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  • PI3K
  • Phosphatidylinositol-3-kinase (PI3K) pathway deregulation is a common event in human cancer, either through inactivation of the tumor suppressor phosphatase and tensin homologue deleted from chromosome 10 or activating mutations of p110-α. (aacrjournals.org)
  • Phosphatidylinositol 3′-kinase (PI3K) is a key component of multiple signaling pathways, where it typically promotes survival, proliferation, and/or adhesion. (jimmunol.org)
  • One key signal relay molecule in multiple cellular signaling pathways is the enzyme phosphatidylinositol 3′-kinase (PI3K). (jimmunol.org)
  • The products of PI3K, the lipids phosphatidylinositol 3,4-diphosphate and phosphatidylinositol 3,4,5-triphosphate, bind to pleckstrin homology (PH) domains in several signaling intermediates, including the serine/threonine kinases phosphoinositide-dependent kinase 1 and Akt, as well as members of the nonclassical and atypical protein kinase C family ( 3 ). (jimmunol.org)
  • Studies of Jurkat T cells treated with pharmacological inhibitors of PI3K (e.g., wortmannin, Ly294002) or transfected with dominant negative or activated mutants of PI3K suggest that PI3K inhibits TCR-evoked activation of the IL-2P ( 4 , 5 ). (jimmunol.org)
  • Studies of the role of PI3K in TCR responses in primary human ( 4 ) and murine ( 6 , 7 ) T cells are conflicting, with different investigators reporting no effect on, inhibition of, or stimulation of TCR-evoked IL-2 production. (jimmunol.org)
  • This study sought to evaluate primary USC tumors for molecular alterations in epidermal growth factor receptor ( EGFR ) and the recently characterized oncogene PIK3CA , which encodes the catalytic p110-alpha subunit of phosphatidylinositol 3-kinase (PI3K) and thus activates the AKT-mTOR oncogenic pathway. (pubmedcentralcanada.ca)
  • protein
  • The presence of phosphatidylinositol-bis-phosphate (PIP(2)) is required for G protein activation. (nih.gov)
  • Adult rat cardiac myocytes expressed Ci-VSP and the two fluorescent PH-domains within 4 days after gene transfer using the vector integrated into an adenoviral construct.This was paralleled by inhibition of endogenous G protein activated K(+) (GIRK) current.Generally, adenoviral constructs containing self-cleaving 2A-peptide sequences are highly suited for simultaneous transfer of multiple genes in adult cardiac myocytes. (nih.gov)
  • Zusätzlich bieten wir Ihnen SYT1 Proteine (19) und SYT1 Kits (5) und viele weitere Produktgruppen zu diesem Protein an. (antikoerper-online.de)
  • PTEN
  • Despite its importance in regulating the levels of the phosphoinositoltriphosphate PI(3,4,5)P$_{3}$, there is little understanding of how PTEN binds to membranes, is activated and then acts as a phosphatase. (aps.org)
  • In contrast, we find that binding to phosphatidylinositol-4,5-bisphosphate (PI(4,5)P(2)) model membranes is 2.5-fold decreased for the mutant PTEN in comparison to wild-type PTEN. (nih.gov)
  • The structural change previously found for wild-type PTEN upon interaction with PI(4,5)P(2), is absent for H93R PTEN. (nih.gov)
  • A: The H93R mutant PTEN binds PI(4,5)P 2 vesicles (triangles) slightly less strongly than WT PTEN. (nih.gov)
  • C: WT and H93R-PTEN bind to PI(4,5)P 2 + PS vesicles (diamonds) with similar affinities. (nih.gov)
  • insulin
  • The fluorescence micrographs show HEK cells expressing the indicated constructs after 1 h stimulation with 100 nM insulin + 10% serum, 5 µM LY294002, or 10 µM wortmannin. (nih.gov)
  • Although the underlying cause remains unknown, recent studies have clearly demonstrated that insulin resistance plays a critical role in the development of type 2 diabetes ( 2 - 5 ). (diabetesjournals.org)
  • cells
  • C) Maximal current densities with control (1 mM, n = 5 cells) or 10 mM EDTA (n = 3) solutions and with solutions containing inhibitory cations. (nih.gov)
  • Direct exposure of nuclei isolated from undifferentiated ES cells to dynorphin B or U-50,488H, a synthetic κ opioid receptor agonist, time- and dose-dependently activated the transcription of GATA-4 and Nkx-2.5, 2 cardiac lineage-promoting genes. (ahajournals.org)
  • I-J) Electron micrographs of ultrathin cryosections of HeLa cells transfected with E-Syt2-EGFP (I) or E-Syt3-EGFP (J) and double immunogold labeled for EGFP (10 nm gold) to detect E-Syts and for endogenous PDI (5 nm gold) to label the ER lumen. (nih.gov)
  • levels
  • PLC- β 1, PLC- β 3, and PLC- β 4 are distributed in the dorsal root ganglion (DRG), and PLC- β 3 has the highest transcription levels [ 5 ]. (hindawi.com)
  • high affinity
  • Phosphatidylinositol 4, 5-bisphosphate (PIP(2)) profoundly antagonized ATP inhibition of K(ATP) channels expressed from cloned Kir6.2+SUR1 subunits, but also abolished high affinity tolbutamide sensitivity.Conversely, Kir6. (nih.gov)
  • domain
  • Here, we show that the three extended synaptotagmins (E-Syts) are ER proteins that participate in such tethering function via C2 domain-dependent interactions with the PM that require PI(4,5)P2 in the case of E-Syt2 and E-Syt3 and also elevation of cytosolic Ca(2+) in the case of E-Syt1. (nih.gov)
  • drug
  • After 4 weeks of continuous drug administration, the blood glucose, body weight, behavioral indexes, and sciatic nerve conduction velocity were measured. (hindawi.com)