Pluripotent cell lines. Medical search. Frequent questions

Here, we report the generation and characterization of induced pluripotent stem cells (iPSCs) derived from fibroblasts and peripheral blood mononuclear cells from three AGS patients with biallelic SAMHD1 mutations. (rcsi.com)
They called the pluripotent stem cells that they produced induced pluripotent stem cells (iPSCs) because they had induced the adult cells, called differentiated cells, to become pluripotent stem cells through genetic manipulation. (asu.edu)
B ) Morphology of induced pluripotent stem cells (iPSC) colonies, ( C ) proliferation rate of iPSCs, and ( D ) photoreceptor primary cilium of organoids were compared between WT and rd16 . (elifesciences.org)
Generate a bank of fibroblasts and corresponding induced pluripotent stem cells (iPSCs) that represent the breadth of healthy adult chronological age from a human cohort extensively phenotyped for key physical and functional measures of biological age. (salk.edu)
Neuronal cells expressing pro-opiomelanocortin (POMC), neuropeptide-Y/agouti-related protein (NPY/AgRP) were generated from human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) obtained from patients with monogenic forms of obesity. (columbia.edu)

Support the growth and differentiation of human pluripotent stem cells by using Vitronectin XF™, a defined and xeno-free cell culture matrix. (stemcell.com)

A team of Harvard Stem Cell Institute (HSCI) scientists has taken an important step toward producing induced pluripotent stem (iPS) cells that are safe to transplant into patients to treat diseases. (harvard.edu)

My thesis research focused on establishing an in vitro model for understanding the molecular neurophysiology of obesity using, as 'proof-of-principle', neurons derived from human pluripotent stem cells (hPSCs) derived from individuals with monogenic forms of obesity. (columbia.edu)

We showed that when an iPS-like cell line, designated 10-22, derived from human recurrent PDAC, was injected into immunodeficient mice, the cells consistently recapitulated preinvasive, pancreatic intraepithelial neoplasia (PanIN) to invasive stages of human PDAC. (elsevierpure.com)
However, the heterogeneous initial populations, different cellular states, and active memory of pancreatic epithelial cells challenge for making iPSC-like lines from human PDAC. (elsevierpure.com)
Ca V 3 channels are ontogenetically downregulated with the maturation of certain electrically excitable cells, including pancreatic β cells. (mdpi.com)
Promotes differentiation of pancreatic cells from human pluripotent stem cells (D'Amour et al. (stemcell.com)
NEW ORLEANS - Two innovative new approaches to replacing pancreatic beta cells offer hope for treating both type 1 and type 2 diabetes in the future, experts say. (medscape.com)
Meanwhile, Dr Cowan's group has already developed a method for generating hundreds of millions of insulin-producing pancreatic beta cells from human pluripotent stem cells. (medscape.com)

Yamanaka worked to find new ways to acquire embryonic stem cells to avoid the social and ethical controversies surrounding the use of human embryos in stem cell research during the late twentieth and early twenty-first centuries. (asu.edu)
Martin GR. Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. (springer.com)
Establishment in culture of pluripotential cells from mouse embryos. (springer.com)
There are two types of stem cell manufacturing: embryonic stem cell production, derived from early-stage embryos, and induced pluripotent stem cell (iPSC) production, which reprograms adult cells to an embryonic-like state. (imarcgroup.com)
The use of embryonic stem cells has been a source of considerable controversy due to its sacrifice of human embryos in the blastocyst stage, which some people view as the destruction of human life . (citizendium.org)
Human embryos fertilized in the ordinary manner and harvested in the blastocyst stage have been used as an extensive source of stem cells for research purposes, and have been shown to possess therapeutic value in laboratory animals. (citizendium.org)
The most infamous study of embryonic stem cells asserted that cloned human embryos had been created via somatic cell nuclear transfer, and stem cells had been generated from these embryos. (citizendium.org)
Ethical objections to the use of human embryonic stem cells revolve around the destruction of human embryos in the blastocyst stage to obtain the stem cells. (citizendium.org)

