Pluripotency. Medical search. Frequent questions

The researchers also measured, in each well, the level of two proteins found to be pluripotency markers, OCT4 and cyclin B1. (eurekalert.org)
Although the mechanism of how these factors induced pluripotency in somatic cells is not completely understood, it is clear that the endogenous pluripotency genes OCT4 , SOX2 and NANOG were activated and, in turn, re-activated the autoregulatory loop that could maintain the pluripotent state independent of the transgenes. (biologists.com)
examined individual clones and found that endogenous pluripotency gene expression was activated and the promoters of OCT4 and NANOG , which are usually heavily methylated in differentiated cells, were demethylated in the newly converted iPS cells. (biologists.com)
The POU domain transcription factor OCT4 is a key regulator of pluripotency in the early mammalian embryo and is highly expressed in the inner cell mass of the blastocyst. (mpg.de)
Consistent with its essential role in maintaining pluripotency, Oct4 expression is rapidly downregulated during formation of the trophoblast lineage. (mpg.de)
Mouse somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) by defined factors known to regulate pluripotency, including Oct4, Sox2, Klf4, and c-Myc. (biomedcentral.com)
Differentiated somatic cells can be reprogrammed into a pluripotent-like state through four defined factors known to regulate pluripotency, including Oct4, Sox2, Klf4, and c-Myc (OSKM) [ 1 , 2 ]. (biomedcentral.com)
Induced pluripotent stem cells (iPSCs) are produced by introduction of the defined factors (Oct4, Sox2, Klf4, c-Myc: OSKM), and exhibit infinitive self-renewal and pluripotency. (biomedres.us)
Genome-wide ChIP-seq mapping showed that Brd4 and H4 acetylation are co-occupied in the genome, upstream of core pluripotency genes such as Oct4 and Nanog in ESCs and lineage-specific genes in embryoid bodies (EBs). (biomedcentral.com)

The developmental progression of embryonic stem cells into specialist cell types involves the activation of lineage-specific programs of gene expression and the silencing of genes involved in maintaining pluripotency. (ca.gov)
BRD4, an epigenetic regulator and transcription factor, binds to acetylated histones in super-enhancer regions and regulates the expression of essential pluripotency genes. (stemcellsportal.com)
Our findings uncover a dichotomous molecular function for the reprogramming factors, serving to reactivate endogenous pluripotency networks while simultaneously directly recruiting the Mbd3/NuRD repressor complex that potently restrains the reactivation of OSKM downstream target genes. (umn.edu)
In addition, we identified a number of differentially expressed genes that are involved in epigenetics, chromatin remodeling, apoptosis, and metabolism that may point to underlying molecular mechanisms that regulate pluripotency and trophoblast differentiation in humans. (mpg.de)
The Pluripotency Check PCR Primer Set consists of 16 forward and reverse primer mixes, based on nine genes expressed in pluripotent stem cells, plus two control genes. (takarabio.com)
Moreover, comparison of transcriptomes between hiPSCs cultured with CCL2 versus with bFGF, we found that CCL2 activates hypoxia related genes, suggesting that CCL2 enhanced pluripotency by inducing a hypoxic-like response.Further, we show that hiPSCs cultured with CCL2 can differentiate at a higher efficiency than culturing withjust bFGF and we show CCL2 can be used in feeder-free conditions [corrected]. (edu.sa)

The stem cell field witnessed a genuine breakthrough when a combination of solely four transcription factors ( Oct3 / 4 , Sox2 , Klf4 and c-Myc, OSKM ) proved enough to revert, in vitro, the differentiated status of a variety of cell types back to pluripotency, giving rise to so-called induced pluripotent stem (iPS) cells. (springer.com)
Sox2 modulation increases naïve pluripotency plasticity. (cam.ac.uk)

Particularly, by performing transcriptome analysis, we observed that several pluripotent transcriptional factors increase in knockout cell line, which explains the underlying loss of pluripotency in Sirt6-null iPS-like cell line. (biomedcentral.com)

This work marks an important step toward the understanding of the mechanisms that define and maintain pluripotency, and calls for the identification of the factors that participate in the establishment of transcriptional competence in pluripotent cells. (huji.ac.il)

