Phosphatase overexpression cell cycle. Medical search. Frequent questions

A multistage process has been proposed for the evolution of esophageal squamous cell carcinoma (ESCC), in which normal squamous epithelia undergo a series of histological and genetic progression towards noninvasive precursor lesions, i.e., dysplasia (DYS) and carcinoma in situ (CIS), then towards invasive cancer, and, finally towards deep invasion and metastasis to lymph nodes and other organs. (biomedcentral.com)
Nim1-related kinases coordinate cell cycle progression with the organization of the peripheral cytoskeleton in yeast. (microbiologyresearch.org)
This autonomous Cdc14 release oscillator functions at constant cyclin-Cdk levels by titrated introduction of undegradable Clb2, and at cell-cycle-average Clb2 levels given a block of cell cycle progression by actin depolymerization. (rockefeller.edu)
Multivariate analysis revealed that coincident mdm2/p53 overexpression, the presence of distant metastases,and tumor grade were independent predictors for tumor progression. (aacrjournals.org)
PTEN can inhibit cell cycle progression and induce cell death to stimulate angiogenesis [ 6 ]. (aging-us.com)
Y336* inhibits Akt phosphorylation and associates with heterochromatin to similar levels of wild-type Pten, but fails to stabilize Hp1alpha protein and enhances Hp1alpha polyubiquitination, leading to satellite DNA overexpression, failure to suppress cell growth, arrest cycle cycle progression, or inhibit transformation in cultured cells ( PMID: 25946202 ). (jax.org)
proteasome inhibition interferes with cell cycle progression, upregulates tumor suppressors such as p53, and diminishes activation of pro-proliferation pathways such as those controlled by NFB and extracellular signal-regulated kinases (ERKs) [3], [4]. (scienza-under-18.org)
12 The activation of cyclin/CDK complex accelerates cell cycle progression, which are regulated by phosphorylation. (jcpjournal.org)
The Cdc25A dual-specificity phosphatase promotes cell cycle progression by dephosphorylating and activating the cyclin-dependent kinases. (pitt.edu)
Moreover, flowcytometry was applied to analyze the effects of miR-183 on the cell cycle progression. (nature.com)
MiR-183 can exert an oncogenic effect by promoting cell viability, migration, and cell cycle progression. (nature.com)
In the present study, the effect of miR-183 on cell viability, cell cycle progression, and migration was investigated, and the interaction between miR-183 and PTEN in BC cells was examined. (nature.com)
Investigating the mechanism of synthetic lethality, we reveal that CHK1 inhibition in IGF-1R depleted or inhibited cells further downregulated RRM2, reduced dNTP supply and profoundly delayed replication fork progression. (nature.com)
HGF c Met signaling can induce paxillin phosphorylation at its tyrosine residue, which in turn promotes tumor progression by enhancing tumor cell migration and spread.ten Activating c Met mutations are already proven to increase paxillin phosphorylation in SCLC.5 Furthermore, paxillin has become proven to be very expressed, and its gene from time to time amplified or mutated in NSCLC 11. (hormonespathway.com)
PP4IP interacts with PP4 complex, which is affected by DNA damage and cell cycle progression and decreases the dephosphorylational activity of PP4. (molcells.org)
Strict localization of AURKB by CPC means that the kinase which includes >50 substrates (8) phosphorylates the Col4a6 right targets at the correct guidelines in cell routine progression. (pkc-inhibitor.com)
The progression from insulin resistance to type 2 diabetes is caused by the failure of pancreatic β cells to produce sufficient levels of insulin to meet the metabolic demand. (jci.org)
In addition, they play important roles in gene transcription (via activation of mitogen activated protein (MAP) kinase pathways and, in higher eukaryotes, the transcription factor NFκB), generation of reactive oxygen species, apoptosis, and cell-cycle progression. (biomedcentral.com)

