• CnB, the regulatory subunit, contains 4 Ca 2+ -binding EF hand motifs that regulate (through a conformational change) the catalytic function of Cn. (pancreapedia.org)
  • Integrated structural and biochemical analyses revealed that (i) three Ub core-interacting residues are essential for DUB function, (ii) 2-residue-elongated blocking loop 2 regulates substrate selectivity, and (iii) a conserved glutamate residue governs the substrate specificity of PEDV PLP2. (bvsalud.org)
  • In addition to the D1 receptor/PKA/DARPP-32 signaling pathway, D1 receptor stimulation is known to activate Rap1/ERK signaling. (bvsalud.org)
  • Dopamine regulates psychomotor function by D1 receptor/PKA-dependent phosphorylation of DARPP-32. (bvsalud.org)
  • DARPP-32, phosphorylated at Thr34 by PKA, inhibits protein phosphatase 1 (PP1), and amplifies the phosphorylation of other PKA/PP1 substrates following D1 receptor activation. (bvsalud.org)
  • Here, we report that the motor depressant effect produced by the cannabinoid receptor agonist (-)-cis-3-[2-hydroxy-4-(1,1-dimethylheptyl)phenyl]trans-4-(3-hydroxypropyl)cyclohexanol (CP55,940) is attenuated by genetic inactivation of the dopamine- and cAMP-regulated phosphoprotein of 32 kDa (DARPP-32), which is abundantly expressed in the medium spiny neurons of the striatum. (jneurosci.org)
  • The endocannabinoid system has been shown to exert both negative and positive control on striatal dopamine D 2 receptor transmission. (jneurosci.org)
  • In vivo manganese exposure modulates Erk, Akt and Darpp-32 in the striatum of developing rats, and impairs their motor function. (nih.gov)
  • In the striatum, the major component of the basal ganglia, CB1 receptors regulate neurotransmission in various manners. (jneurosci.org)
  • Point mutation of Thr34, the protein kinase A (PKA) phosphorylation site of DARPP-32, produces a similar reduction in the effect of the CB1 agonist. (jneurosci.org)
  • Activation of CB1 receptors, either by an agonist or by inhibition of reuptake of endogenous cannabinoids, stimulates phosphorylation at Thr34, thereby converting DARPP-32 into an inhibitor of protein phosphatase-1. (jneurosci.org)
  • Biochemical and physiological methods were used to determine contractility, Ca 2+ cycling parameters, and protein phosphorylation. (frontiersin.org)
  • This, in turn, prevents dephosphorylation of downstream target proteins, thereby amplifying the effects produced by activation of the cAMP/PKA cascade ( Greengard, 2001 ). (jneurosci.org)