ddw-online.com)

The let-7 family of miRNAs has been shown to regulate neuronal differentiation, neural subtype specification, and synapse formation in animal models. (nih.gov)

The FUNGENES project aimed to achieve a detailed basic understanding of stem cell self-renewal and differentiation. (europa.eu)
Genetic regulation involving various transcription factors results in the self-renewal and differentiation properties of stem cells. (elsevierpure.com)

Very few studies have addressed the functional roles of the bimodal heterogeneity of Nanog expression in the differentiation and specification process of stem cells. (biomedcentral.com)

These findings provide functional insight into the role for KDM5B in regulating ES cell differentiation and as a barrier to the reprogramming process. (nih.gov)
Stem cells are undifferentiated cells which can give rise to a succession of mature functional cells. (justia.com)
All these diseases are characterised by the irreversible loss of functional cells. (europa.eu)
The idea to use transplants of dopa- ment of protocols that allow generation of fully functional mine-producing cells to substitute for the lost midbrain and safe midbrain dopamine neurons from stem cells. (lu.se)

Embryonic stem (ES) cells are derived from the embryo and are pluripotent, thus possessing the capability of developing into any organ, cell type or tissue type. (justia.com)
Induced pluripotent stem cells, another types of pluripotent stem cells derived from any tissue by reprogramming and are the homologous source of stem cells. (benthamscience.com)
Here, proximity-anchored in situ spectral coding amplification (ProxISCA) is proposed for multiplexed imaging of RNA mutations, enabling visual typing of brain gliomas with different pathological grades at the single-cell and tissue levels. (bvsalud.org)
A. Adult stem cells are "blank," or undifferentiated, cells that your body creates to replace dying cells or heal damaged tissue. (kansasrmc.com)
Stem cells include a diverse number of toti-, pluri-, and multi-potent cells that play important roles in cellular genesis and differentiation, tissue development, and organogenesis. (elsevierpure.com)
Increasing evidence shows that certain NRs function in regulating stemness or differentiation of embryonic stem (ES) cells and tissue-specific adult stem cells. (elsevierpure.com)
The key elements of tissue engineering are stem cells, morphogen, and a scaffold of extracellular matrix. (bvsalud.org)
Postnatal stem cells have been sourced from umbilical cord blood, umbilical cord, bone marrow, peripheral blood, body fat, and almost all body tissues, including the pulp tissue of teeth 8 . (bvsalud.org)
These dental stem cells are considered mesenchymal stem cells (MSCs) and possess different levels of capacities to become specific tissue forming cells. (bvsalud.org)

Thus, it has been argued that the observed bivalency simply reflects cellular heterogeneity arising from the averaging of cells that carry either, but not both, marks at a given locus (Fig. 2). (researchgate.net)
This full potentiality makes them particularly suitable for developing cell replacement therapies or establishing cellular model systems. (edu.au)
These results suggest the co-existence of two cellular "sub-states" within the pluripotent stem cell state: high-Nanog sub-state and low-Nanog sub-state, rather than one well-defined, homogenous pluripotent cellular state. (biomedcentral.com)
This presentation describes the current state of the stem cells marketplace as it relates to their utilization for pharmaceutical screening as well as their development for cellular therapy. (selectbiosciences.com)
For decades scientists have been trying to reproduce cell culture environment for the closest mimicry of the natural cellular "embedding" within a living organism. (selectbiosciences.com)
STAT3 protein belongs to a group of intracellular transcription factors that mediate a variety of functions such as cellular differentiation, proliferation, and apoptosis. (biolegend.com)

In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
During the creation of new blood vessels, the vascular endothelial growth factor (VEGF) protein differentiates stem cells into vascular endothelial cells and stimulates them to create new blood vessels. (medicalxpress.com)
The protein GATA2, which binds to ETV2 and supports vascular endothelial differentiation, was induced immediately thereafter. (medicalxpress.com)

Alternatively, stem cell cultures can be grown on extracellular matrix extracts and supplemented with conditioned medium from mouse fibroblast cultures. (ddw-online.com)

Established a hESC-derived neural differentiation model. (jcmm.cz)

Mathematical modeling of Hematopoietic Stem Cell and Leukaemic Stem Cell culture systems. (imperial.ac.uk)
OP9 feeder cell co-culture system was used to measure the hematopoietic differentiation from mouse ES and iPS cells. (biomedcentral.com)
RUNX1-deficient mice fail to generate hematopoietic stem cells. (biolegend.com)

Epigenetic plasticity underpins cell potency, but the extent to which active turnover of DNA methylation contributes to such plasticity is not known and the underlying pathways are poorly understood. (uni-muenchen.de)

Embryonic stem cells are stem cells derived from the inner cell mass of an early, preimplantation stage embryo known as a blastocyst. (bvsalud.org)

