• Description: Using a radiometric protein kinase ( 33 PanQinase activity) assay. (guidetopharmacology.org)
  • Here, we show that cyclindependent kinase 5 (Cdk5), a protein kinase that regulates cell migration and invasion, phosphorylates PIPKIγ90 at S453, and that Cdk5-mediated PIPKIγ90 phosphorylation is essential for cell invasion. (arizona.edu)
  • Smooth muscle cells express Kv7.4 and Kv7.5 voltage-dependent potassium channels, which have each been implicated as regulators of smooth muscle contractility, though they display different sensitivities to signaling via cAMP/protein kinase A (PKA) and protein kinase C (PKC). (aspetjournals.org)
  • Protein kinase D regulates metabolism and growth by controlling secretion of insulin like peptide. (ncbs.res.in)
  • We tested the hypothesis that protein kinase C (PKC) participates in stimulus-secretion coupling. (jneurosci.org)
  • The ↑[Ca 2+ ] i activates a signaling cascade that involves, sequentially, calcium-calmodulin, nitric oxide synthase, guanylyl cyclase, and protein kinase G. This signal transduction pathway enables Cl − to pass through vesicular channels and act as a counterion for the transport of H + , so that the vesicles of secretogogue-stimulated cells become acidic. (jneurosci.org)
  • Orthologous to human PIK3C2A (phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha). (nih.gov)
  • QY Research Medical published a comprehensive research on Phosphatidylinositol 4,5 Bisphosphate 3 Kinase Catalytic Subunit Beta Isoform Market, which provides a business strategies, research & development activities, concise outline of the market valuation, valuable insights pertaining to market share, size, supply chain analysis, competitive landscape and regional proliferation of this industry. (prbulletin.com)
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  • This model suggests that GBF1 binding of poliovirus 3A proteins is definitely important vis-à-vis recruitment of phosphatidylinositol 4-kinase class III catalytic subunit β (PI4KIIIβ) to replication of complex membranes. (health-ground.com)
  • PI3K is composed of two subunits: the p85 regulatory subunit, containing two Src homology-2 domains, and the p110 catalytic subunit ( 2 - 4 ). (diabetesjournals.org)
  • Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. (antibodies-online.com)
  • The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. (antibodies-online.com)
  • Type III PI4Ks are structurally linked to phosphatidylinositol 3-kinases (PI3K) because the C-terminal catalytic site and a helical lipid kinase exclusive site (LKU) both are extremely conserved and both lipid kinase family members share a level of sensitivity to wortmannin (16 25 Besides that small is well known about structural determinants of PI4KIIIα playing a job in enzymatic function. (colinsbraincancer.com)
  • The phosphatidyl inositol 3-kinase (PI3K)/Akt pathway was involved in this ubiquitin-proteasome-mediated degradation of PDCD4. (plos.org)
  • Phosphoinositide 3-kinase (PI3K) plays a vital role in plant response to abiotic stress. (frontiersin.org)
  • Phosphoinositide 3-kinase (PI3K) as a key regulator of lipid signal has been reported to be involved in autophagy. (frontiersin.org)
  • Signaling through the phosphatidylinositol 3′-kinase (PI3K) pathway is crucial for metabolic responses to insulin, and defects in PI3K signaling have been demonstrated in type 2 diabetes. (diabetesjournals.org)
  • PTEN (MMAC1) is a lipid/protein phosphatase that can negatively regulate the PI3K pathway by dephosphorylating phosphatidylinositol (3,4,5)-triphosphate, but it is unclear whether PTEN is physiologically relevant to insulin signaling in vivo. (diabetesjournals.org)
  • Phosphatidylinositol 3′-kinase (PI3K) is a crucial signaling enzyme whose activity is regulated by a variety of biological stimuli, including insulin ( 1 ). (diabetesjournals.org)
  • Activated PI3K phosphorylates the 3′-position of the ring in inositol phospholipids, generating phosphatidylinositol ( 3 , 4 ), diphosphate, and phosphatidylinositol (3,4,5)-triphosphate (PIP3). (diabetesjournals.org)
