Phenotype hepatocellular carcinoma cells micro rna that regulates. Medical search. Frequent questions

MicroRNAs (miRNAs) are small noncoding endogenous RNAs containing 19 to 25 nucleotides that promote posttranscriptional control in regulating the expression of target gene by binding to the 3′-UTR sequences of its mRNA [ 5 ]. (hindawi.com)
Recently, increasing evidences elucidated that miRNAs was involved in the cancer pathogenesis, including cell proliferation, metastasis, and apoptosis [ 6 ]. (hindawi.com)
microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. (cancerindex.org)
miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. (cancerindex.org)
Emerging evidence indicates that Long non-coding RNAs (LncRNAs) and microRNAs (miRNAs) play crucial roles in tumor progression, including hepatocellular carcinoma (HCC). (biomedcentral.com)
A series of studies indicate that lncRNAs serve as competing endogenous RNAs (ceRNA) by sponging miRNAs, and modulate the targets of miRNAs [ 15 , 16 ]. (biomedcentral.com)
MicroRNAs (miRNAs) are ~22-nt non-coding RNAs that negatively regulate gene expression at the post-transciptional level via direct binding with the 3′ untranslated regions (UTR) of target mRNAs ( 16 , 17 ). (spandidos-publications.com)
In the case of RNA molecules that bind target RNA segments, such as microRNAs (miRNAs), the altered base pairing can change binding specificities. (biomedcentral.com)
In recent years, emerging studies have highlighted the critical role of these pathways and their regulation by microRNAs (miRNAs) in cancer invasion and metastasis. (intechopen.com)
MiRNAs, short (18-24 nucleotides) non-coding RNAs, are derived from long transcripts pri-miRNAs and pre-miRNAs [ 26 - 30 ].By targeting 3' untranslated regions (3'UTRs) of cognate mRNAs, miRNAs post-transcriptionally regulate gene expression and induce translational repression [ 29 , 30 ]. (intechopen.com)
To date, 1527 human miRNAs have been identified (Sanger miRBase 18 http://www.miRbase.org/index.shtml), forming less than 1% of all human genes, potentially regulating more than 10% of all protein coding genes [ 1 ]. (intechopen.com)
MicroRNAs (miRNAs) are small non-coding RNA molecules that play a crucial role in the regulation of gene expression. (silexon.ai)
In our research, microRNAs (miRNAs) binding to the genome sequences of severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory-related coronavirus (MERS-CoV), and SARS-CoV-2 were identified by bioinformatic tools. (molcells.org)
MicroRNAs (miRNAs) are a class of small non-coding RNA, 20-22 nucleotides in length. (molcells.org)
The seed region in miRNAs, approximately six nucleotides long, recognizes and binds to the complementary binding site of the target mRNA 3'untranslated region (3'UTR) to regulate its expression. (molcells.org)
MicroRNAs (miRNAs) are master regulators involved in multiple physiological and pathological processes. (genengnews.com)
It's been suggested that miRNAs regulate up to 50% of all mRNAs in the human genome. (genengnews.com)
Both miRNAs and siRNAs need to be delivered into the target tissue or cell in order to activate the desired therapeutic effect," Muthiah Manoharan, Ph.D., senior vp, drug discovery, Alnylam Pharmaceuticals, explained. (genengnews.com)
MicroRNAs (miRNAs) play a critical role in regulating macrophage proliferation and differentiation and control the balance of M1 and M2 macrophage polarization, thereby controlling immune responses. (encyclopedia.pub)
The miRNAs that polarize macrophages towards a pro-inflammatory M1 phenotype ( Table 1 , Figure 1 ) are important in balancing the anti-inflammatory/regulatory M2 macrophages which promote Th2 immune responses. (encyclopedia.pub)
Let-7f miRNAs belong to a highly conserved let-7 (lethal-7) microRNA family consisting of 12 genes encoding for nine different miRNAs (let-7a to let-7i). (encyclopedia.pub)
MicroRNAs (miRNAs, miRs) are endogenously expressed small non-coding RNAs that generally regulate gene expression by binding to the 3ʹ untranslated region (3ʹ-UTR) of their targets, resulting in silencing [ 13 ]. (biomedcentral.com)
DEFB1 expression after transfection with two micro RNAs (miRNAs), hsa-miR-186-5p and hsa-miR-340-5p, provided evidence that DEFB1 expression could be modulated by these miRNAs and hsa-miR-186-5p had a higher binding efficiency with DEFB1. (cdc.gov)
Simple Summary: This study identified a set of 73 microRNAs (miRNAs) that can accurately detect lung cancer tumors from normal lung tissues. (cdc.gov)
This study identified a set of 73 microRNAs (miRNAs) that classified lung cancer tumors from normal lung tissues with an overall accuracy of 96.3% in the training patient cohort (n = 109) and 91.7% in unsupervised classification and 92.3% in supervised classification in the validation set (n = 375). (cdc.gov)
Experimentally confirmed target genes were identified for the 73 diagnostic miRNAs, from which proliferation genes were selected from CRISPR-Cas9/RNA interference (RNAi) screening assays. (cdc.gov)
MicroRNAs (miRNAs) são pequenos RNAs não-codantes, conservados ao longo da evolução, capazes de regular a expressão gênica através da degradação ou repressão da tradução de moléculas-alvo de RNA mensageiro. (bvsalud.org)
O presente estudo realiza uma revisão da literatura sobre miRNAs, enfocando aspectos relacionados à biogênese, mecanismos de ação e o papel potencial desses pequenos RNAs na carcinogênese oral. (bvsalud.org)
Como os miRNAs podem regular mais de um RNAm-alvo 33 , estima-se que estes pequenos RNAs controlem a expressão de aproximadamente 30% dos genes que codificam proteínas 23 . (bvsalud.org)
O presente estudo realiza uma revisão da literatura sobre miRNAs, enfocando aspectos relacionados à biogênese, mecanismos de ação e o papel potencial dessas pequenas moléculas de RNA na carcinogênese oral. (bvsalud.org)

