PolyclonalImmunogenPurificationSPECIFICITYChromatographySyntheticEpitope specific phosphopeptideAntigenSynthesized phosphopeptide derivedProteinProteinsDetects endogenous levelDatasheetResiduesIsotypeCalf intestinal phosphataseMonoclonal antibodiesPhosphataseSecondary AntibodyPeptidesCustom antibodyImmunizationRabbitsGlycerolImmunoprecipitationSer473LysatePhosphorylation site of serineAliquotUbiquitinReceptorRecombinantHumanPeptide synthesisSubstratePrimaryBlotMembraneExtractsGenesVialAssayTranscription
Polyclonal9
- The following product was used in this experiment: Phospho-CaMKII alpha/beta/delta (Tyr231) Polyclonal Antibody from Thermo Fisher Scientific, catalog # PA5-17108, RRID AB_10987028. (thermofisher.com)
- NeoScientific's standard option for polyclonal antibody is optimized to produce antiserum in the shortest possible time. (neobiolab.com)
- The following product was used in this experiment: Phospho-Syk (Tyr323, Tyr317) Polyclonal Antibody from Thermo Fisher Scientific, catalog # 44-234G, RRID AB_2533612. (thermofisher.cn)
- Traditional polyclonal and monoclonal antibodies are the product of normal B cell development and genetic recombination. (cellsignal.jp)
- While polyclonal antibodies are secreted by many different B cell clones and recognize multiple antigenic epitopes, monoclonals originate from a single B cell clone and are specific for just one epitope. (cellsignal.jp)
- Where polyclonal antibodies are purified directly from the serum of the immunized host, and monoclonals are purified from either hybridoma-derived tissue culture supernatant or ascites, recombinant antibodies are instead purified from the tissue culture supernatants of transfected host cell lines. (cellsignal.jp)
- Regardless of whether an antibody is polyclonal, monoclonal or recombinant, it must always be properly validated in the intended application prior to experimental use. (cellsignal.jp)
- Our Anti-Interferon-α Receptor, Type I, Subunit I (Ser535,539) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. (phosphosolutions.com)
- Our Anti-Potassium Chloride Cotransporter (KCC2) (Ser940) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. (aveslabs.com)
Immunogen2
- Antibodies directed against immunogen-coupled phosphorylated PEPTIDES corresponding to amino acids surrounding the PHOSPHORYLATION site. (jefferson.edu)
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (origene.com)
Purification6
- Therefore, the current and simplest methods to enrich phosphoproteins are affinity purification using phosphospecific antibodies, immobilized metal affinity chromatography (IMAC), strong cation exchange (SCX) chromatography, or titanium dioxide chromatography. (wikipedia.org)
- Antiphosphotyrosine antibodies have been proven very successful in purification, but fewer reports have been published using antibodies against phosphoserine- or phosphothreonine-containing proteins. (wikipedia.org)
- This antibody is purified through a protein A column, followed by peptide affinity purification. (avivasysbio.com)
- Althought most projects can be completed within 3-6 months, actual processing time is dependent on the peptide synthesis, animal immunization processes and antibody purification. (abbiotec.com)
- Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns. (phosphosolutions.com)
- However, attempts to hire these reagents as antibody substitutes within an immunoaffinity purification-like program showed that they don't perform aswell as real antibodies which significant marketing will be needed. (euromedh2020.com)
SPECIFICITY4
- Specificity This antibody detects endogenous human, mouse, and rat GATA1 only when phosphorylated at serine 310. (qedbio.com)
- We have extensive experience developing high-specificity phospho-specific antibodies for our customers. (neobiolab.com)
- At CST, we adhere to the Hallmarks of Antibody Validation™ , six complementary strategies for determining the specificity, sensitivity, and functionality of an antibody in any given assay. (cellsignal.jp)
- The specificity of this antibody refers to P10636-8. (abcam.cn)
Chromatography5
- This is due to the optimization of enrichment protocols for phosphoproteins and phosphopeptides, better fractionation techniques using chromatography, and improvement of methods to selectively visualize phosphorylated residues using mass spectrometry. (wikipedia.org)