Standard culture of human induced pluripotent stem cells (hiPSCs) requires basic Fibroblast Growth Factor (bFGF) to maintain the pluripotent state, whereas hiPSC more closely resemble epiblast stem cells than true naïve state ES which requires LIF to maintain pluripotency. (edu.sa)

Researchers at the Center for Cell-Based Therapy (CTC) in Ribeirão Preto, São Paulo State, have now investigated the functioning of 31 miRNAs observed in human ESCs and identified signaling pathways involved in both pluripotency and differentiation. (eurekalert.org)
After culture for three to four?days, automated image acquisition and analysis were performed to evaluate the effects on pluripotency maintenance and differentiation. (eurekalert.org)
In this Chapter, we dissect the proof-of-principle studies that demonstrated that cellular reprogramming to pluripotency can be induced in vivo, in spite of unfavorable pro-differentiation signals present within the tissues. (springer.com)
Induced pluripotency provides a tool to explore mechanisms underlying establishment, maintenance, and differentiation of naive pluripotent stem cells (nPSCs). (cam.ac.uk)
Expression of the pluripotency-associated OCT4A isoform was paralleled by a global rise in histone H3 methylation on Lys-4, a marker of active chromatin, and coincided with peak sensitivity to tissue-specific differentiation. (uni-luebeck.de)

These findings highlight the importance of the SMC1-orchestrated intrachromosomal loop as a critical epigenetic barrier to the induction of pluripotency. (bath.ac.uk)
Complex haploinsufficiency in pluripotent cells yields somatic cells with DNA methylation abnormalities and pluripotency induction defects. (cdc.gov)

The links of in vivo reprogramming to pluripotency with tumorigenesis and teratoma formation, and the cross-talk with cellular senesce and tissue injury are also discussed. (springer.com)
Human cellular reprogramming to induced pluripotency is still an inefficient process, which has hindered studying the role of critical intermediate stages. (tigem.it)
Here we show that depleting Mbd3, a core member of the Mbd3/NuRD (nucleosome remodelling and deacetylation) repressor complex, together with OSKM transduction and reprogramming in naive pluripotency promoting conditions, result in deterministic and synchronized iPS cell reprogramming (near 100% efficiency within seven days from mouse and human cells). (umn.edu)
Subsequently, the latter interactions, which are largely depleted during early pre-implantation development in vivo, lead to a stochastic and protracted reprogramming trajectory towards pluripotency in vitro. (umn.edu)
The deterministic reprogramming approach devised here offers a novel platform for the dissection of molecular dynamics leading to establishing pluripotency at unprecedented flexibility and resolution. (umn.edu)
Taken together, we have identified a new regulatory role of Sirt6 in reprogramming and maintenance of pluripotency. (biomedcentral.com)
We hypothesized that the reprogramming of somatic cells towards pluripotency could be achieved in vivo by gene transfer of reprogramming factors. (nyu.edu)
It can be concluded that reprogramming somatic cells in vivo may offer a potential approach to induce enhanced pluripotency rapidly, efficiently, and safely compared to in vitro performed protocols and can be applied to different tissue types in the future. (nyu.edu)
Since HDAC inhibition facilitates cellular iPSC reprogramming, histone acetylation may play an important role in pluripotency. (biomedcentral.com)
Rationale: Skeletal myoblasts (SMs) with inherent myogenic properties are better candidates for reprogramming to pluripotency. (elsevierpure.com)

iPS cells showed many characteristics of human embryonic stem cells (hESCs) such as expression of pluripotency markers, reactivation of telomerase and the ability to form teratomas, demonstrating a potential to redifferentiate into descendants of all three embryonic lineages. (biologists.com)
Here, we have further characterized the process of PCMO generation with respect to markers of monocyte-to-macrophage transition and pluripotency. (uni-luebeck.de)
SiPS were positive for alkaline phosphatase, expressed SSEA1, and displayed a panel of embryonic stem (ES) cell-specific pluripotency markers. (elsevierpure.com)

Diagnostic clinical relevance of developmental pluripotency-associated 2 (DPPA2) in colorectal cancer. (nih.gov)
Chromatin modifier developmental pluripotency associated factor 4 (DPPA4) is a candidate gene for alcohol-induced developmental disorders. (bvsalud.org)

