• 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay was executed for determination of effects of piperine on breast cancer cell viability. (ijpsonline.com)
  • Clonogenic assay was implemented for checking the effect of piperine on clonogenic potency of breast cancer cells. (ijpsonline.com)
  • Cell migration and invasion was monitored by transwell chambers assay. (ijpsonline.com)
  • Western blotting assay was implemented to check the levels of phosphatidylinositol 3-kinase and protein kinase B. Results revealed that piperine induced dose reliant cytotoxicity in MCF-7 human breast cancer cells. (ijpsonline.com)
  • Acridine orange/ethidium bromine staining assay revealed that piperine induced apoptosis in MCF-7 cells which was further investigated by Western blotting. (ijpsonline.com)
  • Khz cytotoxicity was measured using an MTT assay and the mitochondrial membrane potential (MMP)-related, calcium-induced generation of reactive oxygen species (ROS) in A549 cells was measured by flow cytometry. (springer.com)
  • The cell survival rate was measured using the principle of water-soluble tetrazolium salt-1 assay, which is a measurement method for cell survival rate. (researchgate.net)
  • Senescence-associated β-galactosidase (SA-β-gal) assay was implemented to dye β-galactosidases (used as a cell aging biomarker) to measure HDF cell aging by UVA. (researchgate.net)
  • We applied an assay that measures the stability of maintenance of an episomal plasmid in human tissue culture cells to screen for new DNA replication factors. (aacrjournals.org)
  • First, the sensitivity and IC 50 values of chidamide and bortezomib in several gastric cancer cell lines (MGC-803, BGC-823, SGC-7901, and MKN45) were measured using the CCK-8 assay. (dovepress.com)
  • Cell viability or clonogenicity was tested by violet crystal assay. (biomedcentral.com)
  • They had appropriate birth weight and all cytometry [ 10 ] or polymerase chain reac- had a dietetic cause for their condition start- tion assay [ 11 ]. (who.int)
  • TYRO3 or AXL gene knockdown reduced cell proliferation/colony formation. (nature.com)
  • The MTT, colony formation, flow cytometry, wound healing, transwell, and western blotting were performed to examine the function of miRNA-149 and FOXC1 in the development of gastric cancer. (hindawi.com)
  • The effects of silibinin on the migration and invasion of oral cancer cells were evaluated using transwell assays. (jcancer.org)
  • Effects of triterpenes from Ganoderma lucidum on protein expression profile of HeLa cells. (springer.com)
  • Ganoderic acid Mf and S induce mitochondria mediated apoptosis in human cervical carcinoma HeLa cells. (springer.com)
  • Molecular docking studies indicated that all three complexes primarily bind to HSA in subdomain IIA with amino acid residues such as Arg218 and Lys199 which are located at the entrance of Sudlow's site I. The in vitro cytotoxicities of complexes 1-3 against HeLa cells showed promising anticancer activity (IC 50 = 3.5, 3.9 and 16.9 μM for 1 , 2 and 3 , respectively). (rsc.org)
  • Cell cycle analysis by flow cytometry with HeLa cells indicated that 1 and 2 induced cell cycle arrest in the G2/M phase while 3 induced arrest in the G0/G1 phase leading to cell death. (rsc.org)
  • The anticancer activity of the two Ni(II) complexes has been evaluated in human cervical (HeLa) cancer and breast cancer (MCF-7) cell lines. (bgu.ac.il)
  • The fluorescent intensity obtained in HeLa cells reveals the generation of ROS by the complexes using DCFH-DA (2,7-dichlorofluorescein diacetate) dye. (bgu.ac.il)
  • Further, flow cytometry analysis was done, suggesting an arrest of the cell cycle in the S phase of the HeLa cells. (bgu.ac.il)
  • Cell cycle progression is faster in cells in which invadopodia are abolished (by Tks5 knockdown), evidenced by earlier induction of cyclins A and B. A close look at the regulators of G1 revealed that the overexpression of p27 kip1 , but not p21 cip1 , causes faster turnover of invadopodia and increased ECM degradation. (biorxiv.org)
  • Functional analysis revealed that knockdown of PTGR1 significantly inhibited proliferation and colony formation by PC cells. (go.jp)
  • The inhibition of cell proliferation was related to arrest of the cell cycle in the G0/G1 phase and increased apoptosis in response to PTGR1 knockdown as indicated by flow cytometry. (go.jp)
  • Moreover, we found that knockdown of Annexin A2 significantly suppressed cell proliferation and invasion of NSCLCs cells. (jcancer.org)
  • Mechanistically, our studies showed that knockdown of Annexin A2 increased the expression of p53, which in turn, induced cell cycle G2 arrest and inhibited epithelial-to-mesenchymal transition (EMT). (jcancer.org)
  • Knockdown of Annexin A2 significantly suppressed cell proliferation and invasion of NSCLCs cells, and the biological effect of Annexin A2 on proliferation and invasion was mediated by the inhibition of p53, which in turn, promoted cell cycle progression and EMT. (jcancer.org)
  • Either USP7 or PLK1 knockdown by RNAi significantly sensitized taxane-resistant cells to taxane cell killing. (biomedcentral.com)
  • The present study confirmed previous observations by others of a progression from G0 through G1 and S phase to G2/mitosis during the first 3 d in culture. (rupress.org)
  • Flow cytometric analysis evidenced that piperine arrested the cell cycle at growth 2/mitosis phase, hence suppressed the breast cancer progression. (ijpsonline.com)
