Phosphocreatine kinase. Medical search. Frequent questions

The experimental models were given standard cooling therapy (therapeutic hypothermia) alone and in combination with a selective Src kinase inhibitor, PP2, that blocks a regulatory enzyme of apoptosis (cell death), which intensifies as a result of hypoxia-ischemia. (news-medical.net)
In oxygen deprivation of the brain, the pathways leading to cell death are over-activated, including the nuclear enzyme CaM kinase IV. (news-medical.net)
Magnesium plays an important role in regulating muscle function as a cofactor for creatine kinase, an enzyme that generates ATP from phosphocreatine stored in muscle tissues during intense exercise. (purepharmacy.com)
This test measures the blood level of CK-MB (creatine kinase myocardial band), the bound combination of two variants (isoenzymes CKM and CKB) of the enzyme phosphocreatine kinase. (lotusdiagnostic.com)
Mitochondrial creatine kinase, MtCK, fixed by cardiolipin molecules in the vicinity of the adenine nucleotide translocator, is a key enzyme in this pathway. (hal.science)
Creatine kinase, also known as phosphocreatine kinase or creatine phosphokinase, is an enzyme or type of protein that is found in several tissue types of the human body, including the muscle and the brain. (creatinejournal.com)
The function of this enzyme is to catalyze the conversion of creatine to phosphocreatine by applying itself in the consumption of adenosine triphosphate, the generation of adenosine diphosphate, and the reverse reaction. (creatinejournal.com)
Creatine kinase is an enzyme which catalyses the interconversion of creatine and phosphocreatine (PCr). (unboundmedicine.com)
Creatine interacts with an enzyme process called creatine kinase (CK) where it picks up a phosphate molecule and becomes creatine phosphate also called phosphocreatine (CP). (tigerfitness.com)
Creatine kinase (CK) is an enzyme whose function is to catalyze the combination of creatine and adenosine triphosphate (ATP) to form phosphocreatine and adenosine diphosphate (ADP), an essential step for cellular energy generation and metabolism. (pediatriconcall.com)
In our body, creatine can bind a high-energy phosphate group (Pi) forming phosphocreatine (Pcr) (or creatine phosphate (CP)) with help of an enzyme called creatine kinase (CK). (cregaatine.com)
Although the exact mechanisms causing Dox-induced cytotoxicity are not entirely understood, an alteration in the phosphocreatine system seems to be a central mechanism where studies showed Dox decreased phosphocreatine (PCr), creatine (Cr), impaired creatine kinase (CK) enzyme function, and decreased creatine transporter (CrT) expression in cultured cardiomyocytes. (unco.edu)
CREATINE KINASE (CK), also known as creatine phosphokinase (CPK), is an enzyme (EC 2.7.3.2) expressed predominantly in skeletal muscle, smooth muscle and the brain. (gentaur.nl)
BioAssay Systems' EnzyChromTM Creatine Kinase Assay Kit is based on enzyme coupled reactions in which creatine phosphate and ADP is converted to creatine and ATP by CK, the generated ATP is used to phosphorylate glucose by hexokinase to generate glucose-6-phosphate, which is then oxidized by NADP in the presence of glucose-6-phosphate dehydrogenase. (gentaur.nl)

The synthesis of myocardial ATP involves the conversion of phosphocreatine to creatine catalyzed by creatine kinase. (biomedcentral.com)

The creatine kinase phosphotransfer network: thermodynamic and kinetic considerations, the impact of the mitochondrial outer membrane and modelling approaches. (hal.science)
A screen using pooled short hairpin RNAs (shRNAs) identified the ATP-buffering, mitochondrial creatine kinase CKMT1 as necessary for survival of EVI1-expressing cells in subjects with EVI1-positive AML. (northwestern.edu)
CKMT1 inhibition altered mitochondrial respiration and ATP production, an effect that was abrogated by phosphocreatine-mediated reactivation of the arginine-creatine pathway. (northwestern.edu)
Meyer's electrical analog model proposed the concept that the τ is given by τ = R m ·C, where R m is mitochondrial resistance to energy transfer, and C is metabolic capacitance, determined primarily by the cellular total creatine pool (TCr = phosphocreatine + creatine). (elsevierpure.com)

The process of supplementing creatine increases the overall pool of creatine and phosphocreatine in the cells. (hrsupplements.com)

CK catalyzes the conversion of creatine to phosphocreatine, consuming adenosine triphosphate (ATP) and generating adenosine diphosphate (ADP) and the reverse reaction. (gentaur.nl)