High level expression of miR-17 family members induces cell proliferation, whereas deletion of the miR-17~92 cluster, in mice, is lethal and causes lung and lymphoid cell developmental defects. (wikipedia.org)
miR-455 suppressed cell proliferation in vitro and in vivo, and it inhibited the abilities of cell invasion and EMT in HCC. (hindawi.com)
Also, in gastric cancer, miR-455 inhibited human cell proliferation and invasion and promoted cell apoptosis [ 12 ]. (hindawi.com)
Further, high NEK2 expression promoted proliferation, colony formation, migration and invasion of HCC cell lines. (oncotarget.com)
CCK-8 assay was performed to examine cell proliferation. (biomedcentral.com)
Functional experiments demonstrated that knockdown of PTTG3P inhibited cell proliferation, migration and invasion, and promoted cell apoptosis, acting as an oncogene. (biomedcentral.com)
Extracellular ST6GAL1, present in cancer exosomes or the freely soluble recombinant sialyltransferase, compensates for insufficient intrinsic ST6GAL1 by boosting cancer cell proliferation and increasing invasiveness. (nature.com)
Our data indicate that extracellular ST6GAL1 from remote sources can compensate for cellular ST6GAL1-mediated aggressive tumor cell proliferation and invasive behavior and has great clinical potential for extracellular ST6GAL1 as these molecules are in the extracellular space should be easily accessible targets. (nature.com)
However, recently Houghton et al ( 7 ) showed increased cell proliferation after silencing of IRS1 gene expression in A549 cells suggesting tumor suppression potential. (spandidos-publications.com)
An aberrant miRNA expression could contribute to cancer development and progression [ 6 , 7 ] and could affect their target genes that are involved in many biological processes, such as cell differentiation, proliferation, apoptosis, metabolism, and development [ 8 ]. (hindawi.com)
Its downregulation is often observed in these cancers, contributing to uncontrolled cell proliferation and metastasis. (silexon.ai)
Then, the chemical count kit-8 cell viability assay, Annexin V-FITC apoptosis detection, EdU labeling proliferation detection, transwell and wound healing experiments were performed. (researchsquare.com)
Therefore, besides the regulation of immune cells, NFAT2 also exerts modulatory effect of proliferation, invasion, metastasis and malignancy in breast cancer, lung cancer, melanoma and leukemia [13-15]. (researchsquare.com)
In this study, we constructed NFAT2 overexpressed cells (HepG2/NFAT2) based on HepG2 and investigated the effect of NFAT2 overexpression on HepG2 cells' viability, apoptosis, proliferation, invasion and migration. (researchsquare.com)

The miR-17 microRNA precursor family are a group of related small non-coding RNA genes called microRNAs that regulate gene expression. (wikipedia.org)
With the exception of miR-93, these microRNAs are produced from several microRNA gene clusters, which apparently arose from a series of ancient evolutionary genetic duplication events, and also include members of the miR-19, and miR-25 families. (wikipedia.org)
The paralogous miRNA gene clusters that give rise to miR-17 family microRNAs (miR-17~92, miR-106a~363, and miR-106b~25) have been implicated in a wide variety of malignancies and are sometimes referred to as oncomirs. (wikipedia.org)
Pseudogene, a subclass of long non-coding RNAs (lncRNAs), are considered as genomic loci that resemble real gene, but lost some functionality because they are lack of protein-coding ability because of disabling mutation, lack of transcription, or their inability to encode RNA [ 3 ]. (biomedcentral.com)
Furthermore, bioinformatics approaches are employed to provide in silico evidences for the link between long noncoding RNAs and mitochondrial gene interaction in obesity. (informaticsstudies.org)
NcRNAs play a significant role in regulating gene expression in digestive system tumors. (ijbs.com)
This class of noncoding RNAs is small, single stranded, and 19-25 nucleotide long that act as negative regulators involved in posttranscriptional silencing of the gene expression [ 5 ]. (hindawi.com)
MicroRNA-34a gene (MIR-34A) that is located on chromosome 1p36 belongs to one of evolutionary-conserved miRNA families (MIR-34 family) that consists of three members: MIR-34A, MIR-34B, and MIR-34C [ 10 ]. (hindawi.com)
RNA sequences of a gene can have single nucleotide variants (SNVs) due to single nucleotide polymorphisms (SNPs) in the genome, or RNA editing events within the RNA. (rna-seqblog.com)
Differences in gene-specific methylation between cancer cells and their normal counterparts were described more than 30 years ago. (esmed.org)
Over the past 10 years we have been witness to an explosion of investigation into the epigenetic basis of cancer, and application of the powerful genome-wide DNA methylation profiling techniques to be reviewed have yielded critical insights into the organization of the cancer methylome with its broad regions of hypomethylation and foci of hypermethylation resulting in critical differences in gene expression and chromosomal stability compared to normal cells. (esmed.org)
These insights, in turn, have prompted novel, testable hypotheses, to be discussed, pertaining to fundamental aspects of cancer biology including the potential stem cell/progenitor cell origins of cancer and the plasticity of gene expression that may underlie tumor heterogeneity and tumor progression. (esmed.org)
Hypoxia was found to induce A498 cell invasion, migration, and the release of inflammatory cytokines, while repressing human solute carrier family 14 member 1 gene expression. (biomedcentral.com)
Overexpression of the solute carrier family 14 member 1 gene could abolish hypoxia-induced invasion, reduce the migration of A498 cells, inhibit the hypoxia-induced release of inflammatory cytokines, and arrest the cell cycle at the G1/S checkpoint. (biomedcentral.com)
The results elucidate a mechanism wherein the solute carrier family 14 member 1 gene participates in the occurrence and development of hypoxia-induced renal cell carcinoma in a mitochondria-dependent manner. (biomedcentral.com)
Transcription factors (TFs) drive gene expression programs that shape specific phenotypes [ 3 ], and are frequently dysregulated in cancer [ 4 ]. (aging-us.com)
Thus, MRs comprise typically a small number of TF-encoding genes (and their products) that control a disproportionate level of gene expression, giving rise to distinct molecular phenotypes associated with a particular disease. (aging-us.com)
Since the expression of genes defining discrete phenotypes is highly coordinated, application of reverse engineering algorithms to transcriptome datasets allows interpreting transcriptional networks by defining MRs and their associated regulons and gene circuits. (aging-us.com)
The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. (cancer-genetics.org)
For the current study, we performed transcriptome and small RNA sequencing to profile gene expression in SB-induced HCCs in an attempt to clarify the genetic element(s) contributing to tumorigenesis. (prolekarniky.cz)
Significant downregulation of beta-defensin1 gene (DEFB1) expression was observed when human bronchial epithelial cells (HBEpCs) were exposed to IAV. (cdc.gov)
MicroRNAs (miRNA) are small noncoding RNAs, preserved throughout the evolution, able to regulate gene expression through repression of translation or degradation of target molecules of messenger RNA. (bvsalud.org)

