Plasma phospholipid antibodies. Medical search. Frequent questions

PE Annexin V is a sensitive probe for identifying apoptotic cells, binding to negatively charged phospholipid surfaces (Kd of ~5 x 10e-2) with a higher affinity for phosphatidylserine (PS) than most other phospholipids. (bdbiosciences.com)
b2GPI, also called apolipoprotein H, is an antiphospholipid protein cofactor with natural anticoagulant properties and an affinity for negatively-charged phospholipids. (diapharma.com)
Apolipoprotein H (APOH) attaches to numerous kinds of negatively charged substances for instance heparin, phospholipids, and dextran sulfate. (assaygenie.com)
APOH: Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. (assaygenie.com)
It is a blood plasma protein that binds to negatively charged phospholipids, in particular cardiolipin. (lab24.pl)
Primary APAS as well as systemic lupus erythematosus (SLE) are characterized by the appearance of autoantibodies to negatively charged phospholipids (1). (eaglebio.com)
The positively charged fifth domain of ß2 GP-I interacts with negatively charged phospholipids or activated polystyrol surfaces of ELISA wells. (eaglebio.com)
In addition, when platelets are activated, negatively charged phospholipids move from the inner to the outer leaflet of the membrane bilayer. (medscape.com)

Autoimmune antiphospholipid antibodies prolonged the dilute Russell viper venom time of normal plasma, but had no effect on beta 2GPI-depleted plasma. (jci.org)
In the clinical laboratory, antiphospholipid syndrome is associated with anticardiolipin antibodies, beta-2 glycoprotein 1 antibodies, and the lupus anticoagulant (or lupus inhibitor) which causes prolongation of phospholipid-dependent coagulation assays such as the PTT or dilute Russell viper venom time (dRVVT). (msdmanuals.com)

Bavituximab is an antibody that binds to complexes of the phosphatidylserine-binding plasma protein β2-glycoprotein 1 and anionic phospholipids. (wikipedia.org)
In vitro beta 2GPI binds to anionic phospholipids and inhibits the prothrombinase activity of procoagulant membranes. (jci.org)
After adding enzyme conjugate, it binds to the antibody-antigen complex. (rapidtest.com)
Beta-2 glycoprotein 1 in plasma binds to phospholipid-rich surfaces. (msdmanuals.com)
We previously cloned the IgM natural antibody E06, which binds to the phosphocholine headgroup of OxPL, and blocks the uptake of oxidized low-density lipoprotein (OxLDL) by macrophages and inhibits the proinflammatory properties of OxPL. (fondationleducq.org)

Lupus anticoagulant activity of autoimmune antiphospholipid antibodies is dependent upon beta 2-glycoprotein I. (jci.org)
It has been reported that antiphospholipid autoantibodies do not recognize phospholipid alone, but rather the plasma protein beta 2-glycoprotein I (beta 2GPI), or a beta 2GPI-phospholipid complex. (jci.org)
Anticardiolipin and Anti-Beta-2 Glycoprotein 1 Antibodies. (dobratechnologia.pl)
anticardiolipin (aCL), which has three subcomponents: lgG, lgA, lgM anti-Beta-2-glycoprotein-I (aβ 2 GPI), which has the same three subcomponents LAC The LAC is the most important of these antibodies because it confers the greatest risk for developing clinical complications. (dobratechnologia.pl)
that is characterized by arterial, venous, or microvascular thrombosis or recurrent pregnancy loss caused by antibodies directed against one or more phospholipid-bound proteins (eg, beta-2 glycoprotein 1, prothrombin, annexin A5). (msdmanuals.com)
Antibodies to beta-2 glycoprotein 1 upregulate cellular adhesion proteins, such as E selectin, and procoagulant proteins, such as tissue factor. (msdmanuals.com)
In addition, antibodies to beta-2 glycoprotein 1 downregulate expression of tissue factor pathway inhibitor, an endogenous anticoagulant protein. (msdmanuals.com)
What is the role of Beta 2 Glycoprotein 1 (B2GP1) in anti-phospholipid antibody binding? (diapharma.com)
Objectives Evaluating the effect of repeated FTC on anticardiolipin (aCL) IgM/IgG and anti\beta\2 glycoprotein 1 (a2GPI) IgM/IgG antibody GW 542573X titer. (nanoker-society.org)
The three main cornerstone antibodies are anticardiolipin (aCL) IgM/IgG, anti\beta\2 glycoprotein 1 antibodies (a2GPI) IgM/IgG, and lupus anticoagulant (LAC). (nanoker-society.org)
a2GPI, anti\beta 2 glycoprotein I. Citrated plasma was freezing after double centrifugation.3 Samples were collected between 2012 and 2017. (nanoker-society.org)
Antibodies to beta-2-glycoprotein (IgG) are one of the markers in the diagnosis of antiphospholipid syndrome (APS). (lab24.pl)
Beta-2-glycoprotein is a cofactor necessary for the interaction of antiphospholipid antibodies with phospholipids. (lab24.pl)
This disorder is characterized by persistently elevated concentrations of antibodies directed against membrane anionic phospholipids (eg, anticardiolipin antibody, antiphosphatidylserine) or their associated plasma proteins, predominantly beta-2 glycoprotein I (apolipoprotein H). (nursingclassessays.org)