The combined results indicate that glycolysis is regulated by the compartmental expression of hexokinase 2, pyruvate kinase M1, and pyruvate kinase M2 in photoreceptors, whereas the inner retinal neurons exhibit a lower capacity for glycolysis and aerobic glycolysis. (molvis.org)
Muscle biopsy specimens from CP patients showed elevated levels of transcript for pyruvate dehydrogenase kinase, phosphofructokinase, and muscle pyruvate kinase. (ox.ac.uk)

The reaction stores ATP energy as phosphocreatine. (ucdenver.edu)
The previously published model of oxidative phosphorylation in isolated skeletal muscle mitochondria was extended by incorporation of the creatine kinase system (creatine kinase plus phosphocreatine/creatine pair), cytosolic proton production/consumption system (proton production/consumption by the creatine kinase-catalysed reaction, efflux/influx of protons), physiological size of the adenine nucleotide pool and some additional minor changes. (physiomeproject.org)
Typically, the forward flux through the creatine kinase reaction is investigated by observing the decrease in phosphocreatine (PCr) after saturation of γ-ATP. (ox.ac.uk)
31P-NMR, with saturation transfer, demonstrates that the phosphocreatine level rises ~2 fold and that the "pseudo" first order rate constant for the creatine kinase reaction falls to ~20% of the control by the onset of nuclear membrane breakdown. (cuny.edu)
The Pi is separated from PCr in the creatine kinase reaction and donated to ADP to reform ATP. (girlsgonestrong.com)

Creatine supplementation increases the levels of phosphocreatine (PCr) in muscles, which is used by creatine kinase to regenerate Adenosine triphosphate ( ATP ) in skeletal muscle . (physio-pedia.com)
in the skeletal muscle, the brain, and the smooth muscle - or all tissues that swiftly use up adenosine triphosphate - phosphocreatine acts as an energy reservoir for the quick regeneration of adenosine triphosphate. (creatinejournal.com)
RB2 activates CK-MM activity in vitro and in vivo, which increases the levels of tissue phosphocreatine and strengthens the function of the creatine kinase/phosphocreatine system in skeletal muscle, thus buffering cellular ATP, delaying exercise-induced lactate accumulation, and improving exercise performance. (strongsupplementshop.com)
During exercise, CP patients had early and marked phosphocreatine depletion and acidosis in skeletal muscle, greater accumulation of lactate in blood, and reduced maximum exercise capacities. (ox.ac.uk)

In this review, we summarize the main structural and functional data on the role of the phosphocreatine (PCr)--creatine kinase (CK) pathway for compartmentalized energy transfer in cardiac cells. (hal.science)
Creatine monohydrate supplementation increases creatine phosphate and creatine kinase in the muscles to produce energy for the phosphocreatine bioenergetic pathway. (xbodyconcepts.com)

Expression of nucleoside diphosphate kinase, mitochondria-associated adenylate kinase, and several mitochondria-associated creatine kinase isozymes was highest in the outer retina, whereas expression of cytosolic adenylate kinase and brain creatine kinase was higher in the cones, horizontal cells, and amacrine cells indicating the diversity of ATP-buffering strategies among retinal neurons. (molvis.org)

Phosphagen (guanidino) kinases mostly found in eukaryotes. (nih.gov)
Phosphagen (guanidino) kinases are enzymes that transphosphorylate a high energy phosphoguanidino compound, like phosphocreatine (PCr) in the case of creatine kinase (CK) or phosphoarginine in the case of arginine kinase, which is used as an energy-storage and -transport metabolite, to ADP, thereby creating ATP. (nih.gov)
Besides CK and AK, the most studied members of this family are also other phosphagen kinases with different substrate specificities, like glycocyamine kinase (GK), lombricine kinase (LK), taurocyamine kinase (TK) and hypotaurocyamine kinase (HTK). (nih.gov)

Mitochondria were incubated in a medium containing 5.0 mM ATP, TCr pools of 0-1.5 mM, excess creatine kinase, and an ATP-splitting system of glucose + hexokinase (HK). (elsevierpure.com)

It is then transported through blood to the other organs, muscle, and brain, where, through phosphorylation, it becomes the high-energy compound phosphocreatine. (wikipedia.org)
In your muscles, creatine combines with a phosphate (Pi) molecule to create a compound called phosphocreatine (PCr). (girlsgonestrong.com)