  • While several studies addressed how cell motility parameters change throughout the cell cycle, the relationship between invadopodia and cell cycle progression has not been elucidated. (biorxiv.org)
  • Taken together, these findings suggest that invadopodia function is tightly linked to cell cycle progression and is controlled by specific cell cycle regulators. (biorxiv.org)
  • Cell cycle progression is governed by a complex network of cyclin-dependent kinases that define not only the phase of the cell cycle, but also the timing of transitions between phases 13 . (biorxiv.org)
  • We applied a range of molecular and cellular techniques to measure cell survival, cell cycle progression, protein expression and DNA damage combined with a transcriptomic micro-array approach in D283-MED cells, for global gene expression analysis in acute and chronic hypoxic conditions. (biomedcentral.com)
  • We identify an important role for DDX5 in G 1 -S-phase progression where it directly regulates DNA replication factor expression by promoting the recruitment of RNA polymerase II to E2F-regulated gene promoters. (aacrjournals.org)
  • Taken together, our results suggested that decreased LAPTM5 inhibited proliferation and viability, as well as induced G0/G1 cell cycle arrest possibly via deactivation of ERK1/2 and p38 in BCa cells. (spandidos-publications.com)
  • Both NBT/ATST and TAN/ATST combinations at low doses produced much stronger inhibitory effect on cancer cell viability in comparison to those produced by NBT, TAN, or ATST alone at much higher doses. (umass.edu)
  • In agreement, pharmacological inhibition of MITF signaling with ML329, significantly reduced OMM2.5 cell survival and viability in vitro ( p = 0.0001) and in vivo ( p = 0.0006). (biorxiv.org)
  • In this study, we propose a strategy for the isolation of polyphosphate-accumulating organisms (PAOs) by coupling (i) specific staining of an intracellular accumulate without compromising cell viability, and (ii) rapid detection and sorting of target cells by using fluorescence-activated cell sorting (FACS), for which wastewater sludge samples were utilized. (frontiersin.org)
  • This study is the first to show that VALD-3 induces apoptosis and cell cycle arrest in human breast cancer cells by suppressing Wnt/β-catenin signaling, indicating that it could be a potential drug for the treatment of breast cancer. (researchsquare.com)
  • USP7 inhibition induces cell apoptosis and cell cycle G2/M arrest, and overcomes taxane resistance by inducing the protein degradation of PLK1, resulting in chromosome misalignment in mitosis. (biomedcentral.com)
  • To investigate the effects of silibinin on the growth of oral cancer cells, cell proliferation and anchorage-independent colony formation tests were conducted on YD10B and Ca9-22 oral cancer cells. (jcancer.org)
  • Silibinin effectively suppressed YD10B and Ca9-22 cell proliferation and colony formation in a dose-dependent manner. (jcancer.org)
  • The polyP positive cells were recovered by FACS, purified by colony isolation and phylogenetically identified by 16S rRNA gene sequencing. (frontiersin.org)
  • Herein, we engineered attenuated Salmonella typhimurium VNP20009 with gene circuits to synthetize granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 7 (IL-7) within tumors, which recruited dendritic cells (DCs) and enhanced T cell priming to elicit anti-tumor response. (bvsalud.org)
  • However, MSCs need to be expanded in vitro in order to obtain sufficient cells for clinical trials since they are extremely rare in various tissues. (hindawi.com)
  • Unlike embryonic stem cells, adult stem cells (MSCs) have a limited lifespan and stop proliferating during in vitro culture due to replicative senescence [ 8 ]. (hindawi.com)
  • Investigating FlowSight® imaging flow cytometry as a platform to assess chemically induced micronuclei using human lymphoblastoid cells in vitro. (swansea.ac.uk)
  • This study explores the use of a simple human neurosphere-based in vitro model to characterise the pharmacological and toxicological effects of LiCl and VPA using gene expression changes linked to phenotypic alterations in cells. (plos.org)
  • Human embryonic stem cells (hESc) can also be used to analyze the in vitro development from undifferentiated pluripotent cells leading to terminally differentiated cell types, recapitulating the process of early embryonic development [5] . (plos.org)
  • Therefore, the current study used integrated in vitro and in silico approaches to figure out Amy and Sor's possible synergistic activity in targeting AMPK/mTOR and BCL-2 for anti-angiogenesis and apoptosis cell death in HepG2 cells. (biomedcentral.com)
  • In this study, we evaluate the efficacy of ACY-1215, a histone deacetylase 6 inhibitor (HDAC6i), to attenuate MUM cell growth in vitro and in vivo, and elucidate the underlying molecular mechanisms. (biorxiv.org)
  • Treatment of OMM2.5 MUM cells with ACY-1215 resulted in a significant ( p = 0.0001), dose-dependent reduction in cell survival and proliferation in vitro , and in vivo regression of primary OMM2.5 xenografts in zebrafish larvae. (biorxiv.org)
  • The bacteria-produced GM-CSF stimulated the maturation of bone marrow-derived dendritic cells (BMDCs), while IL-7 promoted the proliferation of spleen isolated T cells and inhibited cytotoxicity T cell apoptosis in vitro. (bvsalud.org)
  • Stem cell senescence in the in vitro expansion stage is an essential concern in clinical procedures of cell therapy. (magiran.com)
  • The risk is increased 1000-fold for squamous cell carcinoma, basal cell carcinoma, malignant melanoma, and fibrosarcoma and is increased 10-fold to 20-fold for other tumors. (medscape.com)