Human induced pluripotent stem cell (iPSC) lines are a powerful tool for studying development and disease, but the considerable phenotypic variation between lines makes it challenging to replicate key findings and integrate data across research groups. (ca.gov)
To address this issue, we sub-cloned candidate human iPSC lines and deeply characterized their genetic properties using whole genome sequencing, their genomic stability upon CRISPR-Cas9-based gene editing, and their phenotypic properties including differentiation to commonly used cell types. (ca.gov)
We then shared KOLF2.1J with groups around the world who tested its performance in head-to-head comparisons with their own preferred iPSC lines across a diverse range of differentiation protocols and functional assays. (ca.gov)
The European Bank for induced pluripotent Stem Cells performs collection, banking, quality control and distribution of iPSC lines for research purposes. (wikipedia.org)
EBiSC's stated goal is to supply academic, non-profit and commercial researchers with quality-controlled, disease-relevant iPSC lines, data and other services. (wikipedia.org)
It also seeks to promote the international standardisation of iPSC banking practices and to act as a central hub that ensures the sustainability and accessibility of iPSC lines generated by different research organisations. (wikipedia.org)
IPSC lines generated externally can be deposited into EBiSC for storage, banking, quality control and distribution. (wikipedia.org)
In February 2020, the EBiSC catalogue contained iPSC lines representing diseases and conditions such as Alzheimer's disease, Frontotemporal Dementia, Parkinson's disease, Huntington's disease, Dravet syndrome, Bardet-Biedl syndrome, depression and pain, diabetes mellitus, eye diseases and heart disease. (wikipedia.org)
These iPSC lines have been deposited into EBiSC by academic institutions and non-profit and commercial organisations internationally. (wikipedia.org)
The goal of the Initiative is to generate and characterize a large number of human iPSC lines as a resource for the global research community. (genengnews.com)
Deliver 2 clonally expanded mycoplasma free iPSC lines (15 x 300k cells). (lu.se)
Generation of feeder-independent transgene-free iPSC lines from a young-onset Parkinson's disease (YOPD) patient with a homozygous PLA2G6: c.2222G>A (p. (bvsalud.org)

The mutation occurs in hematopoetic stem cells capable of self renewal and additional mutations are associated with clonal progression may occur in progenitor cells conferring a self renewal capability. (standardofcare.com)
The paradox is that the mutation provides advantage at the stem cell level and progenitor cells, with a disadvantage at the level of hematopoietic precursors. (standardofcare.com)
Nkx2.1 progenitor cells at 12 days of differentiation from iPSC integrated into the hypothalamus following injection into the lateral ventricle of NSG mice. (columbia.edu)

The developmental capacity of nuclei taken from differentiating endoderm cells of Xenopus laevis. (springer.com)
The model also predicts that reprogramming the network from a differentiated state, in particular the endoderm state, into a stem cell state, is best achieved by over-expressing Nanog, rather than by suppression of differentiation genes such as Gata-6. (lu.se)
Citation: Chickarmane V, Peterson C (2008) A Computational Model for Understanding Stem Cell, Trophectoderm and Endoderm Lineage Determination. (lu.se)

Our in vivo tracking tools will be broadly relevant and not restricted to studying the in vivo biology of blood forming cells. (ca.gov)
To exploit the full potential of human pluripotent stem cells for regenerative medicine, developmental biology and drug discovery, defined culture conditions are needed. (nih.gov)
The adenoviruses infect the cells" carrying the genes needed for cellular reprogramming, "but are cleared by the cells after a few cell divisions," said Hochedlinger, an assistant professor in Harvard's new inter-school Department of Stem Cell and Regenerative Biology. (harvard.edu)
Human Stem Cell Technology & Biology: A Research Guide and Laboratory Manual integrates readily accessible text, electronic and video components with the aim of effectively communicating the critical information needed to understand and culture human embryonic stem cells. (ecampus.com)
1 Introduction to Pluripotent Stem Cells: Biology and Applications ( Maria Borowski and Gary S. Stein ). (ecampus.com)
Nature Cell Biology. (bdbiosciences.com)
At the core of the network reside Oct4, Sox2 and Nanog, into embryonic stem cells [1,2,3,4,5], have made major inroads which form a self-organized core of the TFs maintaining into stem cell biology. (lu.se)
You can put islet cells into someone with type 1 diabetes and they can leave the hospital 3 days later with no diabetes….But you're not going to get very far with human donors," press briefing moderator Gordon C Weir, MD, professor of medicine at Harvard Medical School, and cohead of the Joslin Diabetes Center's section on islet cell and regenerative biology, told Medscape Medical News . (medscape.com)

