AlkalineAcid PhosphataseDual-Specificity PhosphataseStriatal-enriched protein tyrosine phEnzymesSubstrateKinases and phosphatasesPhosphateTyrosine PhosphatasePhospholipid PhosphatasePhosphatidate phosphatasePTPsInhibitionSerineSignal TransductionReagentsColorimetricPhosphorylationNegative regulatorProteinsRegulateMutationsHumanPP2AActivityCellsAffinityResiduesSubstratesTypeCellularDrugsCell
Alkaline29
- What is an Alkaline Phosphatase Test? (medlineplus.gov)
- An alkaline phosphatase (ALP) test measures the amount of ALP in your blood. (medlineplus.gov)
- But an alkaline phosphatase test alone can't identify the source of ALP in your blood, so other tests are usually needed to make a diagnosis. (medlineplus.gov)
- An alkaline phosphatase test is often used to screen for or help diagnose diseases of the liver or bones. (medlineplus.gov)
- Why do I need an alkaline phosphatase test? (medlineplus.gov)
- Your health care provider may order an alkaline phosphatase test as part of a routine checkup. (medlineplus.gov)
- An alkaline phosphatase test may also be ordered if you have symptoms of liver damage or a bone disorder. (medlineplus.gov)
- An alkaline phosphatase test is a type of blood test. (medlineplus.gov)
- Preparations for an alkaline phosphatase test depend on the lab doing the test. (medlineplus.gov)
- High alkaline phosphatase (ALP) levels may be a sign of a liver problem or a bone disorder. (medlineplus.gov)
- If alkaline phosphatase levels are high and the results of liver tests are normal, the problem may be a bone disorder, such as Paget's disease of bone . (medlineplus.gov)
- Tissue-nonspecific alkaline phosphatase (TNAP) recently emerged as a promising therapeutic target to block cardiovascular calcification. (nih.gov)
- Serum bone alkaline phosphatase is a marker of bone formation and urinary N-telopeptides are markers of bone resorption. (cdc.gov)
- There were two methods used to measure Bone Alkaline Phosphatase in NHANES 2001-2002. (cdc.gov)
- For NHANES 2001, the HybritechTandem-MP Ostase ImmunoEnzymetric assay was used for quantitative measurement of Bone Alkaline Phosphatase (BAP), an indicator of osteoblastic activity, in human serum. (cdc.gov)
- Psilocybin is dephosphorylated to Psilocin and inorganic phosphate by purified calf intestinal alkaline phosphatase. (erowid.org)
- Recombinant SEAP (secreted embryonic alkaline phosphatase) protein is a truncated form of human placental alkaline phosphatase that comprises 520 amino acids. (invivogen.com)
- 10 μg lyophilized recombinant human secreted embryonic alkaline phosphatase (SEAP). (invivogen.com)
- The overall goal of this project was to use the tools of physical organic chemistry to probe the mechanistic features of promiscuous enzymes in the alkaline phosphatase superfamily, a structurally related family of enzymes that appears to be particularly prone to promiscuous behaviour. (europa.eu)
- Designed for use with alkaline phosphatase-labeled conjugates. (seracare.com)
- The aim of this study was to determine the influence of age on intestinal brush-border disaccharidases (lactase, sucrase, and maltase), and alkaline phosphatase activity in CD26 deficient mice. (srce.hr)
- Alkaline phosphatase (ALP) serves as an important biomarker for the diagnosis of various diseases including liver malfunction, bone diseases, breast and prostate cancers, and diabetes. (amrita.edu)
- This paper discusses the fabrication and testing of a paper-based Point-of-care testing (POCT) device for the colorimetric detection of alkaline phosphatase (ALP). (amrita.edu)
- Rotator Cuff Tenocytes Differentiate into Hypertrophic Chondrocyte-Like Cells to Produce Calcium Deposits in an Alkaline Phosphatase-Dependent Manner. (iasp-pain.org)
- Calcifications were amorphous areas surrounded by a fibrocartilaginous metaplasia containing hypertrophic chondrocyte-like cells that expressed tissue non-specific alkaline phosphatase (TNAP) and ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1), which are two key enzymes of the mineralization process. (iasp-pain.org)
- The purpose of this study was to investigate the activities of the total acid phosphatase (TAP), tartrate-resistant acid phosphatase (TRAP), low molecular weight protein tyrosine phosphatase (LMW-PTP) and alkaline phosphatase (ALP) enzymes, as well as the possible correlation in the serum and in unstimulated whole saliva of children. (bvsalud.org)
- We retrospectively examined the correlation of serum alkaline phosphatase (ALP) levels with several clinicopathological characteristics of 207 cases of oesophageal carcinoma. (who.int)
- The details of the Alkaline phosphatase (ALP) com- oesophageal tumours had transtho- clinicopathological findings are sum- prises a group of enzymes that catalyse racic oesophagectomy (Ivor Lewis or marized in Table 1. (who.int)
