• Two arginines in this domain are responsible for hydrogen bonding phosphotyrosine residues on an Ac-LYASSNPApY-NH2 peptide in the juxtamembrane region of the insulin receptor. (wikipedia.org)
  • The selectivity of an individual SH2 domain is not sharply defined, and a range of residues is typically tolerated at each site following the phosphotyrosine. (lu.se)
  • Residues from αB-helix and the EF and BG-loops are involved in binding of side chains C-terminal to phosphotyrosine in the ligand. (lu.se)
  • Insulin receptor substrate-1 proteins contain both a pleckstrin homology domain and a phosphotyrosine binding (PTB) domain. (wikipedia.org)
  • The genes of the human low Mr phosphotyrosine protein phosphatase (PTPase) isoforms 1 (IF1) and 2 (IF2) were isolated by screening a human placenta cDNA library, cloned in pGEX and expressed in E. coli as fusion proteins with glutathione S-transferase. (unifi.it)
  • SH2 domains are phosphotyrosine recognition domains, often mediating transient interactions with target proteins. (eu.org)
  • This PhosphoDetect™ Anti-Phosphotyrosine Rabbit pAb is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of Phosphotyrosine. (emdmillipore.com)
  • Description: A sandwich ELISA kit for detection of Dual Adaptor Of Phosphotyrosine And 3-Phosphoinositides from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (scalegen.com)
  • 4G10™ used Phosphotyramine coupled to KLH and PY20 used phosphotyrosine conjugated to carrier protein. (fishersci.com)
  • In molecular biology, Phosphotyrosine-binding domains are protein domains which bind to phosphotyrosine. (wikipedia.org)
  • The phosphotyrosine-binding domain (PTB, also phosphotyrosine-interaction or PI domain) in the protein tensin tends to be found at the C-terminus. (wikipedia.org)
  • The phosphotyrosine-binding domain of insulin receptor substrate-1 is not related to the phosphotyrosine-binding domain of tensin. (wikipedia.org)
  • The tensin family member cten (C-terminal tensin like) is an Src homology 2 (SH2) and phosphotyrosine binding domain-containing focal adhesion molecule that may function as a tumor suppressor. (rupress.org)
  • Erythropoietin-induced recruitment of Shc via a receptor phosphotyrosine-independent, Jak2-associated pathway. (uchicago.edu)
  • The discovery of phosphotyrosine phosphatases that contain SH2 domains suggests roles for these molecules in growth factor signaling pathways. (drugbank.com)
  • The human phosphotyrosine signaling network: Evolution and hotspots of" by Lei Li, Chabane Tibiche et al. (uwo.ca)
  • We found that Syp, a phosphotyrosine phosphatase, widely expressed in all tissues in mammals became phosphorylated on tyrosine after stimulation with Epo in M07ER cells engineered to express high levels of human EpoR. (drugbank.com)
  • Expression, purification and preliminary crystal analysis of the human low Mr phosphotyrosine protein phosphatase isoform 1. (unifi.it)
  • These results indicate that the phosphotyrosine protein phosphatase activity of CD45 is critical to its biological function and that bivalent (i.e. uncross-linked) anti-CD45 antibodies can give rise to markedly different responses. (mssm.edu)
  • The right hand Figure shows two work flows utilizing anti-phosphotyrosine antibodies to locate sites of tyrosine. (yale.edu)
  • Description Immunohistochemistry analysis using Mouse Anti-Phosphotyrosine Monoclonal Antibody, Clone G104 (56494). (qedbio.com)
  • Primary Antibody: Mouse Anti-Phosphotyrosine Monoclonal Antibody (56494) at 1:100 for 1 hour at RT. (qedbio.com)
  • Description Western Blot analysis of Human A431 cell lysates showing detection of Phosphotyrosine protein using Mouse Anti-Phosphotyrosine Monoclonal Antibody, Clone G104 (56494). (qedbio.com)
  • The dephosphorylation of the purified hER at phosphotyrosine 537 with a tyrosine phosphatase eliminated binding to an ERE in a gel mobility shift assay. (nih.gov)
