• Pichia pastoris , a methylotrophic yeast, is our favourite host, which we employ for expression and secretion of recombinant soluble proteins as well as for the biosynthesis of valuable metabolites, e.g. plant terpenoids, through heterologous expression of membrane proteins. (tugraz.at)
  • The methylotrophic yeast, Pichia pastoris , has been genetically engineered to produce many heterologous proteins for industrial and research purposes. (pacific.edu)
  • Over the last few decades the methylotrophic yeast Pichia pastoris has become a popular host for a wide range of products such as vaccines and therapeutic proteins. (biomedcentral.com)
  • In recent years the methylotrophic yeast Pichia pastoris has received wide acceptance for the production of biopharmaceuticals [ 3 ]. (biomedcentral.com)
  • Among these alternative expression systems, Pichia pastoris was chosen as a proven methylotrophic (i.e. methanol-utilising) heterologous expression system. (edu.au)
  • All Cosmetics of the KIROS COSMETICS line contain an innovative fermented extract of Pichia Pastoris, a methylotrophic yeast belonging to the Ascomycetes class. (kirosdiet.com)
  • With strong potential for being an expression system for protein production, as well as being a model organism for genetic study, Komagataella phaffii has become important for biological research and biotech applications. (wikipedia.org)
  • Swichkow CR, Bloom JS, Kruglyak L. ( 2022 ) Exploring the genetic architecture of protein secretion in Komagataella phaffii (Pichia pastoris) for biotechnology applications. (academictree.org)
  • Pichia pastoris (Komagataella phaffii) is widely used for industrial production of heterologous proteins due to high secretory capabilities but selection of highly productive engineered strains remains a limiting. (biomedcentral.com)
  • Understanding K. phaffii (Pichia pastoris) Host Cell Proteins: Proteomic Analysis and Flow-through Affinity Clearance (T. I. Williams, Ed.). Biotechnology and Bioengineering, Vol. 01. (ncsu.edu)
  • The earliest biochemical evidence on the existence of cell-free soluble LH receptor was the purification of an hCG-binding protein, relative molecular mass of 65K (M r , 65K) from porcine follicular fluid and was based on gel filtration followed by affinity chromatography [ 9 ]. (biomedcentral.com)
  • 11 ] first reported the production and secretion of soluble LH receptor following transient transfection of a naturally truncated variant of the receptor in COS cells. (biomedcentral.com)
  • Therefore, how a complex LHCGR protein, embedded within the membrane lipid bilayer, could be secreted as soluble receptor remained unexplained. (biomedcentral.com)
  • Decelerating translation elongation speed has been shown to improve the soluble protein yield when expressing heterologous proteins in industrial expression hosts. (biomedcentral.com)
  • With purified protein and soluble substrates, the exoglucanase activity of the GH7 domain embedded in the Fusion 3 construct in L. starkeyi was significantly higher than that of the GH7 domain in TeTr CBHI expressed alone. (biomedcentral.com)
  • We offer over 30 years of combined experience in the manufacturing of recombinant proteins following all expression solutions, i.e. periplasmic expression, soluble cytoplasmic expression, secretion and inclusion bodies refolding. (xpress-biologics.com)
  • In 1995, P. pastoris was reassigned into the sole representative of genus Komagataella, becoming Komagataella pastoris. (wikipedia.org)
  • According to GBIF: Komagataella kurtzmanii G.I.Naumov, E.S.Naumova, Tyurin & Kozlov, 2013 Komagataella mondaviorum G.I.Naumov, E.S.Naumova & K.L.Boundy-Mills, 2018 Komagataella pastoris (Guillierm. (wikipedia.org)
  • The complete genomic data allows scientists to identify homologous proteins and evolutionary relationships between other yeast species and Komagataella. (wikipedia.org)
  • Furthermore, Komagataella are single eukaryotic cells, which means researchers could investigate the proteins inside Komagataella. (wikipedia.org)
  • Moreover, unlike S. cerevisiae, Komagataella has the ability to functionally process proteins with large molecular weight, which is useful in a translational host. (wikipedia.org)
