• Currently, studies are focussing on the design of plant virus-derived vectors to achieve higher amounts of transiently expressed proteins in these systems. (cas.cz)
  • Here we designed and tested replicating single and multi-cassette vectors that combine elements for enhanced replication and hypertranslation, and assessed their ability to express and particularly co-express proteins by Agrobacterium-mediated transient expression in tobacco BY-2 plant cell packs. (cas.cz)
  • Substantial yields of green and red fluorescent proteins of up to ~ 700 ng/g fresh mass were detected in the plant cells along with position-dependent expression. (cas.cz)
  • This is the first evidence of the ability of replicating vectors to transiently express proteins in BY-2 plant cell packs. (cas.cz)
  • We have generated a novel tool for the expression of recombinant proteins in plants and for the functional analysis of virus and plant genes. (biomedcentral.com)
  • Here, we demonstrate that non-infectious SARS-CoV-2 VLPs can be successfully assembled by co-expressing three important viral proteins membrane (M), envelop (E) and nucleocapsid (N) in plants. (nature.com)
  • Expression vectors are the basic tools in biotechnology for the production of proteins. (wikipedia.org)
  • Other fusion proteins such as green fluorescent protein may act as a reporter gene for the identification of successful cloned genes, or they may be used to study protein expression in cellular imaging. (wikipedia.org)
  • The expression host of choice for the expression of many proteins is Escherichia coli as the production of heterologous protein in E. coli is relatively simple and convenient, as well as being rapid and cheap. (wikipedia.org)
  • 19. Mara C D,Huang T,Irish V F. The Arabidopsis floral homeotic proteins APETALA3 and PISTILLATA negatively regulate the BANQUO genes implicated in light signaling[J].Plant Cell,2010,22(3):690-702. (nefu.edu.cn)
  • R1- To k now, understand and apply the molecular underpinnings and biotechnological techniques in the production of recombinant proteins in plants. (unavarra.es)
  • 1 - Plants as chemical and pharmaceutical factories: production of therapeutic proteins, antibodies, vaccines, enzymes and proteins of industrial use. (unavarra.es)
  • Faye L y Gomord V. Recombinant proteins from plants. (unavarra.es)
  • Fischer R y Schillberg S. Molecular farming: plant-made pharmaceuticals and technical proteins. (unavarra.es)
  • Eukaryotic expression vectors containing genes encoding plant proteins for killing of cancer cells. (weeksmd.com)
  • Proteins of interest (POI) are tagged with a GFP and transiently expressed in plant cells to serve as donor fluorophore. (nature.com)
  • To illustrate our approach, we provide experimental procedures to detect the interaction between plant DNA and two proteins (the AeCRN13 effector from the oomycete Aphanomyces euteiches and the AtWRKY22 defensive transcription factor from Arabidopsis ). (nature.com)
  • Figure 8: GFP lifetime distribution of GFP:AeCRN13 and GFP:AeCRN13 AAA fusion proteins in plant nuclei. (nature.com)
  • Meanwhile, FZ supported systemic expression of fluorescent proteins mNeonGreen and mCherry in N. benthamiana and soybean. (researchsquare.com)
  • The objective of this research is to determine the biochemical functions of Hessian fly effectors and their interacting proteins in wheat plants. (usda.gov)
  • Next, we will generate recombinant proteins using the cloned genes that have shown a measurable plant response. (usda.gov)
  • A subset of plant intracellular NLR immune receptors detect effector proteins, secreted by phytopathogens to promote infection, through unconventional integrated domains which resemble the effector's host targets. (elifesciences.org)
  • Engineering NLR proteins to improve disease resistance in crop plants is a major goal of the field. (elifesciences.org)
  • A variety of techniques have been used to examine plant viral genomes, the functions of virus-encoded proteins, plant responses induced by virus infection and plant-virus interactions. (springer.com)
  • This overview considers these technologies and how they have been used to identify novel viral and plant proteins or genes involved in disease and resistance responses, as well as defense signaling. (springer.com)
  • Our vectors apply positive selection for the insert or negative selection against empty vector molecules and drive strong expression of target proteins in E.coli cells. (biomedcentral.com)
  • This position of the promoter permits positive selection and attenuated expression of fusion proteins with C-terminal tags. (biomedcentral.com)
  • In the following, automated cloning and expression screening was conducted with six different target proteins which were known to us to be well expressed. (biomedcentral.com)
