Phytase. Medical search. Frequent questions

A phytase (myo-inositol hexakisphosphate phosphohydrolase) is any type of phosphatase enzyme that catalyzes the hydrolysis of phytic acid (myo-inositol hexakisphosphate) - an indigestible, organic form of phosphorus that is found in many plant tissues, especially in grains and oil seeds - and releases a usable form of inorganic phosphorus. (wikipedia.org)
Most of the known phytases belong to a class of enzyme called histidine acid phosphatases (HAPs). (wikipedia.org)
These first examples of this class of enzyme were originally cloned from Bacillus species, but numerous microorganisms have since been identified as producing β-propeller phytases. (wikipedia.org)
PAPhy_a phytase is highly stable and a potent enzyme in feed. (frontiersin.org)
This pH optimum is the same as for Aspergillus ficuum phytase, a popular microbial feed enzyme. (frontiersin.org)
Each gram of UT PHOS contains E.Coli based Phytase enzyme, produce through submerged liquid fermentation with high yield gene engineering strain. (venkys.com)
van Tieghem, and expression of this gene produces the enzyme 3-phytase. (cbd.int)
We have developed an enzyme application strategy called Maximum Matrix Nutrition, which capitalises on the properties of Quantum Blue, an enhanced E. coli phytase with a high affinity for phytate, in addition to a thermostable and inhibitor-resistant xylanase, Econase XT. (abvista.com)
INTRODUCTION The phytase enzyme [EC 3.1.3.26] is a highly concentrated thermostable preparation produced by submerged fermentation of Aspergillus niger followed by purification and formulation. (enzymes.bio)
A quick review of the most recent book of the European Symposium on Poultry Nutrition (ESPN) abstracts reveals a continuing focus on the enzyme phytase . (feedstrategy.com)
The correct answer is that, yes, there are substantial differences among phytases, not only in the way they go about attacking the phytate entity, releasing phosphorus, but also in the amount of phosphorus they release and, of course, the origin of the enzyme that may affect not only its efficiency but also its technical quality aspects. (feedstrategy.com)
Corn contains approximately 0.26% phosphorus, but most of this phosphorus is bound to phytic acid, or phytate, and thus is not bioavailable to pigs because they lack the enzyme phytase. (illinois.edu)
Phytase is a feed enzyme fermented with an excellent genetic engineering strain and produced by advanced post treatment. (kitairu.net)

Current research suggests that β-propeller phytases are the major phytate-degrading enzymes in water and soil, and may play a major role in phytate-phosphorus cycling. (wikipedia.org)
citation needed] Only a few of the known phytases belong to a superfamily of enzymes called protein tyrosine phosphatases (PTPs). (wikipedia.org)
All characterized PTP-like phytases share an active site sequence motif (His-Cys-(X)5-Arg), a two-step, acid-base mechanism of dephosphorylation, and activity towards phosphrylated tyrosine residues, characteristics that are common to all PTP superfamily enzymes. (wikipedia.org)
With an estimated market value in excess of $350 million, phytase enzymes are used in 60 to 70 per cent of all monogastric feeds, generating a benefit to the animal feed industry worth $2 billion globally. (thefishsite.com)
Oluyinka AO, Cowieson AJ, Adeola O. Energy utilization and growth performance of broilers receiving diets supplemented with enzymes containing carbohydrase or phytase activity individually or in combination. (scielo.br)
To solve both of these problems, livestock feed is often supplemented with phytase enzymes, which break down the phytic acid in the animal's stomach. (rcsb.org)
Phytases are small, acid-stable enzymes that progressively break off the phosphate groups from phytic acid. (rcsb.org)
So, not all phytase enzymes are created equal and not all of them work the same way or have the same efficiency. (feedstrategy.com)
Microbial enzymes used as feed additives are mainly phytases and xylanases or beta-Glucanases. (fu-berlin.de)

