Pluripotent blood stem cells. Medical search. Frequent questions

Derivation and differentiation of haploid human embryonic stem cells. (nature.com)
Here, we employed a human pancreatic differentiation platform complemented with an shRNA screen in human pluripotent stem cells (PSCs) to identify potential drivers of early endoderm and pancreatic development. (mdpi.com)
Conventional methods for hematopoietic differentiation of human pluripotent stem cells (hPSC) rely on embryoid body (EB) formation and/or co-culture with xenogeneic cell lines. (biolamina.com)
In this study, the authors describe the development of a scalable, serum-free, xeno-free, and chemically defined microcarrier-based platform using human recombinant laminin-521 as an extracellular matrix (ECM) for hPSC expansion, EB formation, and subsequently hematopoietic differentiation of hPSC to red blood cells (RBS). (biolamina.com)
In vitro models mimicking the human BBB are of great interest, and advances in induced pluripotent stem cell (iPSC) technologies and the availability of reproducible differentiation protocols have facilitated progress. (ox.ac.uk)
In this study, we present the efficient differentiation of three different wild-type iPSC lines into brain microvascular endothelial cells (BMECs). (ox.ac.uk)
As with other types of stem cells, scientists are learning how to promote the differentiation of specific cell types from iPSCs efficiently, so that the needed cell types can be produced in adequate quantities. (jove.com)
Systemic transplantation of wild-type hematopoietic stem and progenitor cells (green) led to their differentiation into microglia-like cells (red), which reduced the amount of beta amyloid plaques (magenta) in the brain. (scitechdaily.com)
This change in gene activity blocks the maturation (differentiation) of blood cells and leads to the production of abnormal, immature white blood cells called myeloid blasts. (medlineplus.gov)
Decades of efforts have attempted to differentiate the pluripotent stem cells (PSCs) into truly functional hematopoietic stem cells (HSCs), yet the problems of low differentiation efficiency in vitro and poor hematopoiesis reconstitution in vivo still exist, mainly attributing to the lack of solid, reproduced, or pursued differentiation system. (biomedcentral.com)
In this study, we established an in vitro differentiation system yielding in vivo hematopoietic reconstitution hematopoietic cells from mouse PSCs through a 3D induction system followed by coculture with OP9 stromal cells. (biomedcentral.com)
We interestingly found that our 3D+OP9 system enriched a higher percentage of CD201 + c-kit + cells that showed more similar HSC-like features such as transcriptome level and CFU formation ability than CD201 - c-kit + cells, which have not been reported in the field of mouse PSCs hematopoietic differentiation. (biomedcentral.com)
Altogether, our findings lay a foundation for improving the efficiency of hematopoietic differentiation and generating in vivo functional HSC-like cells from mouse PSCs for clinical application. (biomedcentral.com)
Globally, miRNAs directly repress the pluripotent core factors during differentiation state. (biomedcentral.com)
While miRNAs directly repress pluripotent core factors to facilitate differentiation during the differentiation state, they also help stem cells to maintain pluripotency by activating pluripotent cores through directly repressing DNA methylation systems and primarily inhibiting development in the pluripotent state. (biomedcentral.com)
On the other hand, miRNAs like let-7 induce stem cell differentiation [ 11 ]. (biomedcentral.com)
This present study employed systems physical network approaches [ 17 ] and constructed a comprehensive and unbiased map of genome-wide interactions between miRNAs and their targets to investigate the global basis of miRNA roles in pluripotent stem cells, from the pluripotent self-renewal state to the differentiation state. (biomedcentral.com)
The 2024 edition of the Stem Cell Stars seminar series will explore diverse topics from the dynamic processes of cell differentiation to the latest advances in stem cell engineering. (lu.se)
Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)
Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)
The basic and common immunologic defect in common variable immunodeficiency is a failure of B-lymphocyte differentiation into plasma cells that produce the various immunoglobulin (Ig) isotypes. (medscape.com)
These studies described failure of B-cell differentiation because of altered B-cell surface-molecule expression. (medscape.com)

Pluripotent stem cells hold promise in the field of regenerative medicine. (wikipedia.org)
Cells from the blood samples are reprogrammed into clinical-grade human induced pluripotent stem cells, which can be distributed to recipients for regenerative medicine. (wjgnet.com)
Non-reproducible treatment outcomes or even absence of treatment effects in comparison to control groups challenges the potential of these cells for routine application both in tissue engineering and in regenerative medicine. (frontiersin.org)
During more recent years, his reseach has been conducted in the field of regenerative medicine, focussed on antigen expression and immune recognition of human embryonic stem cells and bioartificial heart valves, the latter tissues being of animal origin. (gu.se)
SAN DIEGO , Aug. 8, 2014 /PRNewswire/ -- ViaCyte, Inc., a privately held regenerative medicine company developing a stem cell-derived islet replacement therapy for the treatment of diabetes, announced today that it has completed a private equity financing transaction, providing the Company with $5.4 million through the sale of Series C-1 Preferred Stock, together with warrants to purchase common stock. (biospace.com)
ViaCyte is a private regenerative medicine company focused on developing a novel cell therapy for the treatment of diabetes. (biospace.com)
Stem cell-based regenerative medicine shows much promise. (selectbiosciences.com)
Stem cells are characterized by their ability to self-renew and differentiate into specialized cell types, making them ideal for use in regenerative medicine. (arthritistreatmentlab.com)
A new seminar series featuring some of the brightest minds in stem cell science and regenerative medicine from around the world. (lu.se)

This study systematically analyzes multiple-omics data and builds a systems physical network including genome-wide interactions between miRNAs and their targets to reveal the systems mechanism of miRNA functions in mouse pluripotent stem cells. (biomedcentral.com)

