Ligand binding of a number of growth factors and cytokines can result in the release of mediators known as inositol phosphoglycans (IPG) catalyzed by a glycosyl phosphatidylinositol specific phospholipase D. Two distinct subtractions of IPG derived from M. vaccae were able to mimic metabolic effects of mammalian IPG such as activating pyruvate dehydrogenase phosphatase and stimulating lipogenesis in adipocytes. (ucl.ac.uk)
In addition, phosphatidylinositol-specific phospholipase C treatment released the MT4-MMP from the surface of transfected cells. (ox.ac.uk)
The yeast and plant C-terminal signals were found to be capable of directing the addition of a GPI-anchor to the endoglucanase protein (EGE') as shown by the sensitivity of the lipid component of GPI to phosphatidylinositol-specific phospholipase C (PI-PLC) digestion. (edu.au)
GPLD13
A Rabbit polyclonal antibody against Human Glycosylphosphatidylinositol Specific Phospholipase D1 (GPLD1). (phosphatidylinositol.com)
This led them to identify an enzyme called glycosylphosphatidylinositol specific phospholipase D1 (Gpld1) which is created in the liver. (lifespan.io)
The research described in this proposal will investigate the beneficial effect of the exercise-induced, liver-derived blood factor Glycosylphosphatidylinositol-Specific Phospholipase D1 (GPLD1) on the brain vasculature as a critical mediator in combating drivers of Alzheimer's disease. (curealz.org)
Membrane7
The human alkaline phosphatases (hALP) are found anchored on the cell membrane by glycosylphosphatidylinositol. (statpearls.com)
Arabinogalactan proteins (AGPs) are plant extracellular proteoglycans associated with the plasma membrane by a glycosylphosphatidylinositol (GPI) anchor. (bvsalud.org)
Membrane type 4 matrix metalloproteinase (MT4-MMP, MMP-17) is a glycosylphosphatidylinositol-anchored proteinase. (ox.ac.uk)
Secreted phospholipases A 2 (sPLA 2 s) participate in a very broad spectrum of biological processes through their enzymatic activity and as ligands for membrane and soluble receptors. (ijbs.com)
We have identified CD48, a glycosylphosphatidylinositol-anchored molecule, to be the complementary FimH-binding moiety in rodent mast cell membrane fractions. (ox.ac.uk)
Decay accelerating element (DAF) is definitely a glycosylphosphatidylinositol (GPI)-anchored membrane protein. (biotechpatents.org)
The human cellular prion protein (PrP C ) is a glycosylphosphatidylinositol (GPI) anchored membrane glycoprotein with two N-glycosylation sites at residues 181 and 197. (mdpi.com)
Phosphatidylcholine1
He enjoys an international reputation for his research on phospholipases A, cardiolipin biosynthesis in eukaryotes, lysophospholipases, phosphatidylcholine biosynthesis for lung surfactant, plasmalogen biosynthesis in peroxisomes, diagnosis of peroxisomal disorders and most recently his work on alkyl-dihydroxyacetone phosphate synthase. (ornine.best)
Inositol1
This enzyme catalyses the following chemical reaction 6-(α-D-glucosaminyl)-1-phosphatidyl-1D-myoinositol + H2O ⇌ {\displaystyle \rightleftharpoons } 6-(α-D-glucosaminyl)-1D-myo-inositol + 3-sn-phosphatidate This enzyme cleaves proteins from the lipid part of the glycosylphosphatidylinositol (GPI) anchors. (wikipedia.org)
Tyrosine1
the Src family of tyrosine kinases, phospholipase C (PLC), and is mediated specifically from the IP3R isoform 3. (biotechpatents.org)
Moiety1
This moiety is thought to be cleaved by phospholipase for secretion. (bvsalud.org)
Proteins1
Anchoring of Glycosylphosphatidylinositol- Proteins to Lipsomes -- Section II: Liposomes in Molecular Cell Biology -- Chapter 13. (edu.au)
Cysteine1
Pays, E. / A role for the dynamic acylation of a cluster of cysteine residues in regulating the activity of the glycosylphosphatidylinositol- specific phospholipase C of Trypanosoma brucei . (itg.be)
Specific2
The 7-fold repeat domain is homologous to a similar domain in phosphatidylinositol-glycan-specific phospholipase D. (embl.de)
They are released in the serum by the action of specific phospholipase. (statpearls.com)
Molecular1
Secreted phospholipases A 2 (sPLA 2 s) (EC 3.1.1.4) are a structurally related group of low-molecular-mass enzymes (14-18 kDa) that catalyse the hydrolysis of glycerophospholipids (phospholipids hereafter) at their sn -2 position, to produce lysophospholipids and free fatty acids. (ijbs.com)
Receptor1
Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. (lookformedical.com)
Anchor1
Release of the variant surface antigens of African trypanosomes, agents of a reemerging infectious disease in sub-Saharan Africa, occurs not only by proteolysis but also by glycosylphosphatidylinositol-anchor hydrolysis through a phospholipase present in the parasite surface, was demonstrated. (cdc.gov)
