Including 12-hArachidonate 12-lipoxygenInhibitionLipidsAggregationPathwayArachidonic AcidNeutrophilsInflammationInhibitCytochromeMetabolitesCyclooxygenasesEndothelialInhibitorEnzymesPhospholipaseCoronaryMammalianAcutePathwaysCollagenInhibitorsPhosphodiesteraseVivoDocosahexaenoic acidPromotesClinicalMyocardialProteinOxylipinsGeneFatty acidsLungProcessesMolecularAdultsMechanismsErythrocytesBloodPotentCognitiveEffectResearchPlasma
Including 12-h1
- Lipoxygenases (LOX) contribute to vascular disease and inflammation through generation of bioactive lipids, including 12-hydro(pero xyeicosatetraenoic acid (12-H(P)ETE). (reading.ac.uk)
Arachidonate 12-lipoxygen3
- Arachidonate 12-Lipoxygenase" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
- This graph shows the total number of publications written about "Arachidonate 12-Lipoxygenase" by people in this website by year, and whether "Arachidonate 12-Lipoxygenase" was a major or minor topic of these publications. (umassmed.edu)
- Below are the most recent publications written about "Arachidonate 12-Lipoxygenase" by people in Profiles. (umassmed.edu)
Inhibition4
- In contrast, protein kinase C (PKC) inhibition potentiated 12-H(P)ETE generation. (reading.ac.uk)
- The inhibitory effects of α-tocopherol on platelet function in vivo and ex vivo have been well established [ 14 - 18 ], but inhibition of platelet aggregation by α-tocopherol in vitro requires non-physiologically high levels of α-tocopherol [ 14 ]. (biomedcentral.com)
- The ACKR3/CXCR7 ligation coordinated with the Gs-coupled prostacyclin receptor to cause platelet inhibition via cyclic adenosine monophosphate/protein kinase A. Ligation of the ACKR3/CXCR7 receptor lowered the production of lipid agonists and signaling intermediates, affecting calcium mobilization, intracellular signaling, and, as a result, platelet interaction with physiological matrices and the thrombo inflammatory secretome. (physiciansweekly.com)
- The synthesized compounds were evaluated for dual cyclooxygenases (COX) and lipoxygenase (LOX) inhibition. (technoplus.ro)
Lipids4
- a -Hydroxy acids or 2-hydroxy acids are found in plants (chain from 12 up to 24 carbon atoms) and in animal wool waxes, skin lipids and specialized tissues, mainly in brain. (gerli.com)
- Both reduce blood viscosity without a significant effect on platelet or clotting factors, and both have positive effects on blood lipids, most consistently a reduction in triglyceride concentrations [ 9 ]. (biomedcentral.com)
- In healthy subjects, ACKR3/CXCR7 ligation favored the generation of antithrombotic lipids (dihomo-linolenic acid [DGLA], 12-hydroxyeicosatrienoic acid [12-HETrE]) over COX-1 or 12-lipoxygenase (12-LOX) metabolized prothrombotic and phospholipase-derived atherogenic lipids. (physiciansweekly.com)
- In addition, ex vivo, CXCR7 agonist regulated heparin-induced thrombocytopenia-sera/immunoglobulin G-triggered platelet and neutrophil activation, heparin-induced platelet aggregation, generation of thrombo inflammatory lipids, platelet-neutrophil aggregate formation, and thrombo inflammatory secretions. (physiciansweekly.com)
Aggregation4
- Our objective in this study was to determine the extent to which tocotrienols inhibit platelet aggregation and reduce coronary thrombosis, a major risk factor for stroke in humans. (biomedcentral.com)
- a mixture of α- + γ- + δ-tocotrienols) on in vivo platelet thrombosis and ex vivo platelet aggregation (PA) after intravenous injection in anesthetized dogs, by using a mechanically stenosed circumflex coronary artery model (Folts' cyclic flow model). (biomedcentral.com)
- The present results indicate that intravenously administered tocotrienols inhibited acute platelet-mediated thrombus formation, and collagen and ADP-induced platelet aggregation. (biomedcentral.com)
- Patients with CAD who had higher platelet ACKR3/CXCR7 expression had lower aggregation. (physiciansweekly.com)
Pathway4
- This study characterizes a receptor-dependent pathway for 12-H(P)ETE synthesis via the collagen receptor GPVI, which is negatively regulated by PECAM-1 and PKC, and demonstrates a novel link between immune receptor signaling and lipid mediator generation in the vasculature. (reading.ac.uk)
