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Protein KinasesProtein-Serine-Threonine KinasesPhosphatidylinositol 3-KinasesPhosphorylationMAP Kinase Signaling SystemSerineProtein Kinase CCalcium-Calmodulin-Dependent Protein Kinasessrc-Family KinasesTyrosineCyclic AMP-Dependent Protein KinasesProtein-Tyrosine KinasesProtein Kinase InhibitorsPhosphoproteinsEnzyme ActivationMitogen-Activated Protein Kinase 1p38 Mitogen-Activated Protein KinasesMolecular Sequence DataCell LineAmino Acid SequencePhosphoserineMitogen-Activated Protein Kinase 3ThreonineCasein Kinase IIMitogen-Activated Protein KinasesProtein BindingProto-Oncogene Proteins c-aktOxidative PhosphorylationMitogen-Activated Protein Kinase KinasesCDC2 Protein KinaseJNK Mitogen-Activated Protein KinasesCells, CulturedKineticsSignal TransductionExtracellular Signal-Regulated MAP KinasesMutationGlycogen Synthase Kinase 3Blotting, WesternTransfectionPhosphotyrosineCyclin-Dependent KinasesProto-Oncogene ProteinsEnzyme Inhibitorsp21-Activated KinasesCasein KinasesRibosomal Protein S6 KinasesIntracellular Signaling Peptides and ProteinsMAP Kinase Kinase KinasesBinding SitesIsoenzymesRecombinant ProteinsPhosphothreonineProtein Structure, TertiaryeIF-2 KinaseCell Line, TumorMAP Kinase Kinase 1Peptide MappingPhosphoprotein PhosphatasesTetradecanoylphorbol AcetateCalciumProtein Processing, Post-TranslationalSubstrate SpecificityPhosphotransferases (Alcohol Group Acceptor)PhosphopeptidesFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesImmunoblottingPrecipitin TestsRibosomal Protein S6 Kinases, 90-kDaCell Cycle ProteinsRecombinant Fusion ProteinsReceptor Protein-Tyrosine KinasesProtein Kinase C-deltaMAP Kinase Kinase 4rho-Associated KinasesDNA-Binding ProteinsApoptosisI-kappa B KinaseProtein Kinase C-alphaHeLa CellsCreatine KinaseAMP-Activated Protein KinasesCyclic AMPAdaptor Proteins, Signal TransducingMutagenesis, Site-DirectedModels, BiologicalBase SequenceAdenosine TriphosphatePyruvate KinaseProteinsTumor Cells, CulturedMyosin-Light-Chain Kinase3T3 CellsTOR Serine-Threonine KinasesCDC2-CDC28 KinasesAurora KinasesCarrier ProteinsTime FactorsElectrophoresis, Polyacrylamide GelCell Cycle
SubstratesForm a unique fingerprintProteinsMitogen-activaValidated phosphorylation sitesRegulatesPhosphatidylinositolInhibitorsActivationInhibitionResiduesSubstrateSignal TransductionAssaysCreatine kinaseOxidative phosphorylationProtein tyrosine kinaseCAMP-dependSubunitAssaySpecificityPathwayResiduePeptidesPathwaysPhosphorylateCyclin-dependeEnzymesEfficient phosphorylationVitroFamily kinaseVivo phosphorylationActivityRegulateCdk1MyosinGlycolysisIntracellularBiochemicalExtracellularMetabolismActinStimulationEnzymeGlycogenFacilitatesMass spectrometryCellularCellsSignalingSequence
Substrates19
  • Here we compare the specificity of two budding yeast cyclins, the S-phase cyclin Clb5 and the M-phase cyclin Clb2, in the phosphorylation of 150 Cdk1 (Cdc28) substrates. (nature.com)
  • Although we did not identify any highly Clb2-specific substrates, we found that Clb2-Cdk1 possessed higher intrinsic kinase activity than Clb5-Cdk1, enabling efficient phosphorylation of a broad range of mitotic Cdk1 targets. (nature.com)
  • Thus, Clb5 and Clb2 use distinct mechanisms to enhance the phosphorylation of S-phase and M-phase substrates. (nature.com)
  • Identification of a cyclin-cdk2 recognition motif present in substrates and p21-like cyclin-dependent kinase inhibitors. (nature.com)
  • With an attempt to help users efficiently identify the candidate biomarkers in cancers, 30 microarray experiments, including 39 cancerous versus normal cells, were analyzed for detecting cancer-specific expressed genes coding for kinases and their substrates. (nih.gov)
  • In summary, our work provides important insight into the substrates-TTBK2 kinase relationship and suggests that phosphorylation of substrates on multiple sites by TTBK2 is probably involved in the control of ciliogenesis in human cells. (muni.cz)
  • Predikin now consists of two components: (i) PredikinDB, a database of phosphorylation sites that links substrates to kinase sequences and (ii) a Perl module, which provides methods to classify protein kinases, reliably identify substrate-determining residues, generate scoring matrices and score putative phosphorylation sites in query sequences. (biomedcentral.com)
  • The new features significantly enhance the ability of Predikin to analyse protein kinases and their substrates. (biomedcentral.com)