  • We investigated the role of phosphatidylinositol-4-phosphate 5-kinase alpha (PIP5Kα), a key upstream factor of PI3K/AKT, and the therapeutic effect of PIP5Kα inhibitor on subtypes of BC. (lu.se)
  • A previous screen of a human kinase and phosphatase shRNA library to select genes that mediate arsenite induction of spindle abnormalities resulted in the identification of phosphatidylinositol-5-phosphate 4-kinase type-2 gamma (PIP4KIIγ), a phosphatidylinositol 4,5-bisphosphate (PIP2)-synthesizing enzyme. (biomedcentral.com)
  • In addition, PIP2 binds to polo-like kinase (PLK1) and reduces PLK1-mediated phosphorylation of MCAK. (biomedcentral.com)
  • Phosphatidylinositol 4,5-bisphosphate (PI4,5P2, hereafter referred to as PIP2) either binds to intracellular proteins and directly modulates their subcellular localization and activity or acts as a precursor for the generation of other second messengers. (biomedcentral.com)
  • Because the members of the PIP5KI and PIP4KII families exhibit specific and distinct subcellular localization patterns, it has been postulated that local increases in PIP2 concentration by a specific kinase member may underlie its diverse signaling functions [ 5 , 8 ]. (biomedcentral.com)
  • A dPIP5K dependent pool of phosphatidylinositol 4,5 bisphosphate (PIP2) is required for G-protein coupled signal transduction in Drosophila photoreceptors. (ncbs.res.in)
  • The product Assay kit for Guinea pig Phosphatidylinositol 4-Kinase Catalytic Alpha (ELISA) is intended to be used for research purposes only. (phosphatidylinositol.com)
  • The product Assay kit for Guinea pig Phosphatidylinositol 4-Kinase Catalytic Alpha (ELISA) should be kept between two and eight degrees Celsius to ensure the retention of the stability and reactivity of the reagents included in the kit. (phosphatidylinositol.com)
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphatidylinositol-4-Kinase Catalytic Alpha (PI4Ka) were tested 20 times on one plate, respectively. (biomatik.com)
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphatidylinositol-4-Kinase Catalytic Alpha (PI4Ka) were tested on 3 different plates, 8 replicates in each plate. (biomatik.com)
  • This assay has high sensitivity and excellent specificity for detection of Phosphatidylinositol-4-Kinase Catalytic Alpha (PI4Ka). (biomatik.com)
  • A novel mass assay to measure phosphatidylinositol-5-phosphate from cells and tissues. (ncbs.res.in)
  • In enzymology, a 1-phosphatidylinositol 4-kinase (EC 2.7.1.67) is an enzyme that catalyzes the chemical reaction ATP + 1-phosphatidyl-1D-myo-inositol ⇌ {\displaystyle \rightleftharpoons } ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate Thus, the two substrates of this enzyme are ATP and 1-phosphatidyl-1D-myo-inositol, whereas its two products are ADP and 1-phosphatidyl-1D-myo-inositol 4-phosphate. (wikipedia.org)
  • The systematic name of this enzyme class is ATP:1-phosphatidyl-1D-myo-inositol 4-phosphotransferase. (wikipedia.org)
  • This enzyme participates in inositol phosphate metabolism and phosphatidylinositol signaling system. (wikipedia.org)
  • Phosphatidylinosilol 3-kinase (Ptdlns 3-kinase) activity is circumstantially correlated with mitogenic responses and transformation, however little is known about the enzyme. (ucl.ac.uk)
  • The purified enzyme is capable of utilising Ptdlns, PtdIns(4)P and PtdIns(4,5)P2 as substrates, the ratios of phosphorylation of these three lipids were similar with substrates presented as pure sonicated phospholipids. (ucl.ac.uk)
  • The lipid kinase phosphatidylinositol 4-kinase III alpha (PI4KIIIα) is an endoplasmic reticulum (ER)-resident enzyme that synthesizes phosphatidylinositol 4-phosphate (PI4P). (colinsbraincancer.com)
  • The Phosphatidylinositol 4-Kinase Catalytic Alpha ELISA kit is a α- or alpha protein sometimes glycoprotein present in blood.Guinea pig ELISA kits for plasma and sera samples are used to study human genes through the guinea pig model (Cavia porcellus), also called the cavy rodent model. (phosphatidylinositol.com)
