Platelet adhesion platelet aggregation. Medical search. Frequent questions

We investigated, whether GPVI-Fc added in vitro on top of acetylsalicylic acid (ASA), the P2Y 12 antagonist ticagrelor, and the fibrinogen receptor antagonist abciximab alone or in combination would increase inhibition of platelet activation by atherosclerotic plaque. (thieme-connect.com)
Under static conditions, GPVI-Fc inhibited plaque-induced platelet aggregation by 53%, and increased platelet inhibition by ASA (51%) and ticagrelor (64%) to 66% and 80%, respectively. (thieme-connect.com)
Under arterial flow, GPVI-Fc inhibited plaque-induced platelet aggregation by 57%, and significantly increased platelet inhibition by ASA (28%) and ticagrelor (47%) to about 81% each. (thieme-connect.com)
In contrast, the strong inhibition of platelet adhesion by GPVI-Fc in combination with GPIIb/IIIa inhibitors could be harmful. (thieme-connect.com)
The mainstay of ACS treatment revolves around platelet inhibition. (jabfm.org)
Most of the developments in pharmacotherapy have been in targeting platelet inhibition because platelet aggregation and the resultant formation of platelet-rich thrombi are the primary events in the pathogenesis of ACS. (jabfm.org)
Thienopyridines are a group of drugs whose metabolites bind irreversibly to adenosine diphosphate (ADP) receptors on platelets (P2Y12 receptors), resulting in the inhibition of ADP-induced platelet activation and aggregation. (jabfm.org)
It has now been found that certain compounds within the scope of International Patent Application WO 9905143 but not specifically disclosed therein exhibit high potency combined with surprisingly high metabolic stability and bioavailibility, such that the predicted therapeutic dose for prolonged inhibition of aggregation in man shows advantage. (justia.com)
We also looked at the reactivity of platelets in their storage milieu and, indeed, found there was already inhibition of platelet aggregation in response to ADP on Day 1.5, and to collagen on Day 6.5, which was, however, similar between the untreated and the IBS-PCs 2 (see Online Supplementary Figure S2B). (haematologica.org)

GELITA-CEL ® products induce hemostasis by providing a strong matrix for platelet adhesion and aggregation. (southmedic.com)

Figure 2: Platelet aggregation induced shear microgradients occurs independently of ADP, TXA 2 and thrombin. (nature.com)
This initial interaction (platelet adhesion) sets the stage for other adhesive reactions that allow the platelets to interact with other agonists in the vicinity of vessel injury, such as adenosine 5'-diphosphate (ADP), subendothelial collagen, and thrombin. (medscape.com)
Platelets were activated with ADP, thrombin, or the thrombin receptor agonist peptide SFLLRN (TRAP 1-6 ). (nature.com)
Thrombin can produce platelet aggregation largely independently of other pathways but substantial quantities of thrombin are unlikely to be present without prior activation of platelets by other mechanisms. (justia.com)
Platelets are activated when brought into contact with collagen (which is exposed when the endothelial blood vessel lining is damaged), thrombin (primarily through PAR -1), ADP receptors (P2Y1 and P2Y12) expressed on platelets, a negatively charged surface (e.g. glass), or several other activating factors. (wikidoc.org)
This study provides results of early diminished platelet function in IBS-treated PCs as compared to conventional PCs, i.e., reduced aggregation response to collagen or thrombin and adhesion to collagen or vWF under flow, increased platelet apoptosis, MAPK p38 activation, and glycoprotein Ibα (GPIbα) shedding and enhanced clearance from the circulation of mice. (haematologica.org)
Indeed, we showed that washed IBS-platelets were fully responsive to various agonists including collagen, thrombin, and the so-called weak platelet agonist ADP up to Day 4.5, while a slight decline in responsiveness was observed on Day 6.5, which was, however, exactly the same in washed IBS-platelets as washed untreated platelets, whatever the agonist. (haematologica.org)
Thrombin converts fibrinogen into fibrin monomers, and the fibrin monomers polymerize into fibrin polymers that bind aggregated platelets into platelet-fibrin hemostatic plugs. (msdmanuals.com)
Coagulation factors interact on platelet and endothelial cell surfaces to produce thrombin , which converts fibrinogen to fibrin. (msdmanuals.com)