  • Due to its abilities of recombination with foreign DNA and processing large proteins, much research has been carried out to investigate the possibility of producing new proteins and the function of artificially designed proteins, using Komagataella as a transformation host. (wikipedia.org)
  • Komagataella is frequently used as an expression system for the production of heterologous proteins. (wikipedia.org)
  • New approaches to high throughput protein, isolation, purification and concentration. (ukri.org)
  • As well proving a range of expression and purification facilities, PTL can offer a GLP-compliant bioprocess services to produce recombinant proteins. (protein-technologies.com)
  • Towards continuous mAb purification: Clearance of host cell proteins from CHO cell culture harvests via "flow-through affinity chromatography" using peptide-based adsorbents. (ncsu.edu)
  • This yeast has the ability to produce and secrete large quantities of protein in the cultivation medium, which facilitates the recovery of the biological material and significantly decreases the contamination with host cell proteins and facilitates the purification. (xpress-biologics.com)
  • Baculovirus expression is a powerful and versatile system for producing high levels of recombinant protein expression in insect cells. (protein-technologies.com)
  • DESCRIPTION (provided by applicant): With its ability to secrete high levels of recombinant proteins, the yeast Pichia pastoris is extensively used for protein manufacturing. (sbir.gov)
  • In Phase I of this project a strategy was developed and tested using the model protein alpha 1-antitrypsin for overcoming bottlenecks in yeast secretion that result in less than optimal yields. (sbir.gov)
  • Taken with the present results, this suggests that the potential use of inhibitors of prolyl 4-hydroxylase to control excessive collagen deposition in pathological fibrosis may be limited by the need to maintain continuous inhibition of collagen hydroxylation so as to facilitate intracellular degradation of the accumulated protein. (embl.de)
  • The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. (antikoerper-online.de)
  • Escherichia coli is widely used host for the intracellular expression of many proteins. (sharif.edu)
  • Improvement of both intracellular and extracellular production of recombinant protein in E. coli is an attractive goal in order to reduce production cost and increase process efficiency and economics. (sharif.edu)
  • Since heat shock proteins in E. coli were reported to be helpful for protein refolding and hindering aggregation, in this work different types of single and periodic heat shocks were tested on lab scale to enhance intracellular and extracellular protein production. (sharif.edu)
  • The secreted proteins were folded and sensitive to glycosidases suggesting N-linked glycosylation. (biomedcentral.com)
  • Unlike the bacterial expression systems, which lack the modification enzymes, Pichia pastoris is able to produce heterologous proteins with post-translational modifications, especially glycosylation, which is crucial for optimal properties of many pharmaceutical proteins. (biomedcentral.com)
  • However, as actual yields vary widely for individual proteins, GlycoFi is focusing on the improvement of protein yields in P. pastoris as part of the company's long term mission to develop yeast-based protein production technology for manufacturing therapeutic proteins with human glycosylation structures. (sbir.gov)
  • Proposed Commercial Application: GlycoFi's Humanized Yeast(tm) enables the study of glycosylation structure-function relationships and creates the ability not only to pinpoint the specific glycoforms that optimize the bioactivity, pharmacokinetics and overall efficacy of a therapeutic protein, but also to manufacture them at low cost with a high degree of uniformity. (sbir.gov)
  • It is of particular importance in processing protein s for secretion , containing a set of glycosylation enzyme s that attach various sugar monomers to proteins as the proteins move through the apparatus. (explained.today)
  • A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. (antikoerper-online.de)
  • Here, a large-scale deletion library of ribosomal protein (RP) genes was constructed for heterologous protein expression in Pichia pastoris , and 59% (16/27) RP deletants have significantly increased heterologous protein yield. (biomedcentral.com)