Aspergillus (ficuum) niger fungal strain NRRL 3135 (ATCC 66876) was identified as a promising candidate as it was able to produce large amounts of extracellular phytases. (wikipedia.org)
The phytase from the fungus Aspergillus niger is a HAP and is well known for its high specific activity and its commercially marketed role as an animal feed additive to increase the bioavailability of phosphate from phytic acid in the grain-based diets of poultry and swine. (wikipedia.org)
The phytase gene was obtained from Aspergillus niger var. (cbd.int)
Root separation eliminated positive interactions in mixtures expressing the less mobile phytase (Aspergillus niger PhyA) whereas positive complementarity persisted in mixtures that expressed the more mobile phytase (Peniophora lycii PhyA). (elsevierpure.com)
The first group (control) was fed a basal diet with 4.6 g/kg available P. In contrast, the second, third, and fourth groups were fed diets treated with 3.2 g/kg available P, supplemented with either 5000 FTU/kg of bacterial E. coli (Quantum TM Blue 5G), fungal Aspergillus niger (VemoZyme ® F 5000 Naturally Thermostable Phytase (NTP)), or fungal Trichodermareesei (Yemzim ® FZ100). (olmdiagnostics.com)

The use of genetically modified corns and addition of microbial phytase are known to be effective methods for increasing nitrogen and phosphorus digestibility while reducing nutrient excretion. (porkgateway.org)
The objective of this study was to determine the nutrient digestibility of two genetically enhanced corns with the addition of microbial phytase when fed to finishing pigs. (porkgateway.org)
Catalytic and physicochemical properties of microbial phytase sources may differ, affecting phosphorus (P) release and subsequently the productive and reproductive performance of layers. (olmdiagnostics.com)
An experiment was conducted to determine how adding graded levels of microbial phytase affected the standardized total tract digestibility (STTD) of phosphorus in corn and corn co-products. (illinois.edu)
To each of these experimental diets was added microbial phytase to supply a total in the diet of 0, 500, 1,000, or 1,500 phytase units ( FTU ) per kg. (illinois.edu)
The amount of phosphorus, which is bound to phytate is significant in determining how the addition of microbial phytase will affect phosphorus digestibility. (illinois.edu)
Those ingredients with a relatively high ratio of phytate-bound phosphorus to total phosphorus are expected to show the greatest increase in phosphorus digestibility with the addition of microbial phytase. (illinois.edu)

This 1984 project led in 1991 to the first partially cloned phytase gene phyA (from A. niger NRRL 31235) and later on in 1993 to the cloning of the full gene and its overexpression in A. niger. (wikipedia.org)

Dr ten Doeschate explains that it is important to consider not just the amount of phosphorus a phytase can release - but whether sufficient phytate phosphorus exists in order to justify the use of a high dose of phytase with a phosphorus matrix. (abvista.com)

Several topics were explored using presentations and vibrant debates including nutrient digestibility, the analysis of phytate, phytase efficacy, differentiating between phytases and the future of phytase use in animal nutrition. (thefishsite.com)
Phytase individually or in combination with xylanase and protease improved the Ca and P digestibility of corn, soybean meal, and DDGS, from the hydrolysis of phytic acid. (animbiosci.org)
However, when xylanase was combined with phytase, it demonstrated a higher efficacy improving the nutrient digestibility of pigs. (animbiosci.org)

First, what is the amount of phosphorus each product releases per unit of phytase included in the final feed? (feedstrategy.com)

The effect of phytase increases nutrients and energy bioavailability for either the objective of this experiment was to evaluate the influence of nutrient and energy reduction in diets supplemented with phytase on the performance, gastrointestinal pH, organ and bone composition, and blood biochemistry of broilers between eight and 21 days of age. (scielo.br)
The present study was aimed to check the effect of phytase supplementation on muscle proximate composition in Labeo rohita fingerlings that are fed by phytase supplemented distiller's dried grain with soluble. (bigdatainagriculture.com)