  • NSCLCs including adenocarcinoma, squamous cell carcinoma and large cell carcinoma, constitute 80% of all lung cancers [ 2 ]. (jcancer.org)
  • In our previous study, miRNA-183, a miRNA in the miR-96-182-183 cluster, was significantly over-expressed in esophageal squamous cell carcinoma (ESCC). (molcells.org)
  • Flow cytometry was used to examine apoptosis, cell cycle distribution, and accumulation of reactive oxygen species (ROS). (jcancer.org)
  • Jacobson MD. Reactive oxygen species and programmed cell death. (springer.com)
  • Miyajima, A, Nakashima J, Yoshioka K. Role of reactive oxygen species in cis-dichlorodiammineplatium-induced cytotoxicity on bladder cancer cells. (springer.com)
  • previously described oxidative stress-inducing compounds causing cell cycle delay led to further investigation in the involvement of equisetin and TA-289 in mitochondrial-mediated generation of reactive oxygen species. (vuw.ac.nz)
  • Flow cytometric detection of reactive oxygen species (ROS) generation caused by TA-289 suggests that the compound may induce cell death via ROS production. (vuw.ac.nz)
  • At GSMS, Coico worked as a student research assistant and studied with the known cell biologist Zbyszek Darzynkiewicz, a professor of biochemistry and researcher of cell differentiation and carcinogenesis, and flow cytometry techniques for characterizing epithelial differentiation. (wikipedia.org)
  • Upon completing postdoctoral training, Coico's stated interests were to study the use of flow cytometry to detect risks for colo-rectal cancer (1985), and the growth and differentiation of epithelial cells (1990). (wikipedia.org)
  • In addition, their osteogenic differentiation potential was increased, and genes involved in cell adhesion, FGF-2 signalling, cell cycle, stemness, cell differentiation, and cell proliferation were upregulated, compared to that of the MSCs cultured on uncoated plates. (hindawi.com)
  • The differentiation of mesenchymal stem cells (MSCs) into multiple cell lineages can be exploited as an attractive strategy for cell-based therapy and regenerative medicine [ 1 ]. (hindawi.com)
  • The ECM provides structural and biochemical support to the cells and has various other functions including cell adhesion, cell to cell communication, and differentiation [ 17 , 18 ]. (hindawi.com)
  • It possesses a unique forkhead domain combined with a fragment of the target gene to initiate transcription [ 10 ] and regulates various biological processes such as tumor cell differentiation, cell proliferation, and migration [ 11 ]. (hindawi.com)
  • One complementary therapy could be 're-activating' vasculogenesis (i.e. the differentiation of precursor cells into mature endothelial cells), a mechanism that occurs in the heart during development but is quiescent in adult hearts. (elifesciences.org)
  • Histone deacetylase (HDAC) plays a pivotal role in regulating the level of cellular DNA acetylation and participate in cell growth, differentiation, and apoptosis. (dovepress.com)
  • CD38 is expressed at increasingly higher levels on B cells at each stage of B-cell differentiation, and is then down-regulated on germinal center B cells and mature plasma cells. (thermofisher.com)
  • CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. (thermofisher.com)
  • Triple treatment showed synergism in terms of exhibiting anti-tumorigenic effects by activating the Hippo YAP signaling pathway in the HCT116 cell line. (frontiersin.org)
  • It induced apoptosis, suppressed cell migration and invasion, blocked cell cycle and phosphatidylinositol 3-kinase/ protein kinase B signaling pathway. (ijpsonline.com)
  • VALD-3 also regulated the Wnt/β-catenin signaling pathway in breast cancer cells, inhibiting the activation of downstream molecules. (researchsquare.com)
  • Extracts prepared from Trametes versicolor under the trade name of I'm-Yunity exhibit anti-tumorigenic activities, as supported by inhibition of the proliferation and induction of apoptosis in malignant cells. (spandidos-publications.com)
  • Given the high frequency of DDX5 amplification in breast cancer, our results highlight DDX5 as a promising candidate for targeted therapy of breast tumors with DDX5 amplification, and indeed we show that DDX5 inhibition sensitizes a subset of breast cancer cells to trastuzumab. (aacrjournals.org)
  • We studied whether cellular senescence induced by HDAC inhibitor sodium butyrate in E1a+cHa-Ras -transformed rat embryo fibroblasts (ERas) and A549 human Ki-Ras mutated lung adenocarcinoma cells would enhance the tumor suppressor effect of MEK/ERK inhibition. (aging-us.com)
  • Senescent ERas cells do not develop cytoprotective autophagy upon inhibition of MEK/ERK pathway due to spatial dissociation of lysosomes and autophagosomes in the senescent cells. (aging-us.com)
  • We show that small interfering RNA inhibition of SET8 expression leads to decreased cell proliferation and accumulation of cells in S phase. (rupress.org)
  • This inhibition of AMPK ultimately leads to inhibition of mTOR and thus induces apoptosis in the HepG2 cells. (biomedcentral.com)
  • Pharmacological USP7 inhibition by P5091 retarded cell proliferation and induced cell apoptosis. (biomedcentral.com)
  • USP7 specifically dequbiquitinates and stabilizes both p53 and MDM2 to various degrees, and USP7 inhibition is expected to inactivate MDM2 and activate p53, thereby leading to cell cycle arrest or apoptosis in cancer cells with functional p53 signaling [ 10 ]. (biomedcentral.com)