Human pluripotent stem cells, with their ability to proliferate indefinitely and to differentiate into virtually all cell types of the human body, provide a novel resource to study human development and to implement relevant disease models. (mdpi.com)
We found that: (1) most events within the 'VSEL' flow-cytometry gate had little DNA and the cells corresponding to these events (2) could not form spheres, (3) did not express Oct4, and (4) could not differentiate into blood cells. (ca.gov)
Eventually, the cells begin to differentiate, taking on a certain function in a part of the body. (healthline.com)
Adult stem cells can't differentiate into as many other types of cells as embryonic stem cells can. (healthline.com)
They can differentiate into all types of specialized cells in the body. (healthline.com)
The breakthrough has created a way to "de-differentiate" the stem cells. (healthline.com)
However, scientists have found a way to reprogram these mature cells so that they "de-differentiate" and return to an unspecialized, proliferative state. (jove.com)
Mishra and her colleagues performed systemic transplantations of healthy wild-type hematopoietic stem and progenitor cells into Alzheimer's mice and found that the transplanted cells did differentiate into microglia-like cells in the brain. (scitechdaily.com)
Mesenchymal stem/stromal cells (MSCs) have the capacity to differentiate into cells and tissues of one germ layer, here the mesodermal lineage, and are consequently multipotent. (frontiersin.org)
In the early embryo, embryonic stem cells give rise to all of the cell types in the organism, including adult stem cells, which continually replace cells in the adult tissues that die or differentiate into more mature cells like red blood cells. (sciencedaily.com)
You and your colleagues found amniotic fluid-derived (AFS) stem cells to have the potential to differentiate into muscle, bone, fat, blood vessel, nerve and liver cells. (scitizen.com)
Like stem cells, progenitor cells have a capacity to differentiate into a specific type of cell. (phys.org)
In contrast to stem cells, however, they are already far more specific: they are pushed to differentiate into their "target" cell. (phys.org)
When implanted under the skin, the PEC-01 cells are designed to mature and further differentiate into insulin-producing beta and other endocrine cells that regulate blood glucose in a manner similar or identical to the islets that normally comprise the endocrine pancreas. (biospace.com)
Stem cells have the ability to differentiate into various types of cells, including those needed for cartilage repair and regeneration. (arthritistreatmentlab.com)
Reduce Inflammation: Stem cells can differentiate into cells that produce anti-inflammatory cytokines, reducing inflammation in the affected joints. (arthritistreatmentlab.com)
Promote Tissue Repair: Stem cells can differentiate into chondrocytes, the cells that make up cartilage, promoting cartilage repair and regeneration. (arthritistreatmentlab.com)

Although hematopoietic stem and progenitor cells (HSPCs) enable autologous treatment of blood disorders, de novo generation of hematopoietic cells from pluripotent stem cells still compromises the unlimited high yield and rich hematopoiesis engraftment potential [ 2 ]. (biomedcentral.com)

Induced pluripotent stem cells (also known as iPS cells or iPSCs) are a type of pluripotent stem cell that can be generated directly from a somatic cell. (wikipedia.org)
The iPSC technology was pioneered by Shinya Yamanaka and Kazutoshi Takahashi in Kyoto, Japan, who together showed in 2006 that the introduction of four specific genes (named Myc, Oct3/4, Sox2 and Klf4), collectively known as Yamanaka factors, encoding transcription factors could convert somatic cells into pluripotent stem cells. (wikipedia.org)
Patient-matched embryonic stem cell lines can now be derived using somatic cell nuclear transfer (SCNT). (wikipedia.org)
Several human postnatal somatic cell types have been successfully reprogrammed to induced pluripotent stem cells (iPSCs). (johnshopkins.edu)
Blood cells from adults, especially nonlymphoid cells that are replenished frequently from intermittently activated blood stem cells, are short-lived in vivo and may contain less somatic mutations than skin fibroblasts, which are more exposed to environmental mutagens over time. (johnshopkins.edu)
In 2007, a team at Kyoto University created pluripotent stem cells from adult human somatic cells (4). (the-scientist.com)
This presentation will describe the current status of strategies to generate induced pluripotent stem (iPS) cells from mouse and human somatic cells for patient-specific disease modeling and cell therapeutic applications. (selectbiosciences.com)
In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)

Our work shows that hematopoietic stem and progenitor cell transplantation has the potential to prevent complications from Alzheimer's and could be a promising therapeutic avenue for this disease. (scitechdaily.com)
Altogether, the transplantation of healthy hematopoietic stem and progenitor cells led to enhanced microglia health, which in turn protected against multiple levels of Alzheimer's pathology. (scitechdaily.com)
The findings, which could someday have implications for infertility, contraception, and stem cell transplantation therapy, will be published in the June issue of Nature Genetics. (sciencedaily.com)
These treatments use stem cells from umbilical cord blood or bone marrow or haematopoietic stem cell transplantation. (centenary.org.au)
Human pluripotent stem cells harbor the potential to provide an inexhaustible supply of donor cells or tissues or organs for transplantation," Wu wrote in an email. (the-scientist.com)
The in vivo hematopoietic reconstitution potential of c-kit + cells derived from the mouse PSCs was evaluated via m-NSG transplantation assay. (biomedcentral.com)
Stem cell transplantation is the removal of stem cells (undifferentiated cells) from a healthy person and their injection into someone who has a serious blood disorder. (msdmanuals.com)
Overview of Transplantation Transplantation is the removal of living, functioning cells, tissues, or organs from the body and then their transfer back into the same body or into a different body. (msdmanuals.com)
But researchers think that these stem cells have the most potential for producing different kinds of cells and for surviving after transplantation. (msdmanuals.com)
These stem cells are most often used for transplantation. (msdmanuals.com)
Recent news of an impending clinical cell transplantation trial in Parkinson's disease using parthenogenetic stem cells as a source of donor tissue have raised hopes in the patient community and sparked discussion in the research community. (lu.se)
Based on discussions held by a global collaborative initiative on translation of stem cell therapy in Parkinson's disease, we have identified a set of key questions that we believe should be addressed ahead of every clinical stem cell-based transplantation trial in this disorder. (lu.se)
Accumulated data indicate that hematopoietic stem cell transplantation may be effective under optimal conditions in preventing the progression of central nervous system symptoms in neuronopathic forms of lysosomal storage diseases (such as Krabbe disease), including some of the mucopolysaccharidoses, oligosaccharidoses, sphingolipidoses, and lipidoses as well as peroxisome disorders such as X-linked adrenoleukodystrophy. (medscape.com)
Although longitudinal natural history data are limited, published guidelines are available to assist with decisions related to the pursuit of transplantation and whether to use bone marrow or umbilical cord blood-derived cells. (medscape.com)
Some evidence indicates that at least in certain disorders, combination ERT and hematopoietic stem cell transplantation together might be superior to hematopoietic stem cell transplantation alone in patients who are appropriate candidates. (medscape.com)
The availability of both ERT and hematopoietic stem cell transplantation has prompted ongoing consideration of newborn screening efforts to diagnose lysosomal storage diseases. (medscape.com)
Accumulated data indicate that hematopoietic stem cell transplantation may be effective under optimal conditions in preventing the progression of central nervous system symptoms in neuronopathic forms of lysosomal storage diseases, including some of the mucopolysaccharidoses, oligosaccharidoses, sphingolipidoses, and lipidoses. (medscape.com)