  • Protein kinases and their substrates regulate essentially all cellular processes through complex regulatory networks, in which phosphorylated proteins act as switches that tune the response of the cell to environmental stimuli. (biomedcentral.com)
  • In general, a protein kinase acts on a discrete set of substrates to ensure that signalling fidelity is maintained. (biomedcentral.com)
  • The prediction of peptide specificity is therefore the basis for most of the available computational methods aimed at predicting substrates of protein kinases. (biomedcentral.com)
  • Eukaryotic protein kinases phosphorylate substrates at serine, threonine and tyrosine residues that fall within the context of short sequence motifs. (networkin.info)
  • Knowing the phosphorylation site motif for a protein kinase facilitates designing substrates for kinase assays and mapping phosphorylation sites in protein substrates. (networkin.info)
  • Consensus peptides based on these sequences typically serve as efficient and specific kinase substrates for high throughput screening or incorporation into biosensors. (networkin.info)
  • Protein kinases are critical to cellular signalling and post-translational gene regulation, but their biological substrates are difficult to identify. (biomedcentral.com)
  • Peptide substrates that can enhance lanthanide ion luminescence upon tyrosine phosphorylation enable rapid, sensitive screening of kinase activity, however design of suitable substrates that can distinguish between tyrosine kinase families is a huge challenge. (umn.edu)
  • We used curated proteomic data from endogenous kinase substrates and known Tb 3+ -binding sequences to build a generalizable in silico pipeline with tools to generate, screen, align, and select potential phosphorylation-dependent Tb 3+ -sensitizing substrates that are most likely to be kinase specific. (umn.edu)
  • We demonstrated the approach by developing several substrates that are selective within kinase families and amenable to high-throughput screening (HTS) applications. (umn.edu)
  • Purified bovine DARPP-32 was phosphorylated in vitro by casein kinase II to a stoichiometry greater than 2 mol of phosphate/mol of protein whereas two structurally and functionally related proteins, protein phosphatase inhibitor-1 and G-substrate, were poor substrates for this enzyme. (cornell.edu)
Form a unique fingerprint1
  • Dive into the research topics of 'CD28-mediated cytotoxicity by the human leukemic NK cell line YT involves tyrosine phosphorylation, activation of phosphatidylinositol 3-kinase, and protein kinase C'. Together they form a unique fingerprint. (elsevierpure.com)
Proteins19
Mitogen-activa2
Validated phosphorylation sites1
  • The experimentally validated phosphorylation sites as well as their catalytic kinases were extracted from public resources, and MS/MS phosphopeptides were manually curated from research articles. (nih.gov)
Regulates5
  • Rho-kinase regulates a second pathway that involves activation of LIM-kinases (LIMKs) and subsequent phosphorylation and inactivation of cofilin, an actin dynamizing protein. (uni-muenchen.de)
  • Together, these results show that Rho-kinase regulates the F-actin increase underlying shape change and secretion, but it is not directly involved in aggregation. (uni-muenchen.de)
  • Protein kinase C-dependent phosphorylation regulates the cel. (pubpeer.com)
  • The PI 3-kinase PI3KC2α regulates mouse platelet membrane structure and function independently of membrane lipid composition. (nih.gov)
  • Protein phosphorylation is a biological process that regulates most aspects of cellular life, and the enzymes that catalyze this reaction - protein kinases - constitute the largest family of enzymes encoded by the human genome. (lu.se)
Phosphatidylinositol8
  • Phosphatidylinositol-3 kinase (PI-3K) is an intracellular signal mediator and plays a key role in many cellular functions. (nih.gov)
  • In this study we have examined the changes in lens protein tyrosine phosphorylation and its impact on phosphatidylinositol 3-kinase (PI-3K) signaling during selenite cataract development. (nih.gov)
  • The stimulation of the (Ca2+ + Mg2+)-ATPase activity by G-kinase was only observed in the presence of phosphatidylinositol (PI). (portlandpress.com)
  • G-kinase, but not A-kinase, stimulated the phosphorylation of PI to phosphatidylinositol phosphate (PIP) in a preparation of (Ca2+ + Mg2+)-ATPase obtained by calmodulin affinity chromatography from smooth muscle, but not in a similar preparation from erythrocytes. (portlandpress.com)
  • Enables 1-phosphatidylinositol-3-kinase activity. (nih.gov)
  • Predicted to be part of phosphatidylinositol 3-kinase complex. (nih.gov)