  • Thus, striking improvements in recombinant antigen were achieved by alternative polyadenylation signals and fusion proteins containing targeting signals designed to enhance integration or retention of HBsAg in the endoplasmic reticulum (ER) of plant cells [6]. (scielo.br)
  • (Empty Backbone) Overexpression of recombinant proteins in plants thanks to very efficient translational efficiency. (addgene.org)
  • Besides being the first heterologous expression and characterization of a gene coding for a lipase from A. alcalophilum , this report shows that LipA is very versatile exhibiting both acetylxylan esterase and lipase activities potentially useful for diverse industry sectors, and that tobacco is a suitable bioreactor for producing fungal proteins. (biomedcentral.com)
  • We have developed a novel method for co-expressing multiple chimeric fluorescent fusion proteins in plants to overcome the difficulties of conventional methods. (jove.com)
  • This method can help answer key questions in the plant molecular and cell biology field such as, how can we proteins in the cell. (jove.com)
  • The main advantage of this technique is the high efficiency of co-expressing cellular fusion proteins in one expression vector. (jove.com)
  • Polyclonal antibodies against the recombinant Canecystatins were raised, allowing the immunodetection of the endogenous proteins in the plant tissues. (unifesp.br)
  • To take advantage of homology‐directed repair, we developed modular vectors to rapidly generate DNA donor plasmids to efficiently introduce DNA sequences encoding for fluorescent proteins at the 5′ and 3′ ends of gene coding regions. (nsf.gov)
  • This course reviews applied aspects of molecular biology, production of heterologous proteins in insect cells (so called Baculovirus Expression Vector System) cereal plant biotechnology, and drug development. (lu.se)
  • An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for gene expression in cells. (wikipedia.org)
  • An expression vector must have elements necessary for gene expression. (wikipedia.org)
  • Gene expression however may also be constitutive (i.e. protein is constantly expressed) in some expression vectors. (wikipedia.org)
  • Familiarity with molecular biology, plant gene expression, and transformation vector technologies is preferred. (publicgardens.org)
  • Moreover, transient gene expression through viral vectors allowed the accumulation of carotenoids outside the plastid. (frontiersin.org)
  • We will use changes in the gene expression levels of Mds-1 and the lipid transfer protein gene BT008922 as biomarkers to measure plant responses using RT-qPCR. (usda.gov)
  • To understand the differential gene expression in Anopheles following infection with drug-resistant Plasmodium , we conducted a genome-wide transcriptomic profiling analysis of Anopheles stephensi following feeding on mice with drug-resistant or drug-sensitive P. yoelii , observed changes in gene expression profiles and identified transcripts affected by the drug-resistant parasite. (biomedcentral.com)
  • The use of recombinant DNA technologies is nowadays spread through most laboratories conducting research in life sciences and the applications, including gene expression systems, tend towards being more parallelized. (biomedcentral.com)
  • QU Jing, GUO Xing-qi, CI Xiao-yan, QI Dong, WEN Fu-jiang*.Recent Advances on M olecular Biology of Potato virus X and Its Application to Gene Expression Vector .VIROLOGICA SINICA, 2003, 18(1): 87-92. (virosin.org)
  • Use of this method to target doublesex (a gene involved in sex differentiation) or fork head (which encodes a salivary gland transcription factor) resulted in reduced target gene expression and/or protein immunofluorescence signal, as measured by quantitative Real-Time PCR (qRT-PCR) or fluorescence confocal microscopy, respectively. (cdc.gov)
  • We sought to better understand larval SG architecture, secretion, and gene expression. (cdc.gov)
  • Meta-analysis of microarray data showed that larval SG gene expression is divergent from adult SGs, more similar to larval gastric cecae, but different from other larval gut compartments. (cdc.gov)
  • Christou P y Klee H. Handbook of plant biotechnology (volume 2). (unavarra.es)
  • Position Summary: Contribute to the objectives of the Daniel P. Haerther Charitable Trust New Plant Development Program through the development of in vitro propagation and application of biotechnology to the breeding, selection, and research of woody landscape plants for built environments. (publicgardens.org)
