Physiological analysis of genes and proteins. Medical search. Frequent questions

Recent molecular analyses of mt genome sequences have provided selectable hypotheses for the phylogenetic relationships of nematodes based on 18S rRNA. (nature.com)
While progress has been made on understanding the etiology of ZC, there is limited information on host-specific molecular and physiological responses associated with the disease. (usda.gov)
Conclusions: In summary, this genome-wide identification, characterization and expression analysis of legume bZIP genes provides valuable information for understanding the molecular functions and evolution of the legume bZIP transcription factor family, and highlights potential legume bZIP genes involved in regulating tissue development and abiotic stress responses. (datadryad.org)
Since these profiles map the physiological state of the respective organ on a molecular level, the proteome profile of an organ changes in the course of the development and progression of a disease, even before changes in organ function can be medically detected. (unimedizin-mainz.de)
Thymic epithelial cells (TEC) avert autoimmunity through their ability to promiscuously express virtually the entire protein-coding gene repertoire as a molecular library against which immature T cells are selected. (sanger.ac.uk)
An integrative analysis of the transcriptome, epigenome and proteome of distinct TEC subpopulations will be used to attain an unparalleled systems-level understanding of the molecular conditions that select a tolerant T cell repertoire under normal physiological conditions. (sanger.ac.uk)
Membrane trafficking defects caused by mutation in OCRL may explain renal tubular defects observed in Lowe syndrome, including the inability of proximal tubular cells (PTC) to reabsorb low-molecular weight (LMW) proteins and other solutes such as phosphorus and bicarbonate from the glomerular filtrate. (medscape.com)
The low number of megalin at the PTC apical border explains the reduced endocytosis of low-molecular weight proteins that occur in Lowe syndrome. (medscape.com)
Composed of 2 major components, the C. difficile S-layer has high and low molecular weight proteins (HMW and LMW, respectively), which are formed from the posttranslational cleavage of a single precursor, surface layer protein A (slpA) ( 6 ). (cdc.gov)
These encompass cutting-edge techniques such as advanced flow cytometry, confocal microscopy, molecular methodologies, including CRISPR/Cas-9 gene editing, investigations into protein interactions, and specialized assays tailored for β-cell research. (lu.se)
The main aim of our research is to understand the molecular details and physiological roles of the multitude of water channel-like proteins present in plants and animals. (lu.se)

Trehalase plays a significant role in various physiological processes in insects. (arizona.edu)
As bioactive compounds tend to interact with multiple proteins with various physiological effect, we aimed to identify other potential shared targets to understand the extent in which these chemicals could lead to GWI. (cdc.gov)

We examined the extent to which [transcriptional co-expression] is effective for our data, and we show that it yields almost universally superior predictions of gene function in comparison to using information regarding simple tissue specificity or tissue restriction" say the authors. (sciencedaily.com)
The results of the study also hint at a more complex transcriptional control in mammals, whereby transcription factors may be regulating the transcription of functionally related genes across different tissues. (sciencedaily.com)
They have been shown to be diverse transcriptional regulators, playing crucial roles in plant development, physiological processes, and biotic/abiotic stress responses. (datadryad.org)
see RTKRas/MAP kinase signaling ), LIN-12 (see LIN-12/Notch signaling in C. elegans ), and WNT (see Wnt signaling ), as well as the functions of the SynMuv and mediator proteins (see Transcriptional regulation ). (wormbook.org)
The exon junction complex (EJC) deposited on spliced mRNAs, plays a central role in the post-transcriptional gene regulation and specific gene expression. (nature.com)
The Exon Junction Complex (EJC) plays a central role in post-transcriptional gene expression control. (nature.com)
Using these animal models a large body of evidence has implicated PARP-1 in the regulation of DNA repair and transcriptional activity of eukaryotic genes. (imim.es)
New CodY independent transcriptional changes were detected including genes involved in the SOS response, iron storage (e.g. ftnA, dps), oxidative stress response (e.g., perR, katA, sodA) and the psmα1-4 and psmß1-2 operons coding for cytotoxic, phenol soluble modulins (PSMs). (nih.gov)
Secondary, mRNA-seq analysis identified Gadd45 as a potential transcriptional target of dGLYAT, as depletion of dGLYAT decreased Gadd45 mRNA level. (sdbonline.org)
Typical applications include single-cell measurements of gene expression, analysis of transcriptional reporters, and cell cycle studies in prokaryotic and eukaryotic microorganisms. (uni-marburg.de)

Under normal physiological conditions only those proteins of the entire proteome are expressed that are necessary for the execution of organ functions at a given time - thus resulting in organ-specific proteome profiles. (unimedizin-mainz.de)

