Mutant Chimeric ProteinsPhosphorylationSerineMutationRecombinant Fusion ProteinsMolecular Sequence DataAmino Acid SequenceCell LineTyrosineProtein BindingProtein-Serine-Threonine KinasesPhosphoproteinsProtein KinasesTransfectionProtein Structure, TertiaryMutagenesis, Site-DirectedBase SequenceBinding SitesEnzyme ActivationProtein-Tyrosine KinasesCells, CulturedOxidative PhosphorylationKineticsThreoninePhosphoserineDNA-Binding ProteinsMutant ProteinsEscherichia coliBlotting, WesternSignal TransductionTranscription FactorsMembrane ProteinsSequence Homology, Amino AcidCOS CellsProtein Kinase CPlasmidsProtein Processing, Post-TranslationalRecombinant ProteinsHeLa CellsEnzyme InhibitorsCloning, MolecularCyclic AMP-Dependent Protein KinasesCarrier ProteinsBacterial ProteinsProto-Oncogene ProteinsDNA PrimersCell MembraneProtein TransportAmino Acid SubstitutionPhosphotyrosinePhosphopeptidesChimeraImmunoblottingCricetinaeProto-Oncogene Proteins c-aktPrecipitin TestsTranscription, GeneticSubstrate SpecificityNuclear ProteinsPeptide MappingPhenotypeElectrophoresis, Polyacrylamide GelMitogen-Activated Protein KinasesStructure-Activity RelationshipCell NucleusProteinsCell Line, TumorCytoplasmPhosphothreonineCercopithecus aethiops3T3 CellsSequence DeletionModels, BiologicalTumor Cells, CulturedAmino Acid MotifsModels, MolecularGenetic Complementation TestSequence AlignmentCHO CellsTrans-ActivatorsCasein Kinase IIsrc-Family KinasesIntracellular Signaling Peptides and ProteinsImmunoprecipitationSaccharomyces cerevisiae ProteinsCell Cycle ProteinsCalciumApoptosisGreen Fluorescent ProteinsPhosphoprotein PhosphatasesTime FactorsAdaptor Proteins, Signal TransducingMice, Mutant StrainsBiological TransportPoint MutationProtein ConformationPeptide FragmentsPhosphatidylinositol 3-KinasesMicroscopy, FluorescenceTranscriptional Activation