To confirm the immunohistochemical results, three proteins, fascin, CK14 and laminin-5γ2, which were overexpressed in ESCC on tissue microarray, were detected in 12 ESCC cell lines by Western blot assay. (biomedcentral.com)
Such proteins regulate cell cycle and DNA stability. (fapesp.br)
In particular, the value of individual protein overexpression as well as combined p53/mdm2 positivity was evaluated because both proteins are functionally connected, and their expression is controlled by an autoregulatory feedback loop. (aacrjournals.org)
The overexpression of mdm2 and p53 proteins was investigated on paraffin-embedded material by using monoclonal antibodies. (aacrjournals.org)
p53 protein is involved in cell cycle control but can be inactivated by binding to mdm2 or viral proteins. (aacrjournals.org)
The ubiquitin-proteasome pathway is an essential quality control mechanism directing degradation of mislocated, misfolded, and damaged proteins, and, by tempering the expression levels Carnosol of specific signaling proteins, it also exerts a level of control over cell physiology [1]. (scienza-under-18.org)
ERK signaling is usually inappropriately activated in a wide array of human cancers, which can be caused by an activating mutation in one of the upstream signaling proteins or through overexpression of growth factors or growth factor receptors [7], [8]. (scienza-under-18.org)
Considering that a host of intracellular proteins are affected by proteasome inhibition, coupled with evidence that knockdown of MKP3 expression enhances growth factor-stimulated ERK phosphorylation in some contexts [13] but not in others [16], led us to question whether or not the diminution of ERK signaling in MG132-treated cells could be attributed solely to upregulation of MKP3 and other DUSPs. (scienza-under-18.org)
Molecules can be transported around a cell by so-called motor proteins that move along a network of filaments called microtubules. (elifesciences.org)
Western blot assessment confirmed that myc tagged human SOD1 proteins were induced by doxycycline in these cell lines. (hormonespathway.com)
E2F proteins are posttranslationally modified concomitantly with a reduction in nuclear binding activity in cells infected with herpes simplex virus 1. (uchicago.edu)
Of the three tegument proteins that package mRNA in herpes simplex virions, one (VP22) transports the mRNA to uninfected cells for expression prior to viral infection. (uchicago.edu)
The US3 protein kinase of herpes simplex virus 1 mediates the posttranslational modification of BAD and prevents BAD-induced programmed cell death in the absence of other viral proteins. (uchicago.edu)

A functional shoot meristem is maintained through a tightly controlled balance between the proliferation of a group of stem cells residing in the center, and the differentiation of their peripheral and basal progeny cells for the formation of organ primordia and other differentiated tissues. (biologists.com)
The RAC serine/threonine-protein kinase (AKT) family of serine/threonine protein kinases, particularly the AKT1 isoform, has been identified abnormally expressed in hepatocellular carcinoma (HCC) cells, and is highly associated with cell behavior, including proliferation, survival, metabolism, and tumorigenesis. (spandidos-publications.com)
The purpose of the present study was to reveal the effects of AKT1 on the survival and proliferation of HCC cells, and to investigate the mechanisms involved. (spandidos-publications.com)
Molecular mechanisms and the influences of different regulation the expression of AKT1 on HCC cell growth, proliferation were determined by western blotting, MTT and colony formation assays, cell cycle and apoptosis were investigated by flow cytometry. (spandidos-publications.com)
The phosphoinositide 3-kinase (PI3K)/RAC seine/threonine-protein kinase (AKT)/mechanistic target of rapamycin (mTOR) signaling pathway is a promising therapeutic target owing to its frequent dysregulation in HCC and the critical functions it has in regulating cell survival, proliferation, apoptosis, migration and angiogenesis through phosphorylation of distinct protein substrates ( 6 , 7 ). (spandidos-publications.com)
AKT is a key molecule in the PI3K/AKT/mTOR signaling pathway, which has been shown to serve notable functions in the regulation of cell viability and to be closely associated with a variety of disorders caused by dysfunctional cellular proliferation ( 8 , 9 ). (spandidos-publications.com)
However, the precise molecular mechanisms by which AKT1 promotes cell proliferation and regulates apoptosis ( 18 , 19 ) remain largely unclear. (spandidos-publications.com)
High expression of activated AKT can be detected in HCC, and AKT may promote cell proliferation and regulation of cells apoptosis in HCC ( 20 , 21 ). (spandidos-publications.com)
Subsequent mechanism investigations revealed that AKT1 served a notable function in cell proliferation and anti-apoptosis by directly regulating the expression of phosphatase and tensin homolog (PTEN) and Notch1. (spandidos-publications.com)
SOX9 promotes nasopharyngeal carcinoma cell proliferation, migration and invasion through BMP2 and mTOR signaling. (cancerindex.org)
Overexpression of SOX9 promoted NPC cell proliferation, migration and invasion. (cancerindex.org)
Further, the results of miR-4755-5p mimic transfection confirmed that under the action of NaF, miR-4755-5p overexpression reduced Cyclin D1 protein expression within osteoblasts and further inhibited cell proliferation and activation. (fluoridealert.org)
Because colorectal cancer's migration and invasion are one of main factors to the severity of the disease, exploring the molecular mechanisms of proliferation, migration and invasion of CRC cells will contribute to the development of targeted therapy [ 5 ]. (aging-us.com)
Inhibitory effect of EESR on cancer cell growth and proliferation was determined by water-soluble tetrazolium salt assay. (jcpjournal.org)
EESR possessing antioxidant activity efficiently inhibits proliferation of HT29 cells by inducing both cell cycle arrest and apoptosis. (jcpjournal.org)
6 , 7 Therefore, the inhibition of cancer cell proliferation by inducing cell cycle arrest and apoptosis is being an important preventive strategy against cancer. (jcpjournal.org)
After the cells were validated, we investigated the effect of Fra-1 overexpression and deletion on CGL1 growth and proliferation we performed clonogenic survival assay with 0Gy, 0.5Gy, 1Gy, 2Gy, 4Gy, 6Gy, and 9Gy of X-radiation. (beyondsciences.org)
Further, high NEK2 expression promoted proliferation, colony formation, migration and invasion of HCC cell lines. (oncotarget.com)
While knockout circIFT80 in HT29 cell or SW480 cells, the proliferation, and migration of the cells were inhibited, the cell cycle was arrested in G2/M phase, and the cell apoptosis was increased. (hindawi.com)
For example, circRNA_000166 can function as an oncogene by promoting the proliferation of CRC cells and by limiting apoptosis. (hindawi.com)
β -Catenin is a transcription factor that plays an important role in cell proliferation, cellular differentiation, apoptosis, and metastasis [ 10 ]. (hindawi.com)