  • No significant cross-reactivity or interference between Phosphatidylinositol-4-Kinase Catalytic Alpha (PI4Ka) and analogues was observed. (biomatik.com)
  • moreover, PI3′-kinase inhibitors (wortmannin and LY294002) antagonized secretion. (jneurosci.org)
  • Bithiazole Inhibitors of Phosphatidylinositol 4-Kinase (PI4KIIIβ) as Broad-Spectrum Antivirals Blocking the Replication of SARS-CoV-2, Zika Virus and Human Rhinoviruses. (cdc.gov)
  • Dominant missense mutations in a novel yeast protein related to mammalian phosphatidylinositol 3-kinase and VPS34 abrogate rapamycin cytotoxicity. (yeastgenome.org)
  • Functional analysis of the biochemical activity of mammalian phosphatidylinositol 5 phosphate 4-kinase enzymes. (ncbs.res.in)
  • Recent work has suggested the binding of the protein Golgi-specific brefeldin A resistance guanine nucleotide exchange element 1 (GBF1) by enteroviral 3A is required for the secretion phenotype and viral replication (2 21 Recent work has also demonstrated the importance of the phosphatidylinositol 4-phosphate (PI4P) composition of membranes associated with positive-stranded-RNA replication (17). (health-ground.com)
  • Moreover, inhibition of Ca(2+)-activated secretion by PtdIns(4,5)P2-specific antibodies and phospholipase C implies that 5-phosphorylated inositides play a novel, necessary role in the regulated secretory pathway. (nih.gov)
  • The PI 3-kinase PI3KC2α regulates mouse platelet membrane structure and function independently of membrane lipid composition. (nih.gov)
  • Phosphatidylinositol 4-phosphate 5-kinase type I g (PIPKIγ90) regulates cell migration, invasion, and metastasis. (arizona.edu)
  • Phosphatidylinositol 5 Phosphate 4-Kinase Regulates Plasma-Membrane PIP Turnover and Insulin Signaling. (ncbs.res.in)
  • Phosphatidylinositol 5-phosphate 4-kinase regulates early endosomal dynamics during clathrin-mediated endocytosis. (ncbs.res.in)
  • The results indicate that lipid kinase-mediated phosphorylation is an important basis for ATP use in the exocytotic pathway. (nih.gov)
  • Point mutations in 3A that get rid of PI4KIIIβ association sensitized Aichi computer virus to PIK93 suggesting that disruption of the 3A/ACBD3/PI4KIIIβ complex may represent a novel target for restorative intervention that would be complementary to the inhibition of the kinase activity itself. (health-ground.com)
  • Septins tune lipid kinase activity and PI(4,5)P turnover during G-protein-coupled PLC signalling in vivo. (ncbs.res.in)
  • Common themes to emerge from studies of picornavirus replication are the need for cholesterol and phosphatidylinositol 4-phosphate (PI4P). (microbiologyresearch.org)
  • In infected cells, type III phosphatidylinositol 4-kinases (PI4KIIIs) generate elevated levels of PI4P, which is then exchanged for cholesterol at replication organelles. (microbiologyresearch.org)
  • With this model the switch in phosphoinositol membrane lipid composition resulting from PI4 kinase activity is definitely expected to directly recruit the viral-RNA-dependent RNA polymerase via its PI4P-binding website. (health-ground.com)
  • In brief cells were fixed in 4% paraformaldehyde (PFA) for 20 min and permeabilized with 50 μg/ml digitonin for 5 min for imaging of intracellular PI4P or with 0.5% Triton X-100 for 15 min for colocalization analysis of HA-tagged proteins with HCV NS5A. (colinsbraincancer.com)
  • PF cells were found to express regulatory (p85) and catalytic (p110α and p110β) subunits of phosphatidylinositol 3′-kinase (PI3′-kinase). (jneurosci.org)
  • PKC involvement can be accounted for by PKCγ and an isoform sensitive to inhibition by pseudoZ, probably PKCζ, which is activated via PI3′-kinase. (jneurosci.org)
  • Insulin, after binding its receptor, induces the phosphorylation of receptor substrates in the liver and muscles, and induces several steps toward the transactivation of GLUT-4, which increases glucose uptake by cells and its storage as glycogen, and inhibits the net production of glucose by the liver, thus blocking glycogenolysis and neoglycogenesis. (isciii.es)
