• The chemically anchored phospholipid monolayer was prepared by using in situ photopolymerization of a pre-assembled phospholipid monolayer, produced by lipid vesicle fusion, onto the acrylated polymer coated silicon wafer. (ewha.ac.kr)
  • MlaA represents a lipid transport protein that selectively removes outer leaflet phospholipids to help maintain the essential barrier function of the bacterial OM. (nature.com)
  • The structures show that both substrate translocation and release involve movements of the bulky transport domain through the lipid bilayer. (elifesciences.org)
  • The arachidonic acid, released by phospholipase A2, is an important substrate for the production of a group of lipid mediators known as leukotrienes, which induce proinflammatory signaling through the activation of specific BLT and CysLT receptors. (hindawi.com)
  • Despite this setback, SFE is alive and well and being applied to many substrates that contain lipid matter. (aocs.org)
  • Figure 1 illustrates the spectrum of critical fluid-based solvents that can be applied to lipophilic substrates to produce food and nutraceutical ingredients from lipid-containing feedstocks. (aocs.org)
  • These include the generation of a variety of lipid mediators, along with membrane remodelling, modification of extracellular non-cellular phospholipid components of pulmonary surfactant, microparticles and lipoproteins, and degradation of microbial membranes and dietary phospholipids. (ijbs.com)
  • Herein, we addressed the question of how Hsp70 interacts with negatively charged phospholipids in artificial lipid compositions employing the X-ray reflectivity (XRR) studies. (bvsalud.org)
  • Exposure to toxic copper level alters erythrocyte membrane fluidity and deformability by disrupting membrane lipid composition, saturation, bond configuration in phospholipids and permeability. (bvsalud.org)
  • Nanostructured lipid-based films for substrate mediated applications in biotechnology. (lu.se)
  • This asymmetry is maintained by the Mla pathway, a six-component system that is widespread in Gram-negative bacteria and is thought to mediate retrograde transport of misplaced phospholipids from the outer leaflet of the OM to the cytoplasmic membrane 4 . (nature.com)
  • MlaC probably accepts a phospholipid from MlaA and shuttles it to the ABC transporter for ATP-dependent plasma membrane insertion 5 . (nature.com)
  • Long chain polyunsaturated fatty acids (LCPUFA) are bioactive components of membrane phospholipids and serve as substrates for signaling molecules. (biorxiv.org)
  • Alpha GPC supports neuronal health and promotes signal transmission by serving as a precursor to membrane phospholipids. (smallflower.com)
  • The AA, a normal component of cell membrane phospholipids, serve as substrate for prostaglandin endoperoxide (PGH) syntases-1 and -2, also known as cycloxygenase (COX)-1 and -2, lipoxygenase (5-, 12-, or 15) (LO), or cytochrome p450 enzymes [ 18 ]. (hindawi.com)
  • Incorporation of such nanoparticles in Langmuir and Langmuir-Blodgett (LB) films and vesicles of phospholipids, applied as cell membrane models. (sbpmat.org.br)
  • The C2 domain is thought to be involved in calcium-dependent phospholipid binding [ ( PUBMED:8253763 ) ] and in membrane targetting processes such as subcellular localisation. (embl.de)
  • HSPA8 binds with the phospholipids on the late endosomal membrane. (reactome.org)
  • Further studies examine how changes in cell membrane structure mediated by inositol phospholipids lead to production of signals that remodel the cytoskeleton. (upenn.edu)
  • Plasma obtained and RBC membrane prepared from blood collected over EDTA post-treatment were assayed for total cholesterol (TC), phospholipids and fatty acid profile using spectrophotometry and Gas chromatography while heparinized blood was subjected to fragility test. (bvsalud.org)
  • Biophysical study of resin acid effects on phospholipid membrane structure and properties. (lu.se)
  • A similar selective phospholipid peroxidation profile was detected upon incubation of a mixture of total lung lipids with H(2)O(2)/cytochrome c known to catalyze cardiolipin and phosphatidylserine peroxidation in apoptotic cells. (cdc.gov)
  • and the use of food-compatible co-solvents with SC-CO 2 , such as ethanol, extends the range of SFE for processing substrates that can contain polar lipids (such as phospholipids) and related compounds [3]. (aocs.org)
  • The purified enzyme is capable of utilising Ptdlns, PtdIns(4)P and PtdIns(4,5)P2 as substrates, the ratios of phosphorylation of these three lipids were similar with substrates presented as pure sonicated phospholipids. (ucl.ac.uk)
  • In addition to bulk protein degradation, Microautophagy (MI) can also target KFERQ motif protein substrates similar to Chaperone Mediated Autophagy (CMA). (reactome.org)
