• Plasmids were first clearly implicated in this conjugative process in the most studied strain, Streptomyces coelicolor A3(2), when certain derivatives of the wild-type isolate were found to differ in their "fertility" properties-that is, in the frequency with which they generated chromosomal recombinants when mated with various other derivatives-and this ability was inherited "infectiously" (2, 28, 72). (springer.com)
  • Bibb, M. J., and Hopwood, D. A., 1981, Genetic studies of the fertility plasmid SCP2 and its SCP2' variants in Streptomyces coelicolor A3(2), J. Gen. Microbiol . (springer.com)
  • Basically, I need a plasmid that has two identical (and ideally inducible) promoters just upstream of the MCS on each strand. (protocol-online.org)
  • The global spread of MDR plasmids has been enhanced by selective pressure from antibiotic usage in human and veterinary medicine. (wikipedia.org)
  • I used sucrose gradients to isolate my plasmid preps - got good enough DNA to produce good restriction patterns, clone specific fragments etc. (bio.net)
  • In such procedures, a plasmid is cut at a specific site (or sites) using enzymes called restriction endonucleases. (britannica.com)
  • Maybe you run into problems with one plasmid curing the cell of the other if you have plasmids based on complete 2u sequence. (bio.net)
  • Ian, I've had much luck sith a 11.5 Kb low copy (pSC101) plasmid. (bio.net)
  • So long as you have a selection pressure for both plasmids, you should be alright - though the relative copy numbers may not be stable. (bio.net)
  • In addition, the new anion-exchange resin contained in HiSpeed Tips has an increased capacity, allowing yields of up to 200 g high-copy plasmid to be obtained from HiSpeed Midi Tips, and up to 750 g high-copy plasmid to be obtained from HiSpeed Maxi Tips. (bio-medicine.org)
  • The number may even reach thousands for certain artificial plasmids (such as the pUC series of plasmids) selected for high copy number. (newworldencyclopedia.org)
  • Such single-copy plasmids have systems that are oriented toward actively distributing a copy to both daughter cells. (newworldencyclopedia.org)
  • Daughter cells that retain a copy of the plasmid survive, while a daughter cell that fails to inherit the plasmid dies or suffers a reduced growth-rate because of the lingering poison from the parent cell. (newworldencyclopedia.org)
  • Hello all, I would like to transcribe both the sense and antisense strands of a clone in some type of high-copy number plasmid. (protocol-online.org)
  • We store and distribute high-quality plasmids from your colleagues. (addgene.org)
  • We archive and distribute high quality plasmids from your colleagues. (addgene.org)
  • The size of plasmids varies from 1 to over 400 kilobase pairs (kbp). (newworldencyclopedia.org)
  • These plasmids are interesting because of their size, they range between 100 and 1800 Kb, and in some strains they represent about 50% of the bacterial genome. (elsevier.com)
  • The effect of killing plasmid-free daughter cells appears, at the population level, to ''addict'' the host to the plasmid. (newworldencyclopedia.org)
  • When integrated into the host cell's genome, pTK-Hyg does not cause unwanted activation of pTRE- and pBI-derived plasmids because the HSV TK promoter lacks an enhancer element. (clontech.com)