Phenotype specific receptors laminin. Medical search. Frequent questions

Polyclonal and monoclonal antibodies raised against chicken gizzard 5′-nucleotidase were tested in adhesion assays of embryonic chicken fibroblasts (CEF) for their ability to interfere with the adhesion process of these cells on either laminin or fibronectin substrata. (morulaivf.com)
The initial attachment process of CEF on fibronectin and laminin substrata was not influenced by preincubating these cells with antibodies against chicken gizzard 5′-nucleotidase. (morulaivf.com)
Cell motility measurements by computer-assisted time-lapse videomicroscopy of the alpha 7-transfected cells revealed an 8-10-fold increase in motility on laminin-1 and its E8 fragment, but not on fibronectin. (uea.ac.uk)
MMP7, also known as Matrilysin, is a definite relative with proteolytic activity against an array of biomolecules including proteoglycans, laminin, fibronectin, casein and moreover cellar membrane collagen type IV [17,18]. (scienceexhibitions.org)
During liver fibrosis, the composition of the ECM changes from one dominated by collagen IV and laminin [ 4 ], to one of fibrillar collagen, predominantly collagen I and III, as well as fibronectin [ 5 ]. (biomedcentral.com)
These chondrocyte/ECM interactions are regulated by a large family of transmembrane glycoproteins whereby beta 1 integrins are the most widely expressed and interact with several matrix proteins such as collagen, fibronectin, vitronectin and laminin [ 4 ]. (biomedcentral.com)

Moreover, integrins, laminins, NF-κB and other regulative molecules were found as crucial proteins. (nature.com)
The flow-dependent adhesion of platelets to those and other extracellular matrix components is stabilized by a panel of integrins, including integrin α 2 β 1 , α IIb β 3 , and α 6 β 1 (adhesive platelet population), while the initial activation of platelets is achieved by signaling via the collagen/laminin receptor, glycoprotein VI (GPVI). (frontiersin.org)
Collagen receptors include discoidin domain receptors, INTEGRINS, and glycoprotein VI. (lookformedical.com)
This could be considered to be part of a feedback loop as changes in ECM composition results in changes in HSC cell surface receptors, particularly integrins indicating that the ECM should not be regarded simply as an inert physical scaffold but a dynamic structure that is able to influence the phenotype of HSCs [ 2 , 8 , 9 ]. (biomedcentral.com)
1999). However, branching morphogenesis is not affected in laminin α5, γ2, or α3 knockouts even though integrins α3 and α6 are the primary adhesive receptors for α3 and α5 laminins. (biolamina.com)
The extracellular matrix (ECM), the noncellular component of the microenvironment, influences cell growth, survival, migration and tissue-specific differentiation through a repertoire of receptors including integrins, syndecans and discoidin receptors. (ucsf.edu)

The process of thrombus formation is considered to be initiated by von Willebrand factor (VWF) binding to exposed collagen or laminin in the damaged vessel wall, followed by shear-dependent platelet binding to VWF through the glycoprotein (GP)Ib-V-IX complex ( 1 , 3 , 4 ). (frontiersin.org)
Intraepidermal Type VII Collagen by Immunofluorescence Mapping: A Specific Finding for Bullous Dermolysis of the Newborn. (stanford.edu)
Collagen receptors are cell surface receptors that modulate signal transduction between cells and the EXTRACELLULAR MATRIX. (lookformedical.com)
Immunological investigations consisted of direct immunofluorescence (IF), indirect IF with intact and salt-split canine gingival, and salt-split normal or laminin-332-deficient human skin, immunoblotting with purified human laminin-332 and immunoblotting with recombinant NC1 domain of human collagen VII. (morulaivf.com)
HSCs express numerous cell surface receptors including both discoidin domain receptors (DDRs)-which are receptors for collagen I and many of the integrin subunits, which are a major family of cell surface receptors for extracellular molecules and play a critical role in many biological functions [ 7 , 10 ]. (biomedcentral.com)
Accordingly, to clarify the molecular basis of tissue-specific differentiation and disease, a variety of 3D systems exploiting natural ECMs have been developed, such as reconstituted basement membrane (rBM) and purified collagen hydrogels. (ucsf.edu)
Mammary glands from mice that underwent abrupt involution exhibited denser stroma, altered collagen composition, higher inflammation and proliferation, increased estrogen receptor α and progesterone receptor expression compared to those that underwent gradual involution. (biomedcentral.com)