  • However, the precise role of lcn2 in the cell survival and death has yet to be determined. (jneurosci.org)
  • As a consequence, p53 activation by etoposide was reduced, and cell survival enhanced. (biomedcentral.com)
  • Macroautophagy (hereafter referred to as autophagy) plays an important role in cancer cells survival upon various conditions of intra- and extracellular stress. (aging-us.com)
  • The microenvironment of the cell and the interactions of many growth factors have a role in tumor development and survival [ 2 , 7 ]. (biomedcentral.com)
  • The resistant mutant displayed a higher basal level of ROS production compared to the wild-type parent, indicating that the resistance mutation led to an up-regulation of antioxidant capacity which provides cell survival in the presence of TA-289. (vuw.ac.nz)
  • Our findings provide evidence that ACY-1215 and ML329 are efficacious against growth and survival of MUM cells and are potential therapeutic options for MUM. (biorxiv.org)
  • By overexpressing PLK1, USP7 that had been depleted by RNAi ceased to induce chromosome misalignment in mitosis and again supported cell proliferation and cell survival. (biomedcentral.com)
  • Phase contrast microscopy evidenced some specific morphological modifications indicating apoptosis allied cytotoxicity of piperine like apoptotic bodies, condensed nucleus and membrane blabbing. (ijpsonline.com)
  • In this study, by comparing the activity of normal cell lines and cancer cell lines after treating with resveratrol, it was found that resveratrol has more significant cytotoxicity in cancer cell lines. (mdpi.com)
  • miRNA-149 was underexpressed in gastric cancer tissues and cells, while overexpression of miRNA-149 promoted cell apoptosis, retarded cell cycle, and inhibited proliferation and migration in AGS and MKN28 cells. (hindawi.com)
  • Mesenchymal stem cells (MSCs) possess great therapeutic potential. (hindawi.com)
  • In this study, we investigated a plausible therapeutic synergism of a triple combination of CBD/CBG, curcumin, and piperine in the colon adenocarcinoma using HCT116 and HT29 cell lines. (frontiersin.org)
  • Together our results demonstrate a new mechanism explaining hypoxia-induced resistance involving the alteration of the response to DSB in D283-MED cells, but also highlight the cell type to cell type diversity and the necessity to take into account the differing tumour genetic make-up when considering re-sensitisation therapeutic protocols. (biomedcentral.com)
  • Our results show a novel role for DDX5 in cancer cell proliferation and suggest DDX5 as a therapeutic target in breast cancer treatment. (aacrjournals.org)
  • In conclusion, PTGR1 plays an essential role in PC cells and may be a potential therapeutic target for PC. (go.jp)
  • Taken together, our findings establish the evidence that aberrant activation of Wnt/β-catenin pathway involving an autocrine Wnt singaling drives the proliferation of bone sarcoma cells, and identify the autocrine activation of the Wnt/β-catenin signaling as a potential novel therapeutic target for bone sarcomas. (oncotarget.com)
  • However, patients suffered with tumors that had inadequate infiltrated immune cells only receive limited therapeutic benefits from ICB therapy. (bvsalud.org)
  • Cell-cycle manipulation with vinblastine and 5-bromo-2-deoxyuridine (BUdR) revealed that previous passage through both S phase and mitosis was required for entry into this newly observed late phase. (rupress.org)
  • Further studies showed that P5091 induced cell cycle arrest at G2/M phase, and particularly induced chromosome misalignment, indicating the key roles of USP7 in mitosis. (biomedcentral.com)
  • Our findings revealed that different genetic backgrounds of HCT116 and HT29 cell lines resulted in divergent responses to the combination treatments. (frontiersin.org)
  • FINDINGS: Macaques cycle-phases were associated with fluctuations in systemic immune properties and a type-1 inflammatory T cell response with corresponding CCR5+ memory CD4 T cell (HIV target cell) infiltration into the vaginal lumen at the late luteal phase. (cdc.gov)
  • 0.2 × 10 9 /L), the inflammatory response may be muted, and the usual inflammatory findings of leukocytosis or white blood cells in the urine or at the site of infection may not occur. (msdmanuals.com)
  • It is suggested that curcumin can selectively kill tumor cells through its multifaceted metabolic effects, that culminate in its anti-oxidant and anti-inflammatory activities ( Hewlings and Kalman, 2017 ). (frontiersin.org)
  • However, under these microenvironmental conditions, only 15% of tumor cells assemble invadopodia at any given time, while the rest are non-motile. (biorxiv.org)
  • This can provide Ras-expressing tumor cells additional selective advantages under stress or damage. (aging-us.com)
  • Analysis of the changes of migration and invasion, showed significant reduced LAPTM5 suppressed cell metastasis. (spandidos-publications.com)
  • Tumor cell invasion and metastasis require cancer cells to be both proliferative and invasive, i.e. migrate through the tissue and assemble invadopodia. (biorxiv.org)
  • Metastasis is a complex, multi-step process that is initiated when cancer cells in the primary tumor acquire invasive properties, including motility and the ability to breakdown the extracellular matrix (ECM) 1 , and is responsible for the majority of cancer-related mortalities 2 . (biorxiv.org)
  • Annexin A2 has been involved in cancer cell adhesion, invasion and metastasis. (jcancer.org)
