Phosphorylate biological systems map kinase signaling system. Medical search. Frequent questions

This study was designed to identify signaling pathways and crucial proteins involved in the pathological mechanism of KBD compared with osteoarthritis (OA). (nature.com)
KEGG pathways enrichment and PPI network indicate four major pathways, including extracellular matrix -receptor interaction, focal adhesion, phosphatidylinositol 3-kinase (PI3K)-Protein kinase B (Akt), and Ras signaling pathways were involved in the degeneration of cartilage. (nature.com)
In conclusion, our results demonstrated that compared with OA, the differential abundance proteins and signaling pathways may contribute to the occurrence and development of joint damage in KBD. (nature.com)
Mitogen-activated protein (MAP) kinase pathways include a three-kinase cascade terminating in a MAP kinase family member. (elsevierpure.com)
posttranslational modifications, amino acid variations, computational mutation analysis, protein PTM predictor, network biology Introduction Protein PTMs are biochemical alterations of amino acids that change the physicochemical properties of target proteins, leading to structural changes and therefore regulating protein-protein interactions and cellular signal transduction in developmental and cancer pathways [1]. (deepdyve.com)
Western blotting of brain lysates and an in vitro neurotoxicity assay were employed for analysis of cellular signaling pathways. (biomedcentral.com)
The induction of ATF-3 expression was mediated by mitogen-activated protein kinase (MAPK) signaling pathways in GS-002-treated cells. (hindawi.com)
The potential signaling pathways for RIL were identified and experimentally validated using gene ontology (GO) enrichment and Kyoto encyclopedia of genes and genomes (KEGG) analysis. (frontiersin.org)
Several anterograde and retrograde signaling pathways, including the canonical Bone Morphogenic Protein (BMP) pathway , regulate synaptic development in vertebrates and invertebrates. (sdbonline.org)
Next, we mapped the altered genes using the Kyoto Encyclopedia of Genes and Genomes (KEGG) PATHWAY database, and investigated several pathways that may be affected by IHT. (biomedcentral.com)
The current research aims to identify shared molecular gene signatures and pathways that affirm the epidemiological association of T2DM and TB and afford clues on mechanistic basis of their association through integrative systems biology and bioinformatics approaches. (springeropen.com)
Furthermore, these two genes are modulators in clusters C4 and C5, abundant in cytokine/chemokine signalling and Jak-STAT signalling pathways. (springeropen.com)
In addition, the ErbB receptors integrate a diverse array of signals, both at the cell surface level and through cross-talk with other pathways, such as the phosphoinositide 3-kinase (Pi3k) pathway [ 11 ]. (biomedcentral.com)
Peritoneal stomata constitute the principal pathways for the drainage of intraperitoneal contents from the PERITONEAL CAVITY to the LYMPHATIC SYSTEM. (bvsalud.org)
Plants are immobile and, to overcome harsh environmental conditions such as drought, salt, and cold, they have evolved complex signaling pathways. (mdpi.com)

p38α MAPK is mainly activated through MAPK kinase kinase cascades and exerts its biological function via downstream substrate phosphorylation. (wikipedia.org)
The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. (wikipedia.org)
Like all MAP kinases, p38α MAPK has 11 conserved domains (Domains I to XI) and a Thr-Gly-Tyr (TGY) dual phosphorylation motif. (wikipedia.org)
MAP2K mediated phosphorylation of the TGY motif results in conformational change of p38 MAPK which allows kinase activation and accessibility to substrates. (wikipedia.org)
To map the mechanisms of aggressiveness we aimed to investigate changes in protein phosphorylation across WHO grades of human meningiomas. (nature.com)
Reconstitution of mitogen-activated protein kinase phosphorylation cascades in bacteria. (elsevierpure.com)
Dive into the research topics of 'Reconstitution of mitogen-activated protein kinase phosphorylation cascades in bacteria. (elsevierpure.com)
Phosphorylation of PINK1 is not only required for the PINK1-mediated mitochondrial recruitment of Parkin but also induces its kinase activity toward Parkin. (sdbonline.org)
Intersecting analysis, molecular docking, and pathway validation analysis showed that risperidone influences the adipocytokine signaling pathway by targeting MAPK14 (mitogen-activated protein kinase 14), MAPK8 (mitogen-activated protein kinase 8), and RXRA (retinoic acid receptor RXR-alpha), thereby inhibiting long-chain fatty acid β-oxidation by decreasing STAT3 (signal transducer and activator of transcription 3) expression and phosphorylation. (frontiersin.org)
Schip1 is necessary for promoting membrane localization and phosphorylation of Hpo by recruiting the Hpo kinase Tao-1 . (sdbonline.org)

