Our objective in this study was to determine the extent to which tocotrienols inhibit platelet aggregation and reduce coronary thrombosis, a major risk factor for stroke in humans. (biomedcentral.com)
Metabolites2
In general, various phospholipase A2s and diacylglycerol lipases are activated during cell stimulation, proceed to release these fatty acids from their storage sites, and thereby are key regulators in the formation of lipoxygenase-dependent metabolites. (wikipedia.org)
Furthermore, di-GA inhibited the generation of lymphendothelial gaps by cancer cell spheroid-secreted lipoxygenase metabolites. (nature.com)
ALOX52
The 6 human lipoxygenases along with some of the major products that they make, as well as some of their associations with genetic diseases, are as follows: Arachidonate 5-lipoxygenase (ALOX5) (EC 1.13.11.34InterPro: IPR001885), also termed 5-lipoxygenase, 5-LOX, and 5-LO. (wikipedia.org)
ALOX5 arachidonate 5-lipoxygenase: macrophages infected with Candida albicans, Aspergillus flavus or Aspergillus fumigatus or treated with Curdlan, a selective agonist of pattern recognition receptor for fungi Dectin-1, displays increased expression of ALOX5, ALOX15 and PTGS2 Karnam et al, 2015 . (polygenicpathways.co.uk)
including effects on platelet aggregation, vascular permeability, and inflammatory and immunological responses, would represent a novel means of regulating the microenvironment surrounding helminthic parasites. (grantome.com)
a mixture of α- + γ- + δ-tocotrienols) on in vivo platelet thrombosis and ex vivo platelet aggregation (PA) after intravenous injection in anesthetized dogs, by using a mechanically stenosed circumflex coronary artery model (Folts' cyclic flow model). (biomedcentral.com)
The present results indicate that intravenously administered tocotrienols inhibited acute platelet-mediated thrombus formation, and collagen and ADP-induced platelet aggregation. (biomedcentral.com)
The inhibitory effects of α-tocopherol on platelet function in vivo and ex vivo have been well established [ 14 - 18 ], but inhibition of platelet aggregation by α-tocopherol in vitro requires non-physiologically high levels of α-tocopherol [ 14 ]. (biomedcentral.com)
Pathways3
In addition, cells, when so activated, may transfer their released polyunsaturated fatty acids to adjacent or nearby cells which then metabolize them through their lipoxygenase pathways in a process termed transcellular metabolism or transcellular biosynthesis. (wikipedia.org)
Part III: Products of the Lipoxygenase Pathways and their Receptors. (edu.au)
They are part of a much larger group of compounds, the eicosanoids, which, although ubiquitous as a group, are produced by a variety of cell type-, tissue-, and species-specific biosynthetic pathways. (jci.org)
Changes in dietary fatty acids, specifically the polyunsaturated fatty acids of the ω-3 and ω-6 families and some derived eicosanoids from lipoxygenases, cyclooxygenases, and cytochrome P-450, seem to control the activity of transcription factor families involved in cancer cell proliferation or cell death. (springer.com)
The AA, a normal component of cell membrane phospholipids, serve as substrate for prostaglandin endoperoxide (PGH) syntases-1 and -2, also known as cycloxygenase (COX)-1 and -2, lipoxygenase (5-, 12-, or 15) (LO), or cytochrome p450 enzymes [ 18 ]. (hindawi.com)
ALOXE31
Certain types of the lipoxygenases, e.g. human and murine 15-lipoxygenase 1, 12-lipoxygenase B, and ALOXE3, are capable of metabolizing fatty acid substrates that are constituents of phospholipids, cholesterol esters, or complex lipids of the skin. (wikipedia.org)
Protein2
Based on detailed analyses of 15-lipoxygenase 1 and stabilized 5-lipoxygenase, lipoxygenase structures consist of a 15 kilodalton N-terminal beta barrel domain, a small (e.g. ~0.6 kilodalton) linker inter-domain (see Protein domain § Domains and protein flexibility), and a relatively large C-terminal catalytic domain which contains the non-heme iron critical for the enzymes' catalytic activity. (wikipedia.org)
In response to the presence of certain activating substances, including oxidized LDL, monocyte chemoattractant protein 1 (MCP-1), interleukin (IL)-8, and platelet-derived growth factor (PDGF), leukocytes migrate into the wall of the artery (see the image below). (medscape.com)
Diabetes mellitus1
Conversely, reactive oxygen species may favor the development of type 2 diabetes mellitus, mainly through the induction of insulin resistance. (biomedcentral.com)