These new types of cells are called induced pluripotent stem cells (iPSCs). (healthline.com)
These cells are also pluripotent like embryonic stem cells-able to produce all cell types-and are therefore called induced pluripotent stem cells (iPSCs). (jove.com)

In most cases, clinically relevant cell numbers for MSC-based therapies can be only obtained by in vitro expansion of isolated cells. (frontiersin.org)
Some researchers are attempting to use stem cells to bioengineer human organs in the lab in vitro , rather than inside another species (5). (the-scientist.com)
Flow cytometry analysis, RNA-seq, and cell cycle analysis were used to detect the in vitro hematopoietic ability of endothelial protein C receptor (EPCR, CD201) cells generated in our induction system. (biomedcentral.com)
Further, we innovatively demonstrated that notch signaling pathway is responsible for in vitro CD201 + hematopoietic cell induction from mouse PSCs. (biomedcentral.com)
We are working to generate red blood cells in vitro from pluripotent stem cells, however the extremely large cell numbers involved pose unique challenges to culture technology and scale-up. (selectbiosciences.com)
In vitro experiments using NV virus-like particles (VLPs) directly showed NV VLP attachment to HBGA, resulting in VLP internalization into the cell ( 19 ). (cdc.gov)
From these critical studies, putative NV receptors were identified, and thus it was hypothesized that a successful in vitro cell culture system would most likely possess these receptors to support NV replication. (cdc.gov)

The fibroblasts were engineered so that any cells reactivating the ESC-specific gene, Fbx15, could be isolated using antibiotic selection. (wikipedia.org)
3) did not express a gene common to pluripotent cells (i.e. (ca.gov)
The advantages of briefly cultured blood MNCs may be due to favorable epigenetic profiles and gene expression patterns. (johnshopkins.edu)
A rearrangement (translocation) of genetic material involving the RUNX1 gene is found in approximately 7 percent of individuals with a form of blood cancer known as acute myeloid leukemia (AML). (medlineplus.gov)
Translocations and other types of mutations involving the RUNX1 gene have been associated with different types of leukemia and related blood disorders, including acute lymphoblastic leukemia (ALL), chronic myelomonocytic leukemia (CMML), familial platelet disorder with predisposition to acute myeloid leukemia, and myelodysplastic syndromes (MDS). (medlineplus.gov)
Researchers have identified the first gene linked to the productivity of the stem cells that produce sperm in mammals. (sciencedaily.com)
As scientific understanding of stem cells, gene editing, and organism development improved, Garry felt that her career path was clear. (the-scientist.com)
However, these recent studies have mostly focused on individual gene functions in stem cells although genome-wide data might be employed, and the conclusions drawn from these current studies are unavoidably biased on genes selected by these studies. (biomedcentral.com)
This complexity of miRNA interaction network presents a challenge for conventional approaches like gene-knockout to unbiasedly capture the real mechanisms of miRNA functions in stem cells. (biomedcentral.com)
Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)

Blood Adv. 2023 Sep 20. (bu.edu)

The most well-known type of pluripotent stem cell is the embryonic stem cell. (wikipedia.org)
They hypothesized that genes important to embryonic stem cell (ESC) function might be able to induce an embryonic state in adult cells. (wikipedia.org)
Obviously, it's a different cell type: it is neither a embryonic stem cell nor an adult stem cell. (scitizen.com)
Before that, Dr. Weiss was the Associate Director for The Human Embryonic Stem Cell Core Facility at The Children's Hospital of Philadelphia. (uthsc.edu)
ViaCyte's VC-01 product candidate consists of pancreatic progenitor cells, called PEC-01 cells, which are derived from a proprietary human embryonic stem cell line, encapsulated in ViaCyte's Encaptra® device. (biospace.com)
We discuss these properties with examples both from the hematopoietic and embryonic stem cell (ESC) systems. (lu.se)

The terms "progenitor cell" and "stem cell" are sometimes equated. (phys.org)
Part of the Progenitor Cell Biology Consortium, the scientists are working to make sure this growing area of medical research is grounded in safe and sound science. (neurosciencenews.com)