UTF1 KD perturbed Sera and embryonic carcinoma (EC) cell differentiation whereas their ability to self-renew was unaffected. (healthyguide.info)

Our in vivo analysis also confirmed the expression of synemins H/M in multipotent neural stem cells in the subventricular zone of the adult brain, a neurogenic germinal niche of the mice. (biomedcentral.com)
These findings represent the first evidence that synemins could potentially be useful markers for distinguishing multipotent ES cells from undifferentiated neural stem cells and more committed progenitor cells. (biomedcentral.com)

Mouse embryonic stem (ES) cells are pluripotent cells, meaning that they can give rise to all tissues in the body. (utexas.edu)
In the field of stem cell research, BMP-4 has been shown to be a key factor in the blood serum, which, together with leukemia inhibitory factor (LIF), sustains mouse embryonic stem (ES) cells in their undifferentiated state. (u-tokyo.ac.jp)
The research group of Professor Kohei Miyazono and Associate Professor Daizo Koinuma at the Graduate School of Medicine, the University of Tokyo, collaborating with Professor Hiroyuki Aburatani at the Research Center for Advanced Science and Technology, the University of Tokyo, and researchers at Uppsala University, Sweden, has revisited the role of BMP signaling in a classical model of mouse ES cells. (u-tokyo.ac.jp)
He continues, "We also confirmed the significance of the MEK5-ERK5 pathway in mouse ES cells from a different genetic background. (u-tokyo.ac.jp)
The teratoma assay is also inherently variable, depending on the site of cell inoculation and the mouse strain used. (genengnews.com)
BMP-4 supports LIF as a positive factor for mouse ES cell self-renewal 4 . (anhsci.com)
We have examined the expression profile of synemin isoforms in mouse pluripotent ES cells and during their neural differentiation induced by retinoic acid. (biomedcentral.com)
Our analysis of mouse development from embryonic day 5 to 15 (E5 to E15) has demonstrated that synemin M mRNA is produced at E5 as early as nestin and vimentin mRNA, prior to the appearance of the H isoform. (biomedcentral.com)
Launch Mouse embryonic stem (Ha sido) cells produced from the internal cell mass of blastocyst embryos be capable of self-renew and so are pluripotent. (healthyguide.info)
We have recently identified a novel intrinsic factor, Neuronatin (Nnat), which acts as a neural initiator in mouse embryonic stem (ES) cells and also promotes the neural fate in Xenopus embryos (Lin et al, submitted). (jcmm.cz)
Here we use metabolic labelling with stable isotopes and mass spectrometry to quantitatively address the global turnover of genomic methylcytidine (mdC), hydroxymethylcytidine (hmdC) and formylcytidine (fdC) across mouse pluripotent cell states. (uni-muenchen.de)
Sirt1, the mouse homologue of yeast Sir2 deacetylates several non-histone proteins and plays roles in many key functions, including energy metabolism, differentiation, aging, and tumor suppression [ 10 ]. (biomedcentral.com)
But the exact role of Sirt6 in mouse somatic reprogramming and iPS cell differentiation remains unrevealed. (biomedcentral.com)
Use of feeder layers requires two cell types to be maintained in parallel and introduces mouse proteins into the culture system. (ddw-online.com)
To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)

Here we report that hiPSC can, in a highly autonomous manner, recapitulate spatiotemporally each of the main steps of retinal development observed in vivo and form three-dimensional retinal cups that contain all major retinal cell types arranged in their proper layers. (nature.com)
One challenge with characterizing tumor stem cells has been finding appropriate conditions for in vivo growth. (nih.gov)
We therefore propose (3) to isolate ovarian tumor stem cells and grow them in vivo using the ESC ovarian cancer model. (nih.gov)

These antibodies are added to live, unfixed cells in a single-step assay that takes only 30 minutes and has no adverse effects on stemness or proliferation. (genengnews.com)
1998 A recent study suggested a role for PHA-793887 UTF1 in the proliferation rate and teratoma-forming capacity of Sera cells (Nishimoto et al. (healthyguide.info)
Pax6 serves as a regulator in the coordination and pattern formation required for differentiation and proliferation to successfully take place, ensuring that the processes of neurogenesis and oculogenesis are carried out successfully. (biolegend.com)

BMP signalling), ectodermal cells automatically adopt neural fate. (jcmm.cz)

Depending on the source, stem cells can be classified into two broad categories i.e. embryonic stem cells that are derived from embryos and non-embryonic stem cells that are derived from adult and fetal tissues. (benthamscience.com)
The non-embryonic stem cells like adult stem cells are in clinical use for many years and embryonic stem cells are now emerging as an alternative source for the same purpose with huge potentials in drug discovery and toxicological studies. (benthamscience.com)
Adult adipose stem cells from naturally occurring fatty deposits are abundant in quantities. (kansasrmc.com)
Adult stem cells have been used for a variety of medical treatments to repair and regenerate acute and chronically damaged tissues - from joint and muscle injuries to cardiac and pulmonary conditions to autoimmune disorders. (kansasrmc.com)
There are two main types of stem cells, adult and embryonic, which require unique means of collection and deployment. (kansasrmc.com)
Based on their origin, there are two main types of stem cells - embryonic stem cells (ES cells) and postnatal or adult stem cells (AS cells). (bvsalud.org)

