AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsTechnology, Industry, AgricultureNamed GroupsHealth Care
Coxiella burnetiiCoxiellaQ FeverGoat DiseasesAntibodies, BacterialGoatsSheep DiseasesEndocarditis, BacterialZoonosesDNA, BacterialRickettsia typhiSeroepidemiologic StudiesComplement Fixation TestsRickettsiaVacuolesAbortion, VeterinaryRickettsia rickettsiiVeterinariansPefloxacinHeart ValvesDoxycyclineBartonella quintanaDairyingTicksLivestockMilkChlamydophilaBartonellaPolymerase Chain ReactionCattle DiseasesBlotting, SouthwesternAntigens, BacterialBacterial VaccinesEnvironmental MicrobiologyRickettsia InfectionsFluorescent Antibody Technique, IndirectBacterial Secretion Systems
InfectionBacteriumSerumBacteriaZoonosisGenotyping of CoxiellaOrganismDetectionMolecularGram-negativeObligate intracellularELISAAntibodyCharacterizationHumansVacuole formationCausativeZoonoticAerosolsAgent of Q feverPrevalenceGoatsIsolatesAxenic mediaAntigensNucleicPathogenEndemicStrainPersistsVaccinesSeroprevalenceIllnessMilk SamplesDistinguishImmuneMycobacteriaNine MileTissuesOccursGoatEnzymeEvidenceAcute phase
Infection24
  • in many cases serological assay confirmed the activity of Coxiella burnetii infection. (medscimonit.com)
  • These results suggest the possibility of the involvement of Coxiella burnetii infection in the evolution of chronic fatigue syndrome. (medscimonit.com)
  • Hence, all isolates were genetically identical, and the infection in animals could originate from one Coxiella burnetii strain (Nine Mile RSA493 strain). (ijmm.ir)
  • Coxiella progenies prepared from inhibitor-treated cells retain the capability of normally infecting all tested cells in the absence of the inhibitor, which suggests a dispensable role of lipid A for infection and early vacuole development. (duke.edu)
  • Conclusion Evidence of C. burnetii infection in beef cattle has public health implications for occupational exposure of primary producers and veterinarians and for the proximity of beef cattle properties to residential areas in regional Queensland. (qld.gov.au)
  • Inhalation of low doses of Coxiella bacteria can result in infection of the host alveolar macrophage (AM). However, it is not known whether a subset of AMs within the heterogeneous population of macrophages in the infected lung is particularly susceptible to infection. (montana.edu)
  • An enzyme-linked immunosorbent assay (ELISA) will be used to initially screen all sera specimens for IgG Phase II antibody seropositivity, a marker of acute infection. (cdc.gov)
  • This study indicates that seroprevalence of C. burnetii in Boer goats raised in Missouri was low, but it does not preclude the existence of a higher level of infection in Missouri's meat goat herds. (biomedcentral.com)
  • More research is warranted to corroborate the preliminary findings reported here in order to determine the public health significance C. burnetii infection risks associated with contemporary goat production systems in the US. (biomedcentral.com)
  • A review of the burden of Q fever in the US revealed a 41.6% average prevalence of C. burnetii infection in goats [ 9 ]. (biomedcentral.com)
  • [ 1 ] In acute infection, a phase II IgG antibody response is dominant and higher than the antibody response to phase I. The reverse is true in chronic infection. (medscape.com)
  • PCR, immunohistochemistry, or culture of affected tissue can provide definitive confirmation of infection by Coxiella burnetii. (medscape.com)
  • Detection of C. burnetii DNA by polymerase chain reaction (PCR) can rapidly confirm an acute Q fever infection. (cdc.gov)
  • Q fever is a rare zoonotic infection caused by Coxiella burnetii . (hindawi.com)
  • Q fever is a rare zoonotic rickettsiosis caused by infection with C. burnetii . (hindawi.com)
  • IgM antibodies against phase II antigen are the marker of the acute infection while high levels against phase I antigen indicate a chronic infection. (hindawi.com)