The modeling platform developed here uncovers insights into lipid participation in antibodies' recognition of membrane proteins and highlights antibody features to prioritize in vaccine design. (elifesciences.org)
Antibodies can target epitopes on integral membrane proteins very near to the lipid bilayer surface, even those partially embedded within the headgroup region. (elifesciences.org)
Annexin I belongs to a family of Ca (2+) -dependent phospholipid binding proteins that are preferentially located on the cytosolic face of the plasma membrane. (thermofisher.com)
Antiphospholipid syndrome is an autoimmune disorder characterized by venous and arterial thrombosis or pregnancy complications (eg, recurrent miscarriage) and persistent autoantibodies to phospholipid-bound proteins. (msdmanuals.com)
Annexins are a multigene family of Ca++-regulated phospholipid-binding proteins. (rndsystems.com)
The name annexin derives from the fact that initial studies suggested that acidic membrane phospholipids and Ca++ annexed, or recruited, special cytosolic proteins into a membrane-interacting ternary complex. (rndsystems.com)
After their removal of unbound serum proteins by washing, antibodies specific for human IgG, IgM, or IgA labeled with HRP are added forming complexes with the CL bound antibodies. (diapharma.com)
Antiphospholipid (APL) antibodies are group of antibodies directed against epitopes on plasma proteins that are uncovered by binding of these proteins to anionic phospholipids on plasma membranes. (medscape.com)
It is an autoimmune disease in which antiphospholipid antibodies (anticardiolipin antibodies and lupus coagulant) interact with proteins that bind to anionic phospholipids on plasma membranes. (lab24.pl)
Antibodies are proteins produced by the immune system to help fight off infection and illness. (endinglines.com)
In APS, the immune system produces abnormal antibodies that rather than attacking bacteria and viruses, mistakenly attack proteins found on the outside of cells in the blood and blood vessels. (endinglines.com)
antibodies: proteins synthesized by plasma cells that are released into the circulation to the antigen site and destroy the microorganisms by chemically reacting with them. (studygate.com)
After washing to remove unbound plasma proteins, enzyme conjugated anti-human immunoglobulin specific for IgA is added. (diapharma.com)

Patients who have normal initial test results, along with symptoms or signs of bleeding and a positive family history, should be tested for von Willebrand disease by measuring plasma von Willebrand factor (VWF) antigen, ristocetin cofactor activity (an indirect test of VWF function), VWF multimer pattern, and factor VIII levels. (merckmanuals.com)
Recent studies have shown that a 50kD serum cofactor is required for anticardiolipin antibodies, to bind to cardiolipin which has been coated onto plastic plates. (rapidtest.com)
This includes antibodies specific for the cardiolipin molecule itself, and antibodies that are directed against either a cofactor molecule such as b2GPI, or a special binding site created by the interaction of a cofactor with CL. (diapharma.com)
Antibodies directed against CL may be associated with infectious disease, or may be specific for a different cofactor, such as prothrombin. (diapharma.com)
Most autoimmune anti-phospholipid antibodies require the serum cofactor b2GPI for optimal binding. (diapharma.com)
APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome, but it does not seem to be required for the reactivity of antiphospholipid autoantibodies associated with infections. (assaygenie.com)
In the presence of cofactor factor Va, phospholipid, and Ca 2+ , activated factor X cleaves two peptide bonds in prothrombin to form thrombin. (greenmoab.com)
Unlike phospholipid antibodies which occur in some patients having infectious disease, phospholipid antibodies of autoimmune disease patients seem to recognize the relevant phospholipids in association with a plasma protein cofactor. (eaglebio.com)