MAPK14 encodes p38α mitogen-activated protein kinase (MAPK) which is the prototypic member of the p38 MAPK family. (wikipedia.org)
p38α MAPK was originally identified as a tyrosine phosphorylated protein detected in activated immune cell macrophages with an essential role in inflammatory cytokine induction, such as Tumor Necrotic Factor α (TNFα). (wikipedia.org)
However, p38α MAPK mediated kinase activity has been implicated in many tissues beyond immune systems. (wikipedia.org)
p38α MAPK is implicated in diverse cellular functions, from gene expression to programmed cell death through a network of signaling molecules and transcription factors. (wikipedia.org)
Pharmacological and genetic inhibition of p38α MAPK not only revealed its biological significance in physiological function but also the potential of targeting p38α MAPK in human disease such as immune disorders and heart failure. (wikipedia.org)
p38α MAPK is ubiquitously expressed in many cell types, in contrast, p38β MAPK is highly expressed in brain and lung, p38γ MAPK mostly in skeletal muscle and nerve system, and p38δ MAPK in uterus and pancreas. (wikipedia.org)
In addition, one substrate of p38 MAPK, MAP kinase-activated protein kinase 2 (MAPAK2 or MK2) can modulate and direct p38α MAPK localization to cytosole via direct interaction. (wikipedia.org)
In vitro, inhibition of p38 MAPK abrogates neurotoxicity caused similarly by blockade of ERK kinase and HIVgp120. (biomedcentral.com)
Deregulation in signaling along the EgfR-MAPK pathway is common in breast cancer, though the manner in which deregulation occurs varies between both individuals and cancer subtypes. (biomedcentral.com)
To that end, we mapped genomic, transcriptional and proteomic profiles for 30 breast cancer cell lines onto a curated Pathway Logic symbolic systems model of EgfR-MAPK signaling. (biomedcentral.com)
The mitogen-activated protein kinase (MAPK)/Erk pathway plays a central role in cell communication: it orchestrates signaling from external receptors to internal transcriptional machinery, which leads to changes in phenotype [ 6 , 7 ]. (biomedcentral.com)
Activation of MAPK is initiated by one of the four ErbB receptors (ErbB1/epidermal growth factor receptor (EgfR), ErbB2-4), which leads to signaling through Raf (RAF proto-oncogene serine/threonine-protein kinase), Mek (mitogen-activated protein kinase kinase 1/2) and Erk. (biomedcentral.com)
We are currently pursuing studies of the dominant signaling and gene regulatory networks that control this process, including the Ras/MAPK cascade, which is required for stem cell-mediated self-renewal and the p53 transcription factor family member, p63, which is required for epidermal differentiation. (stanford.edu)