  • Qualifications: Bachelor's degree required, Master's degree preferred in plant tissue culture and biotechnology or a related science field, and 2+ years of related research or work experience required with a demonstrated knowledge of research design, implementation, and analysis and an ability to conduct independent research. (publicgardens.org)
  • Lomonossoff, G.P. Cowpea mosaic virus: the plant virus-based biotechnology workhorse. (eurekaselect.com)
  • Canonical CRISPR-Cas9 genome editing technique has profoundly impacted the fields of plant biology, biotechnology, and crop improvement. (nsf.gov)
  • However, CRISPR‐Cas9‐mediated genome editing in P. patens was shown to be more efficient than traditional homologous recombination (Plant Biotechnology Journal, 15, 2017, 122). (nsf.gov)
  • Over one hundred vaccines using varied approaches including protein subunit, viral vector, RNA, DNA, inactivated virus, attenuated virus, and virus like particles (VLPs) platforms are in clinical trials. (nature.com)
  • Among these, viral vector and RNA vaccines are currently in use. (nature.com)
  • With helpful illustrations, photos, figures, models that explain viral mechanisms, and easy-to-understand reference tables, Plant Viruses As Molecular Pathogens will stimulate your thinking on this fascinating area of plant science! (routledge.com)
  • These approaches include analysis of spatial and temporal responses by plants to infection, and techniques that allow the expression of viral genes transiently or transgenically in planta, the expression of plant and foreign genes from virus vectors, the silencing of plants genes, imaging of live, infected cells, and the detection of interactions between viral proteins and plant gene products, both in planta and in various in vitro or in vivo systems. (springer.com)
  • Schaffer, D.V. Viral Vectors for Gene Therapy: Translational and Clinical Outlook. (eurekaselect.com)
  • HBsAg was the first viral antigen chosen to be produced in transgenic plants, firstly in tobacco [3] and subsequently in lupin callus and lettuce adapted to colder climates [4]. (scielo.br)
  • Dr. Dugan was an assistant professor of viral genomics at the J. Craig Venter Institute from 2010 to 2012, where she focused on influenza and vector-borne viral genomics, viral evolution and synthetic influenza vaccine development. (cdc.gov)
  • Recently, a book chapter with protocols for the Baculovirus Expression Vector System in insect cells was published by LP3. (lu.se)
  • Lund Protein Production Platform (LP3) has recently published its standard operating procedures for recombinant protein expression using the Baculovirus Expression Vector System (BEVS) in insect cells. (lu.se)
  • Early vectors suffered from limitations such as instability and low yields, but this has been addressed by the genetic modification of vector sequences and by delivering virus vectors into plant cells using Agrobacterium tumefaciens [ 39 , 40 ]. (biomedcentral.com)
  • 17. Yanofsky M F. Floral meristems to floral organs:genes controlling early events in Arabidopsis flower development[J].Annu Rev Plant Physiol Plant Mol Biol,1995,46:167-188. (nefu.edu.cn)
  • Results: Data addressing the function of vectors harbouring genes specifically encoding ricin, saporin, lunasin , linamarase, and tomato thymidine kinase 1 under the control of different promoters are summarised here. (weeksmd.com)
  • The new CPSMV-derived FZ vector provides a convenient and versatile soybean functional genomics tool that is expected to accelerate the characterization of soybean genes controlling crucial productivity traits. (researchsquare.com)
  • This, combined with the relatively small numbers of seed each soybean plant produces, makes it extremely challenging to generate mutant seed libraries that cover even 50% of genes. (researchsquare.com)
  • The insect can be controlled through plant host resistance, but the insect population typically overcomes new resistance genes within a few years after deployment. (usda.gov)
  • 14. Gowda, S., Wu, F.C., Scholthof, H.B., and Shepherd, R.J. (1989) Gene VI of figwort mosaic virus (caulimovirus group) functions in posttranscriptional expression of genes on the fulllength RNA transcript. (springer.com)
  • An alternative approach is to use plants as bioreactors, transferring the corresponding genes from microorganisms into plants and producing them at economically acceptable levels [ 8 ]. (biomedcentral.com)
  • To date, only few cystatin genes have been characterized in most plant species. (unifesp.br)
  • Seeds of rice lines that had T-DNA insertions in regions corresponding to individual putative GalT genes were obtained from a Rice Functional Genomic Express Database and plants grown until maturity. (bvsalud.org)
  • This highly flexible, user-friendly, low-cost, time-efficient method of dsRNA delivery could be broadly applicable to target genes important for insect vector physiology and beyond. (cdc.gov)