Home News Company News Impact of increasing levels of phytase in diets with variable levels of. (zootecnicainternational.com)
Researchers from Texas A&M University, along with AB Vista Regional Technical Service Manager Craig Wyatt, have investigated the effect of increasing levels of phytase on broilers. (zootecnicainternational.com)
The objective of the study was to evaluate the influence of increasing levels of phytase with 3 levels of amino acids (AA) on broilers performance and breast yield. (zootecnicainternational.com)
The experiment was arranged as a 3 × 3 factorial ANOVA with of 3 levels of phytase at 500, 1,500, and 3,000 FTU/kg, and 3 AA densities. (zootecnicainternational.com)
From these data, regression equations were developed to predict the response to adding different levels of phytase to each ingredient. (illinois.edu)
The analyzed levels of phytase in the diets containing DDGS were 130, 430, 770, and 1,100 FTU/kg. (illinois.edu)

All members of this class of phytase share a common active site sequence motif (Arg-His-Gly-X-Arg-X-Pro) and have a two-step mechanism that hydrolyzes phytic acid (as well as some other phosphoesters). (wikipedia.org)
reported that dietary calcium levels higher than 0.70% at pH 6.0 promote the reaction of calcium with phytic acid, resulting in calcium phytate, which precipitates and cannot be broken down by phytase, consequently reducing phosphorus bioavailability. (scielo.br)
Phytase, with phytic acid in atomic colors. (rcsb.org)
Phytic acid (Wikim001 having high phytase activity. (biopaqc.com)
Phytase catalyzes the hydrolysis of phytic acid reducing its biding capacity and increasing bone and intestinal health, and the bioavailability of essential minerals and nutrients [ 17 - 19 ]. (animbiosci.org)

In 1999 Escherichia coli bacterial phytases were identified as being more effective than A. niger fungal phytases. (wikipedia.org)
Subsequently, this led to the animal feed use of this new generation of bacterial phytases which were superior to fungal phytases in many aspects. (wikipedia.org)

Our new video aims to give feed producers an insight into the key factors to consider when evaluating phosphorus equivalency, in order to determine the efficacy of a phytase. (abvista.com)

In 1991 BASF began to sell the first commercial phytase produced in A. niger under the trademark Natuphos which was used to increase the nutrient content of animal feed. (wikipedia.org)
What is of interest in the scope of this technical discussion is a very legitimate question that I have encountered, and answered, many times over: Are there real differences between the many commercial phytase products? (feedstrategy.com)

GERMANY - AB Vista will exhibit its revolutionary new phytase Quantum Blue on its stand (Hall 14, Stand J06) at the leading animal production trade show, EuroTier, from 11 to 14 November. (thepoultrysite.com)
Quantum Blue, which was launched to the European market earlier this year, is the most powerful and effective phytase on the market, and is currently delivering significant improvements in formulation costs, feed efficiency and return on investment for customers. (thepoultrysite.com)
So far in all markets where it has been introduced, Quantum Blue has established a significant market share, and has become the number one phytase in several of the global top 10 markets. (thepoultrysite.com)
This experiment, published in the Oxford Academic Journal of Applied Poultry Research, involved three levels of Quantum Blue phytase at 500, 1,500, and 3,000 FTU/kg, and three AA densities, for nine treatments across seven replicate pens with 45 Cobb 500 males per pen. (zootecnicainternational.com)

Work on phytases include characterization of new (bacterial, modified) phytases regarding their biochemical characteristics, stability in feed and in the digestive tract and this their suitability as feed additive. (fu-berlin.de)
The current study aimed to evaluate the impact of bacterial and fungal phytase sources on layer productivity, egg production, biochemical blood indices, and reproductive morphology. (olmdiagnostics.com)
Conclusively, bacterial and fungal phytase sources can compensate for the reduction of available P in layers' diets and enhance shell and yolk quality without affecting productive performance, and no differences among them were noticed. (olmdiagnostics.com)

Broiler fed the diet with reduced Ca and P levels and phytase supplementation presented the best performance of all groups. (scielo.br)
The dietary supplementation of the exogenous phytase reduces nutrient variability of feedstuffs, and counteracts the antinutritional effects of phytate, increasing the accuracy of feed formulation. (scielo.br)
Supplementation of phytase decreased the moisture and fat content of muscles in L. rohita fingerlings. (bigdatainagriculture.com)
Supplementation of phytase increases the crude protein and crude ash content of muscles in L. rohita fingerlings. (bigdatainagriculture.com)
The supplementation of protease was more effective when combined with phytase and xylanase in the soybean meal and DDGS possibly due to a higher protein content in these feedstuffs. (animbiosci.org)