Published reports have indicated that a cell known as the "very small embryonic-like stem cell" (VSEL) can be isolated from bone marrow and is pluripotent-i.e., has the ability to generate all types of tissue in the body. (ca.gov)
Very small embryonic-like stem cells (VSELs) isolated from bone marrow (BM) have been reported to be pluripotent. (ca.gov)
For example, hematopoietic stem cells are a type of adult stem cell found in bone marrow. (healthline.com)
Doctors have been performing stem cell transplants, also known as bone marrow transplants, for decades using hematopoietic stem cells in order to treat certain types of cancer. (healthline.com)
On the other hand, over-estimation of chronological age in bone marrow failure syndromes is indicative for exhaustion of the hematopoietic cell pool. (nih.gov)
The study focused on hematopoietic stem cells (HSCs), the cells in our bone marrow that produce new blood and immune cells throughout our lives. (scitechdaily.com)
The adult cells are found in small numbers in most tissues, such as bone marrow or fat. (centenary.org.au)
In addition, CD4 has also been reported to be detectable on pluripotent hematopoietic stem cells, bone marrow myeloid and B-lymphocyte precursors, intrathymic lymphoid precursors, and a subset of splenic dendritic cells. (bdbiosciences.com)
This can range from the relatively pedestrian, such as a person who received a bone marrow transplant, to creatures that seem more at home in science fiction, such as animals containing cells or tissues belonging to other species. (the-scientist.com)
Pluripotency can be tested to determine if iPSC lines are able to form what are called teratomas - benign tumors made up of different cell types (teeth, bone, brain, etc. (neurosciencenews.com)
It starts in MYELOID CELLS of the bone marrow, invades the blood and then other organs. (bvsalud.org)
Blood is preferred to bone marrow as a source because the procedure is less invasive and the number (count) of blood cells returns to normal more quickly. (msdmanuals.com)
The bone marrow and blood of children and adults contain stem cells. (msdmanuals.com)

Since stem cells have the ability to turn into various other types of cells, scientists believe that they can be useful for treating and understanding diseases. (healthline.com)
Scientists have recently discovered how to turn adult stem cells into pluripotent stem cells. (healthline.com)
To create iPSCs, scientists genetically reprogram the adult stem cells so they behave like embryonic stem cells. (healthline.com)
Scientists are hoping that the cells can be made from someone's own skin to treat a disease. (healthline.com)
Scientists working in basic, translational, and clinical cancer metabolism research are invited to join the Academy in New York on April 17th to discuss the intersection between cell signaling and metabolism. (nyas.org)
A recent study conducted by scientists at the University of California San Diego suggests that stem cell transplants could be a promising therapeutic against Alzheimer's. (scitechdaily.com)
Scientists at UC San Diego have discovered that stem cells' unique method of discarding misfolded proteins could hold the key to preserving long-term health and avoiding illness. (scitechdaily.com)
According to a study recently published in the journal Cell Stem Cell , scientists at the University of California San Diego School of Medicine discovered that blood stem cells employ a surprising technique to eliminate their misfolded proteins. (scitechdaily.com)
UC San Diego scientists found misfolded proteins were aggregated and caged into a single area (green) within stem cells before being disposed of. (scitechdaily.com)
Scientists have found a way to possibly avoid using embryonic stem. (scitizen.com)
In 1995, he was one of the leading scientists conducting the clinical trial in which pig kidneys were connected extracorporeally to the blood circulation of volunteer dialysis patients. (gu.se)
Induced pluripotent stem cells, or 'iPS cells', are stem cells that scientists make in the laboratory. (yourgenome.org)
Scientists are using induced pluripotent stem cells to produce new RPE cells in the lab that can then be put into a patient's eye to replace the damaged cells. (yourgenome.org)
Other scientists, including Jun Wu, a stem cell biologist at the University of Texas Southwestern Medical Center, are also studying chimeras with the ultimate goal of one day being able to grow enough human organs to meet the enormous need for transplants, potentially saving hundreds of thousands of lives. (the-scientist.com)
For decades scientists have been trying to reproduce cell culture environment for the closest mimicry of the natural cellular "embedding" within a living organism. (selectbiosciences.com)
As the promise of using regenerative stem cell therapies draws closer, a consortium of biomedical scientists reports about 30 percent of induced pluripotent stem cells they analyzed from 10 research institutions were genetically unstable and not safe for clinical use. (neurosciencenews.com)
It gives scientists open access to data from the study to support their own research into potential iPSC-based stem cell therapies. (neurosciencenews.com)
Immerse yourself in groundbreaking insights and discoveries, engage in captivating discussions, gain fresh perspectives, and network with fellow scientists and Stem Cell Center members during our post-seminar mingling sessions. (lu.se)
Scientists are developing ways of enabling (inducing) other cells (such as a blood or skin cell) to act as stem cells. (msdmanuals.com)

The field of stem cell research has advanced significantly with the use of adult or pluripotent stem cells (PSCs). (centenary.org.au)
In fact, only using the OP9 co-culture system combined with hematopoietic related cytokines, we hardly obtained the in vivo functional reconstitution hematopoietic cells from PSCs [ 9 ]. (biomedcentral.com)

These unlimited supplies of autologous cells could be used to generate transplants without the risk of immune rejection. (wikipedia.org)
and (4) could not generate one type of tissue previously reported: i.e., blood cells. (ca.gov)
Skin stem cells can be used to generate new skin for people with severe burns. (yourgenome.org)
This natural ability to generate new cell types is unique to the stem cell. (centenary.org.au)
Being pluripotent means iPSCs can generate cells from each of the three basic germ cell lines that form the body - endoderm (gut region), ectoderm (epidermis, nerve tissue, etc.) and mesoderm (muscles, blood cells, etc. (neurosciencenews.com)