  • Human gastric cancer cell lines AGS and MKN28 were cultured and transfected with miR-149 overexpression plasmid and its control or FOXC1 siRNA and its control. (hindawi.com)
  • The human gastric epithelial permanent cell line (GES-1), human gastric cancer cell lines (AGS, BGC-823, MKN45, and SGC-7901), and HEK293 cells were provided by the Biomedical Experiment Center of Xi'an Jiaotong University. (hindawi.com)
  • The aim of this study was to investigate the antitumor effect of chidamide in combination with bortezomib on gastric cancer cell lines. (dovepress.com)
  • Then, the relatively insensitive gastric cancer cell lines (MGC-803 and BGC-823) were treated with low concentrations of chidamide alone, bortezomib alone, or chidamide and bortezomib combination to detect the effects on cell proliferation, apoptosis, migration, and invasion. (dovepress.com)
  • Our transcriptome analysis revealed in bladder cancer (BCa) tissues a significant induction of lysosomal-associated multispanning membrane protein 5 (LAPTM5), a lysosomal membrane protein preferentially expressing in immune cells and hematopoietic cells. (spandidos-publications.com)
  • Moreover, some studies demonstrated that LAPTM5 was highly expressed in malignant B lymphomas and involved in B cell malignancies ( 10 ), involving in negative regulation of cell surface T and B cell receptor by promoting lysosome degradation ( 6 ). (spandidos-publications.com)
  • Medulloblastoma (MB) is a malignant embryonal brain tumour originating from neural stem cells or granule-cell progenitors of the cerebellum, due to a deregulation of signalling pathways involved in neuronal development such as Wnt or Sonic Hedgehog (SHH) [ 1 ]. (biomedcentral.com)
  • According to recent data, Ras-transformed cells require autophagy to survive and maintain malignant phenotype [ 5 - 10 ]. (aging-us.com)
  • In this study, using invadopodia- and cell cycle- fluorescent markers, we show in 2D and 3D cell cultures, as well as in vivo , that breast carcinoma cells assemble invadopodia and invade into the surrounding ECM preferentially during the G1 phase of the cell cycle. (biorxiv.org)
  • Activation of Wnt/β-catenin signaling with Wnt3a or GSK-3β inhibitor drives the proliferation of bone sarcoma cells, whereas downregulation of activated Wnt signaling with dnTCF4 or siLEF1 suppresses bone sarcoma proliferation and induces cell cycle arrest. (oncotarget.com)
  • Our results caution that this coordination between invasion and cell cycle must be considered when designing effective chemotherapies. (biorxiv.org)
  • SOX9 promotes nasopharyngeal carcinoma cell proliferation, migration and invasion through BMP2 and mTOR signaling. (cancerindex.org)
  • Overexpression of SOX9 promoted NPC cell proliferation, migration and invasion. (cancerindex.org)
  • Compared with low-dose chidamide or bortezomib alone, the low-dose drug combination significantly inhibited the proliferation, migration, and invasion of MGC-803 and BGC-823 cells and induced apoptosis of the cells. (dovepress.com)
  • Our results suggest that the combination of chidamide and bortezomib can significantly reduce the proliferation, invasion, and migration of MGC-803 and BGC-823 cells, providing a framework for the clinical evaluation of combined therapies for gastric cancers. (dovepress.com)
  • A substantial synergistic interaction between Amy and Sor was observed (CI 50 = 0.56), which was connected to cell cycle arrest at the S and G2/M stages and increased apoptosis and potential necroptosis. (biomedcentral.com)
  • In addition, in order to phenotypically anchor the gene expression data, changes in the heterogeneity of cell subtype populations and cell cycle phase were monitored using flow cytometry. (plos.org)
  • Despite their importance for immunity against sexually transmitted infections (STIs), the composition of female reproductive tract (FRT) memory T cell populations in response to changes within the local tissue environment under the regulation of the menstrual cycle remains poorly defined. (cdc.gov)
  • Peripheral blood mononuclear cell (PBMC) samples from healthy women (n=10) were prospectively collected over the course of a menstrual cycle to profile T cell populations. (cdc.gov)
  • The TAM receptor and ligand expression was evaluated in LMS cell lines and 358 sarcoma samples by either gene expression or immunohistochemistry. (nature.com)
  • High expression of TYRO3 and AXL was detected in LMS cell lines. (nature.com)
  • Spatially-resolved profiling of carbon nanotube uptake across cell lines. (swansea.ac.uk)
  • Isobologram analysis confirmed that both NBT/ATST and TAN/ATST combinations produced strong synergy in inhibiting the growth of two breast cancer cell lines. (umass.edu)
  • The purpose of this study was to demonstrate the effects of lycopene, the major tomato carotenoid, on the expression of the BRCA1 and BRCA2 genes in three breast tumour cell lines, MCF-7, HBL-100, MDA-MB-231 and the fibrocystic breast cell line MCF-10a. (nih.gov)
  • We observed an increase of BRCA1 and BRCA2 mRNA in the oestrogen receptor (ER)-positive cell lines (MCF-7 and HBL-100), and a decrease (MDA-MB-231) or no change (MCF-10a) in the ER-negative cell lines. (nih.gov)
  • Fitting this model to flow cytometry data from 5-bromo-2'-deoxyuridine (BrdU) pulse labeling experiments of two different cell lines shows that the mean field predictions mimic closely the measured average kinetics. (hamilton.ie)
  • Two PTGR1-specific short hairpin RNA (shRNA) sequences were used to block the expression of PTGR1 via a lentivirus-mediated system in the androgen-independent PC cell lines DU145 and PC 3. (go.jp)