This indicates that the value for STTD of phosphorus determined for DDGS without phytase may also be used if phytase is added to the diet at any inclusion level. (illinois.edu)

0.05) as phytase was added to the diets (Table 2). (illinois.edu)

The dietary inclusion of phytase increases nutrient and energy bioavailability for broilers. (scielo.br)
The bones of broilers fed phytase-supplemented diets presented higher ash content. (scielo.br)
Improving phosphorus availability in soybean meal for broilers by supplemental phytase. (scielo.br)
These data support the idea that "superdosing" phytase can improve broilers performance and breast meat yield. (zootecnicainternational.com)
Phytase breaks down phytate, releasing phosphorus and other chelated minerals for use by broilers. (zootecnicainternational.com)
Performance, Bone Parameters and Phosphorus Excretion of Broilers Fed Low Phosphorus Diets Supplemented with Phytase from 23 to 40 Days of Age. (cu.edu.eg)
El-Sherbiny, A. E., H. M. A. Hassan, M. O. Abd-Elsamee, A. Samy, and M. A. Mohamed, 'Performance, Bone Parameters and Phosphorus Excretion of Broilers Fed Low Phosphorus Diets Supplemented with Phytase from 23 to 40 Days of Age. (cu.edu.eg)

Phytase activity is optimal at pH between 5.0 and 6.5, and it is reduced when pH is lower or equal to 3.0 ( Casey & Walsh, 2004 Casey A, Walsh G. Identification and characterization of a phytase of potential commercial interest. (scielo.br)

Our range of phytases increases natural phosphorus availability, improving performance while reducing feed costs and environmental impact. (dsm.com)

It is widely thought that phytase can be supplemented into poultry diets to increase utilisation of amino acids. (zootecnicainternational.com)
Six experimental diets were prepared by supplementing phytase at graded levels. (bigdatainagriculture.com)

Four distinct classes of phytase have been characterized in the literature: histidine acid phosphatases (HAPS), beta-propeller phytases (BPPs), purple acid phosphatases (PAPs), and most recently, protein tyrosine phosphatase-like phytases (PTP-like phytases). (wikipedia.org)
Histidine acid phytases (HAPhy), like the phytases used as feed supplements, have a histidine to perform the reaction. (rcsb.org)
The phytase AppA from Escherichia coli uses a histidine in its reaction (magenta), which is activated by a neighboring aspartate (pink). (rcsb.org)

A high phytase activity in food and feedstuffs is desirable to ensure high bioavailability of phytate bound phosphate and counter the antinutritional effects of non-digested phytate. (frontiersin.org)

Mature grain phytase activity (MGPA) in the Triticea tribe cereals has evolved through gene duplications and neo-functionalization of the purple acid phosphatase phytase gene ( PAPhy ) in a common ancestor. (frontiersin.org)
Purple acid phytases (PAPhy, PDB entry 4kbp ) contain metal ions and have a purple color. (rcsb.org)

The nutritional components and protein quality of genetically modified maize expressing phytase gene (GM) were analyzed and evaluated in this study. (croplife.org)

HAPs have also been overexpressed in several transgenic plants as a potential alternative method of phytase production for the animal feed industry and very recently, the HAP phytase gene from E. coli has been successfully expressed in a transgenic pig. (wikipedia.org)
FinaseEC is an E. coli-derived 6-phytase developed for use in swine and poultry feeds. (abvista.com)

However, barley only contains one HvPAPhy_a gene and the MGPA levels needs to be increased to substitute for the addition of microbial phytases to the feed. (frontiersin.org)
Furthermore, the gene dose response of phytase cisgenes from three different cisgenic barley plants were horizontally stacked. (frontiersin.org)

Phytases (myo-inositol hexakisphosphate 3-,6- and 5-phosphohydrolase, EC 3.1.3.8, EC 3.1.3.26, and EC 3.1.3.72) are phosphatases that can initiate the stepwise hydrolysis of phytate, the main storage form of phosphate in seeds and the major antinutritional factor for bio-availability of micronutrient in food and feed ( Lott, 1984 ). (frontiersin.org)