- The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. (abcam.com)
- Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. (prosci-inc.com)
- ab39402 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. (abcam.cn)
- Purified from rabbit serum by sequential epitope-specific chromatography. (abcam.cn)
Synthetic6
- Synthetic peptide corresponding to Human Histone H3 (phospho S10). (abcam.com)
- This MEF2C Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S387 of human MEF2C. (avivasysbio.com)
- Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. (arigobio.cn)
- Synthetic peptide corresponding to Human ASK1 aa 950-1050 (phospho S966). (abcam.cn)
- Synthetic peptide corresponding to Human Tau (phospho S199). (abcam.cn)
- Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser535/539 of human interferon-α, conjugated to keyhole limpet hemocyanin (KLH). (phosphosolutions.com)
Epitope specific phosphopeptide1
- The antibody was purified using epitope specific phosphopeptide. (abcam.com)
Antigen11
- When the antibody for a chosen antigen is not already available off the shelf, it is time to develop a custom project. (abbiotec.com)
- The experienced staff of Abbiotec processes a custom project with the same mindset and tools that it develops antibody products for the Abbiotec catalog: we want an antibody that specifically detects the antigen in the chosen application. (abbiotec.com)
- With 3 mg of protein as antigen, 100-200 mg of purified antibody is generated in rabbits against multiple epitopes within 3 months. (abbiotec.com)
- A peptide encompassing a short region of the target protein is used as antigen to generate in rabbits 100-200 mg of purified antibody within 4 months. (abbiotec.com)
- For monitoring target activation by phosphorylation, a phosphopeptide is used as antigen to generate in rabbits 5-10 mg of affinity-purified antibody within 4 months. (abbiotec.com)
- Abbiotec guarantees that the antibody generated using an antigen synthesized by Abbiotec will recognize the antigen in ELISA. (abbiotec.com)
- This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. (thermofisher.cn)
- Syk translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. (thermofisher.cn)
- The specific immunolabeling is blocked by the phosphopeptide (+) used as the antigen in the second blot. (phosphosolutions.com)
- Furthermore, it could be the case the fact that resultant antibodies usually do not work very well in immunoprecipitation tests due to imperfect exposure from the antigen in the indigenous proteins3. (euromedh2020.com)
- Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen in the second lane (+), but not by the corresponding non-phosphopeptide (not shown). (aveslabs.com)
Synthesized phosphopeptide derived1
- The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human Syk that contains tyrosine 323 (tyrosine 317 in the mouse sequence). (thermofisher.cn)
Protein5
- This antibody detects endogenous protein at a molecular weight of 90 kDa. (thermofisher.com)
- Specific for IFNAR1 protein phosphorylated at Ser535,539. (phosphosolutions.com)
- Specific for endogenous levels of the ~135 kDa KCC2 protein phosphorylated at Ser940. (aveslabs.com)
- Immunoprecipitation of phospho-androgen receptor protein from 22Rv1 cell extracts. (cellsignal.jp)
- The modification of STIL at a specific location by PLK4 was needed to link it to another protein in the outer wall of the centriole, and was also necessary for the cells to build new centrioles. (elifesciences.org)
Proteins6
- The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level. (arigobio.cn)
- Phospho-specific antibodies are widely used to identify the activation of signal transduction pathways resulting from the phosphorylation of a diverse set of proteins that include enzymes, receptors, and transcription factors. (neobiolab.com)
- Moreover, these ligands usually do not bind to various other phospho-proteins promiscuously. (euromedh2020.com)
- As a result, antibodies with the capacity of spotting the phosphorylated type of a proteins are precious reagents for biomedical analysis. (euromedh2020.com)
- Due to these limitations, it might be beneficial to develop easy-to-make and inexpensive artificial substances that could become PSSA surrogates, that is certainly, bind and selectively to a specific phospho-form of confirmed proteins tightly. (euromedh2020.com)