  • In this study, we showed that Annexin A2 was evidently overexpressed in human NSCLCs cell lines and NSCLCs tissues. (jcancer.org)
  • Pearson correlation analysis further confirmed the significant negative correlation between miR-183 and PDCD4 in both cell lines and in ESCC patients. (molcells.org)
  • Two antiestrogens (ICI 182,780 and 4 hydroxytamoxifen) were tested in 2 ER+ (MCF7, T47D) and 1 estrogennonresponsive, ER- (MDA-MB-231) cell lines. (cdc.gov)
  • In zebrafishes, forced expression of rat lcn2 gene increased the number and thickness of cellular processes in GFAP-expressing radial glia cells, suggesting that lcn2 expression in glia cells plays an important role in vivo . (jneurosci.org)
  • Our results suggest that lcn2 acts in an autocrine manner to induce cell death sensitization and morphological changes in astrocytes under inflammatory conditions and that these phenotypic changes may be the basis of reactive astrocytosis in vivo . (jneurosci.org)
  • Finally, the inhibitory effect of the combined chidamide and bortezomib treatment on MGC-803 cells was verified in vivo through tumor formation experiments in nude mice. (dovepress.com)
  • The role of the mTOR pathway in estrogen response was evaluated in aromatase-expressing MCF7/Aro breast cancer cells by immunoblotting. (aacrjournals.org)
  • Treatment of MCF7/Aro cells with estradiol or androstenedione caused modulation of the mTOR pathway, a phenomenon reversed by letrozole or RAD001. (aacrjournals.org)
  • To this end, effects of p-, m-, and o-acetamidophenol on ER+ (MCF7 and T47D) cell proliferation and on % cells in S (DNA synthesis) phase of the cell cycle were determined. (cdc.gov)
  • In MCF7 and 1470 cells (but not in MDA MB-231 cells), both antiestrogens inhibited proliferation induced by acetaminophen and by estradiol. (cdc.gov)
  • Muse flow cytometric analysis was performed for assessing of different cell cycle phases. (ijpsonline.com)
  • Applications Reported: This 90 antibody has been reported for use in flow cytometric analysis. (thermofisher.com)
  • Applications Tested: This 90 antibody has been tested by flow cytometric analysis of mouse splenocytes. (thermofisher.com)
  • qRT-PCR was used to detect the expression of miRNA-149 and FOXC1 in gastric cancer tissues and cells. (hindawi.com)
  • In the present study, up-regulation of SOX9 was observed in both NPC tissues and different NPC cells. (cancerindex.org)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Both USP7 and PLK1 were overexpressed in taxane-resistant cancer cells, and negatively correlated with the MP scores in tumor tissues. (biomedcentral.com)
  • Our facilities provide the opportunity to study protein structure, molecular probes and drug design, system biology and molecular interactions in cells and tissues. (lu.se)
  • BNP treatment increased vascularisation and the number of endothelial cells in all areas of infarcted hearts. (elifesciences.org)
  • Endothelial cell lineage tracing showed that BNP directly stimulated the proliferation of resident endothelial cells via NPR-A binding and p38 MAP kinase activation. (elifesciences.org)
  • Our results demonstrated that these immature cells have a natural capacity to differentiate into endothelial cells in infarcted hearts. (elifesciences.org)
  • The origin of new endothelial cells (i.e. resident or infiltrating) as well as the underlying mechanism leading to their proliferation (partial endothelial-to-mesenchymal transition [EndMT] or not) have long been debated. (elifesciences.org)
  • Annexin A2 (also called ANXA2) is mainly expressed in endothelial cells. (jcancer.org)
  • We studied the cell cycle of lectin-stimulated human lymphocytes, making use of a flow cytometer. (rupress.org)
  • By assaying their anti-proliferative and anti-apoptotic effects using human promyelocytic HL-60 cells, we found that the ethanolic extract of I'm-Yunity-Too was more active in inducing cell death compared to I'm-Yunity, based on measured changes in the expression of caspase 3 and Bax. (spandidos-publications.com)
  • Induction of apoptosis by ethanol extracts of Ganoderma lucidum in human gastric carcinoma cells. (springer.com)
  • Indeed, both the hESc and human neuroprogenitor cell (hNPC) models provide valid and useful tools for studying DNT. (plos.org)
  • Understanding how DNA replication is regulated in human cells can provide insight into cancer development and may reveal vulnerabilities that can be exploited therapeutically. (aacrjournals.org)
  • Herein, we studied NBT and TAN in combination with atorvastatin (ATST, Lipitor, a cholesterol-lowering drug) in MDAMB231 and MCF-7 human breast cancer cells. (umass.edu)
  • In conclusion, our result demonstrated strong synergy between two major citrus PMFs (NBT and TAN) and ATST in inhibiting human breast cancer cell growth. (umass.edu)
  • In this study, we investigated the cytotoxic and apoptotic effects of VALD-3, a Schiff base ligand synthesized from o-vanillin derivatives, on human breast cancer cells and the possible underlying mechanisms. (researchsquare.com)
  • 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)-test was used to observe the proliferation of human breast cancer MCF-7 and MDA-MB-231 cells induced on VALD-3. (researchsquare.com)
  • By xenografting human breast cancer cells into nude mice, we found that VALD-3 significantly suppressed tumor cell growth while showing low toxicity against major organs. (researchsquare.com)