Mechanism of miR-455-3 in suppressing epithelial-mesenchymal transition and angiogenesis of non-small cell lung cancer cells. (nih.gov)
Microcrystalline silica particles induce inflammatory response via pyroptosis in primary human respiratory epithelial cells. (nih.gov)
Although the therapeutic potential of metformin for retinopathy disorders has recently been elucidated, possibly through AMPK activation, it remains unknown how metformin directly affects the MGO-induced stress response in retinal pigment epithelial cells. (bvsalud.org)
Metformin and A769662 via AMPK activation exert a strong protection against MGO-induced retinal pigment epithelial cell death and retinopathy. (bvsalud.org)
Aggressive breast cancer is a highly heterogeneous disease caused by a variety of distinct cell-intrinsic genetic alterations in mammary epithelial cells, leading to vastly heterogenic disease manifestation in individual patients and predominantly affecting patient prognosis and treatment options [ 14 ]. (nature.com)
Cells undergoing EMT lose their epithelial characteristics and phenotype, acquire mesenchymal properties, and thus gain enhanced motility ( 12 ). (spandidos-publications.com)
The epithelial-mesenchymal transition (EMT) is an example of cellular plasticity, where an epithelial cell acquires a mesenchymal-like phenotype that increases its migratory and invasive properties. (mdpi.com)
Initial works revealed that activation of the EMT program in epithelial cells induces the acquisition of stem cell properties, which in the context of cancer may contribute to the appearance of tumor initiating cells (TIC). (mdpi.com)
EMT is an embryologically conserved genetic program by which epithelial cells down regulate intercellular tight junctions, loose polarity, express mesenchymal markers, and manifest a migratory phenotype [ 1 ]. (intechopen.com)
Renal cell carcinoma (RCC) originates from either the cancerous renal cortex or renal tubular epithelial cells, and is the second most lethal urological malignancy, accounting for 90-95% of kidney neoplasms. (biomedcentral.com)
The primary barriers for invading respiratory pathogens are the respiratory tract epithelial cells and antimicrobial proteins generated by these cells. (cdc.gov)

Thus, the pivotal roles of miR-455 in cell viability and metastasis in HCC still needed to be explored. (hindawi.com)
The crosstalk between pro-tumorigenic immune cells, stromal cells, and cytokines helps to establish the pre-metastatic niche for disseminated circulatory tumor cells and facilitates metastasis. (biomedcentral.com)
During metastasis, tumor-derived exosomes help in the requirement and arrangement of immunosuppressive immune cells for favorable premetastatic niche formation and growth of metastases. (biomedcentral.com)
Lymph node metastasis is responsible for the high morbidity of head and neck squamous cell carcinoma (HNSCC). (spandidos-publications.com)
In this study, we found a negative correlation of IRS-1 expression with tumor metastasis both in human tissue samples and in cell lines. (spandidos-publications.com)
Hypoxia is considered a critical contributor to renal cell carcinoma progression, including invasion and metastasis. (biomedcentral.com)
To explore the effect of miR-296-5p on the metastasis of nasopharyngeal carcinoma (NPC) cells and investigate the underlying mechanism. (biomedcentral.com)
Then, immunofluorescence imaging, scratch wound-healing, transwell migration and invasion assays were used to observe the effects of miR-296-5p on cell metastasis and invasion. (biomedcentral.com)
Finally, TGF-β expression plasmids were transfected into NPC cells to verify the role of TGF-β in the miR-296-5p-mediated inhibition of nasopharyngeal carcinoma cell metastasis. (biomedcentral.com)
This provides new insight into the role of miR-296-5p in regulating NPC metastasis and invasiveness. (biomedcentral.com)
Therefore, their dysregulation may participate in NPC cell invasion and migration, contributing to its progression and metastasis. (biomedcentral.com)
In this study, we established that miR-296-5p has a significantly lower expression in NPC tissues and cells and was involved in regulating the EMT and metastasis of NPC cells in vitro. (biomedcentral.com)
This study revealed a novel mechanism by which miR-296-5p regulates the EMT and metastasis of NPC cells, indicating that it could be a new target for the treatment of NPC. (biomedcentral.com)