- Modulating agents include human E3 ubiquitin ligases, antibodies thereto and variants thereof, as well as related proteins. (justia.com)
Detects endogenous level1
- This antibody detects endogenous level of AFX only when phosphorylated at serine 197. (prosci-inc.com)
Datasheet1
- If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase. (thermofisher.com)
Residues1
- Kinases transfer phosphate groups from ATP to specific serine, threonine or tyrosine residues of target molecules. (wikipedia.org)
Isotype1
- Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is Phospho-Androgen Receptor (Ser310) (E7L2S) Rabbit mAb. (cellsignal.jp)
Calf intestinal phosphatase1
- Western blot analysis of extracts from 293 cells, treatedwith EGF or calf intestinal phosphatase (CIP), using Akt(Phospho-Ser473) Antibody #11054. (sabbiotech.cn)
Monoclonal antibodies2
- Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.com)
- In contrast, hybridoma-based systems for producing monoclonal antibodies are subject to genetic drift and instability, increasing the potential for lot-to-lot variability or loss of antibody expression. (cellsignal.jp)
Phosphatase2
- Upregulation, Antibody-Peptide Competition and Phosphatase Stripping. (thermofisher.cn)
- Western blot analysis of extracts from SW620 cells, untreated or treated with λ phosphatase, using Phospho-β-Catenin (Ser33/37) Antibody. (cellsignal.com)
Secondary Antibody1
- Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody. (cellsignal.jp)
Peptides1
- phosphopeptides are analyzed using mass spectrometry, and peptides are sequenced and analyzed. (wikipedia.org)
Custom antibody1
- Speak to one of our custom antibody specialists to discuss your next custom antibody project. (prosci-inc.com)
Immunization1
- These phosphorylation state-specific antibodies (PSSAs) are nearly always made by immunization of pets with artificial phosphopeptides. (euromedh2020.com)
Rabbits1
- The protocol uses two New Zealand White rabbits that are Specific Pathogen-Free. (neobiolab.com)
Glycerol2
- Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. (prosci-inc.com)
- Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. (ningbobio.com)
Immunoprecipitation1
- However, in order to use this method to investigate SH2 binding sites on a specific target in cell lysate, additional procedures such as pull-down or immunoprecipitation which consume large amounts of sample are required. (biomedcentral.com)
Ser4737
- Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Akt(Phospho-Ser473) Antibody #11054(left) or the same antibody preincubated with blocking peptide(right). (sabbiotech.cn)
- Western blot analysis of extracts from SK-BR-3 cells,treated with insulin and EGF, and pretreated with U0126and LY294002 cells using Akt (Phospho-Ser473) Antibody#11054. (sabbiotech.cn)
- Immunohistochemical analysis of paraffinembeddedhuman Lung carcinoma tissue usingAkt (Phospho-Ser473) Antibody #11054. (sabbiotech.cn)
- Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft, using Phospho-Akt (Ser473) (736E11) Rabbit mAb (left) or PTEN (138G6) Rabbit mAb #9559 (right). (cellsignal.jp)
- Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic and nuclear localization, using Phospho-Akt (Ser473) (736E11) Rabbit mAb. (cellsignal.jp)
- Immunohistochemical analysis using Phospho-Akt (Ser473) (736E11) Rabbit mAb on SignalSlide (R) Phospho-Akt (Ser473) IHC Controls #8101 (paraffin-embedded LNCaP cells, untreated (left) or LY294002-treated (right). (cellsignal.jp)
- Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Phospho-Akt (Ser473) (736E11) Rabbit mAb preincubated with an irrelevant peptide (left) or Phospho-Akt (Ser473) Blocking Peptide (#1140) (right). (cellsignal.jp)
Lysate1
- Further, we showed that PLA-SH2 can survey lung cancer tissues using 1 μl lysate without requiring phospho-enrichment. (biomedcentral.com)
Phosphorylation site of serine1
- AFX (Phospho-Ser197) antibody was raised against a peptide sequence around phosphorylation site of serine 197 (A-A-S (p) -M-D) derived from Human AFX. (prosci-inc.com)
Aliquot2
- It is not recommended to aliquot this antibody. (thermofisher.com)
- Store at -20°C. Do not aliquot the antibody. (cellsignal.com)
Ubiquitin1
- Recombinant TcPINK1 directly phosphorylated ubiquitin and phospho-ubiquitin activated Parkin E3 ubiquitin ligase activity in cell-free assays. (bioseek.eu)
