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  • pyridoxal
  • This HMM describes a protein related to a number of pyridoxal phosphate-dependent enzymes, and in particular to selenocysteine synthase (SelA), which converts Ser to selenocysteine on its tRNA. (jcvi.org)
  • 2. The α subform contained 2mol of pyridoxal 5′-phosphate. (biochemj.org)
  • 5. The β subform had approximately half the specific enzyme activity of the α subform, and contained an average of one active pyridoxal 5′-phosphate molecule per molecule, which could be removed by glutamate, and another inactive cofactor which could only be removed with NaOH. (biochemj.org)
  • increase
  • In fact, upon stimulation with foskolin, an activator of adenylate cyclase, we observed a clear increase in the level of both PiC mRNA and PiC protein and a two-fold activation of CAT expression. (unibas.it)
  • found
  • While resembling SelA, this protein is found only in species that have a better candidate SelA or else lack the other genes (selB, selC, and selD) required for selenocysteine incorporation. (jcvi.org)
  • By Southwestern experiments two polypeptides (about 100 kDa and 55 KDa, respectively) were found to bind to a region of 25 nucleotides (from -943 to -919 bp). (unibas.it)
  • Interactions
  • Interactions are determined by geometric criteria as described in K. Stierand, M. Rarey (2010), Drawing the PDB: Protein-ligand complexes in two dimensions, ACS Med. (rcsb.org)
  • Srinivasan's researches were broadly on protein evolution with regard to its structure, functions and interactions. (wikipedia.org)
  • Osteonectin is an acidic extracellular matrix glycoprotein that plays a vital role in bone mineralization, cell-matrix interactions, and collagen binding. (wikipedia.org)
  • Sodium phosphate removes calcium from the Ca-paracaseinate cheese complex due to ion exchange interactions, where positive calcium ions bind to the negative phosphate groups. (wikipedia.org)
  • Alkaline phosphatase in E. coli is uncommonly soluble and active within elevated temperature conditions such as 80 °C. Due to the kinetic energy induced by this temperature the weak hydrogen bonds and hydrophobic interactions of common proteins become degraded and therefore coalesce and precipitate. (wikipedia.org)
  • cysteine
  • Osteonectin (ON) also known as secreted protein acidic and rich in cysteine (SPARC) or basement-membrane protein 40 (BM-40) is a protein that in humans is encoded by the SPARC gene. (wikipedia.org)
  • Osteonectin is a 40 kD acidic and cysteine-rich glycoprotein consisting of a single polypeptide chain that can be broken into 4 domains: 1) a Ca++ binding domain near the glutamic acid-rich region at the amino terminus (domain I), 2) a cysteine-rich domain (II), 3) a hydrophilic region (domain III), and 4) an EF hand motif at the carboxy terminus region (domain IV). (wikipedia.org)
  • fatty
  • Byproducts of the enteric bacterias (from carbohydrate plus some proteins metabolism) are the brief chain fatty acidity propionic acidity (PPA) which have the ability to enter blood flow and could alter immune system function and/or exacerbate ASD behaviors. (iftm-pharo2005.org)
  • In addition to its role in glycolysis, TPI is also involved in several additional metabolic biological processes including gluconeogenesis, the pentose phosphate shunt, and fatty acid biosynthesis. (wikipedia.org)
  • structural
  • Deploying computational genomics, he is known to have assisted in identifying similar proteins with shared structural and functional features. (wikipedia.org)
  • The encoded protein does not perform a structural role in lens tissue, and instead it binds thyroid hormone for possible regulatory or developmental roles. (wikipedia.org)
  • homology
  • TIP47 belongs to the peripilin protein family and shares significant homology with the other genes of this family, including perilipin and adipophilin. (wikipedia.org)
  • Sequence
  • In contrast, protein phosphatases (PPs) are the primary effectors of dephosphorylation and can be grouped into three main classes based on sequence, structure and catalytic function. (wikipedia.org)