The Parkinson's disease genes pink1 and parkin , which encode a mitochondrially targeted protein kinase, and an E3 ubiquitin ligase, respectively, participate in a key mitochondrial quality-control pathway that eliminates damaged mitochondria. (sdbonline.org)
The anchor cell induces nearby epidermal precursor cells to generate vulval cells via an epidermal growth factor (EGF) signaling pathway. (wormbook.org)
In vitro , GnRH neuronal cell lines respond to a variety of ligands that activate the Jak (Janus-activated kinase)/STAT (signal transducers and activators of transcription) intracellular signaling pathway. (jneurosci.org)
This signaling pathway is a highly conserved cell-to-cell communication system that regulates cell fate in development and disease. (5dok.org)
Eph/ephrin signaling is a cell-to-cell communication pathway, which regulates cell migration and proliferation. (5dok.org)
In Drosophila, Hpo and Warts kinases are core components of this pathway and function as tumor suppressors by inhibiting Yorkie (Yki) . (sdbonline.org)
Neuronal loss of the serine-threonine protein kinase Tao , a regulator of the Hippo signaling pathway, results in supernumerary boutons, each of which contain a normal number of active zones. (sdbonline.org)
In addition, IHT increased phosphorylated p70 S6 kinase, indicating involvement of the mammalian target of rapamycin signaling pathway. (biomedcentral.com)
The functional enrichment and gene interaction network analysis emphasized the importance of cytokine/chemokine signalling, T cell receptor signalling route, NF-kappa B signalling pathway and Jak-STAT signalling system. (springeropen.com)
We applied functional pathway analysis to show that HPV-positive cancers are characterised by perturbations of numerous cellular processes, predominantly in those linked to the cell cycle, mitosis, cytokine and immune cell signalling. (fortuneonline.org)

Mitogen-activated protein kinase 14, also called p38-α, is an enzyme that in humans is encoded by the MAPK14 gene. (wikipedia.org)
The protein encoded by this gene is a member of the MAP kinase family. (wikipedia.org)
GS-002 also induced endoplasmic reticular (ER) stress as evidenced by increases in ER stress-responsive proteins including glucose-regulated protein 78 (GRP78), growth arrest- and DNA damage-inducible gene 153 (GADD153), phosphorylated eukaryotic initiation factor 2 α (eIF2 α ), phosphorylated protein endoplasmic-reticular-resident kinase (PERK), and ATF-3. (hindawi.com)
Instead, the experiments suggest that Tao negatively regulates BMP signaling as reduction of Tao leads to an increase in pMad levels in motor neuron nuclei and an increase in BMP target gene expression. (sdbonline.org)
Consistent with the gene analyses, in vivo analyses revealed that IHT increased phosphorylated tau, reflecting an imbalance of kinases and/or phosphatases, and reduced proteins relevant to glutamatergic synapses. (biomedcentral.com)
Impaired insulin signalling, glucose and lipid homeostasis alter the gene signalling profile in both organs and serum during prediabetic and diabetic conditions [ 9 ]. (springeropen.com)
The present invention provides a simple and robust human liver cell-based system in which persistent hepatitis C infection, persistent hepatitis B infection or ethanol exposure induces a clinical Prognostic Liver Signature (PLS) high-risk gene signature. (sumobrain.com)

EGF-receptor and Notch signaling are antagonistic: EGF-receptor signaling leads to down-regulation of the Notch-like receptor LIN-12 , while LIN-12 signaling induces negative regulators of EGF-receptor signaling such as MAP kinase phosphatase LIP-1 and the tyrosine kinase ARK-1. (wormbook.org)
Jak2 belongs to the nonreceptor tyrosine kinase family ( Ihle, 1995 ). (jneurosci.org)

The largest family of cell surface receptors involved in SIGNAL TRANSDUCTION. (lookformedical.com)
A network of intercellular signaling, signal transduction, and transcriptional regulation underlies the precise formation of this organ, which is the connection between the hermaphrodite uterus and the outside of the nematode. (wormbook.org)
Mathematical modeling of signal transduction networks has previously been used to map out thermodynamical using rate equations is increasingly attracting attention as a properties of protein-folding models (6,7). (lu.se)