Enhances survival & cloning efficiency of ESC without affecting their pluripotency. (sigmaaldrich.com)
Prevents apoptosis and enhances the survival and cloning efficiency of dissociated hES cells without affecting their pluripotency. (sigmaaldrich.com)

Generation of induced pluripotent stem cells (iPSCs) by defined factors is an extremely inefficient process, because there is a strong epigenetic block preventing cells from achieving pluripotency. (bath.ac.uk)

Studies showed that any given population of ESCs could be very heterogeneous and that their potential pluripotency, or capacity to differentiate into other cell types, could vary both among cells from the same embryo and from one lineage to another. (eurekalert.org)
According to Panepucci, the human ESCs used in scientific research are usually in the intermediate development stage of primed pluripotency, when they have not yet differentiated but are primed, i.e., a step closer to assuming a distinct cell identity. (eurekalert.org)

Based on our analysis of this material, we established multiparameter phenotype profiles to determine the pluripotency stage of the cells and the effects of the miRNAs. (eurekalert.org)
If E-cadherin is absent, the stem cells lose their pluripotency. (sciencedaily.com)
In a first step they showed that the pluripotency of these stem cells is directly associated with the cell-adhesion molecule E-cadherin. (sciencedaily.com)
A collaboration involving the Babraham Institute, Cambridge Stem Cell Institute and the European Bioinformatics Institute, led by Dr Gabriella Ficz, Professor Wolf Reik and their colleagues, published results demonstrating that human stem cells can be reverted back to a base state, losing characteristics that mark them as a specific cell lineage and regaining the unrestricted potential (pluripotency) to develop into any cell type. (epigenie.com)
Find out more about stem cells and pluripotency in Cell, September 2014 . (epigenie.com)
you(r cells) are never too old for pluripotency! (biologists.com)
The authors then repeated this procedure with cells from donors 92, 94, 96 and 101 years of age and again were successful in generating iPS cells with the same efficiency, making these the oldest human donors so far whose cells were reprogrammed for pluripotency. (biologists.com)
The Pluripotency Check PCR Kit contains all the necessary reagents to confirm that your mouse embryonic stem (ES) or induced pluripotent (iPS) cells are indeed pluripotent. (takarabio.com)
The kit includes everything for start-to-finish confirmation of your cells' pluripotency status, using any combination of primer sets, or the entire panel. (takarabio.com)
The Pluripotency Check PCR Kit can be used to validate the pluripotent status (ability to differentiate into specialized cells of all three germ layers) of your stem cell culture. (takarabio.com)
A process where fully differentiated or specialized cells revert to pluripotency or a less differentiated cell type. (bvsalud.org)

What is cellular totipotency how does it differ from pluripotency? (careersoudan29.com)

Interferon gamma represents an example of pluripotency in itself. (wikipedia.org)

This pluripotency depends on a molecule -- E-cadherin -- hitherto primarily known for its role in mediating cell-cell adhesion. (sciencedaily.com)

Their capacity to originate any type of tissue is greater at that stage," said Rodrigo Alexandre Panepucci ( https://bv.fapesp.br/en/pesquisador/57383/rodrigo-alexandre-panepucci/ ), a researcher at the Ribeirão Preto Blood Center and principal investigator of the study. (eurekalert.org)

Objective: To reprogram SMs to pluripotency and show that reprogrammed SMs (SiPS) express embryonic gene and microRNA profiles and that transplantation of predifferentiated cardiac progenitors reduce tumor formation. (elsevierpure.com)

Après des études de médecine à l'Université de Genève et une formation clinique en pathologie, médecine interne et maladies infectieuses à Genève et au Massachusetts General Hospital de Boston, Didier Trono s'engage dans une carrière scientifique au Whitehead Institute du MIT. (epfl.ch)

When genes known to control pluripotency were deleted-genes such as OCT4 and SOX2-the hESCs surprisingly increased their resistance to death, indicating that under normal circumstances pluripotency regulators also contribute to apoptosis pathways. (genengnews.com)