  • The Walker A and Walker B motifs are protein sequence motifs, known to have highly conserved three-dimensional structures. (wikipedia.org)
  • phosphatases
  • The protein Tyr phosphatase (PTP) super-family forms the second group, and the aspartate-based protein phosphatases the third. (wikipedia.org)
  • Examples of membrane-spanning protein phosphatases containing both active (phosphatase) and inactive (pseudophosphatase) domains linked in tandem are known, conceptually similar to the kinase and pseudokinase domain polypeptide structure of the JAK pseudokinases. (wikipedia.org)
  • 2007). The protein Ser/Thr phosphatases PP1, PP2A and PP2B of the PPP family, together with PP2C of the PPM family, account for the majority of Ser/Thr PP activity in vivo (Barford et al. (wikipedia.org)
  • intracellular
  • Like its mechanistic cousins, IP3 and cyclic adenosine diphosphoribose (Cyclic ADP-ribose), NAADP binds to and opens Ca2+ channels on intracellular organelles, thereby increasing the intracellular Ca2+ concentration which, in turn, modulates sundry cellular processes (see Calcium signalling). (wikipedia.org)
  • The existence of intracellular thyroid hormone binding proteins has been postulated from mathematical modelling of pituitary-thyroid homeostasis. (wikipedia.org)
  • compound
  • Surely, with the strong anionic charge of phosphate groups along with the remainder of the compound they are very much immiscible in the nonpolar region of the bilayer. (wikipedia.org)
  • The solution arises in cleaving the phosphate group away from the compound via ALP. (wikipedia.org)
  • calcium
  • Osteonectin is a glycoprotein in the bone that binds calcium. (wikipedia.org)
  • More specially, chelating salts bind calcium ions to hydrate proteins and create a more uniform spread. (wikipedia.org)
  • Sodium citrate and sodium phosphate are the main emulsifiers used in Easy Cheese to sequester calcium in cheddar cheese. (wikipedia.org)
  • The alpha and beta-casein are kept in place by "colloidal calcium phosphate-mediated cross links" covered with a kappa-casein outer layer. (wikipedia.org)
  • The hydrophilic portion of sodium phosphate removes calcium from the calcium paracaseinate from ion exchange reactions. (wikipedia.org)
  • This action causes "hydration and partial dispersion of the calcium-paracaseinate phosphate network. (wikipedia.org)
  • The phosphate groups of the nucleotide are also coordinated to a divalent cation such as a magnesium, calcium, or manganese(II) ion. (wikipedia.org)
  • homologous
  • It consists of fifteen repeats homologous to the 157-residue CD-M6PR domain, two of which are responsible for binding to M6P. (wikipedia.org)
  • bone
  • The best characterized of these bone proteins is osteonectin. (wikipedia.org)
  • It binds collagen and hydroxyapatite in separate domains, is found in relatively large amounts in immature bone, and promotes mineralization of collagen. (wikipedia.org)
  • genes
  • The logical for looking into genes suffering from TS and 5-FU at both transcriptional and post-transcriptional amounts is usually that TS, furthermore to its crucial enzymatic function, features like a RNA 906-33-2 manufacture binding proteins . (iftm-pharo2005.org)
  • Hence, it might be particularly vital that you systematically investigate various other potential post-transcriptional governed genes via TS proteins. (iftm-pharo2005.org)
  • peptide
  • since such a short peptide does not form an alpha helix, this suggests that it is the nest, rather than being at the N-terminus of a helix, that is the main phosphate binding feature. (wikipedia.org)
  • Human
  • toxin A,9 and protein SpsA.10C12 Interferon- (IFN-),13 TNF-,14 IL-415 and IL-116 can up-regulate the release of SC into the culture supernatant of the human being colonic adenocarcinoma cell range, HT-29. (iftm-pharo2005.org)
  • The translation of human being TS mRNA is usually regulated by its proteins product with a unfavorable autoregulatory system whereby the binding of TS proteins to at least two unique sequences alone TS mRNA leads to translational repression [12, (iftm-pharo2005.org)