  • A combinatorial library/high throughput screening methodology was designed to optimize translocation of the nascent peptide into the endoplasmic reticulum (ER) and protein folding within the ER. (sbir.gov)
  • In this study, using a combination of the bioinformatics design and molecular genetics, the protein A signal peptide from Staphylococcus aureus was modified, redesigned and then fused to the mature hGH coding region. (biomedcentral.com)
  • Since the native signal protein peptide of S. aureus protein A was not able to deliver hGH to the extracellular space, it was modified using bioinformatics tools and fused to the n-terminal region of hGh to show that the redesigned signal peptide was functional. (biomedcentral.com)
  • Development of Peptide Ligands for Targeted Capture of Host Cell Proteins from Cell Culture Production Harvests. (ncsu.edu)
  • Removal of host cell proteins from cell culture fluids by weak partitioning chromatography using peptide-based adsorbents. (ncsu.edu)
  • Targeted capture of Chinese hamster ovary host cell proteins: Peptide ligand binding by proteomic analysis. (ncsu.edu)
  • DNA sequence alignment analysis and the forecast-analysis of protein structure indicated that these seven antimicrobial peptide genes were not homologous to any other antimicrobial peptide genes in GenBank. (ukessays.com)
  • Though some mutations clearly dampened protein expression, deletion of amino acids 57-70, corresponding to the predicted 3rd alpha helix of α-mating factor secretion signal, increased secretion of reporter proteins horseradish peroxidase and lipase at least 50% in small-scale cultures. (pacific.edu)
  • Pancreatic lipase-related protein 2 (PLRP2) has been identified as an important factor of galactolipid digestion in humans, together with pancreatic carboxyl ester hydrolase (CEH). (inra.fr)
  • The kinetic properties of TTL were compared to those of the homologous lipase from Thermomyces lanuginosus (TLL), guinea pig pancreatic lipase-related protein 2 and Fusarium solani cutinase. (inra.fr)
  • The concept of recombinant protein from recombinant DNA harnessed the world's most miniature manufacturing factories, living cells. (springeropen.com)
  • Pichia pastoris is a widely used platform for heterologous protein expression, which is a "generally regarded as safe" (GRAS) microorganism. (biomedcentral.com)
  • Although overexpression of the unfolded protein response regulator Hac1p has been widely used to enhance protein secretion, its effects on the physiology of host cells were often overlooked, which would attenuate and even neutralize its beneficial effects on overall protein production. (bvsalud.org)
  • We have uncovered several endogenous yeast proteins and mechanisms that render protein secretion or membrane protein expression more efficient and sustainable. (tugraz.at)
  • We have identified a novel isoform of the pig liver esterase protein family, markedly improved expression of pig liver esterases in P. pastoris and E. coli, and have evolutionarily engineered pig liver esterases for biocatalytic applications. (tugraz.at)
  • Expression systems for lactic acid bacteria have been developed for metabolic engineering applications as well as for food-grade recombinant protein production. (biomedcentral.com)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • Moreover, the lack of a secreted form of the receptor was overcome by co-expression of hCG with the extra-cellular domain of porcine LH receptor leading to the secretion of a mixture of free as well as hCG-receptor complex [ 13 ]. (biomedcentral.com)
  • A potential expression platform for efficient pharmaceutical proteins and industrial enzymes production was provided without synonymous mutation. (biomedcentral.com)
  • We offer a custom specific high-level recombinant protein coli expression service using a variety of commercially available strains. (protein-technologies.com)
  • Expression optimization, flask and bioreactor production, rapid scale up to 200 L/batch, growth monitoring, protein refolding and tag removal as requested and full cGMP production. (protein-technologies.com)
  • The advantages of insect cell expression are that it offers posttranslational modifications similar to mammalian cells with high levels of protein expression and ease of scale-up. (protein-technologies.com)
