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  • tumor
  • I studied the effect of this compound on FAK-IGF-1R protein interactions, when administered alone or in combination with 5-FU or gemcitabine chemotherapy on cell signaling, viability and apoptosis in human melanoma (A375, C8161, SK-Mel28), esophageal (KYSE 70, 140) and pancreatic (Miapaca-2, Panc-1) cancer cells and on in vivo tumor growth in xenograft mouse models. (ufl.edu)
  • Introduction cell proliferation, differentiation, apoptosis, migration, The identification of a growing list of intracellular extracellular matrix remodeling, immune functions, and kinases that phosphorylate Smad proteins suggests that tumor metastasis. (deepdyve.com)
  • apoptosis
  • It also caused a dose dependent inhibition of cell viability and induction of apoptosis starting at doses of 0.5 microM and 5microM, respectively. (ufl.edu)
  • Smads2/3/4 increased, but Smad7 decreased, MPK38-mediated apoptosis signal-regulating kinase-1 (ASK1)/transforming growth factor-β (TGF-β)/p53 signaling. (deepdyve.com)
  • Apoptosis is regulated by a diverse set of proteins including, but not limited to, the Bcl-2 family. (usf.edu)
  • Unexpectedly, expression of transactivation-deficient p53 variants sensitized p53-null cancer cells to HDACi-mediated Bax-dependent apoptosis, whereas knockdown of endogenous mutant p53 inhibited HDACi-induced apoptosis. (usf.edu)
  • Evaluation of the mechanisms controlling this response led to the discovery of a novel interaction between p53 and Ku70.The association between these two proteins was acetylation independent, but acetylation of p53 could prevent and disrupt the Ku70/Bax complex and enhance apoptosis. (usf.edu)
  • activation
  • MPK38 Given that MPK38 interacts with and phosphorylates and its interacting partner Smad3 have recently been Smad proteins, leading to the activation of TGF-β signal- shown to serve as components of a multi-protein complex ing , we reasoned that Smad proteins would affect MPK38 linking ASK1 and TGF-β signaling pathways, which are activity through direct interaction and phosphorylation. (deepdyve.com)
  • shown
  • This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). (nih.gov)
  • MRNA
  • Previous and current results indicate that insulin treatment not only favors exon-βII inclusion, but also regulates 3'-UTR size in mature mRNA. (usf.edu)
  • whereas
  • Consistent with this, Smads2/3/4 attenuated complex formation between MPK38 and its negative regulator thioredoxin (Trx), whereas Smad7 increased this complex formation. (deepdyve.com)
  • Smads2/3/4 stimulate MPK38- dependent ASK1/TGF-β/p53 signaling pathways, whereas have focused on the regulatory role of Smad phosphor- ylation in the TGF-β signaling pathway. (deepdyve.com)
  • Furthermore, over- on the activity of these interacting kinases in order to expression of Smads2/3/4 improves, whereas Smad7 decipher the molecular interplay between TGF-β and overexpression worsens, obesity-associated metabolic other intracellular signaling pathways. (deepdyve.com)
  • Abstract
  • ABSTRACT Synaptotagmin (Syt) is found ubiquitously throughou t human physiology and plays an important role along with SNARE proteins in vesicle -membrane docking and fusion during Ca2+ induced exocytosis. (auraria.edu)
  • ABSTRACT: Alternative splicing is a major contributing factor to protein diversity. (usf.edu)
  • metabolic
  • When Smads were overexpressed in high-fat diet (HFD)-fed obese mice using an adenoviral delivery system, Smads2/ 3/4 improved, but Smad7 worsened, obesity-associated metabolic parameters and inflammation in a MPK38 phosphorylation-dependent manner. (deepdyve.com)
  • domain
  • Based on GST pull down assay with purified protein fragments of FAK-FERM domain and IGF-1R? (ufl.edu)
  • suggest
  • Our data suggest that the FAK-IGF-1R protein interaction is an important target and disruption of this protein-protein interaction with a small molecule has a potential anti-neoplastic therapeutic effect. (ufl.edu)
  • manner
  • Katayama N , Yamamori S, Fukaya M, Kobayashi S , Watanabe M, Takahashi M and Manabe T (2017) SNAP-25 phosphorylation at Ser187 regulates synaptic facilitation and short-term plasticity in an age-dependent manner. (u-tokyo.ac.jp)
  • system
  • The keystones to this system are the proapoptotic proteins Bax and Bak, which are regulated by their conformation and localization. (usf.edu)
  • effect
  • However, an opposite effect was observed on complex formation between MPK38 and its positive regulator zinc-finger-like protein 9 (ZPR9). (deepdyve.com)
  • size
  • We identified up to five different PKCβII mRNAs that differ only in the 3'-UTR size (all five mature transcripts express the same PKCβII protein). (usf.edu)
  • models
  • The resulting models depict membrane penetration of binding loops 1 and 3 of Syt7 C2A, analogous to Syt1 C2A. (auraria.edu)