Use Vitronectin XF™ with mTeSR™1 , mTeSR™ Plus , TeSR™-E8™ , or TeSR™-AOF medium to provide a defined culture system for the maintenance of human embryonic stem (ES) cells and human induced pluripotent stem (iPS) cells under feeder-free conditions. (stemcell.com)

Currently, we lack markers that we can use to pinpoint the developmental stage of human ES cell derived candidate hematopoietic stem cells (HSC), and whether they have established the correct molecular machinery required for proper function. (ca.gov)
The major shortcoming in generating functional hematopoietic stem cells (HSC) from pluripotent stem cells is the lack of adequate markers for specification and maintenance of functional human HSCs. (ca.gov)
There is clonal proliferation of malignant hematopoietic stem cells, dysregulated cellular differentiation, and compromised tissue function. (standardofcare.com)
A heterogenous group of myeloid disorders characterized by somatically mutated hematopoietic stem cells,the presence of variable peripheral cytopenias, and a broad risk of progression to acute myeloid leukemia (AML). (standardofcare.com)

These lines will serve as tools to identify factor combinations to improve the in vitro differentiation of hESCs to functional blood stem cells. (ca.gov)
The stem cell field witnessed a genuine breakthrough when a combination of solely four transcription factors ( Oct3 / 4 , Sox2 , Klf4 and c-Myc, OSKM ) proved enough to revert, in vitro, the differentiated status of a variety of cell types back to pluripotency, giving rise to so-called induced pluripotent stem (iPS) cells. (springer.com)
In vitro tissue engineering of smooth muscle sheets with peristalsis using a murine induced pluripotent stem cell line. (otago.ac.nz)
I am using human pluripotent stem cells that carry either trisomy 21 or the fmr1 mutation to study the development of the cerebral cortex in vitro. (wisc.edu)
This project was designed to establish an in vitro model for studying hypothalamic cell-molecular physiology in neurons derived from hPSCs. (columbia.edu)
With the recent advances in human-cell cultivation techniques, allowing in vivo -like in vitro long-term functionality, there is a shift in focus towards the mechanistic details of the adverse effects "over time" aimed at a better understanding of the dynamics of biological processes. (brill.com)
In vitro methods, based on human primary cells, cell lines, and genetically modified reporter cell lines, have greatly expanded the scope of in vitro toxicology. (brill.com)
In vitro experiments using NV virus-like particles (VLPs) directly showed NV VLP attachment to HBGA, resulting in VLP internalization into the cell ( 19 ). (cdc.gov)
From these critical studies, putative NV receptors were identified, and thus it was hypothesized that a successful in vitro cell culture system would most likely possess these receptors to support NV replication. (cdc.gov)

These tools will help translate the promise of stem cells to cell based therapies to treat human disease. (ca.gov)
As such, this work could help lead to novel stem cell therapies and shed light on a variety of mental disorders, such as schizophrenia , autism and bipolar disorder, which may stem from problems with development, researchers say. (livescience.com)
Cells from corpses might play a key role in developing future stem cell therapies . (livescience.com)
Rising stem cell research and increasing demand for stem cell therapies are driving the growth of this segment. (globenewswire.com)
Increasing investments in stem cell research, rising chronic disease prevalence, advancements in biotechnology, collaborations between academia and industry, stringent regulations, and expanding awareness of stem cell therapies are stimulating the market growth. (imarcgroup.com)
Apart from this, the increasing awareness and acceptance of stem cell-based therapies among patients and healthcare professionals is augmenting the market growth. (imarcgroup.com)
This driver is fueled by the promising prospects of stem cell therapies in addressing previously untreatable conditions, such as neurodegenerative diseases and spinal cord injuries. (imarcgroup.com)
Stem cell therapies hold immense promise in addressing these ailments by regenerating damaged tissues and organs. (imarcgroup.com)
The idea is that these will be analogous to the O-negative "universal-donor" blood type and could be employed for all cell-based transplantation therapies in patients with immune rejection, said Dr Cowan. (medscape.com)
Are Stem Cell-Based Therapies for Parkinson's Disease Ready for the Clinic in 2016? (lu.se)
Stem cell-based therapies for Parkinson's dis- ogy company International Stem Cell Corporation ease (PD) are rapidly moving towards clinical trials. (lu.se)