Adhesion receptors promote the adhesion of platelets to other platelets, the vessel wall or leucocytes , depending on the receptor stimulated. (teachmephysiology.com)
Once activated, platelets express the GPIIb/IIIa receptor which can then bind with vWF or fibrinogen. (teachmephysiology.com)
Savage, B., Almus-Jacobs, F. & Ruggeri, Z.M. Specific synergy of multiple substrate-receptor interactions in platelet thrombus formation under flow. (nature.com)
Shear-Mediated Platelet Microparticles Demonstrate Phenotypic Heterogeneity as to Morphology, Receptor Distribution, and Hemostatic Function. (harvard.edu)
Sex-specific platelet activation through protease-activated receptor-1 in patients undergoing cardiac catheterization. (harvard.edu)
GPVI-Fc added on top of abciximab, a clinically used anti-fibrinogen receptor antibody which blocks platelet aggregation, strongly inhibited total (81%) and stable (89%) platelet adhesion. (thieme-connect.com)
P-selectin, soluble endothelial protein C receptor, soluble thrombomodulin, plasminogen activator inhibitor 1, ADAMTS-13, von Willebrand factor, tissue factor, soluble intercellular adhesion molecule 1, and vascular cell adhesion molecule 1 were more elevated in LF patients than in controls. (cdc.gov)
Endothelial protein C receptor, thrombomodulin, intercellular adhesion molecule 1, plasminogen activator inhibitor 1, D-dimer, and hepatocyte growth factor were higher in fatal than nonfatal LF cases. (cdc.gov)
Betahistine hydrochloride is a histamine H1 receptor agonist that can resist platelet aggregation. (ijpsonline.com)
Betahistine hydrochloride is a Histamine (H 1 ) receptor agonist that can resist platelet aggregation. (ijpsonline.com)
Available antiplatelet agents, such as cyclooxygenase-1 (COX-1) inhibitors (aspirin), ADP P2Y 12 receptor antagonists, and GP IIb/IIIa receptor inhibitors, are effective and save in the treatment and prevention of thrombotic events, these drugs interfere with the platelet activation process, including adhesion, release, and aggregation. (hindawi.com)
ADP-induced platelet aggregation is mediated by the P 2T receptor subtype located on the platelet membrane. (justia.com)
The P 2T receptor (also known as P2Y ADP or P2T AC ) is primarily involved in mediating platelet aggregation/activation and is a G-protein coupled receptor which is as yet uncloned. (justia.com)
Platelet aggregation is stimulated by thromboxane and α2 receptor -activation, but inhibited by other inflammatory products like PGI2 and PGD2 . (wikidoc.org)
A nitric oxide (NO) detection kit was used to determine the concentration of NO. Western blotting analysis was performed to determine the levels of intercellular cell adhesion molecule (ICAM)‑1, vascular cell adhesion molecule (VCAM)‑1, endothelin‑1, caspase‑3, Bax, Bcl‑2, Toll‑like receptor 4 (TLR4), p65 and p‑p65. (spandidos-publications.com)
Published in the prestigious journal Nature Materials , the researchers demonstrated blood flow disturbance could lead to previously unrecognised intermediate activation of integrin, an adhesion receptor that mediates platelet aggregation to form a clot. (edu.au)
Platelet receptors for ADP include the P2Y12 receptor, which sends signals to suppress adenylate cyclase, decreases cyclic adenosine monophosphate (cAMP) levels, and promotes activation of the glycoprotein IIb/IIIa receptor (assembled on the activated platelet surface membrane from glycoproteins IIb and IIIa). (msdmanuals.com)

The irreversible binding to the receptors makes these drugs capable of inhibiting platelet function for the life span of platelets (5-7 days). (jabfm.org)

In fact, in addition to their potent vasodilating effect, EETs have potent anti-inflammatory properties, inhibit platelet aggregation, promote fibrinolysis, and reduce vascular smooth muscle cell proliferation. (frontiersin.org)
These drugs, unlike thienopyridines, bind reversibly, but directly without biotransformation, to P2Y12 receptors on platelets and hence inhibit platelet function. (jabfm.org)
We reasoned that the presence of prostacyclin (PGI 2 ) during the washing procedure to inhibit platelet activation and of apyrase in the suspending milieu to degrade trace amounts of ADP released from platelets and thus prevent the desensitization of the ADP receptors, might mimic the inhibitory role of the vascular endothelium, known to express ectonucleotidase activities and to produce PGI 2 , the natural strong vasodilator and inhibitor of platelet activation. (haematologica.org)