- Enteroaggregative Escherichia coli promotes transepithelial migration of neutrophils through a conserved 12-lipoxygenase pathway. (umassmed.edu)
- Effect of 15-HETE on the 5-Lipoxygenase Pathway in Neutrophils: Genuine Inhibitor or Alternative Substrate? (edu.au)
- The findings, published in the Jan. 1 online issue of Cancer Research,demonstrated dietary sugar's effect on an enzymatic signaling pathway known as 12-LOX (12-lipoxygenase). (mdanderson.org)
Arachidonic Acid5
- An enzyme that catalyzes the oxidation of arachidonic acid to yield 12-hydroperoxyarachidonate (12-HPETE) which is itself rapidly converted by a peroxidase to 12-hydroxy-5,8,10,14-eicosatetraenoate (12-HETE). (umassmed.edu)
- 12-Lipoxygenase plays a key role in cell death caused by glutathione depletion and arachidonic acid in rat oligodendrocytes. (umassmed.edu)
- previously named ML3000) is a substrate analogue of arachidonic acid which inhibits cyclooxygenase type 1 and 2 (COX1 and COX2) and 5-lipoxygenase (5-LOX), decreasing prostaglandins and leukotrienes production. (j-epilepsy.org)
- When one eats foods like meat or egg yolk, excess levels of arachidonic acid accumulate that is then broken down into toxic byproducts by the enzymes cyclooxygenase-2 (COX-2) and lipoxygenase (LOX). (lifeextension.com)
- In general, both EPA and DHA compete with arachidonic acid (omega-6) in cyclooxygenase and lipoxygenase synthesis, resulting in net anti-inflammatory effects. (biomedcentral.com)
Neutrophils3
- We observed that MPs and their cargo are internalized by activated neutrophils in the endomembrane system via 12(S)-HETE. (evtrack.org)
- Platelet MPs are found inside neutrophils isolated from the joints of arthritic patients, and are found in neutrophils only in the presence of sPLA2-IIA and 12-LO in an in vivo model of autoimmune inflammatory arthritis. (evtrack.org)
- These findings identify 12(S)-HETE as a trigger of platelet MP internalization by neutrophils, a mechanism highly relevant to inflammatory processes. (evtrack.org)
Inflammation3
- Systemic disease during Streptococcus pneumoniae acute lung infection requires 12-lipoxygenase-dependent inflammation. (umassmed.edu)
- Because sPLA2-IIA is induced during inflammation, and 12-LO expression is restricted mainly to platelets, these observations demonstrate that platelet MPs promote their internalization in recipient cells through highly regulated mechanisms. (evtrack.org)
- Therefore, with the aim to summarize the current knowledge on the initiation of the atherosclerotic process, in this paper, we review the early markers of atherosclerosis and we address the main therapeutic targets for preventing atheroma formation at its very initial stages focusing on inflammation, oxidative stress, endothelial dysfunction, and the interaction between platelets and endothelium. (hindawi.com)
Inhibit1
- ACKR3/CXCR7 ligation had no effect on surface receptor availability, coagulation profile, bleeding time, plasma-dependent thrombin generation (thrombinoscopy), or clot formation (thromboelastography), but it did inhibit activation-induced phosphatidylserine exposure and procoagulant platelet-assisted thrombin generation. (physiciansweekly.com)
Cytochrome2
- Changes in dietary fatty acids, specifically the polyunsaturated fatty acids of the ω-3 and ω-6 families and some derived eicosanoids from lipoxygenases, cyclooxygenases, and cytochrome P-450, seem to control the activity of transcription factor families involved in cancer cell proliferation or cell death. (springer.com)
- The 5-, 12- and 15-HETE are the main forms but others have been detected (8-, 9-, 11-, 15-, 17-, 18-, 19- and 20-HETE, the 16- to 20-HETE being formed via cytochrome P450). (gerli.com)
Metabolites1
- Furthermore, di-GA inhibited the generation of lymphendothelial gaps by cancer cell spheroid-secreted lipoxygenase metabolites. (nature.com)
Cyclooxygenases1
- The cyclooxygenases (COX-1 and 2) and lipoxygenase (5-LOX) assays were used for in vitro evaluations of compounds. (technoplus.ro)