13 Characterization of Human Embryonic Stem Cells by Immunofluorescence Microscopy ( Shirwin M. Pockwinse and Prachi N. Ghule ). (ecampus.com)
Detailed information about all of the lines and downloadable files for the corresponding characterization data can be viewed on the HipSci website. (genengnews.com)
basic characterization, including cell authentication (STR), undifferentiated state (FACS) and molecular karyotyping. (lu.se)

The induced pluripotent stem cell (iPSC) line was generated using the integration-free Sendai virus method from peripheral blood mononuclear cells (PBMCs) of a hearing-impaired patient with homozygous GJB2 c.235delC mutation. (nycu.edu.tw)

Importantly, these cells displayed hypothalamic neuronal characteristics, including production and secretion of neuropeptides and responsiveness to metabolic hormones such as insulin and leptin. (columbia.edu)
Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)

Reduces mammosphere-forming efficiency of breast cancer cell lines and ductal carcinoma in situ cells (Farnie et al. (stemcell.com)
A bone biopsy specimen was taken in the Department of Oral and Maxillofacial Surgery and the histopathology revealed pseudo-cystic and ductal mucin pooled spaces lined by mucous cells and squamous or epidermoid cells. (bvsalud.org)

A ) Morphology of Nrl -GFP wild-type (WT) and rd16 retinal organoids differentiated from mouse-induced pluripotent stem cells (iPSC) at various differentiation time points. (elifesciences.org)
cell authentication (STR), pluripotency and undifferentiated state confirmation, molecular karyotype, g-banding and iPS growth pattern and morphology analysis. (lu.se)

Recent news of an impending clinical cell transplantation trial in Parkinson's disease using parthenogenetic stem cells as a source of donor tissue have raised hopes in the patient community and sparked discussion in the research community. (lu.se)
Based on discussions held by a global collaborative initiative on translation of stem cell therapy in Parkinson's disease, we have identified a set of key questions that we believe should be addressed ahead of every clinical stem cell-based transplantation trial in this disorder. (lu.se)
In this article, we first provide a short history of cell therapy in Parkinson's disease and briefly describe the current state-of-art regarding human stem cell-derived dopamine neurons for use in any patient trial. (lu.se)
With this background information as a foundation, we then discuss each of the key questions in relation to the upcoming therapeutic trial and critically assess if the time is ripe for clinical translation of parthenogenetic stem cell technology in Parkinson's disease. (lu.se)
As we have a strong focus on developing cells for cell replacement therapy in Parkinson's disease, we are currently adapting our protocols for GMP compliance through the EU-funded network NeuroStemcellRepair. (lu.se)
In line with this aim, we also perform extensive preclinical validation of the human dopamine neurons derived from our protocol in rat models of Parkinson's disease. (lu.se)

We used an array of self-assembled monolayers to identify peptide surfaces that sustain pluripotent stem cell self-renewal. (nih.gov)
The term stem cell is also used in reference to any adult cells that are capable of assisting in the restoration of adult tissue via self-renewal. (citizendium.org)
Background: Recent studies have associated the transcription factors, Oct4, Sox2 and Nanog as parts of a self-regulating network which is responsible for maintaining embryonic stem cell properties: self renewal and pluripotency. (lu.se)