  • Orthologous to human PIK3C2A (phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha). (nih.gov)
  • In this work, we report that CD28-mediated cytotoxicity of YT involves tyrosine phosphorylation and activation of phosphatidylinositol (PI) 3-kinase, the Tec kinase Itk/Emt, and protein kinase C (PKC). (elsevierpure.com)
Inhibitors6
  • We describe a label-free method for the kinase inhibition assay toward discovery of kinase inhibitors. (elsevierpure.com)
  • In conclusion, validation of the on-chip phosphorylation analysis for kinase inhibitors was achieved. (elsevierpure.com)
  • This label-free method might be applied for discovery of kinase inhibitors. (elsevierpure.com)
  • The lysis was inhibited by two different PI 3-kinase inhibitors, wortmannin and LY294002. (elsevierpure.com)
  • Furthermore, exocytosis of cytolytic effector molecules was also inhibited by PI 3-kinase inhibitors and PKC inhibitors. (elsevierpure.com)
  • This cytotoxicity displayed by PMA-treated YT cells, however, could still be inhibited by PI 3-kinase inhibitors and PKC inhibitors. (elsevierpure.com)
Activation25
  • Cell-cycle events are controlled by cyclin-dependent kinases (CDKs), whose periodic activation is driven by cyclins. (nature.com)
  • These studies show that signaling events involving the protein tyrosine phosphorylation process and activation of PI-3K are altered during selenite cataract formation and implicate defects in signal transduction mechanisms as contributing factors in the development of cataract. (nih.gov)
  • In P/Q-type voltage-gated-Ca 2+ channel knock-out mice, in which CaMKII activation was reduced, the levels of Homer3 phosphorylation and the soluble form of Homer 3 were markedly lower. (jneurosci.org)
  • In a heterologous system, the Ca 2+ signaling pattern induced by mGluR1α activation was modulated by the Homer3 phosphorylation state. (jneurosci.org)
  • The signaling pathway Rho-kinase/LIMKs/cofilin phosphorylation during platelet activation and the question, how the phosphorylation of cofilin affects the actin dynamics underlying platelet activation, has not previously been studied. (uni-muenchen.de)
  • It was found that the activation of Rho-kinase is important for an increase in F-actin content underlying Ca2+-independent platelet shape change. (uni-muenchen.de)
  • The activation of Rho-kinase was found to be upstream to secretion and integrin IIbβ3 activation. (uni-muenchen.de)
  • The rapid activation of Rho-kinase during secretion leads to a further increase in F-actin content as compared to shape change. (uni-muenchen.de)
  • Our study shows that LIMK-1 activation was mainly Rho-kinase dependent in LPA- and thrombin-stimulated platelets. (uni-muenchen.de)
  • Like Rho-kinase activation, it was also found that LIMK-1 activation was independent and upstream of integrin IIbβ3 activation. (uni-muenchen.de)
  • Surprisingly, the activation of LIMK-1 failed to increase cofilin phosphorylation during shape change induced by LPA as well as by thrombin. (uni-muenchen.de)
  • Inhibition of the Rho-kinase/LIMK-1 pathway unmasked cofilin dephosphorylation suggesting that during shape change the simultaneous activation of a cofilin phosphatase counteracts the effect of LIMK-1 for phosphorylating cofilin. (uni-muenchen.de)
  • the latter phase was due to Rho-kinase/LIMK-1 activation. (uni-muenchen.de)
  • Moreover, with the premise of Fyn kinase as a paradigm-shifting target in Alzheimer's drug discovery, we explore glucosylpolyphenols as blockers of Aβ-induced Fyn kinase activation, while looking into downstream effects leading to Tau hyperphosphorylation. (whiterose.ac.uk)
  • Several compounds inhibit Aβ-induced Fyn kinase activation and decrease pTau levels at 10 μM concentration, particularly the per-O-methylated glucosylacetophloroglucinol and the 4-glucosylcatechol dibenzoate, the latter inhibiting also butyrylcholinesterase and β-glucosidase. (whiterose.ac.uk)
  • Next-generation sequencing identified two de novo truncating variants in the Src-family tyrosine kinase, LYN , p.Y508*, p.Q507* and a de novo missense variant, p.Y508F, that result in constitutive activation of Lyn kinase. (nature.com)
  • activation of Ras phosphorylation and activation of MAP kinases transmission of signal into genome. (wikipedia.org)
  • PDGF-induced activation of phospholipase C activity is abrogated in cells expressing PLCγ mutated in the tyrosine phosphorylation sites. (wikipedia.org)
  • Phosphorylation of Syk Activation Loop Tyrosines Is Essential for Syk Function. (crossref.org)