Inhibitor1
Effect of 15-HETE on the 5-Lipoxygenase Pathway in Neutrophils: Genuine Inhibitor or Alternative Substrate? (edu.au)
Prostaglandins1
COX2 converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis, including production of inflammatory prostaglandins. (thermofisher.com)
Pathway1
Collectively, these landmark discoveries provided initial insight into the COX pathway of arachidonate metabolism. (medscape.com)
Derivatives2
The effects of filarial arachidonate derivatives on human eosinophil, neutrophil, lymphocyte and platelet functions will be assessed. (grantome.com)
Further, by characterizing the biological activities of any filarial arachidonate derivatives, these studies may provide insights into novel mechanisms of parasite pathogenicity and may suggest rational approaches to the development of antifilarial agents. (grantome.com)
Pathological1
Tocotrienols, given intravenously, could potentially prevent pathological platelet thrombus formation and thus provide a therapeutic benefit in conditions such as stroke and myocardial infarction. (biomedcentral.com)
Canine2
Description: A competitive ELISA for quantitative measurement of Canine Arachidonate 15 lipoxygenase B(ALOX15B) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (myelisakit.com)
Next, pharmacokinetic studies were carried out and tocol levels in canine plasma and platelets were measured. (biomedcentral.com)
CONCLUSIONS1
CONCLUSIONS: Our findings demonstrate a shift in PMF types among US coal miners, from a predominance of coal- and mixed-type PMF to a more commonly encountered silica-type PMF. (cdc.gov)
Gene1
In the present study we used Affymetrix oligonucleotide microarrays to produce gene transcription profiles for the major leukocyte types in humans. (gsea-msigdb.org)
Functions1
Part I: Enzymology, Molecular Biology, and Biological Functions of Mammalian Lipoxygenases. (edu.au)
Humans1
Exceptions to this rule include the 12R-lipoxygenases of humans and other mammals (see below). (wikipedia.org)
Significantly2
More recent year of birth was significantly associated with silica-type PMF. (cdc.gov)
These results also indicate that intravenously administered tocotrienols were significantly better than tocopherols in inhibiting cyclic flow reductions (CFRs), a measure of the acute platelet-mediated thrombus formation. (biomedcentral.com)
Human1
Description: Quantitativesandwich ELISA kit for measuring Human Arachidonate 15-lipoxygenase B (ALOX15B) in samples from serum, plasma, tissue homogenates, cell lysates. (myelisakit.com)
while the third domain of terrestrial life, the archaea, possesses proteins with a slight (~20%) amino acid sequence similarity to lipoxygenases, these proteins lack iron-binding residues and therefore are not projected to possess lipoxygenase activity. (wikipedia.org)
Sequence data is available for the following lipoxygenases: Plants express a variety of cytosolic lipoxygenases (EC 1.13.11.12InterPro: IPR001246) as well as what seems to be a chloroplast isozyme. (wikipedia.org)
Products1
Most lipoxygenases catalyze the formation of initially formed hydroperoxy products that have S chirality. (wikipedia.org)
Activity1
The lipoxygenases are related to each other based upon their similar genetic structure and dioxygenation activity. (wikipedia.org)
Activation2
Our results show that maternal dietary n-3 PUFA deficiency increases microglia-mediated phagocytosis of synaptic elements in the rodent developing hippocampus, partly through the activation of 12/15-lipoxygenase (LOX)/12-HETE signaling, altering neuronal morphology and affecting cognitive performance of the offspring. (nature.com)
This comprehensive dataset enabled us to not only establish which genes were expressed in each leukocyte type, but also which genes were expressed in each subset after activation. (gsea-msigdb.org)
Express1
Many cell types involved in this process express components of the cannabinoid signaling system that can be endogenously or pharmacologically controlled. (420magazine.com)
Found1
RESULTS: Of 322 cases found to have PMF, study pathologists characterised 138 (43%) as coal-type, 129 (40%) as mixed-type and 55 (17%) as silica-type PMF. (cdc.gov)
Cells1
Lipoxygenases depend on the availability of their polyunsaturated fatty acid substrates which, particularly in mammalian cells, is normally maintained at extremely low levels. (wikipedia.org)