  • PTL offers a high-level algal expression service from research to industrial grade recombinant proteins with high growth rates. (protein-technologies.com)
  • PTL can offer a novel Agrobacterium infiltration-mediated transient expression for a rapid and highly efficient method for protein expression in plants. (protein-technologies.com)
  • Using tobacco plant expression the secretion, post-translational modifications and high expression of recombinant proteins ranging from recombinant enzymes to therapeutic targets and vaccines can be achieved using this system. (protein-technologies.com)
  • In Phase II, yield optimization studies will include IgG as well as alpha 1-antitrypsin with specific experiments targeting expression and secretion of the IgG H and L chains. (sbir.gov)
  • The effect of α-mating factor secretion signal mutations on recombinant protein expression in Pichia pastoris. (pacific.edu)
  • Extensive site-directed mutagenesis of the prepro-region of the α-mating factor secretion signal sequence was performed in order to determine the effects of various deletions and substitutions on expression. (pacific.edu)
  • ImageJ analysis of the HIP's SDS-PAGE shows that the average HIP expression level of the recombinant P. pastoris truncated α-factor clone (CL4) was significantly higher compared to the full-length (HF7) when expressed in both media. (springeropen.com)
  • CL4 clone, which utilized a truncated α-factor in the P. pastoris HIP expression cassette, significantly expressed HIP 8.97 times (in BMMY) and 1.17 times (in BSMM) higher than HF7 clone, which used a full-length α-factor secretory signal. (springeropen.com)
  • This research confirmed that deletion of some regions of the secretory signal sequence significantly improved the efficiency of HIP protein expression in P. pastoris . (springeropen.com)
  • We report on the cloning and expression of a recombinant F. gigantica 14-3-3 epsilon protein (rFg14-3-3e), and testing its effects on specific functions of goat peripheral blood mononuclear cells (PBMCs). (biomedcentral.com)
  • Recent advances in genetic engineering have made it possible for microorganisms to be used for expression of heterologous proteins. (biomedcentral.com)
  • Several P. pastoris engineered strains and mutants have been developed to improve the performance of the expression system. (biomedcentral.com)
  • The success of the P. pastoris expression system is partly attributed to its ability to grow to high cell densities and as such increasing its volumetric productivity. (biomedcentral.com)
  • The promoter of the AOX1 gene has become a popular tool to drive the expression of recombinant proteins. (biomedcentral.com)
  • The Pichia pastoris expression system provides a cost-effective approach to overcoming the limitations of chemical synthesis and E. coli recombinant expression. (mdpi.com)
  • Our initial assays with the L. starkeyi secretome expressing differing Tr EGII domains fused to a chimeric Talaromyces emersonii - T. reesei CBHI ( TeTr CBHI) showed that the complete Tr EGII enzyme, including the glycoside hydrolase (GH) 5 domain is required for increased expression level of the fusion protein when linked to CBHI. (biomedcentral.com)
  • We found that this new construct ( Tr EGII- TeTr CBHI, Fusion 3) had an increased secretion level of at least threefold in L. starkeyi compared to the expression level of the chimeric TeTr CBHI. (biomedcentral.com)
  • To solve this problem, significant expression and secretion levels of heterologous cellulases in yeasts must be achieved. (biomedcentral.com)
  • Other expression systems also did not show satisfactory results regarding yields, the biological activity of the protein or economic viability. (edu.au)
  • In the fouth chapter, the aim was to study how selective pressure on the Histidinol dehydrogenase gene (HIS4), using amino acid starvation, affects the level of expression and secretion of the adjacent hIFNγ in the transformed P. pastoris GS115 strain, a histidine-deficient mutant. (edu.au)
  • As shown by ELISA, amino acid starvation-induced selective pressure on HIS4 improved expression and secretion of the adjacent hIFNγ by 55% compared to unchallenged cells. (edu.au)
  • Protein engineering is the purposeful modification of a protein's sequence, structure, or expression. (mdpi.com)