Establishment of germ-line-competent embryonic stem (ES) cells using differentiation inhibiting activity. (springer.com)
Establishment of an induced pluripotent stem cell line (ZJSHi001-A) from a patient with epileptic encephalopathy carrying KCNB1 Glu330Asp mutation. (nih.gov)

Such tissue renewal may be accomplished via the use of adult stem cells, or embryonic stem cells, which may be derived from a human embryo in the blastocyst stage. (citizendium.org)
However, supporters of embryonic stem cell research frequently contend that even the comparison to abortion is inappropriate, since while a several month old fetus might have sufficient neurological development to be conscious in some meaningful sense, a human embryo in the blastocyst stage has so little development that one can safely conclude that it cannot exist as a conscious being. (citizendium.org)

An aberrant miRNA expression could contribute to cancer development and progression [ 6 , 7 ] and could affect their target genes that are involved in many biological processes, such as cell differentiation, proliferation, apoptosis, metabolism, and development [ 8 ]. (hindawi.com)
Molecular Abnormalities Many factors are involved in causing and permitting the unregulated proliferation of cells that occurs in cancer. (msdmanuals.com)

The Dolly experiment showed that scientists could reprogram the nucleus of somatic cells by transferring the contents of the nucleus into oocytes that have had their nuclei removed, a technique called somatic cell nuclear transfer (SCNT). (asu.edu)

This time, there were twenty-two cell colonies that showed a resistance to G418, meaning that there were colonies in which the cells exhibited embryonic stem cell properties. (asu.edu)

Takahashi and Yamanaka's 2006 and 2007 experiments showed that scientists can prompt adult body cells to dedifferentiate, or lose specialized characteristics, and behave similarly to embryonic stem cells (ESCs). (asu.edu)

We have generated fluorescent reporter hESC lines for HOXA9 and HOXA10 genes, and are preparing the constructs to target the earlier HOXA genes. (ca.gov)
In addition, we have generated hESC lines that express a fluorescent reporter from the regulatory elements of novel HSC genes, MLLT3 and HLF. (ca.gov)
Our studies also suggest that these hESC reporter lines will become valuable for investigators working on optimizing stem cell differentiation and culture for other tissues, as many of these reporter genes are not only restricted to HSC. (ca.gov)
Excitement over the ability of researchers to create this form of stem cell by inserting four genes into adult cells has thus far been tempered by the fact that the genes have been inserted using retroviruses, which have the potential to "turn on" cancer genes and trigger tumor growth. (harvard.edu)
After these experiments with somatic cells, Takahashi and Yamanaka hypothesized that there were common factors, genes in particular, which caused somatic cells to become pluripotent stem cells. (asu.edu)
In 2006, Takahashi and Yamanaka selected twenty-four candidate genes as factors that they hypothesized could possibly induce somatic cells to become pluripotent, and they began to test them one at a time. (asu.edu)
If one of the infected cells showed G418 resistance, then the scientists would know that one of the twenty-four genes influenced the cell to become an embryonic stem cell-like cell. (asu.edu)
Activation of muscle-specific genes in pigment, nerve, fat, liver, and fibroblast cell lines by forced expression of MyoD. (springer.com)
Through the use of human embryonic stem cells (hESCs), we can mimic brain development towards different regions of the human brain, and thereby investigate the effect of novel genes on neural differentiation. (lu.se)

The resulting cells maintained a normal karyotype and had high levels of pluripotency markers. (nih.gov)

On the strength of these findings, we have made KOLF2.1J and its gene-edited derivative clones readily accessible to promote the standardization required for large-scale collaborative science in the stem cell field. (ca.gov)
Each gene was inserted near the mouse Fbx15 gene, a gene that embryonic stem cells express during development in mice. (asu.edu)
Through this RFA, we encourage individual and collaborative applications that study and compare gene expression at the tissue and single-cell level. (sfari.org)
Immunogenicity is reduced by removing the major histocompatibility complex molecules from the stem cells with gene editing, and tolerance induced via "immune editing. (medscape.com)
Current efforts focus on identifying the optimal starting cell population for neural conversion, identification of gene combinations that yield subtype specificity and analysis of function and integration of the reprogrammed neurons. (lu.se)
However, in some of them, the disorder develops because of a germ cell mutation in their parents (eg, an autosomal dominant gene in a phenotypically normal parent). (msdmanuals.com)