  • The occurrence of RIP genes has been described in a wide range of plant taxa, as well as in several species of bacteria and fungi. (cdc.gov)
  • We found that CP replacement vectors were unable to move systemically and that vectors with duplicated SGPs (even heterologous SGPs) suffered from significant transgene instability. (biomedcentral.com)
  • Further, VLPs have the advantage that, contrary to inactivated or attenuated viruses that must be prepared in mammalian cell lines, they can be produced in heterologous systems, such as bacteria, yeasts, insect cells, or plants. (nature.com)
  • Furthermore, pVNPs can be produced rapidly and inexpensively in natural host plants or heterologous production platforms. (eurekaselect.com)
  • or (f) living vectored cells expressing specific heterologous antigens. (who.int)
  • The plasmid vector PV-GHGT07 contains well characterized DNA segments required for selection and replication of the plasmid in the bacteria as well as a right border for initiating the region of T-DNA, into the plant genomic DNA. (cbd.int)
  • It takes advantage of using a single expression plasmid that contains multiple functionally independent protein expressing cassettes to achieve protein co-expression. (jove.com)
  • 3. Davies, J.W. and Hull, R. (1982) Genome expression of plant positive-strand RNA viruses. (springer.com)
  • Our Drosophila Cas9 expression pUASTB vector system has the capability to utilize either the Drosophila P-element transposon system (like pUAST) or the bacteriophage φC31 integration system (like pUASTattB) for Cas9 insertion into the genome. (vectorbuilder.kr)
  • This system also incorporates a user-defined promoter to achieve ubiquitous, tissue-specific or inducible Cas9 protein expression. (vectorbuilder.kr)
  • Utilizing the CRISPR/Cas9 system in Drosophila allows the rapid generation of knockout lines by simply delivering either an all-in-one vector (a single vector expressing both Cas9 and gRNA) or separate vectors for driving Cas9 and gRNA expression, respectively. (vectorbuilder.kr)
  • To fully take advantage of CRISPR‐Cas9 genome editing in P. patens , here we describe the generation and use of a flexible and modular CRISPR‐Cas9 vector system. (nsf.gov)
  • Some examples include delivering DNA-free gene editing reagents such as ribonucleoproteins or mRNA, relying on reagent expression from non-integrated DNA, using novel delivery mechanisms such as viruses or nanoparticles, using unconventional selection methods to avoid integration of transgenes, and/or avoiding tissue culture altogether. (frontiersin.org)
  • 0 - Transgenic plants: concept and methods of plant transformation. (unavarra.es)
  • Peyret et al Plant Methods. (addgene.org)
  • Construction of Plant Expression Vector and Genetic Transformation Analysis of Arabidopsis thaliana AtPI Gene in Nicotiana tabacum [J]. Bulletin of Botanical Research, 2016, 36(3): 388-394. (nefu.edu.cn)
  • Complementation of plant mutants with large genomic DNA fragments by a transformation-competent artificial chromosome vector accelerates positional cloning[J].Proc Natl Acad Sci USA,1999,96(11):6535-6540. (nefu.edu.cn)
  • Plant transformation techniques are well developed for making transgenic varieties in certain crops and model organisms, yet reagent delivery and plant regeneration remain key bottlenecks to applying the technology of gene editing to most crops. (frontiersin.org)
  • Typical plant transformation protocols to produce transgenic, genetically modified (GM) varieties rely on transgenes, chemical selection, and tissue culture. (frontiersin.org)
  • The successful candidate will primarily work with woody landscape plants for built environments, specializing in plant tissue culture, embryo rescue, and transformation technologies. (publicgardens.org)
  • Develop and implement novel approaches to embryo rescue and plant nuclear transformation. (publicgardens.org)
  • Tools for chloroplast transformation in Chlamydomonas: expression vectors and a new dominant selectable marker. (bio.net)
  • We have established an automated, fast and flexible low-cost expression cloning approach requiring only vector and insert amplification by PCR and co-transformation of the products. (biomedcentral.com)
  • Here we report a strategy for genetic transformation of lettuce plants (Lactuca sativa L.) using the surface antigen HBsAg of hepatitis B virus. (scielo.br)
  • These vectors are designed for protein transient expression and genetic transformation. (jove.com)
  • In many crops, genome engineering techniques have enabled researchers and breeders to take advantage of a vast knowledge base of plant physiology, pathology, and genetics. (frontiersin.org)