  • Co-expression of Ov-phy-1 with the O. volvulus homologue of protein disulfide isomerase in a baculovirus system resulted in the production of enzymatically active O. volvulus prolyl 4-hydroxylase. (embl.de)
  • The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. (antikoerper-online.de)
  • Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies. (antikoerper-online.de)
  • Results: For heterologous expression of abaecin in Pichia pastoris, we designed an ORF with HisTag, and the codon usage was optimized. (bocsci.com)
  • The new ORF was sub-cloned in the pPIC9 expression vector and transformed into P. pastoris. (bocsci.com)
  • Part of the parasite's wide-ranging virulence is attributed to the expression and secretion of large amounts of papain-like cysteine peptidases, namely cathepsin L and cathepsin B [ 3 ]. (biomedcentral.com)
  • Our goal was to develop a general and convenient strategy to improve heterologous protein synthesis in Pichia pastoris without mutating the expressed genes. (biomedcentral.com)
  • Global decrease in the translation elongation speed by RP deletion enhanced co-translational folding efficiency of nascent chains and decreased protein aggregates to improve heterologous protein yield. (biomedcentral.com)
  • However, unlike the endogenous proteins, little is known about the molecular mechanisms governing heterologous protein production. (bvsalud.org)
  • For example, green fluorescent protein (GFP) is a eukaryotic protein containing 11 beta-sheet structures and its folding yield was significantly increased by co-translational folding in E. coli [ 14 ]. (biomedcentral.com)
  • Another protein, phytase (Phy) from Citrobacter amalonaticus CGMCC 1696, has 95% homology to E. coli -derived phytase which consists of one α-domain containing five α-helices and a β-hairpin, and one α/β-domain including seven β-sheets and four α-helices [ 15 ]. (biomedcentral.com)
  • Escherichia coli and Pichia pastoris (single ou multicopy clone strategy). (xpress-biologics.com)
  • Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. (antikoerper-online.de)
  • We engineer the membranes of microbes, i.e. bacteria and yeasts, regarding lipid and membrane protein composition, either to understand the complex interactions of these hydrophobic compounds or to apply the modified microorganisms for interesting goals of industrial biotechnology. (tugraz.at)
  • For example, it can have inhibitory effects on glycolysis followed by a direct effect on protein synthesis [ 2 ], as well as increase the absorption and retention of calcium, magnesium and phosphate ions in the body. (biomedcentral.com)
  • The ciliary epithelium, a bilayer of secretory neuroepithelial cells, is the site of synthesis of aqueous humor (inflow system), and it regulates its secretion by the coordinated action of a complex transport system that involves ion channels and active transporters. (yale.edu)
  • The ciliary epithelium, a bilayer of secretory neuroepithelial cells, is the site of synthesis of aqueous humor (inflow system), and regulates its secretion by the coordinated action of a complex transport system involving ion channels and active ion secretion, from the stromal to the aqueous-humor surface, followed by osmotic water movement. (yale.edu)
  • As specialized, inducible cytoplasmic organelles, lipid droplets have functions beyond the regulation of lipid metabolism, in cell signalling and activation, membrane trafficking and control of inflammatory mediator synthesis and secretion. (microbiologyresearch.org)
  • Glypican-1 drives unconventional secretion of fibroblast growth factor 2. (nso-journal.org)
  • Key steps in unconventional secretion of fibroblast growth factor 2 reconstituted with purified components. (nso-journal.org)
  • Tec-kinase-mediated phosphorylation of fibroblast growth factor 2 is essential for unconventional secretion. (nso-journal.org)
  • The Na,K-ATPase acts upstream of phosphoinositide PI(4,5)P 2 facilitating unconventional secretion of Fibroblast Growth Factor 2. (nso-journal.org)
  • Accordingly, the cytokines produced by the KSiNP-treated macrophages were capable of inducing fibroblast proliferation and subsequent secretion of extracellular matrix (ECM). (megazyme.com)