Laminin, an extracellular matrix protein, is a major component of the basement membrane. (wikipedia.org)
KEGG pathways enrichment and PPI network indicate four major pathways, including extracellular matrix -receptor interaction, focal adhesion, phosphatidylinositol 3-kinase (PI3K)-Protein kinase B (Akt), and Ras signaling pathways were involved in the degeneration of cartilage. (nature.com)
Consistent with this hypothesis, developing granule cells from the rostral region of Gpr56 −/− cerebella at birth exhibit a specific defect in cell adhesion to extracellular matrix (ECM) molecules of the pial basement membrane (BM) but no defects in migration, proliferation, or process outgrowth. (jneurosci.org)
Dystroglycan is a core and essential component of the dystrophin-glycoprotein complex of muscle and brain, making vital links between the cytoskeleton and laminin in the extracellular matrix. (enmc.org)
This allows dystroglycan to function as the predominant extracellular matrix receptor in skeletal muscle and brain. (enmc.org)
Matriglycan is indeed a scaffold for multiple laminin-G (LG) domain-containing extracellular matrix proteins (e.g. laminin, agrin, and perlecan) and it has the remarkable capacity to be tuned during skeletal muscle development and regeneration. (enmc.org)
MMPs certainly are a category of endogenous calcium mineral- and zinc-dependent proteolytic enzymes that can handle degrading many extracellular matrix (ECM) elements, aswell as regulating various other enzymes, chemokines as well as cell receptors. (scienceexhibitions.org)
Our results demonstrate that cytoglobin expression is correlated with a more quiescent phenotype of stellate cells in culture and that cytoglobin is regulated by the extracellular matrix through integrin signalling dependent on activation of focal adhesion kinase. (biomedcentral.com)
The link between oncogenic RAS expression and the acquisition of the invasive phenotype has been attributed to alterations in cellular activities that control degradation of the extracellular matrix. (oncotarget.com)
Clone 13 however not Armstrong can displace extracellular matrix proteins such as for example laminin from αDG which talk about the same binding site as the trojan thus facilitating virus-receptor connections (18). (biospraysehatalami.com)

One of the major laminin receptors of myoblasts is the alpha 7 beta 1 integrin, which was first described in human MeWo melanoma cells and Rugli glioblastoma cells. (uea.ac.uk)

Previously we have shown that the locomotion of murine skeletal myoblasts is specifically and significantly enhanced on laminin but not on other matrix proteins. (uea.ac.uk)
Laminin is key proteins in the basement membranes of the airway and alveolar epithelium, endothelium, bronchial and vascular smooth muscle cells, nerve cells, and visceral pleura. (biolamina.com)

Laminin-332 polypeptides are targeted by auto-antibodies in human patients with mucous membrane (cicatricial) pemphigoid or, more rarely, subepidermal vesicular diseases that resemble epidermolysis bullosa acquisita (EBA) or bullous pemphigoid (BP). (morulaivf.com)
The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. (thermofisher.com)

In light of the severe motor phenotypes and apparent cerebellar abnormalities seen in BFPP patients, we studied the cerebellum of Gpr56 knock-out mice. (jneurosci.org)
Laminin α2 and α4 null mice survive to adulthood and have no obvious lung abnormalities (Nguyen and Senior, 2006). (biolamina.com)
Specific protein function and abnormalities are discussed below with each LGMD. (medscape.com)