The activation of AKT1 suppressed the expression of phosphatase and tensin homolog and increased the activation of Notch1. (spandidos-publications.com)
High-grade astrocytomas are astrocyte-derived gliomas, which pathogenesis includes overexpression of the epidermal growth factor receptor (EGFR) and loss of p53 and phosphatase and tensin homolog deleted on chromosome 10 (PTEN) protein function. (fapesp.br)
PTEN (phosphatase and tensin homolog deleted on chromosome 10) belongs to a cancer suppressor gene (one of the protein phosphatases). (aging-us.com)
Phosphatase and tensin homolog (PTEN) is well recognized as a tumor suppressor gene. (nature.com)

Overexposure to UV in normal skin will induce p53-dependent apoptosis, which may serve to protect the skin from damaged cells. (medscape.com)
Promoting PTEN expression in CRC cell lines will stop the activation of the phosphatidylinositol-3-kinase ( PI3K )/sinkserine/threonine kinase ( Akt ) signaling pathway, which affects CRC cell growth, promotes apoptosis, and improves sensitivity to targeted therapy, immunotherapy, and conventional therapy. (aging-us.com)
To investigate the mechanism of EESR-mediated cytotoxicity, HT29 cells were treated with various concentrations of EESR and the induction of cell cycle arrest and apoptosis was analyzed by flow cytometry, 4,6-diamidino-2-phenylindole staining, and Western blot analysis. (jcpjournal.org)
Cancer cells can grow unusually via limitless number of cell division and can avoid apoptosis. (jcpjournal.org)
Also, to determine the contribution of Fra-1 in any cellular pathways within the CGL1 cells we ran RT-qPCR using a gene arrays of families that include DNA damage response, apoptosis, cell cycle regulation, and AP-1 complex. (beyondsciences.org)
BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. (thermofisher.com)
Protein phosphatase 4 (PP4) is a crucial protein complex that plays an important role in DNA damage response (DDR), including DNA repair, cell cycle arrest and apoptosis. (molcells.org)
To prevent these detrimental consequences, eukaryotic cells have evolved an elaborate and complex system, so called DNA damage response (DDR) comprising of DNA DSB repair, cell cycle arrest, apoptosis, and transcriptional regulation ( Jackson and Bartek, 2009 ). (molcells.org)
These findings suggest that CHOP is a fundamental factor that links protein misfolding in the ER to oxidative stress and apoptosis in β cells under conditions of increased insulin demand. (jci.org)
Bcl-2 blocks a caspase-dependent pathway of apoptosis activated by herpes simplex virus 1 infection in HEp-2 cells. (uchicago.edu)
Wild-type herpes simplex virus 1 blocks programmed cell death and release of cytochrome c but not the translocation of mitochondrial apoptosis-inducing factor to the nuclei of human embryonic lung fibroblasts. (uchicago.edu)

This domain can be found in dual specificity phosphatases. (embl.de)

This phosphatase has been shown to dephosphorylate cyclin-dependent kinases (CDKs), and thus may be involved in cell cycle control. (wikipedia.org)
Polo kinases execute multiple roles during cell division. (kent.ac.uk)
Disturbed CPC function can also be due to overexpression of CPC subunits and by deregulation of its regulatory JNJ-26481585 kinases and phosphatases. (pkc-inhibitor.com)
Posttranslational processing of infected cell protein 22 mediated by viral protein kinases is sensitive to amino acid substitutions at distant sites and can be cell-type specific. (uchicago.edu)
They regulate signalling though inhibition of phosphatases and induction of the activity of Stress Activated Protein Kinases, which themselves modulate feedbacks to insulin signalling and FOXO. (biomedcentral.com)
The insulin receptor (IR) and JAK1 tyrosine kinases and STAT3 can serve as direct substrates for the T-cell protein tyrosine phosphatase (TCPTP). (diabetesjournals.org)