Phosphatidylserine is the most abundant anionic phospholipid in the cell's plasma membrane, and is usually positioned internally. (wikipedia.org)
A potent class of HIV-1 broadly neutralizing antibodies (bnAbs) targets the envelope glycoprotein's membrane proximal exposed region (MPER) through a proposed mechanism where hypervariable loops embed into lipid bilayers and engage headgroup moieties alongside the epitope. (elifesciences.org)
All-atom simulations of 4E10, PGZL1, 10E8 and LN01 docked onto HIV-like membranes consistently form phospholipid complexes at key complementarity-determining region loop sites, solidifying that stable and specific lipid interactions anchor bnAbs to membrane surfaces. (elifesciences.org)
This valuable study reports multi-scale molecular dynamics simulations to investigate a class of highly potent antibodies that simultaneously engage with the HIV-1 Envelope trimer and the viral membrane. (elifesciences.org)
The work provides insights into how broadly neutralizing antibodies associate with lipids proximal to membrane-associated epitopes to drive neutralization. (elifesciences.org)
A better understanding of how antibodies develop membrane affinity and target membrane-proximal epitopes would be impactful for antibody therapeutics, auto-immunity, and vaccine development 10 - 12 . (elifesciences.org)
We sought to address this phenomenon for broadly neutralizing antibodies (bnAbs) 4E10, PGZL1, 10E8, and LN01 of unique lineages which all target the semi-concealed membrane-proximal epitope region (MPER) 13 - 16 of the HIV-1 envelope glycoprotein (Env). (elifesciences.org)
Neuronal α-synuclein is concentrated in presynaptic nerve terminals, interacts with plasma membrane phospholipids, and is also present in nuclei and mitochondria. (biolegend.com)
Changes in plasma membrane phospholipids inhibit antibody-mediated lysis. (bvsalud.org)
They serve as membrane-membrane, or membrane-cytoskeleton linkers, and regulate the formation of lipid domains in the plasma membrane. (rndsystems.com)
Annexins possess a series of unique 70 amino acid annexin-repeat modules that form a curved disk and allow for the creation of an intercessory complex between plasma membrane and cytofilaments. (rndsystems.com)
membrane phospholipids, and other anionic phospholipid-containing moieties. (bvsalud.org)
Mouse anti Human CD142 antibody, clone TF9-10H10 recognizes human CD142, also known as Tissue Factor, is the membrane receptor for coagulation factors VII and VIIa and is the cell surface initiator of coagulation. (bio-rad-antibodies.com)
Phagocytosis requires the internalization of a significant fraction of the plasma membrane, which results in the intracellular deposition of large particles [ 3354 ]. (nanomedicine.com)
Classification criteria for the diagnosis of antiphospholipid syndrome include: Lupus anticoagulant (LA), cardiolipin antibodies (ACL IgG, ACL IgM), and antibodies against β2-glycoprotein I (anti-β2GP-I), which in vitro have antigen-ic plasma protein-protein epitopes associated with charged phospholipid-membrane com-plexes and their presence in vivo in serum has been associated with the occurrence of ar-terial and venous thrombosis and complications in pregnancy. (uniwa.gr)

Autoantibodies are antibodies to self components produced when immunologic tolerance is broken. (diapharma.com)
This interaction results in conformational changes of ß2 GP-I and the creation of new epitopes apparently recognized by autoimmune phospholipid autoantibodies. (eaglebio.com)

It has been shown conclusively that Beta2GP1 is indeed the antigen to which many anticardiolipin antibody patients are actually binding and furthermore showed that the phospholipid merely serves to link the Beta2GP1 to the solid phase. (rapidtest.com)
Anticardiolipin Antibody LabcorpThey are also found in up to 20% of young stroke patients. (dobratechnologia.pl)
Test Code: 161950 Specimen Type: Blood Description: Anticardiolipin Antibody IgA, IgG, IgM Blood Test (Labcorp) This test looks for 3 types of Cardiolipin Antibodies, IgA, IgM, and IgG. (dobratechnologia.pl)
[rx] These antibodies are often referred to by different terms, including anticardiolipin antibody, lupus anticoagulant, and antiphospholipid antibody. (endinglines.com)

The results of the assay are to be used as an aid in the diagnosis of certain autoimmune disease thrombotic disorders, anti-phospholipid syndrome, SLE or lupus-like disorders. (rapidtest.com)
The determination of IgM antibodies is a valuable indicator in the diagnosis of beginning autoimmune disease, whereas IgG and/or IgA antibodies will be found in progressive stages of manifested autoimmune disorders. (rapidtest.com)
Antiphospholipid syndrome is an autoimmune disease characterised by the presence of antiphospholipid antibodies, combined with a history of thrombosis (arterial and/or ve-nous) and thrombocytopenia. (uniwa.gr)