  • Pharmacological activation of AMPK by metformin significantly abrogated the loss of RUNX2-S118 phosphorylation and protected from tunicamycin-induced endoplasmic reticulum stress, high glucose-induced in vitro adipogenesis and streptozotocin-induced in vivo bone adiposity and bone phenotype. (uohyd.ac.in)
  • Activation of protein kinase C (PKC) enhances the angiogenic process and is involved in the signaling of vascular endothelial growth factor ( VEGF ). (bvsalud.org)
  • Activation of ROCK by expressing V14RhoA selectively induced CPI-17 phosphorylation without affecting PHI-1 phosphorylation. (uky.edu)
  • Taken together, these results are consistent with a model in which activation of CD28 and LFA-1 induces tyrosine phosphorylation of the CD28 cytoplasmic domain, recruitment and activation of PI 3-kinase, as well as the Tec kinase Itk/Emt, and the activation of PMA-nonresponsive PKC isoenzymes. (elsevierpure.com)
  • Activation of PI 3-kinase and PMA-nonresponsive PKC isoenzymes is shown to be involved directly in cytolytic granule release by YT cells. (elsevierpure.com)
  • CXCL12 induces tyrosine phosphorylation of cortactin, which plays a role in CXC chemokine receptor 4-mediated extracellular signal-regulated kinase activation and chemotaxis. (meharryresearch.org)
Inhibition7
Residues14
  • Phosphorylation of receptor serine/threonine residues has been proposed to exert an inhibitory influence on receptor tyrosine kinase activity and Ser 1327 and Thr 1348 have been identified as specific sites of phosphorylation in the insulin receptor COOH terminal domain. (jci.org)
  • To address the potential negative regulatory role of phosphorylation of these residues in vivo, we assessed the extent of phosphorylation of each site in insulin receptor isolated from the skeletal muscle of 12 NIDDM patients and 13 nondiabetic, control subjects. (jci.org)
  • β-PMA-induced phosphorylation of SERT occurs initially on serine residues(s) and then on threonine residue(s). (aspetjournals.org)
  • The method, named Predikin, identifies key conserved substrate-determining residues in the kinase catalytic domain that contact the substrate in the region of the phosphorylation site and so determine the sequence surrounding the phosphorylation site. (biomedcentral.com)
  • New features in Predikin include the use of SQL queries to PredikinDB to generate predictions, scoring of predictions, more reliable identification of substrate-determining residues and putative phosphorylation sites, extended options to handle protein kinase and substrate data and an improved web interface. (biomedcentral.com)
  • The second factor, termed peptide specificity, describes the interaction between amino acid residues in the catalytic domain of the protein kinase and the substrate residues that surround the phosphorylated residue. (biomedcentral.com)
  • Crystal structures of protein kinases with bound substrate peptides show that substrate residues at positions -3 to +3 relative to the phosphorylated serine, threonine or tyrosine residue adopt an extended conformation and bind to a pocket in the catalytic domain of the protein kinase [ 8 ]. (biomedcentral.com)
  • However, it is recognised that for many protein kinase families, particularly those that phosphorylate Ser/Thr residues, peptide specificity is the major factor that determines substrate specificity. (biomedcentral.com)
  • This method uses a set of peptide mixtures in which each of the 20 amino acid residues is systematically substituted at nine positions surrounding a central site of phosphorylation. (networkin.info)
  • Protein tyrosine kinases (PTKs) catalyze the transfer of the γ-phosphate group from ATP to the hydroxyl group of tyrosine residues, whereas protein tyrosine phosphatases (PTPs) remove the phosphate group from phosphotyrosine. (wikipedia.org)
  • Putative target residues can be predicted by searching for matches to the consensus for a particular kinase. (biomedcentral.com)
  • Sequencing of chymotryptic and thermolytic phosphopeptides from bovine DARPP-32 phosphorylated by casein kinase II suggested that the main phosphorylated residues were Ser45 and Ser102. (cornell.edu)
  • The phosphorylated residues are located NH2-terminal to acidic amino acid residues, a characteristic of casein kinase II phosphorylation sites. (cornell.edu)
  • The glycogen‐associated form of protein phosphatase‐1 (PP‐1 G ) is a heterodimer comprising a 37‐kDa catalytic (C) subunit and a 161‐kDa glycogen‐binding (G) subunit, the latter being phosphorylated by cAMP‐dependent protein kinase at two serine residues (site 1 and site 2). (dundee.ac.uk)