- Because aminotransferases and alkaline phosphatase are included in commonly done laboratory test panels, abnormalities are often detected in patients without symptoms or signs of liver disease. (msdmanuals.com)
Acid Phosphatase6
- Lysophosphatidic acid phosphatase type 6 (ACP6) is an LPA-specific acid phosphatase that hydrolyzes LPA to monoacylglycerol (MAG) and phosphate. (rcsb.org)
- The following product was used in this experiment: Tartrate Resistant Acid Phosphatase Monoclonal Antibody (5C5E7) from Thermo Fisher Scientific, catalog # MA5-38435, RRID AB_2898349. (thermofisher.com)
- Tartrate-resistant acid phosphatase (TRAP) is a basic iron-binding protein with high activity towards phosphoproteins, ATP and 4-nitrophenyl phosphatase. (thermofisher.com)
- The reference value for acid phosphatase is 2 ng/mL or less. (medscape.com)
- Prostatic acid phosphatase (PAP), which is produced in the prostate, was the first major serum marker for prostate cancer, and was used widely for screening, staging, and post-treatment monitoring. (medscape.com)
- Semen acid- phosphatase activity was increased in the 45mg/kg group. (cdc.gov)
Dual-Specificity Phosphatase7
- DUSP9, a Dual-Specificity Phosphatase with a Key Role in Cell Biology and Human Diseases. (nih.gov)
- Expression of Dual-Specificity Phosphatase 9 in Placenta and Its Relationship with Gestational Diabetes Mellitus. (nih.gov)
- Find Dual Specificity Phosphatase 3 Antibodies for a variety of species such as anti-Human Dual Specificity Phosphatase 3, anti-Mouse Dual Specificity Phosphatase 3, anti-Rat Dual Specificity Phosphatase 3. (antibodies-online.com)
- Find Dual Specificity Phosphatase 3 Antibodies validated for a specific application such as WB, ELISA, IHC, IF (cc). (antibodies-online.com)
- Find Dual Specificity Phosphatase 3 Antibodies with a specific Host. (antibodies-online.com)
- Find available monoclonal or polyclonal Dual Specificity Phosphatase 3 Antibodies. (antibodies-online.com)
- Find Dual Specificity Phosphatase 3 Antibodies with a specific conjugate such as Biotin, FITC, HRP. (antibodies-online.com)
Striatal-enriched protein tyrosine ph4
- Here, we report that high levels of the striatal-enriched protein tyrosine phosphatase (STEP), a key regulator of ERK/MAPK signaling, are found in vulnerable somatostatin-immunoreactive hilar interneurons. (jneurosci.org)
- Our data reveal that the vulnerable cells express high levels of striatal-enriched protein tyrosine phosphatase (STEP), which is a key regulator of the extracellular signal-regulated kinase (ERK) pathway. (jneurosci.org)
- La protéine Striatal-Enriched Protein Tyrosine Phosphatase (STEP) joue un rôle important dans la régulation de la force synaptique, notamment par sa capacité à s'opposer au renforcement synaptique et à encourager la dépression à long terme. (umontreal.ca)
- The Striatal-Enriched Protein Tyrosine Phosphatase (STEP) plays an important role in the regulation of synaptic strength, namely through its ability to oppose synaptic strengthening and encourage long term depression. (umontreal.ca)
Enzymes6
- Phosphatase enzymes are essential to many biological functions, because phosphorylation (e.g. by protein kinases) and dephosphorylation (by phosphatases) serve diverse roles in cellular regulation and signaling. (wikipedia.org)
- Phosphatase enzymes are not to be confused with phosphorylase enzymes, which catalyze the transfer of a phosphate group from hydrogen phosphate to an acceptor. (wikipedia.org)
- In contrast to kinases, phosphatase enzymes recognize and catalyze a wider array of substrates and reactions. (wikipedia.org)
- With the Human Accell Phosphatases siRNA library, researchers receive siRNAs targeting enzymes with known or predicted phosphatase activity. (horizondiscovery.com)
- Cells depend on phosphatases to maintain tight control over the activity of several metabolic enzymes and signal transducing factors. (horizondiscovery.com)
- Acid phosphatases are enzymes that are capable of hydrolyzing phosphate esters in an acidic environment. (medscape.com)
Substrate9
- Because a phosphatase enzyme catalyzes the hydrolysis of its substrate, it is a subcategory of hydrolases. (wikipedia.org)
- Phosphatases catalyze the hydrolysis of a phosphomonoester, removing a phosphate moiety from the substrate. (wikipedia.org)
- Identifying the "phosphatase code," that is, the mechanisms and rules that govern substrate recognition for phosphatases, is still a work in progress, but the first comparative analysis of all the protein phosphatases encoded across nine eukaryotic 'phosphatome' genomes is now available. (wikipedia.org)
- Many phosphatases have yet to be discovered, and for numerous known phosphatases, a substrate has yet to be identified. (wikipedia.org)
- A protein phosphatase is an enzyme that dephosphorylates an amino acid residue of its protein substrate. (wikipedia.org)
- Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. (nih.gov)
- A shallow active site pocket in VHR allows for the hydrolysis of phosphorylated serine, threonine, or tyrosine protein residues, whereas the deeper active site of protein tyrosine phosphatases (PTPs) restricts substrate specificity to only phosphotyrosine. (rcsb.org)
- The vast hydrophobic substrate-binding pocket, which is located between those two domains, is suitable for accommodating LPA, and its shape is different from that of other histidine acid phosphatases, a fact that is consistent with the observed difference in substrate preferences. (rcsb.org)
- Pierce™ Phosphatase Substrate Kit, 1 kit - 1 kit is backordered and will ship as soon as it is back in stock. (dnamethsoc.com)
Kinases and phosphatases5
- Together, kinases and phosphatases direct a form of post-translational modification that is essential to the cell's regulatory network. (wikipedia.org)
- The tandem work of kinases and phosphatases constitute a significant element of the cell's regulatory network. (wikipedia.org)
- To achieve high biological specificity, protein kinases and phosphatases often recognize their targets through interactions that occur outside of the active site. (nih.gov)
- Although the role of modular protein-protein interaction domains in kinase and phosphatase signaling has been well characterized, it is becoming clear that many kinases and phosphatases utilize docking interactions - recognition of a short peptide motif in target partners by a groove on the catalytic domain that is separate from the active site. (nih.gov)
- Their spatial and temporal availability is tightly regulated by a set of specific lipid kinases and phosphatases . (bvsalud.org)
Phosphate8
- In biochemistry, a phosphatase is an enzyme that uses water to cleave a phosphoric acid monoester into a phosphate ion and an alcohol. (wikipedia.org)
- Whereas phosphatases remove phosphate groups from molecules, kinases catalyze the transfer of phosphate groups to molecules from ATP. (wikipedia.org)
- Phosphatases should not be confused with phosphorylases, which add phosphate groups. (wikipedia.org)
- Whereas protein kinases act as signaling molecules by phosphorylating proteins, phosphatases remove the phosphate group, which is essential if the system of intracellular signaling is to be able to reset for future use. (wikipedia.org)
- Lipid phosphate phosphatase-2 promotes tumor growth through increased c-Myc expression. (harvard.edu)
- Endothelial lipid phosphate phosphatase-3 deficiency that disrupts the endothelial barrier function is a modifier of cardiovascular development. (harvard.edu)
- Protein phosphatases remove phosphate groups covalently attached to serine, threonine, and tyrosine residues. (rndsystems.com)
- A subclass of receptor-like protein tryosine phosphatases that contain a single cytosolic protein tyrosine phosphate domain and multiple extracellular fibronectin III-like domains. (online-medical-dictionary.org)
Tyrosine Phosphatase3
- Protein tyrosine phosphatase nonreceptor type 2 (PTPN2) recently emerged as a promising cancer immunotherapy target. (jci.org)
- Here, a recently introduced serial sample-support chip system has been used to obtain the first SSX structure of a human phosphatase, specifically protein tyrosine phosphatase 1B (PTP1B) in the unliganded (apo) state. (iucr.org)
- protein tyrosine phosphatase, non-receptor. (wikigenes.org)
Phospholipid Phosphatase1
- Phospholipid Phosphatase 4 promotes proliferation and tumorigenesis, and activates Ca(2+)-permeable Cationic Channel in lung carcinoma cells. (nih.gov)
Phosphatidate phosphatase5
- and phosphatidate phosphatase activity. (nih.gov)
- Cloning and characterization of DPPL1 and DPPL2, representatives of a novel type of mammalian phosphatidate phosphatase. (nih.gov)
- Phosphatidate Phosphatase" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
- This graph shows the total number of publications written about "Phosphatidate Phosphatase" by people in Harvard Catalyst Profiles by year, and whether "Phosphatidate Phosphatase" was a major or minor topic of these publication. (harvard.edu)
- Below are the most recent publications written about "Phosphatidate Phosphatase" by people in Profiles. (harvard.edu)
PTPs1
- Among these negative regulators, protein tyrosine phosphatases (PTPs) are likely to play a pivotal role in IR signaling. (bioone.org)
Inhibition1
- By counteracting the activities of kinases, phosphatases play an important role in the control of a wide variety of cellular functions including cell cycle checkpoints, responsiveness to growth factors, contact inhibition, and cellular motility. (rndsystems.com)
Serine1
- The cocktail contains inhibitors against the major classes of proteases and phosphatases, targeting aminopeptidases, cysteine and serine proteases and serine/threonine and protein tyrosine phosphatases. (thermofisher.com)