Putative large exoproteins and a putative protein with a RTX toxin-type ß-roll could be identified. (dsmz.de)
This putative gene has been named gau for g ene a ntisense u biquitous in mtDNAs. (biomedcentral.com)
In view to find putative mitochondrial genes in alternative reading frames on both coding and "presupposed" non-coding strands, numerous complete mtDNA genomes have been analyzed. (biomedcentral.com)
Sequence alignment and phylogenetic analysis of the two putative proteins revealed that HaTreh-1 and HaTreh-2 belong to soluble and membrane-bound trehalase groups, respectively. (arizona.edu)
Micro- array data from the ovalbumin and TMA model of asthma were also compared to previous data using Aspergillus as allergen to identify putative asthma `signature genes', i.e. genes up-regulated with all 3 allergens. (cdc.gov)

After a long period of evolution, the genetic composition of mt in most metazoans is now present consistently, suggesting that mt genes are important for maintaining the basic functions of mt 9 . (nature.com)
In November 2019, Maze Therapeutics announced that their intention to find out a way to manipulate proteins in genetic code is now giving way to a new system called CRISPR gene editing. (medgadget.com)
Moreover, this strengthens evidence for mitochondrial genes coded according to an overlapping genetic code. (biomedcentral.com)
An ectotherm's thermal tolerance is determined by both fixed genetic factors and plastic changes in behavior, morphology, physiology, and gene expression. (frontiersin.org)
Other mutations insert or delete genetic material in the GLDC gene, or disrupt how genetic information from the gene is spliced together to make a blueprint for producing glycine dehydrogenase. (medlineplus.gov)
Conter C, Rolland MO, Cheillan D, Bonnet V, Maire I, Froissart R. Genetic heterogeneity of the GLDC gene in 28 unrelated patients with glycine encephalopathy. (medlineplus.gov)
As a result, we now know many genetic defects responsible for neurological disease, but frequently we do not know much about the resulting protein product and therefore the pathophysiologic basis for the disease. (medscape.com)

The growth and development of neovascular tissue or angiogenesis are critical for normal physiological processes. (hindawi.com)
Ethanol can interfere with cell membrane's ability to act as a barrier, and interrupt key cellular processes such as protein biosynthesis, energy transduction and transport[ 8 ]. (biomedcentral.com)
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue. (lookformedical.com)
Here, using the model species Drosophila melanogaster , we used a combination of Genome Wide Association mapping (GWAS) and transcriptomic profiling to characterize whether genes associated with thermal tolerance are primarily involved in dynamic stress responses or preparatory processes that influence physiological condition at the time of thermal stress. (frontiersin.org)
We observed greater variation in lower thermal limits, with CT min ranging from 1.81 to 8.60°C, while CT max ranged from 38.74 to 40.64°C. We identified 151 and 99 distinct genes associated with CT min and CT max , respectively, and there was strong support that these genes are involved in both dynamic responses to thermal stress and preparatory processes that increase thermal resistance. (frontiersin.org)
Our research group specializes in the study of innate immunity, with a particular emphasis on its role in both physiological and pathological processes, including diabetes. (lu.se)

This will not only improve our understanding of the physiological basis of interactions in cyanobacterial co-cultures, but also will add value to the cyanobacteria collection and guide subsequent attempts to obtain pure (axenic) cultures. (dsmz.de)
Intestinal mucins trigger immune responses upon recognition by dendritic cells via protein-carbohydrate interactions. (bvsalud.org)
Imaging-based fluorescence experiments, on the other hand, allow us to get more insights into living cells, e.g., by measuring protein properties such as interactions or diffusion. (uni-marburg.de)
As the design and function of signaling networks depends on protein-protein interactions, which in turn are affected by protein diffusion, resolving such properties in space and time helps to understand signaling up to system-level properties such as signal integration and amplification. (uni-marburg.de)
Protein-protein interactions in living cells are monitored in a quantitative time- and space-resolved fashion by the microscopy-based Förster Resonance Energy Transfer (FRET) approach. (uni-marburg.de)

Moreover, transcriptomic analyses have demonstrated that, in free-living bacteria, gene transcription levels and chromosomal organization are mutually influenced. (biomedcentral.com)
Using an oligonucleotide-based microarray, we normalized the transcriptomic data by genomic DNA signals in order to have access to inter-gene comparison data. (biomedcentral.com)
RNA-Seq based global transcriptomic analysis provided a comprehensive view of cellular response to ethanol exposure. (biomedcentral.com)
These results also have utility for directly comparing GWAS and transcriptomic approaches for identifying candidate genes associated with thermal tolerance. (frontiersin.org)