In the process of engaging the antigen, antibodies complementarity-determining regions (CDR) may need to navigate interactions with or concealment by lipid molecules. (elifesciences.org)
Following antigen retrieval using 88% formic acid for 20 minutes at room temperature, the tissues were incubated with 5 µg/mL of the primary antibody for 60 minutes at room temperature. (biolegend.com)
Autoimmune Disorders In autoimmune disorders, the immune system produces antibodies to an endogenous antigen (autoantigen). (msdmanuals.com)
Diluted serum samples, calibrator sera, and controls are incubated in cardiolipin coated microwells, allowing aCL antibodies present in the samples to react with the immobilized antigen. (diapharma.com)
When trimeric H3 HA was presented on liposomes, antigen delivery to macrophages was improved in vitro, and strong functional antibody responses were induced following intramuscular immunization of mice. (cdc.gov)
To demonstrate the flexibility and modular nature of the liposome system, 10 recombinant surface antigens representing distinct influenza virus strains were bound simultaneously to generate a highly multivalent protein particle that with 5 ng individual antigen dosing induced antibodies in mice that specifically recognized the constituent immunogens and conferred protection against heterologous H5N1 influenza virus challenge. (cdc.gov)

In the subjects of family population I, plasma levels of antibodies (IgG, IgM, IgA) against experimental epitopes (malondialdehyde-acetaldehyde-modified, copper-oxidized) of oxidized LDL (low-density lipoprotein) particles were measured. (oulu.fi)
While polyclonal antibodies are secreted by many different B cell clones and recognize multiple antigenic epitopes, monoclonals originate from a single B cell clone and are specific for just one epitope. (cellsignal.com)
The molecular composition and binding epitopes of the immunoglobulin G (IgG) antibodies that circulate in blood plasma following SARS-CoV-2 infection are unknown. (cdc.gov)

Rational Laboratory Diagnostics of Antiphospholipid Antibodies: Anti-Cardiolipin, Anti-β2-Glycoprotein I, Anti-Prothrombin and Anti-Annexin V Antibodies. (ncbcs.org)
The presence of anti-cardiolipin antibodies in SLE can be related to the development of thrombocytopenia, in gynaecology they are supposed to cause intrauterine death or recurrent abortion. (rapidtest.com)
Furthermore, anti-cardiolipin antibodies have been found in some nonthrombotic neurological disorders like cerebrovascular insufficiency, cerebral ischemia or chorea and in myocardial infarction. (rapidtest.com)
Anti-cardiolipin antibodies are by definition a major criterion for a diagnosis of APS. (dobratechnologia.pl)
What is the clinical significance when samples test positive for the anti-cardiolipin antibodies and are negative for anti-b2GPI? (diapharma.com)
APOH is also more specific than anti-cardiolipin antibodies and its presence correlates better with thrombotic risk. (assaygenie.com)
Hughes Syndrome/Antiphospholipid syndrome (APS) is a systemic autoimmune, hypercoagulable, thrombo inflammatory, and thrombosis and/or pregnancy complications syndrome caused by the persistent presence of antiphospholipid antibodies (APL) in plasma of patients with vascular thrombosis and/or pregnancy morbidity along with persistent anti-phospholipid antibodies (APLA), including lupus anticoagulant (LA), anti-β2-glycoprotein I (anti-β2GPI) and/or anti-cardiolipin (ACL) antibodies. (endinglines.com)
REAADS Anti-Cardiolipin (aCL) IgA is an ELISA for the determination of IgA anti-cardiolipin antibodies in human serum or plasma in individuals with systemic lupus erythematosus (SLE) and lupus-like disorders (anti-phospholipid syndrome). (diapharma.com)

Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. (abcam.com)
Traditional polyclonal and monoclonal antibodies are the product of normal B cell development and genetic recombination. (cellsignal.com)
In contrast, hybridoma-based systems for producing monoclonal antibodies are subject to genetic drift and instability, increasing the potential for lot-to-lot variability or loss of antibody expression. (cellsignal.com)

N.B. Antibody reactivity and working conditions may vary between species. (bio-rad-antibodies.com)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Phospholipid Transfer Protein (PLTP) in samples from Serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species. (elisareagents.com)

The presence of anti-phospholipid antibodies (aPL) have been outlined by our in-house ELISA strategies. (ncbcs.org)
Anti-annexin V antibodies have been measured with ELISA in 177 VOC and 81 steady-state SCA sufferers. (ncbcs.org)
Sera of 40 scleroderma sufferers and 15 wholesome controls have been examined for IgG and IgM anti-annexin V antibodies by ELISA and anticentromere antibodies by oblique immunofluorescence. (ncbcs.org)
The Diagnostic Automation Beta2GP1 IgA Enzyme-linked Immunosorbent Assay (ELISA) is intended for the detection and semiquantitative determination of IgA antibodies to Beta2GP1 in human sera or plasma. (rapidtest.com)
For example, a coagulation assay for the detection of LA and an ELISA for the detection of anti-phospholipid antibodies or cofactors like anti-CL or anti-b2GPI are recommended. (diapharma.com)
ELISA is the test used to detect these antibodies, IgM and IgG isotypes. (medscape.com)
Description: Quantitativesandwich ELISA kit for measuring Human phospholipid transfer protein(PLTP) in samples from serum, plasma, tissue homogenates. (elisareagents.com)
The Eagle Biosciences Anti-beta 2 GP-I ELISA Assay Kit is used for the quantitative determination of IgG or IgM antibodies to ß2 glycoprotein-I (beta2 GP-I) in human serum or plasma for the diagnosis of anti-phospholipid antibody syndrome (APAS). (eaglebio.com)
The Anti-Beta 2 GP1 ELISA is an enzyme immunoassay for the quantitative determination of IgG or IgM antibodies to ß2 glycoprotein-I in human serum or plasma. (eaglebio.com)