In other words, dead people can yield living cells that can be converted into any cell or tissue in the body. (livescience.com)
Mature cells can be made or induced to become immature cells, known as pluripotent stem cells , which have the ability to become any tissue in the body and potentially can replace cells destroyed by disease or injury. (livescience.com)
Fibroblasts are the most common cells of connective tissue in animals, and they synthesize the extracellular matrix, the complex scaffolding between cells. (livescience.com)
By examining the process of stem cells turning into cells in bones, heart muscle, nerves, and other organs and tissue, researchers can better understand how diseases and conditions develop. (globenewswire.com)
Stem cells have the potential to be grown to become new tissue for use in transplant and regenerative medicine. (globenewswire.com)
New areas of study include the effectiveness of using human stem cells that have been programmed into tissue-specific cells to test new drugs. (globenewswire.com)
Cell and tissue banking applications will account for the leading share of the market by 2020. (globenewswire.com)
Thus far, a large number of lines have been derived from skin tissue or whole blood (from both healthy and diseased subjects), and each line has been characterized via at least a subset of eight different assays, including exome-seq, RNA-seq, cellular phenotyping, proteomics analyses, and others. (genengnews.com)
The use of the pluripotent and/or self-renewing qualities of stem cells is believed to have therapeutic benefits for the regeneration of tissue in humans. (citizendium.org)
Drive technology development to create novel organoid and hybrid-cell models to investigate the role of cellular heterogeneity in human tissue and organ aging. (salk.edu)

Reprogramming cancer cells to pluripotency by induced pluripotent stem cell (iPSC) technology, which can be then programmed back to their original cellular state, allows for studying the dynamic events in the course of the disease progression. (elsevierpure.com)
Herein, we describe how to create and maintain iPSC-like line from human PDAC by lentiviral transduction of reprogramming factors. (elsevierpure.com)
Yamanaka claimed that Gurdon's work in reprogramming mature cells in frogs ( Xenopus ) in 1962 influenced his own work in reprogramming differentiated cells. (asu.edu)
Successfully reprogramming induced pluripotent stem cells so they behave like the cells they are meant to replace means that samples of the mimicked cells must be present for comparison. (livescience.com)
Unlike iPSC-based reprogramming processes that can erase epigenetic and transcriptional signatures of aging, direct-conversion approaches can better maintain molecular signatures of aging in resultant cell types. (salk.edu)
Stem cell-like transcriptional reprogramming mediates metastatic resistance to mTOR inhibition. (rochester.edu)
It provides a framework to explore strategies of reprogramming a cell from a differentiated state to a stem cell state through directed perturbations. (lu.se)
Such an approach is highly relevant to regenerative medicine since it allows for a rapid search over the host of possibilities for reprogramming to a stem cell state. (lu.se)
Recent breakthroughs in reprogramming differentiated cells loops. (lu.se)
A computational model of master regulators that are required for successful reprogramming the dynamics of this core network has revealed that it functions as of a differentiated cell into a cell exhibiting stem cell like a bistable switch, which in the on state, corresponds to all these properties. (lu.se)

Using these reporter lines to monitor the induction of HSC "stemless' program, we are making progress in optimizing differentiation conditions toward generating functional HSCs for transplantation. (ca.gov)
Transplantation of living nuclei from blastula cells into enucleated frogs' eggs. (springer.com)
Cross-species transplantation was possible without the rejection of the human embryonic stem cells by the mice's immune systems because the mice were genetically modified to suppress certain immune responses that would have interfered with transplantation. (citizendium.org)
We monitor our cells after transplantation using standard in vivo assessments as listed above. (lu.se)

