The 3-alkyne probe showed the highest photo-affinity labeling efficiency. (elsevierpure.com)
The NMR-derived conformation of SP in GM1 bicelles is docked on homology modeled NK(1)R and resulting interactions satisfy reported mutagenesis, fluorescence, photo-affinity labeling and modeling data. (rcsb.org)
4-Fluoro-3-nitrophenyl azide FNPA is used for photo affinity labeling. (soltecventures.com)
Proteomics1
IsoTag has the potential to enhance any proteomics platform that employs chemical labeling for targeted protein identification, including isotope-coded affinity tagging, isobaric tagging for relative and absolute quantitation, and chemical tagging strategies for post-translational modification. (vectorlabs.com)
Probe10
The first part of the talk focuses on the design, synthesis, and study of a photoaffinity probe for Aβ oligomers - crystal violet diazirine (CrVD). (uci.edu)
To address this, CrVD was designed as an isosteric analogue with a photoaffinity labeling probe, and synthesized from α,α,α-trifluoroacetophenone. (uci.edu)
When it is applied to a ligand-receptor system using a photolabile ligand probe, it provides an opportunity for the identification of the receptor molecule, as well as for the identification of the portion of the receptor that is labeled and even the identification of spatially approximated residues within the docked ligand and receptor. (pancreapedia.org)
In this report, we describe the step-by-step procedures of photoaffinity labeling of the secretin receptor using the [Bpa 16 ,Tyr 10 ]rat secretin-27 probe (1) as an example. (pancreapedia.org)
The steps covered include probe characterization by binding and biological activity assays, probe radioiodination, receptor photoaffinity labeling, peptide mapping of the labeled receptor for identification of candidate fragments and radiochemical sequencing for identification of the specific receptor residue that was covalently labeled. (pancreapedia.org)
The superior NADase inhibitor, 8-azidoadenosyl pseudocarba-NAD, was characterized kinetically and shown to fulfill the criteria required of a specific active site directed photoaffinity probe. (utoledo.edu)
Labeling with a S1' photoactivatable, activity-based probe and recombinant MMP-12 catalytic domain resulted in a 1:1 ratio. (rug.nl)
Furthermore, the labeled protein could be enriched with monomeric avidin beads based on the biotin residue that forms part of the probe. (rug.nl)
i) metabolic labeling with a chemically functionalized glycan, (ii) chemical tagging and enrichment using an isotopic recoding affinity probe, (iii) directed tandem MS, and (iv) targeted glycopeptide assignment (Figure 2). (vectorlabs.com)
Isotopic recoding is accomplished by metabolic labeling of cell or tissue samples with azide- or alkyne-functionalized sugars, followed by chemical conjugation with a biotin probe bearing a unique isotopic signature. (vectorlabs.com)
Peptide3
Direct analysis of mode of peptide docking using intrinsic photoaffinity labeling has provided detailedinsights for the molecular basis of cholecystokinin (CCK) interaction with the type 1 CCK receptor. (monash.edu)
Dong, M & Miller, LJ 2013, ' Direct demonstration of unique mode of natural peptide binding tothe type 2 cholecystokinin receptor using photoaffinity labeling ', Peptides , vol. 46, pp. 143-149. (monash.edu)
Photoaffinity labeling with a fluorescent nonpolar peptide caused the covalent attachment of the peptide to a single site on the Tsp protein. (embl.de)
Receptor7
In this report, we utilize the photoaffinity labeling of the secretin receptor as an example of the application of this technique. (pancreapedia.org)
We have utilized photoaffinity labeling to identify multiple residue-residue approximations between secretin and its receptor, providing extremely useful constraints for building a molecular model of the ligand-bound secretin receptor (1). (pancreapedia.org)
They are often used in photoaffinity labeling studies to observe a variety of interactions, including ligand-receptor, ligand-enzyme, protein-protein, and protein-nucleic acid interactions. (wikipedia.org)
Diazirines are widely used in receptor labeling studies. (wikipedia.org)
Using receptor photoaffinity labeling experiments, we were able to show that ARA9 enhances AHR signal transduction by increasing the available AHR binding sites within the cytosolic compartment of the cell. (nih.gov)
Use of dense amino acid labeling to determine the influence of hormone on the receptor half-life and the rate of appearance of newly synthesized receptor. (nih.gov)
Among these ligands, GAT100 emerged to be the most potent, first-in-class analog which labels the receptor covalently. (northeastern.edu)
Affinity4
The inhibitory effect of P-ATP was specific for ATP, and resulted from affinity labeling in that it was long-lasting, was not reversed by washing, was related in magnitude to exposure period and was attenuated by ATP present during the incubation. (cdc.gov)
In the context of new methods to study biology, chemical methods uniquely offer the ability to develop new reactive warheads, optimize bioorthogonal chemistries, and label a protein-of-interest with a (multi)functional group of choice, including natural or non-natural functionalities harbouring a recognition, reporting or affinity handle. (nature.com)
Two photo-crosslinking biarsenical (CrAsH-EDT 2 )-modified probes were synthesized that are expected to be useful tools for tetracysteine-labeled proteins to facilitate the co-affinity purification of their DNA binding sequences and interacting proteins. (umn.edu)