  • For instance, GIII, GV and GX sPLA 2 s efficiently hydrolyse phosphatidylcholine, while GIIA sPLA 2 has higher activity against negatively charged phospholipid substrates, and in particular, phosphatidylserine, phosphatidylglycerol and phosphatidylethanolamine. (ijbs.com)
  • Peroxidation products were identified in three relatively minor classes of anionic phospholipids, cardiolipin, phosphatidylserine, and phosphatidylinositol, whereby oxygenation of polyunsaturated fatty acid residues also showed unusual substrate specificity. (cdc.gov)
  • The human mutant had 40% of the activity of the wild-type enzyme when assayed using anionic phospholipids, however, a dramatic feature of this mutant was its enhanced activity (250 fold) when assayed with vesicles of phosphatidyl choline. (soton.ac.uk)
  • Activated PI3K phosphorylates the 3′-position of the ring in inositol phospholipids, generating phosphatidylinositol ( 3 , 4 ), diphosphate, and phosphatidylinositol (3,4,5)-triphosphate (PIP3). (diabetesjournals.org)
  • Here we show, using X-ray crystallography, molecular dynamics simulations and in vitro and in vivo functional assays, that MlaA is a monomeric α-helical OM protein that functions as a phospholipid translocation channel, forming a ~20-Å-thick doughnut embedded in the inner leaflet of the OM with a central, amphipathic pore. (nature.com)
  • Binding of insulin to its receptor activates the insulin receptor tyrosine kinase, resulting in autophosphorylation and phosphorylation of several substrates, including insulin receptor substrate (IRS)-1 through -4. (diabetesjournals.org)
  • Annexin I (also called lipocortin 1) is a substrate for the EGF-stimulated tyrosine kinase and is postulated to be involved in mitogenic signal transduction. (rupress.org)
  • Certain very abundant ribosomal proteins can be the sacrificial substrates targeted for degradation at the onset of the downshift. (go.jp)
  • It is involved in the phospholipid metabolic pathway. (hmdb.ca)
  • Choline kinase alpha (ChoKalpha) is an enzyme involved in the metabolism of phospholipids recently found to play a relevant role in the regulation of cell proliferation, oncogenic transformation and human carcinogenesis. (nih.gov)
  • Enzyme interaction with phospholipids was followed by changes in Pi-A curves, and their adsorption on DMPA/Zn2+ or DMPA/trehalose LB films using the quartz crystal microbalance technique, QCM. (usp.br)
  • The purified enzyme was characterised with respect to substrates and potential activators and has a Km for ATP of 67 μM, for pure sonicated Ptdlns of 34 μM and an apparent Ka for Mg2+ of 6.9 mM. (ucl.ac.uk)
  • sPLA 2 s show distinct substrate specificities in terms of the phospholipid polar headgroups and the fatty acids esterified at the sn- 2 position of the glycerol backbone. (ijbs.com)
  • As the transport domain translocates into the IFS, HP2 replaces HP1 on the domains interface, while HP1 now lines an intracellular vestibule leading to the substrate-binding site ( Figure 1-figure supplement 1 ). (elifesciences.org)
  • Particular C2s appear to bind phospholipids, inositol polyphosphates, and intracellular proteins. (embl.de)
  • Upon binding with Hspa8, substrates are transported from the cytosol to late endosomes. (reactome.org)
  • In this Dissertation we have studied the adsorption of two forms of AP in phospholipid Langmuir monolayers and Langmuir-Blodgett films, considered as biomembrane models, at different temperatures. (usp.br)
  • This architecture prevents access of inner leaflet phospholipids to the pore, but allows outer leaflet phospholipids to bind to a pronounced ridge surrounding the channel, followed by diffusion towards the periplasmic space. (nature.com)
  • We used porous substrates with different pore radii serving as a static meshwork to modulate the size of. (lu.se)
  • The deleterious effects of Mla knockouts probably result from phospholipid accumulation in the OM outer leaflet, forming bilayer patches that are portals for entry of lipophilic small molecules 17 . (nature.com)
  • The phospholipid compositions of the invention are well tolerated by human tissue making them suitable for use as preservative and disinfectant components in the preparation of personal care, household cleaning and like products which exhibit enhanced antimicrobial and antifungal characteristics. (justia.com)
  • DG(i-18:0/21:0/0:0) is also a substrate of diacylglycerol kinase. (hmdb.ca)
  • No oxidized molecular species were found in the two most abundant phospholipid classes: phosphatidylcholine and phosphatidylethanolamine. (cdc.gov)
  • The model was used to estimate kinetic parameters of in vitro transport data from organic anion-transporting peptide model substrates (e.g., cholecystokinin octapeptide deltorphin II, fexofenadine, and pitavastatin). (aspetjournals.org)
  • Subsequently, the substrate is transported into the lumen via endosomal sorting complexes required for transport (ESCRTI and ESCRTIII systems) (Sahu R et al. (reactome.org)