  • Motion, G.B., Howden, A.J.M., Huitema, E. & Jones, S. DNA-binding protein prediction using plant specific support vector machines: validation and application of a new genome annotation tool. (nature.com)
  • We describe the development of a new vector based on cowpea severe mosaic virus (CPSMV), a plus-strand RNA virus with its genome divided into two RNA segments, RNA1 and RNA2. (researchsquare.com)
  • The φC31 integrase mediates irreversible recombination between attB and attP sites, resulting in the linearization and integration of the pUASTB vector into the host genome. (vectorbuilder.kr)
  • For this reason, the attB vector system is designed to be used with Drosophila lines carrying attP "landing sites" within their genome. (vectorbuilder.kr)
  • For Expression in plants, transform Agrobacterium tumefaciens strain LBA4404 and grow at 28C. (addgene.org)
  • Here we describe how to detect nucleic acid-protein interactions in plant leaves by using a fluorescence resonance energy transfer (FRET) approach coupled to fluorescence lifetime imaging microscopy (FLIM). (nature.com)
  • To develop strategies for more effective, durable resistance, the molecular foundations of the plant-insect interactions need to be better understood. (usda.gov)
  • The work is of interest to colleagues in synthetic biology, protein engineering and plant-pathogen interactions. (elifesciences.org)
  • 11. Theissen G,Saedler H. Plant biology. (nefu.edu.cn)
  • The last decade has seen remarkable advances in plant virological research, owing mainly to the rapid progress made in molecular biology and genetic engineering in recent years. (routledge.com)
  • Plant viruses are useful expression vectors because they can mount systemic infections allowing large amounts of recombinant protein to be produced rapidly in differentiated plant tissues. (biomedcentral.com)
  • While recombinant DNA technology has significantly contributed to our understanding of plant viruses, new findings are being accumulated every day as reported in various publications. (routledge.com)
  • Plant Viruses As Molecular Pathogens is the only book to bring you all of this information--22 chapters--in a single volume, compiled by specialists around the globe! (routledge.com)
  • 2. Zaitlin, M. and Palukaitis, P. (2000) Advances in understanding plant viruses and virus disease. (springer.com)
  • The proteinaceous structure of plant viruses allows the capsid structure to be modified by genetic engineering and/or chemical conjugation with nanoscale precision. (eurekaselect.com)
  • Furthermore, we described the versatility of plant viruses, with innate immunostimulatory properties, in providing a huge natural resource of carriers that can be used to develop the next generation of sustainable vaccines. (eurekaselect.com)
  • The obtainment of transgenic edible plants carrying recombinant antigens is a desired issue in search for economic alternatives viewing vaccine production. (scielo.br)
  • We explored several strategies for vector development including coat protein (CP) replacement, duplication of the CP subgenomic promoter (SGP) and the creation of a fusion protein using the foot-and-mouth disease virus (FMDV) 2A catalytic peptide. (biomedcentral.com)
  • The vector is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. (wikipedia.org)
  • The promoter initiates the transcription and is therefore the point of control for the expression of the cloned gene. (wikipedia.org)
  • The insert contains the CoMV promoter region, a codon optimised cp4epsps coding sequence, nptII, aad (that is not expressed in plants) and a portion of ori-V coding regions. (cbd.int)
  • another competent vector for the Italian 2017 chikungunya virus strain ( 8 ), is largely not present in continental Europe with the exception of a small region around the eastern coast of the Black Sea. (cdc.gov)
  • An attractive alternative is to administer transient gene silencing or overexpression with a plant virus-based vector. (researchsquare.com)
  • However, existing virus-induced gene silencing (VIGS) and/or overexpression vectors suitable for soybean have various drawbacks that hinder their widespread adoption. (researchsquare.com)
  • Pepino mosaic virus (PepMV) (genus Potexvirus , family Flexiviridae ), a widespread plant virus, is a promising candidate expression vector for plants because of its high level of accumulation in its hosts and the absence of severe infection symptoms. (biomedcentral.com)
  • 4 - Post-translational modifications of biopharmaceutical compounds produced in plants. (unavarra.es)
  • This review highlights diverse strategies to deliver CRISPR/Cas gene editing reagents to regenerable plant cells and to recover edited plants without unwanted integration of transgenes. (frontiersin.org)