Substrate15
  • Phosphorylation of Clb5-specific targets during S phase was reduced by replacing Clb5 with Clb2 or by mutating the substrate RXL motif, confirming the importance of Clb5 specificity in vivo . (nature.com)
  • Figure 3: Clb5-specific substrate phosphorylation occurs in vivo . (nature.com)
  • Brown, N. R., Noble, M. E., Endicott, J. A. & Johnson, L. N. The structural basis for specificity of substrate and recruitment peptides for cyclin-dependent kinases. (nature.com)
  • Schulman, B. A., Lindstrom, D. L. & Harlow, E. Substrate recruitment to cyclin-dependent kinase 2 by a multipurpose docking site on cyclin A. (nature.com)
  • In this update, we not only enhance the data content in human but also investigate kinase-substrate phosphorylation networks in mouse and rat. (nih.gov)
  • Tyrosine kinase activity in lens membrane preparations was assayed in the presence of a synthetic substrate peptide and [32P]ATP. (nih.gov)
  • Glucose is the major substrate for ATP synthesis through glycolysis and oxidative phosphorylation (OXPHOS), whereas intermediary metabolism through the tricarboxylic acid (TCA) cycle utilizes non-glucose-derived monocarboxylates, amino acids, and alpha ketoacids to support mitochondrial ATP and GTP synthesis. (molvis.org)
  • We have previously described an approach to predicting the substrate specificity of serine-threonine protein kinases. (biomedcentral.com)
  • How a particular protein kinase recognises its substrate protein(s) is therefore a key question. (biomedcentral.com)
  • Two major factors determine the formation of a protein kinase-substrate complex [ 4 ]. (biomedcentral.com)
  • The first, termed substrate recruitment, encompasses any process that increases the effective concentration of the protein kinase substrate. (biomedcentral.com)
  • The relative contribution of substrate recruitment and peptide specificity to protein kinase substrate specificity varies between protein kinases. (biomedcentral.com)
  • Furthermore, the development of lanthanide-based kinase assays is hampered by incomplete understanding of how to integrate sequence selectivity with metal ion binding, necessitating laborious iterative substrate optimization. (umn.edu)
  • In this study, we showed for the first time that RUNX2 is a novel substrate of AMP-activated kinase (AMPK), which directly phosphorylates at serine 118 residue in the DNA-binding domain of RUNX2. (uohyd.ac.in)
  • Site 2 was a good substrate for all three type‐2 phosphatases (2A, 2B and 2C) with t 0.5 values less than those toward the α subunit of phosphorylase kinase. (dundee.ac.uk)
Signal Transduction2
Assays2
  • As this interaction establishes a new link between the mitogenic cascade and protein biosynthesis, in vitro kinase assays were performed in presence of B- and C-RAF kinase and recombinant EF1A1/2 to investigate this important interaction. (unina.it)
  • Overall, this strategy represents a pipeline for developing efficient and specific assays for virtually any tyrosine kinase that use HTS-compatible lanthanide-based detection. (umn.edu)
Creatine kinase4
  • Expression of nucleoside diphosphate kinase, mitochondria-associated adenylate kinase, and several mitochondria-associated creatine kinase isozymes was highest in the outer retina, whereas expression of cytosolic adenylate kinase and brain creatine kinase was higher in the cones, horizontal cells, and amacrine cells indicating the diversity of ATP-buffering strategies among retinal neurons. (molvis.org)
  • Diagnosis depends on findings from patient history and physical examination, muscle biopsy, electromyography, ischemic forearm testing, and creatine kinase testing. (medscape.com)
  • A creatine kinase level evaluation is helpful in all cases of suspected glycogen storage disease (GSD). (medscape.com)
  • Serum creatine kinase levels (CK) can vary among healthy subjects, even when correcting for muscle mass. (medscape.com)
Oxidative phosphorylation3
Protein tyrosine kinase2
  • Subsequently, the change in protein tyrosine kinase activity was shown to underlie the Ras-MAPK signaling pathway regulated by mitogen-activated protein (MAP) kinases. (wikipedia.org)
  • and protein tyrosine kinase activity. (nih.gov)
CAMP-depend2
Subunit4
  • Phosphorylation and dephosphorylation of site 2 was rate‐limiting for dissociation and reassociation of C subunit. (dundee.ac.uk)
