• Objective】 Through genome sequencing and bioinformatics analysis of GD2107 strain of multi-drug resistant Actinobacillus pleuropneumoniae (APP),the genome database information of APP was enriched,the phylogenetic tree based on Apx Ⅳ gene was constructed,and the evolutionary relationship of the strain was analyzed,so as to provide reference for exploring the pathogenic mechanism of APP and clinical prevention and control of porcine infectious porcine contagious pleuropneumia (PCP). (chvm.net)
  • Whole Genome Sequencing and Biological Characteristics Analysis of Multidrug-resistant Actinobacillus pleuropneumoniae GD2107 Strain[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 4019-4031. (chvm.net)
  • 1] ZHANG L,WU X,HUANG Z,et al.Pharmacokinetic/pharmacodynamic assessment of cefquinome against Actinobacillus pleuropneumoniae in a piglet tissue cage infection model[J]. Veterinary Microbiology ,2018,219(6):100-106. (chvm.net)
  • 3] SHIN M,KANG M L,CHA S B,et al.An immunosorbent assay based on the recombinant ApxⅠa,ApxⅡa,and ApxⅢa toxins of Actinobacillus pleuropneumoniae and its application to field sera[J]. Journal of Veterinary Diagnostic Investigation ,2011,23(4):736-742. (chvm.net)
  • 5] STRINGER O W,LI Y,BOSS J T,et al.Rapid detection of Actinobacillus pleuropneumoniae from clinical samples using recombinase polymerase amplification[J]. Frontiers in Veterinary Science ,2022,9:805382. (chvm.net)
  • 18. Ito H, Takahashi S, Asai T, Tamura Y, Yamamoto K. Isolation and molecular characterization of a urease-negative Actinobacillus pleuropneumoniae mutant. (gifu-u.ac.jp)
  • Treatment of acute outbreaks of swine respiratory disease caused by strains of Actinobacillus pleuropneumoniae and Pasteurella multocida susceptible to florfenicol. (farmacy.co.uk)
  • Treatment of respiratory tract infections due to strains of Mannheimia haemolytica, Pasteurella multocida and Histophilus somni susceptible to florfenicol. (farmacy.co.uk)
  • Conclusion】 In this study, we completed the whole genome sequencing and bioinformatics analysis of the multi-drug resistant strain GD2107, fully understood the structure and function of the genome of the strain, and explored the related genes in drug resistance and pathogenic mechanism. (chvm.net)
  • We propose that human-like H3N2 influenza A strains may remain invariant for long periods in swine, which may serve as a reservoir for human pandemics. (microbiologyresearch.org)
  • Prevalence of antibodies to swine influenza virus, porcine adenovirus type 4 and Haemophilus pleuropneumoniae in Quebec pig farms with respiratory problems. (microbiologyresearch.org)
  • Both strains were grown in minimal medium without lysine and thymidine, indicating that deletion of the thyX gene had no effect on the expression of genes downstream of thyX or on lysine biosynthesis, and that thyX is not an essential gene in C. glutamicum. (alk-inhibitors.com)
  • Their success in clearing or inactivating a pathogen will depend on the amount of antibodies produced and on the extent to which those antibodies are effective at countering the strain of the pathogen involved, since different strains may be differently susceptible to a given immune reaction. (webot.org)
  • 6. Ichikawa T, Oshima M, Yamagishi J, Muramatsu C, Asai T. Changes in antimicrobial resistance phenotypes and genotypes in Streptococcus suis strains isolated from pigs in the Tokai area of Japan. (gifu-u.ac.jp)
  • Method】 The drug resistance spectrum of the isolated strains was determined by drug sensitivity test. (chvm.net)
  • 2002). All wild type, mutant KH1 and thyX complemented KH2 strains were grown in MCGC minimal medium containing isocitrate and glucose with 3 μM WR99210. (alk-inhibitors.com)
  • The thyX deletion mutant, KH1, lost viability much more rapidly in the stationary growth phase than either the parental wild type or the ThyX complemented KH3 strains. (alk-inhibitors.com)
  • We demonstrated that a thyX knockout mutant strain was more sensitive to a DHFR inhibitor, WR99210, compared with a wild-type strain. (alk-inhibitors.com)
  • Of the four pandemic strains of human influenza A virus observed this century, the 1977 virus strain was very similar in all genes to a 1950 isolate. (microbiologyresearch.org)
  • Because of deletion mutation in the thyX region, it produced a 350-bp fragment (Fig. 1c, lane 7), whereas wild-type strain produced an 1190-bp fragment (Fig. 1c, lane 4). (alk-inhibitors.com)