Exposed collagen fibres bind vWF released from the damaged endothelium, which in turn binds to vWF receptors on platelets to promote adhesion. (teachmephysiology.com)
The exposed collagen itself also promotes platelet binding. (teachmephysiology.com)
When a platelet binds to collagen, the glycoprotein IIb/IIIa pathway is activated - a complex system controlled by G-protein coupled receptors (GPCRs). (teachmephysiology.com)
GPVI-Fc, a recombinant dimeric fusion protein binding to plaque collagen and concealing binding sites for platelet GPVI, acts as a lesion-focused antiplatelet drug, and does not increase bleeding in vivo. (thieme-connect.com)

In Sierra Leone during 2015-2018, we assessed LF patients' day-of-admission plasma samples for levels of proteins necessary for coagulation, fibrinolysis, and platelet function. (cdc.gov)
Vascular endothelial cells serve a key role in the pathogenesis of atherosclerosis by regulating vascular tension, platelet adhesion, inflammation and fibrinolysis ( 5 ). (spandidos-publications.com)

The production of the platelet plug is an example of positive feedback and thus it is necessary to have measures in place to prevent excessive and inappropriate clot formation. (teachmephysiology.com)
During clot retraction, the platelets within the clot contract, causing the fibrin strands to pull together and shrink the clot. (proprofs.com)
Prostacyclin is a vasodilator and inhibits platelet aggregation, therefore it would actually prevent clot formation. (proprofs.com)
This accelerates the formation of a platelet plug and acts as a catalyst in forming a fibrin clot. (southmedic.com)
platelets and fibrin combine to form a clot. (msdmanuals.com)

It also inhibits platelet aggregation, induces disaggregation of aggregated platelets, and inhibits platelet adhesion to the vascular endothelium. (bvsalud.org)

CD11a/CD18 (LFA-1) expressed on lymphocytes is known to play an important role in lymphocyte trafficking (adhesion to vascular endothelium), as well as interactions to antigen presenting cells (APC). (medscape.com)
ADP, thromboxane A2, and other mediators induce activation and aggregation of additional platelets on the injured endothelium. (msdmanuals.com)

It is characterized by thrombocytopenia with macrothrombocytes in 30% of cases and variable platelet aggregation in response to adenosine diphosphate, depending on the platelet count. (msdvetmanual.com)
During activation, platelets release mediators of aggregation, including adenosine diphosphate (ADP) from storage granules. (msdmanuals.com)

Fibrinogen facilitates the formation of crosslinks between platelets, aiding platelet aggregation to form a platelet plug. (teachmephysiology.com)
Savage, B., Saldivar, E. & Ruggeri, Z.M. Initiation of platelet adhesion by arrest onto fibrinogen or translocation on von Willebrand factor. (nature.com)
On resting platelets, GP IIb/IIIa is unable to bind fibrinogen or vWf. (medscape.com)
The alpha granules contain hemostatic proteins such as fibrinogen, vWf, and growth factors (eg, platelet-derived growth factor and transforming growth factors). (medscape.com)
CD41/CD61 is expressed on platelets and megakaryocytes, and plays a role in platelet activation and aggregation through interaction with fibrinogen, fibronectin, vWF, and other RGD-containing adhesion molecules. (biolegend.com)
Whatever the initial stimulus, the final common event is a cross-linking of platelets by binding of fibrinogen to a membrane-binding site, glycoprotein IIb/IIIa (GPIIb/IIIa). (justia.com)
Fibrinogen binds to the glycoprotein IIb/IIIa complexes of adjacent platelets, connecting them into aggregates. (msdmanuals.com)

Peripheral versus central venous blood sampling does not influence the assessment of platelet activation in cirrhosis. (harvard.edu)

