• Objective】 Through genome sequencing and bioinformatics analysis of GD2107 strain of multi-drug resistant Actinobacillus pleuropneumoniae (APP),the genome database information of APP was enriched,the phylogenetic tree based on Apx Ⅳ gene was constructed,and the evolutionary relationship of the strain was analyzed,so as to provide reference for exploring the pathogenic mechanism of APP and clinical prevention and control of porcine infectious porcine contagious pleuropneumia (PCP). (chvm.net)
  • Whole Genome Sequencing and Biological Characteristics Analysis of Multidrug-resistant Actinobacillus pleuropneumoniae GD2107 Strain[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 4019-4031. (chvm.net)
  • Actinobacillus pleuropneumoniae (previously Haemophilus pleuropneumoniae), is a Gram-negative, facultative anaerobic, respiratory pathogen found in pigs. (wikipedia.org)
  • The typical presentation of A. pleuropneumoniae in pigs is the characteristic demarcated lesions in the middle, cranial, and caudal lobes of the lungs. (wikipedia.org)
  • Actinobacillus pleuropneumoniae hfq mutants strains are attenuated for virulence in pigs, impaired in the ability to form biofilms, and more susceptible to stress, but knowledge of the extent of sRNA involvement is limited. (bvsalud.org)
  • Salmonella enterica serovar Typhimurium again predominated with unremarkable antimicrobial susceptibility patterns. (thepigsite.com)
  • Nineteen different serotype variants (serovars) have been recognized for A. pleuropneumoniae, based on the different capsular polysaccharides exhibited. (wikipedia.org)
  • Here, using A. pleuropneumoniae strain MIDG2331 (serovar 8), 14 sRNAs were identified by co-immunoprecipitation with Hfq and the expression of eight, identified as trans-acting sRNAs, were confirmed by Northern blotting. (bvsalud.org)
  • Actinobacillus pleuropneumoniae was found to be the causative agent for up to 20% of all bacterial pneumonia cases in swine. (wikipedia.org)
  • Actinobacillus pleuropneumoniae is a nonmotile, Gram-negative, encapsulated coccobacillus bacterium found in the family Pasteurellaceae. (wikipedia.org)
  • 5] STRINGER O W,LI Y,BOSS J T,et al.Rapid detection of Actinobacillus pleuropneumoniae from clinical samples using recombinase polymerase amplification[J]. Frontiers in Veterinary Science ,2022,9:805382. (chvm.net)
  • Differences in virulence potential, immunogenicity, and worldwide geographical distribution contribute to the diversity of the A. pleuropneumoniae serotypes. (wikipedia.org)
  • Several virulence factors account for the remarkable pathogenicity of A. pleuropneumoniae. (wikipedia.org)
  • This work increases our understanding of the multilayered and complex nature of the influence of Hfq-dependent sRNAs on the physiology and virulence of A. pleuropneumoniae. (bvsalud.org)
  • 3] SHIN M,KANG M L,CHA S B,et al.An immunosorbent assay based on the recombinant ApxⅠa,ApxⅡa,and ApxⅢa toxins of Actinobacillus pleuropneumoniae and its application to field sera[J]. Journal of Veterinary Diagnostic Investigation ,2011,23(4):736-742. (chvm.net)
  • Prepared from formol-killed cultures of Leptospira interrogans serovar hardjo grown in a synthetic medium free from all proteins, with potash alum as adjuvant. (msd-animal-health.ie)
  • 1] ZHANG L,WU X,HUANG Z,et al.Pharmacokinetic/pharmacodynamic assessment of cefquinome against Actinobacillus pleuropneumoniae in a piglet tissue cage infection model[J]. Veterinary Microbiology ,2018,219(6):100-106. (chvm.net)
  • Actinobacillus pleuropneumoniae (previously Haemophilus pleuropneumoniae), is a Gram-negative, facultative anaerobic, respiratory pathogen found in pigs. (wikipedia.org)
  • As a facultative anaerobic pathogen, A. pleuropneumoniae may need CO2 to grow. (wikipedia.org)
  • Based on 12 genomes of corresponding serovar reference strains including three publicly available complete genomes (serovars 3, 5b, and 7) of this bacterium, we performed a comprehensive analysis of comparative genomics and first reported a global genomic characterization for this pathogen. (nih.gov)
  • It has been reported in the literature that only two of the reference strains of the swine pathogen Actinobacillus pleuropneumoniae , representing serotypes 5b and 11, were able to form biofilm in vitro. (vetres.org)
  • Actinobacillus pleuropneumoniae , a member of the Pasteurellaceae , is an important swine pathogen responsible for economic losses in the swine industry. (vetres.org)
  • C. trachomatis is primarily a pathogen of humans and has been divided into 15 serovars. (docksci.com)
  • C. pneumoniae, also a pathogen of humans, has one known serovar. (docksci.com)
  • In order to better interpret the genetic background of serotypic diversity, nine genomes of A. pleuropneumoniae reference strains of serovars 1, 2, 4, 6, 9, 10, 11, 12, and 13 were sequenced by using rapid high-throughput approach. (nih.gov)
