• Sperm intracellular cAMP levels depend on the activity of adenylyl cyclases, mostly SACY, though transmembrane-containing adenylyl cyclases are also present, and on the activity of cyclic nucleotide phosphodiesterases (PDE) whose role is to degrade cAMP into 5'-AMP. (plos.org)
  • Considerably higher resistance against cyclic nucleotide phosphodiesterases compared to dibutyryl- or 8-Br-cAMP (Cat. (biolog.de)
  • Protein kinase activity was 2-5 times greater in the 'head' than in the posterior end of the fluke and in both particulate and supernatant fractions half-maximal activation was obtained at 0.1-0.4 mcM cyclic AMP. (erowid.org)
  • Incubation of fluke heads for 5 min with 1 mM 5HT increased protein kinase activity of both fractions in the absence of added cyclic AMP, but with 5 mcM cyclic AMP protein kinase activity was increased inthe particulate and decreased in the super-natant fraction. (erowid.org)
  • The effect of phosphorylation by cyclic GMP-dependent protein kinase (G-kinase) on the activity of the plasmalemmal Ca2+-transport ATPase was studied on isolated plasma membranes and on the ATPase purified from pig erythrocytes and from the smooth muscle of pig stomach and pig aorta. (portlandpress.com)
  • Cyclic AMP-dependent protein kinase (A-kinase) did not exert such an effect. (portlandpress.com)
  • G-kinase, but not A-kinase, stimulated the phosphorylation of PI to phosphatidylinositol phosphate (PIP) in a preparation of (Ca2+ + Mg2+)-ATPase obtained by calmodulin affinity chromatography from smooth muscle, but not in a similar preparation from erythrocytes. (portlandpress.com)
  • Adenosine inhibited both the phosphorylation of PI and the stimulation of the (Ca2+ + Mg2+)-ATPase by G-kinase. (portlandpress.com)
  • Evidence is presented showing that the apparent phosphorylation occurs in a contaminant protein, possibly myosin light-chain kinase. (portlandpress.com)
  • It is proposed that G-kinase stimulates the plasmalemmal Ca2+ pump of smooth-muscle cells indirectly via the phosphorylation of an associated PI kinase. (portlandpress.com)
  • These results suggest that NO contributes to L-LTP by stimulating guanylyl cyclase and cGMP-dependent protein kinase, which acts in parallel with PKA to increase phosphorylation of the transcription factor CREB. (jneurosci.org)
  • We recently showed phosphorylation of Drosophila Plexin A by cyclic adenosine monophosphate (cAMP)-dependent protein kinase (PKA) and subsequent inhibition of plexin-mediated repulsive guidance. (stanford.edu)
  • Here we describe how phosphorylation of Plexin A can be detected and quantitated using an in vitro kinase assay and radioactive [γ-P32] adenosine 5'-triphosphate (ATP). (stanford.edu)
  • Activator of protein kinase A (cyclic AMP agonist). (biolog.de)
  • This increase of cyclic AMP leads to the activation of protein kinase A, which inhibits the phosphorylation of myosin and lowers intracellular ionic calcium concentrations, resulting in relaxation. (guidelinecentral.com)
  • Moreover, CG stimulated the phosphorylation of AMP-activated protein kinase (AMPK), and the protective effect of CG on hepatocytes was partially reversed both by the inhibitor of AMPK signaling pathway and overexpression of AMPK-DN. (bvsalud.org)
  • The phosphorylation of synapsin I depends on cyclic adenosine monophosphate and type II calcium/calmodulin protein kinase. (diva-portal.org)
  • The reaction continues when cAMP activates protein kinase which catalyzes phosphorylation, activating a ribonucleic acid (RNA) polymerase. (nfpt.com)
  • Exposure of the amnion fibroblasts to cortisol led to the activation of the cyclic adenosine monophosphate (cAMP)-protein kinase A (PKA) pathway, which induced the phosphorylation of the kinase SRC and STAT3 (signal transducer and activator of transcription 3). (elsevierpure.com)
  • Stimulation of the CaSR by agonists activated several pathways, which were detected by measuring accumulation of the second messengers D-myo-inositol 1-phosphate (IP 1 ) and cyclic adenosine 3',5'-monophosphate (cAMP), and by measuring the phosphorylation of extracellular signal-regulated kinase 1 and 2 (ERK1/2). (nyu.edu)
  • We furthermore demonstrate that p110δ is phosphorylated at residues Y524 and S1039 and that phosphorylation requires an intact binding site for PI3-kinase in c-Kit/D816V. (ku.dk)
  • TGFα, T 4, and 8-Br-cAMP each caused protein kinase A (PKA) II serine phosphorylation, whereas phosphorylation of PKA-II was not seen in cells treated with EGF or 8-chloro-cyclic adenosine monophosphate. (tmu.edu.tw)
  • When D1 receptors are activated, it causes a release of the secondary messenger cAMP (cyclic adenosine monophosphate) which activates the PKA protein (protein kinase A) and leads to the phosphorylation of target proteins (4). (celtarys.com)
  • As in somatic cells, sperm intracellular cAMP concentration is regulated by the opposite action of two enzymes: the adenylyl cyclases (AC), which synthesize cAMP from ATP, and the phosphodiesterases (PDE), which break the phosphodiester bond of cAMP to form 5'-adenosine monophosphate (AMP). (plos.org)
  • Anticholinergics prevent the increases in intracellular concentration of cyclic guanosine monophosphate (cyclic GMP) which are caused by interaction of acetylcholine with the muscarinic receptor on bronchial smooth muscle. (theodora.com)
  • Activation of beta 2 -adrenergic receptors on airway smooth muscle leads to the activation of adenylyl cyclase and to an increase in the intracellular concentration of cyclic-3',5'-adenosine monophosphate (cyclic AMP). (theodora.com)