Our CTS products have been used in over 200 clinical trials as well as in commercially approved cell therapies including FDA-approved CAR-T therapies and the first FDA-approved therapeutic cancer vaccine. (thermofisher.com)
3 Today, serologic and molecular techniques, along with laboratory information systems and electronic health records, contribute to precise blood product management and personalized transfusion therapies, particularly benefiting complex patients with sickle cell disease, thalassemia, and other diseases requiring chronic transfusion support. (cap.org)
In this mini review, we will discuss point by point possible pitfalls in the production of human MSCs for cell therapies, without consideration of material-based applications. (frontiersin.org)
In addition to these already established stem cell therapies, there is ongoing research and development of new stem cell therapies, which are currently being investigated in clinical trials involving people. (centenary.org.au)
It was very surprising to us the high number of unstable cell lines identified in the study, which highlights the importance of setting safety standards for stem cell therapies," said Carolyn Lutzko, PhD, senior author and director of translational development in the Translational Core Laboratories at Cincinnati Children's Hospital Medical Center. (neurosciencenews.com)
A good number of the cell lines we studied met quality standards, although the unexpected number of lines that did not meet these standards could not be used for clinical therapies. (neurosciencenews.com)
Are Stem Cell-Based Therapies for Parkinson's Disease Ready for the Clinic in 2016? (lu.se)
Stem cell-based therapies for Parkinson's dis- ogy company International Stem Cell Corporation ease (PD) are rapidly moving towards clinical trials. (lu.se)

Leeb, M. & Wutz, A. Derivation of haploid embryonic stem cells from mouse embryos. (nature.com)
citation needed] Since iPSCs can be derived directly from adult tissues, they not only bypass the need for embryos, but can be made in a patient-matched manner, which means that each individual could have their own pluripotent stem cell line. (wikipedia.org)
Embryonic stem cells come from human embryos that are three to five days old. (healthline.com)
Embryonic stem cells are derived from embryos and have the ability to become any type of cell in the body, while adult stem cells are found in various tissues and can develop into different cell types within that tissue. (arthritistreatmentlab.com)
Stem cells can be obtained from the embryos that are not used. (msdmanuals.com)
Because the embryos then lose the ability to grow into a complete human being, the use of stem cells from embryos is controversial. (msdmanuals.com)

Wutz, A. Haploid mouse embryonic stem cells: rapid genetic screening and germline transmission. (nature.com)
Genetic modification and screening in rat using haploid embryonic stem cells. (nature.com)
These cells have been successfully used to treat children with blood cancers, such as leukemia, and certain genetic blood disorders. (healthline.com)
Cells, tissues and organs can sometimes be permanently damaged or lost by disease, injury and genetic conditions . (yourgenome.org)
In our image, induced pluripotent stem cells can be seen, derived from patients, that replicate unique heart cell behaviours in relation to genetic heart disease. (centenary.org.au)
It also is vital that - prior to being instructed to become a specific cell type - iPSC lines continuously renew and expand in a blank slate form without the introduction of genetic errors. (neurosciencenews.com)
The different methods included a variety of reprogramming genes, vectors (engineered viruses that deliver genetic material to cells), or the use of plasmids (small DNA molecules that can deliver reprogramming genes). (neurosciencenews.com)
Clonal hematopoetic disorder caused by an acquired genetic defect in PLURIPOTENT STEM CELLS. (bvsalud.org)

The resulting β-amyloid build-up also puts stress on other brain cells, including endothelial cells that affect blood flow to the brain. (scitechdaily.com)
Those that had received the stem cell therapy had less cortical expression of genes associated with diseased microglia, and less hippocampal expression of genes associated with diseased endothelial cells. (scitechdaily.com)
Endothelial cells (ECs) line blood vessels and can serve as specialized vascular niches for hematopoietic stem and progenitor cells (HSPCs), a special environment where HSPCs reside and self-renew. (phys.org)
Endothelial cell α-globin and its molecular chaperone α-hemoglobin-stabilizing protein regulate arteriolar contractility. (uthsc.edu)

To create iPSCs, mature cells, like skin fibroblasts, are taken from a patient and put into culture. (jove.com)
To create iPSCs, mature cells such as skin fibroblasts or blood cells from a person are grown in culture. (jove.com)
2008). Historically, this concept is highlighted by the experi- factors are key intrinsic regulators of these fate decisions and mental phenomenon of lineage reprogramming, for example, that fate choice involves modulating networks of transcription by the conversion of fibroblasts to muscles cells following trans- factors. (lu.se)

Adult stem cells supply new cells as an organism grows and to replace cells that get damaged. (yourgenome.org)
However, advances in stem cell research in the 2000s revolutionized the field, opening up new possibilities and new applications for multispecies organism research. (the-scientist.com)

Using our recently established haploid human pluripotent stem cells (hPSCs), we generated a genome-wide loss-of-function library targeting 18,166 protein-coding genes to define the essential genes in hPSCs. (nature.com)
hPSC-enriched essential genes mainly encode transcription factors and proteins related to cell-cycle and DNA-repair, revealing that a quarter of the nuclear factors are essential for normal growth. (nature.com)
Fig. 2: Analysis of cell-essential genes. (nature.com)
iPSCs are typically derived by introducing products of specific sets of pluripotency-associated genes, or "reprogramming factors", into a given cell type. (wikipedia.org)
Upon introduction of reprogramming factors, cells begin to form colonies that resemble pluripotent stem cells, which can be isolated based on their morphology, conditions that select for their growth, or through expression of surface markers or reporter genes. (wikipedia.org)
Methylation levels at three age-related CpGs--located in the genes ITGA2B, ASPA and PDE4C--were subsequently analyzed by bisulfite pyrosequencing of 151 blood samples. (nih.gov)
Then, typically, genes for transcription factors, are delivered by viral vectors into the cell nuclei, where they are incorporated into the genome. (jove.com)
The transcription factors then turn on genes that are expressed by embryonic stem cells. (jove.com)
Then, genes for multiple transcription factors are delivered into the cells using a viral vector, and the transcription factor proteins are expressed using the cell's machinery. (jove.com)
The transcription factors then turn on many other genes that are expressed by embryonic stem cells, returning the cells to an undifferentiated, proliferative, and pluripotent state. (jove.com)
The RUNX1 protein turns on (activates) genes that help control the development of blood cells (hematopoiesis). (medlineplus.gov)
While t(8;21) is important for leukemia development, a mutation in one or more additional genes is typically needed for the myeloid blasts to develop into cancerous leukemia cells. (medlineplus.gov)
The workings of stem cells within the testicles are not well understood in mammals, though a few genes have been linked to stem cell self-renewal in the fruit fly, which has a simpler anatomical structure. (sciencedaily.com)
This includes some cell lines contaminated with bacteria or carrying genes and mutations associated with cancer. (neurosciencenews.com)
The cells were generated with a variety of genes, methods and cells of origin, such as adult skin or infant cord blood cells. (neurosciencenews.com)
By triggering certain genes, researchers may be able to cause the stem cells to specialize and become the cells that need to be replaced. (msdmanuals.com)
One way to induce these cells is to inject them with material that affects their genes, a process called reprogramming. (msdmanuals.com)
explosion further, consider that a fictitious small genome with 2002) More recently and more dramatically, the potential for 260 genes would host the same number of combinations as cell state conversions is exemplified by the reprogramming of the number of atoms in the visible universe! (lu.se)