ARGK-1 and its mammalian equivalent, creatine kinase, are enzymes that transport energy in the form of phosphoarginine or phosphocreatine to various locations within cells. (sbpdiscovery.org)

Interestingly, supplements with creatine, the substrate for creatine kinase, have been found to improve health and longevity in mice and possibly provide beneficial effects to humans with Parkinson's disease, suggesting a potential role for these enzymes in aging and age-related diseases," added McQuary. (sbpdiscovery.org)

We found that longevity can be extended by increasing the amount of a protein called arginine kinase-1 (ARGK-1)," said Philip McQuary, Ph.D., first author of the study and a former graduate student in the laboratory of Malene Hansen, Ph.D. , associate professor in the Development, Aging, and Regeneration Program at SBP. (sbpdiscovery.org)
The research team found that, as in worms, creatine kinase levels are increased in the brains of mice lacking a similar S6K protein. (sbpdiscovery.org)
Perilipin 5 (PLIN5) is a lipid-droplet-associated protein that coordinates intracellular lipolysis in highly oxidative tissues and is thought to regulate lipid metabolism in response to phosphorylation by protein kinase A (PKA). (uci.edu)
The protein, adenosine monophosphate kinase (AMPK), facilitates the conversion of AMP back to ADP. (hrsupplements.com)
The levels of phosphorylated AMP-activated protein kinase (P-AMPK), well known for its role in cellular energy sensing and regulation, and ATP show reciprocal changes. (jneurosci.org)
Furthermore, the changes in ATP exhibit reciprocity with the phosphorylated state of the cellular energy sensor, phosphorylated AMP-activated protein kinase (P-AMPK), thus supporting the induction of anabolic processes during sleep. (jneurosci.org)

By increasing intracellular creatine content, oral creatine ingestion conceivably stimulates operation of the creatine kinase (CK)/phosphocreatine (PCr) system, which in turn facilitates muscle relaxation. (nih.gov)
Also, creatine, creatinine and/or creatine kinase (CK aka phosphocreatine kinase or creatine phosphokinase) levels are often checked for such things as evaluating muscle and renal function, so I would be interested if anyone happens to have lab results for those. (pwsnotes.org)
On the other hand, if the test reveals that the level of creatine kinase circulating in the blood is higher than it should be in normal conditions, then chances are that the human body in question has suffered damage either to the muscle or the brain. (creatinejournal.com)
If most of this sounds like gibberish to you, just remember that a heart attack, a muscle disease or a stroke may result in abnormally raised creatine kinase levels in the blood. (creatinejournal.com)
a source of energy in the contraction of vertebrate muscle , its breakdown furnishing phosphate for the resynthesis of ATP from ADP by creatine kinase . (en-academic.com)
phosphocreatine - (= creatine phosphate) Present in high concentration (about 20mM) in striated muscle, and is synthesized and broken down by creatine phosphokinase to buffer ATP concentration. (en-academic.com)

The CaM kinase IV activity in cerebral tissue was 2,002 (plus or minus 729) with normal oxygen levels and in normal temperatures, 4,104 (plus or minus 542) in hypoxia with hypothermia treatment, and 2,165 (plus or minus 415) in hypoxia with hypothermia treatment combined with PP2 administration. (news-medical.net)
alcohol liver disease and rheumatoid arthritis are two of the most common possibilities that exist with respect to lowered levels of creatine kinase. (creatinejournal.com)
Does creatine supplementation (i.e for body building) have any effect on the creatine kinase levels in the body? (creatinejournal.com)
as ATP levels diminish with muscular exertion, the phosphoryl group of phosphocreatine can be donated to ADP to replenish ATP. (en-academic.com)

Creatine kinase (CK), inherited metabolic disorders (IMD), metabolic myopathies (MM). (pediatriconcall.com)

Creatine is pivotally important in delaying ATP depletion during anoxia or ischemia through the creatine-phosphocreatine system [ 10 ,11]. (oatext.com)