  • Calcium is also involved in the action of other intracellular messengers, such as cAMP (cyclic adenosine monophosphate) and inositol 1,4,5-triphosphate, and thus mediates the cellular response to numerous hormones, including epinephrine , glucagon , vasopressin (antidiuretic hormone), secretin , and cholecystokinin. (msdmanuals.com)
  • 4-PPBP protects neurons using a mechanism that activates the transcription factor cyclic adenosine monophosphate response element-binding protein (CREB). (wikipedia.org)
  • Overexpression of the complementary DNA for human glutamine:fructose-6-phosphate amidotransferase in mesangial cells enhances glucose-induced fibronectin synthesis and transcription factor cyclic adenosine monophosphate-responsive element binding phosphorylation. (cdna-clone.com)
  • Pyrroloquinoline quinone stimulates mitochondrial biogenesis through cAMP response element-binding protein phosphorylation and increased PGC-1alpha expression. (symptoma.com)
  • Fasciola hepatica were collected from fresh beef livers and particulate fractions of whole flukes, 'tails' or 'heads' (anterior ends) assayed for adenylate cyclase (AC) activity and cyclic AMP concentration using a protein binding assay. (erowid.org)
  • 5HT (Calbiochem) caused a marked concentration-dependent accumulation of cyclic AMP especially in 'heads', maximal activation of AC occurring at i mM, and an accompanying increase in motility of the isolated fluke head. (erowid.org)
  • The degree of activation by cyclic AMP was inversely correlated with endogenous cyclic AMP and 5HT concentration. (erowid.org)
  • Goupil S, Maréchal L, El Hajj H, Tremblay M-È, Richard FJ, Leclerc P (2016) Identification and Localization of the Cyclic Nucleotide Phosphodiesterase 10A in Bovine Testis and Mature Spermatozoa. (plos.org)
  • Adenosine A2A receptor agonist polydeoxyribonucleotide ameliorates short-term memory impairment by suppressing cerebral ischemia-induced inflammation via MAPK pathway. (iasp-pain.org)
  • In the current study, induction of ischemia enhanced the levels of pro-inflammatory cytokines and increased phosphorylation of MAPK signaling factors in the hippocampus and basolateral amygdala. (iasp-pain.org)
  • TGFα-induced MAPK activation was potentiated by 8-bromo-cyclic adenosine monophosphate (8-Br-cAMP) but not 8-chloro-cyclic adenosine monophosphate. (tmu.edu.tw)
  • It is known to act through protein phosphorylation via PRKA and through the activation of guanine nucleotide exchange factors like EPAC. (plos.org)
  • Repairs at the cellular level are regulated by cyclic guanine monophosphate (cGMP). (nfpt.com)
  • Predator odor evokes sex-independent stress responses in male and female Wistar rats and reduces phosphorylation of cyclic-adenosine monophosphate response element binding protein in the male, but not the female hippocampus. (broadinstitute.org)
  • Increased cyclic AMP concentrations are also associated with the inhibition of release of mediators from mast cells in the airway. (guidelinecentral.com)
  • Adenosine A2A receptor agonist, polydeoxyribonucleotide (PDRN), suppresses the secretion of pro-inflammatory cytokines and exhibits anti-inflammatory effect. (iasp-pain.org)
  • Genestein blocked CCK-induced phosphorylation events and amylase secretion while vanadate increased amylase secretion. (johnshopkins.edu)
  • 4-PPBP is a neuroprotective cyclic amine which binds to sigma receptors. (wikipedia.org)
  • These results provide evidence that tyrosine phosphorylation is necessary for amylase release and that signaling through GLP-1 receptors does not mediate amylase release in AR42J cells. (johnshopkins.edu)
  • The disparate effects of T 4 on actions of EGF and TGFα, which share the same cell surface receptor, are mediated by hormone phosphorylation and activation of PKA-II. (tmu.edu.tw)
  • In chronic heart failure following myocardial infarction, dramatic reduction of the microdomain-specific β 2 -AR/cAMP signals and β 2 -AR dependent PLM phosphorylation was accompanied by a pronounced loss of local PDE3 and an increase in PDE2 effects. (dundee.ac.uk)
  • 5-Methoxytryptamine (1 mM, Calbiochem) caused a 4-fold increase in motility similar to 5HT but had a much diminished effect on cyclic AMP and AC acitivity, while tryptamine (Calbiochem), 5 - hydroxydimethyltryptamine (Sigma-Chem. (erowid.org)
  • In the presence of O.i mM 5HT, AC activity in the fluke particles was inhibited by 0.5 mcM LSD, and LSD also antagonized the 5HT stimulated increase in cyclic AMP levels, Adenine, adenosine, histamine (Calbiochem), adrenaline (Mann), tryptophan, dopamine, GTP (Sigma - Chem. (erowid.org)
  • The enhanced levels of cyclic AMP were preserved by both caffeine and papaverine, and NaF up to 5 mM caused a specific stimulation of AC. (erowid.org)
  • This protein is responsible, via phosphorylation, for regulating the amount of transmitter vesicles available for release. (diva-portal.org)
  • This protein has been shown to inhibit the amount of vesicles ready for release in its dephosphorylated state, and releases its inhibitory binding due to phosphorylation. (diva-portal.org)
  • In contrast with previous results of Furukawa & Nakamura [(1987) J. Biochem (Tokyo) 101, 287-290], no convincing evidence for a phosphorylation of the (Ca2+ + Mg2+)-ATPase was found. (portlandpress.com)
  • Cyclic AMP (adenosine 3', 5'-cyclic monophosphate) is an important player in sperm function. (plos.org)