The alkaline phosphatase (ALP) activity and bone Gla protein (BGP) content were detected by micronutrient enzymes standard method and ELISA. (fluoridealert.org)
And we investigated HeLa carcinogenesis cellular marker using the alkaline phosphatase assay. (beyondsciences.org)
Alkaline phosphatase assay showed that alkaline phosphatase is about 1.3 fold increased within CGL1FRA-1 knockout cells compared to the control CGL1Cas9 cells, while it is about 0.7 decreased compared to the control CGL1dCas9 cells, however the results do not show any statistical significant. (beyondsciences.org)

The CLAVATA (CLV)signaling pathway, including the CLV1 receptor-kinase, promotes the differentiation of stem cells by limiting the WUS expression domain,yet the mechanism of CLV signaling is largely unknown. (biologists.com)
The results of Western blot illustrated that KIF18A could forward the migration and invasion of CRC cells, and inhibit PTEN , which promoted the activation of PI3K/Akt signaling pathway, thus bringing about the expression of MMP2 and MMP9 . (aging-us.com)
Therefore, targeting drugs molecules in the PTEN signaling pathway may be another strategy to suppress tumor cells by inhibiting tumor activity [ 7 , 8 ]. (aging-us.com)

Its catalytic phosphatase active center is structurally similar to phosphatase, so it is named PTEN . (aging-us.com)

Second, growth inhibition by SigD R468A was mediated by a cell cycle arrest in G2 dependent on the Swe1 morphogenetic checkpoint, but SigD-induced growth inhibition was cell cycle independent. (microbiologyresearch.org)
Co-inhibition of IGF and CHK1 caused synergistic suppression of cell viability, cell survival and tumour growth in 2D cell culture, 3D spheroid cultures and in vivo. (nature.com)
Both overexpression and depletion of PP4IP impairs DSB repairs and sensitizes cells to genotoxic stress, suggesting timely inhibition of PP4 to be indispensable for cells in responding to DNA damage. (molcells.org)

In budding yeast, the phosphatase Cdc14 is released from nucleolus to promote mitotic exit (ME). (rockefeller.edu)
Esp1 contributes to Cdc14 release and ME kinetics mainly through cohesin cleavage: the Esp1 requirement can be largely bypassed if cells are provided Esp1-independent means of separating sister chromatids. (rockefeller.edu)
Its mechanism and regulation of its frequency by Clb2-Cdk, suggest the hypothesis that intrinsically autonomous Cdc14 release cycles are locked at once per cell cycle through entrainment by the cyclin-Cdk oscillator. (rockefeller.edu)

Liu Y , Sun M , Zhang B , Zhao W , . KIF18A improves migration and invasion of colorectal cancer (CRC) cells through inhibiting PTEN signaling. (aging-us.com)
Here, we show that nuclear PTEN, independent of its phosphatase activity, is essential for maintaining heterochromatin structure. (jax.org)
Depletion of PTEN leads to loss of heterochromatic foci, decreased chromatin compaction, overexpression of heterochromatic genes, and reduced protein stability of heterochromatin protein 1 α. (jax.org)
Through qRT-PCR analysis, miR-183 and PTEN mRNA levels in breast cancer cell lines were measured. (nature.com)
PTEN insufficiency promotes the PI3K/AKT path, which is a vital signal for the survival of cancer cells. (nature.com)

The PPS1 gene of Saccharomyces cerevisiae codes for a dual specificity protein phosphatase with a role in the DNA synthesis phase of the cell cycle. (embl.de)

In addition, the scratch wound assay and Transwell assay were conducted to detect the effect of KIF18A on the migration and invasion of CRC cells. (aging-us.com)
Clonogenic survival assay between CGL1, CGL1dCas9, CGL1Cas9 and FRA-1 overexpression and knockout cells did not show any statistically significant when exposed to 0.5Gy, 1Gy, 2Gy, 4Gy, 6Gy, and 9Gy radiation. (beyondsciences.org)
To determine the impacts of miR-183 on cell viability, the MTT assay was used. (nature.com)
To detect the effects of miR-183 on the migration of BC cell lines, wound healing was used along with a Trans-well migration assay. (nature.com)

PP2C family members are known to be negative regulators of cell stress response pathways. (wikipedia.org)
The mechanisms of radioresistance are still poorly understood, despite it has been suggested that miRNAs play an important role in cell signaling pathways. (frontiersin.org)
Indeed, it has been shown that miRNAs play an important role in gene expression, mainly when associated with the monitoring of several cell and metabolic pathways, being also an essential component of the gene silencing machinery in most eukaryotic organisms ( 4 , 8 ). (frontiersin.org)
While Cdc25A downregulation in response to genotoxic stress occurs through defined signal transduction pathways, regulation of Cdc25A protein levels in non-stressed cells is poorly understood. (pitt.edu)
The results of our studies revealed that Cdc25A protein half-life in non-stressed interphase cells is regulated, in part, by Cdk2 kinase activity, and that Cdk2 does not regulate Cdc25A turnover by affecting several known signal transduction pathways that control Cdc25A protein stability. (pitt.edu)
DESCRIPTION: The overall goal of the principal investigator's research program is to understand the role of protein phosphatases in signal transduction pathways. (proquest.com)
This project will increase the scientific knowledge about the cellular effects of Fra-1 protein and its contribution to cellular transformation from a non-malignant cell into a malignant cells, investigate the effect of activator protein complex function in CGL1 and determine any potential cellular pathways for carcinogenesis development. (beyondsciences.org)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)