2000). Laminin 111, expressed by epithelial and mesenchymal cells in the mouse developing lung, plays a role in branching morphogenesis and in epithelial cell polarity (Schuger et al. (biolamina.com)

In vitro assays demonstrated a competition of laminin and this monoclonal antibody for the binding site on purified 5′-nucleotidase. (morulaivf.com)
Isoform Alpha-7X2B and isoform Alpha-7X1B promote myoblast migration on laminin 1 and laminin 2/4, but isoform Alpha-7X1B is less active on laminin 1 (In vitro). (cusabio.com)
This macrophage polarization can be simulated in vitro using lipopolysaccharide (LPS) and interferon (IFN)-γ stimulation for a proinflammatory subset termed classical activation and stimulation with interleukin (IL)-4 and IL-13 for an alternative polarization phenotype. (haematologica.org)
In the present study, our aim was to establish an in vitro cell model based on patient-specific human neurons to study the pathomechanism of sporadic AD. (biomedcentral.com)

These findings prove that alpha 7 integrin expression confers a gain of function-motile phenotype to immobile cells and may be responsible for transduction of the laminin-induced cell motility. (uea.ac.uk)
found that the most significant changes in laminin and integrin expression pattern were found during the development from the pseudo glandular stage to the canalicular stage (Virtanen et al. (biolamina.com)
HoxA9 represses the malignant phenotype of breast cancer cells in vivo as well as in 3D rBM and this reversion is coincident with BRCA1 induction and normalization of adhesion and integrin expression. (ucsf.edu)

METHODS Mast cells were visualised by immunohistochemical staining of the mast cell specific proteases chymase and tryptase. (bmj.com)
The proteases involved are of different origins and types: (i) present as precursor in plasma, (ii) secreted into the plasma by activated platelets or other blood cells, or (iii) intracellularly activated and cleaving cytosolic receptor domains. (frontiersin.org)
Although these platelet activation processes are relatively well-studied as a function of the platelet environment, only since recently it is becoming clear that a multitude of proteases present in plasma or produced by platelets themselves are important for the distinct properties of platelet populations, often by cleaving specific receptors. (frontiersin.org)
Here, we review current knowledge how proteases act on platelet receptors and the platelet membrane surface. (frontiersin.org)
Cancer cells that have acquired an invasive phenotype release proteases that attack and digest the protein component of the matrix that would restrict the movement of these malignant cells. (oncotarget.com)

Mutations in GPR56 , an orphan G-protein-coupled receptor (GPCR), cause bilateral frontoparietal polymicrogyria (BFPP), a disorder characterized by mental retardation, seizures, motor developmental delay, and ataxia. (jneurosci.org)
Although these gross morphological changes have been characterized, the specific histopathologies underlying BFPP and the developmental processes affected by GPR56 mutations have remained unknown. (jneurosci.org)
To research the contribution of the newly discovered mutation towards the Clone 13 phenotype we utilized reverse-genetics methods to generate recombinant LCM infections with each one of these specific mutations. (biospraysehatalami.com)
This list continues to expand, and, as of this writing, specific mutations listed on OMIM are known for 7 autosomal dominant LGMDs and 23 autosomal recessive LGMDs. (medscape.com)
Overlap exists with Congenital Muscular Dystrophy (CMD) as several gene mutations can cause both a LGMD and CMD phenotype. (medscape.com)
Some mutations can cause both a myofibrillar myopathy and a muscular dystrophy phenotype. (medscape.com)
These diseases are discussed here in part because mutations in 2 genes can present with either an LGMD or a myofibrillar myopathy phenotype. (medscape.com)

Polyclonal and monoclonal antibodies against chicken gizzard 5 '-nucleotidase inhibit the spreading process of chicken embryonic fibroblasts on laminin substratum. (morulaivf.com)
The novel ten-1(et5) allele is a hypomorph since its post-embryonic phenotype is weaker than the null alleles ten-1(ok641) and ten-1(tm651) . (biomedcentral.com)
All five laminin α chains are present during early embryonic lung development but normal adult lung tissue contains primarily laminin α3, α4, and α5 chains (Miner et al,1997). (biolamina.com)
Laminin α4 and α5 are also expressed in vascular endothelial basement membrane in late embryonic lungs. (biolamina.com)

