Alkaline phosphatase, placental type also known as placental alkaline phosphatase (PLAP) is an allosteric enzyme that in humans is encoded by the ALPP gene. (wikipedia.org)
There are at least four distinct but related alkaline phosphatases: intestinal (ALPI), placental (this enzyme), placental-like (ALPPL2), and liver/bone/kidney (ALPL) (tissue-nonspecific). (wikipedia.org)
Alkaline phosphatase, placental type is a membrane-bound glycosylated dimeric enzyme, also referred to as the heat-stable form, that is expressed primarily in the placenta, although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. (wikipedia.org)
The complete amino acid sequence and three-dimensional structure of human placental alkaline phosphatase have been reported. (accesson.kr)
N-glycan structures from human placental alkaline phosphatase (PLAP) were investigated using HILIC-Orbitrap MS, and subsequent data processing and glycan assignment software. (accesson.kr)
Monoclonal antibody assay of serum placental alkaline phosphatase in the monitoring of testicular tumours. (ox.ac.uk)
Extreme elevation of placental alkaline phosphatase as a marker of preterm delivery, placental insufficiency and low birth weight. (nel.edu)
Laboratory tests showed elevated (as much as a 10.5-fold increase) values of alkaline phosphatase enzyme, 94.05% of which was placental isoenzyme. (nel.edu)
The authors point out the potential relationship between elevated placental isoenzyme of alkaline phosphatase levels and placental insufficiency and the onset of a preterm delivery. (nel.edu)
[ 2 ] Aminotransferase levels and bilirubin are unaffected by pregnancy, although maternal alkaline phosphatase levels may increase by up to three times normal from placental production. (medscape.com)
The tumor cells were negative for glial fibrillary acidic protein (GFAP), placental alkaline phosphatase (PLAP), Human Chorionic Gonadotropin (HCG), and pan-cytokeratin. (thieme-connect.com)
One hybridoma, producing the monoclonal antibody H7, against placental alkaline phosphatase was adopted to offer CDR-containing regions for new recombinant constructs. (projectsparadise.com)
Quantitative PCR was performed for relative telomere length (RTL) analyses, while expression of relevant genes for pluripotency markers, differentiation state (Cbfa1, human placental alkaline phosphatase, peroxisome proliferator activated receptor, Sox9 and Collagen II a1), and telomerase reverse transcriptase (hTERT) was determined by semiquantitative RT-PCR. (mendeley.com)
Placental alkaline phosphatase (PLAP) positive EVs increase with gestational age and may be relatively increased early in pregnancy in women who develop severe pregnancy complications. (selectbiosciences.com)
Using human placental alkaline phosphatase (hPLAP) as a genetic marker for cell tracking, we examined the therapeutic efficacy and the homing of intravenously administered, hPLAP labeled cells in marker tolerant, immunocompetent rats after induction of MI. (uni-muenchen.de)
Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. (teomics.com)
2P-275] Three-dimensional visualization of tissue structures in transgenic Xenopus embryos using a placental alkaline phosphatase reporter and COMBI system. (atlas.jp)
PLAP1
Importantly, PLAP alone is not specific for placental EVs and multiplex antibody labeling is required to measure cell-specific events in plasma. (selectbiosciences.com)
Circulation1
During the 2nd trimester, blood pressure (BP) usually drops (and pulse pressure widens), even though CO and renin and angiotensin levels increase, because uteroplacental circulation expands (the placental intervillous space develops) and systemic vascular resistance decreases. (msdmanuals.com)
Term placental3
We have prepared monoclonal antibodies by immunizing BALB/c mice with purified human term placental plasma membranes. (ox.ac.uk)
In this study, primary cytotrophoblasts (CTBs) from early pregnancy and primary human placenta-derived mesenchymal stem cells (hPDMSCs) from different stages of pregnancy were selected as the cell research objects, and full-term placental tissue was selected as the tissue research object to detect the effects of their conditioned medium (CM) on human umbilical vein endothelial cell (HUVEC) angiogenesis. (biomedcentral.com)
This study's objective was to investigate the role of hypoxia on normal term placental pericytes (PLVP) and their signaling to endothelial cells. (bvsalud.org)