  • Various assays were used to investigate the stimulatory effects of the purified rFg14-3-3e protein on specific functions of goat PBMCs, including cytokine secretion, proliferation, migration, nitric oxide (NO) production, phagocytosis, and apoptotic capabilities. (biomedcentral.com)
  • Also, this recombinant protein promoted the release of NO and cell apoptosis, and inhibited the proliferation and migration of goat PBMCs and suppressed monocyte phagocytosis. (biomedcentral.com)
  • Finally, we found that at least Xyn11A accumulated in the apoplast of the infected plant after three days, highlighting the role of these enzymes as important secreted proteins during fungal proliferation inside plant tissues. (omicsdi.org)
  • The molecular structure of Fasciola gigantica 14-3-3 protein has been characterized. (biomedcentral.com)
  • Overexpression of these genes can rescue temperature‐sensitive (ts) growth defect of many sec mutants impaired in protein secretion. (vtt.fi)
  • Furthermore, their overexpression in wild‐type yeast enhances production of secreted proteins in S. cerevisiae, which suggests that they may be rate‐limiting factors in this process. (vtt.fi)
  • In a simple example, one's favorite protein may be altered to facilitate the overexpression of a tagged version in yeast, which generates large quantities of yeast for in vitro investigations. (mdpi.com)
  • To foster plant terpenoid biosynthesis in yeasts, and to improve the industrial applicability of membrane-attached cytochrome P450 enzymes we have identified several endogenous helper factors in P. pastoris and S. cerevisiae. (tugraz.at)
  • This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. (cancerindex.org)
  • Over 5000 proteins were manufactured in P. pastoris (data from RCT Pichia technology), most of which are industrial enzymes and biopharmaceutical proteins [ 1 , 2 ]. (biomedcentral.com)
  • Dimou E, Nickel W. Unconventional mechanisms of eukaryotic protein secretion. (nso-journal.org)
  • What does this gene/protein do? (cancerindex.org)
  • What pathways are this gene/protein implicaed in? (cancerindex.org)
  • The Trichoderma reesei eg2 ( Tr EGII) gene was selected as the leading gene due to its previously demonstrated outstanding secretion in yeast. (biomedcentral.com)
  • hIFNγ was cloned into the pPIC9 vector adjacent to the HIS4 gene, a gene essential for histidine biosynthesis, which was then transformed into P. pastoris. (edu.au)
  • The protein encoded by the CALCRL gene is a G protein-coupled receptor related to the calcitonin receptor. (cgrpreceptor.com)
  • Drug moieties used in antibody drug conjugates include bacterial protein toxins such as diphtheria toxin, plant protein toxins such as ricin, small molecules such as auristatins, geldanamycin (Mandler et al (2000) J. of the Nat. (justia.com)
  • Steringer JP, Nickel W. A direct gateway into the extracellular space: Unconventional secretion of FGF2 through self-sustained plasma membrane pores. (nso-journal.org)
  • The production of recombinant mature hormone in suitable hosts and secretion of this therapeutic protein into the extracellular space can be considered as one of the best cost-effective approaches not only to obtain the active form of the protein but also endotoxin-free preparation. (biomedcentral.com)
  • Our particular focus in the yeast lipid field are sterols, their biosynthesis and homeostasis, and their interactions with membrane proteins. (tugraz.at)
  • Secretion was independent of recombinant size and was mediated via structurally defined membrane vesicles (50-150nm). (biomedcentral.com)
  • Brough D, Pelegrin P, Nickel W. An emerging case for membrane pore formation as a common mechanism for the unconventional secretion of FGF2 and IL-1β. (nso-journal.org)
  • [1] Part of the endomembrane system in the cytoplasm , it packages proteins into membrane-bound vesicle s inside the cell before the vesicles are sent to their destination. (explained.today)
  • The SacI-linearized plasmid pPICZαA-Stomoxyn -Zy was transformed into P. pastoris GS115 by electroporation. (ukessays.com)
  • We have developed a recombinant protein production platform and have produced more than 30 different proteins for therapeutic and imaging applications. (xpress-biologics.com)
  • We have applied multiple approaches to identify key genes that determine protein secretion rates in P. pastoris. (tugraz.at)