Dynamically and epigenetically coordinated gata/ets/sox transcription factor expression is indispensable for endothelial cell differentiation. (medicalxpress.com)
Furthermore, they found that a transcription factor group essential for blood vessel differentiation (ETS/GATA/SOX) has a previously unknown role. (medicalxpress.com)
Schematic representation of the genetic regulatory network linking four transcription factors and EC differentiation. (medicalxpress.com)
Transcription factors SOX and FLI1, both important for endothelial differentiation, were induced between 12 and 24 hours. (medicalxpress.com)
Previously, in the region that controls the function of the transcription factor that promotes differentiation from ES cells to a specific cell type, bivalent modifications of histones such as the accelerator and brake histone marks for transcription were thought to have coexisted. (medicalxpress.com)
Induced pluripotent stem (iPS) cells can be generated by somatic cell reprogramming following the exogenous expression of specific transcription factors (Oct-3/4, KLF4, SOX2, and c-Myc). (rndsystems.com)
2004 Intracellular regulators of Ha sido cell self-renewal consist of Oct4 Sox2 Nanog as well as the lately implicated transcription elements Sall4 Esrrb Tbx3 and Tcl1 (Yuan et al. (healthyguide.info)
Pax6 is a transcription factor present during embryonic development. (biolegend.com)

More recently, advances in medical biology have shown that the genomic state of a somatic cell can be altered or 'reprogrammed' to become pluripotent. (edu.au)
At the core of the network reside Oct4, Sox2 and Nanog, into embryonic stem cells [1,2,3,4,5], have made major inroads which form a self-organized core of the TFs maintaining into stem cell biology. (lu.se)

Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)
Methylation state on the histone tail on the chromatin, histone code, in endothelial cells was read via hi-speed deep sequencer. (medicalxpress.com)

This system allows complete control over the culture environment, resulting in more consistent cell populations and reproducible results in downstream applications. (stemcell.com)

For testing the influence of exogenous compounds on cardiac differentiation the teratogenic compound retinoic acid (RA) was applied during distinct stages of embryoid body development. (uni-luebeck.de)

The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)

It has been suggested that synemin could function as a linker between different cytoskeletal components based on the fact that it interacts with several proteins involved in the organization of the costameres, neuromuscular and myotendinous junctions within striated muscle cells. (biomedcentral.com)
These data demonstrate that UTF1 is a chromatin-associated proteins mixed up in initiation of ES cell differentiation strongly. (healthyguide.info)
Our scientists have developed a wide array of stem cell-focused reagents and resources for many applications including flow cytometry , western blotting , ELISAs , and recombinant proteins for cell differentiation. (biolegend.com)

In the process of blood vessel differentiation, the function of the protein ETS variant 2 (ETV2), which determines the differentiation into vascular endothelium, was first induced within 6 hours of differentiation stimulation. (medicalxpress.com)
Synemin H was produced only in neural precursors when neural differentiation started, concurrently with synemin M, nestin and glial fibrillary acidic protein. (biomedcentral.com)
It has been reported that Sirtuin 6 (Sirt6), a member of the sirtuin family of NAD + -dependent protein deacetylases, is involved in embryonic stem cell differentiation. (biomedcentral.com)
Preliminary reprogramming was assessed by the detection of red fluorescent in cells infected with red fluorescent protein. (usc.edu)
Rb , or retinoblastoma protein, is a key regulator of the cell cycle, particularly during the transition from the G1 to S phases. (biolegend.com)
Within the brain, the protein is involved in the development of specialized cells that process smell. (biolegend.com)

We provide detailed protocols for the generation of lentiviral vectors, the transduction of hES-cells, and their differentia- tion into neurons. (lu.se)
Finally, we provide protocols for analyzing these cells through flow cytometry and immunocytochemistry. (lu.se)