It is a high affinity binding protein for the antiischemic phenylalkylamine Ca2+ antagonist [3H]emopamil and the photoaffinity label [3H]azidopamil. (nih.gov)
Diazirine3
A common problem with diazirine photo-labeling is formation of diazo species that release carbenes with a time lag allowing diffusion away from the intended binding site and off-target binding. (enamine.net)
Mass spectrometric analysis established that crystal violet diazirine selectively labels a synthetic trimer previously developed in our laboratory. (uci.edu)
This results in proximity dependent labeling of other species with the diazirine containing compound. (wikipedia.org)
Probes5
A series of photolabile CCK analog probes withsites of covalent attachment extending from position 26 through 32 were characterized, with the highestaffinity analogs that possessed full biological activity utilized in photoaffinity labeling. (monash.edu)
Photoaffinity label derivatives of fluorene as probes in in vitro carc" by W E. White, A M. Sarrif et al. (jax.org)
Photoaffinity label derivatives of fluorene as probes in in vitro carcinogenesis. (jax.org)
Belharra says those processes use photoaffinity labeling , optical techniques where chemical components in proteins respond to light-activated probes, in this case the ligands or binding sites that are otherwise missed. (technewslit.com)
The company says since starting up in 2021, it compiled a library of of these photoaffinity probes that explore interactions between proteins and ligands in their native cellular environments. (technewslit.com)
Synthesis1
The synthesis of a fluorescent photoaffinity labelling agent and of two tin-containing thymol analogues is also described. (unipi.it)
Proteins2
These labels are used primarily to identify binding sites on proteins. (nih.gov)
1:1 ratio of labelling ribosomal proteins and RNAs. (medpdfarticles.com)
Protein1
Identification of the bile acid-binding site of the ileal lipid-binding protein by photoaffinity labeling, matrix-assisted laser desorption ionization-mass spectrometry, and NMR structure. (genome.jp)
Receptors1
14. Photoaffinity labeling of thyroid hormone nuclear receptors. (nih.gov)
Diazirines2
Diazirines are the smallest molecules used in photochemical cross-linking and photoaffinity labeling approaches. (enamine.net)
Yet, as this feature minimizes unspecific labeling, it is actually an advantage of using diazirines. (wikipedia.org)
Recombinant1
Photoaffinity labeling demonstrated direct binding of NAADP to recombinant HN1L/JPT2. (bvsalud.org)
Antagonist1
P-ATP) on contractile responses of the guinea pig vas deferens to ATP was characterized and compared to the effects of the specific P2x-purinoceptor photoaffinity label antagonist, arylazido aminopropionyl ATP (ANAPP3). (cdc.gov)
ATPase1
Tightly bound 2-azido-adenine nucleotides at catalytic and noncatalytic sites of the rat liver F1 ATPase label adjacent tryptic peptides of the beta subunit. (nih.gov)
Irradiation1
Benzophenones, which form reactive triplet carbonyl species upon irradiation, often require long periods of irradiation which can result in non-specific labeling, and moreover are often inert to various polar solvents. (wikipedia.org)
Enrichment2
Photoaffinity-labeling, absolute quantification by mass-spectrometry in the Selected Reaction Monitoring (SRM) mode, and activity-based enrichment techniques were investigated. (rug.nl)
IsoTaG is performed by isotopic recoding and enrichment of metabolically labeled glycoproteins followed by directed tandem MS (MS2 or MSn) analysis and intact glycopeptide assignment. (vectorlabs.com)
Reactive1
Photoaffinity labeling compound for labeling antibodies with aryl nitrene as the reactive group. (soltecventures.com)
Experiments1
tetracycline into ribosomal RNAs under conditions optimized to However, experiments performed with chimeric ribo- avoid non-specific binding and labelling due to tetracycline somal subunits demonstrate that peptidyltransferase photoproducts. (medpdfarticles.com)
Analytical1
In turn, this has created demand for new regioselective chemistries and analytical methods to assess biomacromolecule labelling in the complex environment of the cell. (nature.com)
Chemistry1
This Guest Edited Collection aims to bring together research across the broad remit of covalent biomacromolecule labelling chemistry. (nature.com)
Molecules1
Photoaffinity labeling is a powerful technique for the identification of interacting molecules. (pancreapedia.org)
Identification1
Identification of ligand-binding regions of P-glycoprotein by activated-pharmacophore photoaffinity labeling and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry. (boku.ac.at)
Monomeric1
CrVD also labels full-length Aβ and it differentiates between monomeric or oligomeric Aβ and fibrillar Aβ. (uci.edu)
Direct1
Direct photoaffinity labeling of Kir6.2 by [gamma-(32)P]ATP-[gamma]4-azidoanilide. (ox.ac.uk)
Studies1
At least two separate carrier systems for hepatic uptake of organic cations have been identified through kinetic and photoaffinity labeling studies. (nih.gov)
Specifically2
Interestingly, SUR1, which can be strongly and specifically photoaffinity labeled by 8-azido-ATP, was not photoaffinity labeled by ATP-AA. (ox.ac.uk)
Anti-OBP2 is specifically labeled in DWPS-II, whereas the anti-OBP1 shows a broad spectrum of immunoactivity toward four different sensilla (LSB, SP, ST-I and ST-II). (ijbs.com)