  • In conjunction with information presented in the following paper in this issue of this journal, the results substantiate the hypothesis that PP‐1 activity towards the glycogen‐metabolising enzymes is regulated in vivo by reversible phosphorylation of a targetting subunit (G) that directs the C subunit to glycogen-protein particles. (dundee.ac.uk)
  • Slight increases in the binding of a monoclonal antibody to the a subunit of CaM kinase II were seen, while large increases in the binding of [125Icalmodulin to the a subunit of CaM kinase II also were observed. (cdc.gov)
  • This enzyme also activates the transcription factor NF-KAPPA B and is composed of alpha and beta catalytic subunits, which are protein kinases and gamma, a regulatory subunit. (bvsalud.org)
Assay1
  • Peptide mixtures are arrayed in multiwell plates and analyzed by radiolabel assay with the kinase of interest. (networkin.info)
Specificity3
  • Violin plots show distribution of expression levels for Dual specificity tyrosine-phosphorylation-regulated kinase 2 (SMED30002728) in cells (dots) of each of the 12 neoblast clusters. (stowers.org)
  • Expression of Dual specificity tyrosine-phosphorylation-regulated kinase 2 (SMED30002728) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells. (stowers.org)
  • Violin plots show distribution of expression levels for Dual specificity tyrosine-phosphorylation-regulated kinase 2 (SMED30002728) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells. (stowers.org)
Pathway4
  • Since the kinetics of cofilin phospho-cycle was similar during secretion and platelet aggregation in LPA- and thrombin-stimulated cells, I propose a general two-step regulatory process for cofilin phospho-cycle underlying primarily secretion, and subsequently platelet aggregation: dephosphorylation by a cofilin phosphatase and then rephosphorylation by the Rho-kinase/LIMK-1 pathway. (uni-muenchen.de)
  • Tau tubulin kinase 2 (TTBK2) is a key player in the cilium assembly pathway, controlling the final step of cilia initiation. (muni.cz)
  • Another pathway of transmission of proliferative signals into the genome, with participation of growth factors and tyrosine kinases, is the monocascade STAT (signal transducer and activator of transcription) protein pathway activated by receptors of growth factors and cytokines. (wikipedia.org)
  • and transmembrane receptor protein tyrosine kinase signaling pathway. (nih.gov)
Residue2
  • Comparison of thermolytic phosphopeptide maps and determination of the phosphorylated residue by manual Edman degradation identified the main phosphorylation site in intact cells as Ser102. (cornell.edu)
  • In B cells isolated from PBMC of AD patients with high serum IgE levels, JAK3 was constitutively phosphorylated at the tyrosine residue, and its phosphorylation was enhanced by the treatment with CD40L and/or IL-4 as was that in splenic B cells of NC/Nga mice with dermatitis and high IgE levels. (johnshopkins.edu)
Peptides2
Pathways2
Phosphorylate1
  • In isolated enzyme systems, several kinases, including PKC and rho kinase (ROCK), have been shown to phosphorylate PHI-1. (uky.edu)
Cyclin-depende1
Enzymes1
Efficient phosphorylation1
  • Finally, we show that TTBK2 is also required for efficient phosphorylation of many S/T sites in CEP164 and provide evidence that TTBK2-induced phosphorylations of CEP164 modulate its function, which in turn seems relevant for the process of cilia formation. (muni.cz)
Vitro6
  • We found that Homer3, the predominant isoform in Purkinje cells, is phosphorylated by calcium/calmodulin-dependent protein kinase II (CaMKII) both in vitro and in vivo . (jneurosci.org)
  • An in vitro binding kinetic analysis revealed that these phosphorylation-dependent events were attributable to a decrease in the affinity of phosphorylated Homer3 for its ligand. (jneurosci.org)
  • Previously published data have shown an allele-specific variation in the in vitro binding of apolipoprotein E (apoE) to tau, which prompted the hypothesis that apoE binding may protect tau from phosphorylation, apoE3 being more efficient than apoE4. (ox.ac.uk)
  • We have, therefore, investigated the effects of apoE on tau phosphorylation in vitro by the proline-directed kinase, glycogen synthase kinase (GSK)-3 beta. (ox.ac.uk)
  • Our results in in vitro MSC lineage differentiation models confirmed that active AMPK and RUNX2-S118 phosphorylation are preferentially associated with osteogenic commitment, whereas the lack of this phosphorylation leads to adipogenesis. (uohyd.ac.in)
  • In vitro, DARPP-32 phosphorylated by casein kinase II was dephosphorylated by protein phosphatases-1 and -2A. (cornell.edu)
Family kinase2
  • A role of Src family kinase members in causing sterile inflammation and human disease is only recently recognized. (nature.com)