Molecular aspects of epigenetic reprogramming during the acquisition of pluripotency, as well as tumorigenic potential, have also been discussed, thus providing an understanding of the factors that can improve the former without increasing the possibility of neoplastic transformation. (tubitak.gov.tr)
Furthermore, established C-iPSC lines were shown to be capable of forming teratomas in vivo, containing cell types and tissues from each of the embryonic germ layers, fully consistent with their acquisition of pluripotency. (ox.ac.uk)
Acquisition of pluripotency by transcriptional regulatory factors is an initial developmental event that is required for regulation of cell fate and lineage specification during early embryonic development. (biomedcentral.com)
There are also species-specific maternally inherited transcriptional factors and their intricate transcriptional networks important in the acquisition of pluripotency. (biomedcentral.com)
In avian species, however, the core transcriptional network that governs the acquisition of pluripotency during early embryonic development is not well understood. (biomedcentral.com)
These results suggest that chickens have a unique pluripotent circuitry since maternally inherited c NANOG and cSOX3 may play an important role in the initial acquisition of pluripotency. (biomedcentral.com)
Moreover, the acquisition of pluripotency in chicken embryos occurs at around EGK.VI to VIII. (biomedcentral.com)
The acquisition of pluripotency plays a pivotal role in determining developmental fate during early vertebrate embryogenesis. (biomedcentral.com)
However, the acquisition of pluripotency and the core pluripotency circuitry during early embryonic development has yet to be investigated in detail in birds. (biomedcentral.com)
However, the intricate changes of transcriptional network under regulation of core pluripotency circuitry during the acquisition of pluripotency in avian species are not clear. (biomedcentral.com)

This cell state transition is recapitulated by mouse embryonic stem cells (ESCs), which undergo pluripotency progression in defined conditions in vitro. (uni-koeln.de)
10. Li X, Jia Q, Di K, Gao S, Wen X, Zhou R, Wei W, Wang LZ (2007) Passage number affects the pluripotency of mouse embryonic stem cells as judged by tetraploid embryo aggregation. (prelekara.sk)

This position is focused on exploring genetic and epigenetic determinants of reprogramming and induced pluripotency. (nih.gov)
After observing significant disease-modifying effects, the CM was characterized biochemically by fractionation, comparative proteomics, and epigenetic screens for the dependence on pluripotency. (springer.com)
Induced pluripotency of a differentiated cell is mediated by sequential cascades of genetic and epigenetic reprogramming of somatic histone and DNA CpG methylation marks. (johnshopkins.edu)
Major conclusions: Through a variety of mechanisms, ncRNAs regulate constellations of key pluripotency genes and epigenetic regulators, and thus critically determine induction and maintenance of the pluripotent state. (johnshopkins.edu)

StemCells, Inc. (Nasdaq:STEM) announced today that newly published independent research demonstrates that its GS2-M™ cell culture media formulation enhances the pluripotency of human embryonic stem (ES) and induced pluripotent stem (iPS) cells. (news-medical.net)
Huo, JS & Zambidis, ET 2013, ' Pivots of pluripotency: The roles of non-coding RNA in regulating embryonic and induced pluripotent stem cells ', Biochimica et Biophysica Acta - General Subjects , vol. 1830, no. 2, pp. 2385-2394. (johnshopkins.edu)

The purpose of this initiative is to advance the fundamental understanding of the basis of pluripotency and the molecular events and mechanisms of reprogramming, including the epigenetics and epigenomics of the pluripotent and reprogrammed states. (nih.gov)
Here, we discuss the reconstruction of regulatory networks in individual cells using novel single cell transcriptomics and proteomics, in order to expand our understanding of the molecular basis of pluripotency, including the role of cell-cell variability within ES cell populations, and ways in which networks may be controlled in order to reliably manipulate cell behaviour. (biorxiv.org)
Deciphering the molecular basis of pluripotency is fundamental to our understanding of development and embryonic stem (ES) cell function. (berkeley.edu)