The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
NcRNAs are a class of RNA molecules that do not encode proteins, and they play an important role in regulating cellular transcription and post-transcriptional processes [ 7 ]. (ijbs.com)
They function by binding to messenger RNA (mRNA) molecules and inhibiting their translation into protein. (silexon.ai)
ASOs are short synthetic DNA or RNA molecules that can bind to specific mRNA or miRNA targets, preventing their function or promoting their degradation. (silexon.ai)
Chemical modifications to provide drug-like properties to RNA molecules are used in the synthesis of both siRNAs and antimicroRNAs (antimiRs). (genengnews.com)

Regardless of aetiology, the progression of hepatocellular carcinoma (HCC) is highly variable and is influenced by numerous non-genetic factors such as age, diet, and inflammation. (ntu.ac.uk)

Luciferase reporter assay was performed to verify that miR-455 directly binds to the 3′-noncoding region (UTR) of RAB18 mRNA in Huh7 cells. (hindawi.com)
miR-455 inhibited cell viability and invasion by directly targeting the 3′-UTR of RAB18 mRNA of hepatocellular carcinoma. (hindawi.com)
The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. (cancerindex.org)
This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA. (nih.gov)
These naturally occurring, noncoding, single-stranded RNAs (21-25 nucleotides long) base-pair with their target mRNA within the RISC (RNA-induced silencing complex). (genengnews.com)
At last, the mRNA and protein expression of NFAT2, Egr2, FasL, COX-2 and c-myc in carcinoma and adjacent tissues was investigated. (researchsquare.com)

Studies have indicated that MIR374B acts as a tumor suppressor in several cancer types, including gastric cancer, hepatocellular carcinoma, and breast cancer. (silexon.ai)
For instance, RUNX2 is involved in the progression of various tumor types, such as osteosarcoma, renal cell carcinoma, gastric cancer and breast cancer ( 15 - 20 ). (spandidos-publications.com)

The mature microRNA products are thought to regulate expression levels of other genes through complementarity to the 3' UTR of specific target messenger RNA. (wikipedia.org)
Identification of novel genes coding for small expressed RNAs" (PDF). (wikipedia.org)
Hub genes were selected by a protein-protein interaction (PPI) network, and competitive endogenous RNA (ceRNA) networks were constructed by Cytoscape. (researchsquare.com)
ADARs have been shown to contribute to disease pathologies by editing of glutamate receptors, editing of serotonin receptors, mutations in ADAR genes, and by other mechanisms, including recently identified regulatory roles in microRNA processing. (biomedcentral.com)
Over the past few years, emerging numerous bioinformatic tools have been developed to identify candidate disease-causing genes [ 4 ], including microRNA (miRNA) genes. (hindawi.com)
Since strong consensus regarding commonly altered MRs in hepatocellular carcinoma (HCC) is lacking, we generated a compendium of HCC datasets from 21 studies and identified a comprehensive signature consisting of 483 genes commonly deregulated in HCC. (aging-us.com)
Thus, the function of miRNA may ultimately depend on the specific microenvironment of a particular cell type, which can provide different repertoire of target genes. (bvsalud.org)

Renal cell carcinoma (RCC) incidence has increased over the past two decades. (hindawi.com)
Renal cell carcinoma (RCC) accounts for approximately 3% of human malignancies, and its incidence appears to be increasing globally [ 1 ]. (hindawi.com)
although it is derived from cells of the renal tubular epithelium, it has several histological subtypes which differ in their clinical outcome and biological features. (hindawi.com)
2012. "Single-CpG-resolution methylome analysis identifies clinicopathologically aggressive CpG island methylator phenotype clear cell renal cell carcinomas. (esmed.org)
The purpose of this study was to investigate the role and mechanism of hypoxia-induced renal cell carcinoma and provide evidence-based medical proof for improvements to postoperative nursing of renal cell carcinoma patients. (biomedcentral.com)
A total of 64 patients with renal cell carcinoma were divided into the observation group (nursing based on oxygen administration) and the control group (conventional nursing). (biomedcentral.com)
The human renal cell carcinoma cell line A498 under hypoxia/normoxia was used as an experimental model in vitro and the biological characteristics and mitochondrial function of the cells were assessed. (biomedcentral.com)
Nursing based on oxygen administration decreased the value of renal function indexes, serum inflammatory factors, and tumor markers in renal cell carcinoma patients. (biomedcentral.com)
These data reveal that nursing based on oxygen administration can improve the clinical efficacy of renal cell carcinoma therapies, being safe and effective. (biomedcentral.com)
There are three main histological subtypes of RCC, including clear cell RCC (ccRCC), papillary RCC, and chromophobe RCC, which jointly represent over 85% of all primary renal malignancies [ 3 ]. (biomedcentral.com)
The relationship between blood pressure and risk of renal cell carcinoma. (who.int)
Epidemiology of Renal Cell Carcinoma: 2022 Update. (who.int)
Mycotoxin Exposure and Renal Cell Carcinoma Risk: An Association Study in the EPIC European Cohort. (who.int)
Biomarkers of the transsulfuration pathway and risk of renal cell carcinoma in the European Prospective Investigation into Cancer and Nutrition (EPIC) study. (who.int)