The hemostatic system consists of platelets, coagulation factors, and the endothelial cells lining the blood vessels. (medscape.com)
Under physiological circumstances, the resistance of the endothelial cell lining to interactions with platelets and coagulation factors prevents thrombosis. (medscape.com)
Once activated, they release a number of different coagulation factors and platelet activating factors. (wikidoc.org)

Thienopyridines are converted to active metabolites ( Figure 1 ) that bind irreversibly to P2Y12 receptors on platelets and thereby inhibit ADP-induced platelet activation and aggregation. (jabfm.org)

Platelet aggregation at sites of vascular injury is essential for hemostasis and arterial thrombosis. (nature.com)
Platelet disorders lead to defects in primary hemostasis and produce signs and symptoms different from coagulation factor deficiencies (disorders of secondary hemostasis). (medscape.com)
The initial hemostatic plug, composed primarily of platelets, is stabilized further by a fibrin mesh generated in secondary hemostasis. (medscape.com)
Platelets , or thrombocytes , are the cells circulating in the blood that are involved in the cellular mechanisms of primary hemostasis leading to the formation of blood clots . (wikidoc.org)

The platelet GP IIb/IIIa complex mediates platelet-to-platelet interactions (platelet aggregation). (medscape.com)

Thrombocytopenia is a common feature of hemorrhagic fevers and vascular permeability disorders ( 8 ), but the decrease in platelet counts in acute LF is not low enough to cause spontaneous hemorrhage. (cdc.gov)
The workup of a patient with a platelet-type bleeding pattern typically includes correlation with platelet count ( thrombocytopenia ), evaluation of platelet morphology on peripheral smear, exclusion of acquired causes of platelet dysfunction, and testing for von Willebrand disease (VWD) . (arupconsult.com)
Disorders of platelets can be divided into acquired or congenital thrombocytopenias and acquired or congenital functional disorders (thrombocytopathias), with acquired thrombocytopenia being the most common. (msdvetmanual.com)
Fetal and neonatal alloimmune thrombocytopenia is a rare disorder that occurs when maternal antibodies are produced against a paternal antigen on fetal platelets. (msdvetmanual.com)

Leukocyte adhesion deficiency (LAD) is a rare primary immunodeficiency. (medscape.com)
Thus the infections in patients with leukocyte adhesion deficiency act similarly as those observed in patients with neutropenia. (medscape.com)
Labial ulceration from which Escherichia coli was cultured in an 8-month-old girl with leukocyte adhesion deficiency type 1 (LAD I). Note the thin bluish scar at the superior aspect of the labia from an earlier cellulitis. (medscape.com)
This 3-year-old girl had leukocyte adhesion deficiency type I (LAD I) with complete absence of CD18 expression. (medscape.com)
This 10-month-old patient with severe leukocyte adhesion deficiency type I (LAD I) developed a cervical adenitis caused by Klebsiella pneumoniae. (medscape.com)
Leukocyte adhesion deficiency type I (LAD I) is a failure to express CD18, which composes the common ß 2 subunit of LFA1 family (ß2 integrins). (medscape.com)
In milder forms of leukocyte adhesion deficiency I (1-30% expression of CD8), patients may survive to adulthood. (medscape.com)
Patients with leukocyte adhesion deficiency II manifest the Bombay phenotype (ie, negative for O and H blood group antigens with potential production of anti-H antibody). (medscape.com)
However, IgM and IgG serum levels are within the reference range in patients with leukocyte adhesion deficiency II. (medscape.com)
Leukocyte adhesion deficiency II may be classified as one of the congenital disorders of glycosylation (CDG), a rapidly expanding group of metabolic syndromes with a wide symptomatology and severity. (medscape.com)
Currently, 18 subtypes have been reported: 12 are type I (dysfunctional lipid-linked oligosaccharide precursor synthesis), and 6 are type II (dysfunctional trimming/processing of the protein-bound oligosaccharide), including leukocyte adhesion deficiency II (CDG-IIc). (medscape.com)
Variants of leukocyte adhesion deficiency have also been reported, including fully expressed but nonfunctional CD18 and an E selectin that is expressed but rapidly cleaved from the cell surface (only present in soluble form). (medscape.com)
Another reported type of leukocyte adhesion deficiency involves dysfunction in platelet aggregation in addition to a defect in leukocyte adhesion. (medscape.com)
Thus, patients with this type of leukocyte adhesion deficiency manifest both severe bacterial infections and bleeding disorder. (medscape.com)
This leukocyte adhesion deficiency variant is associated with defective expression of the Rap-1 activator CalDAG-GEFI. (medscape.com)