It has previously been believed that the viruses carrying the four essential transcription factors had to be integrated into the genome of the target cell in order for adult cells to be reprogrammed into pluripotent stem cells, "but we've shown that you don't need integration of the virus into the genome to produce iPS cells," Hochedlinger said. (harvard.edu)
Hochedlinger and colleagues Matthias Stadtfeld - the study's lead author - Masaki Nagaya, Jochen Utikal, and Gordon Weir, head of HSCI's Diabetes Program, have used the new technique to create iPS cells from mouse skin cells, and mouse fetal and adult liver cells. (harvard.edu)
The developmental capacity of nuclei transplanted from keratinized skin cells of adult frogs. (springer.com)
Viable offspring derived from fetal and adult mammalian cells. (springer.com)
Obviously, it's a different cell type: it is neither a embryonic stem cell nor an adult stem cell. (scitizen.com)
Adult Stem Cells: The New Plastic Surgery? (scitizen.com)
Adult stem cells hold great promise in mitigating much of the ethical debate over embryonic stem cell use. (citizendium.org)

Identification of binding sites of EVI1 in mammalian cells. (rochester.edu)
Since 2015, more than 3,000 articles were published on studies using crispr /Cas9 genome editing, including more than 900 articles using the technology in mammalian cells (PubMed, accessed June 11, 2017). (brill.com)

A second press release [2] has indicated stem cell source, resulting in widespread excitement that the program is planning to move forward very rapidly, with all of the patients being enrolled in the Correspondence to: Roger A. Barker, John van Geest Centre first quarter of 2016 and interim results being shared for Brain Repair, Department of Clinical Neurosciences, Univer- in October 2016. (lu.se)

Totipotent cells have the capacity to differentiate to all cell types, including somatic cells, germ cells, and certain cells that exist outside the embryo and are important to fetal development that are termed extraembryonic cells. (citizendium.org)
By experimenting on germ cells, cytologist Nettie Maria Stevens collected evidence to support the connection between heredity and the sex of offspring. (asu.edu)
However, offspring can inherit the mutation only if germ cells are affected. (msdmanuals.com)

North America accounted for largest stem cell manufacturing market share in 2020. (globenewswire.com)
Stem cell research 2020 2 43 101708. (cdc.gov)

This led us to question whether excessive Ca V 3 channels, retained mistakenly in engineered human-induced pluripotent stem cell-derived islet (hiPSC-islet) cells, act as an obstacle to hiPSC-islet maturation. (mdpi.com)
If ultimately successful, both approaches would offer access to an unlimited number of implantable islets, representing a significant advance over the multiple human deceased donors that are currently required for islet-cell transplants to be undertaken in humans. (medscape.com)

To overcome this hurdle, we have generated fluorescent reporter hESC lines for a set of novel human HSC regulators that we have identified. (ca.gov)
To track the location and quantity of transplanted cells in recipient small animal model, we will generate hESC lines with in vivo reporter system that combines bioluminescent or PET imaging, and serum-based assay. (ca.gov)
shows with high certainty that every NT-hESC line derived here originated from the respective patient donor and that these lines were not the result of enucleation failures and subsequent parthenogenetic activation. (citizendium.org)
Single cell transcriptome analysis of day 27 hESC-derived hypothalamic neurons enabled us to identify specific hypothalamic cell types (e.g. (columbia.edu)
To understand the molecular neurophysiology of the obesity in PC1/3-deficient subjects, we generated PCSK1 deficient hESC lines with CRISPR or by knocking down PCSK1 with shRNA, and assessed the POMC processing in the hypothalamic ARC-like neurons made from these lines. (columbia.edu)

The mutation causing sickle cell disease confers resistance to malaria. (msdmanuals.com)
However, by causing symptoms and complications of sickle cell disease, the mutation also has harmful effects usually when present in the homozygous state. (msdmanuals.com)

You and your colleagues found amniotic fluid-derived (AFS) stem cells to have the potential to differentiate into muscle, bone, fat, blood vessel, nerve and liver cells. (scitizen.com)
Pluripotent cells may differentiate to cells of most types, and multipotent cells are capable only of differentiating to certain types within a group of cells that perform similar functions. (citizendium.org)

When and in what cell type mutations occur can explain certain abnormalities in inheritance patterns. (msdmanuals.com)

