High- and low-resolution structures of the MORN1 protein from Trypanosoma brucei and homologous proteins from the parasitesToxoplasma gondii and Plasmodium falciparum were obtained using a combination of macromolecular crystallography, small-angle X-ray scattering, and electron microscopy. (biorxiv.org)
Every year up to 500 million people around the world are infected with Plasmodium falciparum - the parasite that causes malaria - and around 1/2 million people will die from the disease. (gulbenkian.pt)
Toxoplasma and Plasmodium falciparum are two distantly related eukaryotic, single cell parasites of humans and animals that live and replicate in cells of their eukaryotic host. (gulbenkian.pt)
Here the lipid profiles of red blood cells infected with the five different sexual stages of Plasmodium falciparum were analysed by mass spectrometry and compared to those from uninfected and asexual trophozoite infected erythrocytes. (biomedcentral.com)
The prolonged duration of gametocyte maturation is a unique feature of only a few Plasmodium species infecting higher primates ( P. falciparum and P. reichenowi ) [ 1 , 2 ]. (biomedcentral.com)
Infection with P. falciparum causes by far the highest morbidity of all human Plasmodium species. (biomedcentral.com)
Pires I, Hung YF, Bergmann U, Molloy JE & Kursula I (2022) Analysis of Plasmodium falciparum myosin B ATPase activity and structure in complex with the calmodulin-like domain of its light chain MLC-B. J Biol Chem 298: 102634. (uib.no)
Asexual populations of the human malaria parasite, Plasmodium falciparum, use a two-step genomic strategy to acquire accurate, beneficial DNA amplifications. (rathodlab.org)
A genetically hard-wired metabolic transcriptome in Plasmodium falciparum fails to mount protective responses to lethal antifolates. (rathodlab.org)
The parasites we work with, Plasmodium falciparum and Toxoplasma gondii both evolved to excel at infecting and modifying host cells and a huge proportion of the proteins important for these processes are poorly characterized. (treecklab.org)
A multiplex bead-based assay simultaneously detected IgG to Anopheles albimanus salivary gland extract (SGE) and four Plasmodium falciparum antigens (CSP, LSA-1, PfAMA1, and PfMSP1) in 11,541 children enrolled at 350 schools across Haiti in 2016. (cdc.gov)
Parasites7
2017 ). The circulation of T.gondii parasites across the fetal placenta barrier results in miscarriage, preterm delivery, death in utero, neonatal growth retardation, hydrocephalus, cerebral calcification, neurological or ophthalmic disease in the new-born, during childhood or adolescence (Van der Colf et al. (researchsquare.com)
We therefore introduce the reader to CRISPR-based screens in the related apicomplexan Toxoplasma gondii and discuss how these approaches could be adapted to develop CRISPR/Cas9 based genome-scale genetic screens in malariaparasites. (portlandpress.com)
Salamun J, Kallio JP, Daher W, Soldati-Favre D & Kursula I (2014) Structure of Toxoplasma gondii coronin - an actin-binding protein that relocalizes to the posterior pole of invasive parasites and contributes to invasion and egress. (uib.no)
But the organism has added importance because it's a close relative of several other parasites, including Plasmodium , which causes malaria. (dartmouth.edu)
We report here the identification of seven putative aspartic proteases, TgASP1 to TgASP7, in the apicomplexan parasite Toxoplasma gondii. (unige.ch)
Infection13
Toxoplasma gondii ( T. gondii ) infection during pregnancy is associated with a variety of complications to the mother and baby. (researchsquare.com)
In Tanzania, there is a paucity of data on exposure T. gondii infection among pregnant women and the associated risk factors. (researchsquare.com)
The seroprevalence of T. gondii infection ranges from 4-78% among pregnant women in endemic countries (Mwambe et al. (researchsquare.com)
Infection with another parasite, Toxoplasma gondii , is less dangerous but more widespread, infecting up to a third of the world's population. (gulbenkian.pt)
At the same time, the weakened strain prompted an immune response that protected the mice against infection by virulent strains of Toxoplasma . (dartmouth.edu)
The way of anyone Plasmodium parasite susceptibility and infection around is animals causes infects to form host tested protection range in. (hotelvikasinn.com)
What are the common and specific tools different Toxoplasma strains use to succeed in infection? (treecklab.org)
I will be using our arrayed CRISPR library with both in vitro and in vivo selection methods to identify which of those proteins is important for Toxoplasma 's success to establish an infection. (treecklab.org)
Francesca completed her PhD in the Seeber lab at the Robert Koch Institute in Berlin, working on the resistance mechanisms of wild rodents to Toxoplasma infection. (treecklab.org)
We have had similar lack of success with attempts at expression in the related protozoan parasite Toxoplasma gondii. (bio.net)
Current Research and Scholarly Interests We are intereseted in the interaction between the protozoan parasite Toxoplasma gondii and its mammalian host. (stanford.edu)
B y knocking out a key gene in the -parasite Toxoplasma gondii , DMS researchers hope to strike a blow for global health. (dartmouth.edu)