Endothelial2
- Finally, activation of the immunoreceptor tyrosine-based inhibitory motif (ITIM)- containing platelet endothelial cell adhesion molecule (PECAM-1) inhibited p12-LOX product generation. (reading.ac.uk)
- Atherosclerosis starts with dysfunctional changes in the endothelium induced by disturbed shear stress which can lead to endothelial and platelet activation, adhesion of monocytes on the activated endothelium, and differentiation into proinflammatory macrophages, which increase the uptake of oxidized LDL (oxLDL) and turn into foam cells, exacerbating the inflammatory signalling. (hindawi.com)
Inhibitor1
- Licofelone is a cyclooxygenase (COX) and 5-lipoxygenase (5-LOX) inhibitor, which its effectiveness to treat osteoarthritis has been approved. (j-epilepsy.org)
Enzymes2
- Arachidonate 5-lipoxygenase is a member of the lipoxygenase family of enzymes. (wikipedia.org)
- The plant C18 fatty acids, linoleic acid and linolenic acid, can be converted by the enzymes a -dioxygenase, 9- or 13-lipoxygenase, giving rise to a multitude of oxylipins. (gerli.com)
Phospholipase2
- Mechanistically, 12(S)-HETE is produced through the concerted activity of secreted phospholipase A2 IIA (sPLA2-IIA), present in inflammatory fluids, and platelet-type 12-lipoxygenase (12-LO), expressed by platelet MPs. (evtrack.org)
- Protease-activated receptor signaling in platelets activates cytosolic phospholipase A2a differently for cyclooxygenase-1 and 12-lipoxygenase catalysis. (jefferson.edu)
Coronary2
- Platelet ACKR3/CXCR7 surface expression is increased and impacts prognosis in individuals with coronary artery disease (CAD), who have an atherothrombotic platelet lipidome. (physiciansweekly.com)
- Ex vivo, a pharmacological CXCR7 agonist (VUF11207) dramatically decreased prothrombotic platelet reactivity in blood from patients with the acute coronary syndrome. (physiciansweekly.com)
Mammalian1
- Part I: Enzymology, Molecular Biology, and Biological Functions of Mammalian Lipoxygenases. (edu.au)
Acute1
- These results also indicate that intravenously administered tocotrienols were significantly better than tocopherols in inhibiting cyclic flow reductions (CFRs), a measure of the acute platelet-mediated thrombus formation. (biomedcentral.com)
Pathways2
- Human platelets that contain a 12-LOX isoform (p12-LOX) were used to define pathways that activate H(P)ETE synthesis in the vasculature. (reading.ac.uk)
- Part III: Products of the Lipoxygenase Pathways and their Receptors. (edu.au)
Collagen1
- Collagen and collagen-related peptide (CRP) (1 to 10 g/mL) acutely induced platelet 12-H(P)ETE synthesis. (reading.ac.uk)
Inhibitors1
- Part II: Metabolic Regulation of Lipoxygenases: Lipoxygenase Inhibitors. (edu.au)
Phosphodiesterase1
- Moreover, phosphodiesterase 4D (PDE4D) [ 13 ], and 5-lipoxygenase gene activating protein (ALOX5AP) [ 14 ], were shown to be significantly related with ischemic stroke due to progressive changes in the walls of blood vessels resulting in the increased atherosclerosis process. (biomedcentral.com)
Vivo1
- In vivo, treatment of a CXCR7 agonist decreased thrombotic activities and thrombo inflammatory platelet leukocyte interactions following myocardial infarction and arterial damage. (physiciansweekly.com)
Docosahexaenoic acid1
- The aim of this open-label study was to determine if consumption of a high potency fish oil supplement could improve blood levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and impact SF-12 mental and physical health scores in healthy adults. (biomedcentral.com)
Promotes1
- Using a combination of genetically modified mice, we show that the coordinated action of sPLA2-IIA and 12-LO promotes inflammatory arthritis. (evtrack.org)
Clinical1
- Some adult patients required treatment for up to 12 weeks in clinical trials. (nih.gov)
Myocardial1
- Tocotrienols, given intravenously, could potentially prevent pathological platelet thrombus formation and thus provide a therapeutic benefit in conditions such as stroke and myocardial infarction. (biomedcentral.com)
Protein2
