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  • phosphoserine
  • All of these products may be used to enrich cytosolic and membrane-bound phosphoproteins or phosphopeptides containing phosphotyrosine, phosphoserine, or phosphothreonine from mammalian cells and tissues. (clontech.com)
  • Using a library of partially degenerate phosphopeptides, we identified tandem BRCT (BRCA1 carboxyl-terminal) domains in PTIP (Pax transactivation domain-interacting protein) and in BRCA1 as phosphoserine- or phosphothreonine-specific binding modules that recognize substrates phosphorylated by the kinases ATM (ataxia telangiectasia-mutated) and ATR(ataxia telangiectasia- and RAD3-related) in response to γ-irradiation. (sciencemag.org)
  • singly
  • The MS-Pillar Phosphopeptide challenge samples are a complex set of human phosphopeptides (Ser, Thr or Tyr) singly and multiply phosphorylated) and their unphosphorylated counterparts that can be used for method development and verification for phosphopeptide enrichment, sequence analysis by mass spectrometry and bioinformatic evaluation. (hupo.org)
  • The MS Resource Pillar has partnered with SynPeptide Co. Ltd in Shanghai ( www.synpeptide.com ) to develop a complex set of human phosphopeptides (Ser, Thr or Tyr) singly and multiply phosphorylated) and their unphosphorylated counterparts that can be used for method development and verification for phosphopeptide enrichment, sequence analysis by mass spectrometry and bioinformatic evaluation. (hupo2017.ie)
  • characterization
  • However, the ability of these techniques to obtain sufficient structural information for unambiguous phosphopeptide identification and characterization is highly dependent on the ion activation method employed and the properties of the precursor ion that is subjected to dissociation. (springer.com)
  • substrate
  • In order to elucidate the mechanism of substrate recognition by CaMKPase, we chemically synthesized a variety of phosphopeptide analogs and carried out kinetic analysis using them as CaMKPase substrates. (semanticscholar.org)
  • beads
  • The phosphopeptide enrichment capabilities of six different resins, Glygen TiO 2 , ZrO 2 , and mixed ZrO 2 and TiO 2 NuTips, Titansphere TiO 2 resin, PhosTrap magnetic titanium beads, and a Fe-NTA resin, are compared. (springer.com)
  • The insolution binding of phosphopeptides to PolyMAC and the covalent capture onto solid-phase beads all occur in a single step. (expedeon.com)
  • Detect
  • To address this analytical challenge, we developed two algorithms to detect separated and co-eluting phosphopeptide isomers and target their subsequent identification using an inclusion list in LC-MS/MS experiments. (jpt.com)
  • bind
  • These findings provide a molecular basis for BRCT domain function in the DNA damage response and may help to explain why the BRCA1 BRCT domain mutation Met 1775 → Arg, which fails to bind phosphopeptides, predisposes women to breast and ovarian cancer. (sciencemag.org)
  • Algorithms
  • Using these algorithms, we determined that the proportion of isomers present in phosphoproteomics studies from mouse, rat, and fly cell extracts represents 3-6% of all identified phosphopeptides. (jpt.com)
  • interaction
  • It was also demonstrated that SAX have the ability to fractionate phosphopeptides under gradient elution based on their different interaction with SAX adsorbent. (uniprot.org)
  • different
  • This difficulty is exacerbated by the combinatorial distribution of phosphorylated residues giving rise to different phosphopeptide isomers. (jpt.com)