  • The animal infection, also called coxiellosis, is characterized by a subclinical phase with a relatively rare and sudden epidemic appearance of abortion. (idexx.com)
  • La fièvre Q est-elle une infection émergente en Turquie? (who.int)
  • C. burnetii is also maintained in nature through an animal-tick cycle, but arthropods are not involved in human infection. (msdmanuals.com)
  • Molecular detection of Coxiella burnetii in the sera of patients with Q fever endocarditis or vascular infection. (wakeupuganda.org)
  • Survivance of infected cells is important for chronic infection with C. burnetii. (iimmun.ru)
  • During infection C. burnetii translocates effector substrates from bacterial cytosole to euca ryotic host cell cytosole using type IV secretion system, where effectors modulate host cell proteins. (iimmun.ru)
  • Q fever (infection with Coxiella burnetii ) has been uncommon in Australia's Northern Territory, with no reported cases until 2002. (health.gov.au)
  • Documented cases of infection with Coxiella burnetii (Q fever), a notifiable zoonotic disease, have been uncommon in the Northern Territory. (health.gov.au)
Bacterium5
Serum7
  • Background SERION ELISA classic Coxiella burnetii tests are recommended for the detection of human antibodies in serum or plasma directed against Coxiella burnetii in Phase 1 or Phase 2. (qedbio.com)
  • Rolain JM , Mallet MN , Raoult D . Correlation between serum doxycycline concentrations and serologic evolution in patients with Coxiella burnetii endocarditis. (cdc.gov)
  • Methods Serum samples were tested by ELISA for both phase II and phase I antigens of the organism using an Australian isolate. (qld.gov.au)
  • Banked serum samples were secondarily tested for C. burnetii specific antibodies. (biomedcentral.com)
  • However, in chronic Q fever patients, the antiphase I titers exceed those of anti-phase II titers, and patients with chronic Q fever endocarditis can have high levels of serum IgA. (vircell.com)
  • If acute Q fever is suspected, physicians should order IgG IFA (indirect immunofluorescence assay) testing for both phase I and phase II antibodies in paired serum specimens, taken 3-6 weeks apart. (medscape.com)
  • The IDEXX Q Fever Ab Test is an enzyme immunoassay for the detection of antibodies against Coxiella burnetii in serum, plasma and milk samples of ruminants. (idexx.com)
Bacteria6
  • Anyone can get the disease if they are infected with C. burnetii bacteria. (floridahealth.gov)
  • Dust becomes contaminated from the tissues or bodily fluids of animals infected with C. burnetii bacteria. (floridahealth.gov)
  • Unlike many bacteria, C. burnetii replication in axenic media and non-phagocytic cells was less dependent on normal lipid A biosynthesis. (duke.edu)
  • There is evidence that C. burnetii is a public health hazard in the US with humans being exposed to the bacteria through milk. (biomedcentral.com)
  • Coxiella burnetii , the only member of an intracellular bacteria genus that is related to the Rickettsia genus, causes Q fever. (tewhatuora.govt.nz)
  • After internalization of bacteria maturation of phagolysosome-like compartment and large coxiella-containing vacuole formation occure, and vacuole can occupy nearly the whole cytoplasm of the host cell. (iimmun.ru)
Zoonosis2
Genotyping of Coxiella1
Organism1
  • Q fever is a zoonotic disease with acute and chronic stages caused by the rickettsia-like organism Coxiella burnetii. (cdc.gov)
Detection3
  • SERION ELISA classic Coxiella burnetii IgM is recommended for the detection of acute Q-fever, while SERION ELISA classic Coxiella burnetii (Phase 2) IgG supports the differential diagnosis of infections of the respiratory tract, especially atypical pneumonia. (qedbio.com)
  • Detection of antibodies to Coxiella burnetii, the causative agent of Q fever, by ELISA. (tamu.edu)
  • Demonstration of C. burnetii in a clinical specimen by detection of antigen or nucleic acid. (cdc.gov)
Molecular3