Interestingly, in spite of an Lso-mediated down-regulation of photosynthesis-related genes/proteins, over 80% of the differentially produced gene transcripts/proteins were up-regulated in AG tissues. (usda.gov)
In general, results suggest that ZC disease development involves an Lso-mediated down-regulation of photosynthesis accompanied by an ineffective and potentially inefficient up-regulation of stress response-, metabolism-, and housekeeping-related gene transcripts/proteins with concomitant increases in nutrient accumulation. (usda.gov)
This study provides the first evidence that different EJCs can have distinct functions, illuminating EJC-dependent gene regulation. (nature.com)
Gene organization in the bacterial chromosome has very well known effects on transcription and a standard example is the organization of genes into operons, which allows for a sophisticated regulation of gene expression [ 9 ]. (biomedcentral.com)
Analyses of the ftnA, dps and psm genes in different regulatory mutants revealed that their (pp)pGpp-dependent regulation can occur independent of the regulators PerR, Fur, SarA or CodY. (nih.gov)
Further, several GWAS candidates were regulatory genes that may participate in the regulation of stress responses, and gene ontologies related to development and morphogenesis were enriched, suggesting many of these genes influence thermal tolerance through effects on development and physiological status. (frontiersin.org)
Continued studies focused on genes showing minimal changes in the TMA-induced model but with clear up-regulation in the ovalbumin model. (cdc.gov)

The early environment can have short- and long-term impacts on the physiology of many animal species and can be used to influence physiological responses that is called programming. (biomedcentral.com)

Phylogenetic analysis also showed that W. compar and W. siamensis formed a sister group. (nature.com)
To do so, the researchers first performed a phylogenetic analysis of the TPC genes in the green plant lineage. (sciencedaily.com)

MetaNetwork Enhances Biological Insights from Quantitative Proteomics Differences by Combining Clustering and Enrichment Analyses. (stanford.edu)
Interpreting proteomics data remains challenging due to the large number of proteins that are quantified by modern mass spectrometry methods. (stanford.edu)
The protein analysis was performed by using the SignalP 3.0 server ( www.cbs.dtu.dk/services/SignalP/ ) and the ExPASy Proteomics server ( www.expasy.ch/tools/pi_tool.html ). (cdc.gov)

The human cellular prion protein (PrP C ) is a glycosylphosphatidylinositol (GPI) anchored membrane glycoprotein with two N-glycosylation sites at residues 181 and 197. (mdpi.com)
MS membrane proteins are essential for tissue development, cellular motion, osmotic homeostasis, and sensing external and self-generated mechanical cues like those responsible for touch and proprioception. (stanford.edu)
A substantial number of proteins that are involved in the heat-acclimatization process such as the proteins related to cellular redox status and heat shock, are already altered in the type-A arr mutants without a prior exposure to stress conditions. (frontiersin.org)
These observations strongly suggest the implication of EJC in fine-tuning of specific gene expression in different cellular contexts. (nature.com)
Since liver sampling was only possible from the 12th day of embryogenesis (E12), we analyzed hepatic gene expression at 7 sampling points every 4 days from this point up to 4 days post-hatch (D4) and revealed different patterns of expression depending on the cellular pathway. (biomedcentral.com)
For a more thorough review of the role of phosphatidylinositol and the cellular and physiological functions of OCRL1 please refer to the following 2 reviews: (1) McCrea HJ, De Camilli P. Mutations in phosphoinositide metabolizing enzymes and human disease. (medscape.com)
[ 2 ] and (2) Mehta ZB, Pietka G, Lowe M. The cellular and physiological functions of the Lowe syndrome protein OCRL1. (medscape.com)

The chromosome distribution and analysis for WGD-derived duplicated blocks revealed that the legume bZIP genes have expanded mainly by segmental duplication rather than tandem duplication. (datadryad.org)
Past genomic studies have comprehensively described the organization of the bacterial chromosome, for example in terms of gene localization, order and orientation. (biomedcentral.com)
It has also been shown that neighbouring genes in a bacterial chromosome tend to be co-expressed, even if they are not in the same operon [ 10 ], suggesting the existence of a "supra-operonic" organization [ 11 ]. (biomedcentral.com)
Lowe syndrome is caused by an inherited mutation in the OCRL gene, mapped to chromosome Xq 26.1, which encodes the OCRL1 protein. (medscape.com)

To do this, we use a combination of quantitative behavioral analysis, genetics, in vivo electrophysiology, and heterologous expression of ion channels. (stanford.edu)

