• cDNA microarray data (7). (lu.se)
  • ABSTRACT: We used a cDNA microarray to profile gene expression in the intertidal mussel Mytilus californianus across major portions of its biogeographic range. (int-res.com)
  • First-strand cDNA prepared from 5 biological replicates from each site were competitively hybridized to a 2496 feature cDNA microarray. (int-res.com)
  • Our goal is to begin to explore these interactions using rodent models of human disease and cDNA microarray assays to reveal patterns of gene expression. (jcvi.org)
  • We performed genome-wide assessment for structural abnormalities in 427 unrelated ASD cases via single-nucleotide polymorphism microarrays and karyotyping. (nih.gov)
  • Analysis of genome-wide microarrays reveals 2.8% of transcripts whose expression was regulated in at least one of the four experimental conditions: 47.3% of which were repressed by CRE1, whereas 29.0% were actually induced by CRE1, and 17.2% only affected by the growth rate but CRE1 independent. (biomedcentral.com)
  • The presence of subtle DNA copy number changes was investigated by array-CGH in 50 patients with learning disability and dysmorphism, employing a DNA microarray constructed from large insert clones spaced at approximately 1 Mb intervals across the genome. (bmj.com)
  • Brown PO, Botstein D: Exploring the new world of the genome with DNA microarrays. (karger.com)
  • Next, mRNA samples were subjected to whole genome microarray and rtPCR analyses for toxicogenomic evaluation. (cdc.gov)
  • Background: Genome wide microarray studies have the potential to unveil novel disease entities. (sciweavers.org)
  • Topics covered include various high-throughput genomics technologies (genome sequencing, DNA microarrays, proteomics), quantitative modeling of transcriptional and post-transcriptional regulatory networks, synthetic biology, and the world of RNA structure, dynamics, and function. (columbia.edu)
  • Expanding the genotype-phenotype correlation in subtelomeric 19p13.3 microdeletions using high resolution clinical chromosomal microarray analysis. (bvsalud.org)
  • Diagnosis is made by confirming a deletion in the WHS critical region (WHSCR) using conventional chromosome analysis, fluorescence in situ hybridization (FISH), or chromosomal microarray. (medscape.com)
  • However, few studies have comprehensively evaluated its performance against current standard-of-care diagnostic tests: karyotype, chromosomal microarray (CMA), and exome sequencing (ES). (cdc.gov)
  • Just as DNA microarrays and proteomic technologies have made it possible to assay the expression level of thousands of genes or proteins all a once, phenotype microarrays (PMs) make it possible to quantitatively measure thousands of cellular phenotypes simultaneously. (wikipedia.org)
  • This workflow searches for genes which are found to be differentially expressed in a microarray study using Arabidopsis thaliana. (myexperiment.org)
  • Genomic microarray can detect ROH, which may indicate an increased risk for autosomal recessive (AR) disease for genes contained within the ROH, and/or the risk of an imprinting disorder due to uniparental disomy (UPD), or molar pregnancy. (arupconsult.com)
  • DNA microarrays make it possible to analyze the mRNA expression of thousands of genes simultaneously. (karger.com)
  • Therefore, we have recently developed the tissue microarray (TMA) technology to efficiently test the clinical relevance of candidate genes. (karger.com)
  • DNA microarrays and TMAs provide a powerful approach to identify large numbers of new candidate genes, and rapidly validate their clinical impact in large series of human tumors. (karger.com)
  • Microarray analysis reveals that inhibition of Notch signalling causes downregulation of stem cell-associated genes in parallel with decreased proliferation and increased numbers of ciliated cells and that organoids also respond to oestradiol and progesterone treatment in a physiological manner. (nature.com)
  • While we have a long history of associating genes and gene defects with a large array of disease phenotypes," said Dr. Quackenbush, "studies increasingly suggest that many disease phenotypes occur through the interactions of genes with their environments, including the genetic background in which the genes are expressed. (jcvi.org)