Coarse-grained simulations effectively capture antibodies embedding into membranes. (elifesciences.org)
B-cells producing antibodies targeting host membranes are downregulated in healthy organisms 1 - 4 . (elifesciences.org)
These drugs block receptor-mediated endocytosis, possibly by their actions on phospholipid metabolism [ 3355 ] or by covalent coupling to cellular membranes [ 3356 ]. (nanomedicine.com)
Essential fatty acid status in cell membranes and plasma of patients with primary Sjogren's syndrome. (omega-research.com)
Lipid-poor HDL precursors mature by obtaining cholesterol and phospholipids from cell membranes or triglyceride-rich lipoproteins. (jci.org)

Engineered mutations to the 10E8 paratope at light-chain solvent-exposed residues that add positive charge or hydrophobic sidechains proved to increase antibody association to anionic phospholipid vesicles in vitro and also boosted neutralization potency 27 . (elifesciences.org)
LAC is a functional assay that is used to detect APL antibodies that have anticoagulation activity in vitro. (medscape.com)
Recombinant antibodies are monoclonal, but their production involves in vitro genetic manipulation. (cellsignal.com)
In vitro methods for producing antibodies are amenable to large-scale production, meaning antibody availability is unlikely to become a limiting factor. (cellsignal.com)

1 The Venereal Disease Research Laboratory (VDRL) test is a manual agglutination assay that detects antibodies to cardiolipin extracted from bovine heart tissue. (dobratechnologia.pl)
The aCL assay can detect antibodies of differing specificities. (diapharma.com)
What is the advantage of an assay for anti-phospholipid antibody cofactors over an assay for the antibody itself? (diapharma.com)
Instrument effect on the activated protein C resistance plasma assay performed by a commercial kit. (ntu.ac.uk)
At CST, we adhere to the Hallmarks of Antibody Validation™ , six complementary strategies for determining the specificity, sensitivity, and functionality of an antibody in any given assay. (cellsignal.com)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Phospholipid Transfer Protein (PLTP) in serum, plasma, tissue homogenates and other biological fluids. (elisareagents.com)
Values for aCL antibodies are reported in GPL, MPL, and APL units, with assay cutoffs established at 23 GPL, 11 MPL, and 22 APL. (diapharma.com)

This case demonstrates a transient antiphospholipid antibody syndrome as a rare presentation of bleeding diathesis in a previously healthy child, and should be considered in children with new onset bruising and prolonged activated partial thromboplastin time. (bmj.com)
A low plasma HDL cholesterol level (HDL, high-density lipoprotein) is a common lipid abnormality in patients affected by premature CHD and also a component of the metabolic syndrome, a cluster of risk factors for atherosclerosis associated with central obesity. (oulu.fi)
The cardiolipin antibodies blood test, IgA, IgG, and IgM is used to help investigate inappropriate blood clot formation, to help determine the cause of recurrent miscarriage, or as part of an evaluation for antiphospholipid syndrome or sometimes other autoimmune diseases. (dobratechnologia.pl)
Anticardiolipin antibodies are often present in individuals with the antiphospholipid antibody syndrome. (dobratechnologia.pl)
The antiphospholipid syndrome (APS) is diagnosed in patients with recurrent thromboembolic events and/or pregnancy loss in the presence of persistent laboratory evidence for antiphospholipid antibodies. (dobratechnologia.pl)
these antibodies show a higher correlation with thrombosis and are more specific for the antiphospholipid syndrome. (diapharma.com)
Intro Beside clinical symptoms, the analysis of antiphospholipid syndrome (APS) is based on the presence of persistent antiphospholipid antibodies (aPL). (nanoker-society.org)
It�s a major phospholipid binding protein and an important component to measure in the assessment of anti-phospholipid syndrome. (assaygenie.com)
Per antiphospholipid syndrome diagnostic criteria, these anti-β 2 -glycoprotein I antibodies of IgG and/or IgM isotype should be present in the in plasma, in a titer greater than the 99th percentile to be considered a positive test result. (medscape.com)
In this update, the author discusses advancements in the epidemiology, diagnosis, treatment, and prognosis of patients with arterial ischemic stroke and cerebral venous thrombosis associated with genetic and acquired thrombophilia, including the antiphospholipid antibody syndrome. (medlink.com)
antiphospholipid antibody syndrome is the most common acquired disorder. (medlink.com)
In antiphospholipid antibody syndrome, pregnancy morbidity, including spontaneous abortions or preterm delivery due to placental insufficiency, is another clinical manifestation. (medlink.com)
Antiphospholipid syndrome (APS), or antiphospholipid antibody syndrome (APS, or SAFA), is an autoimmune condition of hypercoagulation accompanied by the appearance of antibodies to phospholipids. (lab24.pl)
Antibodies to β-2-glycoproteins belong to the class of antiphospholipid antibodies and were included in the laboratory classification criteria for the diagnosis of antiphospholipid syndrome in 2006 (Miyakis et al. (lab24.pl)
Antiphospholipid syndrome is an acquired autoimmune disorder characterized by recurrent arterial or venous thrombosis and/or pregnancy losses, in the presence of persistently elevated levels of anticardiolipin antibodies and/or evidence of circulating lupus anticoagulant (these abnormalities are detected by blood tests). (endinglines.com)
Antiphospholipid syndrome (APS) is caused by the body's immune system producing abnormal antibodies called antiphospholipid antibodies. (endinglines.com)
In 41 primary Sjögren's syndrome patients we compared fatty acid levels within erythrocyte phospholipids, plasma phospholipids, plasma triglycerides and plasma cholesterol esters, with the immunopathological and clinical disease status. (omega-research.com)