  • known
  • He is known for his researches in the fields of computational genomics and protein structure analysis. (wikipedia.org)
  • In 2010, he was promoted as a professor and he heads the Proteins : Structure, Function and Evolutionary Group, popularly known as N. S. Group, of the MBU. (wikipedia.org)
  • Thus
  • Thus an increase in Aβ results in an increase of the AKT/BAD complex, in turn stopping the action of the anti-apoptotic protein Bcl-2, which normally functions to stop cell death, causing accelerated neuron breakdown and the progression of AD. (wikipedia.org)
  • Thus, ADP and beryllium fluoride together tend to bind to ATP sites and inhibit protein action, making it possible to crystallise proteins in the bound state. (wikipedia.org)
  • Alkaline phosphatase in E. coli is located in the periplasmic space and can thus be released using techniques that weaken the cell wall and release the protein. (wikipedia.org)
  • group
  • Narayanaswamy Srinivasan (born 1962) is an Indian molecular biophysicist and a professor and the head of Proteins : Structure, Function and Evolutionary Group (N. S. Group) at the Molecular Biophysics Unit of the Indian Institute of Science. (wikipedia.org)
  • cell
  • Gem and rlipo-E7m/CpG reduced the quantity of programmed cell death protein 1 (PD-1)-expressing antigen-specific cytotoxic T lymphocytes (CTLs) in the regressing tumors. (iftm-pharo2005.org)
  • It might be beneficial to correlate the adjustments in cell morphology that take place on wounding with adjustments in the function and distribution of laminin-binding integrins. (iftm-pharo2005.org)
  • Right here, we examine the system Cinacalcet HCl of epithelial cell motion during wound closure by following mobilization from the actin cytoskeleton and laminin-binding integrins in response to damage of T84 monolayers. (iftm-pharo2005.org)
  • In vascular endothelial cells the binding of S1P to S1PR1 induces migration, proliferation, cell survival and morphogenesis into capillary-like structures. (wikipedia.org)
  • Moreover, the binding of S1P to S1PR1 is implicated in the formation of cell-cell adherens junctions, therefore inhibiting paracellular permeability of solutes and macromolecules. (wikipedia.org)
  • molecular
  • 14 Our results targeted the laminin-binding integrins, 61, 64, and 31, within the molecular equipment in charge of epithelial wound recovery, however the true stage of which these integrins take part in the healing up process is unknown. (iftm-pharo2005.org)
  • residue
  • In this mechanism, both PAPS and the target tyrosine bind to the same active site in the enzyme and are orientated in a way such that a glutamic acid residue acts as a catalytic base on the tyrosine hydroxyl group, an arginine residue acts as a catalytic acid, and serine and lysine residues are used to stabilize the SN2-like intermediate. (wikipedia.org)
  • The deprotonated hydroxyl would attack the sulfonate group, then displace the phosphate group and PAP would be released, along with the sulfotyrosine residue. (wikipedia.org)
  • These domains are joined by residue loops to form a substrate binding cleft, where the glucosyl group acceptor binds. (wikipedia.org)
  • Furthermore, acetylation of a specific lysine residue binds to bromine-containing domains of certain transcription and chromatin regulatory proteins. (wikipedia.org)
  • interact
  • NPs may interact with these proteins in a similar way, and the interaction may lead to beneficial outcomes in vaccine delivery. (scirp.org)
  • Meanwhile, several other proteins interact directly with the oriC sequence to regulate initiation, usually by inhibition. (wikipedia.org)
  • After binding, fructose 6-phosphate will interact with UDP via a hydrogen bond, which lowers the activation energy of the reaction and stabilizes the transition state. (wikipedia.org)
  • Histone H2B isoforms interact differently with other proteins, are found in specific regions of chromatin, have different types and numbers of post-translational modifications, and are more or less stable than regular histone H2B. (wikipedia.org)