We are interested in applying these methods to fish, and here, we examined whether mRNA expression of germline-specific genes (vasa, nanos2, -3) and pluripotency factors (oct4, sox2, c-myc, nanog) is inducible in somatic cells of Japanese medaka (Oryzias latipes). (elsevierpure.com)
Genome-wide ChIP-seq mapping showed that Brd4 and H4 acetylation are co-occupied in the genome, upstream of core pluripotency genes such as Oct4 and Nanog in ESCs and lineage-specific genes in embryoid bodies (EBs). (elsevierpure.com)
Using this method, we demonstrate that the pluripotency TF NANOG is significantly more dependent on the SWI/SNF family ATPase BRG1 for association with its genomic targets in vivo than in cultured cells, a finding that could not have been made using traditional approaches. (umassmed.edu)
The results showed that exogenous IL-6 promoted mESCs proliferation and favored maintenances of pluripotency, evidenced by up-regulated mRNA expression of oncogenes (c-fos, c-jun) and stemness markers (oct4, nanog), down-regulated mRNA expression of germ layer genes (branchyury, FLK-1, pecam, ncam , sox17), and increased phosphorylation of ERK1/2 and STAT3. (bvsalud.org)
Retinol activates the endogenous machinery to cause over expression of pluripotency specific genes such as Nanog. (nih.gov)

We study molecular mechanisms behind T cell reconstitution in rheumatoid arthritis (RA) by asking if PBX1, a transcription factor that maintains stem cell pluripotency, predicts antirheumatic treatment response. (acrabstracts.org)

We discovered that the chromatin-modifying complex SAGA and in particular its subunit TADA2B are central regulators of pluripotency, survival, growth, and lineage specification," the article's authors wrote. (genengnews.com)
Our goal in Aim 3 is to discover new stem cell regulators, both factors that positively and negatively regulate self-renewal and pluripotency. (ca.gov)
Moving toward the specific goal of finding positive regulators of pluripotency, we found that the key tool we need, something called an hESC cDNA retroviral library, does not commercially exist. (ca.gov)
Our proteomics-based studies in the first year of the award have yielded some very interesting preliminary candidates in ESC, including some known, important pluripotency regulators. (ca.gov)
Transcriptome profiles for 73 ESC lines deficient for regulators of the exit from naive pluripotency predominantly manifest delays on the trajectory from naive to formative epiblast. (uni-koeln.de)
Here, we report that depleting Spt6 in embryonic stem cells (ESCs) reduced expression of pluripotency factors, increased expression of cell-lineage-affiliated developmental regulators, and induced cell morphological and biochemical changes indicative of ESC differentiation. (nih.gov)

Primordial germ cells (PGCs) provide an excellent tool to better understand ancestor?descendent relationships as well as the efficiency and molecular mechanisms governing pluripotency in the reprogramming of somatic cells, since the latter type of cells have a relatively lower efficiency of conversion to pluripotent cells. (tubitak.gov.tr)

Expert opinion: Here, we review our current understanding on the mechanisms that dictate the spectrum of pluripotency levels. (medscape.com)
In the course of these studies we have identified specific molecules and pathways downstream of Myc that represent candidate mechanisms by which it orchestrates pluripotency and self-renewal. (ca.gov)
We are also investigating the signaling mechanisms of Vitamin A/retinol in pluripotency and self-renewal of ES and iPS cells. (nih.gov)

Our data show that mRNA expression of genes relevant to germ cell specification and pluripotency can be induced in fish somatic cells by exposure to DEN, suggesting the possibility of efficient and rapid cell reprogramming of fish somatic cells. (elsevierpure.com)

These data suggest a model in which SMARCAD1 regulates naive pluripotency by interacting with H3R26Cit and suppressing heterochromatin formation. (pucv.cl)

Increased Smarcad1 expression correlated with naive pluripotency in pre-implantation embryos. (pucv.cl)

At first, we constructed a pluripotency assay system that the candidate factors are introduced into neonate fibroblasts via retrovirus vectors. (nih.gov)
Exploring induced pluripotency in human fibroblasts via construction, validation, and application of a gene regulatory network. (nih.gov)

INTRODUCTION: Pluripotency emerges transiently during embryogenesis in two main forms with different developmental potential, termed naïve and primed states. (medscape.com)

In both hESC and mESC we have found that Myc genes are essential for normal pluripotency and self-renewal. (ca.gov)

Expression of pluripotency-related genes in human glioblastoma. (physiciansweekly.com)

Our findings uncover a dichotomous molecular function for the reprogramming factors, serving to reactivate endogenous pluripotency networks while simultaneously directly recruiting the Mbd3/NuRD repressor complex that potently restrains the reactivation of OSKM downstream target genes. (umn.edu)
Others had suggested that neural crest cells have the unique ability to reactivate their pluripotency after gastrulation. (nih.gov)