We sequenced the complete mt genome of W. compar and conducted phylogenetic analyses using concatenated coding sequences of 12 protein-coding genes (PCGs) by maximum likelihood and Bayesian inference. (nature.com)
This file includes the bZIPs protein sequences from Arabidopsis thaliana and six legume genomes (Glycine max,Medicago truncatula,Cajanus cajan,Cicer arietinum,Phaseolus vulgaris and Lotus japonicus). (datadryad.org)
Human biology is tightly linked to proteins, yet most measurements do not precisely determine alternatively spliced sequences or posttranslational modifications. (stanford.edu)
Only when hybridisation is complete, protein-specific DNA reporter sequences are formed by enzymatic extension of the oligonucleotides, which are detected by amplification using qPCR. (unimedizin-mainz.de)

A pancancer analysis was performed on NRP1 and NRP2 mRNA expression levels in the Oncomine database ( http://www.ONCOMINE.org ). (hindawi.com)
The potential anti-neuroinflammatory effects of propranolol were interrogated by analysis of cytokine mRNA expression. (cdc.gov)

The mutants of multiple type-A ARR genes exhibit improved basal and acquired thermotolerance and, altered response to oxidative stress in our physiological analyses. (frontiersin.org)
The metabolomics analyses further reveal that the mutants accumulate higher amounts of α-and γ-tocopherols, which are important antioxidants for protection against oxidative damage. (frontiersin.org)
Unfavorably high temperature causes heat stress in all organisms and leads to disintegration of membrane lipids, protein misfolding and denaturation, oxidative burst, and increased membrane fluidity. (frontiersin.org)
The expression of central genes of the oxidative stress response protects the bacteria from anticipated ROS stress derived from PSMs or exogenous sources. (nih.gov)
Furthermore, GSOs protected cells against GLU-induced apoptosis by reducing the expression of the mitochondrial apoptosis-associated Bcl-2 family effector proteins and protected cells from GLU-induced oxidative damage by increasing the nuclear translocation of Nrf2 and HO-1 expression. (sdbonline.org)
Typical triploblastic animal mtDNAs contain genes that encode the large and small subunit ribosomal RNAs, 22 transfer RNAs (tRNAs), and 13 proteins that are all components of the oxidative phosphorylation process: ATPase subunits 6 and 8 (ATP6 and 8), cytochrome b (Cyt b), cytochrome c oxidase subunits 1-3 (Cox1-3) and NADH dehydrogenase subunits 1-6 and 4L (ND1-6 and 4L). (biomedcentral.com)

In this study, RNA-Seq, 2-dimension gel glectrophoresis , mass spectrometry and qPCR analyses identified over 100 differentially produced gene transcripts and protein spots in above-ground (AG) and below-ground (BG) potato tissues upon Lso infection. (usda.gov)

We also detected several key legume bZIP genes involved in drought- and salt-responses by comparing fold changes of expression values in drought-stressed or salt-stressed roots and leaves. (datadryad.org)
To build a foundation necessary to engineer robust ethanol-producing cyanobacterial hosts, in this study we applied a quantitative transcriptomics approach with a next-generation sequencing technology, combined with quantitative reverse-transcript PCR (RT-PCR) analysis, to reveal the global metabolic responses to ethanol in model cyanobacterial Synechocystis sp. (biomedcentral.com)
The results showed that ethanol exposure induced genes involved in common stress responses, transporting and cell envelope modification. (biomedcentral.com)
Indeed embryogenesis is a period of development with high plasticity which can be disturbed by environmental stimuli leading to a modification of certain physiological responses in adulthood [ 1 , 2 ]. (biomedcentral.com)
in many higher vascular plants such as Arabidopsis thaliana (Arabidopsis) and Oryza sativa (rice), a single TPC gene is involved in the activity of slow vacuolar (SV) channels (voltage-dependent cation channels) along with long-distance signalling, defence, and responses to environmental stress. (sciencedaily.com)
For example, previously we found that several GWI-relevant organophosphate acetylcholinesterase inhibitors produce heightened neuroinflammatory responses following subchronic exposure to stress hormone as a mimic of high physiological stress. (cdc.gov)

Indeed, bacterial genomes carry, on average, from 78% (for genomes containing the polymerase PolC) to 58% (for the other genomes) of their genes on the leading strand [ 3 ]. (biomedcentral.com)
With roughly 30,000 genes in mammalian genomes, fection with a vector encoding MyoD (Tapscott et al. (lu.se)