A novel oncolytic adenovirus inhibits hepatocellular carcinoma growth[J]. Journal of Zhejiang University Science B, 2019, 20(12): 1003-1013. (zju.edu.cn)
Our results show that miR-296-5p inhibits the migratory and invasive capacities of NPC cells by targeting TGF-β, which suppresses EMT. (biomedcentral.com)

Circulating long non-coding RNAs NKILA, NEAT1, MALAT1, and MIAT expression and their association in type 2 diabetes mellitus. (informaticsstudies.org)

Disruption of the p16/cyclin D1/retinoblastoma protein pathway in the majority of human hepatocellular carcinomas. (journalsmededu.pl)

In a whole-genome sequencing in 17 Esophageal Squamous Cell Carcinoma cases and whole-exome sequencing in 71 cases, Song (2014), reported ASH1L alterations. (cancerindex.org)

Hepatocellular carcinoma (HCC) has been regarded as the fifth most common cancer worldwide with a low prognosis. (hindawi.com)
The complex interplay between them is essential in promoting malignant phenotype, poor prognosis, and drug resistance in GI tumors (including esophageal, gastric, colorectal, liver, and pancreatic cancers). (ijbs.com)
Recent studies reported microRNAs as promising biomarkers for early cancer detection, accurate prognosis, and molecular targets for future treatment. (hindawi.com)
For example, in hepatocellular carcinoma, the downregulation of MIR374B has been associated with advanced disease stages and poor prognosis, making it a potential biomarker for disease progression. (silexon.ai)
For instance, a recent study by our group reported the facilitating effect of RUNX2 during aggressiveness and chemoresistance of TNBC cells via activating MMP1, which was significantly associated with poor prognosis ( 21 ). (spandidos-publications.com)

microRNA profiling revealed potential regulators of the KDM, and preliminary in vitro functional studies propose an oncogenic role of KDM in liver cancer. (ntu.ac.uk)
TIME-related RNA or RNA regulators could be promising targets for anticancer immunotherapy. (biomedcentral.com)

Numbers shown below represent number of publications held in OncomiRDB database for Oncogenic and Tumor-Suppressive MicroRNAs. (cancerindex.org)
The oncogenic communication with tumor cells and through the crosstalk of autocrine and paracrine components in almost all tumor types are responsible for this phenomenon. (biomedcentral.com)
Depending on the context and the cell is type they are expressed, one can view miRNA oncogenic or tumor suppressor activity. (bvsalud.org)

In addition, in the nasopharyngeal carcinoma cell line, miR-20a and miR-20b has been shown to target the 3' UTR of vascular endothelial growth factor (VEGF) and repress the expression of VEGF, which is an important angiogenic factor. (wikipedia.org)

Some of the same strategies already developed for delivery of siRNA for RNA interference (RNAi) also are being applied to miRNA. (genengnews.com)
The previous work has shown that Tanshinone IIA could ameliorate inflammation microenvironment in colorectal cancer via repressing miRNA-155 expression ( 8 ) and exert an anti-angiogenic effect in vascular endothelial cells via inhibiting the VEGF/VEGFR2 pathway [ 9 ]. (biomedcentral.com)
In the THP1 cell line, pri-let-7f levels were increased in response to LPS while the corresponding mature miRNA let-7f levels were reduced. (encyclopedia.pub)
IL-17A is associated with severe asthma and requires IL-23R signaling, which is negatively regulated by let-7f miRNA in CD4+ lymphocytes [ 14 ] . (encyclopedia.pub)
The expressions of miR-296-5p in NPC tissues and cells were determined using GSE32920 database analysis and real-time PCR and miRNA microarray assays. (biomedcentral.com)
Based on our identified miRNA-regulated network, we discovered three drugsBX-912, daunorubicin, and midostaurinthat can be repositioned to treat lung cancer, which was not known before. (cdc.gov)
Based on our identified miRNA-regulated molecular machinery, an inhibitor of PDK1/Akt BX-912, an anthracycline antibiotic daunorubicin, and a multi-targeted protein kinase inhibitor midostaurin were discovered as potential repositioning drugs for treating lung cancer. (cdc.gov)
However, changes in expression of miRNA may be secondary to the tumor phenotype. (bvsalud.org)
This study performs a literature review of miRNA, focusing on aspects related to biogenesis, mechanisms of action and potential role of these small RNAs in oral carcinogenesis. (bvsalud.org)

In addition, miR-455 inhibited cell viability, while it induced cell apoptosis in colorectal cancer [ 13 ]. (hindawi.com)
Annexin V-PE/PI and PI staining were used to analyze cell apoptosis and cell cycle distribution by flow cytometry, respectively. (biomedcentral.com)
The NFAT2 overexpression suppressed the cell viability, invasion and migration capabilities, and promoted apoptosis of HepG2 cells. (researchsquare.com)
The dysfunction is associated with issues with apoptosis and normal cellular metabolic regulation, all regulated through OPA1. (thermofisher.com)