These peptide usually contains the epitope recognized by the antibody. (affbiotech.com)
Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. (affbiotech.com)
Mouse anti Human CD142 antibody, clone TF9-10H10 recognizes an epitope within the extracellular domain, epitope locus I. It recognizes both the reduced and native non-reduced human and primate tissue factors. (bio-rad-antibodies.com)
Genetic, structural, and functional characterization of a multi-donor class of 'public' antibodies revealed an NTD epitope that is recurrently mutated among emerging SARS-CoV-2 variants of concern. (cdc.gov)

When the antibodies attack cardiolipin, blood clots can occur. (dobratechnologia.pl)
It's not clear why these abnormal antibodies are produced, or why many people have antiphospholipid antibodies but don't develop blood clots. (endinglines.com)

ANGPTL3 inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels. (nsjbio.com)
E06-scFv was secreted into the plasma from the liver and macrophages, and achieved sufficient plasma levels to inhibit in vivo macrophage uptake of OxLDL and to prevent OxPL-induced inflammatory signalling. (fondationleducq.org)
Because the E06-scFv lacks the functional effects of an intact antibody other than the ability to bind OxPL and inhibit OxLDL uptake in macrophages, these data support a major proatherogenic role of OxLDL and demonstrate that OxPL are proinflammatory and proatherogenic, which E06 counteracts in vivo. (fondationleducq.org)
Compared to other vaccine formulations, immunization using CoPoP liposomes admixed with recombinant RBD induces multiple orders of magnitude higher levels of antibody titers in mice that neutralize pseudovirus cell entry, block RBD interaction with ACE2, and inhibit live virus replication. (cdc.gov)

The determination of IgA antibodies seems to have a greater validity in thrombosis and fetal loss. (rapidtest.com)
Cardiolipin antibodies (CA) are seen in a subgroup of patients with autoimmune disorders, particularly Systemic Lupus Erythematosus (SLE), who are at risk for vascular thrombosis, thrombocytopenia, cerebral infarct and/or recurrent spontaneous abortion. (dobratechnologia.pl)
Antibodies to Cardiolipin are reported to be associated with spontaneous thrombosis and thrombotic episodes and also with spontaneous abortion and placental infarction. (dobratechnologia.pl)
The serologic detection of anti-b2GPI antibodies provides enhanced clinical sensitivity for thrombosis. (diapharma.com)
Antibodies directed against b2GPI have been shown to be specific markers for thrombosis in individuals with systemic lupus erythematosus (SLE) and lupus-like disorders (APS). (diapharma.com)

Recently, we have developed high purity single molecule PEGs especially for Antibody Drug Conjugates (ADCs) to increase hydrophilicity and payload of drugs. (dds-drug.com)

A cardiolipin antibody test detects cardiolipin antibodies in the blood. (dobratechnologia.pl)
Realtime driving directions to GiFi, Av. A cardiolipin antibody test detects cardiolipin antibodies in the blood. (dobratechnologia.pl)
Cardiolipin antibodies present in the sample will bind to the coated wells. (diapharma.com)