  • Regulation of MT dynamics via direct binding of an Abl family kinase. (nih.gov)
Vivo phosphorylation1
  • Owing to an increasing number of in vivo phosphorylation sites that have been identified by mass spectrometry (MS)-based proteomics, the RegPhos, available online at http://csb.cse.yzu.edu.tw/RegPhos2/, was developed to explore protein phosphorylation networks in human. (nih.gov)
Activity16
  • Immunoprecipitation and Western immunoblotting were employed to determine protein tyrosine phosphorylation, PI-3K activity and protein in lens cell extracts. (nih.gov)
  • The decrease in protein tyrosine phosphorylation correlated with the decrease in tyrosine kinase activity associated with the lens membrane fraction. (nih.gov)
  • The tyrosine kinase activity of insulin receptor isolated from the skeletal muscle of NIDDM patients has previously been found to be decreased compared with the activity of receptor from nondiabetic subjects but the mechanism underlying this defect is unknown. (jci.org)
  • Moreover, within each group there was no correlation between the level of tyrosine kinase activity and the extent of serine/threonine phosphorylation. (jci.org)
  • It is concluded that the stoichiometry of serine/threonine phosphorylation of insulin receptor in vivo is low, and that increased phosphorylation of Ser 1327 or Thr 1348 is not responsible for the decreased insulin receptor tyrosine kinase activity observed in the skeletal muscle of NIDDM patients. (jci.org)
  • Together, these findings suggested that Homer3 in Purkinje cells might function as a reversible coupler regulated by CaMKII phosphorylation and that the phosphorylation is capable of regulating the postsynaptic molecular architecture in response to synaptic activity. (jneurosci.org)
  • These results suggest that the phosphorylation may regulate postsynaptic molecular architecture and Ca 2+ signaling of Purkinje cells in response to synaptic activity. (jneurosci.org)
  • The effect of phosphorylation by cyclic GMP-dependent protein kinase (G-kinase) on the activity of the plasmalemmal Ca2+-transport ATPase was studied on isolated plasma membranes and on the ATPase purified from pig erythrocytes and from the smooth muscle of pig stomach and pig aorta. (portlandpress.com)
  • We characterize the role of increased Lyn kinase activity in neutrophils, endothelial cells and lesional liver biopsies and utilized an iPSC-derived endothelial cell platform for disease modeling of neutrophilic vasculitis and to screen and evaluate drug efficacy. (nature.com)
  • This modification was dependent on extracellular signal-regulated kinase (ERK) activity triggered by HSV infection. (bris.ac.uk)
  • Integrin-mediated RON growth factor receptor phosphorylation requires tyrosine kinase activity of both the receptor and c-Src. (123dok.org)
  • on the kinase activity of both RON itself and c-Src. (123dok.org)
  • Integrin-in-duced epidermal growth factor (EGF) and platelet-derived growth factor (PDGF) RTK phosphorylation depends on the kinase activity of the receptor (7, 13). (123dok.org)
  • The potential functional consequences of PKC beta-induced retinoblastoma phosphorylation could include enhanced E2 promoter binding factor transcriptional activity and increased VEGF -induced endothelial cell proliferation. (bvsalud.org)
  • At physiological ionic strength, phosphorylation of glycogen‐bound PP‐1 G was found to release all the phosphatase activity from glycogen. (dundee.ac.uk)
  • These observations suggest a mechanism whereby CDK phosphorylation has the potential to down regulate eIF4A activity and thereby affect translation. (aber.ac.uk)
Regulate2
  • The small GTPase Rho can regulate several aspects of cellular function, predominantly through its downstream effector Rho-kinase. (uni-muenchen.de)
  • We have a specific focus on protein kinases that regulate adipose tissue function, since defects in this tissue, for example in its ability to efficiently store fat, is an underlying cause of insulin resistance and diabetes. (lu.se)
Cdk14
  • Targets of the cyclin-dependent kinase Cdk1. (nature.com)
  • NDEL1 preferentially localizes to the centrosome and is a likely target for cell cycle-activated kinases, including CDK1. (elsevierpure.com)
  • In particular, NDEL1 phosphorylation by CDK1 facilitates katanin p60 recruitment to the centrosome and triggers microtubule remodeling. (elsevierpure.com)
  • Previously, we found that eukaryotic initiation factor (eIF) 4A interacts with cyclin dependent kinase A (CDKA), the plant ortholog of mammalian CDK1. (aber.ac.uk)