  • The KH1 strain appeared to be more sensitive than wild-type strain to WR99210. (alk-inhibitors.com)
  • As results of laboratory experiments we received the following half-lethal doses (LD50) of cypermethrin ("Samarovka-insecticide") 0.057 (0.036 ÷ 0.092) and 0,091 (0.048 ÷ 0.171) μg of active agent per insect for flies of the laboratory strain and the wild population, respectively. (vetvrach-vnivi.ru)
  • LD50 of deltamethrin ("FAS") was significantly less 0.0045 (0.0025 ÷ 0.0079) and 0.0130 (0.0082 ÷ 0.0207) μg of active agent per insect for flies of the laboratory strain and the wild population, respectively. (vetvrach-vnivi.ru)
  • Deltamethrin as a part of "FAS"product was more active than cypermethrin in "Samarovka-insecticide" product against mature M.domestica of the laboratory strain and the wild population. (vetvrach-vnivi.ru)
  • This disease is airborne, and the wild strains can easily break through the defense of a sound vaccine prevention and control system, resulting in rapid proliferation. (biomedcentral.com)
  • Therefore, the major contribution to the higher protein-to-lipid ratio in the A. oryzae strains comes from the significant increase of the area of both Amide I and Amide II. (pdgfrsignals.com)
  • For the biofilm assay, strain 719, a field isolate of A. pleuropneumoniae serovar 1, was mixed with swine isolates of Streptococcus suis , Bordetella bronchiseptica , Pasteurella multocida , Staphylococcus aureus or Escherichia coli , and deposited in 96-well microtiter plates. (biomedcentral.com)
  • The existence of nonencapsulated A. pleuropneumoniae serovar K12:O3 would hamper precise serodetection. (bvsalud.org)
  • To date, 15 serotypes of A . pleuropneumoniae have been described based on capsular antigens [ 3 , 10 ]. (vetres.org)
  • It has also been reported that only 2 of the 15 A . pleuropneumoniae reference strains, representing serotypes 5b and 11, were able to form a biofilm in vitro and that the transition from a biofilm-positive to biofilm-negative phenotype was irreversible [ 21 ]. (vetres.org)
  • Several respiratory tract infections are associated with biofilm formation, and A. pleuropneumoniae has the ability to form biofilms in vitro. (biomedcentral.com)
  • In this study, we compared biofilm formation by the serotype 1 reference strain S4074 of A . pleuropneumoniae grown in five different culture media. (vetres.org)
  • We observed that strain S4074 of A . pleuropneumoniae is able to form biofilms after growth in one of the culture conditions tested brain heart infusion (BHI medium, supplier B). Confocal laser scanning microscopy using a fluorescent probe specific to the poly-N-acetylglucosamine (PGA) polysaccharide further confirmed biofilm formation. (vetres.org)
  • Transcriptional profiles of A . pleuropneumoniae S4074 following growth in BHI-B, which allowed a robust biofilm formation, and in BHI-A, in which only a slight biofilm formation was observed, were compared. (vetres.org)
  • Our data indicate that A . pleuropneumoniae has the ability to form biofilms under appropriate growth conditions and transition from a biofilm-positive to a biofilm-negative phenotype was reversible. (vetres.org)
  • However, genes involved in the biosynthesis of O-polysaccharide of the 3 isolates were highly similar to those of reference strains of serovars 3, 6, 8, 15, 17, and 19. (bvsalud.org)
  • In A . pleuropneumoniae , the formation of biofilms on polystyrene microtiter plate is dependent on the production of poly-N-acetylglucosamine (PGA) a linear polymer of N-acetylglucosamine residues in β(1,6) linkage [ 17 , 20 ]. (vetres.org)
  • The evolutionary relationship showed that the strain had a certain regional epidemic, which provided a reference for preventing the epidemic of PCP and exploring the pathogenic mechanism of APP. (chvm.net)
  • Based on the CFU results, A. pleuropneumoniae was able to grow with every species tested in the absence of pyridine compounds in the culture media. (biomedcentral.com)
  • Phylogenetic tree analysis showed that the evolutionary relationship between this strain and APP strain from China (CP063424.1) was the closest. (chvm.net)
  • Therefore, it is extremely important to study the genetic characteristics of the most prevalent strains causing bacteremia to more effectively target the strategies for controlling these infections. (mdpi.com)