Komor, Badran and Liu, 2017 ) have opened up tremendous opportunities for the development of cell lines, especially those of human origin ( Tobita, Guzman-Lepe and de L'Hortet, 2015 ). (brill.com)

Next, Takahashi tried to insert into a fibroblast cell multiple retroviral factors instead of one at a time. (asu.edu)

In 2004, Yamanaka began working at Kyoto University as a professor, where he studied factors that help an organism fend off retroviruses, which are single-stranded RNA viruses that can incorporate their genetic material into the DNA of a host cell. (asu.edu)

We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)

Here, the stem cells are shown expressing various markers and differentiating into neurons. (livescience.com)
Criteria for diagnosis of MDS consist of anemia, thrombocytopenia, or neutropenia that persist for six months or longer, dysplasia greater than 10% in at least one bone marrow cell lineage, and MDS associated clonal cytogenetic abnormalities or molecular markers. (standardofcare.com)
These hypothalamic-like neurons accounted for over 90% of differentiated cells and exhibited transcriptional profiles characteristic of specific hypothalamic neurons (and explicitly lacking pituitary markers). (columbia.edu)

Now scientists have taken fibroblasts from the scalps and the brain linings of 146 human brain donors and grown induced pluripotent stem cells from them as well. (livescience.com)
5. 293T cells - must never be grown to confluency. (lu.se)
If successful, this proposed work could have an enormous impact on regenerative medicine, leading the way to rigorously tested universal-donor stem cells that could be grown and differentiated into very large numbers of cells, made widely available to all medical institutions and used on demand to treat patients with type 1 diabetes and a variety of degenerative diseases, he concluded. (medscape.com)

Phenotypes of retinal organoids differentiated from induced pluripotent stem cells of Nrl -GFP rd16 mice. (elifesciences.org)
Promotes differentiation of retinal pigment epithelium from mouse ES cells (Osakada et al. (stemcell.com)

Even more important, he said, thus far none of the mice carrying the new cell lines have shown any signs of developing tumors - and tumors were being frequently reported in mice carrying the cell lines created using retroviruses. (harvard.edu)
In 2006, Kazutoshi Takahashi and Shinya Yamanaka reprogrammed mice fibroblast cells, which can produce only other fibroblast cells, to become pluripotent stem cells, which have the capacity to produce many different types of cells. (asu.edu)
After sixteen weeks, the injured mice who received human stem cell injections experienced a significant improvement in the motor functions that had been impaired by their injuries. (citizendium.org)
Furthermore, the use of diphtheria toxin -- which is far more toxic to human cells than mouse cells -- to destroy the human neurons in the mice reversed the observed improvements in motor function. (citizendium.org)
Previous studies have found that DIM can improve type 2 diabetes by enhancing glucose uptake through the activation of insulin signaling in 3T3-L1 cells, and by lowering the plasma glucose levels in high-fat-diet-fed obese mice [13, 14]. (researchgate.net)

Anatomy36

Organisms9

Diseases5

Chemicals and Drugs39

Analytical, Diagnostic and Therapeutic Techniques and Equipment29

Phenomena and Processes45

Disciplines and Occupations3

Information Science3

IPSCs5

Differentiation of human pluripotent stem1

Producing induced1

Neurons derived from human1

Pancreatic6

Embryos8

IPSC lines12

Progenitor Cells3

Endoderm3

Biology8

Feeder-free conditions1

Hematopoietic stem4

Vitro9

Therapies11

Characterization3

Peripheral blood mononuclear cells1

Neuronal2

Ductal2

Morphology2

Parkinson's6

Self-renewal3

Establishment2

Blastocyst2

Proliferation2

Somatic cell nuc1

Embryonic stem cell prope1

ESCs1

Genes9

Pluripotency markers1

Gene6

Tissue10

Reprogramming10

Transplantation4

Adult7

Mammalian cells2

20161

Germ cells3

20202

Islet2

HESC5

Mutation2

Differentiate2

Inheritance1

20171

Fibroblast cell1

Organism1

Lentiviral transduction1

Markers3

Grown3

Retinal2

Mice5