Loading...
  • vivo
  • The long term goal of this work is to establish whether chemicals which are identified on the basis of in vitro bioactivity are predictive of effects in vivo and to determine which endocrine responses are most sensitive in terms of whole animal and population levels effects. (comparativephys.ca)
  • Phosphofructokinase activity as a measure of maturation of rat oocytes developed in vivo and in vitro. (nii.ac.jp)
  • Hexokinase activity in mouse embryo developed in vivo and in vitro. (nii.ac.jp)
  • Current work focuses on utilising state-of-the-art proteomic techniques and novel in vivo cell imaging systems to investigate the interaction between ram spermatozoa, seminal plasma and the ewe's reproductive tract. (edu.au)
  • Although embryos resulting from IVF were less successful in establishing pregnancy than appropriate in vivo fertilized controls, optimization of culture and transfer techniques enabled minimization of embyronic losses and permitted valid use of these techniques. (uwo.ca)
  • We used piggyBac to gene-modify cytotoxic T lymphocytes to carry a chimeric antigen receptor directed against the tumor antigen HER2, and found that gene-modified T cells mediated targeted killing of HER2-positive tumor cells in vitro and in vivo in an orthotopic mouse model 14 . (jove.com)
  • embryo production
  • I was awarded a scholarship in 1988 by the Nordic Ministry Council and spent 3 months studying bovine embryo production in vitro and embryo transfer at the Royal Veterinary and Agricultural University in Copenhagen, Denmark. (ibream.org)
  • In vitro embryo production efficiency in cattle and its association with oocyte adenosine triphosphate content, quantity of mitochondrial DNA, and mitochondrial DNA haplogroup. (springermedizin.de)
  • ovary
  • In humans, oocytes are established in the ovary before birth and may lie dormant awaiting initiation for up to 50 years. (wikipedia.org)
  • In addition, we are addressing the relevance of growth factors to ovarian physiology (IGFs, steroids, retinoids) by studying their actions and assessing of their sites of synthesis within the ovary and regulation of their production. (comparativephys.ca)
  • Oocytes are manually defolliculated from donor ovary tissue, injected or treated in culture as desired, and then stimulated with progesterone to induce maturation. (jove.com)
  • Although the effects of Gonadotropin on ovarian physiology have been known for many decades, its action on glucose uptake in the rat ovary remained poorly understood. (jove.com)
  • preovulatory
  • Mature oocyte collection from the preovulatory follicles in normal cycle women only became possible after the introduction of laparoscopy into gynecology practice in the 1970s (10) . (tjoddergisi.org)
  • factors to regulate
  • However, it is unclear how gonadotropins modulate these factors to regulate oocyte maturation, especially by gap junctional communication (GJC). (jove.com)
  • The recognition of oocyte maturation arrest as a specific medical condition may contribute to the characterization of the yet poorly defined entity currently known as 'oocyte factor' (oocyte secretes its own factors to regulate microenvironment in close neighbourhood of surrounding cells). (fertilitypedia.org)
  • Xenopus
  • Additionally, many of the techniques to overexpress or inhibit gene function in organisms such as Xenopus and zebrafish fail to sufficiently target critical maternal signaling pathways, such as Wnt signaling. (jove.com)
  • In Xenopus , manipulating gene function in cultured oocytes and subsequently fertilizing them can ameliorate these problems to some extent. (jove.com)
  • Electrophysiological studies that employ Xenopus oocytes include: 1) analysis of channel permeability properties and pharmacological sensitivity, 2) investigation of the properties of mutant channel subunits to address structure-function issues, 3) analysis of channel post-translational modifications, assembly and stoichiometry, 4) study of channel modulation by receptors and second messengers, 5) functional screens for genes encoding ion channels and transporters (expression-cloning). (wormbook.org)
  • The most common approach for expression of exogenous proteins in Xenopus oocytes is to inject in vitro transcribed complementary RNA into the oocyte cytoplasm ( Figure 1a ). (wormbook.org)
  • (a) Cartoon representing the process of expressing exogenous proteins in Xenopus oocytes. (wormbook.org)
  • sperm physiology
  • This research project intends to further the understanding of dromedary camel sperm physiology so as to determine methods to improve its quality and facilitate effective artificial insemination following liquid (and possibly frozen) storage. (edu.au)
  • intracytoplasmic sperm inj
  • Interventions include intrauterine insemination plus controlled ovarian stimulation, in vitro fertilisation, intracytoplasmic sperm injection, gonadotrophin releasing hormone agonists, clomifene and tamoxifen, laparoscopic ovarian drilling, tubal flushing and laparoscopic ablation of endometrial deposits, depending on the cause of the infertility (Al-Inany 2004). (acupuncture.org.uk)
  • fertilisation
  • Most clinical trials to date suggest that acupuncture may be useful in the embryo transfer stage of in vitro fertilisation, and results in an increased pregnancy rate and a greater number of live births (Cheong 2008, Manheimer 2008, Kong 2009, Chen 2009, Smith 2006, Westergaard 2006), though there have been exceptions (Domar 2009) (see Table overleaf). (acupuncture.org.uk)
  • increasing egg production (Jin 2009) and improving oocyte quality (Chen 2009), which could increase the chance of fertilisation. (acupuncture.org.uk)
  • Microinjection
  • The method that will be reviewed in this chapter is the one based on microinjection of complementary RNA (cRNA) into the oocyte cytoplasm ( Figure 1a ). (wormbook.org)
  • germ cell
  • Co-expression of cyp26a1 and the pluripotent germ cell stem cell marker pou5f1(oct4) in meiotically arrested oocytes was consistent with roles in mouse to promote germ cell survival and to prevent apoptosis, mechanisms that are central for tipping the sexual fate of gonads towards the female pathway in zebrafish. (jove.com)
  • embryology
  • He was the first to extensively study the embryology and physiology of reproduction in opossums when little was known about this mammal. (asu.edu)
  • Metabolism
  • Collectively, our results showed that resveratrol can protect the oocytes from methylglyoxal-induced cytotoxicity and this was mainly through the correction of the abnormity of cellular reactive oxygen species metabolism. (jove.com)
  • regulates critical processes in muscle physiology, including mitochondrial biogenesis, lipid metabolism and angiogenesis. (jove.com)
  • differentiation
  • Molecular methods and immunoassay can evaluate stage of maturation and guide adequate differentiation. (wikipedia.org)
  • Together, our data suggest that palladin might have both positive and negative roles in maintaining the proper skeletal myogenic differentiation in vitro. (jove.com)
  • inhibition
  • In this study, we showed that resveratrol, a kind of phytoalexin found in the skin of grapes, red wine and other botanical extracts, can alleviate the adverse effects caused by methylglyoxal, such as inhibition of oocyte maturation and disruption of spindle assembly. (jove.com)
  • Dual roles of palladin protein in in vitro myogenesis: inhibition of early induction but promotion of myotube maturation. (jove.com)
  • clinical
  • quantitative aspects of physiology and engineering applications to clinical medicine. (tamu.edu)
  • Throughout these industries, there are no strict guidelines as to the in vitro handling methods for these procedures, leading to the question of whether different in vitro conditions influence the outcomes of semen analyses and ultimately if this impacts upon clinical decision making. (edu.au)
  • females
  • Previous studies showed that the accumulation of methylglyoxal and advanced glycation ends can impair the oocyte maturation and reduce the oocyte quality in aged and diabetic females. (jove.com)
  • induce
  • In the presence of the GJC inhibitor carbenoxolone (CBX), FSH failed to induce the meiotic resumption and the changes in PKAI, GPR3 and cAMP surge in oocyte were no longer detected. (jove.com)