  • Gene Prospector provides an online gateway for searching for evidence about human genes in relation to diseases, other phenotypes, and risk factors, and provides links to published literature and other online data sources. (cdc.gov)
  • Proprietary and commercially available software is available that provides a solution for storage, retrieval, and analysis of high throughput phenotype data. (wikipedia.org)
  • Other software tools are PheMaDB, which provides a solution for storage, retrieval, and analysis of high throughput phenotype data, and the PMViewer software which focuses on graphical display but does not enable further statistical analysis. (wikipedia.org)
  • Microarray analysis is a method that uses microchips containing anchored arrays of short DNA elements (known as probes) for the large-scale interrogation of gene expression. (nature.com)
  • Phenotypic microarray analysis with 950 chemical substrates to assess nutrient utilization and stress resistance revealed phenotype differences between serovars. (usda.gov)
  • Cytogenomic SNP microarray testing is used to identify genomic imbalances (deletions and duplications) and may be used to further characterize abnormalities identified by chromosome analysis, including unbalanced translocations, recombinant chromosomes, markers, and ring chromosomes. (arupconsult.com)
  • Genomic microarray can detect chromosomal imbalances at a much higher level of resolution than standard chromosome analysis. (arupconsult.com)
  • Microarray-based comparative genomic hybridization is a method of molecular analysis that identifies chromosomal anomalies (or copy number variants) that correlate with clinical phenotypes. (nih.gov)
  • Microarray analysis revealed the previously reported mutation p.Y63X in two unrelated patients, one of them homozygous for the mutation. (molvis.org)
  • We can help researchers identify SNPs, indels, gene copy numbers, and genomic rearrangements from the various types of DNA-sequencing and microarray data and provide tailored downstream analysis of variants and mutations. (nottingham.ac.uk)
  • Locus refinement was undertaken with single nucleotide polymorphism (SNP) microarray analysis. (molvis.org)
  • The single nucleotide polymorphism microarray analysis excluded a shared founder haplotype between the families and refined the locus to 3.1 Mb. (molvis.org)
  • Our proposal builds on existing expertise at TIGR in the analysis of gene expression using cDNA microarrays, the mouse mutagenesis and phenotyping programs that have been developed by The Jackson Laboratories and their collaborators at Penn, Duke, and Boston Universities, and the efforts in rat genomics underway at the Medical College of Wisconsin," said Dr. Quackenbush. (jcvi.org)
  • Gene expression patterns in mussels from Strawberry Hill, Oregon, displayed a unique expression phenotype that was distinct from the other 3 mussel populations sampled. (int-res.com)
  • Structural variants were found in sufficiently high frequency influencing ASD to suggest that cytogenetic and microarray analyses be considered in routine clinical workup. (nih.gov)
  • Microarray technologies are already increasingly being used in urologic research, and will also have a strong impact on clinical urology. (karger.com)
  • Furthermore, integrating CD8 + T:Foxp3 ratios, which increased the complexity for immune phenotype status, revealed 6-7 clusters that enabled the separation of gastric cancer patients at the same clinical stage into different risk-group subsets. (biomedcentral.com)
  • Characterizing immune phenotypes in human gastric disease tissues via multiplexed immunohistochemistry may help guide PD-L1 clinical therapy. (biomedcentral.com)
  • The identification of chiasmal misrouting in family F1 and anterior segment abnormalities in family F2 suggested that the families have the same clinical phenotype. (molvis.org)
  • In this study, we extend the clinical phenotypes observed in each family and show that they have the same disorder, which we have termed f oveal h ypoplasia, o ptic n erve d ecussation defects and a nterior segment dysgenesis (FHONDA syndrome). (molvis.org)
  • It automatically discovers molecular heterogeneities in phenotypically defined disease entities and suggests alternative molecular sub-entities of clinical phenotypes. (sciweavers.org)
  • Numerous vendors from academic and private industry were promoting clinical sequencing services, microarray technologies, and health informatics software relevant to managing genomics data. (cdc.gov)