  • The bead binding assay was also utilised to screen several phosphatase-stable phosphonotyrosine analogues as replacements for phosphotyrosine in high affinity SH2 binding motifs. (soton.ac.uk)
  • We show that binding of phosphotyrosine peptides to PKM2 results in release of the allosteric activator fructose-1,6-bisphosphate, leading to inhibition of PKM2 enzymatic activity. (rcsb.org)
  • The far right hand work flow enriches for phosphotyrosine peptides after digestion and prior to a Zip Tip clean up step, mass spectrometric analysis and database searching. (yale.edu)
  • In addition, peptide combinatorial library techniques were developed with a unique bead binding assay and a sequencing/ligand identification method capable of determining phosphotyrosine-peptidyl-resin sequences incorporated into the formation of a peptide library containing over one million 6-mer phosphotyrosine-urea peptides, and its screening for binding to the p56 lck SH2 domain. (soton.ac.uk)
  • Antibodies to phosphotyrosine are useful in identifying protein substrates of tyrosine kinase. (qedbio.com)
  • The relationship between cell proliferation, protein tyrosine phosphorylation, phosphotyrosine kinase activity and bradykinin receptor activation in rat mesangial cells was investigated. (nih.gov)
  • Susceptibility of RCMs to ROFA-L-induced cytotoxicity was increased following tyrosine phosphorylation inhibition, suggesting that phosphotyrosine signaling pathways play a critical role in regulating ROFA-L-induced cardiotoxicity. (cdc.gov)
  • Treatments with pertussis-toxin, inhibition of phospholipase C and protein kinase C inhibitors and chelation of free cytosolic calcium did not change the bradykinin-induced inhibition of phosphotyrosine kinase. (nih.gov)
  • 10. Inhibition of signaling through the B cell antigen receptor by the protooncogene product, c-Cbl, requires Syk tyrosine 317 and the c-Cbl phosphotyrosine-binding domain. (nih.gov)
  • Immunogen Phosphotyrosine, alanine and glycine in a 1:1:1 ratio polymerized in the presence of KLH with 1-ethyl-3-(3'- dimethylaminopropyl) carbodiimide. (qedbio.com)
  • We also provide evidence that this regulation of PKM2 by phosphotyrosine signalling diverts glucose metabolites from energy production to anabolic processes when cells are stimulated by certain growth factors. (rcsb.org)
  • This regulation depends upon phosphotyrosine-EphrinB2 signalling repressing c-jun N-terminal kinase 3 activity via STAT1. (nih.gov)
  • prepared by modification of clone PY20 by removing the IgG constant regions which do not contribute to interaction with phosphotyrosine). (researchd.com)
  • Does not cross-react with phosphotyrosine. (qedbio.com)
  • These results suggest that cytoskeleton-associated phosphotyrosine kinase(s) and their substrates may play a role in growth and differentiation of adult intestinal epithelial cells. (rupress.org)
  • The phosphotyrosine-binding domain of insulin receptor substrate-1 is not related to the phosphotyrosine-binding domain of tensin. (wikipedia.org)
  • A critical role for Cbl phosphotyrosine-binding domain binding to Syk phosphotyrosine 323. (nih.gov)
  • When active, Eph receptors initiate a phosphotyrosine-dependent signaling cascade that sustains colorectal cancer cell survival. (nih.gov)
  • Structure-based design of a phosphotyrosine-masked, cell-penetrant small molecule covalently targeting Cys111 on the E3 ligase SOCS2. (expasy.org)
  • Collectively, our results indicate that expression of this phosphotyrosine-binding form of pyruvate kinase is critical for rapid growth in cancer cells. (rcsb.org)
  • Crypt stem cells possess two major phosphotyrosine-containing polypeptides of 36 and 17 kD which have greater than 15 times more phosphotyrosine than that present in the polypeptides of differentiated enterocytes. (rupress.org)