- Significantly, photosynthetic capacity (CO2 assimilation at varying light and CO2 and quantum yield of PSII electron transport) per unit leaf area and per leaf dry weight were increased in lines of N. tabacum transformed with the E. coli gene otsA, which encodes trehalose phosphate synthase. (rothamsted.ac.uk)
- Amongst the parameters measured, the main difference between LY-16 and ZD-958 associated with yield penalty was the expression of class II trehalose phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP) genes which were increased due to shading in leaves and ears, particularly in ears of LY-16. (rothamsted.ac.uk)
- Alpha,alpha-trehalose-phosphate synthase 1 n=1 Tax=Coccidioides posadasii str. (uma.es)
- OtsB encodes for a 29.1-kDa trehalose-6-phosphate phosphatase and otsA encodes for a 53.6-kDa trehalose-6-phosphate synthase. (igem.org)
- Together they make up a Saccharomyces cerevisiae trehalose-6-phosphate synthase/phosphatase complex. (igem.org)
- In R. solanacearum , it is known that the OtsA/ OtsB pathway is the dominant trehalose synthesis pathway, and otsA and otsB encode trehalose-6-phosphate (T6P) synthase and T6P phosphatase, respectively. (ppjonline.org)
- However, the specific contributions of trehalose phosphate synthase (TPS) and trehalose phosphate phosphatase (TPP) genes to source- and sink-related traits remain largely unknown. (bvsalud.org)
- OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. (unl.edu)
- Trehalose-6-Phosphate Synthase (TPS) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. (unl.edu)
- Experiments to obtain a mutant in the presence of a second copy of impC containing an active-site mutation, in the presence of porin-like gene of M. smegmatis , or in the absence of inositol 1-phosphate synthase activity, were also unsuccessful. (biomedcentral.com)
- Further, we also found that each otsA and otsB mutant had reduced ability to grow in a medium containing NaCl and sucrose, indicating that trehalose also has an important role in osmotic stress tolerance. (ppjonline.org)
- In contrast, transformation with otsB, which encodes trehalose phosphate phosphatase or Trec, encoding trehalose phosphate hydrolase, produced the opposite effect. (rothamsted.ac.uk)
- Two mitochondrial phosphatases, PP2c63 and Sal2, are required for posttranslational regulation of the TCA cycle in Arabidopsis. (mpg.de)
- Regulation of shoot branching in Arabidopsis by trehalose 6-phosphate. (mpg.de)
- Functional features of TREHALOSE-6-PHOSPHATE SYNTHASE1 - an essential enzyme in Arabidopsis thaliana. (mpg.de)
- Trehalose is pulled right out of the E.coli genome and consists of the genes otsA and otsB. (igem.org)
- We generated otsA and otsB mutant strains and found that these mutant strains reduced the bacterial trehalose concentration and HR induction on eggplant leaves compared to wild-type. (ppjonline.org)
- We report large changes in photosynthesis in Nicotiana tabacum transformed with E. coli genes for the trehalose pathway. (rothamsted.ac.uk)
- Activation of TPSII and TPP genes indicates that the trehalose pathway likely plays a role in ear development under low light and could be a target for yield improvement under such conditions as with other stresses. (rothamsted.ac.uk)
- In T3, genes linked to trehalose metabolism, related to the response to heat stress, dominated. (frontiersin.org)
- We showed that all four genes are expressed, although at different levels, and levels of inositol phosphatase activity did not fall significantly in any of the mutants obtained. (biomedcentral.com)
- In that study, threshold amounts of trehalose - a limiting, produced carbon-resource, controls the emergence and self-organization of cells exhibiting the glycolytic state, serving as a carbon source that fuels glycolysis (Varahan et al. (elifesciences.org)
- The enzyme trehalose-phosphatase (EC 3.1.3.12) catalyzes the reaction α,α-trehalose 6-phosphate + H2O ⇌ {\displaystyle \rightleftharpoons } α,α-trehalose + phosphate This enzyme belongs to the family of hydrolases, specifically those acting on phosphoric monoester bonds. (wikipedia.org)
- The trehalose signaling pathway plays a critical role in adapting C allocation to the environment in crops but its importance in adaptation to low light in field conditions is not known. (rothamsted.ac.uk)
- It is a key enzyme in the trehalose synthesis pathway. (unl.edu)
- The systematic name is α,α-trehalose-6-phosphate phosphohydrolase. (wikipedia.org)
- Other names in common use include trehalose 6-phosphatase, trehalose 6-phosphate phosphatase, and trehalose-6-phosphate phosphohydrolase. (wikipedia.org)
- Inositol is synthesized by conversion of glucose-6-phosphate to inositol-1-phosphate, followed by dephosphorylation by inositol monophosphate phosphatases (IMPases) to form myo -inositol. (biomedcentral.com)
- Further, the effect of the presence of a disaccharide, trehalose, as well as some impurities present in its commercial form, in the subphase of the monolayers was tested. (usp.br)
- The disaccharide trehalose is involved in abiotic and biotic stress tolerance in many organisms. (ppjonline.org)
- Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. (unl.edu)
- Enzyme interaction with phospholipids was followed by changes in Pi-A curves, and their adsorption on DMPA/Zn2+ or DMPA/trehalose LB films using the quartz crystal microbalance technique, QCM. (usp.br)
- Here, we show through combined genetic and chemical modulation of signaling pathways that the sugar small-molecule signal, trehalose-6-phosphate (T6P) regulates root branching through master kinases SNF1-related kinase-1 (SnRK1) and Target of Rapamycin (TOR) and with the involvement of the plant hormone auxin. (bvsalud.org)
- Trehalose 6-phosphate (T6P) signalling regulates carbon use and allocation and is a target to improve crop yields. (bvsalud.org)
- Rising rates of starch degradation during daytime and trehalose 6-phosphate optimize carbon availability. (mpg.de)
- In gluconeogenic cells, aspartate is a carbon source for trehalose production, while in glycolytic cells using trehalose for carbon, aspartate is predominantly a nitrogen source for nucleotide synthesis. (elifesciences.org)
- ABSTRACT Alkaline phosphatases (AP) are unspecific enzymes that hydrolyze phosphate esthers. (usp.br)
- Trehalose-phosphatase 1 n=3 Tax=Physcomitrella patens subsp. (uma.es)
- Role of Trehalose Synthesis in Ralstonia syzygii subsp. (ppjonline.org)
- 2020). The most successful route through which T6P has been genetically modified for yield and resilience so far is to alter trehalose phosphate phosphatase (TPP) activity. (grassroots.tools)
- Some members of this domain family coexist with a C-terminal trehalose phosphatase domain. (unl.edu)
- Auxin, the master regulator of LR formation, impacts this T6P function by transcriptionally down-regulating the T6P-degrader trehalose phosphate phosphatase B in LR cells. (bvsalud.org)
- The Trehalose helps the frozen dehydrated cells to recover back to a functioning hydrated state. (igem.org)
- Together the sugar Trehalose is produced which helps the cell recover from freeze-drying. (igem.org)