Stem cells have also been found in amniotic fluid. (healthline.com)
However, more research is needed to help understand the potential uses of amniotic fluid stem cells. (healthline.com)
He and his colleagues have identified a new stem cell found in amniotic fluid and placentas, named "AFS" cells. (scitizen.com)
Basically, we came to this conclusion by harvesting the cells from amniotic fluid making sure they were a true stem cell population and going through the reverse studies that need to be done to show that the cells truly are pluripotent. (scitizen.com)

Inducible Gata1 suppression expands megakaryocyte-erythroid progenitors from embryonic stem cells. (uthsc.edu)

The c-kit + cells from 3D self-assembling peptide induction system followed by the OP9 coculture system possessed apparently superiority in terms of in vivo repopulating activity than that of 3D induction system followed by the 0.1% gelatin culture. (biomedcentral.com)
Several possible mechanisms were considered for the induction of human leukemia, such as clastogenic damage to circulatory stem cells. (who.int)

Blood mononuclear cells (MNCs) offer several advantages compared with other cell types. (johnshopkins.edu)
Generation of three induced pluripotent stem cell lines (RAUi001-A, RAUi001-B and RAUi001-C) from peripheral blood mononuclear cells of a healthy Armenian individual. (nih.gov)

Cord blood stem cells are harvested from the umbilical cord after childbirth. (healthline.com)
They are easily isolated from umbilical cord blood (CB) or adult peripheral blood (PB), and can be used fresh or after freezing. (johnshopkins.edu)
Stem cells from umbilical cords are usually used only in children because umbilical cord blood does not contain enough stem cells to use in adults. (msdmanuals.com)
Stem cells can be obtained from the blood in the umbilical cord or placenta after a baby is born. (msdmanuals.com)

There are several types of stem cells that can be used for different purposes. (healthline.com)
What are the different types of stem cells? (centenary.org.au)
There are several different types of stem cells, including embryonic stem cells and adult stem cells. (arthritistreatmentlab.com)

How well the 58 iPSC lines met quality criteria depended on the origin of the reprogrammed cells (skin vs. blood, male vs. female) and specific reprogramming methods. (neurosciencenews.com)
It had been thought that poor quality iPSC lines could not be pluripotent, Lutzko said, although until the current study this theory had not been carefully tested because of the expense. (neurosciencenews.com)

Novel culture and cryopreservation systems for human pluripotent stem cells and neural stem cells will be presented and their advantages and applicability in the production of high quality advanced therapeutic products or functional screening tools for preclinical research discussed. (selectbiosciences.com)

Hematopoietic specification from human pluripotent stem cells: current advances and challenges toward de novo generation of hematopoietic stem cells. (bvsalud.org)

research - to help us understand the basic biology of how living things work and what happens in different types of cell during disease. (yourgenome.org)
My current research portfolio is a direct reflection of my evolution as a scientist in which my early studies of hematopoietic development led to the generation of useful tools and reagents as well as methodologies and insights that synergized into a potent platform in the emerging and rapidly expanding field of pluripotent stem cell biology. (bu.edu)
Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)

To varying degrees, these fates also extend to the Such state stability is required in stem and progenitor cells to immediate progeny of stem cells, known as progenitor or support self-renewal and maintenance of the uncommitted transit-amplifying cells. (lu.se)

These stem cells come from developed organs and tissues in the body. (healthline.com)
During embryonic development, the programmed death of certain cells plays an essential role in shaping organs and allowing proper growth. (phys.org)
If we understand stem cell development, we may be able to replicate this process to create new cells, tissues and organs . (yourgenome.org)
Stem cells can develop into any type of cell in the body, making them valuable for repairing damaged tissues and organs. (centenary.org.au)
Animals with human cells could provide donor organs or help us understand neuropsychiatric disorders. (the-scientist.com)
since the new organs would be made from their own cells, they wouldn't have to worry about organ rejection. (the-scientist.com)
Stem cells have the potential to regenerate damaged tissues and organs. (arthritistreatmentlab.com)

The Generation of Human γδT Cell-Derived Induced Pluripotent Stem Cells from Whole Peripheral Blood Mononuclear Cell Culture. (illumina.com)

We perform a comprehensive analysis of methylation profiles to narrow down 102 age-related CpG sites in blood. (nih.gov)
We demonstrate that most of these age-associated methylation changes are reversed in induced pluripotent stem cells (iPSCs). (nih.gov)
Furthermore, at the pluripotent state miRNAs predominately repress DNA methyltransferases, the core enzymes for DNA methylation. (biomedcentral.com)
The decreasing methylation repressed by miRNAs in turn activates the top miRNAs and pluripotent core factors, creating an active circuit system to modulate pluripotency. (biomedcentral.com)