  • It has also been reported that only 2 of the 15 A . pleuropneumoniae reference strains, representing serotypes 5b and 11, were able to form a biofilm in vitro and that the transition from a biofilm-positive to biofilm-negative phenotype was irreversible [ 21 ]. (vetres.org)
  • However, genes involved in the biosynthesis of O-polysaccharide of the 3 isolates were highly similar to those of reference strains of serovars 3, 6, 8, 15, 17, and 19. (bvsalud.org)
  • these five strains are presumed to represent at least two additional serovars. (docksci.com)
  • Using serovarspecific MAbs, I have identified four serovars within the avian strains (2). (docksci.com)
  • Nineteen different serotype variants (serovars) have been recognized for A. pleuropneumoniae, based on the different capsular polysaccharides exhibited. (wikipedia.org)
  • To date, 15 serotypes of A . pleuropneumoniae have been described based on capsular antigens [ 3 , 10 ]. (vetres.org)
  • Three Actinobacillus pleuropneumoniae isolates from clinical cases of porcine pleuropneumonia were positive by capsular serovar 12-specific PCR assay, but not reactive to antiserum prepared against serovar 12 using the rapid slide agglutination (RSA) test. (bvsalud.org)
  • Nucleotide sequence analysis revealed that genes involved in the biosynthesis of capsular polysaccharide of the 3 isolates were identical or nearly identical to those of serovar 12. (bvsalud.org)
  • Serotyping of Chlamydia psittaci isolates using serovar-specific monoclonal antibodies with the microimmunofluorescence test. (docksci.com)
  • The use of the indirect microimmunofluorescence (IMIF) test with serovar-specific MAbs meets these requirements and has proven to be usable with C. trachomatis isolates (22, 43). (docksci.com)
  • Earlier, I and others reported on the production of serovar-specific MAbs to C. psittaci and demonstrated that they could be used for serotyping isolates in the indirect fluorescent antibody technique (1-3). (docksci.com)
  • Transcriptional profiles of A . pleuropneumoniae S4074 following growth in BHI-B, which allowed a robust biofilm formation, and in BHI-A, in which only a slight biofilm formation was observed, were compared. (vetres.org)
  • Our data indicate that A . pleuropneumoniae has the ability to form biofilms under appropriate growth conditions and transition from a biofilm-positive to a biofilm-negative phenotype was reversible. (vetres.org)
  • PGA is a substrate for dispersin B (DspB), a biofilm-releasing glycosyl hydrolase produced by Aggregatibacter ( Actinobacillus ) actinomycetemcomitans and A . pleuropneumoniae [ 20 , 22 ]. (vetres.org)
  • Several respiratory tract infections are associated with biofilm formation, and A. pleuropneumoniae has the ability to form biofilms in vitro. (biomedcentral.com)
  • In the presence of E. coli , A. pleuropneumoniae only formed a weak biofilm. (biomedcentral.com)
  • species= Actinobacillus pleuropneumoniae serovar 7 str. (lbl.gov)
  • Based on the CFU results, A. pleuropneumoniae was able to grow with every species tested in the absence of pyridine compounds in the culture media. (biomedcentral.com)
  • In conclusion, A. pleuropneumoniae apparently is able to satisfy the requirement of pyridine compounds through of other swine pathogens by cross-feeding, which enables A. pleuropneumoniae to grow and form multi-species biofilms. (biomedcentral.com)
  • The Gram-negative bacterium Actinobacillus pleuropneumoniae is the etiologic agent of porcine contagious pleuropneumoniae, a lethal respiratory infectious disease causing great economic losses in the swine industry worldwide. (nih.gov)
  • Actinobacillus pleuropneumoniae is the etiologic agent of porcine contagious pleuropneumonia, which causes important worldwide economic losses in the swine industry. (biomedcentral.com)
  • 5] STRINGER O W,LI Y,BOSS J T,et al.Rapid detection of Actinobacillus pleuropneumoniae from clinical samples using recombinase polymerase amplification[J]. Frontiers in Veterinary Science ,2022,9:805382. (chvm.net)
  • It would provide needed information about whether a given serovar is limited to specific clinical syndromes and/or to specific anatomical sites in the host. (docksci.com)
  • Differences in virulence potential, immunogenicity, and worldwide geographical distribution contribute to the diversity of the A. pleuropneumoniae serotypes. (wikipedia.org)
  • In A . pleuropneumoniae , the formation of biofilms on polystyrene microtiter plate is dependent on the production of poly-N-acetylglucosamine (PGA) a linear polymer of N-acetylglucosamine residues in β(1,6) linkage [ 17 , 20 ]. (vetres.org)
  • Actinobacillus pleuropneumoniae was found to be the causative agent for up to 20% of all bacterial pneumonia cases in swine. (wikipedia.org)
  • For a serotyping system to be successful for C. psittaci, it must be easy to perform, be reproducible, require a minimum of sample and materials, and be expandable to include new serovars. (docksci.com)
  • It would provide a tool for epidemiological studies to determine the distribution of a given serovar in both a geographic area and a host range. (docksci.com)