Platelet activation results in a morphological change on the membrane surface of the platelet, increasing the surface area and preparing it for aggregation. (teachmephysiology.com)
Platelet activation allows binding of these proteins, which bridges adjacent platelets. (medscape.com)
Platelet activation pathways. (medscape.com)
Dematin Regulates Calcium Mobilization, Thrombosis, and Early Akt Activation in Platelets. (harvard.edu)
Platelet Activation and Reactivity in a Large Cohort of Patients with Gaucher Disease. (harvard.edu)
Platelet activation and aggregation plays an integral role in the pathogenesis of acute coronary syndrome (ACS). (jabfm.org)
Stehbens and Biscoe, 1967 ), platelet shape change was, and still is, generally regarded to be the initial stage of platelet activation. (nature.com)
MPV is considered a marker of platelet function and activation: larger platelets are more reactive and aggregable. (hindawi.com)
Functional platelet disorders affect the adhesion, activation, or aggregation of platelets and are frequently associated with excessive bleeding, often following trauma or surgery. (arupconsult.com)
The contents of the granules are released upon activation of the platelet. (wikidoc.org)
Platelet activation further results in the scramblase -mediated transport of negatively charged phospholipids to the platelet surface. (wikidoc.org)
Finally, IBS did not substantially alter platelet proteome as could be evaluated by the 2D-DIGE technology, indicating overall that IBS did not induce any clear intrinsic defect in integrity, function or increased spontaneous activation of platelets isolated from the storage milieu. (haematologica.org)
While coagulation - the activation of platelets clumping together - is key to stopping blood loss from a cut or wound, overactivation can lead to deadly blood clots, heart attack or stroke. (edu.au)
Researchers have used a microfluidic channel, mimicking the narrowing of vessels that cause blood clots, to observe the activation of platelets at the single-molecular level. (edu.au)
Although these drugs inhibit the biochemical activation of platelets, they may not block the platelet biomechanical signaling pathways. (edu.au)
For a long time, studies in the field of thrombosis have attempted to understand the mechanism of platelet activation at the cellular and molecular levels, and hope to provide ideas for the development of new antithrombotic drugs with strong efficacy and few side effects. (edu.au)
The 'mechanically driven platelet activation' phenomenon found in the study explains why platelets can aggregate and accumulate by mechanical stimulation of high-speed turbulence alone, leading to blockage of a blood vessel. (edu.au)
The activation capacity of 2bFXa-HB platelets remained unchanged, and there were no signs of elevated thrombotic risk in these mice. (bvsalud.org)
In an acute alcohol exposure mouse model, a single administration of alcohol reduced both the number of platelets and their activation capacity, as well as impaired coagulation function. (bvsalud.org)
In the past, the role of different proteins in platelet adhesion, activation, and aggregate formation has been analyzed using platelets/mice with a genetic loss of a certain protein. (bvsalud.org)
Platelets anchored to the vessel wall undergo activation. (msdmanuals.com)

Once activated, platelets have two major mechanisms to recruit additional platelets to the growing hemostatic plug. (medscape.com)

The impaired homeostasis and platelet dysfunction implicate alterations in the protein C pathway, which might contribute to the loss of endothelial barrier function in fatal infections. (cdc.gov)
Factors that may contribute to this greater platelet reactivity are not completely elucidated and include metabolic abnormalities as hyperglycemia, hyperlipidemia, insulin resistance, and conditions as oxidative stress, inflammation, and endothelial dysfunction [ 3 ]. (hindawi.com)
Dysfunction or low levels of platelets predisposes to bleeding , while high levels, although usually asymptomatic, may increase the risk of thrombosis . (wikidoc.org)