Jan. 13, 2023 When genes are activated and expressed, they show patterns in cells that are similar in type and function across tissues and organs. (sciencedaily.com)
Therefore, we analyzed a series of glycoproteomes in several mouse tissues to identify glycosylated proteins and their glycosylation sites. (nih.gov)
Expression data further revealed that the legume bZIP genes were expressed constitutively or in an organ-specific, development-dependent manner playing roles in multiple seed developmental stages and tissues. (datadryad.org)
However, analysis of residual basal poly(ADP-ribosyl)ation in PARP-1-/- tissues have demonstrated a high percentage of PARP activity in thymus (31% activity) and spleen (82%activity). (imim.es)

A multidimensional bioinformatics analysis was performed to examine the relationship between NRP genes and prognostic and pathological features, tumour mutational burden (TMB), microsatellite instability (MSI), and immunological features based on public databases and find the potential prognostic value of NRPs in pancancer. (hindawi.com)

Symbiosis genes" of the epibiont were identified by genomic approaches. (dsmz.de)
Sincle cell genomic analysis of the bacteria constituting different types of phototrophic consortia indicate a long-term co-evolution of the partners. (dsmz.de)
Genomic studies on bacteria have clearly shown the existence of chromosomal organization as regards, for example, to gene localization, order and orientation. (biomedcentral.com)
However in recent years with the development of epigenesis, genomic imprinting and the horizontal transferences of the genes, Lamarck's ideas have resurfaced. (intechopen.com)
Analysis of human GRF complementary DNA 10,11 and genomic 12 clones indicates that the GRF peptides are first synthesized as a 107- or 108-amino-acid precursor protein. (northwestern.edu)

Most genome-wide analyses have been performed under stress conditions, which often mask the immediate effects of (pp)pGpp-mediated regulatory circuits. (nih.gov)
Therefore, the aim of the present study was to analyze a wide range of genes involved in liver development, cell stress, lipid and carbohydrate metabolisms throughout embryogenesis in mule ducks to better understand the ontogeny of pathways related to liver fattening. (biomedcentral.com)
MAIN METHODS: Adult male C57BL/6J mice received a subchronic exposure to corticosterone (CORT) at levels mimicking high physiological stress followed by exposure to the sarin surrogate, diisopropyl fluorophosphate (DFP). (cdc.gov)

Finally, we used gene knockout strains to validate the potential target genes related to ethanol tolerance. (biomedcentral.com)

Mechanosensitive membrane proteins: Usual and unusual suspects in mediating mechanotransduction. (stanford.edu)
This Viewpoint, which accompanies a Special Issue focusing on membrane mechanosensors, discusses unifying and unique features of both established and emerging mechanosensitive (MS) membrane proteins, their distribution across protein families and phyla, and current and future challenges in the study of these important proteins and their partners. (stanford.edu)
As the field continues to grow, so too will the list of proteins suspected to function as mechanosensors and the diversity of known MS membrane proteins. (stanford.edu)
In this study, we cloned and characterized a soluble trehalase gene (HaTreh-1) and a membrane-bound trehalase gene (HaTreh-2) from Helicoverpa armigera, a serious polyphagous pest of crops. (arizona.edu)
The researchers also found that the three TPC proteins were primarily localized at the vacuolar membrane of M. polymorpha. (sciencedaily.com)
Affinity tags can reduce merohedral twinning of membrane protein crystals. (lu.se)

The proteome research laboratory is equipped with a high-throughput analysis platform based on immuno-qPCR technology. (unimedizin-mainz.de)
The extraordinarily high specificity of immuno-qPCR-based protein determinations results from the need for spatial proximity binding of 2 different antibodies to the same molecule of a target protein ® Olink Proximity Extension Assay (PEA) technology (Figure 2). (unimedizin-mainz.de)
Quantitative real-time PCR (qPCR) analyses of the spatiotemporal expression pattern of HaTreh in H. armigera revealed that HaTreh-1 was expressed mainly in the midgut, with lower expression in the integument and head, Malpighian tubules, trachea, and fat body. (arizona.edu)

Different statistical methods with varying selection criteria resulted in identification of similar candidate genes. (cdc.gov)
Array data are intended to provide candidate genes for hypothesis generation and further experimentation. (cdc.gov)
Array studies provide candidate genes to identify common mechanistic pathways in the effector phase, as well as mechanistic pathways unique to individual allergens. (cdc.gov)

Berry intake changes hepatic gene expression and DNA methylation patterns associated with high-fat diet. (lu.se)

Protein diffusion plays a crucial role in determining what function a protein serves within the cell and how, when and where it may physically interact with other proteins and macromolecules in response to external stimuli. (uni-marburg.de)

Genome-wide association analysis revealed that the NRP1 expression was strongly associated with kidney renal clear cell carcinoma (KIRC), whereas the NRP2 expression was closely associated with BLCA. (hindawi.com)