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. (thermofisher.com)
LCAT, the major player in HDL maturation, is mainly synthesized by the liver but also in the brain, suggesting a crucial role of this enzyme in the maturation of brain lipoproteins, although LCAT concentration in the cerebrospinal fluid (CSF) is much lower than in plasma [ 12 ]. (biomedcentral.com)
Color develops in the wells at an intensity proportional to the serum concentration of aCL antibodies. (diapharma.com)
The concentration of antibodies of the specimen is directly read off the standard curve. (eaglebio.com)
3 vials, 0.250 ml (1-high, 2-moderate, 3-low) (human), see vial label for antibody concentration in APL units. (diapharma.com)

Monovariate logistic regression evaluation demonstrated a optimistic dose-effect relationship for anti-annexin V IgM with VOC, with elevated VOC danger seen with elevated antibody titers. (ncbcs.org)

21% of the samples were positive for IgG, 10% for IgM and 26% for IgA antibodies with the REAADS aCL assays. (diapharma.com)

Diluted patient serum or plasma, and calibrators, are added to the wells. (rapidtest.com)

The Anti Beta2GP1 specific antibodies, if present, bind to the antigens. (rapidtest.com)
The intensity of the color generated is proportional to the amount of IgA specific antibodies in the sample. (rapidtest.com)
Unfortunately, inducing a the advantages and disadvantages of an antibody-based protective response by vaccination may take longer than the defense strategy, and proposes stockpiling specific antibodies time between exposure and onset of disease. (cdc.gov)

Western blot of HRP anti-α-Synuclein, 80-96 antibody (clone A15115A). (biolegend.com)
IHC staining of HRP anti-α-Synuclein, 80-96 antibody (clone A15115A) on formalin-fixed paraffin-embedded normal (left panel) and Parkinson's disease (right panel) human brain tissues. (biolegend.com)
Jurkat T cells were left untreated (upper left & lower left panels), treated for 5 hours (upper middle & lower middle panels) or 12 hours (upper right & lower right panels) with anti-human Fas antibody (clone DX2, Cat. (bdbiosciences.com)

The bound antibodies react specifically with anti-human-IgG or IgM conjugated to horseradish peroxidase (HRP) within the next incubation period of 30 min at RT. (eaglebio.com)
The optical density (OD) of the solution at 450 nm is directly proportional to the amount of specifically bound antibodies.The standard curve is established by plotting the antibody concentrations of the standards (x-axis) and their corresponding OD-values (y-axis) measured. (eaglebio.com)

We investigated the prevalence of anti-annexin V IgG and IgM antibodies in sera of scleroderma sufferers and their relation to the presence of different antibodies and improvement of illness morbidity. (ncbcs.org)

With the ban on sub-therapeutic antibiotic usage in Europe and the increasingly strictness of the European legislation on food hygiene, passive immunization by oral administration of pathogen-specific hen egg yolk antibody (IgY) may be a useful and attractive alternative. (ac.be)
This review offers summarized information about IgY production and the use of these antibodies for passive immunization, particularly in poultry. (ac.be)
Thus, biological immunity is passive immunization with protective antibody. (cdc.gov)

10,000/μL) plasma preparation via ultracentrifugation must be done with special care, as it may cause platelet fragmentation and the release of phospholipids, which may significantly affect the results. (medscape.com)
The time required for the appearance of FIBRIN strands following the mixing of PLASMA with phospholipid platelet substitute (e.g., crude cephalins, soybean phosphatides). (lookformedical.com)

HDL originates as discoidal complexes of apolipoproteins and phospholipids. (jci.org)

AD patients have normal plasma lipids but significantly reduced unesterified cholesterol and unesterified/total cholesterol ratio. (biomedcentral.com)

Intravenous immunoglobulin (IVIG) is a therapeutic preparation of polyspecific antibodies isolated from pools of plasma obtained from several thousand healthy blood donors [1]. (academie-medecine.fr)

Mixing studies in plasma were positive for phospholipid dependence of the anticoagulant, confirming a diagnosis of lupus anticoagulant. (bmj.com)
Is administering one Anti-Phospholipid test sufficient for diagnosis? (diapharma.com)
Although significance and pathological relevance of phospholipid anti-bodies are not completely revealed yet, the detection of several autoantibody specificities is usually applied to the differential diagnosis and follow-up of systemic rheumatic inflammatory diseases. (eaglebio.com)

Nonetheless, in cases of chronic inflammation and infection as in HIV, rare antibodies with lipid affinity can emerge 5 - 8 . (elifesciences.org)
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions. (biolegend.com)

In light of the fact that lupus anticoagulants, a type of antiphospholipid antibody, have similar anticoagulant properties, the relationship of beta 2GPI to lupus anticoagulant activity was investigated. (jci.org)