Tachyzoites1
TgASP1 is expressed in tachyzoites, the rapidly dividing asexual stage of T.gondii. (unige.ch)
Eight cases of locally acquired, mosquito-transmitted (i.e., autochthonous) Plasmodium vivaxmalaria, which has not been reported in the United States since 2003, were reported to CDC from state health departments in Florida and Texas during May 18-July 17, 2023. (cdc.gov)
Seroprevalence2
Therefore, this study investigated the seroprevalence of T. gondii and associated factors among pregnant women attending antenatal care in Ilala Municipality, Dar es Salaam. (researchsquare.com)
Seroprevalence of antibody to TgGRA7 antigen of Toxoplasma gondii in livestock animals from Western Java, Indonesia. (obihiro.ac.jp)
Proteins1
Toxoplasma secretes a large number of proteins that mediate adhesion and invasion, establishment and defence of the parasitophorous vacuole, and influence various host cell pathways. (treecklab.org)
Babesia2
Additional members of the phylum Apicomplexa, important to global human and animal health include the organisms in the genus Plasmodium as well as T . parva and T . annulata , and Babesia bovis causes of malaria, bovine theileriosis and babesiosis, respectively. (biomedcentral.com)
The phylum Apicomplexa comprises human pathogens such as Plasmodium but is also an under-explored hotspot of evolutionary diversity central to understanding the origins of parasitism and non-photosynthetic plastids. (elifesciences.org)
Asexual1
Moreover, since more than half of Plasmodium genes are required for normal asexual blood-stage reproduction, and cannot be targeted using knockout methods, we discuss how CRISPR/Cas9 could be used to scale up conditional gene knockdown approaches to systematically assign function to essential genes. (portlandpress.com)
Encephalitis2
Designated an orphan drug by FDA for primary prophylaxis in HIV-infected individuals at high risk for developing T. gondii encephalitis and for treatment and suppression of T. gondii encephalitis. (drugs.com)
They generally include Pneumocystis jiroveci pneumonia (PCP), toxoplasmaencephalitis, cryptococcal meningitis, cytomegalovirus (CMV) retinitis, tuberculosis (TB), disseminated Mycobacterium avium complex (MAC), or pneumococcal respiratory disease. (medscape.com)
Feces1
Acquisition of T.gondii is through the ingestion of tissue cysts in meat, ingestion of food, water, or soil contaminated with sporulated oocysts, and directly from the cat feces (Murebwayire et al. (researchsquare.com)
Functional1
Mueller C, Samoo A, Hammoudi PM, Klages N, Kallio JP, Kursula I & Soldati-Favre D (2016) Structural and functional dissection of Toxoplasma gondii armadillo repeats only protein. (uib.no)
Species1
IgG serology can be utilized to estimate exposure to Anopheline malaria vectors and the Plasmodium species they transmit. (cdc.gov)
Protozoa1
PROBLEM/CONDITION: Malaria in humans is caused by intraerythrocytic protozoa of the genus Plasmodium. (cdc.gov)
Actin1
Kumpula EP, Lopez AJ, Tajedin L, Han H & Kursula I (2019) Atomic view into Plasmodium actin polymerization, ATP hydrolysis, and fragmentation. (uib.no)
Strains1
With the NHEJ problem solved, creating new strains of Toxoplasma for use against these diseases will be much easier. (dartmouth.edu)
A five mL of blood sample was collected from each of the recruited pregnant women, processed to obtain serum, and tested for the presence of IgG and IgM anti- T. gondii specific antibodies. (researchsquare.com)
Human1
Consumption of sheep meat presents a risk of human contamination by Toxoplasma gondii. (indexindex.com)
Protein1
Then they tried it with another protein, KU-80, and found that the resulting strain of Toxoplasma was much less likely to exhibit NHEJ. (dartmouth.edu)
Infections2
The majority of healthy individuals with T. gondii infections are asymptomatic. (researchsquare.com)
Routinely maternal screening through serological tests to monitor acute and latent T. gondii infections during pregnancy reduces the possibility of fetal infections and substantial damages. (researchsquare.com)
20021
In 2002, they created an attenuated, or weakened, strain of Toxoplasma that showed great promise as a vaccine. (dartmouth.edu)
Meat1
A nationwide study was conducted in France to evaluate the prevalence of Toxoplasma in fresh ovine meat. (indexindex.com)
Host1
He explains that the immune response prompted by Toxoplasma is just what's needed to combat a host of diseases, including malaria, tuberculosis, HIV, and cancer. (dartmouth.edu)
Screens1
In recent years technical breakthroughs have made forward and reverse genome-scale screens in Plasmodium possible. (portlandpress.com)
Specific2
When Toxoplasma encounters foreign DNA, it randomly inserts that DNA into its genome in a process called nonhomologous end-joining (NHEJ), making it hard for scientists to manipulate the organism's DNA and target specific genes. (dartmouth.edu)
They thought it might be possible to identify-and knock out-a specific gene responsible for allowing Toxoplasma to exhibit this behavior. (dartmouth.edu)
Study1
David Bzik, Ph.D., a professor of microbiology and immunology, and Barbara Fox, a research associate in Bzik's lab, study Toxoplasma as a model organism that may help fight malaria and other diseases. (dartmouth.edu)