Biochemical and genetic studies have provided evidence that PP1 is involved in several cell functions, including glycogen metabolism, calcium transport, muscle contraction, gene expression, cell cycle and cell growth. (proquest.com)
The major interest of our research centers on structure-function relationship and physiological role of type 1 serine/threonine protein phosphatases (PP1) with a particular emphasis on glycogen metabolism and hence diabetes and obesity. (proquest.com)

In this present study, we found that circIFT80 was abnormally overexpression in colorectal cancer tissues and tumor cells. (hindawi.com)

Gene Ontology (GO) enrichment analysis of these genes identified an overrepresentation of genes related to the cell cycle. (biomedcentral.com)
We discovered an unprecedented burst of differential expression of cell cycle related genes at one particular point during transition to flowering. (biomedcentral.com)
We published a whole transcriptome study between CGL1, GIM, and CON cells, and identified 1067 differentially expressed genes (DEGs) between the GIM and CON cell lines. (beyondsciences.org)
Therefore, we hypothesize that chromosome 11 may contain potential tumour suppressor genes that prevent radiation induced tumorigenesis in the CGL1 cell model. (beyondsciences.org)
The focus of the research conducted in this laboratory is on the mechanism by which herpes simplex virus with less than 100 genes takes over a human cell with more than 20,000 genes in both lytic and latent infection. (uchicago.edu)

Mutation of the catalytic cysteinyl residue in the active phosphatase domain reduced the in vitro activity of the mutant protein to less than 0.5% of wild type activity, while mutation of the corresponding cysteinyl residue of the inactive phosphatase-like domain had no effect on in vitro activity. (embl.de)
The amino-terminal non-catalytic region of Salmonella typhimurium SigD affects actin organization in yeast and mammalian cells. (microbiologyresearch.org)
The objectives of this project are to understand the molecular basis of the interaction between catalytic and regulatory components, to elucidate the regulatory mechanisms and to define the role in specific cell functions of two forms of type 1 holoenzyme: the ATP-Mg-dependent and the glycogen sarcoplasmic reticulum-associated protein phosphatases. (proquest.com)

Cdc25B was extensively phosphorylated during MII arrest with an increase in the phosphatase activity toward Cdk1. (molcells.org)
Tyrosine specific protein phosphatases (PTPases) contain two conserved cysteines, the second one has been shown to be absolutely required for activity. (embl.de)
It is concluded that the Salmonella SigD protein deprived of its phosphatase activity is able to disrupt yeast morphogenesis by interfering with Cdc42 function, opening the possibility that the SigD N-terminal region might directly modulate small GTPases from the host during infection. (microbiologyresearch.org)
In vitro GAP activity towards RhoA, Rac1 and Cdc42 is not a prerequisite for YopE induced HeLa cell cytotoxicity. (microbiologyresearch.org)
Here we show that primary myoblasts from lipin-1-deficient patients exhibit a dramatic decrease in LPIN1 expression and phosphatidic acid phosphatase 1 activity, and a significant accumulation of lipid droplets (LD). (nih.gov)
We demonstrated that overexpression of ACACB was associated with free fatty acid accumulation in patients' myoblasts whereas malonyl-carnitine (as a measure of malonyl-CoA) and CPT1 activity were in the normal range in basal conditions accordingly to the normal daily activity reported by the patients. (nih.gov)
For a given level of MEK activation, ERK phosphorylation is reduced, consistent with the proposed upregulation of ERK phosphatase activity, Carnosol but maximal MEK activation is also diminished. (scienza-under-18.org)
The objective of this study is to evaluate the antioxidant and anticancer effects of ethanol extract of S. rufopilosa (EESR) and to determine the molecular mechanism of its anticancer activity in human colon carcinoma HT29 cells. (jcpjournal.org)
EESR showed significant antioxidant activity and inhibitory effect on HT29 cell growth in a dose-dependent manner. (jcpjournal.org)
The functional importance of PP4 in cells is evident by the need of tight regulation of its activity required to maintain cell health. (molcells.org)
The infected cell protein 0 of herpes simplex virus 1 dynamically interacts with proteasomes, binds and activates the cdc34 E2 ubiquitin-conjugating enzyme, and possesses in vitro E3 ubiquitin ligase activity. (uchicago.edu)