In the mammalian embryo, epiblast cells must exit the naive state and acquire formative pluripotency. (uni-koeln.de)

Selective downregulation of pluripotency factors upon Spt6 depletion may be mechanistically explained by its enrichment at ESC super-enhancers, where Spt6 controls histone H3K27 acetylation and methylation and super-enhancer RNA transcription. (nih.gov)
Of these, we show that the pioneering activity of the maternal pluripotency factors Pou5f3 and Sox3 determines competence for germ layer formation by extensively remodelling compacted chromatin before the onset of inductive signalling. (crick.ac.uk)
The evolutionarily conserved core transcriptional factors regulating the pluripotency network in fishes, amphibians, and mammals have been elucidated. (biomedcentral.com)
Although maternally inherited core transcription factors for the initial acquisition and organization of pluripotency are unique to each vertebrate species, their network in vertebrates is well conserved. (biomedcentral.com)

As the result, the set of Oct3/4, Sox2, c-Myc, and Klf-4 gave rise to drug resistant colonies implying potential pluripotency. (nih.gov)

In the course of this work, the researchers introduced genetic alterations that caused pluripotency dissolution and simultaneously increased apoptosis resistance. (genengnews.com)

Our results indicate that Chd1 is essential for open chromatin and pluripotency of embryonic stem cells, and for somatic cell reprogramming to the pluripotent state. (mssm.edu)

A collaboration involving the Babraham Institute, Cambridge Stem Cell Institute and the European Bioinformatics Institute, led by Dr Gabriella Ficz, Professor Wolf Reik and their colleagues, published results demonstrating that human stem cells can be reverted back to a base state, losing characteristics that mark them as a specific cell lineage and regaining the unrestricted potential (pluripotency) to develop into any cell type. (epigenie.com)
Our findings suggest that exit from ground state of pluripotency is unaffected by mechanical signals, but that these signals could become important during the next stage of lineage specification. (cam.ac.uk)

However, our understanding of the molecular cascades and gene networks involved in the exit from naive pluripotency remains fragmentary. (uni-koeln.de)

Meanwhile, Oct25 ( Pou5f3.2 ), Oct60 ( Pou5f3.3 ), and Sox3 are maternally inherited to establish the pluripotency network and initiate the maternal to zygotic transition in Xenopus. (biomedcentral.com)

Subsequently, the latter interactions, which are largely depleted during early pre-implantation development in vivo, lead to a stochastic and protracted reprogramming trajectory towards pluripotency in vitro. (umn.edu)

This stem cell superpower is called pluripotency. (nih.gov)

General significance: A further understanding of the roles of ncRNAs in regulating pluripotency may help assess the quality of human iPSC reprogramming. (johnshopkins.edu)

In addition, we found that the pluripotency-related genes such as cENS-1 , cKIT , cLIN28A , cMYC , cPRDM14 , and cSALL4 began to be dramatically upregulated between EGK.VI and VIII. (biomedcentral.com)

Anatomy48

Organisms15

Diseases5

Chemicals and Drugs87

Analytical, Diagnostic and Therapeutic Techniques and Equipment36

Phenomena and Processes66

Disciplines and Occupations5

Information Science4

OCT49

Genes6

Sox22

Loss of pluripotency1

Transcriptional1

Maintain pluripotency1

Differentiation5

Induction2

Reprogramming10

Markers3

Developmental2

Enhances2

Pluripotent stem1

ESCs2

Cells11

Cellular1

Represents1

Role1

Https1

Show1

Formation1

Deleted-genes1

Acquisition of pluripotency10

Mouse embryonic stem2

Epigenetic4

Induced plur2

Basis of pluripotency3

Nanog5

Stem cell pluripotency1

Regulators6

Reprogramming of somatic cells1

Mechanisms3

Somatic cells1

Regulates1

Embryos1

Fibroblasts2

Embryogenesis1

Self-renewal1

Expression1

Reactivate2

Naive state1

Factors4

Sox21

Apoptosis1

Chromatin1

Lineage2

Understanding of the molecular1

Initiate1

Vitro1

Cell1

Human1

Found1