Secreted placental alkaline phosphatase1
The way we know that it worked was because when the subject had the right thought, the gene in the mouse that encodes an enzyme called SEAP (secreted placental alkaline phosphatase) got activated. (extremetech.com)
Alkaline phosphatase gene1
4. Transcriptional regulation of the human placental-like alkaline phosphatase gene and mechanisms involved in its induction by sodium butyrate. (nih.gov)
Monoclonal antibodies3
Preparation and characterization of monoclonal antibodies against placental alkaline phosphatase and other human trophoblast-associated determinants. (ox.ac.uk)
Production of monoclonal antibodies to placental alkaline phosphatase: preliminary characterisation includes identification of one antibody reactive with routinely fixed histological preparations. (ximbio.com)
Monoclonal antibodies to placental alkaline phosphatase: preclinical evaluation in a human xenograft tumour model of F(ab')2 and Fab fragments. (ximbio.com)
Human6
Retinal ganglion cells and axons are revealed by staining for human placental alkaline phosphatase (purple) in Pou4f2 AP knock-in mice. (jneurosci.org)
Toward this end, they engineered mice that expressed a reporter gene (human placental alkaline phosphatase) under the control of the ARE promoter. (alzforum.org)
A sensitive and selective method was developed for the first time to quantify simultaneously the normal and formaldehyde (FA)-modified bases in human placental DNA treated with 100 ppm FA for 20 h at 37 degrees C. Digestion of DNA to deoxynucleosides with DNase I, phosphodiesterase and alkaline phosphatase occurred in that order with centrifugation steps. (cdc.gov)
The respective concentrations were about 6.0, 10.0 and 23 pmol of modified deoxynucleosides in 80 ug of human placental DNA after treatment with 100 µg/mL of formalin for 20 h at 37 degrees C. The stabilities of N6-dA and N2-dG were much better at -20 degrees C than at 25 degrees C, where the respective halftimes were about 50.1 and 21.0 h. (cdc.gov)
Pericytes are multifunctional cells wrapped around capillary endothelia, essential for vascular health, development, and blood flow regulation, although their role in human placental chorionic villi has not been fully explored. (bvsalud.org)
TNF diffused into the alginate capsules, bound to the specific TNF receptors on the transgenic designer cells' surface and triggered the expression of the reporter gene SEAP (human placental secreted alkaline phosphatase) that was rewired to the TNF-specific signaling cascade. (pubchase.com)
Gene2
SensoLyte® pNPP Secreted Alkaline Phosphatase Reporter Gene Assay Kit provides a sensitive colorimetric assay of placental alkaline phosphatase for both secreted and membrane-bound form of enzyme. (mobitec.com)
From NCBI Gene: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). (nih.gov)
Membranes2
Methimazole readily crosses placental membranes and can cause fetal harm, particularly when administered in the first trimester of pregnancy. (nih.gov)
Because the drug readily crosses placental membranes, methimazole can cause fetal goiter and cretinism when administered to a pregnant woman. (nih.gov)
Fetal3
Gestational trophoblastic neoplasms (GTN) are a group of neoplasms from fetal trophoblastic cells including choriocarcinoma (CC), epithelioid trophoblastic tumors (ETT), and placental site trophoblastic tumors (PSTT) [ 1 ]. (hindawi.com)
It is well known that placental villi consist of different cell types: (1) trophoblasts (syncytiotrophoblasts and cytotrophoblasts), (2) mesenchymal cells (mesenchymal stem cells, fibroblasts, mesenchymal-derived macrophages-Hoffbauer cells), and (3) fetal vascular cells (vascular smooth muscle cells, vascular pericytes, and endothelial cells) [ 4 ]. (biomedcentral.com)
The second half of normal pregnancy is characterized by a progressive decline in placental and fetal oxygen levels which, by term, comprises a substantial degree of hypoxia. (bvsalud.org)
Phosphatases1
There are at least four distinct but related alkaline phosphatases: intestinal (ALPI), placental (this enzyme), placental-like (ALPPL2), and liver/bone/kidney (ALPL) (tissue-nonspecific). (wikipedia.org)
Chorionic1
The determination of biochemical markers in the blood: chorionic gonadotropin (hCG), alpha-fetoprotein (AFP) and placental alkaline phosphatase (PCP) is of auxiliary importance in the diagnosis of germinoma and in the assessment of the condition of the disease. (medic-journal.com)