All humans start out as only one cell. (healthline.com)
As humans continue their search for the fountain of youth, stem cells keep emerging as a key player in the quest for longevity. (scitechdaily.com)
For instance, the proteins through which ions flow in and out of heart cells can vary in both number and type between humans and other animals. (ieee.org)
Our results showed that inflamed and non-inflamed dental pulps neither from humans nor from rats presented mast cells. (bvsalud.org)
Since mast cells are active during inflammatory responses it becomes crucial to find out if these cells are actually present and play a role in dental pulp under normal and inflammatory conditions either in humans or in rats. (bvsalud.org)
Therefore, the purpose of the present work was, under the same experimental conditions, to identify mast cells in healthy and inflamed dental pulp from rats and humans using toluidine blue histochemistry and immunohistochemistry techniques. (bvsalud.org)

The researchers went on to show that another protein, OCT4, which functions to maintain the stem cells in the early embryo and in cultured embryonic stem cells, is also present in the adult germ stem cell. (sciencedaily.com)

Here, we show that we cannot find VSELs in mouse BM with any of the reported stem cell potentials, specifically for hematopoiesis. (ca.gov)

In addition, they show efficient terminal maturation and generation of mature enucleated RBCs using a co-culture system that comprised primary human mesenchymal stromal cells. (biolamina.com)
Despite almost 50 years of research and over 20 years of preclinical and clinical studies, the question of curative potential of mesenchymal stem/stromal cells (MSCs) is still widely discussed in the scientific community. (frontiersin.org)

The new cells could then be transplanted back into the patient to treat damage or disease with minimal risk of rejection, because they originated from the patient's own cells. (jove.com)

A study from UC San Diego indicates that transplants of hematopoietic stem cells can shield mice with Alzheimer's from memory decline, neuroinflammation, and accumulation of β-amyloid. (scitechdaily.com)
The Cherqui lab had already found success using similar stem cell transplants to treat mouse models of cystinosis, a lysosomal storage disease, and Friedreich's ataxia, a neurodegenerative disease. (scitechdaily.com)

Surprisingly, during the pluripotent state, the top important miRNAs do not directly regulate the pluripotent core factors as previously thought, but they only directly target the pluripotent signal pathways and directly repress developmental processes. (biomedcentral.com)
In terminally differentiated cell fate is coupled to appropriate regulation of the alternative cells, transcriptional networks must be stable and irreversible, pathways. (lu.se)

His research interests are to better understand the mechanisms of normal red blood cell development and use this information to treat common blood disorders, including sickle cell disease and betathalassemia. (uthsc.edu)

Advancing your pluripotent stem cell therapy research to clinical applications requires careful material selection as the quality of starting materials significantly impacts the properties of your final stem cell therapy product. (thermofisher.com)
At Thermo Fisher Scientific, we have developed Gibco Cell Therapy Systems (CTS) products across the pluripotent stem cell therapy workflow to ease the transition from discovery to commercial manufacturing. (thermofisher.com)
Proven use for a seamless transition from research to clinic - We offer an extensive selection of serum-free, xeno-free, or animal origin-free formulations with cell therapy specific intended use statements and complementary research use reagents. (thermofisher.com)
The success of the therapy stems from its effects on microglia, a type of immune cell in the brain. (scitechdaily.com)
How far away do you think we are from possibly using AFS stem cells for regenerative therapy? (scitizen.com)
therapy - to replace lost or damaged cells that our bodies can't replace naturally. (yourgenome.org)
This presentation describes the current state of the stem cells marketplace as it relates to their utilization for pharmaceutical screening as well as their development for cellular therapy. (selectbiosciences.com)
Details are provided about the quantitative market opportunity for various stem cell classes as well as the current state of the clinical trials space wherein stem cell-based therapeutics are being evaluated for therapy in various disease classes. (selectbiosciences.com)
Stem cell therapy has emerged as a breakthrough treatment option for arthritis. (arthritistreatmentlab.com)
Recent scientific studies and clinical trials have demonstrated the effectiveness of stem cell therapy in relieving pain and improving joint function in individuals with arthritis. (arthritistreatmentlab.com)
While each approach to stem cell therapy has its advantages and limitations, all have shown promising results in improving pain relief, joint function, and overall quality of life in individuals with arthritis. (arthritistreatmentlab.com)
In this article, we first provide a short history of cell therapy in Parkinson's disease and briefly describe the current state-of-art regarding human stem cell-derived dopamine neurons for use in any patient trial. (lu.se)

Thus, epigenetic changes upon aging seem to reflect biological aging of blood. (nih.gov)
I have developed an approach to science that utilizes multiple stem cell-based platforms to answer basic biological questions and combat human disease. (bu.edu)
Mast cells play an important role in a variety of biological processes and actively participate in the inflammatory response. (bvsalud.org)
Mast cells play an important role in a variety of biological processes including allergic reactions, atherosclerosis and inflammation 7,8 . (bvsalud.org)
The biological properties and clinical potential of stem cells elicit that are generated must not be unduly sensitive to small fluctu- continued scientific, commercial, and public interest. (lu.se)

Previous work showed that stem cells, including HSCs, synthesize proteins much slower than other cell types, prioritizing quality over quantity. (scitechdaily.com)

In particular, it plays an important role in development of hematopoietic stem cells, early blood cells that have the potential to develop into all types of mature blood cells such as white blood cells, red blood cells, and platelets. (medlineplus.gov)

What researchers are trying to do is unravel the mystery of the adult germ stem cells in male testicles, which are capable of producing an average of 1,500 sperm during every human heartbeat - or an average of 130 million sperm a day. (sciencedaily.com)
In the adult testicles, the germ stem cells can produce more germ stem cells, but can also produce daughter cells that go on to become sperm. (sciencedaily.com)
But researchers do not know how the germ stem cells "decide" whether to create other germ stem cells or commit to becoming sperm. (sciencedaily.com)
Buaas and Braun agreed that it sounded as if the mice were born with germ stem cells, the cells that produce sperm, but then lost their germ line early in puberty. (sciencedaily.com)
After a series of tests, the researchers concluded that because of the mutation, the cells were more likely to convert from germ stem cells into sperm, than to produce more germ stem cells to keep the process going. (sciencedaily.com)
Using a fluorescent antibody against the PLZF protein, the researchers were able to show directly that PLZF is expressed in the adult germ stem cells. (sciencedaily.com)
This important finding confirms earlier published studies suggesting that the adult germ stem cells are not far removed from embryonic stem cells. (sciencedaily.com)

