• The second major area of investigation is to understand the role of cytoskeleton proteins, desmin and vimentin in smooth muscle contraction and mitochondrial respiration. (jefferson.edu)
  • In recent years research has shown that desmin and vimentin are involved in signaling pathways and they are the primary factors in facilitating the mechanotransduction. (jefferson.edu)
  • In addition, desmin and vimentin cytoskeleton interacts with mitochondria and possibly modulates respiration. (jefferson.edu)
  • Mitochondrial desmin and vimentin interacts with the VDAC and this interaction disrupts VDAC/MtCK/ANT complex formation, thereby inhibiting the ATP synthesis and promoting ROS production under pathological conditions in smooth muscle. (jefferson.edu)
  • My long term goal of this project is to elucidate our understanding of the mechanism by which desmin and vimentin induces mitochondrial and muscle dysfunction under pathological conditions in smooth muscle. (jefferson.edu)
  • Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. (thermofisher.com)
  • Additionally, 1700029J07RIK overexpression attenuated AKT phosphorylation and inhibited Gli2 protein levels, along with cytoskeleton proteins desmin, vimentin, and tubulin. (unf.edu)
  • The RP-box motif in GFAP has the consensus sequence, RLSL-RM-PP. Sequences similar to the GFAP RP-box motif are also to be found in vimentin, desmin and peripherin. (ox.ac.uk)
  • The following product was used in this experiment: Vimentin Monoclonal Antibody (V9) from Thermo Fisher Scientific, catalog # MA5-11883, RRID AB_10985392. (thermofisher.com)
  • However, PLM F enhances the phosphorylation of intracellular vimentin. (wikipedia.org)
  • We herein demonstrated that CADM1 interacts with Hippo pathway core kinases and enhances the phosphorylation of YAP1, and also that the membranous co-expression of CADM1 and LATS2 predicts a favorable prognosis in lung adenocarcinoma. (cancerindex.org)
  • Primary ependymal cell cultures prepared from 10-day-old rats were found by immunohistochemistry to express vimentin but not GFAP and by PCR to express mRNA for Dio2, Gpr50, Darpp-32 and Tsh receptors that are characteristic of tanycytes. (nottingham.ac.uk)
  • These results are supported by previous data, which showed that the dramatic reorganization of GFAP within cells was due to phosphorylation-dephosphorylation of a site located in this RP-box. (ox.ac.uk)
  • Like GFAP, these include phosphorylation and proteolysis sites and are adjacent to the start of the central alpha-helical rod domain, suggesting that this motif of general importance to type III intermediate filament protein assembly. (ox.ac.uk)
  • Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. (thermofisher.com)
  • Subcellular localization of IRS-1 and phosphorylation levels of mitogen-activated protein kinase and Akt1 were also analyzed.IGF-IR was differentially expressed across histologic subtypes (P = 0.04), with highest levels observed in squamous cell tumors. (omicsdi.org)
  • We have previously shown that HMGA2-induced transcription requires phosphorylation of H2A.X at S139, which in turn is mediated by the protein kinase ataxia telangiectasia mutated (ATM) 10 . (nature.com)
  • We performed dual immunofluorescence immunohistochemistry (IHC) using IGFBP-1 and vimentin as decidual and mesenchymal markers, respectively. (uwo.ca)
  • Using immunohistochemistry in a well-characterized set of adenocarcinoma tissues, we showed down-regulation of epithelial markers (E-cadherin and cytokeratin 18) and up-regulation of mesenchymal markers (vimentin and α-smooth muscle actin) with concomitant transforming growth factor-β1 (TGF-β1) expression at the invasive margin compared with the central tumor. (aacrjournals.org)
  • Metastasizing tumor cells show increased expression of the intermediate filament (IF) protein vimentin, which has been used to diagnose invasive tumors for decades. (utwente.nl)
  • Here we show that high-mobility group AT-hook 2 protein (HMGA2) induces DNA nicks at the transcription start site, which are required by the histone chaperone FACT complex to incorporate nucleosomes containing the histone variant H2A.X. Further, phosphorylation of H2A.X at S139 (γ-H2AX) is required for repair-mediated DNA demethylation and transcription activation. (nature.com)
  • Biseugenol-exposure tumors showed acquired epithelial features such as phosphorylation of E-cadherin, cytokeratin-18 and loss mesenchymal signature Snail, but not vimentin regulation. (oncotarget.com)
  • Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. (thermofisher.com)
  • Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. (abcam.cn)
  • I found that activation of CysLT1R via LTD4 increased the translocation and accumulation of β-catenin to the nucleus which induce proliferation of HCT116 colon cancer cells through phosphorylation of GSK-3β and activation of Tcf/Lef. (lu.se)
  • It was also found that LTD4 could induce Epithelial-Mesenchymal Transition (EMT) in SW480 colon cancer cells by i) reducing membrane E-cadherin, ii) increase levels of nuclear β-catenin, iii) increase EMT marker such as vimentin and snail which leads to increase migration of SW480 colon cancer cells. (lu.se)
  • Recent observations indicate that vimentin is not only a passive marker for carcinoma, but may also induce tumor cell invasion. (utwente.nl)
  • The researchers found that miR-450a blocked genes associated with vimentin, a protein in the cell invasion pathway. (fapesp.br)
  • To clarify how vimentin IFs control cell adhesions and migration, we analyzed the nanoscale (30-50 nm) spatial organization of vimentin IFs and cell-matrix adhesions in metastatic fibroblast cells, using three-color stimulated emission depletion (STED) microscopy. (utwente.nl)
  • We also studied whether wild-type and phospho-deficient or -mimicking mutants of vimentin changed the size and lifetime of focal adhesions (FAs), cell shape, and cell migration, using live-cell total internal reflection imaging and confocal microscopy. (utwente.nl)
  • Vimentin expression in these cells caused a reduction in FAs size and an elongated cell shape, but did not affect FA lifetime, or the speed or directionality of cell migration. (utwente.nl)
  • Expression of a phospho-mimicking mutant (S71D) of vimentin increased the speed of cell migration. (utwente.nl)
  • Taken together, our results suggest that in highly migratory, transformed mesenchymal cells, vimentin levels control the cell shape and FA size, but not cell migration, which instead is linked to the phosphorylation status of S71 vimentin. (utwente.nl)
  • These observations are consistent with the possibility that not only levels, but also the assembly status of vimentin control cell migration. (utwente.nl)
  • The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. (cancerindex.org)
  • Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. (abcam.cn)
  • The molecule also decreased tumor growth by inhibiting one mitochondrial gene ( MT-ND2 ) and three nuclear genes ( ACO2 , ATP5B and TIMMDC1 ) involved in one of the stages of cell respiration and in energy production (oxidative phosphorylation). (fapesp.br)
  • In other cell types, IFs are modified by phosphorylation when they are required to be disassembled for example during cell division. (daviddarling.info)
  • Increased Insulin-like Growth Factor Binding Protein-1 Phosphorylation in Decidualized Stromal Mesenchymal Cells in Human Intrauterine Growth Restriction Placentas. (uwo.ca)
  • To test the hypothesis that human IUGR is associated with increased decidual IGFBP-1 phosphorylation at discrete sites, we used IUGR and gestational age matched appropriate for gestational age (AGA) placentas ( n=5 each). (uwo.ca)
  • Together, our data links for the first time, increased decidual IGFBP-1 phosphorylation at discrete sites with human IUGR. (uwo.ca)
  • Furthermore latest results out of Santa Ono's laboratory provides indicated a contribution of histamine released by mast cells to late-phase replies and thereby concentrating on phosphorylation-dependent vimentin disassembly of turned on mast cells may possess a healing value. (morainetownshipdems.org)
  • It has some characteristics in common with the vimentin nonapeptide motif, SSYRRIFGG, including its location in the non-alpha-helical N-terminal domain and a concentration of arginine residues. (ox.ac.uk)
  • Similarly, in vitro experiments showed that CA treatment and 5-LOX siRNA interference inhibited inflammatory activation and NET release in neutrophils, suppressed MAPK and NF-κB phosphorylation in LPS-treated neutrophils, and decreased LTB4 and cfDNA levels. (bvsalud.org)
  • Previous studies have identified a nonapeptide motif, SSYRRIFGG, in the non-alpha-helical N-terminal domain of vimentin that is required for assembly. (ox.ac.uk)
  • The model is overlaid on a transmission electron micrograph of a corresponding assembly mixture of recombinant Xenopus vimentin . (daviddarling.info)