This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). (wikipedia.org)

Laminins are crucial for lung development where different laminin isoforms exhibit specific, non-overlapping functions. (biolamina.com)
1997) whereas laminin isoforms 211 and 221 have a role in smooth muscle cell differentiation. (biolamina.com)
Specific laminin isoforms are present in different tissue microenvironments and are essential for cell survival, proliferation, and differentiation. (biolamina.com)
This suggests that while a combination of Piezo2 and other Na V isoforms is sufficient to elicit activity in response to transient stimuli, Na V 1.1 is required for transmission of receptor potentials generated during sustained muscle stretch. (elifesciences.org)

Perturbations in the ability of dystroglycan to interact with laminin have severe implications for human disease. (enmc.org)
Furthermore, detailed biochemical and cell biological analyses have finely mapped the biosynthetic pathways leading to the glycosylation of dystroglycan that enable it to bind to laminin. (enmc.org)
This wealth of information has led to a new understanding of how the defective interaction between of dystroglycan and laminin leads to disease. (enmc.org)
was regarded solely in charge of the exceptionally solid binding affinity of Clone 13 (L at GP1 260) to its cellular receptor α-dystroglycan which among cells from the immune system is normally preferentially portrayed on dendritic cells and therefore alters dendritic cell function resulting in viral persistence. (biospraysehatalami.com)

Laminin subunit alpha-2 is a protein that in humans is encoded by the LAMA2 gene. (wikipedia.org)

My group is exploring the molecular mechanisms whereby ECM receptors modulate cell fate and tumorigenesis. (ucsf.edu)

In concordance with this observation, augmented surface expression of the C-C chemokine receptor 2 (CCR2) of mDC suggest an increased migration activity to inflamed peripheral tissues, such as the skin. (uni-marburg.de)
Laminin α1 expression is restricted to early human lung morphogenesis, whereas the expression of laminin α5 in the human lung is continuous from early lung development through adult life (Pierce et al. (biolamina.com)
1996). Expression of laminin α5 begins in early lung development and persists into adulthood with a widespread expression. (biolamina.com)
In conclusion, we provide evidence that proinflammatory polarization of macrophages does not lead to enhanced procoagulatory function, whereas alternative polarization of macrophages leads to an increased expression of TF and increased production of TF bearing extracellular vesicles by these cells suggesting a procoagulatory phenotype of alternatively polarized macrophages. (haematologica.org)
4 Upon polarization, macrophages react to the respective stimulus with the expression of a distinct phenotype. (haematologica.org)
7 In contrast, alternatively activated macrophages are characterized by increased expression of IL-10 and of scavenger receptors. (haematologica.org)
Anatomical profiling of G protein-coupled receptor expression. (guidetopharmacology.org)

and (Desk I). Besides these genes specific peptides or their analogues such as for example glucagon like peptide-1/exendin-4 may also be involved with islet regeneration. (immune-source.com)

Description: The APB5 monoclonal antibody reacts with the mouse CD140b molecule, the beta chain of the platelet derived growth factor receptor (PDGF receptor). (thermofisher.com)

Laminin-332 (laminin-5) is a basement membrane heterotrimeric protein composed of alpha-3, beta-3 and gamma-2 laminin chains. (morulaivf.com)