  • Numerous vectors have been engineered for the sole purpose of killing cancer cells, and some have successfully suppressed malignant tumours. (weeksmd.com)
  • Cells co-transfected with an estrogen response element (ERE)-driven luciferase (Luc) reporter gene and an ERalpha- or ERbeta-expression vector were exposed to graded concentrations of ellagic acid. (nutrimedical.com)
  • We provided experimental data for the applications of Variable-Angle Epifluorescence Microscopy (VAEM) in dissecting protein dynamics in plant cells. (biomedcentral.com)
  • The present study presented experimental evidence on illustrating the use of VAEM in tracking and dissecting protein dynamics, dissecting endosomal dynamics, cell structure assembly along with membrane microdomain and protein motility in intact plant cells. (biomedcentral.com)
  • Without exception, all single-molecule studies on plant cells using TIRF, VAEM or Highly Inclined and Laminated Optical sheet (HiLo) microscopy [ 11 ] are conducted based on this principle. (biomedcentral.com)
  • Production of the fusion polypeptide involves the introduction of the hybrid gene into a biological host cell system, for example yeast cells, which permits the expression and accumulation of the fusion polypeptide. (justia.com)
  • E. coli, P. pastoris , Mammalian cells, Plant cells and in vitro by cell free synthesis. (lu.se)
  • This vector, designated FZ, incorporates a cloning site in the RNA2 cDNA, permitting insertion of nonviral sequences. (researchsquare.com)
  • We have tested eight vectors by inserting six target sequences of variable length, provenience and function. (biomedcentral.com)
  • JEV multi-epitope peptide (MEP) sequences fused with FLAG-tag or 6× His-tag at the C- or N-terminus for the purification were introduced into plant expression vectors and used for transient expression. (bvsalud.org)
  • Among the constructs, vector pSK480, which expresses MEP fused with a FLAG-tag at the C-terminus, showed the highest level of expression and yield in purification. (bvsalud.org)
  • In plants, they are synthesized in plastids where they play essential roles in light-harvesting and in protecting the photosynthetic apparatus from reactive oxygen species (ROS). (frontiersin.org)
  • During this SARS-CoV-2 pandemic, many studies on the development of plant-derived vaccines and antibodies are underway. (nature.com)
  • In order to explore the function of PI gene in the development of petals and stamens, the PI gene in Arabidopsis thaliana was used as the research object, and the AtPI gene was amplified from cDNA by PCR, and the plant expression vector(pROKⅡ- AtPI ) was constructed and transformed into tobacco. (nefu.edu.cn)
  • Transgenic tobacco plants exist obvious phenotype compared to wild-type tobacco, such as smaller corolla, shorter stamens, and abnormal ovary, which is 5-10 mm longer than wild-type tobacco. (nefu.edu.cn)
  • An expression vector has features that any vector may have, such as an origin of replication, a selectable marker, and a suitable site for the insertion of a gene like the multiple cloning site. (wikipedia.org)
  • In the rapidly evolving field of nanotechnology, plant virus nanoparticles (pVNPs) are emerging as powerful tools in diverse applications ranging from biomedicine to materials science. (eurekaselect.com)
  • The promoters used in expression vector are normally inducible, meaning that protein synthesis is only initiated when required by the introduction of an inducer such as IPTG. (wikipedia.org)
  • and inducible promoters such as Mtn and DmHsp70 for achieving expression in the presence of Cu+ and in response to heat stress, respectively. (vectorbuilder.kr)
  • In addition, recent studies revealed the option of synthetic conversion of leaf chloroplasts into chromoplasts, thus increasing carotenoid storage capacity and boosting the nutritional value of green plant tissues. (frontiersin.org)
  • Some vectors may include targeting sequence that may target the expressed protein to a specific location such as the periplasmic space of bacteria. (wikipedia.org)
  • Low level of constitutive protein synthesis may occur even in expression vectors with tightly controlled promoters. (wikipedia.org)
  • Plants are being considered as an alternative vaccine/mAb production system, especially in unexpected or emergency situations that cannot be solved by existing systems. (nature.com)
  • We will use the pEAQ-HT-DEST1 protein transient expression vector system to screen for candidate effectors that can trigger measurable plant responses. (usda.gov)
  • The vector pSelAct-KO is a recombination based, markerless knockout system that uses dual selection to engineer seamless deletions of a region of interest, providing opportunities for repeated higher-order genetic knockouts. (biorxiv.org)