It is a member of the integrin family, associating with platelet gpIIb (CD41) to form CD41/CD61 complex and with integrin aV (CD51) to form aV/ß3 (CD51/CD61) integrin. (biolegend.com)
Platelet Glycoprotein GPIIb-IIIa Complex" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
This graph shows the total number of publications written about "Platelet Glycoprotein GPIIb-IIIa Complex" by people in Harvard Catalyst Profiles by year, and whether "Platelet Glycoprotein GPIIb-IIIa Complex" was a major or minor topic of these publication. (harvard.edu)
Below are the most recent publications written about "Platelet Glycoprotein GPIIb-IIIa Complex" by people in Profiles. (harvard.edu)
The high anti-platelet efficacy of antibodies or antagonists for GPIIb/IIIa is explained by their interference with this final common event. (justia.com)

The ability of platelets to carry out their hemostatic function can be impaired in a wide range of inherited and acquired conditions: trauma, surgery, inflammation, pre-term birth, sepsis, hematological malignancies, solid tumors, chemotherapy, autoimmune disorders, and many others. (nih.gov)

In addition, when platelets are activated, negatively charged phospholipids move from the inner to the outer leaflet of the membrane bilayer. (medscape.com)

It has long been assumed that platelet aggregation and thrombus growth are initiated by soluble agonists generated at sites of vascular injury. (nature.com)
These findings provide insight into the prothrombotic effects of disturbed blood flow parameters and suggest a fundamental reinterpretation of the mechanisms driving platelet aggregation and thrombus growth. (nature.com)
Figure 3: Stabilized discoid platelet aggregation is a general feature of thrombus development. (nature.com)
Intercellular calcium communication regulates platelet aggregation and thrombus growth. (nature.com)
Human atheromatous plaques stimulate thrombus formation by activating platelet glycoprotein VI. (thieme-connect.com)
Platelets are main drivers of thrombus formation. (bvsalud.org)
Besides platelet aggregate formation, platelets interact with different blood cells such as red blood and white blood cells (RBCs, WBCs) and endothelial cells (ECs), to promote thrombus formation and inflammation. (bvsalud.org)
Besides, the interaction of platelets with RBCs, WBCs, and ECs under flow conditions has been evaluated with regard to thrombus formation and platelet-mediated inflammation. (bvsalud.org)

Platelets originate from megakaryocytes, which are the largest progenitor cells of the bone marrow. (teachmephysiology.com)
The platelets arise from the fragmentation of the cytoplasm of megakaryocytes in the bone marrow and circulate in blood as disc-shaped anucleate particles for 7-10 days. (medscape.com)
Platelets are produced in hematopoiesis by budding off from megakaryocytes . (wikidoc.org)

Platelets from subjects with DM, particularly from those with type 2 diabetes, exhibit increased reactivity. (hindawi.com)
The preserved reactivity of IBS-platelets was further confirmed in perfusion studies over adhesive protein-coated surfaces under relevant shear rates, in accordance with previously reported results. (haematologica.org)

The normal platelet count is 150-400 x 10 9 /L. Although they are primarily found in the bloodstream, up to 30% of platelets are transiently sequestered within the spleen , ready for rapid mobilisation. (teachmephysiology.com)
A normal platelet count in a healthy person is between 150,000 and 400,000 per mm³ of blood (150-400 x 10 9 / L ). 95% of healthy people will have platelet counts in this range. (wikidoc.org)

A number of converging pathways lead to platelet aggregation. (justia.com)

further testing may include platelet aggregation tests, platelet flow cytometry, and in some cases, genetic testing. (arupconsult.com)

Thus, the release reaction and prostaglandin synthesis act to consolidate the initial hemostatic plug by promoting the participation of other platelets in the growing hemostatic plug. (medscape.com)

4 Among these, colchicine is able to impair platelet aggregation and leucocyte migration through an action on adhesion molecules, 5 effect shared with NSAIDs, including salicylates. (bmj.com)

An activated human platelet - a blood clotting cell that plays a crucial role in preventing bleeding or causing the cardiovascular diseases such as heart attack and stroke. (edu.au)

The result is the secretion of ADP and thromboxane A2 which subsequently activate other platelets. (teachmephysiology.com)
They contain denser granules, secrete more serotonin and β -thromboglobulin, produce more thromboxane A2 and have more adhesion molecules (like P-selectin and platelet glycoprotein-GP-IIbIIIa), than smaller platelets. (hindawi.com)
The limited anti-thrombotic efficacy of aspirin may reflect the fact that it blocks only one source of ADP which is that released in a thromboxane-dependent manner following platelet adhesion (see e.g. (justia.com)