  • Interacting proteins with the strongest confidence to interact with C3orf62 include: HAUS augmin-like complex subunit 1 (HAUS-1), Inhibitor of growth protein 5 (ING5), Thioredoxin domain-containing protein 9 (TXNDC9), and MORF4-family associated proteins (MORF4L1, MFRAP1). (wikipedia.org)
  • Nucleic
  • The three main purposes of metabolism are the conversion of food/fuel to energy to run cellular processes, the conversion of food/fuel to building blocks for proteins, lipids, nucleic acids, and some carbohydrates, and the elimination of nitrogenous wastes. (wikipedia.org)
  • Metabolism is usually divided into two categories: catabolism, the breaking down of organic matter for example, the breaking down of glucose to pyruvate, by cellular respiration, and anabolism, the building up of components of cells such as proteins and nucleic acids. (wikipedia.org)
  • Absorbance detection has been available in microplate readers for more than 3 decades, and is used for assays such as ELISA assays, protein and nucleic acid quantification or enzyme activity assays (i.e. in the MTT assay for cell viability). (wikipedia.org)
  • bacteria
  • Although a tungsten-containing xanthine dehydrogenase from bacteria has been found to contain tungsten-molydopterin and also non-protein-bound selenium (thus removing the possibility of selenium in selenocysteine or selenomethionine form), a tungsten-selenium molybdopterin complex has not been definitively described. (wikipedia.org)
  • secretory
  • It is localized to the Golgi apparatus, specifically in the trans-Golgi region, and acts almost exclusively on secretory and plasma membrane proteins. (wikipedia.org)
  • ligand
  • rather, this is the name of the ligand (a pterin) that will bind the active metal. (wikipedia.org)
  • An N-terminal helix folds back, forming a deep basic groove that forms the binding pocket for the Ins(1,4,5)P3 ligand. (wikipedia.org)
  • bacterial
  • Bacterial origins regulate orisome assembly, a nuclei-protein complex assembled on the origin responsible for unwinding the origin and loading all the replication machinery. (wikipedia.org)
  • In E. coli, the direction for orisome assembly are built into a short stretch of nucleotide sequence called as origin of replication (oriC) which contains multiple binding sites for the initiator protein DnaA (a highly homologous protein amongst bacterial kingdom). (wikipedia.org)
  • These glycoproteins include proline rich proteins that allow bacterial adhesion. (wikipedia.org)
  • hydroxyl
  • Since the C-2 hydroxyl group (-OH) is ultimately eliminated, the possibility of having D-glucose-1-phosphate cannot also be ruled out. (wikipedia.org)
  • monophosphate
  • The monophosphate is adenylated (coupled to ADP) in a step that activates the cofactor toward binding Mo or W. These metals are imported as their oxyanions, molybdate, and tungstate. (wikipedia.org)
  • reversible
  • It is involved in the regulation of adenine nucleotide composition within a cell by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. (mybiosource.com)
  • membrane
  • A few other mechanisms in E. coli that variously regulate initiation are DDAH (datA-Dependent DnaA Hydrolysis, which is also regulated by IHF), inhibition of the dnaA gene (by the SeqA protein), and reactivation of DnaA by the lipid membrane. (wikipedia.org)
  • Proteins containing this domain have been found to bind PtdIns(4,5)P2 and PtdIns(1,4,5)P3 suggesting that the domain is a membrane-interacting module. (wikipedia.org)
  • In particular, epsin-1 shows specificity for the membrane glycophospholipid phosphatidylinositol-4,5-bisphosphate, however not all ENTH domains bind to this molecule. (wikipedia.org)
  • Binding causes tubulation of liposomes and in vivo this membrane-binding function is normally coordinated with clathrin polymerisation. (wikipedia.org)