  • Background: Since cell-mediated infection of human immunodeficiency virus type 1 (HIV-1) is more efficient than cell-free infection, cell-to-cell propagation plays a crucial role in the pathogenesis of HIV-1 infection. (pdgfrsignaling.com)
  • human breast cancer cells. (cdc.gov)
  • This study aimed to investigate the anti-proliferative effects of Khz on A549 lung cancer cells. (springer.com)
  • Khz inhibited cell division and induced apoptosis in A549 cells. (springer.com)
  • Flow cytometry analysis showed that the percentage of A549 cells in sub-G1 phase of the cell cycle increased in response to Khz treatment. (springer.com)
  • The generation of a mutant strain resistant to TA-289 also displayed resistance to a known oxidant, H2O2, at concentrations that were cytotoxic to wild-type cells. (vuw.ac.nz)
  • cells and uninfected target cells and infectious synapses between uninfected dendritic cells (DC) harboring HIV-1 and uninfected target cells. (pdgfrsignaling.com)
  • LAPMT5 is a lysosomal membrane protein preferentially expressed in immune cells ( 5 , 6 ) and hematopoietic cells ( 7 ), having a close interaction with the Nedd4 ( 8 ), a member of the E3 ubiquitin ligases family ( 8 ). (spandidos-publications.com)
  • LCN2 protein induced upregulation of glial fibrillary acidic protein (GFAP), cell migration, and morphological changes similar to characteristic phenotypic changes termed reactive astrocytosis. (jneurosci.org)
  • Primig, M.: Integrated RNA- and protein profiling of fermentation and respiration in diploid budding yeast provides insight into nutrient control of cell growth and development. (tu-dresden.de)
  • The protein encoded by this gene, together with spectrin and actin, constitute the red cell membrane cytoskeletal network. (cancerindex.org)
  • In the canonical Wnt/β-catenin signaling, a Wnt ligand binds to a cell surface receptor complex consisting of the members of Frizzled family and the transmembrane protein LRP 5 and 6 to prevent phosphorylation and degradation of β-catenin by the GSK3β/APC/Axin destruction complex. (oncotarget.com)
  • Since Sp100 is a primary intranuclear target of infected-cell protein 0 (ICP0), an immediate early E3 ligase encoded by herpes simplex virus 1 (HSV-1), previous investigations attempting to analyze the functions of individual Sp100 variants during HSV-1 infection mostly avoided using a wild-type virus. (bvsalud.org)
  • USP7 was originally identified as a binding partner for the herpes simplex virus (HSV) infected cell protein and named herpes-associated ubiquitin-specific protease (HAUSP) [ 6 ]. (biomedcentral.com)
  • Flow cytometric estimation of the apoptotic marker CD95 in peripheral neutrophils, lymphocytes and monocytes was done for 18 infants with non-oedematous protein energy malnutrition (PEM) and 12 oedematous ones, on hospital admission and after supervised nutritional rehabilitation, and compared with 12 matched controls. (who.int)
  • In addition to infrastructures for bioimaging, protein and genes & cells, we also provide other resources e.g., databases, networks and specialized labs. (lu.se)
  • Potential synergistic effects of various combinations including these compounds were tested by measuring cancer cell proliferation and apoptosis. (frontiersin.org)
  • Similar effects observed with equisetin treatment suggest that both compounds share the same mechanism, eliciting cell death via ROS production in the mitochondrial respiratory chain. (vuw.ac.nz)
  • However, methods to analyze accumulation of specific compounds in individual living cells from a community, followed by recovery and isolation, are still limited. (frontiersin.org)
  • MSCs on PLL-coated plates exhibited a faster growth rate with increased S-phase and upregulated expression of the stemness markers. (hindawi.com)
  • This revealed increased expression of B-cell lymphoma 2 associated X and reduced expression of B-cell lymphoma 2, indicating apoptosis induction by piperine. (ijpsonline.com)
  • Using transcriptomic analysis in D283-MED cells, we found a large transcriptional remodelling upon long term hypoxia, in particular the expression of a number of genes involved in detection and repair of double strand breaks (DSB) was altered. (biomedcentral.com)
  • Flow cytometry analysis showed a G(1)/S phase cell cycle-arrest after treatment of the cells with 10 microM lycopene for 48 h. mRNA expression was studied by quantitative reverse transcription-polymerase chain reaction using the Taqman method. (nih.gov)
  • Its expression is reported on a subpopulation of thymocytes, mature T cells, and NK cells. (thermofisher.com)
  • While virological synapses are driven by expression of the viral envelope glycoprotein on the cell surface, little is known about selleck the role of envelope glycoprotein during contact between DC and T cells. (pdgfrsignaling.com)
  • Western blot analysis showed that the expression of programmed cell death 4 (PDCD4), which was predicted as the target gene of miR-183 by microarray profiling and bioinformatics predictions, decreased when miR-183 was over-expressed. (molcells.org)
  • The oscillation frequency of progesterone concentration and CCR5 expression on CD4 T cells was calculated using the Lomb-Scargle periodogram. (cdc.gov)
  • Our results indicated that lcn2 is critical for cell death sensitization, stimulation of cell migration, and morphological changes of reactive astrocytes. (jneurosci.org)
  • Treatment of control ERas cells with PD0325901 for 24 h results in mitochondria damage and apoptotic death of a part of cellular population. (aging-us.com)