In the discovery set of fresh-frozen tissue specimens (14), the A-kinase anchor protein 12 (AKAP12) protein was found downregulated across the grades. (nature.com)
Likewise, activated p38 and α-stress-activated protein kinase could also be inactivated by either phosphatase, and α-stress- activated protein kinase was recognized by an antibody specific to the doubly phosphorylated forms. (elsevierpure.com)
Coexpression of protein kinases with their substrates in bacteria is of great value in the preparation of numerous phosphoproteins, heretofore not possible in procaryotic expression systems. (elsevierpure.com)
The technology has been shown to be applicable to all major classes of drug targets, including GPCRs, kinases, enzymes, ion channels, and protein-protein and protein-ligand interactions, enabling broader research applicability. (drugdiscoverynews.com)
In this regard, comprehensive studies of the impact of amino acid variation on protein PTMs will be helpful for further understanding of how genetic polymorphisms are involved in regulating biological and pathological processes and providing instructive information for drug development of various related diseases. (deepdyve.com)
Regulatory proteins that down-regulate phosphorylated G-protein membrane receptors, including rod and cone photoreceptors and adrenergic receptors. (lookformedical.com)
Arrestin quenches G-protein activation by binding to phosphorylated photolyzed rhodopsin. (lookformedical.com)
A family of serine-threonine kinases that are specific for G-PROTEIN-COUPLED RECEPTORS. (lookformedical.com)
A ubiquitously expressed G-protein-coupled receptor kinase subtype that has specificity for the agonist-occupied form of BETA-ADRENERGIC RECEPTORS and a variety of other G-PROTEIN-COUPLED RECEPTORS. (lookformedical.com)
Although it is highly homologous to G-PROTEIN-COUPLED RECEPTOR KINASE 2, it is not considered to play an essential role in regulating myocardial contractile response. (lookformedical.com)
A G-protein-coupled receptor kinase subtype that is primarily expressed in the MYOCARDIUM and may play a role in the regulation of cardiac functions. (lookformedical.com)
This sheds light upon the role of the Smad7 protein in this system. (lu.se)

BiComp-DTA utilizes BiComp measure to provide a way for the area, we will utilize the proposed unified measure based on the recycling of spent lithium-ion batteries (LIBs) by the kinase dissociation constant (Kd), for 68 kinase inhibitor selectivity. (soisdetraca.com)
Previous studies reported that ATF-3 was induced by treating cells with antitumorigenic compounds [ 14 - 18 ] and a phosphoinositide 3-kinase inhibitor [ 19 ]. (hindawi.com)
Taken together, these results support a role for Tao as a novel inhibitor of BMP signaling in motor neurons during synaptic development and function. (sdbonline.org)

Hematopoietic PTP (HePTP) and striatal-enriched phosphatase (STEP) bind to MAPKs through a kinase-interaction motif (KIM) and inactivates them by dephosphorylating the phosphotyrosine residue in their activation loop. (wikipedia.org)
Consistent with their high activity, ERK1 and ERK2 were doubly phosphorylated on Tyr and Thr, were recognized by an antibody specific to the doubly phosphorylated forms, and were inactivated by either phosphoprotein phosphatase 2A or phosphotyrosine phosphatase type 1. (elsevierpure.com)

Eye proteins are the biological molecules that make up the various structures of the eye and are essential for its proper function. (lookformedical.com)
Oligomerization is a frequently encountered physical characteristic of biological molecules that occurs for a wide number of transcription factors, ion channels, oxygen-carrying macromolecules such as hemocyanin and enzymes. (baumaschinen-knell.de)
The CNS response is thought to be mediated, at least in part, through intermediate signaling molecules that affect GnRH neuronal activity. (jneurosci.org)
The cytokine receptors lack intrinsic kinase activity and require interaction with members of the Jak family of intracellular signaling molecules. (jneurosci.org)

Since HIV-1 activates the type I interferon system, which signals via interferon-α receptor (IFNAR) 1 and 2, this study investigated the potential role of IFNAR1 in HIV-induced neurotoxicity. (biomedcentral.com)
Receptor activation causes Jak2 autophosphorylation, which phosphorylates its associated receptor. (jneurosci.org)
The phosphorylated receptor provides multiple docking sites that recruit STAT proteins, of which there are six family members (STAT1-6) ( Pellegrini and Dusanter-Fourt, 1997 ). (jneurosci.org)

A c-jun amino-terminal kinase that is found predominantly within NEURONS of the BRAIN, suggesting a role in stress-induced neuronal APOPTOSIS. (lookformedical.com)
Specify the components and their interactions and set up cluding proliferation, differentiation, apoptosis, embryonic the system of equations. (lu.se)

The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress-related transcription and cell cycle regulation, as well as in genotoxic stress response. (wikipedia.org)
We split trials in Experiment 1 suggested that CSF flow in which an outbreak by use of various substrates to produce a predicted CSF signal, we took the negative derivative of the epidemic. (soisdetraca.com)