Platelet disaggregation occurred only in samples from fatal LF cases. (cdc.gov)

to Platelet adhesion and aggregation are initiating events in arterial thrombosis. (justia.com)

Distinct glycoprotein Ib/V/IX and integrin αIIbβ3-dependent calcium signals cooperatively regulate platelet adhesion under flow. (nature.com)
Microaggregation appeared to be distinct from full aggregation, as it was not inhibited by the addition of CD61, an antibody to the β 3 integrin. (nature.com)
Integrin is a mechanosensory protein that platelets use to sense mechanical force generated by dynamic blood flow. (edu.au)
The integrin can instruct the platelet cells' clotting behaviours. (edu.au)

Platelets play a primary role in this process, interacting with subendothelium-bound von Willebrand factor (vWf) via the membrane glycoprotein (GP) Ib complex. (medscape.com)
Platelet membrane glycoprotein complex important for platelet adhesion and aggregation. (harvard.edu)
VWF binds to receptors on the platelet surface membrane (glycoprotein Ib/IX). (msdmanuals.com)

Initial platelet adhesion is to long strings of von Willebrand factor (VWF) that have been previously secreted by, and anchored to, stimulated endothelial cells. (msdmanuals.com)

The body's reaction to vessel wall injury is rapid adhesion of platelets to the subendothelium. (medscape.com)

It was suggested that this optical effect was caused by the morphological change of platelets from discs to spherical cells with pseudopodia ( Latimer et al, 1977 ). (nature.com)

Analysis of blood flow dynamics revealed that discoid platelets preferentially adhere in low-shear zones at the downstream face of forming thrombi, with stabilization of aggregates dependent on the dynamic restructuring of membrane tethers. (nature.com)
The platelets adhere to each other via adhesion receptors or integrins , and to the endothelial cells in the wall of the blood vessel forming a haemostatic plug in conjunction with fibrin . (wikidoc.org)
Under these conditions, platelets adhere to the damaged intima and form aggregates. (msdmanuals.com)

By using high-resolution intravital imaging techniques and hydrodynamic analyses, we show that platelet aggregation is primarily driven by changes in blood flow parameters (rheology), with soluble agonists having a secondary role, stabilizing formed aggregates. (nature.com)
Platelet aggregometry is a standard clinical technique that measures the in vitro aggregability of platelets in response to agonists by using the amount of light transmitted through platelet-rich plasma (PRP) ( Holmsen, 1987 ). (nature.com)
Severe abnormalities in platelet function, such as Bernard-Soulier syndrome, Glanzmann's thrombasthenia, or platelet storage-pool deficiency, are characterized by the inability of platelets to aggregate in response to certain agonists. (nature.com)

How do functional platelet disorders manifest clinically? (arupconsult.com)
The clinical features of functional platelet disorders vary. (arupconsult.com)
rarely, it may be observed in functional platelet disorders). (arupconsult.com)
Functional platelet disorders are generally associated with immediate bleeding in response to surgery or trauma, whereas factor deficiencies are associated with delayed bleeding. (arupconsult.com)
What are some causes of acquired functional platelet disorders? (arupconsult.com)
Which functional platelet disorders are inherited? (arupconsult.com)
Bernard-Soulier syndrome (an adhesion disorder) and Glanzmann thrombasthenia (an aggregation disorder) are among the most well-recognized inherited disorders of platelet function and are severe clinical conditions. (arupconsult.com)
Refer to the table, Laboratory Findings in Selected Inherited Functional Platelet Disorders , for additional information and a more comprehensive list of inherited functional platelet disorders. (arupconsult.com)
Laboratory testing for functional platelet disorders is appropriate in individuals with bleeding manifestations that suggest a functional platelet disorder (eg, easy bruising, prolonged bleeding following dental work or surgery, menorrhagia), particularly those with a family history of a bleeding disorder and in whom VWD has been previously excluded. (arupconsult.com)

70%. Severe cases exhibit abnormal coagulation, endothelial barrier disruption, and dysfunctional platelet aggregation but the underlying mechanisms remain poorly understood. (cdc.gov)
Various mechanisms, including endothelial cell nitric oxide and prostacyclin, promote blood fluidity by preventing platelet aggregation and dilating intact blood vessels. (msdmanuals.com)