Creatinine itself is produced via a biological system involving creatine, phosphocreatine (also known as creatine phosphate), and adenosine triphosphate (ATP, the body's immediate energy supply). (wikipedia.org)
Cellular ATP is buffered by specialized equilibrium-driven high-energy phosphate (~P) transferring kinases. (molvis.org)
This suggests that MGCs utilize TCA cycle anaplerosis and cataplerosis to generate GTP and ~P transferring kinases to produce ATP that supports MGC energy requirements. (molvis.org)
Your body stores creatine as phosphocreatine primarily in your muscles, where it's used for energy. (physio-pedia.com)
Beyond this point, the phosphocreatine system still provides energy but just to a lesser degree and continues to decrease as exercise continues. (tigerfitness.com)
When phosphocreatine stores are rapidly depleted, glycogenolysis is not able to make up for the decrease in energy production and power output will decrease. (tigerfitness.com)
Phosphocreatine system is the fastest energy system in our body, as it can synthesize the pool of ATP faster than cell respiration. (cregaatine.com)
Increasing the overall pool of creatine, phosphocreatine and nitrate in the cells results in more energy and higher power output during intense training sessions, allowing you to last longer in the gym and stack those gains. (hrsupplements.com)
Creatine associates with creatine kinase to produce ATP for energy production. (oatext.com)

Creatine Kinase" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (ucdenver.edu)

Macro-creatine kinase refers to creatine kinase complexed with other serum proteins. (ucdenver.edu)

however, the effects of Cr supplementation on Dox-induced cytotoxicity specific to the phosphocreatine system have yet to be explored. (unco.edu)

In hypoxia-ischemia, CaM kinase is over-activated, but hypothermia has been shown to decrease this enzyme's activation. (news-medical.net)

Chemically known as α-methyl guanidine-acetic acid , creatine is predominantly stored (about 95%) in your skeletal muscles (as phosphocreatine), which explains why it's one of the top bodybuilding supplements for improving lean mass and exercise performance. (primevallabs.com)
or whether muscles in their bodies have been damaged as a result of any sort of activity, should make it a point to go for a creatine kinase test. (creatinejournal.com)

Creatine kinase will then remove the phosphate molecule from CP and it is donated to ADP to create ATP. (tigerfitness.com)

A transferase that catalyzes formation of PHOSPHOCREATINE from ATP + CREATINE. (ucdenver.edu)

Detection range: 5 to 300 U/L creatine kinase in 96-well plate assay. (gentaur.nl)

Creatine supplementation has the ability to delay fatigue caused by depleted phosphocreatine stores by increasing the storage of creatine phosphate. (tigerfitness.com)
Phosphocreatine stores: These stores, consumed in the purine nucleotide cycle, are vital for very high-intensity exercise of short duration, as other stores are depleted early. (medscape.com)

We theorized that a Src kinase inhibitor, in addition to hypothermia, would further attenuate the activation of CaM kinase IV and that the result might be less brain damage,' explains Panagiotis Kratimenos, M.D., Ph.D., the study's lead author, and a specialist in neonatology and neonatal neurocritical care at Children's National. (news-medical.net)
However, the combination of hypothermia with Src kinase inhibition following hypoxia further attenuated the increased activation of CaM kinase IV, compared with hypothermia alone in the newborn experimental model brain. (news-medical.net)

Taking the creatine test is a good idea to find out where exactly it is that one stands when it comes to the prevalent level of creatine kinase in one's body. (creatinejournal.com)

This is a very important function, and even though it doesn't sound like much, creatine kinase definitely has its work cut out. (creatinejournal.com)

In normal conditions, there is very little creatine kinase circulating in the blood of the average, healthy human being. (creatinejournal.com)

This graph shows the total number of publications written about "Creatine Kinase" by people in this website by year, and whether "Creatine Kinase" was a major or minor topic of these publications. (ucdenver.edu)
Anesthesia Central , anesth.unboundmedicine.com/anesthesia/view/GDT/619114/all/Creatine_kinase__total. (unboundmedicine.com)

Unit definition: one unit of CK will transfer 1 µmole of phosphate from phosphocreatine to ADP per min at pH 6.0. (gentaur.nl)

Anatomy17

Organisms8

Diseases4

Chemicals and Drugs141

Analytical, Diagnostic and Therapeutic Techniques and Equipment11

Phenomena and Processes37

Anthropology, Education, Sociology and Social Phenomena4

Information Science3

Enzyme14

Catalyzed by creatine kinase1

Mitochondrial4

Creatine and phosphocreatine1

Adenosine Diphosphate1

Pyruvate kinase2

Reaction5

Skeletal muscle4

Pathway2

Cytosolic1

Phosphagen3

Hexokinase1

Compound2

Phosphoarginine1

Substrate1

Protein6

Muscle6

Levels4

Metabolic1

Depletion1

Energy9

MeSH1

Serum1

System1

Decrease1

Muscles2

Molecule1

Catalyzes1

Range1

Stores2

Brain2

Test1

Function1

Human1

Total2

Transfer1