  • Our results suggest a determinant role for immune activation driven by mDC-CD4(+) T-cell contacts in viral dissemination and that this activation likely contributes to the pathogenesis of HIV-1 infection. (pdgfrsignaling.com)
  • These results indicate that acupuncture at HT8 can inhibit hippocampal cell death and suppress KA-induced inflammatory events, suggesting a possible role for acupuncture in the treatment of epilepsy. (pdgfrsignaling.com)
  • In this study, we investigated the inhibitory effects of miR-149 on the biological functions of gastric cancer cells by targeting FOXC1. (hindawi.com)
  • Western blotting revealed that silibinin downregulated SOD1 and SOD2 and triggered the JNK/c-Jun pathway in oral cancer cells. (jcancer.org)
  • Silibinin considerably reduced the development of oral cancer cells by inducing apoptosis, G 0 /G 1 arrest, ROS generation, and activation of the JNK/c-Jun pathway. (jcancer.org)
  • Further, application of piperine on cancer cell colonies remarkably reduced the number of cell colonies to minimum. (ijpsonline.com)
  • In conclusion, the current investigation regarding anticancer effects of piperine against breast cancer revealed remarkable suppression of cancer cells. (ijpsonline.com)
  • Khz, a naturally occurring compound derived from the fusion of Ganoderma lucidum and Polyporus umbellatus mycelia, inhibits the growth of cancer cells. (springer.com)
  • Zhao S, Ye G, Fu G, Cheng JX, Yang BB, Peng C. Ganoderma lucidum exerts anti-tumor effects on ovarian cancer cells and enhances their sensitivity to cisplatin. (springer.com)
  • Resveratrol could play a toxic role through inducing apoptosis of the cancer cell in a time- and concentration-dependent manner. (mdpi.com)
  • Deregulated cell cycle is a hallmark of cancer 12 . (biorxiv.org)
  • We find that the DDX5 locus is frequently amplified in breast cancer and that breast cancer-derived cells with amplification of DDX5 are much more sensitive to its depletion than breast cancer cells and a breast epithelial cell line that lacks DDX5 amplification. (aacrjournals.org)
  • Defects in the control of cell proliferation are a hallmark of cancer, and DNA replication is a key process for cell proliferation. (aacrjournals.org)
  • Cancer cells exploit a variety of protective mechanisms that allow them to acquire selective advantage and proliferate under unfavorable conditions. (aging-us.com)
  • Despite a large number of promising inhibitors for Ras/Raf/MEK/ERK pathway, recent works show that cancer cells often develop an autophagy-dependent resistance to inhibitors of Ras pathway [ 16 ]. (aging-us.com)
  • One of the mechanisms of high basal autophagy activity in cancer Ras-expressing cells might be associated with high PP2A phosphatase activity targeting directly ULK1-Ser757 that could explain apparent contradiction: maintenance of high mTORC1 functions and high autophagic activity simultaneously [ 22 ]. (aging-us.com)
  • Flow cytometry analysis showed that VALD-3 triggered cell cycle arrest and induced apoptosis of breast cancer cells. (researchsquare.com)
  • This first or primary UM is treated by targeting the cancer cells using ocular radiation implants or by surgical removal of the eye. (biorxiv.org)
  • 13 Genome Plasticity and B Cell, University of Paris-Sud, Centre National de la Recherche Scientifique, Cancer Institute Gustave Roussy, Villejuif, France. (jci.org)
  • In the present study, we explored the oncogenic roles of miR-183 in ESCC by gain and loss of function analysis in an esophageal cancer cell line (EC9706). (molcells.org)
  • In addition to the aging phenomenon, stem cell senescence has been investigated in various concepts such as cancer, adverse drug effects, and as a limiting factor in cell therapy. (magiran.com)
  • We searched the databases such as EMBASE, PubMed, and Web of Science with the keywords "stem cell," "progenitor cell," "satellite," "senescence" and excluded the keywords "cancer," "tumor," "malignancy" and "carcinoma" until June 2020. (magiran.com)
  • The result also indicates that the apoptotic cell death in the cancer cell might be triggered by the process of ROS generation with the complexes. (bgu.ac.il)
  • Similarities between acetaminophen- and estradiol-induced proliferation of cultured, estrogen-responsive breast cancer cells. (cdc.gov)
  • Cellular senescence, which is morphologically characterized by an enlarged and flattened cell shape, was first described by Hayflick [ 9 ]. (hindawi.com)
  • Cellular senescence refers to active cells that eventually enter a state of irreversible growth arrest. (hindawi.com)
  • Inter-cellular variability in the duration of the cell cycle is a well documented phenomena which has been integrated into mathematical models of cell proliferation since the 70's. (hamilton.ie)
  • Here I present a minimalist stochastic cell cycle model that allows for inter-cellular variability at the level of each single phase, i.e. (hamilton.ie)
  • To resolve this uncertainty a novel experimental proto col is proposed able to provide sufficient information about cell kinetics to fully determine both the inter-cellular average and variance of the duration of each of the phases. (hamilton.ie)
  • In this study, we developed a strategy for the isolation of polyphosphate-accumulating organisms (PAOs) by combining (i) nontoxic fluorescence staining of polyphosphate granules in viable microbial cells and (ii) fluorescence-activated cell sorting (FACS) for the rapid detection and collection of target cells. (frontiersin.org)