  • It consists of a short cytosolic region that contains the N-terminus of the protein, a single transmembrane region of about 17 amino acids in length, a small stem region of about 40 amino acids in length, and a larger, catalytic region that is located on the luminal side of the membrane. (wikipedia.org)
  • C3orf62 has a KKXX-like motif in the C-terminus meaning C3orf62 may be responsible for retrieval of endoplasmic reticulum (ER) membrane proteins from the Golgi apparatus. (wikipedia.org)
  • recombinant
  • Small volumes of AK1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. (mybiosource.com)
  • We investigated the interaction of PEGylated and un-PEGylated PLGA NPs with Recombinant Human Mannose-Binding Protein (HMBP) in an effort to understand the effect of surface modification on their binding to the protein. (scirp.org)
  • Positive controls were recombinant PINK1 protein and overexpressed PINK1 transfected HEK293 @ 1µg/mL in western blot. (rockland-inc.com)
  • steroids
  • for example, some steroids can beassayed by a radioimmunoassay, competitive protein-binding, gas-liquid chromatography, or indirectly where can i buy doxycycline in singapore by a 17-hydroxycor-ticosteroid assay.figures 5-16 and 5-17 show the results of a comparisonof prednisone tablets using a competitive protein-bindingmethod and a radioimmunoassay, respectively. (clubektmportugal.com)
  • facilitates
  • Epsin binding to membranes facilitates their deformation by insertion of the N-terminal helix into the inner leaflet of the bilayer, pushing the head groups apart. (wikipedia.org)
  • This docking facilitates the recruitment of these proteins to the correct region of the chromosome. (wikipedia.org)
  • Transcription
  • Specifically, in some lower eukaryotes, a histone H2B variant binds to a histone H2A variant called H2AZ, localizes to active genes, and supports transcription in those regions. (wikipedia.org)
  • recruit
  • The DnaC helicase loader then interacts with the DnaA bound to the single-stranded DNA to recruit the DnaB helicase, which will continue to unwind the DNA as the DnaG primase lays down an RNA primer and DNA Polymerase III holoenzyme begins elongation. (wikipedia.org)
  • The main function of proteins containing this domain appears to be to act as accessory clathrin adaptors in endocytosis, epsin is able to recruit and promote clathrin polymerisation on a lipid monolayer, but may have additional roles in signalling and actin regulation. (wikipedia.org)
  • variants
  • Of the four splice variants, two of them are protein coding, one is nonsense meditated decay, and one is a retained intron. (wikipedia.org)
  • sequence
  • The linear sequence of amino acids that is used to make a protein is its primary/secondary/tertiary structure? (cram.com)
  • There are 11 DnaA binding sites/boxes on the E. coli origin of replication out of which three boxes R1, R2 and R4 (which have a highly conserved 9 bp consensus sequence 5' - TTATC/ACACA ) are high affinity DnaA boxes. (wikipedia.org)
  • DMSO
  • Remedies had been daily in a single vision for 7 consecutive times with 5 L of latanoprost-free acidity (100 M dissolved in 1:1000 DMSO in PBS) or 10 mM Y27632 (dissolved in phosphate-buffered saline). (ovarian-cancersymptoms.com)
  • DMSO reductase, the metal is bound to two molybdopterin cofactors. (wikipedia.org)
  • pathway
  • The complement or mannose binding pathway, a part of the pathways in the innate immune systems, is one of the first lines of defense for the host cells that the NPs drug delivery system would encounter. (scirp.org)
  • high
  • During initiation, DnaA bound to high affinity DnaA box R4 donates additional DnaA to the adjacent low affinity site and progressively fill all the low affinity DnaA boxes. (wikipedia.org)
  • prevents
  • GlmY functions as an anti-adaptor, it binds to RapZ (RNase adaptor protein for sRNA GlmZ), this binding prevents RapZ from binding to GlmZ and targeting it for cleavage by RNase E. Argaman L, Hershberg R, Vogel J, et al. (wikipedia.org)
  • forms
  • They bind to DnaA-ADP and DnaA-ATP with equal affinities and are bound by DnaA throughout most of the cell cycle and forms a scaffold on which rest of the orisome assembles. (wikipedia.org)