Lipoprotein profile, cholesterol esterification, and cholesterol efflux capacity (CEC) were evaluated in plasma and CSF. (biomedcentral.com)
In agreement with the reduced preβ-HDL particles, cholesterol efflux capacity mediated by the transporters ABCA1 and ABCG1 was reduced in AD patients' plasma. (biomedcentral.com)
Recent studies have demonstrated that ATP-binding cassette protein A1 (ABCA1) is highly regulated in macrophages and mediates the efflux of cholesterol and phospholipids to apolipoproteins, a process necessary for HDL formation. (jci.org)
ABCA1 is expressed in many organs and is thought to mediate HDL maturation by facilitating the efflux of cellular phospholipids and cholesterol onto apolipoproteins. (jci.org)

An amino acid sequence from the N-terminus of human Angiopoietin-Like 3 (EPKSRFAMLDDVKILAN) was used as the immunogen for this ANGPTL3 antibody (100% homologous in human, mouse and rat). (nsjbio.com)

What is a Recombinant Antibody and Why is it Important? (cellsignal.com)
Recombinant antibodies offer several key advantages compared to traditional antibodies. (cellsignal.com)
As such, recombinant antibodies are seeing increased use for scientific research, especially as a means of addressing the ongoing reproducibility crisis. (cellsignal.com)
What is a Recombinant Antibody? (cellsignal.com)
Mammalian cell lines are most commonly used for recombinant antibody production, although cell lines of bacterial, yeast, or insect origin are also suitable. (cellsignal.com)
Because recombinant antibody production involves sequencing the antibody light and heavy chains, it is a highly controlled and reliable process. (cellsignal.com)
Recombinant antibodies are highly consistent from lot to lot, thereby ensuring reproducible experimental results. (cellsignal.com)
Unlike traditional methods for antibody production, recombinant approaches avoid the need to use animals. (cellsignal.com)
Where polyclonal antibodies are purified directly from the serum of the immunized host, and monoclonals are purified from either hybridoma-derived tissue culture supernatant or ascites, recombinant antibodies are instead purified from the tissue culture supernatants of transfected host cell lines. (cellsignal.com)
Regardless of whether an antibody is polyclonal, monoclonal or recombinant, it must always be properly validated in the intended application prior to experimental use. (cellsignal.com)
Here, it is shown that rapid conversion of recombinant RBD into particulate form via admixing with liposomes containing cobalt-porphyrin-phospholipid (CoPoP) potently enhances the functional antibody response. (cdc.gov)

Older patients who received unpurified plasma‐derived clotting factor concentrates may have signs and symptoms of infectious disease (eg, hepatitis, HIV infection). (medscape.com)

A attainable co-appearance of anticardiolipin (aCL), anti-β2-glycoprotein I (anti-β2-GPI), anti-prothrombin (aPT) and anti-annexin V (aANXV) antibodies of IgG, IgM and IgA class have been studied in 58 sufferers with SLE alone and 32 sufferers APS within the view of rational laboratory diagnostics. (ncbcs.org)
It is converted to thrombin by a prothrombin activator complex consisting of factor Xa, factor V, phospholipid, and calcium ions. (lookformedical.com)
Heat- and storage-labile plasma glycoprotein which accelerates the conversion of prothrombin to thrombin in blood coagulation. (lookformedical.com)

This article proposes a biological defense initiative based on developing, producing, and stockpiling specific antibody reagents that can be used to protect the population against biological warfare threats. (cdc.gov)

The clinical significance of these antibodies must be assessed in conjunction with the patient's symptoms, clinical history, and other laboratory findings. (diapharma.com)
With the clinical prerequisites of the presence of APS and a negative analysis for antibodies to cardiolipin and lupus anticoagulant. (lab24.pl)

Anatomy19

Organisms13

Diseases1

Chemicals and Drugs130

Analytical, Diagnostic and Therapeutic Techniques and Equipment51

Phenomena and Processes40

Disciplines and Occupations1

Technology, Industry, Agriculture9

Information Science4

Health Care13

Negatively charged8

Dilute Russ2

Binds5

Beta-2 glycoprotein14

Proteins13

Cofactor8

Membrane15

Autoantibodies2

Anticardiolipin antibody4

Autoimmune disease3

Antigen6

Epitopes3

Anti-Cardiolipin8

Monoclonal antibodies3

Reactivity2

ELISA9

Membranes5

Vitro4

Assay7

Syndrome17

Epitope4

Blood clots2

Inhibit4

Thrombosis5

Conjugates1

Cardiolipin Antibodies3

Concentration5

Titers1

Assays1

Serum or plasma1

Specific antibodies3

Clone3

Bound antibodies2

Morbidity1

Passive immunization3

Platelet2

Complexes1

Lipids1

Immunoglobulin1

Diagnosis3

Affinity2

Anticoagulant properties1

Efflux4

Immunogen1

Recombinant11

Infectious disease1

Prothrombin3

Reagents1

Clinical2