  • 2011). This event is represented as a black box since the precise molecular mechanism of the substrate transport into the endosomal lumen is unclear. (reactome.org)
  • Endonexin II is a protein with similar Ca2+ and phospholipid binding properties as lipocortin 1, but it is not a substrate for tyrosine kinases. (rupress.org)
  • Moreover, the polymerized phospholipid monolayer also suppressed albumin absorption and platelet adhesion, in vitro. (ewha.ac.kr)
  • Our mass spectrometry-based oxidative lipidomics analysis of all major phospholipid classes revealed highly selective patterns of pulmonary peroxidation after inhalation exposure of mice to single-walled carbon nanotube s. (cdc.gov)
  • The molecular basis to understand the connection between phospholipids metabolism and cell cycle regulation through choline kinase is reported. (nih.gov)
  • This NIOSH study was conducted to establish the effects of a diesel particulate filter (DPF) system with sintered metal substrate on the concentrations and size distributions of nano and ultrafine aerosols and concentrations of nitric oxides in undergro. (cdc.gov)
  • As a substrate involved in the synthesis and secretion of acetylcholine, Alpha GPC supports brain function and learning processes. (smallflower.com)
  • SLC4 family members differ in their substrate dependence, charge transport stoichiometry, and tissue expression. (tcdb.org)
  • Endothelial cells, fibroblasts, neurons and astrocytes each show unique dependence on both elastic and viscous properties of the substrate, and we seek to understand how they sense and respond to this mechanical cue. (upenn.edu)
  • Only the Mla system maintains asymmetry directly via phospholipid extraction, while PldA and PagP both generate lysophospholipids in the outer leaflet that still require removal. (nature.com)
  • We reviewed a few plausibly organic syntheses of phospholipids under prebiotic conditions with special attention paid to the starting materials as pro-chiral dihydroxyacetone and dihydroxyacetone phosphate (DHAP), which are the key molecules to break symmetry in phospholipids. (mdpi.com)
  • We report Cryo-EM structures of Glt Ph reconstituted into nanodiscs, including those structurally constrained in the cytoplasm-facing state and either apo, bound to sodium ions only, substrate, or blockers. (elifesciences.org)
  • This precompiled information serves as a substrate and matrix to embed your contributions, but it is by no means the final word - Homo sapiens can do much better! (wikigenes.org)
  • The physicochemical properties of polymerized phospholipid monolayer on the substrate were evaluated using water contact angle, field-emission scanning electron micrograph (FE-SEM), atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS). (ewha.ac.kr)
  • MlaA is the most enigmatic Mla component, particularly since its identity as a periplasmically exposed lipoprotein appears hard to reconcile with its proposed activity on outer leaflet phospholipids. (nature.com)
  • A 10-fold enhancement of activity was also seen using whole cells as substrates. (soton.ac.uk)
  • Local attraction of substrates and co-substrates enhances weak acid and base transmembrane transport, and principles in the attraction and facilitation of weak acid and base transport substrates, including monocarboxylates, ammonium, bicarbonate, and arsenite, plus protons as a co-substrate have been elucidated ( Epalle and Beitz 2022 ). (tcdb.org)
  • While these known phospholipids have been found useful as surfactants in a variety of personal care, household cleaning and the like products, such products also require the incorporation of antimicrobial preservatives to inhibit microbial spoilage and increase the shelf life. (justia.com)
  • Lecithin is the most common phospholipid in human bodies. (syromonoed.com)
  • These synthetic phospholipids are suggested as exhibiting an outstanding combination of surfactant characteristics as well as being well tolerated by human tissue, i.e., they exhibit exceptionally low ocular irritation and oral toxicity. (justia.com)
  • This polymerized phospholipid monolayer provides a new biomimetic system for coating medical devises. (ewha.ac.kr)
  • Columnar fractionation-based methods, based on longitudinal temperature gradients along the column, can be operated in either concurrent or countercurrent modes with respect to substrate feeds vs. flow of the critical fluid. (aocs.org)
  • In applying SFE to foods and agricultural materials processing, issues such as the degree of comminution and substrate water content have a significant effect on the resultant extract and must be controlled to ensure the desired result. (aocs.org)
  • These results confirmed that the polymerized phospholipid monolayer was chemically anchored on the acrylated polymer substrate. (ewha.ac.kr)