It has been assumed that this strategy couldn't be applied to mammals, but instead that genes expressed in the same tissue are most likely to have a functional relationship, making tissue-specificity the best indicator of function. (sciencedaily.com)
This new, quick, high-throughput method for predicting mammalian gene function merely from the pattern of RNA expression could make tissue specificity based predictions a thing of the past and revolutionize the field of functional genomics. (sciencedaily.com)
Heat shock proteins (HSPs) and antioxidant enzymes are the two major classes of functional proteins that are induced during HSR. (frontiersin.org)
By CRISPR-Cas9 genome editing, the researchers developed mutant plants that didn't contain functional TPC1, TPC2, or TPC3 genes and double mutant plants that lack functions of both TPC2 and TPC3 genes. (sciencedaily.com)
The mutant that lacked a functional TPC1 gene showed no SV channel activity. (sciencedaily.com)

Whithout mutations we would know very little about inheritance and the existence of genes. (intechopen.com)
Mutations in the GLDC gene account for about 80 percent of all cases of nonketotic hyperglycinemia. (medlineplus.gov)
Some GLDC gene mutations lead to the production of a nonfunctional version of glycine dehydrogenase, while other mutations reduce but do not eliminate the enzyme's activity. (medlineplus.gov)
By the mid 1980s, techniques largely had switched from those of the biochemistry of intermediates and enzymes to the identification of mutations in genes. (medscape.com)
Data from the Human Genome Project surely will be useful in identifying mutations in the thousands of genes that must underlie inherited diseases of the central and peripheral nervous system. (medscape.com)
Until the genes and their mutations that underlie neurological disease are characterized, inherited disorders have to be defined the way clinicians have been classifying disease over the last 2 centuries. (medscape.com)
Sequence analysis detects mutations in 95% of males and 95% of female carriers. (medscape.com)
Several mutations in the OCRL gene have been described, including truncation mutations, missense mutations, and large deletions. (medscape.com)

A positionally conserved ORF has been found on the complementary strand of the cox1 genes of both eukaryotic mitochondria (protist, plant, fungal and animal) and alpha-proteobacteria. (biomedcentral.com)
This search has led to the observation of an unknown, positionally conserved open reading frame (ORF) on the complementary strand of eukaryotic cox1 genes. (biomedcentral.com)

This technology enables the targeted, highly specific and highly sensitive simultaneous quantitative measurement of 92 proteins in 88 samples. (unimedizin-mainz.de)
The Report for "Global Anatomic Pathology Market" by Market Research Future Comprises of Extensive Primary Research along with the Detail Analysis of Qualitative as well as Quantitative Aspects by Various Industry Experts, Key Opinion Leaders to Gain a Deeper Insight of the Market and Industry Performance. (medgadget.com)

Here, using recombinant complex reconstitution and transcriptome-wide analysis, we showed that the EJC peripheral protein complexes ASAP and PSAP form distinct complexes with the EJC core and can confer to EJCs distinct alternative splicing regulatory activities. (nature.com)
To determine how the ASAP complex interacts with the EJC core, we performed in vitro coprecipitation assays 3 with Tandem Affinity Purification (TAP)-tagged recombinant proteins corresponding to full-length human SAP18, RNPS1 and ACINUS. (nature.com)
Individual ASAP proteins were incubated with recombinant EJC core proteins either in the presence or absence of ssRNA and ADPNP. (nature.com)
Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris. (lu.se)

Their results showed that genes from the same Gene Ontology 'Biological Process' (GO-BP) category - which indicates the physiological function of their encoded protein, such as 'response to temperature' or 'amino acid metabolism' - are transcriptionally co-regulated, independent of the tissue in which they are expressed. (sciencedaily.com)
Such studies involve analyzing differences in glycan structure on cell type (tissue), sex, and age, as well as changes associated with perturbations as a result of gene knockout of glycan biosynthesis-related enzyme, disease and drug treatment. (nih.gov)
We demonstrate MetaNetwork's utility by employing it to identify groups of proteins associated with prostate cancer from a proteomic analysis of tumor and adjacent normal tissue samples. (stanford.edu)

pp)pGpp induction resulted in the downregulation of many genes involved in protein and RNA/DNA metabolism. (nih.gov)
Interestingly, most mRNAs involved in lipid metabolism are overexpressed after hatching (FASN, SCD1, ACOX1), whereas genes implicated in carbohydrate metabolism (HK1, GAPDH, GLUT1) and development (HGF, IGF, FGFR2) are predominantly overexpressed from E12 to E20. (biomedcentral.com)