Membrane-bound1
Alkaline phosphatase, placental type is a membrane-bound glycosylated dimeric enzyme, also referred to as the heat-stable form, that is expressed primarily in the placenta, although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. (wikipedia.org)
Liver1
Although about 20 cases of PTU induced hepatic damage were reported in the medical literature, this is, as far as we know, the first description of neonatal liver injury probably caused by placental transfer of this drug. (nih.gov)
Antibody3
One antibody recognized transferrin, a serum protein with an abundant placental receptor. (ox.ac.uk)
Furthermore, we measured the levels of angiogenic factors in the CM of placental cells or tissue by an angiogenesis antibody array. (biomedcentral.com)
The semiquantitative angiogenesis antibody array showed that 18 of the 43 angiogenic factors had obvious spots in placental cell-derived CM or sub-cultured placental tissue-derived CM, and the levels of 5 factors (including CXCL-5, GRO, IL-6, IL-8, and MCP-1) were the highest in sub-cultured placental tissue-derived CM. (biomedcentral.com)
Maternal2
Causes can be intrinsic (to the fetus) or extrinsic (maternal or placental problems). (wikidoc.org)
The oxidative stress caused by under-perfusion leads to the release of circulating factors (placental syncytial fragments), which induce a maternal inflammatory response that subsequently causes endothelial cell dysfunction, triggering widespread vasoconstriction and capillary leakage. (pharmaceutical-journal.com)
Vitro3
We successfully isolated primary hPDMSCs and CTBs, collected CM from these placental cells and sub-cultured placental tissue, and then evaluated the effects of the CM on a series of angiogenic processes in HUVECs in vitro. (biomedcentral.com)
The results showed that not only placental cells but also sub-cultured placental tissue, to some extent, promoted HUVEC angiogenesis in vitro by promoting proliferation, adhesion, migration, invasion, and tube formation. (biomedcentral.com)
CM obtained from placental cells (primary CTBs or hPDMSCs) or sub-cultured placental tissue contained proangiogenic factors and promoted HUVEC angiogenesis in vitro. (biomedcentral.com)
Tissue5
There has been no systematic, comparative study on the roles of these two groups of placental cells and the whole placental tissue in the placental angiogenesis. (biomedcentral.com)
Placental cell-derived CM collected at 24 h or 48 h had the best effect, and sub-cultured placental tissue-derived CM collected at 7 days had the best effect among all the different time points. (biomedcentral.com)
Therefore, our research is helpful to better understand placental angiogenesis regulation and provides theoretical support for the clinical application of placental components, especially sub-cultured placental tissue-derived CM, in vascular tissue engineering and clinical treatments. (biomedcentral.com)
G) Mediastinal soft tissue mass demonstrates positive immunohistochemistry for placental alkaline phosphatase and (H) CD 117. (neurology.org)
The placenta is fundamental to its pathogenesis and releases placental factors as well as extracellular vesicles (small and medium/large syncytiotrophoblast extracellular vesicles (STB-EVs)) as a response to syncytiotrophoblast stress such as tissue factor and plasminogen activator inhibitors 1. (bvsalud.org)
Levels2
During the 2nd trimester, blood pressure (BP) usually drops (and pulse pressure widens), even though CO and renin and angiotensin levels increase, because uteroplacental circulation expands (the placental intervillous space develops) and systemic vascular resistance decreases. (msdmanuals.com)
Pregnant females have increased levels due to placental secretion of ALP. (ruh.nhs.uk)
Cells3
Placental vascular development is complex and delicate and involves many types of placental cells, such as trophoblasts, and mesenchymal stem cells. (biomedcentral.com)
We also found that primary placental cells in early pregnancy, whether CTBs or hPDMSCs, played more significant roles than those in full-term pregnancy. (biomedcentral.com)
Therefore, proper development of the placental vasculature depends on the autocrine and paracrine signals of vascular cells and other types of placental cells [ 9 ]. (biomedcentral.com)