However, liver cirrhosis and intestinal stricture formation are not common in mast cell disease, indicating that besides mast cells additional factors or specific stimulation of mast cells may be necessary to induce severe fibrosis. (bmj.com)
The signaling pathways involved in initiating the EMT seem to primarily involve the Smad-dependent pathway, whereby TGFbeta binding to specific high affinity cell surface receptors activates the receptor-Smad/Smad4 complex. (nih.gov)
In order to investigate and directly test the role of the alpha 7 integrin in cell migration on laminin, we expressed the murine alpha 7B splice variant in human 293 kidney cells and 530 melanoma cells which cannot migrate on laminin and are devoid of endogenous alpha 7. (uea.ac.uk)
PDGFRb is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. (thermofisher.com)
are laminin-like diffusible chemotactic protein involved with pancreatic morphogenesis and are likely involved in the legislation of duct-cell and foetal GSK1838705A islet cell migration. (immune-source.com)
In summary, this experimental study convincingly confirmed that the PV mouse model reproduces the principle mechanisms of HLA-dependent and human Dsg3-specific induction of CD4+ T and B cell responses observed in PV patients. (uni-marburg.de)
Acts as Schwann cell receptor for laminin-2. (cusabio.com)
This method uses either epithelial cell-specific markers which are generally expressed in all tumor cells, or tumor markers expressed by specific types of cancer ( 13 ). (iiarjournals.org)
Studies of laminin α5-deficient mice indicate that laminins 511 and 521 are essential for normal lobar septation in early lung development and normal alveolarization and distal epithelial cell differentiation and maturation in late lung development (Nguyet et al, 2005). (biolamina.com)
Laminin 332 is well associated with cutaneous epithelial cell functions, however, laminin γ2 null mice have only minimal changes in lung development, and it's suggested that laminin 332 in the lung have a role in response to injury or disease processes. (biolamina.com)
Numerous scientists have found our products and finally succeeded in their specific stem cell application. (biolamina.com)
The uPA converts cell surface-bound plasminogen to plasmin, a process that is regulated by the binding of plasminogen to specific receptors on the cell surface, however, the identity of the plasminogen receptors that function in this capacity is unclear. (oncotarget.com)
Notwithstanding, to achieve the effectiveness and integration of the grafted tissue to the receptor site, it is necessary a strong cell adhesion, so that it demands many molecules interaction to induce cell differentiation and the bone matrix mineralization formed onto the graft. (bvsalud.org)

Even within the disease spectrum relating to a single gene, there is considerable variation in phenotype depending on the actual mutation. (enmc.org)

Upon prolonged high intracellular rises in Ca 2+ , platelets develop a procoagulant phenotype that is characterized by the surface membrane exposure of phosphatidylserine and by the inactivation of integrin α IIb β 3 (procoagulant platelet population) ( 5 ). (frontiersin.org)
Spreading-arrested and rounded CEF do not develop prominent intracellular stress-fibers like control cells, instead they seem to concentrate their available actin in areas of presumptive initial contact with the laminin substratum. (morulaivf.com)

An important way of regulation is provided by proteolytic cleavage of several of these receptors, leading to either a gain or a loss of platelet function. (frontiersin.org)

In five dogs, IgG variably recognized the basement membrane of laminin-332-deficient human skin (three dogs negative, two dogs positive). (morulaivf.com)
These observations establish laminin-332 as a novel basement membrane antigen in dogs with autoimmune blistering diseases with variable clinical phenotypes. (morulaivf.com)
Laminins α1, α2, and α3 are co-localized to the airway epithelial basement membrane during early lung development. (biolamina.com)
Laminin α4 is seen in the pericellular basement membrane of smooth muscle cells lining the airway epithelium (Nguyet and Senior, 2006). (biolamina.com)
Laminin α5 also co-localizes with laminins α1-α3 in the BM of airway epithelium and it is the only laminin α chain found in the visceral pleural basement membrane (Nguyet and Senior, 2006). (biolamina.com)

The colocalisation with laminin indicates a mechanism of interaction between smooth muscle cells and mast cells that may be important in the role of mast cells in the process of fibrosis. (bmj.com)
Since laminin 111 has been shown to play a role in branching morphogenesis, it has been suggested that laminin 111 could be central for this integrin interaction (Nguyet and Senior, 2006). (biolamina.com)