In America, if top-down solutions remain stalled, the needed change can only occur through better health decisions based on more valid measurements and analyses that improve medical decisions and the health of individuals and populations. (jci.org)
The protein measurements are carried out on Dynamic Array chips from Fluidigm (Figure 1) with integrated fluidic circuits (IFC). (unimedizin-mainz.de)
Protein diffusion in living cells is effectively measured by fluorescence correlation spectroscopy (FCS), a fluctuation-based approach that allows to carry out measurements at a physiological protein expression level. (uni-marburg.de)

Then, by patch-clamp electrophysiology analyses, they measured the ionic currents in isolated vacuoles from the living cells of M. polymorpha plants. (sciencedaily.com)

The speciation of M. scandinavica was determined by 16S rRNA gene sequencing. (wikipedia.org)
The complete mt genome is 14,373 bp in size and comprises 36 genes, including 12 protein-coding, two rRNA and 22 tRNA genes. (nature.com)

59/99 for heat), and overall GWAS candidates were more likely to be differentially expressed than other genes. (frontiersin.org)

The number of mitochondrial protein genes is believed to vary from 3 to 67, while the tRNA gene content varies from 0 to 27 [ 2 ]. (biomedcentral.com)

Despite a disparity in gene content, all mtDNAs encode at least two components of the mitochondrial electron transport chain, including cytochrome c oxidase I (Cox1). (biomedcentral.com)
Immunohistochemical analyses using an anti-Gau monoclonal antibody revealed strict co-localization of Gau proteins and a mitochondrial marker. (biomedcentral.com)
Studies of the gau ORF will shed light on the origin of novel genes and their functions in organelles and could also have medical implications for human diseases that are caused by mitochondrial dysfunction. (biomedcentral.com)
The mitochondrial genes are arranged compactly and generally have no introns and few intergenic nucleotides. (biomedcentral.com)
The mitochondrial gene content is highly variable across eukaryotes. (biomedcentral.com)

TCP proteins are plant-specific transcription factors implicated to perform a variety of physiological functions during plant growth and development. (uncg.edu)
PARP-1 and PARP-2 belongs to a large family of enzymes that, using NAD+ as a substrate, synthesizes and transfers ADP-ribose onto aspartic and glutamic acid residues of acceptor proteins, including PARPs, histones, transcription factors and DNA repair proteins. (imim.es)

Cyanobacterial phycobilisome (PBS) pigment-protein complexes harvest light and transfer the energy to reaction centers. (osti.gov)
RNA-seq based identification and mutant validation of gene targets related to ethanol resistance in cyanobacterial Synechocystis sp. (biomedcentral.com)

We further identified changes in module eigenproteins indicative of dysregulation in protein translation and trafficking pathways. (stanford.edu)

A highly conserved yeast homologue of P1W1 protein was shown to act biochemically as would be expected for a protein with a role in RNA processing, as predicted by the algorithm. (sciencedaily.com)
Background: Plant bZIP proteins characteristically harbor a highly conserved bZIP domain with two structural features: a DNA-binding basic region and a leucine (Leu) zipper dimerization region. (datadryad.org)

In yeast and other simple organisms, the expression of genes with similar functions tends to be coordinately regulated. (sciencedaily.com)
To this end, a kinetic study was designed to determine the level of expression of selected genes involved in steatosis-related liver functions throughout embryogenesis. (biomedcentral.com)
The selected candidate will become an integral part of a research project focused on exploring the non-canonical functions of two complement proteins, C3 and CD59, in the pathophysiology of pancreatic β-cells, particularly in their connection to diabetes development. (lu.se)

Thus, PARP-1 might plays a critical role as a signaling molecule, which controls the expression of multiple genes involved in the inmmune response. (imim.es)
MATERIALS AND METHODS: We tested A. montana mother tincture and a range of homeopathic dilutions in different human and murine cell culture models to demonstrate their anti-inflammatory properties by measuring the inflammatory markers: tumor necrosis factor alpha (TNFα), interleukin-6 (IL-6), cyclooxygenase-2 (COX-2), monocyte chemoattractant protein-1 (MCP-1), intercellular adhesion molecule (ICAM-1), reactive oxygen species (ROS) and cell migration. (bvsalud.org)

In humans, the proteome comprises at least 20,000 unmodified ('canonical') proteins. (unimedizin-mainz.de)

Here, we present the primary structures of ~30,000 unique proteoforms, nearly 10 times more than in previous studies, expressed from 1690 human genes across 21 cell types and plasma from human blood and bone marrow. (stanford.edu)