However, ST6GAL1 is also released into the extracellular milieu and extracellularly remodels cell surface and secreted glycans. (nature.com)
We hypothesize that ST6GAL1 action is the combined effect of natively expressed sialyltransferase acting cell-autonomously within the ER-Golgi complex and sialyltransferase from extracellular origins acting extrinsically to remodel cell-surface glycans. (nature.com)
DUSP6/MKP3 is a dual-specific phosphatase that regulates extracellular regulated kinase ERK1/2 and ERK5 activity, with an increasingly recognized role as tumor suppressor. (cancer-genetics.org)

Mechanistically, PTTG3P upregulated the expression of miR-383 targets Cyclin D1 (CCND1) and poly ADP-ribose polymerase 2 (PARP2) by sponging miR-383, acting as a competing endogenous RNA (ceRNA). (biomedcentral.com)

The expression of miR-455 was lower in HCC tissues and cell lines than in nontumor tissues and normal cell line, and downregulation of miR-455 was connected with worse outcome of HCC patients. (hindawi.com)
RAB18 was upregulated in HCC tissues and cell lines, and the expression of RAB18 was regulated by miR-455. (hindawi.com)
All the fresh tissues were snap-frozen in liquid nitrogen and stored at −80°C until RNA extraction. (hindawi.com)
It also significantly decreased the tumor infiltration area and blood vessel density, and increased the percentages of cells with nucleus deformation and cells with condensed chromatin in tumor tissues. (zju.edu.cn)
In this study, we found that PTTG3P was up-regulated in HCC tissues and cells. (biomedcentral.com)
A-to-I editing, which is catalyzed by enzymes of the adenosine deaminase acting on RNA (ADAR) family, is most prevalent in the central nervous system (CNS) but occurs in many tissues [ 1 - 3 ]. (biomedcentral.com)
Compared with adjacent tissues, the carcinoma tissues expressed less NFAT2, Egr2, FasL and more COX-2 and c-myc. (researchsquare.com)
The absence of NFAT2 and Egr2 in carcinoma tissues reminded us that NFAT2 may be a promising therapeutic target for hepatocellular carcinoma treatment. (researchsquare.com)

Insulin receptor substrate 1 (IRS-1) is an adaptor protein that integrates multiple transmembrane signals from growth factors and hormones, to regulate cell growth, survival, differentiation and metabolism ( 4 ). (spandidos-publications.com)
and cell differentiation ( SOX2 and TGFB3 ) as well as immunohistochemical assay for VEGFA, TP53, Bcl2, TGFB1, and Ki67 protein expression have been performed in 85 FFPE RCC tumor specimens. (hindawi.com)
It is involved in p53 pathways and is implicated in cell death/survival signaling, the cell cycle, and differentiation, thereby playing a regulatory role in carcinogenesis [ 12 ]. (hindawi.com)
Nuclear factor of activated T cells 2 (NFAT2, also known as NFATc and NFATc1) is an important member of NFAT family which plays a vital role in T cell activation and differentiation [4]. (researchsquare.com)
To date, RUNX2 has been involved in diverse physiological processes, including osteogenic differentiation of mesenchymal stem/stromal cells, chondrocyte hypertrophy, immunomodulation, vascular invasion and endothelial cell migration via modulating a variety of signaling cascades (e.g. (spandidos-publications.com)
This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. (cancer-genetics.org)

However, miR-455 promoted cell invasion and migration in triple-negative breast cancer [ 14 ]. (hindawi.com)
Transwell migration and invasion assays were used to examine cell migration and invasion abilities. (biomedcentral.com)
Furthermore, NFAT2 participates in the early growth response factor 2 (Egr2)-regulated anergic phenotype which impairs the migration and invasion of leukaemia cells [16]. (researchsquare.com)

MiR-455-5p upregulation in umbilical cord mesenchymal stem cells attenuates endometrial injury and promotes repair of damaged endometrium via Janus kinase/signal transducer and activator of transcription 3 signaling. (nih.gov)

Targeting tumor cells or the tumor microenvironment (TME) are the two major fundamental principles for antitumor therapies. (biomedcentral.com)
The stromal component interacts with tumor cells in complex crosstalk to support tumor growth. (biomedcentral.com)
Investigators have studied DNA methylation in tumor cells for nearly 50 years. (esmed.org)
Tanshinone II A is an effective component extracted from Salvia miltiorrhiza and the roles of Tanshinone IIA in regulating the stemness of tumor cells remain unclear. (biomedcentral.com)

Aim 2: To characterize the molecular mechanisms regulated by KDM. (ntu.ac.uk)
Integration of ChIP-Sequencing with the RNA-Sequencing and metabolic profiling data will reveal the molecular pathways regulated by KDM. (ntu.ac.uk)
Molecular subtypes of small cell lung cancer: a synthesis of human and mouse model data. (cancerindex.org)

ADAR3 contains an arginine-rich domain, shown in pink, which binds single-stranded RNA. (biomedcentral.com)

Tumor xenograft data from Balb/c nude mice demonstrated that HCC cells with high NEK2 expression formed larger tumors than those with low NEK2 expression. (oncotarget.com)
Nuclear factor of activated T cells 2 (NFAT2) has been reported to regulate the development and malignancy of few tumors. (researchsquare.com)

Furthermore, RNA-Sequencing and bioinformatic analysis revealed that metabolic pathways are regulated by the KDM. (ntu.ac.uk)
In addition, Ingenuity Pathway Analysis (IPA) integrated with PCR array data showed that the JAK1/STAT3 pathway was significantly altered in cells overexpressing DEFB1, suggesting this to be one of the pathways by which defensin regulates IAV replication in HBEpCs. (cdc.gov)