Transcription factor RUNX1 promotes survival of acute myeloid leukemia cells. (medlineplus.gov)

An induced pluripotent stem cell (iPSC), is an adult stem cell which has been altered in a lab to behave more like embryonic stem cells. (centenary.org.au)

We then made sure the cells had the specific characteristics of stem cells that we now have come to realize as a scientific community. (scitizen.com)
Essentially, it's just an additional stem cell line that has some characteristics that will hopefully be useful clinically someday. (scitizen.com)
What we've identified has characteristics of both, so as such, it's going to have different characteristics and different implications than other cell types. (scitizen.com)
Researchers also compared the molecular and functional characteristics of iPSCs to human embryonic stem cells, which are used sparingly as a gold standard for benchmarking quality. (neurosciencenews.com)

Based on the widely cited Essential 8 Medium, Gibco CTS Essential 8 Medium is the first globally available human pluripotent stem cell (hPSC) culture medium with components not directly derived from animals. (thermofisher.com)

dubious - discuss] In his Nobel seminar, Yamanaka cited the earlier seminal work of Harold Weintraub on the role of myoblast determination protein 1 (MyoD) in reprogramming cell fate to a muscle lineage as an important precursor to the discovery of iPSCs. (wikipedia.org)
A key to keeping stem cells happy is maintaining protein homeostasis. (scitechdaily.com)
Still, some mistakes or protein damage are inevitable, so the researchers set out to understand how stem cells ensure these proteins are properly discarded. (scitechdaily.com)
When the researchers genetically disabled the aggrephagy pathway, the stem cells started to accumulate aggregated protein, which impaired their fitness, longevity and regenerative activity. (scitechdaily.com)
Mapping cellular response to destabilized transthyretin reveals cell- and amyloidogenic protein-specific signatures. (bu.edu)
The protein organization of a red blood cell. (bu.edu)

Researchers are investigating several approaches, such as enzymatic treatments of RBCs, the generation of RBCs from human induced pluripotent stem cells, and the development of artificial oxygen carriers, all with the goal of advancing universal blood. (cap.org)
In the study, recently published in Cell Reports , the researchers demonstrate that transplanting hematopoietic stem and progenitor cells was effective in rescuing multiple signs and symptoms of Alzheimer's in a mouse model of the disease. (scitechdaily.com)
The researchers then evaluated the animals' behavior and found that memory loss and neurocognitive impairment were completely prevented in mice that received the stem cell transplant. (scitechdaily.com)
Looking closer at the animals' brains, the researchers found that mice treated with healthy stem cells showed a significant reduction in β-amyloid plaques in their hippocampus and cortex. (scitechdaily.com)
18 Sep, 2007 12:13 pm Rice University researchers have engineered musculoskeletal cartilages with human embryonic stem cells, with the hope of eventually using the neotissue. (scitizen.com)
6 Jun, 2007 07:00 pm Researchers have reprogrammed skin cells into embryonic stem cells. (scitizen.com)
Researchers call stem cells 'pluripotent' cells, meaning that any given stem cell can become any of several types. (sciencedaily.com)
This makes them beneficial for researchers wanting to learn about various human cells and diseases in the laboratory. (centenary.org.au)
Researchers began to dream of a future in which a patients' own cells, perhaps from the blood or the skin, could be converted into these induced pluripotent stem cells and grown into whatever organ the patient needed. (the-scientist.com)
Teratomas, benign tumors containing the developing cells of different body parts, allowed researchers to see if the iPSCs could form the body's three basic germ cell lines - endoderm (gut region), ectoderm (epidermis, nerve tissue, etc.) and mesoderm (muscles, blood cells, etc. (neurosciencenews.com)
Instead, researchers at the University of California, Berkeley, hope that human heart cells grown on a chip could help replace animal testing. (ieee.org)
The heart cells in this device remained alive and functional for several weeks, during which time they could be used to test a variety of drugs, the researchers said in a press release . (ieee.org)
Researchers hope to use stem cells to repair or replace cells or tissues damaged or destroyed by such disorders as Parkinson disease, diabetes, and spinal injuries. (msdmanuals.com)

We're thrilled to have Chad Cowan, an associate professor at Harvard University who is at the Harvard Stem Cell Institute. (medscape.com)

Anatomy40

Organisms9

Diseases39

Chemicals and Drugs54

Analytical, Diagnostic and Therapeutic Techniques and Equipment71

Psychiatry and Psychology2

Phenomena and Processes38

Disciplines and Occupations4

Anthropology, Education, Sociology and Social Phenomena2

Technology, Industry, Agriculture1

Information Science3

Named Groups4

Health Care14

Differentiation23

Regenerative medicine9

Mouse pluripotent stem1

Differentiate17

Novo generation of hematopoietic cells1

Somatic8

Transplantation17

Called induced pluri2

Vitro7

Gene10

20231

Embryonic stem6

Progenitor cell2

Bone13

Scientists19

PSCs2

Generate5

Therapies8

Embryos6

Genetic8

Endothelial4

Fibroblasts3

Organism2

Genes18

Amniotic fluid4

Progenitors1

Induction2

Mononuclear cells2

Umbilical4

Types of stem cells3

IPSC lines2

Neural Stem1

Advances1

Biology3

Self-renewal1

Organs7

Peripheral1

Methylation4

Humans6

Early Embryo1

Hematopoiesis1

Stromal cells2

Patient's1

Transplants2

Pathways2

Sickle cell di1

Therapy12

Biological5

HSCs1

Platelets1

Germ stem cells7

Myeloid1

Adult stem1

Characteristics4

HPSC1

Protein6

Researchers13

Harvard Stem Cell Institute1