Signal Transduction1
- Dual specificity protein phosphatases (DSPs) regulate mitogenic signal transduction and control the cell cycle. (rcsb.org)
Reagents1
- An arrayed collection of siRNA reagents for RNAi screening, targeting human phosphatases. (horizondiscovery.com)
Colorimetric1
- Quantitative (colorimetric) determination of the Psilocin formed showed that about 5 x 10-4 micromole was liberated per unit phosphatase per hour. (erowid.org)
Phosphorylation2
- Protein phosphorylation is a reversible and dynamic post-translational modification that is governed by the opposing activities of protein phosphatases and kinases. (rndsystems.com)
- Here, we show that the phosphoprotein phosphatase SppA has strong effects on polar growth and cell shape and that it reverses the AfsK-mediated phosphorylation of DivIVA. (lu.se)
Negative regulator1
- Using Ptpn22 −/− mice we demonstrate that the phosphatase PTPN22 is a highly selective, negative regulator of cDC2 homeostasis, preventing excessive population expansion from as early as 3 weeks of age. (frontiersin.org)
Proteins2
- Halt Protease and Phosphatase Inhibitor Cocktail is specifically optimized to protect proteins from degradation during extraction and purification. (thermofisher.com)
- This study adds a PPP-family protein phosphatase to the proteins involved in the control of polar growth and cell shape determination in S. coelicolor. (lu.se)
Regulate1
- Docking interactions can also allosterically regulate phosphatases and thus influence their catalytic activity. (wikipedia.org)
Mutations2
- Mutations in INPP5K, Encoding a Phosphoinositide 5-Phosphatase, Cause Congenital Muscular Dystrophy with Cataracts and Mild Cognitive Impairment. (bvsalud.org)
- In individuals exhibiting congenital muscular dystrophy , early-onset cataracts , and mild intellectual disability but normal cranial magnetic resonance imaging , we identified bi-allelic mutations in INPP5K, encoding inositol polyphosphate -5- phosphatase K. Mutations impaired phosphatase activity toward the phosphoinositide phosphatidylinositol (4,5)-bisphosphate or altered the subcellular localization of INPP5K. (bvsalud.org)
Human2
- To some extent, this disparity results from incomplete knowledge of the human phosphatome, that is, the complete set of phosphatases expressed in a cell, tissue, or organism. (wikipedia.org)
- Here, the crystal structure of a human DSP, vaccinia H1-related phosphatase (or VHR), was determined at 2.1 angstrom resolution. (rcsb.org)
PP2A2
- Two notable protein phosphatases are PP2A and PP2B. (wikipedia.org)
- Unlike Okadaic Acid, which only acts on protein phosphatase PP2A, Calyculin A inhibits both PP1 and PP2A. (abcam.com)
Activity3
- Disturbances in phosphatase activity are implicated in a wide variety of disease states such as colon cancer, obesity, and immunodeficiencies. (rndsystems.com)
- Their activity is regulated by the MAPK-phosphatase 1 (DUSP1), a key component of the anti-inflammatory response. (datadryad.org)
- These data link congenital muscular dystrophies to defective phosphoinositide 5- phosphatase activity that is becoming increasingly recognized for its role in mediating pivotal cellular mechanisms contributing to disease . (bvsalud.org)
Cells1
- In this week's Journal of Neuroscience, Jun Tan, University of South Florida, Tampa, and colleagues propose that the tendency of microglia to lean one way or the other depends on CD45, a transmembrane phosphatase found on microglia and some other immune cells. (alzforum.org)
Affinity1
- these motifs bind with low affinity to docking sites on the phosphatase, which are not contained within its active site. (wikipedia.org)
Residues1
- These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. (nih.gov)
Substrates1
- Phosphatases are able to dephosphorylate seemingly different sites on their substrates with great specificity. (wikipedia.org)
Type1
- Aucune corrélation statistiquement significative n'a été trouvée entre le taux de phosphatase alcaline sérique et le sexe, l'âge, le type histologique de la tumeur, sa localisation et sa taille, sa profondeur de pénétration, la présence de métastases à distance, le degré de différenciation, et la présence d'une atteinte des ganglions et de cancers multiples simultanés. (who.int)
Cellular1
- Due to their prevalence in cellular regulation, phosphatases are an area of interest for pharmaceutical research. (wikipedia.org)
Drugs1
- A number of successful drugs have been developed that target protein phosphatases. (rndsystems.com)
Cell1
- Thermo Scientific Halt Protease and Phosphatase Inhibitor Cocktail (100X) provides the convenience of a single solution with full protein sample protection for cell and tissue lysates. (thermofisher.com)