MicroRNAs, including MIR374B, are stable in various biological fluids, such as blood and urine, and their expression levels can be quantified using highly sensitive techniques like quantitative polymerase chain reaction (qPCR) or next-generation sequencing. (silexon.ai)

One such molecule that has garnered attention is MIR374B, a microRNA that plays a crucial role in cellular processes and has the potential to be a promising target for therapeutic interventions. (silexon.ai)
For instance, MIR374B has been found to be down-regulated in patients with heart failure, suggesting its potential as a therapeutic target in cardiac diseases. (silexon.ai)

And a recent study demonstrates that Tanshinone IIA restrains migratory and invasive ability of cervix carcinoma stem cells by suppressing the transcriptional activity of YAP (Yes-associated protein) [ 11 ]. (biomedcentral.com)
The study has previously revealed that miR-125b faciliates cell migratory ability breast cancer via targeting STARD13 (StAR-related lipid transfer protein 13) [ 20 ]. (biomedcentral.com)

E2F Transcription Factors in Cancer, More than the Cell Cycle. (journalsmededu.pl)

The recurrence and drug resistance of breast cancer are intractable due to the presence of breast cancer stem cells (BCSCs), which are adequate to initiate tumor formation and refractory to conventional remedies. (spandidos-publications.com)
Of note, other studies have also indicated the involvement of RUNX2 in breast cancer stem cells (BCSCs) and breast cancer progression ( 22 , 23 ). (spandidos-publications.com)

Methods: GP73-SphK1sR-Ad5 was constructed by integrating golgi protein 73 (GP73) promoter and sphingosine kinase 1 (SphK1)-short hairpin RNA (shRNA) into adenovirus serotype 5 (Ad5), and transfecting into HCC Huh7 cells and normal human liver HL-7702 cells. (zju.edu.cn)
In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. (nih.gov)
The viral spike protein is essential for cell receptor binding, cell entry, and viral infection. (molcells.org)
NFAT2, as an important transcriptional promoter, regulates expression of TNF-α, myc proto-oncogene protein (c-myc), cyclooxygenase-2 (COX-2), Fas ligand (FasL) and also generates crosstalks with ERK/MAPK pathway and AKT/GSK3b signaling, which achieves its control of the cell fate [7-12]. (researchsquare.com)

This phenotype points to the epigenetic nature of HCC and highlights the need to further our insight into how epigenetic mechanisms may influence HCC development. (ntu.ac.uk)
Cancer cell-extrinsic mechanisms, which are poorly understood, are also believed to contribute to disease progression and the heterogeneous genetic mutations with diverse presentations. (nature.com)
In this study, we aimed to explore the effect of NFAT2 expression on cell fate of HepG2 cell and its potential mechanisms. (researchsquare.com)

Therefore, in this study, we compared the effects of metformin and the AMPK activator A769662 on MGO-induced DR in mice, as well as evaluated cytotoxicity, mitochondrial dynamic changes and dysfunction in ARPE-19 cells. (bvsalud.org)
We found MGO can induce mitochondrial ROS production and mitochondrial membrane potential loss, but reduce cytosolic ROS level in ARPE-19 cells. (bvsalud.org)
Although these effects of MGO can be reversed by both metformin and A769662, we demonstrated that reduction of mitochondrial ROS production rather than restoration of cytosolic ROS level contributes to cell protective effects of metformin and A769662. (bvsalud.org)
Emerging evidence indicates a putative role of long noncoding RNAs in regulating mitochondrial functions and metabolism. (informaticsstudies.org)
This article discusses the relationship between mitochondrial respiration and long noncoding RNAs and further outlines the implications of these interactions in obesity. (informaticsstudies.org)
Long non‑coding RNA AK055347 is upregulated in patients with atrial fibrillation and regulates mitochondrial energy production in myocardiocytes. (informaticsstudies.org)
It was therefore assumed that cancer cells were generating energy using glycolysis rather than mitochondrial oxidative phosphorylation, and that the mitochondria were dysfunctional. (researchgate.net)

Additionally, we will explore the post-transcriptional regulation of KDM based on our microRNA preliminary data using western blot, RT-qPCR and 3'UTR reporter assays. (ntu.ac.uk)

We found that shRNA knockdown of intrinsic ST6GAL1 expression resulted in decreased ST6GAL1 cargo in the exosome-like vesicles as well as decreased breast tumor cell growth and invasive behavior in 3D in vitro cultures. (nature.com)

RNA editing refers to post-transcriptional processes that alter the nucleotide sequence of an RNA transcript by insertion, deletion or nucleotide conversion. (biomedcentral.com)

In vitro mammary spheroid formation, flow cytometry assay on CD24 − /CD44 + sub-population, ALDH activity detection, cell viability assay and western blot analysis, and in vivo tumor-initiating analysis were performed to examine the effects of Tanshinone IIA on the stemness of breast cancer cells. (biomedcentral.com)
Tanshinone IIA attenuated the stemness of breast cancer cells, evident by downregulating the expression of stemness markers, hindering the capacity of spheroid formation, decreasing the CD24 − /CD44 + sub-population in a concentration-dependent manner and reducing the tumor-initiating ability of breast cancer cells. (biomedcentral.com)
Additionally, Tanshinone IIA enhanced adriamycin sensitivity and attenuated adriamycin resistance of breast cancer cells. (biomedcentral.com)
Notably, miR-125b overexpression enhanced the stemness of breast cancer cells, and miR-125b overexpression or STARD13 knockdown impaired the inhibitory effects of Tanshinone IIA on the stemness of breast cancer cells. (biomedcentral.com)
Let-7f was negatively regulated by estrogen receptor signaling in the MCF-7 breast cancer cell line [ 15 ] . (encyclopedia.pub)