  • Design of drug-resistant alleles of type-III phosphatidylinositol 4-kinases using mutagenesis and molecular modeling. (microbiologyresearch.org)
  • Other names in common use include phosphatidylinositol kinase (phosphorylating), phosphatidylinositol 4-kinase, phosphatidylinositol kinase, type II phosphatidylinositol kinase, PI kinase, and PI 4-kinase. (wikipedia.org)
  • PEP3 was recently identified as phosphatidylinositol transfer protein (PtdInsTP), and here we report that PEP1 consists of the type I phosphatidylinositol-4-phosphate 5-kinase (PtdInsP5K). (nih.gov)
  • ROS may induce intracellular calcium increase and subsequent contraction of PASMCs via direct or indirect interactions with protein kinases, phospholipases, sarcoplasmic calcium channels, transient receptor potential channels, voltage-dependent potassium channels and L-type calcium channels, whose relevance may vary under different experimental conditions. (ersjournals.com)
  • Four such genes, HPS1, ADTB3A, HPS3, and HPS4, are associated with the 4 known subtypes of Hermansky-Pudlak syndrome: Hermansky-Pudlak syndrome type 1 (HPS-1), Hermansky-Pudlak syndrome type 2 (HPS-2), Hermansky-Pudlak syndrome type 3 (HPS-3), and Hermansky-Pudlak syndrome type 4 (HPS-4). (medscape.com)
  • Our findings showed that Ang II stimulated rapid and significant activation of extracellular signal-regulated kinase (ERK) 1/2, c-Jun N-terminal kinase (JNK), and p38 in RASMC. (aspetjournals.org)
  • TOR1 and TOR2 are structurally and functionally similar but not identical phosphatidylinositol kinase homologues in yeast. (yeastgenome.org)
  • The cellular phosphoinositide signaling system, a complicated network of enzymes and phospholipid messengers, is a crucial regulator for many cellular processes [ 4 ]. (biomedcentral.com)
  • Requirement for phosphatidylinositol transfer protein in epidermal growth factor signaling. (nih.gov)
  • In contrast, swapping carboxy-terminal phosphatidylinositol 4,5-bisphosphate (PIP 2 ) binding domains, or the entire C terminus, was without effect on the forskolin response, but the latter conferred responsiveness to arginine-vasopressin (an inhibitory PKC-dependent response). (aspetjournals.org)
  • Enables 1-phosphatidylinositol-3-kinase activity. (nih.gov)
  • The requirement for PI4 kinase activity has also been shown in enterovirus 71 (1). (health-ground.com)
  • Cells were resuspended in 12 ml Dulbecco's modified Eagle medium (DMEM) 2 aliquots were seeded into each well of a 6-well plate and replication was determined by measuring luciferase activity at 4 h 24 h 48 h and 72 h postelectroporation. (colinsbraincancer.com)
  • A large scale purification of Ptdlns 3-kinase was developed to allow gel purification and peptide sequence analysis of both the 85 kDa and 110 kDa proteins. (ucl.ac.uk)
  • 4 In the ARC, there are two distinct neuronal populations: one group of neurons produces the orexigenic neuropeptides neuropeptide Y (NPY) and agouti-related peptide (AgRP), whereas the other subset of neurons expresses the anorexigenic neuropeptides proopiomelanocortin (POMC), and cocaine- and amphetamine-regulated transcript. (nature.com)
  • The net purification of Ptdlns 3-kinase was 650 fold. (ucl.ac.uk)
  • Emerging cell biological functions of phosphatidylinositol 5 phosphate 4 kinase. (ncbs.res.in)
  • Phosphoinositide 3-kinases (PI3-Ks) are an important emerging class of drug targets, but the unique roles of PI3-K isoforms remain poorly defined. (rcsb.org)
  • This is consistent with the reported associations of Ptdlns 3-kinase in vivo, as discussed. (ucl.ac.uk)
  • Phosphatidylinositol-4 kinase III beta and oxysterol-binding protein accumulate unesterified cholesterol on poliovirus-induced membrane structure. (microbiologyresearch.org)
  • Ensembl chr 4:141,748,587. (mcw.edu)
  • PI3P-dependent regulation of cell size and autophagy by phosphatidylinositol 5-phosphate 4-kinase. (ncbs.res.in)