A phytase has recently been isolated from the cotyledons of germinating soybeans that has the active site motif of a purple acid phosphatase (PAP). (wikipedia.org)
The diet with reduced amino acid and energy levels and phytase addition reduced gizzard and proventriculus pH. (scielo.br)

PTPhy phytases, such as the one in PDB entry 3mmj , are similar to protein tyrosine phosphatases. (rcsb.org)

Beta-propeller phytases (BPPhy, PDB entry 3amr ) are named for their unusual protein fold. (rcsb.org)
In recent years," says Dr ten Doeschate,"the functionality of phytases has evolved - and there is greater awareness of their ability to release additional minerals and protein beyond phosphorus. (abvista.com)

The selected 22 isolates were further evaluated among them, 4 isolates PR2, PR6, PR16, PR24 could be grown on PSM plate with 2.5% agar after incubation of 24 hr at 37°C temperature and shown phytase activity in shake flask experiment. (journalcra.com)
For the purposes of this experiment, analyzed values for phytase activity were used in all calculations instead of calculated values. (illinois.edu)

While phytases have been found to occur in animals, plants, fungi and bacteria, phytases have been most commonly detected and characterized from fungi. (wikipedia.org)
PTP-like phytases, a relatively newly discovered class of phytase, have been isolated from bacteria that normally inhabit the gut of ruminant animals. (wikipedia.org)
The main objectives of the research were to isolate and characterize phytase producing bacteria from traditional fermented foods like idli and jalebi batter, and optimize phytase production by the isolated strain of bacteria. (journalcra.com)

UT PHOS degrades phytase phosphrous existing in vegetable ingredients of feed for poultry & livestock, releases phosphrous & other nutritional substances. (venkys.com)

High dietary calcium levels change the pH of the upper sections of the digestive tract, inactivating phytase. (scielo.br)

However, only the PAP from soybeans has been found to have any significant phytase activity. (wikipedia.org)
Our prior study uncovered that Wikim001 isolated from kimchi provides high phytase activity and is effective for reducing the phytate items in brown grain (17). (biopaqc.com)
Phytate is not hydrolyzed by fish because natural phytase activity not present in some agastric fishes, phytate is present in grain products as the main form of phosphorus. (bigdatainagriculture.com)

Say, if you add 1 kilogram of phytase A product as recommended by the manufacturer (note, here I am not referring to active compound inclusion, but rather on final product inclusion), do you receive back the 0.1% available or digestible phosphorus, as claimed by the manufacturer? (feedstrategy.com)
All quality phytases must deliver the phosphorus they promise regardless of their origin, name, mode of action or inclusion rate. (feedstrategy.com)

In conclusion, phytase showed randomized responses to muscle composition. (bigdatainagriculture.com)

In 1962 began the first attempt at commercializing phytases for animal feed nutrition enhancing purposes when International Minerals & Chemicals (IMC) studied over 2000 microorganisms to find the most suitable ones for phytase production. (wikipedia.org)

Anatomy1

Organisms4

Diseases1

Chemicals and Drugs5

Analytical, Diagnostic and Therapeutic Techniques and Equipment1

Phenomena and Processes6

Technology, Industry, Agriculture4

Health Care1

Enzyme13

Enzymes9

Aspergillus5

Microbial phytase7

Cloned phytase gene1

Phytate-phosphorus1

Digestibility3

Unit of phytase1

Effect of phytase2

Levels of phytase6

Phytic acid5

Fungal phytases2

Efficacy1

Commercial phytase2

Quantum Blue4

Bacterial3

Supplementation5

DDGS1

0.051

Broilers7

Characterization1

Increases1

Diets2

Histidine3

Feedstuffs1

PAPhy2

Genetically modified1

Coli2

Gene2

Hexakisphosphate1

Acid2

Phosphatases1

Protein2

Experiment2

Bacteria3

Ingredients1

Digestive tract1

Activity3

Inclusion2

Conclusion1

20001