Myosin1
Glycolysis1
  • The combined results indicate that glycolysis is regulated by the compartmental expression of hexokinase 2, pyruvate kinase M1, and pyruvate kinase M2 in photoreceptors, whereas the inner retinal neurons exhibit a lower capacity for glycolysis and aerobic glycolysis. (molvis.org)
Intracellular2
  • Protein phosphorylation catalyzed by kinases plays crucial roles in regulating a variety of intracellular processes. (nih.gov)
  • In 1990 receptor tyrosine kinase (RTK) initiation of intracellular signaling was detected. (wikipedia.org)
Biochemical1
  • Here, we propose a molecular mechanism where the AQP7 mobility in adipocytes is dependent on perilipin 1 and protein kinase A. Biochemical analyses combined with ex vivo studies in human primary adipocytes, demonstrate that perilipin 1 binds to AQP7, and that catecholamine activated protein kinase A phosphorylates the N-terminus of AQP7, thereby reducing complex formation. (lu.se)
Extracellular1
  • Olsson PO, Gustafsson R, In 't Zandt R, Friman T, Maccarana M, Tykesson E, Oldberg Å, Rubin K, Kalamajski S. The Tyrosine Kinase Inhibitor Imatinib Augments Extracellular Fluid Exchange and Reduces Average Collagen Fibril Diameter in Experimental Carcinoma. (lu.se)
Metabolism1
  • An overall aim of our research is to study the regulation and function of protein phosphorylation cascades that are important for the maintenance of a normal energy metabolism and that might be involved in the pathophysiological changes that ultimately lead to type 2 diabetes. (lu.se)
Actin1
  • Our results showing that only dephosphorylated (activated) cofilin binds with F-actin support previous observations that the state of cofilin phosphorylation determines its association with F-actin. (uni-muenchen.de)
Stimulation3
Enzyme1
  • CK, formerly known as creatinine phosphokinase, is an important protein enzyme catalyzing the reversible phosphorylation reaction. (medscape.com)
Glycogen1
  • Differential effects of apolipoprotein E isoforms on phosphorylation at specific sites on tau by glycogen synthase kinase-3 beta identified by nano-electrospray mass spectrometry. (ox.ac.uk)
Facilitates1
Mass spectrometry1
  • Mass spectrometry analysis identified treonine 88 (exclusively mediated by B-RAF) and serine 21 on EF1A1 and serine 21 on EF1A2 as B-/ and C-RAF mediated phosphorylation sites. (unina.it)
Cellular3
  • Cellular ATP is buffered by specialized equilibrium-driven high-energy phosphate (~P) transferring kinases. (molvis.org)
  • Protein kinases are ubiquitous components of cellular signalling networks [ 1 ]. (biomedcentral.com)
  • Nonreceptor protein tyrosine kinases (NRTKs) are essential for cellular homeostasis and thus are a major focus of current drug discovery efforts. (umn.edu)
Cells8
  • Furthermore, both robust phosphorylation of Homer3 and its dissociation from metabotropic glutamate receptor 1α (mGluR1α) were triggered by depolarization in primary cultured Purkinje cells, and these events were inhibited by CaMKII inhibitor. (jneurosci.org)
  • In this study, we revealed that Homer3 was phosphorylated by calcium/calmodulin-dependent protein kinase II (CaMKII) in Purkinje cells, and the phosphorylation reduces the affinity for Homer target molecules, resulting in change of the solubility. (jneurosci.org)
  • It is proposed that G-kinase stimulates the plasmalemmal Ca2+ pump of smooth-muscle cells indirectly via the phosphorylation of an associated PI kinase. (portlandpress.com)
  • Previous experiments in H1355 cells demonstrated that IFN treatment results in an antiapoptotical answer during which EF1A2 is increasingly expressed due to its RAF kinase mediated phosphorylation. (unina.it)
  • The expression of Aurora-A phosphorylation-mimetic mutants of NDEL1 efficiently rescued the defects of centrosomal maturation and separation which are characteristic of Aurora-A-depleted cells. (elsevierpure.com)
  • Phosphorylation of DARPP-32 by casein kinase II in intact cells may therefore modulate its phosphorylation in response to increased levels of cAMP. (cornell.edu)
  • Association of PKC beta 2 isoform with retinoblastoma protein was discovered in retinal endothelial cells , and PKC beta 2 isoform increased retinoblastoma phosphorylation under basal and VEGF -stimulated conditions. (bvsalud.org)
  • Thus, it is suggested that constitutive and enhanced JAK3 phosphorylation in B cells highly sensitive to CD40L and IL-4 may be attributable to IgE hyperproduction in NC/Nga mice and patients with AD. (johnshopkins.edu)
Signaling1
Sequence1