- The ALOX5 gene, which occupies 71.9 kilobase pairs (kb) on chromosome 10 (all other human lipoxygenases are clustered together on chromosome 17), is composed of 14 exons divided by 13 introns encoding the mature 78 kilodalton (kD) ALOX5 protein consisting of 673 amino acids. (wikipedia.org)
- In response to the presence of certain activating substances, including oxidized LDL, monocyte chemoattractant protein 1 (MCP-1), interleukin (IL)-8, and platelet-derived growth factor (PDGF), leukocytes migrate into the wall of the artery (see the image below). (medscape.com)
Oxylipins1
- These regio-specific enzymatic reactions between oxygen and polyunsaturated fatty acids formed new compounds, named oxylipins, which are most frequently with the S-configuration (5-HpETE, 12-HpETE or 15-HpETE). (gerli.com)
Gene1
- Arachidonate 5-lipoxygenase, also known as ALOX5, 5-lipoxygenase, 5-LOX, or 5-LO, is a non-heme iron-containing enzyme (EC 1.13.11.34) that in humans is encoded by the ALOX5 gene. (wikipedia.org)
Fatty acids1
- Some polyhydroxy fatty acids are also known, which are most frequently produced by lipoxygenase activities, as for several mono-hydroxylated fatty acids. (gerli.com)
Lung1
- 10 Anticancer effects of this agent in lung, 11 colon and small intestine 12 have been proved. (j-epilepsy.org)
Processes1
- Microglia are efficient phagocytes of synaptic material and apoptotic cells, which are key processes in the developing brain 12 . (nature.com)
Molecular1
- The empirical formula is C 15 H 12 ClNO 2 and the molecular weight 273.72. (drugs.com)
Adults1
- Limitations of Use: Safety and effectiveness of balsalazide beyond 8 weeks in children (ages 5 to 17 years) and 12 weeks in adults have not been established. (nih.gov)
Mechanisms1
- Whereas EVs are accepted as an important means of intercellular communication, the mechanisms underlying platelet MP internalization in recipient cells are poorly understood. (evtrack.org)
Erythrocytes1
- Platelets, T cells, and erythrocytes are ALOX5-negative. (wikipedia.org)
Blood3
- Platelets are anucleated blood elements highly potent at generating extracellular vesicles (EVs) cal ( show more. (evtrack.org)
- monitor complete blood cell counts and platelet counts. (nih.gov)
- Our patients depend on blood and platelet donations. (mdanderson.org)
Potent1
- Furthermore, 12-HETE may be transformed into more potent molecules, e.g. 10,11-dihydro-12-HETE. (gerli.com)
Cognitive1
- Our results show that maternal dietary n-3 PUFA deficiency increases microglia-mediated phagocytosis of synaptic elements in the rodent developing hippocampus, partly through the activation of 12/15-lipoxygenase (LOX)/12-HETE signaling, altering neuronal morphology and affecting cognitive performance of the offspring. (nature.com)
Effect1
- For a study, researchers sought to verify the role of ACKR3/CXCR7 in modulating thromboinflammatory response via its effect on the platelet lipidome. (physiciansweekly.com)
Research1
- Circulation Research, 94 (12). (reading.ac.uk)
Plasma7
- Next, pharmacokinetic studies were carried out and tocol levels in canine plasma and platelets were measured. (biomedcentral.com)
- As expected, α-Tocopherol treatment increased levels of total tocopherols in post- vs pre-treatment specimens (57 vs 18 μg/mL in plasma, and 42 vs 10 μg/mL in platelets). (biomedcentral.com)
- However, treatment with α-tocopherol resulted in slightly decreased levels of tocotrienols in post- vs pre-treatment samples (1.4 vs 2.9 μg/mL in plasma and 2.3 vs 2.8 μg/mL in platelets). (biomedcentral.com)
- tocotrienols, 2.8 vs 0.9 μg/mL in plasma and 1.28 vs 1.02 μg/mL in platelets). (biomedcentral.com)
- tocotrienols, 8.6 vs 1.7 μg/mL in plasma and 3.8 vs 3.9 μg/mL in platelets). (biomedcentral.com)
- α-Tocotrienols treatment induced increases in α-tocopherol levels of 4-fold and 6-fold in plasma and platelets, respectively. (biomedcentral.com)
- Interestingly, tocotrienols (TRF) treatment induced a less pronounced increase in the levels of tocotrienols in plasma and platelets, suggesting that intravenously administered tocotrienols may be converted to tocopherols. (biomedcentral.com)