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  • phox
  • Based on crystal structures of three functional domains, we generated a computational structural model of the full p47(phox) protein. (surrey.ac.uk)
  • Using a combination of in silico phosphorylation, molecular dynamics simulation and protein/protein docking, we discovered that the C-terminal tail of p47(phox) is critical for stabilizing its autoinhibited structure. (surrey.ac.uk)
  • Ser-379 phosphorylation disrupts H-bonds that link the C-terminal tail to the autoinhibitory region (AIR) and the tandem Src homology 3 (SH3) domains, allowing the AIR to undergo phosphorylation to expose the SH3 pocket for p22(phox) binding. (surrey.ac.uk)
  • These findings were confirmed by site-directed mutagenesis and gene transfection of p47(phox-/-) coronary microvascular cells. (surrey.ac.uk)
  • Ser-379 phosphorylation is a molecular switch which initiates p47(phox) conformational changes and NADPH oxidase-dependent superoxide production by cells. (surrey.ac.uk)
  • The choanoflagellate Monosiga brevicollis , the unicellular organism that is the closest relatives of multicellular animals, encodes early prototypes of p22 phox , p47 phox as well as the earliest known Nox2-like ancestor of the Nox1-3 subfamily. (biomedcentral.com)
  • p67 phox- and p47 phox -like genes are seen in the sea urchin Strongylocentrotus purpuratus and the limpet Lottia gigantea that also possess Nox2-like co-orthologs of vertebrate Nox1-3. (biomedcentral.com)
  • Analysis of characteristic domains of regulatory subunits suggests a novel view of the evolution of Nox: in fish, p40 phox participated in regulating both Nox1 and Nox2, but after the appearance of mammals, Nox1 (but not Nox2) became independent of p40 phox . (biomedcentral.com)
  • Contains 1 PX (phox homology) domain. (merckmillipore.com)
  • motifs
  • Tim-3 contains no known motifs for the recruitment of inhibitory phosphatases, but it may actually increase signaling downstream of TCR/CD3, at least under acute conditions. (jimmunol.org)
  • Many well represented domains recognize and bind to primary sequences less than 10 amino acids in length called Short Linear Motifs (SLiMs). (biomedcentral.com)
  • bind
  • The resulting Tyr701-phosphoryled STAT1 homodimers translocated to the nucleus and bind to GAS (IFNγ-activated sites) elements to induce expression of IFNγ-regulated genes. (thermofisher.com)
  • The PX domain is required for binding phosphoinositides and can also bind to the third SH3 domain. (merckmillipore.com)
  • interacts
  • The deubiquitinase AMSH (associated molecule with the SH3 domain of signal transducing adaptor molecule) specifically interacts with phosphorylated Xp95 in M-phase cell lysates. (biochemj.org)
  • FRS2 interacts with a region of the juxtamembrane domain of FGFR1 that includes the alternatively spliced VT site. (sdbonline.org)
  • Xenopus
  • By MALDI-TOF (matrix-assisted laser-desorption ionization-time-of-flight) MS analyses and gel mobility-shift assays, Xp95 is phosphorylated at multiple sites within the N-terminal half of the PRD during Xenopus oocyte maturation, and a similar region in Alix is phosphorylated in mitotically arrested but not serum-stimulated mammalian cells. (biochemj.org)
  • molecular
  • This study demonstrates a novel function for the CD4 coreceptor in regulating molecular clustering at the immunological synapse site. (jimmunol.org)
  • The structures of the human amphiphysin-1 SH3 domain complexed with 884 peptides were first modeled using virtual mutagenesis and optimized by molecular mechanics (MM) minimization. (genes2cognition.org)
  • complex
  • Although inhibitory postsynaptic sites lack PSDs, they also consist of complex protein matrices. (frontiersin.org)
  • Another important transcriptional complex that is induced by Type-I IFN is the ISGF3 (ISG factor-3) Complex [6, . (thermofisher.com)
  • The proinflammatory cytokine tumor necrosis factor α (TNF-α) is up-regulated during inflammatory diseases of the central nervous system (CNS), including multiple sclerosis ( 1 ), the AIDS-dementia complex ( 2 ), and Alzheimer's disease ( 3 ). (pnas.org)
  • Repositioning of the MTOC is a complex event dependent on TCR signaling that involves the shortening or sliding of MTs and the subsequent anchoring of MTOC at the site of TCR engagement. (jimmunol.org)
  • The Dictyostelium CARMIL protein links capping protein and the Arp2/3 complex to type I myosins through their SH3 domains. (springer.com)
  • Complex formation results in tyrosine phosphorylation of the Caskin1 SH3 domain. (biomedcentral.com)
  • sequence
  • 14 ) involved the sequence analysis of 424 kb of DNA between the TNF gene cluster and a newly identified gene ∼20 kb telomeric of 0tf-3 at the centromeric end of the class I region. (jimmunol.org)
  • Predicted to cross react with rat (96%), horse (88%), canine (87%) and bovine (87%) based on sequence homology. (merckmillipore.com)
  • cytoskeleton
  • Nck family of adaptors comprising of SH2 and SH3 domains are implicated in the organization of actin cytoskeleton, cell movement, and axon guidance. (biomedcentral.com)
  • isoforms
  • However, the short inserts found in neuron-specific isoforms of Src prevented the binding of tau. (edu.au)
  • Alternative splicing generates several integral-membrane LepR isoforms that possess identical extracellular, transmembrane, and membrane-proximal intracellular domains. (diabetesjournals.org)
  • LepR intracellular domains diverge beyond the first 29 intracellular amino acids, however, with the so-called "short" isoforms (e.g. (diabetesjournals.org)
  • LepRa) containing an additional 3-10 amino acids, and the single "long" isoforms (LepRb) containing a 300-amino acid intracellular tail ( 10 ). (diabetesjournals.org)
  • tissues
  • Many of the effects of leptin are attributable to effects in the central nervous system, particularly in the hypothalamus, but leptin also appears to act directly on some other tissues ( 2 , 3 ). (diabetesjournals.org)
  • lipid
  • Specifically, we questioned whether CD4 association with lipid rafts is critical for TCR/PKCθ clustering at the synapse site. (jimmunol.org)
  • We found that CD4 is not only required for TCR association with lipid microdomains, but is also required for TCR/PKCθ clustering at the synapse site. (jimmunol.org)
  • Fibroblast growth factor-2 (FGF2) induces a specific signaling response within a lipid raft membrane microdomain in human neuroblastoma cells characterized by the tyrosine phosphorylation of a p80 phosphoprotein. (sdbonline.org)
  • novel
  • A novel Arg/Abl-binding protein, ArgBP2, was isolated using a segment of the Arg COOH-terminal domain as bait in the yeast two-hybrid system. (semanticscholar.org)
  • Because this topic has been covered extensively in other relatively recent reviews ( 1 , 2 ), we focus mainly on recent studies of Tim-3, which has attracted substantial preclinical attention of late as a novel therapeutic target for reversal of T cell exhaustion. (jimmunol.org)
  • Inhibition
  • Inhibition of calcineurin activity by cyclosporin A resulted in a complete block of Ca 2+ -dependent dephosphorylation of P-sites 4, 5, and 6 and correlated with a prominent increase in ionomycin-evoked glutamate release. (jneurosci.org)
  • Therefore, 4E-BPs inhibit mRNA translation initiation and phosphorylation by mTORC1 relieves this inhibition. (biomedcentral.com)