  • The third clinical category results from a very large deletion that exceeds 22-25 Mb, causing a severe phenotype that can hardly be defined as typical Wolf-Hirschhorn syndrome. (medscape.com)
  • The mRNA microarray datasets, GSE40057 and GSE1561, were downloaded and remodelled. (preprints.org)
  • The phenotype microarray approach is a technology for high-throughput phenotyping of cells. (wikipedia.org)
  • A phenotype microarray system enables one to monitor simultaneously the phenotypic reaction of cells to environmental challenges or exogenous compounds in a high-throughput manner. (wikipedia.org)
  • Topics covered will include high-throughput assay development, screening, chemical library creation, high-throughput chemistry, affinity purification of target proteins and target validation, protein microarrays, molecular evolution, protein engineering and synthetic biology. (columbia.edu)
  • It is the recommended first-tier test for patients undergoing prenatal diagnosis for the indication of a fetal structural abnormality detected by ultrasound (unless the structural abnormality is strongly suggestive of a specific aneuploidy, in which case, karyotype with or without fluorescence in situ hybridization [FISH] may be offered before genomic microarray). (arupconsult.com)
  • The phenotypic microarray data indicates that M. phaseolina can adapt to a wide range of osmotic and pH environments. (biomedcentral.com)
  • Using a phenotypic microarray (PM), we provided a full metabolic profile comparing C. sakazakii SP291 and the previously sequenced C. sakazakii ATCC BAA-894. (northumbria.ac.uk)
  • Another free and open source software developed to analyze Phenotype Microarray data is "DuctApe", a Unix command-line tool that also correlates genomic data. (wikipedia.org)
  • Our experience in working with RNA-sequencing or expression microarray data can enable researchers to pinpoint the molecular mechanisms that create associations between genotypes and phenotypes. (nottingham.ac.uk)
  • This four-credit course will present a quantitative description of the molecular networks that underlie the myriad phenotypes of living cells, from yeast to human. (columbia.edu)
  • However, a microdeletion of band 4p16.3, detected only by molecular probes, is usually associated with a milder phenotype without malformations. (medscape.com)
  • Another goal was to test the association between a positive microarray-based comparative genomic hybridization result and the severity of intellectual disability/developmental delay. (nih.gov)
  • Microarray-based comparative genomic hybridization identified structural chromosomal alterations responsible for the intellectual disability/developmental delay phenotype in 16% of our sample. (nih.gov)
  • We want to emphasize the need to conduct microarray-based comparative genomic hybridization on all individuals with intellectual disability/developmental delay, regardless of the severity, because the degree of intellectual disability/developmental delay does not predict the diagnostic yield of microarray-based comparative genomic hybridization. (nih.gov)
  • There is a need to further characterize the natural history of ZSD, the Gly470Ala variant, and expand upon possible genotype-phenotype correlations. (stanford.edu)
  • First, inexpensive genotyping and sequencing have made studies of genotype/phenotype correlations accessible to the scientific masses. (cdc.gov)
  • To screen and validate immunosuppressive factors in luminal- and basal-like breast cancer cell lines and tissue samples associated with malignant phenotypes. (preprints.org)
  • qRT-PCR and western blot were used to validate the expression of CD274 and IL8 in cell lines and immunohistochemical detected the MIF and VEGFA on tissue microarrays. (preprints.org)
  • By observing cell phenotypes within the disease tissue microenvironment, we were able to determine specific co-localized staining combinations and various measures of cell density. (biomedcentral.com)
  • Observing unique disease tissue microenvironments can improve our understanding of immune phenotypes and cell interactions within these microenvironments, providing the ability to predict safe responses to immunotherapies. (biomedcentral.com)