However, it is unclear how H3K4 demethylation contributes to ES cell function and participates in induced pluripotent stem (iPS) cell reprogramming. (nih.gov)
Here, we show that KDM5B, which demethylates H3K4, is important for ES cell differentiation and presents a barrier to the reprogramming process. (nih.gov)
We also observed accelerated reprogramming of differentiated cells in the absence of Kdm5b, demonstrating that KDM5B is a barrier to the reprogramming process. (nih.gov)
To gain new insights into pathogenesis of specific neurological and neurodevelopmental disorders, such as autism, pluripotent stem cells are derived by reprogramming of differentiated, mature cells of affected patients. (yale.edu)
Interestingly, the existence of low-Nanog state dynamically slows down the reprogramming process, and additional Nanog activation is found to be essential to quickly attaining the fully reprogrammed cell state. (biomedcentral.com)
Our modeling results quantitatively show a dual role of Nanog during stem cell differentiation and reprogramming, and the importance of the intermediate state during cell state transitions. (biomedcentral.com)
Our approach offers a general method for analyzing key regulatory factors controlling cell differentiation and reprogramming. (biomedcentral.com)
It provides a framework to explore strategies of reprogramming a cell from a differentiated state to a stem cell state through directed perturbations. (lu.se)
Recent breakthroughs in reprogramming differentiated cells loops. (lu.se)
A computational model of master regulators that are required for successful reprogramming the dynamics of this core network has revealed that it functions as of a differentiated cell into a cell exhibiting stem cell like a bistable switch, which in the on state, corresponds to all these properties. (lu.se)
And later both our group and others reported that Sirt1 can promote the efficiency of reprogramming and maintain characteristics of iPS cell [ 15 , 16 , 17 ]. (biomedcentral.com)
We found that Sirt6 is highly expressed in pluripotent stem cells and also it regulates the efficiency of somatic reprogramming. (biomedcentral.com)
We found that after infection, long and thin fibroblast cells transformed into round ES cell-like colonies and were more transparent 11 days after reprogramming. (usc.edu)
Reprogramming of differentiated cells can be efficiently induced in oocytes. (selectbiosciences.com)

In this capacity, pluripotent stem cells have widespread clinical potential for the treatments of heart disease, diabetes, spinal cord injury, and a variety of neurodegenerative disorders. (rndsystems.com)
The quality of stem cells deployed appear to be related directly to the clinical success and favorable outcomes of a procedure. (kansasrmc.com)
Details are provided about the quantitative market opportunity for various stem cell classes as well as the current state of the clinical trials space wherein stem cell-based therapeutics are being evaluated for therapy in various disease classes. (selectbiosciences.com)

Knocking down synemin in ES cells by shRNA lentiviral particles transduction has no influence on expression of Oct4, Nanog and SOX2, but decreased keratin 8 expression. (biomedcentral.com)
Model simulations demonstrate that the low-Nanog state benefits cell differentiation through serving as an intermediate state to reduce the barrier of transition. (biomedcentral.com)
One hypothesis based on these results is: During the stem cell differentiation process, the low-Nanog state of stem cell functions as the "gate-keeper" state. (biomedcentral.com)

Progenitor cells can be far easier to handle in culture than ES cells. (ddw-online.com)

Retroviral vectors packed with the four OSKM factors were introduced into fibroblast cells. (usc.edu)
Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)

The success achieved over the last decade with islet transplantation has intensified interest in treating diabetes, not only by cell transplantation, but also by stem cells. (wjgnet.com)

These findings will contribute to the development of more appropriate culture conditions for maintaining ES cells and/or induced pluripotent stem (iPS) cells and generation of high-quality iPS cells in more naïve states. (u-tokyo.ac.jp)
Joy then bravely reported this to Dr Kunath, who pursued this idea further and has since worked alongside Joy, Professor Perdita Barran at the University of Manchester and a team of researchers to isolate several substances in the skin which signal Parkinson's, potentially helping clinicians to diagnose the condition earlier and therefore allowing development of drugs to target and treat the condition and protect brain cells before they begin to die. (cureparkinsons.org.uk)
This complex process occurs in several steps and controls the development of pluripotent cells into highly specialised cells of an organism. (europa.eu)

Anatomy53

Organisms19

Diseases7

Chemicals and Drugs77

Analytical, Diagnostic and Therapeutic Techniques and Equipment38

Phenomena and Processes64

Disciplines and Occupations3

Technology, Industry, Agriculture1

Information Science3

Somatic cells10

Vitro15

Pluripotency31

Differentiate13

Lineage11

Genes19

Gene17

Fibroblasts3

Growth and differentiation3

Germ layers1

Cardiomyocytes7

Regenerative15

Developmental5

Terminal differentiation2

Induce5

IPSCs2

Transcriptional3

Molecular10

Human75

Therapeutic7

Neuronal differentiation1

Self-renewal and differentiation2

Specification1

Functional4

Tissue9

Cellular6

Vascular endothelial3

Extracellular1

HESC1

Hematopoietic3

Pathways1

Embryo1

Carcinoma1

Neural stem2

Mouse15

Vivo3

Proliferation3

Fate1

Adult6

Transcription8

Biology2

Endothelial2

Downstream1

Retinoic acid1

Lineages1

Proteins3

Protein6

Protocols2

Reprogramming14

Clinical3

Nanog3

Progenitor cells1

Vectors2

Transplantation1

Development3