Furthermore, the data of the present study indicated that B‑cell lymphoma 2 and cyclin D1 is involved in the regulation of AKT1 expression. (spandidos-publications.com)
This concept incorporates autonomous cell cycle oscillators previously reported into a coherent cell cycle control by cyclin-Cdk oscillation, therefore, may have broad implications for the structure and evolution of eukaryotic cell cycle. (rockefeller.edu)
EESR induced cell cycle arrest at G2/M phase in a dose-dependent manner by modulating cyclin B, cyclin-dependent kinase 1 (CDK1), and CDK inhibitor p21 expression. (jcpjournal.org)
8 , 9 Cell cycle program is regulated by cyclin/cyclin-dependent kinase (CDK) complexes. (jcpjournal.org)
Overexpression of cyclin D1 protein, an important cell-cycle regulator, has been observed in hairy cell leukemia and may play a role in the molecular pathogenesis of the disease. (medscape.com)

Alterations to physiologic Cdc25A regulatory mechanisms could be sufficient to result in oncogenic overexpression of this cell cycle regulatory protein. (pitt.edu)
The ATP-dependent phosphatase is a cytosolic enzyme and contains inhibitor-2 as the regulatory subunit, whereas the glycogen SR- associated form interacts with both membranes and glycogen via its regulatory component RGL. (proquest.com)
In addition, the control of the phosphatases will be probed by overexpressing active or dominant negative versions of putative upstream regulatory elements such as those in the MAP kinaseERK cascades. (proquest.com)
An early regulatory function required in a cell type-dependent manner is expressed by the genomic but not the cDNA copy of the herpes simplex virus 1 gene encoding infected cell protein 0. (uchicago.edu)

Previously, we have shown that mutations in two protein phosphatases, POLTERGEIST (POL) and PLL1,partially suppress clv mutant phenotypes. (biologists.com)

Here, we identified a novel PP4 inhibitor, protein phosphatase 4 inhibitory protein (PP4IP) and elucidated its cellular functions. (molcells.org)

It is a necessity of cell division and plays a role by regulating microtubule dynamics and chromosome aggregation [ 12 ]. (aging-us.com)
Similarly, removal of chromosome 11 from CON cells increased transformation frequency. (beyondsciences.org)
The FOSL1 gene, located on chromosome 11, is down-regulated in tumorigenic cells. (beyondsciences.org)

They were "early" corresponding to mild and moderate DYS with overexpression of fascin, FADD and CDC25B and underexpression of Fas, caspase 8, CK4 and annexin I, "intermediate" to severe DYS and CIS with overexpression of FADD and CK14, and "late" to invasive lesions (ESCC) and to advanced pTNM stage ESCC lesions with overexpression of CK14, laminin-5γ2 and SPARC. (biomedcentral.com)
Conversely, overexpression of Cdc25B inhibited Ca2+-mediated release from MII arrest. (molcells.org)

Protein phosphatase 1B is an enzyme that in humans is encoded by the PPM1B gene. (wikipedia.org)
The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. (wikipedia.org)
Although cDNA microarray has been successfully used to explore the gene expression in ESCC, it is difficult to be used in the precursor lesions due to the difficulty to obtain the dysplastic cells accurately. (biomedcentral.com)
A screen for suppressors of this growth arrest identified the RAS2 gene as a multicopy suppressor of the PPS1p overexpression arrest. (embl.de)
The protein encoded by this gene, together with spectrin and actin, constitute the red cell membrane cytoskeletal network. (cancerindex.org)
Using CRISPR/dCas9 and Cas9 gene editing, CGL1 cells were infected with an in-house produced FRA-1 overexpression and FRA-1 knockout lentivirus. (beyondsciences.org)
Dose-dependent and independent temporal patterns of gene responses to ionizing radiation in normal and tumor cells and tumor xenografts. (uchicago.edu)
Hairy cell leukemia is recognized as a clonal B-cell malignancy, as identified by immunoglobulin gene rearrangements that result in a phenotype B-cell expression of surface antigens. (medscape.com)

Whereas p53 and mdm2 positivity correlated significantly ( P = 0.00004), no correlation could be found between mdm2 protein overexpression and tumor stage, lymph node involvement, and presence of distant metastases. (aacrjournals.org)
Accumulation of hairy cells in the bone marrow, liver, and spleen, with very little lymph node involvement, is characteristic of hairy cell leukemia. (medscape.com)

Recently, new DNA scanning techniques using whole-exome sequencing identified 5 missense somatic clonal mutations, including a heterozygous mutation in BRAF that results in the BRAF V600E variant protein, which is oncogenic for other tumors as well, was identified in all hairy cell leukemia patients and may be responsible for the pathogenesis, diagnosis, and targeted therapy of hairy cell leukemia. (medscape.com)

Requirement of Cdc42 for Salmonella typhimurium -induced cytoskeletal reorganization and nuclear responses in cultured cells. (microbiologyresearch.org)
Our results indicate that mdm2/p53 co-overexpression, nuclear grade, and preoperative presence of distant metastasis are independent predictors for poor survival. (aacrjournals.org)