  • They were using a widely used microarray based platform specifically designed to identify zoonotic pathogens. (nottingham.ac.uk)
  • MT-ATP6 mitochondrial disease identified by newborn screening reveals a distinct biochemical phenotype. (stanford.edu)
  • All molecularly confirmed individuals (n=17) with either no symptoms (n=12), migraines (n=1), or a neurogenic muscle weakness, ataxia, and retinitis pigmentosa (NARP) phenotype (n=3) were found to have an A or U mitochondrial haplogroup, while one child with infantile-lethal Leigh syndrome had a B haplogroup. (stanford.edu)
  • however, having a "more coupled" (versus "less coupled") phenotype enables CR-induced improvements in muscle mitochondrial function. (medscape.com)
  • Three different categories of the Wolf-Hirschhorn syndrome phenotype are defined and generally correlate with the extent of the 4p deletion. (medscape.com)
  • Enrichment analyses performed and the online resources, GOBO and Kaplan-Meier Plotter, were employed to screen for immunosuppressive factors associated with breast cancer malignant phenotypes. (preprints.org)
  • Understanding immune phenotypes and human gastric disease in situ requires an approach that leverages multiplexed immunohistochemistry (mIHC) with multispectral imaging to facilitate precise image analyses. (biomedcentral.com)
  • Clinically, the minimal diagnostic criteria for Wolf-Hirschhorn syndrome (ie, ''core'' phenotype) consists of typical facial appearance, intellectual disability, growth delay, and seizures (or EEG anomalies). (medscape.com)
  • With microarrays, we discovered 277 unbalanced CNVs in 44% of ASD families not present in 500 controls (and re-examined in another 1152 controls). (nih.gov)
  • Effects of Deletion of Mutant Huntingtin in Steroidogenic Factor 1 Neurons on the Psychiatric and Metabolic Phenotype in the BACHD Mouse Model of Huntington Disease. (lu.se)
  • However, because individual immune-related factors can play several biological roles, the mechanistic relationship between immunosuppressive factors and breast cancer malignant phenotypes and the feasibility of their application as drug targets require further investigation. (preprints.org)
  • Classes to work with Phenotype Microarray data. (biopython.org)
  • PlateRecord object for storing Phenotype Microarray plates data. (biopython.org)
  • Bowtell DD: Options available - from start to finish - for obtaining expression data by microarray. (karger.com)
  • Microarrays were prepared in tumor biology. (lu.se)
  • An operational definition of asthma developed by experts logical mechanisms of different asthma phenotypes, including in the field (1) describes major attributes of the disorder. (cdc.gov)
  • Defective development of the embryonic radial ray (eg, aplasia, hypoplasia, fusion, other anomalous development) results in a wide spectrum of phenotypes, including triphalangeal or absent thumbs, foreshortened arms, and phocomelia. (medscape.com)
  • A written summary and an interpretation of the microarray findings are provided. (arupconsult.com)
  • The second and far more frequent category is identified by large deletions that average 5-18 Mb and cause the widely recognizable Wolf-Hirschhorn syndrome phenotype. (medscape.com)
  • New technologies such as microarrays are not only important for fundamental research, but will also be useful for diagnostic, prognostic or therapeutic purposes in individual patients. (karger.com)
  • Numerous asthma phenotypes have been described, including occupational asthma, and therapeutic strategies for asthma control are similar regardless of phenotype. (cdc.gov)
  • Directing hepatic differentiation of embryonic stem cells with protein microarray-based co-cultures. (ca.gov)
  • Printing columns of collagen (I) and fibronectin spots (300 microm diameter), followed by sequential seeding of the two cell types, allowed the positioning of clusters of mESCs adjacent to groups of hepatic cells within the same microarray. (ca.gov)
  • In conclusion, micropatterned co-cultures of mESCs and hepatic cells showed a significant promise in driving stem cell differentiation towards hepatic phenotype. (ca.gov)
  • A srvg23535 null mutant was constructed and characterized by using Phenotype MicroArray (PM) technology. (figshare.com)
  • using DNA microarray technology. (lu.se)
  • We have previously described two families with unique phenotypes involving foveal hypoplasia. (molvis.org)