Small cell lung cancer (SCLC) is an exceptionally lethal malignancy for which more effective therapies are urgently needed. (cancerindex.org)
The current study firstly suggested that NFAT2 suppressed the aggression and malignancy of HepG2 cells through inducing the expression of Egr2. (researchsquare.com)

More importantly, we summarize the potential of various RNA-based therapeutics clinically available for cancer treatment. (biomedcentral.com)
RNA-dependent targeting of the TIME, as monotherapy or combined with other evolving therapeutics, might be beneficial for cancer patients' treatment in the near future. (biomedcentral.com)

Cell viability and invasion were measured by CCK8 and Transwell assays. (hindawi.com)
RAB18 reversed partial roles of miR-455 on cell viability and invasion in HCC. (hindawi.com)
Tumor-native ST6GAL1 promotes tumor cell behaviors such as invasion and resistance to cell stress and chemo- and radio-treatments. (nature.com)

Therefore, understanding the TME and its immune cell components are equally important as cancer cell characteristics for tumor eradication. (biomedcentral.com)
Tumor-infiltrating immune cells are an important component of the TIME and are a significant predictor of cancer patients' survival. (biomedcentral.com)
During the tumor initiation stage, cancer cells escape from immune surveillance. (biomedcentral.com)
Using basic science approaches including whole system immune phenotyping with mass cytometry and genetic manipulation of peripheral and central immune cells, we seek to dissect the temporal and tissue-specific contribution of these cells to either promotion or inhibition of healing. (stanford.edu)

We have screened 182 small molecule epigenetic modulators on liver cancer cells and found that inhibitors of a specific histone demethylase (KDM) induce cell death. (ntu.ac.uk)

Moreover, researchers have discovered that MIR374B expression is altered in neurodegenerative disorders like Alzheimer鈥檚 disease, highlighting its role in regulating neuronal health. (silexon.ai)

Furthermore, we showed that DAB2IP negatively regulated ATG9B expression by blocking the Wnt/β-catenin pathway. (nature.com)

SNHG16-miR-23-3p/miR-103-5p/HAPLN1 and XIST-miR-23-3p/ miR-103-5p/HAPLN1 might regulate the disease progression of HF at the transcriptional level. (researchsquare.com)
A-to-I RNA editing is a post-transcriptional modification that converts adenosines to inosines in both coding and noncoding RNA transcripts. (biomedcentral.com)
Eukaryotic RNA transcripts can undergo a range of post-transcriptional modifications, which increase the diversity of the transcriptome without requiring increases in genome size. (biomedcentral.com)

It is localized to many small speckles in the nucleus, and also to cell-cell tight junctions. (cancerindex.org)
E2F2 effects the cell cycle, tumor suppressor proteins, and can also be transformed by proteins of small DNA tumor viruses. (journalsmededu.pl)
To dissect the role of PMN in this context, we pretreated rats with Repertaxin (Rep), a small molecule inhibitor of CXCR1 and CXCR2, to block recruitment and activation of PMN by CXCL1 or CXCL2 after cell transplantation. (p450inhibitors.com)
Role of Dusp6 Phosphatase as a Tumor Suppressor in Non-Small Cell Lung Cancer. (cancer-genetics.org)

A-to-I editing is a mechanism that regulates and diversifies the transcriptome, but the full biological significance of ADARs is not understood. (biomedcentral.com)

MIR374B, also known as hsa-miR-374b-5p, is a specific microRNA involved in various cellular processes. (silexon.ai)

These findings suggest miR-486-5p negatively regulates NEK2, which is a critical prognostic indicator of HCC patient survival after liver transplantation. (oncotarget.com)
In conclusion, the reduction in IAV copy number in DEFB1 overexpressing cells suggests that beta-defensin-1 plays a key role in regulating IAV survival through STAT3 and is a potential target for antiviral drug development. (cdc.gov)

Anatomy11

Organisms7

Diseases53

Chemicals and Drugs89

Analytical, Diagnostic and Therapeutic Techniques and Equipment48

Phenomena and Processes47

Disciplines and Occupations1

Information Science5

Health Care12

Geographicals1

MiRNAs30

Proliferation14

Gene22

Epithelial11

Metastasis13

Molecules5

Progression of hepatocellular carcinoma1

MRNA6

Gastric cancer2

Genes7

Renal14

Inhibits2

Long non-codi1

Human hepatocellular1

Esophageal Squamous Cell Carc1

Prognosis5

Regulators2

Oncogenic3

Nasopharyngeal carcinoma cell1

MiRNA9

Apoptosis4

Extracellular3

Endogenous1

Tissues8

Differentiation6

Migration3

Mesenchymal1

Tumor cells4

Molecular3

Single-stranded1

Tumors2

Pathways2

Polymerase1

Therapeutic2

Migratory2

Transcription1

Cancer Stem2

Protein4

Mechanisms3

Mitochondrial7

Regulation1

ShRNA1

Post-transcriptional processes1

Breast Cancer5

Malignancy2

Therapeutics2

Invasion3

Immune4

Induce1

Neuronal1

Negatively regulated1

Transcriptional3

Small4

Transcriptome1

Cellular1

Survival2