Cell viability was detected by CCK-8 method. (fluoridealert.org)

Western blotting and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) to evaluate the expression levels of AKT1 in HCC SMMC‑7721 cell line. (spandidos-publications.com)
First, expression of SigD R468A caused accumulation of large unbudded cells and loss of septin organization, while SigD expression caused none of these effects. (microbiologyresearch.org)
The present study was designed to analyze the expression of p53 and mdm2 in clear cell renal cell carcinoma with special emphasis on their association with tumor grade and clinical outcome. (aacrjournals.org)
Accordingly, cells treated with MG132 or other proteasome inhibitors exhibit higher expression of MKP3/DUSP6, an ERK1/2-specific DUSP, accompanied by lower levels of ERK phosphorylation stimulated by growth factors [12]C[14]. (scienza-under-18.org)
Tumor xenograft data from Balb/c nude mice demonstrated that HCC cells with high NEK2 expression formed larger tumors than those with low NEK2 expression. (oncotarget.com)
c Abl activation attributable to mutant SOD1 in NSC 34 cells We then investigated no matter whether overexpression of mutant SOD1s influenced the expression of c Abl. (hormonespathway.com)
Western blot assessment revealed that the expression of c Abl was better in cells expressing mutant SOD1s than cells expressing wild variety SOD1. (hormonespathway.com)
This pattern probably results from the expression of the integrin receptor alpha4-beta1 by the hairy cells and the interaction of the receptor with the vascular adhesion molecule-1 (VCAM-1) found in splenic and hepatic endothelia, bone marrow, and splenic stroma. (medscape.com)

The Core's primary effort is dedicated to loss-of-function screening via RNAi and CRISPR-CAS9 technologies although other services such as micro-RNA (miR) screening and cell population barcoding for clonal analysis are also available. (sbpdiscovery.org)
In addition to screens, the Core produces CRISPR-engineered cell lines to serve as models for screening or to aid the follow-up process, and can provide reagents tailored to the needs of each project such as sh/siRNAs, ORF clones, CRISPR reagent aliquots. (sbpdiscovery.org)
PP4IP-knockout cells were generated using the CRISPR/Cas9 system, and the phosphorylation status of PP4 substrates (H2AX, KAP1, and RPA2) was analyzed. (molcells.org)

It has been reported that overexpression of AKT is associated with decreased disease-free survival rates and development of primary carcinomas of the prostate, breast and ovary ( 12 , 13 ). (spandidos-publications.com)
AKT1 also has a crucial effect on cell survival ( 14 - 17 ). (spandidos-publications.com)
In univariate analysis, there was a statistically significant correlation between p53 and mdm2 overexpression in the same tumor and poor survival( P = 0.00179). (aacrjournals.org)
In both genetic and diet-induced models of insulin resistance, CHOP deficiency improved β cell ultrastructure and promoted cell survival. (jci.org)

Our findings suggest that POL and PLL1 are central players in regulating the balance between stem-cell maintenance and differentiation, and are the closest known factors to WUS regulation in the shoot meristem. (biologists.com)
In the present study, up-regulation of SOX9 was observed in both NPC tissues and different NPC cells. (cancerindex.org)
As main findings, 23 miRNAs were already identified as being involved in genetic regulation of PCa cell response to RT. (frontiersin.org)
equally central to the regulation of cell signaling. (scienza-under-18.org)
The purpose of this thesis was to examine the physiological regulation of Cdc25A protein levels in human tumor cells. (pitt.edu)
The chromosomal passenger complex (CPC) plays a pivotal role in the regulation of cell division. (pkc-inhibitor.com)

Here we investigated whether polarized monolayers of intestinal epithelial cells might regulate inflammatory responses by secreting IL-8 in a vectorial fashion (i.e. apical versus basolateral) depending on the location of the TLR stimulus. (biomedcentral.com)
First, given evidence that IGFs regulate the response to IR, we also found evidence that IGF-1R depletion induced endogenous DNA lesions marked by γH2AX foci in prostate cancer cells [ 10 ]. (nature.com)

Squamous cell carcinomas that arise in association with actinic keratosis typically have a low risk for metastasis. (medscape.com)
A cohort of 97 clear cell renal cell carcinomas was analyzed. (aacrjournals.org)

CXCR1 is expressed in the human IEC line Caco-2 BBE [ 3 ], which is in agreement with our own microarray data using Caco-2 BBE cell mRNA (see below). (biomedcentral.com)

Dietary sugars trigger the production of insulin or insulin-like peptides which are sensed by Insulin receptors at the cell surface and initiate a kinase cascade, leading to the activation of the downstream kinase Akt (PKB), the translocation of GLUT4 glucose transporters to the cell surface, and ultimately glucose uptake. (biomedcentral.com)