  • Upregulation of several target genes has improved recombinant protein titers and yields. (tugraz.at)
  • This is due to the delay of 60S subunit assembly by deleting non-essential ribosomal protein genes or 60S subunit processing factors, thus globally decreased the translation elongation speed and improved the co-translational folding, without perturbing the relative transcription level and translation initiation. (biomedcentral.com)
  • Interestingly, KlSSO1 and KlSEB1 show similar enhancing effect on production of a secreted protein as the SSO and SEB1 genes of S. cerevisiae when overexpressed. (vtt.fi)
  • String-based protein-protein interaction analysis suggested that bZIP53, a transcription factor might be involved in the activation of transcription of ELO / KCS genes. (nature.com)
  • However, due to the remarkable codon preference and tRNA content against the other species, heterologous proteins expressed in Pichia pastoris often encounter folding problem, which severely limited their production efficiency [ 3 ]. (biomedcentral.com)
  • In addition, the native sequence (NS) and two codon-optimised sequences (COS1 and COS2) for P. pastoris were used. (edu.au)
  • The encoded Kluyveromyces proteins are up to 70% identical with the S. cerevisiae homologues at the amino acid level and can functionally replace them. (vtt.fi)
  • Secretion is a common bottleneck in the production of industrial proteins. (bvsalud.org)
  • The results show that commercial production of low cost, eukaryotic recombinant hIFNγ is not an economically viable in P. pastoris. (edu.au)
  • Yield and quality of a recombinant product are important parameters to monitor during the host selection and development process but little information is published regarding quality differences of a product produced by different P. pastoris strains. (biomedcentral.com)
  • We showed that misincorporation of methoxine occurs particularly in P. pastoris Mut S strains. (biomedcentral.com)
  • Several strains and mutants of P. pastoris are described and available. (biomedcentral.com)
  • Primig, M.: Integrated RNA- and protein profiling of fermentation and respiration in diploid budding yeast provides insight into nutrient control of cell growth and development. (tu-dresden.de)
  • Naturally, proteins produced in eukaryotic cell have signal peptides that direct them to the export machinery so that the proteins can be secreted from the cell [ 11 ]. (springeropen.com)
  • The CGN is the first cisternal structure, and the TGN is the final, from which protein s are packaged into vesicle s destined to lysosome s, secretory vesicles, or the cell surface. (explained.today)
  • This control is propagated through the alpha-subunit of prolyl 4-hydroxylase where protein levels were previously shown to rise fivefold with increasing cell density. (embl.de)
  • Using a fluorescent sulfhydryl probe as well as a sulfhydryl inhibitor, one observes a strong cell density response, supporting the hypothesis that cellular redox potential could alter protein stability. (embl.de)
  • A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. (antikoerper-online.de)
  • Lipid droplet binding and oligomerization properties of the Parkinson's disease protein alpha-synuclein. (microbiologyresearch.org)
  • Autophagy's secret life: Secretion instead of degradation. (nso-journal.org)
  • To overcome this obstacle, we have explored the approach of genetically linking an easily secreted protein to CBHI, with CBHI being the last to be folded. (biomedcentral.com)
  • In this approach, heterologous proteins are expressed either as cytoplasmic or intracellularly or secreted as extracellularly [ 11 ]. (biomedcentral.com)
  • The low secretion levels of cellobiohydrolase I (CBHI) in yeasts are one of the key barriers preventing yeast from directly degrading and utilizing lignocellulose. (biomedcentral.com)
  • Bioengineered yeasts have also been used for vaccine development, reprogramming a protein's fate, directed evolution, and protein-based library screening. (mdpi.com)
  • The secretion of aqueous humor and the regulation of intraocular pressure are fundamental physiologic processes for the integrity and normal function of the mammalian eye. (yale.edu)
  • Rabouille C. Pathways of unconventional protein secretion. (nso-journal.org)