Gonadotropin-releasing hormone (GnRH) neurons represent the final common output of signals from the brain that regulates reproductive function. (jneurosci.org)

A two-plasmid bacterial expression system was employed to express active forms of the following MEK and MAP kinase family members: ERK1, ERK2, α-SAPK, and p38 and their upstream activators, MEK1, -2, -3, and -4. (elsevierpure.com)
In each kinase module, the upstream activator, a constitutively active mutant of MEK1 or MEKK1, was expressed from a low copy plasmid, while one or two downstream effector kinases were expressed from a high copy plasmid with different antibiotic resistance genes and origins of replication. (elsevierpure.com)

GO analysis shows that the differential abundant proteins are associated with cell junction and signal transducer activity from extracellular to intracellular. (nature.com)
The middle kinase in the cascade is a MAP/extracellular signal.regulated kinase (ERK) kinase or MEK family member and is highly specific for its MAP kinase target. (elsevierpure.com)

And most recently, last month, we reported that Activiomics and Belgian biopharma UCB will apply Activiomics' Targeted In-depth Quantification of cell Signaling (TIQUAS) phospho-proteomics platform-a system that is quantitative, label-free and applicable to cell and tissue samples-in a collaborative effort to elucidate signaling mechanisms of therapeutic antibodies in relevant cell-based systems. (drugdiscoverynews.com)
These results strongly circumscribe the possible mechanisms of PINK1 action in the mitochondrial life cycle and also raise the possibility that mitochondrial turnover events that occur in cultured embryonic axons might be restricted to the cell body in vivo, in the intact nervous system. (sdbonline.org)
While much is known about biochemical signaling, many of the mechanisms that drive cell outcome in response to biophysical influences remain to be uncovered. (5dok.org)
This analysis discriminates between different biological mechanisms to achieve a transient signal from a sustained TGF-b input, where one mechanism is to use a negative feedback to turn the signal off. (lu.se)

We obtained important data or something from the massive amount of microarray data, and confirmed the validity by in vivo analyses: the IHT-induced cognitive impairment may be partially explained by the fact that IHT increases phosphorylated tau via biological processes common to aging. (biomedcentral.com)
Finally, we confirmed the insights obtained from in silico analyses by performing in vivo analyses, with findings indicating that IHT increases level of phosphorylated tau (P-tau). (biomedcentral.com)

MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. (wikipedia.org)

The precise pattern of vulval precursor cell fates involves the graded action of the EGF signaling and LIN-12 (Notch) mediated lateral signaling. (wormbook.org)
see RTKRas/MAP kinase signaling ), LIN-12 (see LIN-12/Notch signaling in C. elegans ), and WNT (see Wnt signaling ), as well as the functions of the SynMuv and mediator proteins (see Transcriptional regulation ). (wormbook.org)
We have therefore investigated the role of matrix stiffness in Notch signaling in breast cancer cells. (5dok.org)
Aberrant Notch signaling in breast cancer has been found to correlate with invasion and patient outcome. (5dok.org)

The reversed polarity of the posterior 2 ° precursor cell lineage requires WNT signaling mediated by both Frizzled class and Ryk class WNT-receptors LIN-17 and LIN-18 , respectively. (wormbook.org)

renders six epidermal precursor cells competent to respond to other developmental signals. (wormbook.org)
Further analysis in terms of parameter sensitivity reveals that this negative feedback loop in TGF-b signaling renders the system global robustness. (lu.se)

The cellular model system for hepatocellular carcinoma (HCC)/cirrhosis development and progression may be used in the screening of compounds useful in the treatment and/or prevention of cirrhosis and/or HCC as well as in the identification biomarkers for the prediction of liver disease (especially cirrhosis) progression and HCC. (sumobrain.com)

Phosphoproteomic techniques have been widely employed to identify kinase activity, which may lend itself to pharmaceutical blockade. (nature.com)