Despite an evolutionary distance of at least 1.5 to 2.0 billion years, the deduced Gau proteins share some conserved amino acid signatures and structure, which suggests a possible conserved function. (biomedcentral.com)
Glycine is an amino acid, which is a building block of proteins. (medlineplus.gov)
A change in structure was in those days thought to be due to a change in an amino acid somewhere in the peptide chain of the protein. (medscape.com)
Phylogenetic tree based on the alignment of the surface layer protein A amino acid sequence of Clostridium difficile 027 (GenBank accession no. (cdc.gov)
Amino acid comparisons were accomplished by using ClustalW ( www.ebi.ac.uk/clustalw ), and the output was used for construction of the phylogenetic tree by TreeView version 1.6.6 ( http://en.bio-soft.net/tree/TreeView.html ). (cdc.gov)

Interestingly, carbohydrate-related genes appear to be highly expressed at the start of kinetics, while most lipid-related genes are overexpress after hatching, revealing greater sensitivity to the food transition that occurs at this stage. (biomedcentral.com)

This hypothesis could be tested by purifying the gau gene product and determining its sequence. (biomedcentral.com)
All PCR-ribotype 027 strains showed the same slp A gene nucleotide sequence. (cdc.gov)

Hence, we could test whether individual ASAP proteins interact with EJC core components individually or with the EJC core complex. (nature.com)

This protein is one of four enzymes that work together in a group called the glycine cleavage system. (medlineplus.gov)
Although direct identification of abnormal protein structure still was not possible, such defects could be inferred, indirectly, by demonstrating alterations in the kinetic properties of patients' enzymes (ie, rates of reaction as concentrations of cofactor or substrate were varied) or in the rate at which heating the enzyme altered its catalytic properties. (medscape.com)

Skruzny M, Pohl E, Gnoth S, Malengo G, Sourjik V (2020) The protein architecture of the endocytic coat analyzed by FRET microscopy. (uni-marburg.de)

Our analysis showed that mRNA abundances, gene organization (operon) and gene essentiality are correlated in Buchnera (i.e., the most expressed genes are essential genes organized in operons) whereas no link between mRNA abundances and gene strand bias was found. (biomedcentral.com)

[ 3 ] Proteomic analysis of urine from patients with Lowe syndrome typically show low levels of megalin and cubilin denoting a decrease in the number of these multiligand receptors in the PTC. (medscape.com)
Purification and Proteomic Analysis of Plant Plasma Membranes. (lu.se)

However, the microarray data provided a wealth of information on which proteins to examine in the proteome gels and has given new insights into the physiological activities of the cells under stressful conditions leading to arrest of fermentation. (avf.org)
To examine the physiological consequences of GRF expression, we have established strains of transgenic mice containing a fusion gene including the promoter/regulatory region of the mouse metal-lothionein-I (MT-I) gene 13 and the coding region of the human GRF gene 12 . (northwestern.edu)

We analyzed the slp A gene of these strains by using the National Center for Biotechnology Information BLAST server ( www.ncbi.nlm.nih.gov/blast )and the European Bioinformatics Institute ClustalW server ( www.ebi.ac.uk/clustalw ). (cdc.gov)
The slp A genes of all strains were amplified by PCR mapping. (cdc.gov)

Tagging these proteins with a fluorescent marker, they studied their localization in M. polymorpha cells. (sciencedaily.com)

The asymmetry of the distribution of the genes between the two strands of DNA is explained as being a means of minimizing interruptions in gene transcription due to collisions between the DNA and RNA polymerases. (biomedcentral.com)
In some mtDNAs (e.g., nematodes and annelids), all genes are transcribed in the same direction, whereas, in others, both strands encode genes. (biomedcentral.com)

Emma Lundberg's group has a wealth of experience in using microscopy to yield biological images that paint a picture of this cell-to-cell heterogeneity of protein expression information, and joining her lab has deepened my expertise in integrating datasets to perform innovative analyses of single-cell protein expression. (stanford.edu)

Understanding the mechanisms involved in guiding at least under homeostatic or physiological conditions. (lu.se)

Survival analysis revealed that a low NRP1 expression in adrenocortical carcinoma (ACC), cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC), low-grade glioma (LGG), and stomach adenocarcinoma (STAD) was associated with poor prognosis. (hindawi.com)
Neurovascular-related NRP family genes are significantly correlated with cancer prognosis, TME, and immune infiltration, particularly in BLCA. (hindawi.com)

Unexpectedly, female transgenic mice carrying the MT-GRF fusion gene are fertile, in contrast to female transgenic mice expressing human or rat growth hormone, which are generally infertile. (northwestern.edu)

Anatomy3

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