Platelets have abundant surface receptors , classified into agonist and adhesion receptors. (teachmephysiology.com)
This analysis is performed for both resting and dual-agonist-stimulated platelets. (nih.gov)

We created transgenic mice called 2bFXa-HB, which had stable expression and storage of FXa in their platelets, resulting in the alleviation of the bleeding disorder in these mice. (bvsalud.org)

The method output includes levels of forward and side scatter, levels of major adhesion and aggregation glycoproteins Ib and IIb-IIIa, active integrins' level based on PAC-1 binding, major alpha-granule component P-selectin, dense granule function based on mepacrine uptake and release, and procoagulant activity quantified as a percentage of annexin V-positive platelets. (nih.gov)
The antigenic target in most patients appears to be the platelet GP IIb/IIIa complex. (medscape.com)

Figure 1: Shear microgradients promote platelet aggregate formation in vivo . (nature.com)

GPVI-Fc alone or in combination with ASA or ticagrelor did not increase closure time measured by the platelet function analyzer (PFA)-200. (thieme-connect.com)

hence, focus in drug development has been to create more potent inhibitors of platelet aggregation. (jabfm.org)
This was also seen when preactivated platelets, which cannot undergo further morphological changes, were used, and when platelets were activated in the presence of shape-change inhibitors such as cytochalasin D and vincristine. (nature.com)

In this article, we will consider the structure, function and clinical relevance of platelets. (teachmephysiology.com)
Trpc6 gain-of-function disease mutation enhances phosphatidylserine exposure in murine platelets. (harvard.edu)
aspirin and nonsteroidal anti-inflammatory drugs (NSAIDs) are common causes of abnormal platelet function, and platelet function testing may need to be deferred if patients have been taking these medications. (arupconsult.com)

We conclude that platelet aggregation occurs independently of shape change and that shape change is not necessarily followed by aggregation. (nature.com)
1 and others 6 4 is the fact that we isolated platelets from the storage milieu in order to explore their intrinsic functional properties, independently of the storage milieu which may have an inhibitory-yet-reversible effect on platelet responsiveness. (haematologica.org)
Of note, independently of IBS, we showed that the level of spontaneous P-selectin expression on platelets kept in their storage milieu resulted in approximately 30% positive cells at Day 1.5, which is the lowest amount reported by others. (haematologica.org)

We find that in response to vascular injury, thrombi initially develop through the progressive stabilization of discoid platelet aggregates. (nature.com)
Figure 5: Stabilized discoid platelet aggregation occurs via restructuring of membrane tethers. (nature.com)

Preliminary work suggests that policosanol is an effective platelet aggregation inhibitor. (digitalnaturopath.com)

Anatomy21

Organisms7

Diseases17

Chemicals and Drugs137

Analytical, Diagnostic and Therapeutic Techniques and Equipment31

Phenomena and Processes48

Disciplines and Occupations1

Technology, Industry, Agriculture5

Information Science4

Inhibition9

Matrix for platelet adhesion and aggregation1

Thrombin9

Receptor17

Inhibiting platelet function1

Inhibit platelet3

Collagen4

Fibrinolysis2

Clot5

Inhibits platelet aggregation1

Endothelium2

Adenosine diphosphate2

Fibrinogen7

Assessment of platelet1

Coagulation factors3

Receptors on platelets1

Hemostasis4

Mediates1

Thrombocytopenia4

Leukocyte adhesion15

Activation21

Additional platelets1

Dysfunction3

GPIIb5

Ability of platelets1

Negatively charged phospholipids1

Thrombus8

Megakaryocytes3

Reactivity2

Normal platelet2

Pathways1

Flow Cytometry1

Hemostatic1

Leucocyte1

Blood Clotting1

Thromboxane3

Disaggregation1

Thrombosis1

Integrin4

Membrane glycoprotein3

Willebrand1

Vessel wall1

Morphological change1

Adhere3

Agonists3

Disorders9

Mechanisms2

Agonist2

Bleeding disorder1

IIIa2

Aggregate formation1

GPVI1

Inhibitors2

Function3

Independently3

Discoid2

Inhibitor1