Activity1
Liposomes in the Study of Phospholipase A2 Activity -- Chapter 3. (edu.au)
Cell-surface2
In complementary studies, Alun's group has demonstrated, for the first time, that activation of phospholipase A2 by abnomal forms of PrP at the cell surface results in it binding to type III tubulin, indicating that alterations in neuronal transport systems may contribute to the degenerative effects of these peptides. (cam.ac.uk)
Glypican-1 (GPC1) is a glycosylphosphatidylinositol-anchored cell surface protein. (nature.com)
Factor1
The mast cell tumor necrosis factor alpha response to FimH-expressing Escherichia coli is mediated by the glycosylphosphatidylinositol-anchored molecule CD48. (ox.ac.uk)
Signal1
Such sequences often act as a glycosylphosphatidylinositol (GPI) anchoring signal rather than as a transmembrane domain. (ox.ac.uk)
In vitro, the GPI-anchored proteins cleaved by the GPIase activity of ACE had the same size as those released by the bacterial phosphatidylinositol phospholipase C (PI-PLC), a well-characterized and commonly used GPIase. (medscape.com)
Hydrolysis3
Glycosyl phosphatidylinositol molecules from fetal and adult cells were sensitive to hydrolysis by a phosphatidylinositol-specific phospholipase C from B. cereus. (diabetesjournals.org)
Release of the variant surface antigens of African trypanosomes, agents of a reemerging infectious disease in sub-Saharan Africa, occurs not only by proteolysis but also by glycosylphosphatidylinositol-anchor hydrolysis through a phospholipase present in the parasite surface, was demonstrated. (cdc.gov)
The average of specific activity for pNPP hydrolysis (at pH 10.5) by alkaline phosphatase released by phosphatidylinositol-specific phospholipase C (PIPLC) from Bacillus cereus among different bone regions at different animal ages was 1,142.57 U.mg-1, while for PPi hydrolysis (at pH 8.0), it was 1,433.82 U.mg-1. (bvsalud.org)
MRNA1
7. [Expression and activity of glycosylphosphatidylinositol-specific phospholipase d mRNA in bone marrow mononuclear cells isolated from patient with acute myeloid leukemia and their significance]. (nih.gov)
Metabolism1
Furthermore, all the differential metabolite were mapped to KEGG pathway, revealing significant differences in linoleic acid metabolism, tyrosine metabolism，glutathione metabolism, pentose phosphate pathway, tropane, piperidine and pyridine alkaloid biosynthesis, phenylpropanol metabolism and glycosylphosphatidylinositol(GPI)-anchor biosynthesis over time. (researchsquare.com)
Assay3
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycosylphosphatidylinositol Specific Phospholipase D1 were tested 20 times on one plate, respectively. (phosphatidylinositol.com)
15. [Methodological study on the assay of glycosylphosphatidylinositol-specific phospholipase D activity in serum]. (nih.gov)
Treatment of whole cell TX-100 lysates or sucrose gradient fractions dotted onto nitrocellulose filter strips with bacterial phosphatidylinositol-specific phospholipase C (PI-PLC) proved to be an efficient method to assay for the presence of a GPI-anchor in Thy-1 and Ly6 surface glycoproteins. (unige.ch)
Proteolytic1
Proteolytic cleavage of PrP C . After cleavage of the N-terminal and GPI anchor signal peptides (grey), mature membrane-bound PrP C can undergo at least three different proteolytic cleavage events (scissors) in addition to phospholipase C cutting within its GPI anchor. (biomedcentral.com)
Primarily2
NgR2, also known as reticulon 4 receptor-like 2 and NgR2, is a 60 kDa glycosylphosphatidylinositol-anchored protein that is expressed primarily in the brain. (rndsystems.com)
This gene encodes a glycosylphosphatidylinositol (GPI)-specific phospholipase that primarily localizes to the Golgi apparatus. (nih.gov)
Gene1
2. Effect of glycosylphosphatidylinositol specific phospholipase D gene expression levels on complement mediated killing of leukemic cells in patients with chronic myeloid leukemia. (nih.gov)
Activity1
Required for phospholipase A2 activity that removes an acyl-chain at the sn-2 position of GPI-anchors during the remodeling of GPI (Probable). (nih.gov)
Treatment1
TSP-1-mediated tumor cell invasion was almost completely inhibited by either antisense uPAR inhibition or treatment with phospholipase C or ε- aminocaproic acid. (elsevierpure.com)
Form2
Whereas ceruloplasmin in plasma is produced and secreted by hepatocytes, in the brain a glycosylphosphatidylinositol (GPI)-anchored form of ceruloplasmin is expressed on the surface of astrocytes. (nih.gov)
A novel glycosylphosphatidylinositol-anchored form of ceruloplasmin is expressed by mammalian astrocytes. (nih.gov)
Cell1
Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. (lookformedical.com)