Receptor3
- Flick MB, Sapi E, Perrotta PL, Maher MG, Halaban R, Carter D, Kacinski BM (1997) Recognition of activated CSF-1 receptor in breast carcinomas by a tyrosine 723 phosphospecific antibody. (springer.com)
- using Phospho-Androgen Receptor (Ser310) (E7L2S) Rabbit mAb (upper) or Androgen Receptor (D6F11) XP ® Rabbit mAb #5153 (lower). (cellsignal.jp)
- Western blot analysis was performed using Phospho-Androgen Receptor (Ser310) (E7L2S) Rabbit mAb. (cellsignal.jp)
Recombinant9
- What is a Recombinant Antibody and Why is it Important? (cellsignal.jp)
- Recombinant antibodies offer several key advantages compared to traditional antibodies. (cellsignal.jp)
- As such, recombinant antibodies are seeing increased use for scientific research, especially as a means of addressing the ongoing reproducibility crisis. (cellsignal.jp)
- What is a Recombinant Antibody? (cellsignal.jp)
- Recombinant antibodies are monoclonal, but their production involves in vitro genetic manipulation. (cellsignal.jp)
- Mammalian cell lines are most commonly used for recombinant antibody production, although cell lines of bacterial, yeast, or insect origin are also suitable. (cellsignal.jp)
- Because recombinant antibody production involves sequencing the antibody light and heavy chains, it is a highly controlled and reliable process. (cellsignal.jp)
- Recombinant antibodies are highly consistent from lot to lot, thereby ensuring reproducible experimental results. (cellsignal.jp)
- Unlike traditional methods for antibody production, recombinant approaches avoid the need to use animals. (cellsignal.jp)
Human2
- Phosphospecific peptide around Ser9 (R-L-S(p)-D-S) of Human Synapsin 1. (arigobio.cn)
- Analysis of human tumors indicates that phospho-S48-NPM may be a useful biomarker for monitoring AKT activity and in vivo efficacy of AKT inhibitor treatment. (oncotarget.com)
Peptide synthesis1
- Brandt M, Gammeltoft S, Jensen KJ (2006) Microwave heating for solid-phase peptide synthesis: general evaluation and application to 15-mer phosphopeptides. (springer.com)
Substrate1
- Kinase activity of tissue lysates was measured using PamStation®12 high-content phospho-peptide substrate microarray system. (oncotarget.com)
Primary3
- Like other Alexa Fluor dyes, the Anti-MSK1 Ser376 exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. (ningbobio.com)
- For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween ® 20 at 4°C with gentle shaking, overnight. (cellsignal.com)
- Please refer to primary antibody product webpage for recommended antibody dilution. (cellsignal.com)
Blot3
- Dot blot analysis of anti-Phospho-MEF2C-pS387 Antibody (Cat. (avivasysbio.com)
- Western blot: Extracts from Mouse brain tissue stained with ARG51751 anti-Synapsin 1 phospho (Ser9) antibody. (arigobio.cn)
- Specific immunolabeling of the ~110 kDa to ~130 kDa IFNAR1 WT (2) is shown in the first blot, as the immunolabeling is absent in IFNAR1 Ser535 and Ser539 mutants (3). (phosphosolutions.com)
Membrane1
- OAAB16210) and anti-MEF2C Non Phospho-specific Pab on nitrocellulose membrane. (avivasysbio.com)
Extracts1
- WB analysis of extracts from K562 cells treated with H2O2 using GTX55402 CaMKIV (phospho Thr200) antibody. (genetex.com)
Genes2
- After cloning the antibody genes into an expression vector, this is then transfected into an appropriate host cell line for antibody expression. (cellsignal.jp)
- NF-κB is a transcription factor that plays a pivotal role in the highly specific pattern of gene expression observed for immune, inflammatory and acute phase response genes, including interleukin 1, interleukin 8, tumor necrosis factor and certain cell adhesion molecules. (justia.com)
Vial1
- 1 mg/ml (Please refer to the vial label for the specific concentration. (genetex.com)
Assay2
- The SH2-PLA assay utilizes oligonucleotide-conjugated anti-GST and anti-EGFR antibodies recognizing a GST-SH2 probe and cellular EGFR, respectively. (biomedcentral.com)
- In an oligonucleotide-tagged multiplex (OTM) assay, a mixture of SH2 domains with domain-specific DNA tags are incubated with a sample spot allowing for competitive binding (single sample per well) [ 8 ]. (biomedcentral.com)
Transcription1
- C5DELq14.3;DEL5q14.3;MADS box transcription enhancer factor 2, polypeptide C;Myocyte enhancer factor 2C;myocyte-specific enhancer factor 2C;NEDHSIL. (avivasysbio.com)