The integrin α6 null mouse does not have a reported lung phenotype, however integrin α3/integrin α6 double knockout mice have several features reminiscent of the laminin α5 knockout (De Arcangelis et al. (biolamina.com)

Acts as a receptor of COMP and mediates its effect on vascular smooth muscle cells (VSMCs) maturation. (cusabio.com)

It also was connected with intense tumor phenotype. (scienceexhibitions.org)
Different prognosis noticed for patients from the same scientific stage emphasizes the actual fact that the scientific stage cannot effectively reflect the natural behavior from the tumor and brand-new biological elements (e.g. biomarkers) are obligatory to complement scientific variables for more specific decision-making [3]. (scienceexhibitions.org)
targeting of ITGA7 by RNAi or blocking mAbs impaired laminin-induced signaling, and it led to a significant delay in tumor engraftment plus a strong reduction in tumor size and invasion. (cusabio.com)
Here, we show that although this receptor is present on most vascular and blood cells, the key regulatory function in tumor and wound angiogenesis is performed by β 3 integrin on bone marrow-derived cells (BMDCs) recruited to sites of neovascularization. (rupress.org)

Myofiber-specific ablation of IRE1α or XBP1 in mice diminishes skeletal muscle regeneration that is accompanied with reduced number of satellite cells. (elifesciences.org)
Myofiber-specific ablation of IRE1α dampens Notch signaling and canonical NF-κB pathway in skeletal muscle of adult mice. (elifesciences.org)

The absence of either alpha7beta1 integrin or alpha6beta1 integrin impairs the ability of Schwann cells to spread and to bind laminin. (cusabio.com)

Phenotype of CNTNAP1: a study of patients demonstrating a specific severe congenital hypomyelinating neuropathy with survival beyond infancy. (nih.gov)

Required to promote contractile phenotype acquisition in differentiated airway smooth muscle (ASM) cells. (cusabio.com)

Lung branching morphogenesis likely involves integrin interactions with laminins. (biolamina.com)

All mast cells in the muscularis propria were colocalised with patches of laminin. (bmj.com)
In contrast, in the submucosa, laminin was exclusively found in the basal lamina of blood vessels where many adherent mast cells were seen. (bmj.com)
However, the subsequent spreading process of these cells was found to be inhibited for at least 2 h on a laminin substratum. (morulaivf.com)
Mock-transfected cells did not migrate significantly of alpha 7-transfected 293 cells through laminin-coated filters in a Boyden chamber assay was significantly enhanced in comparison to mock transfected cells. (uea.ac.uk)
The occurrence of PV being associated with the presence of specific HLA class II alleles thereby reflects the high relevance of autoreactive CD4+ T cells in disease pathogenesis. (uni-marburg.de)
Compact disc4+ helper T cells support virus specific Compact disc8+ T cells in clearance of consistent infection and offer help B cells to create antibodies. (biospraysehatalami.com)

However, although the primary defect in many LGMDs is known, the precise mechanism leading to the dystrophic phenotype has not always been elucidated. (medscape.com)

Alternative polarization with interleukin-4 and IL-13 led to a macrophage phenotype characterized by increased tissue factor (TF) production and release and by an increase in extracellular vesicle production. (haematologica.org)

Phase 2 Trial of a Neurokinin-1 Receptor Antagonist for the Treatment of Chronic Itch in Epidermolysis Bullosa Patients: A Randomized Clinical Trial. (stanford.edu)
From Clinical Phenotype to Genotypic Modelling: Incidence and Prevalence of Recessive Dystrophic Epidermolysis Bullosa (RDEB). (stanford.edu)
The names 'acquired junctional epidermolysis bullosa', 'anti-laminin-332 mucous membrane pemphigoid (MMP)' and 'mixed auto-immune subepidermal blistering dermatosis' are proposed for dogs with clinical signs reminiscent of EBA, MMP or BP respectively. (morulaivf.com)

The activities of adhesion and signaling receptors in platelets are controlled by several mechanisms. (frontiersin.org)

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Fibronectin6

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