RP4-mediated transfer of mobilizable plasmids in intergeneric conjugation of Escherichia coli donors with Corynebacterium glutamicum ATCC 13032 is severely affected by a restriction system in the recipient that can be inactivated by a variety of exogenous stress factors. (uni-bielefeld.de)
Nitrogen-fixing cyanobacterium Anab1
Disruption of the uptake hydrogenase gene, but not of the bidirectional hydrogenase gene, leads to enhanced photobiological hydrogen production by the nitrogen-fixing cyanobacterium Anabaena sp. (wikigenes.org)
Conjugation experiments of different mobilizable plasmids, helped by either FI-susceptible or FI-resistant transfer systems, demonstrated that the conjugative component affected by both PifC and Osa was the type IV conjugative coupling protein. (frontiersin.org)
Bacteria3
Bacteria display a variety of mechanisms to control plasmid conjugation. (frontiersin.org)
For example, all conjugative plasmids code for exclusion systems that act in recipient bacteria to prevent competition between identical plasmid backbones, block uneconomical excess of conjugative transfer, and protect recipients from lethal zygosis ( Garcillan-Barcia and de la Cruz, 2008 ). (frontiersin.org)
FI systems are best known as regulators of plasmid transfer in donor bacteria. (frontiersin.org)
By using this procedure, clones of the restriction-deficient mutant strain C. glutamicum RM3 harboring a plasmid library of the wild-type chromosome were checked for their restriction properties. (uni-bielefeld.de)
PCC71201
CpcS from Anabaena PCC7120 is a relatively simple system, it acts as a monomer, and does not require any cofactors. (uni-muenchen.de)
Conjugation7
Among them, fertility inhibition (FI) systems prevent conjugation of co-resident plasmids within donor cells. (frontiersin.org)
In this work, inhibition of conjugation of broad host range IncW plasmids was analyzed in the presence of a set of co-resident plasmids. (frontiersin.org)
Strong FI systems against plasmid R388 conjugation were found in IncF/MOB F12 as well as in IncI/MOB P12 plasmids, represented by plasmids F and R64, respectively. (frontiersin.org)
It was also discovered that the R388 gene osa , which affects T-DNA transfer, also prevented conjugation of IncP-1/MOB P11 plasmids represented by plasmids RP4 and R751. (frontiersin.org)
Since the relevant products act in trans , the FinOP system collaterally inhibits conjugation of related plasmids ( Frost and Koraimann, 2010 ). (frontiersin.org)
Beyond FinOP, there exist "unusual" FI systems that affect conjugation of unrelated co-resident plasmids. (frontiersin.org)
This implies that FI are rapidly-moving accessory genes, possibly acting on evolutionary fights between plasmids for the colonization of specific hosts. (frontiersin.org)
Among them, different plasmids (IncI1, IncFI, CloDF13-like, and IncX) inhibit the transfer of IncF plasmids, although the responsible genes were not characterized ( Gasson and Willetts, 1975 ). (frontiersin.org)
Understanding the interaction network between transmissible plasmids is essential to know how the main carriers of antibiotic resistance genes disseminate in the environment. (frontiersin.org)
After cloning of apophycobiliprotein genes, phycobilin biosynthesis genes and (putative) lyase genes from several cyanobacteria, various phycobiliproteins could be biosynthesized in the heterologous E. coli system using dual plasmids containing the respective genes. (uni-muenchen.de)
Analysis of the mechanisms of inhibition between conjugative plasmids could provide new alternatives to fight antibiotic resistance dissemination. (frontiersin.org)
A complemented clone with a restriction-positive phenotype was isolated and found to contain a plasmid with a 7-kb insertion originating from the wild-type chromosome. (uni-bielefeld.de)
Transfer3
In both cases, the responsible gene was pifC , known also to be involved in FI of IncP plasmids and Agrobacterium T-DNA transfer to plant cells. (frontiersin.org)
Mechanisms to control conjugative transfer are also present in plasmids themselves. (frontiersin.org)
In this study a rapid test procedure based on intergeneric conjugal plasmid transfer that permitted the distinction between restriction-negative and restriction-positive C. glutamicum clones was developed. (uni-bielefeld.de)
Functions2
PlmA, a new member of the GntR family, has plasmid maintenance functions in Anabaena sp. (wikipedia.org)
Recently Zhao and co-workers discovered that a CpeS-like protein functions as a phycocyanobilin-cysteine-beta84 lyase in Anabaena sp. (biomedcentral.com)
System1
This is the case of the FinOP system of IncF plasmids ( Frost and Koraimann, 2010 ). (frontiersin.org)
Analysis1
In addition, in silico analysis of FI proteins suggests that they represent recent acquisitions of conjugative plasmids, i.e., are not shared by members of the same plasmid species. (frontiersin.org)
Variabilis1
We evaluated the performance of LTC2 ( Ls GAS) as well as nine other identified or putative germacrene A synthases from different sources for the production of germacrene A. Av GAS, a synthase of Anabaena variabilis , was found to be the most efficient in germacrene A production in yeast. (biomedcentral.com)
Cloning vectors1
Addgene: Paired cloning vectors for complementation of mutations in the cyanobacterium Anabaena sp. (addgene.org)
Nostoc1
Shuttle vector suitable for transfer to, and replication in, E. coli and Anabaena (Nostoc) sp. (addgene.org)
Escherichia1
RP4-mediated transfer of mobilizable plasmids in intergeneric conjugation of Escherichia coli donors with Corynebacterium glutamicum ATCC 13032 is severely affected by a restriction system in the recipient that can be inactivated by a variety of exogenous stress factors. (uni-bielefeld.de)
Protein3
The small Ca2+-binding protein CSE links Ca2+ signalling with nitrogen metabolism and filament integrity in Anabaena sp. (biomedcentral.com)
Landmark discovery on demonstration of expression and modulation of cholera toxin biosynthesis by plasmids (Nature, 1978) and adhesive protein of V. cholerae for binding to intestinal mucosa and the concept of the subunit vaccine in experimental cholera. (muhaz.org)
A complemented clone with a restriction-positive phenotype was isolated and found to contain a plasmid with a 7-kb insertion originating from the wild-type chromosome. (uni-bielefeld.de)