The authors have previously reported that the Salmonella typhimurium SigD protein, a phosphatidylinositol phosphatase involved in invasion of the host cell, inhibits yeast growth, presumably by depleting an essential pool of phosphatidylinositol 4,5- bis phosphate, and also that a catalytically inactive version, SigD R468A , was able to arrest growth by a different mechanism that involved disruption of the actin cytoskeleton. (microbiologyresearch.org)
The abnormal cell growth is one of the critical characteristics of cancer for invasion or spreading out to other parts of the body. (jcpjournal.org)

In mammalian cells, as many as twenty types of protein have been reported to bind to activated Cdc42 [ 2 ]. (biomedcentral.com)

[ 4 , 5 ] UV radiation acts as an initiator and promoter of carcinogenesis in actinic keratosis and squamous cell carcinoma. (medscape.com)
These individual apoptotic keratinocytes are often referred to as "sunburn" cells and are seen histologically in the epidermis of skin overexposed to sunlight or UV radiation. (medscape.com)
Some drugs may act as photosensitizers, increasing susceptibility to UV radiation, whereas others may increase the risk for squamous cell carcinoma regardless of exposure to UV radiation. (medscape.com)
One of the treatments applied in cancer is radiotherapy (RT), a therapeutic modality that uses ionizing radiation to induce damage in unwanted cells. (frontiersin.org)
The main goal of RT consists in delivering a precise dose of radiation in a target volume, such as tumor, promoting the tumor cells eradication with as minimal damage as possible in surrounding normal tissues ( 13 ). (frontiersin.org)
Irradiation of CGL1 cells with 7Gy of gamma radiation produced tumorigenic segregates called gamma induced mutants (GIM), and non-tumorigenic cells called gamma irradiated control (CON). (beyondsciences.org)
We hypothesize that deletion of FOSL1 in CGL1 promotes radiation induced transformation frequency, while overexpression will decrease the transformation frequency. (beyondsciences.org)

DNA Cell Biol. (cancerindex.org)

The development of esophageal squamous cell carcinoma (ESCC) progresses a multistage process, collectively known as precursor lesions, also called dysplasia (DYS) and carcinoma in situ (CIS), subsequent invasive lesions and final metastasis. (biomedcentral.com)
Squamous cell carcinoma is the most common histological type of esophageal cancer in China. (biomedcentral.com)
This condition is related to squamous cell carcinoma of the skin and is often described as a precursor or early form of squamous cell carcinoma in situ, although most actinic keratoses will not progress to invasive squamous cell carcinoma. (medscape.com)
Solid organ transplant patients are at increased risk for actinic keratosis and squamous cell carcinoma. (medscape.com)

These results show that IL-8 induces autocrine signalling via an apical CXCR1 in Caco-2 BBE intestinal epithelial cells and that this receptor is also expressed on the apical surface of differentiated human intestinal epithelial cells in vivo , suggesting an autocrine function for IL-8 secreted in the lumen. (biomedcentral.com)
Intestinal epithelial cells (IECs) play a key role in the inflammatory response to colonizing or invading microorganisms via the secretion of interleukin-8 (IL-8). (biomedcentral.com)
Modulation of chloride secretory responses and barrier function of intestinal epithelial cells by the Salmonella effector protein SigD. (microbiologyresearch.org)

These effects resulted in significant accumulation of unreplicated single-stranded DNA and increased cell death, indicative of replication catastrophe. (nature.com)

In a screen for suppressors of SigD R468A -induced growth arrest by overexpression of a yeast cDNA library, the Cdc42 GTPase was isolated. (microbiologyresearch.org)

2021). The antiandrogen enzalutamide downregulates TMPRSS2 and reduces cellular entry of SARS-CoV-2 in human lung cells . (essex.ac.uk)

Anatomy23

Organisms12

Diseases4

Chemicals and Drugs122

Analytical, Diagnostic and Therapeutic Techniques and Equipment22

Phenomena and Processes69

Disciplines and Occupations1

Information Science3

Health Care1

Progression18

Proteins13

Proliferation21

Tensin homolog4

Apoptosis11

Dual specificity phosphatases1

Kinases6

Alkaline phosphatase3

Signaling pathway3

Structurally similar1

Inhibition3

Cdc143

PTEN5

Saccharomyces1

Assay4

Pathways8

Metabolism2

Colorectal cancer1

Genes5

Catalytic3

Activity11

Cyclin5

Regulatory4

Suppress1

Inhibitor1

Chromosome3

CDC25B2

Gene8

Lymph node involvement2

Oncogenic1

Nuclear2

Viability1

Expression8

CRISPR3

Survival4

Regulation6

Regulate2

Carcinomas2

MRNA1

Receptors1

Invasion2

Mammalian1

Radiation7

Biol1

Squamous cell carc4

Intestinal epithelial cells3

Accumulation1

Yeast1

Cellular1