  • Three mutation prediction tools (inDelphi, FORECasT, and SPROUT) have been validated in the rice plant system. (nsf.gov)
  • Without the need for gene synthesis, this vector system enables editing of up to 12 loci simultaneously. (nsf.gov)
  • This study used a transient expression system to express the chimeric protein composed of antigenic epitopes from the JEV envelope (E) protein in Nicotiana benthamiana. (bvsalud.org)
  • A stable PepMV vector was generated by expressing the transgene as a CP fusion using the sequence encoding the foot-and-mouth disease virus (FMDV) 2A catalytic peptide to separate them. (biomedcentral.com)
  • systems to optimize the expression of the transgene. (unavarra.es)
  • Additionally, the mini white gene on the pUAST vector encodes eye color and acts as a marker for the identification of transgenic flies which have undergone successful integration of the transgene. (vectorbuilder.kr)
  • Carotenoids are well-known isoprenoid pigments naturally produced by plants, algae, photosynthetic bacteria as well as by several heterotrophic microorganisms. (frontiersin.org)
  • Here, we briefly describe carotenoid biosynthesis and highlight the latest advances and discoveries related to synthetic carotenoid metabolism in plants and microorganisms. (frontiersin.org)
  • Vectors used for protein production in organisms other than E.coli may have, in addition to a suitable origin of replication for its propagation in E. coli, elements that allow them to be maintained in another organism, and these vectors are called shuttle vectors. (wikipedia.org)
  • Plants as factories for protein production. (unavarra.es)
  • Flowering of strict photoperiodic Nicotiana varieties in non-inductive conditions by transgenic approaches[J].Plant Mol Biol,2007,65(3):233-242. (nefu.edu.cn)
  • Here, we focus on nontransgenic RNAi-based approaches that use oral delivery of dsRNA through feeding of inactivated bacteria to produce RNAi in disease vectors and in a crop pest. (cdc.gov)
  • Direct binding of effectors to these integrated domains activates plant defenses. (elifesciences.org)
  • First, compared to most crop plants, these plants are relatively easy to transform. (researchsquare.com)
  • Interestingly, manipulation of carotenoid biosynthesis can also influence plant architecture, and positively impact growth and yield, making it an important target for crop improvements beyond biofortification. (frontiersin.org)
  • This vector has expanded its geographic range through increasing global trade of used tires and plants and established itself in areas with suitable climate and habitat in many parts of the world. (cdc.gov)
  • Some insects cause harm by vectoring diseases dangerous to humans, livestock, or plants or by damaging crops. (cdc.gov)
  • Byron Breedlove] Linking microbiology, infectious diseases, epidemics, and various vectors to art, and vice versa, creates perspectives that can help humanize the science and offer a different way of seeing the art. (cdc.gov)
  • The Morton Arboretum is seeking an in vitro biologist to join their plant development team, which focuses on studying horticultural crops, their taxonomy, stressors, and ways to improve their performance in urban environments. (publicgardens.org)
  • 2008. A plant virus movement protein forms ringlike complexes with the major nucleolar protein, fibrillarin, in vitro . (hutton.ac.uk)
  • The expression of a protein may be tightly controlled, and the protein is only produced in significant quantity when necessary through the use of an inducer, in some systems however the protein may be expressed constitutively. (wikipedia.org)
  • Reverse genetics research in some plants that were adopted as early model systems, including Arabidopsis, rice, and tomato, is relatively straightforward for several reasons. (researchsquare.com)
  • The term 'negative selection' is used here to describe cloning systems with vectors that contain the ccdB cell-death gene which is replaced by the cloned insert, i.e . the selection eliminates the vector molecules without insert. (biomedcentral.com)
  • With its work, the Leibniz Institute of Vegetable and Ornamental Crops (IGZ) contributes to a better understanding of plant systems and thus to the development of sustainable and resilient horticulture. (leibniz-gemeinschaft.de)
  • Although lipases are known to occur in a diversity of organisms, including animals, plants and microbes, the current industrial production of these enzymes relies almost exclusively on microbial-based expression systems. (biomedcentral.com)
  • Next, linearize the final protein expression backbone vector POC 18, and vector CAMBIA 1300, by adding four units of small one into a final 10 microliter reaction volume. (jove.com)