In November 2010, we brought you the news that ACEA Biosciences Inc. and Vivo Biosciences Inc. agreed to work together to develop label-free and real-time cell-based assays for the xCELLigence System, which is co-developed by Roche and ACEA and marketed by Roche Applied Science. (drugdiscoverynews.com)
This study evaluated the roles of PINK1 mitochondrial kinase, and Parkin E3 ubiquitin ligase in targeting depolarized mitochondria for degradation in vivo, using quantitative measurements of mitochondria in Drosophila nervous system. (sdbonline.org)

The conserved Ste20-like kinase Tao was identified as a negative regulator of synaptic growth required for maintenance of circuit specificity and connectivity. (sdbonline.org)

The first kinase in the cascade, a MEK kinase (MEKK), is characterized by its ability to activate one or more MEK family members. (elsevierpure.com)

among others, CDK1, CDK2 and CDK4, as the key kinases that control the biological processes in HPV-positive cervical cancers. (fortuneonline.org)

These results demonstrate an essential role for Jak2 signaling in GnRH neurons for normal reproductive development and fertility in female mice. (jneurosci.org)

HIV primarily infects CD4 + cells of the immune system, such CD4 + T cells and monocytes/macrophages and microglia in the CNS [ 8 ]. (biomedcentral.com)

Taking these findings together, it is concluded that Schip1 directly links Ex to Hpo signaling by recruiting Tao-1. (sdbonline.org)

All applications in biotherapeutic discovery and development such as epitope mapping and binning and antibody screening are impossible, or at least highly cumbersome, with labeled techniques. (drugdiscoverynews.com)

Journal of Bioinformatics and Systems Biology 4 (2021): 33-49. (fortuneonline.org)

Label-free detection, on the other hand, is proving to be a highly sensitive method of measurement for endogenous targets in live cell assays, and eliminates the need for tags, dyes or specialized reagents or engineered cells-reducing the resources required for assay development, simplifying assay design, minimizing liabilities created by the use of labels and enabling the use of native cells for greater biological relevance. (drugdiscoverynews.com)
the pattern of vulE and vulF cells requires an additional signal from the anchor cell as well as WNT signaling. (wormbook.org)
Cells constantly receive and translate physical cues into biological messages, which in turn dictate cell shape, state and function. (5dok.org)

All together, our results support the utility of symbolic system biology models for identification of therapeutic approaches that will be effective against breast cancer subsets. (biomedcentral.com)

and report here a role for the Jak2 signaling molecule in the regulation of reproductive development and function in female mice. (jneurosci.org)
Regulation of organ size is essential in animal development, and Hippo (Hpo) signaling is a major conserved mechanism for controlling organ growth. (sdbonline.org)

responsible for many different biological functions, in- 1. (lu.se)
Here, I present results from different biological projects where SANS has played a crucial role by providing unique restraints for structural refinement and interpretation, complementary to other techniques (NMR, EM, crystallography). (lu.se)

Welcome Family is a universal, short-term nurse home visiting program designed to promote optimal maternal and infant physical and mental well-being and provide an entry point into the early childhood system of care to all families with newborns up to 8 weeks old living in defined communities in Massachusetts. (cdc.gov)

To determine its biological function in reproduction, we used Cre (cAMP response element)/LoxP technology to generate GnRH neuron-specific Jak2 conditional knock-out (Jak2 G −/− ) mice. (jneurosci.org)
The systems tend to have many para- about its dynamics and function. (lu.se)

Anatomy16

Organisms8

Chemicals and Drugs141

Analytical, Diagnostic and Therapeutic Techniques and Equipment23

Phenomena and Processes55

Disciplines and Occupations5

Technology, Industry, Agriculture2

Information Science7

Health Care3

Pathways15

Phosphorylation10

MAPK13

Pathway10

Gene7

Tyrosine kinase2

Transduction3

Protein12

Inhibitor3

Phosphatase2

Molecules4

Receptor3

Apoptosis2

Substrates2

Regulates1

Upstream2

Extracellular2

Mechanisms4

Analyses2

Biochemical1

Notch4

RECEPTORS1

Renders2

Cellular1

Blockade1

Vivo2

Specificity1

Cascade1

Processes1

NEURONS1

Immune1

Findings1

Antibody1

Bioinformatics1

Cell3

Therapeutic1

Regulation2

Different biological2

Family1

Function2