  • Lamas CC , Ramos RG , Lopes GQ , Santos MS , Golebiovski WF , Weksler C , Bartonella and Coxiella infective endocarditis in Brazil: molecular evidence from excised valves from a cardiac surgery referral center in Rio de Janeiro, Brazil, 1998 to 2009. (cdc.gov)
  • Molecular characterization of Coxiella burnetii isolates by infrequent restriction site-PCR and MLVA typing. (ijmm.ir)
  • Identification and characterization of novel virulence factors it is now possible through axenic media for C. burnetii cultivation and development of site-specific mutagenesis and other genetic technics, which is important for research of C. burnetii molecular pathogenesis. (iimmun.ru)
Gram-negative1
  • Coxiella burnetii is an obligate intracellular gram-negative bacterial pathogen, an ethiological agent of Q-fever, a zoonotic disease, elapsing as an acute (mostly atypical pneumonia) or a chronic (mostly endocarditis) form. (iimmun.ru)
Obligate intracellular1
  • Coxiella burnetii , an obligate intracellular pathogen of both humans and animals is the causative agent of Q-fever [ 1 ]. (biomedcentral.com)
ELISA8
  • Description SERION ELISA classic Coxiella burnetii Phase 1 IgG/IgA resp. (qedbio.com)
  • SERION ELISA classic Coxiella burnetii (Phase I) tests are recommended for the diagnosis of chronic Q-fever. (qedbio.com)
  • All SERION ELISA classic Coxiella burnetii are used for the serological therapy follow-up in acute and chronic diseases. (qedbio.com)
  • Panel includes Leptospira Panel - 5 Serovars (MAT), Toxoplasma IgG (IFA), Coxiella burnetti (ELISA), Bluetongue Virus (AGID), and Brucella abortus/suis (Card Agglutination). (tamu.edu)
  • 2018) Valoración de un nuevo ensayo quimioluminiscente en comparación con ELISA en la detección de IgG contra el virus de la hepatitis E. Revista de la Sociedad Andaluza de Microbiología y Parasitología Clínica. (vircell.com)
  • Any sera samples positive by ELISA will then be tested by immunofluorescence antibody assay (IFA) in order to obtain end point titers for IgG to both phase I and phase II antigens. (cdc.gov)
  • If the ELISA result was positive or equivocal, Phase 1 and Phase II IFA titers were performed. (cdc.gov)
  • Use of ELISA testing allows for the serological diagnosis of Q fever by detecting C. burnetii -specific antibodies. (idexx.com)
Antibody6
  • An advantage of the indirect fluorescent antibody assay is the ability to use phase I and phase II antigens. (vircell.com)
  • A fourfold increase in phase II immunoglobulin G (IgG) antibody titer by immunofluorescent assay (IFA) of paired acute and convalescent specimens is the diagnostic gold standard to confirm diagnosis of acute Q fever. (medscape.com)
  • Setiyono A , Subangkit M, Marea W, Santi VD, Elvira E, Fadhilah M, Aufa S. 2013. Anti Coxiella burnetii Antibody Specific for Q Fever Diagnosis Immunohistochemically in Ruminant. Proceeding of International Seminar on the Role of Veterinary Science to Support Millennium Development Goals and the 12th Asian Association of Veterinary Schools Congress. (ipb.ac.id)
  • Specimens screened for Phase I antibody at 1:16. (cdc.gov)
  • seroconversion or ≥4-fold increase in antibody level to phase II antigen in paired sera tested in parallel in the absence of recent Q fever vaccination. (tewhatuora.govt.nz)
  • single raised convalescent IgG antibody to phase II antigen. (tewhatuora.govt.nz)
Characterization1
  • Here we report the characterization of the role of lipid A in Coxiella burnetii growth in axenic media, monkey kidney cells (BGMK and Vero), and macrophage-like THP-1 cells by using a potent LpxC inhibitor -LPC-011. (duke.edu)
Humans3
  • Several methods have been employed to identify Coxiella burnetii isolates based on the specific Coxiella burnetii QpH1 plasmid to distinguish the acute form from the chronic form of Q fever disease in humans and animals owing to the presence of unique gene sequences in this plasmid. (ijmm.ir)
  • Coxiella burnetii, the causative agent of Q fever, is a zoonotic disease with potentially life-threatening complications in humans. (montana.edu)
  • Humans acquire C. burnetii by inhaling contaminated aerosols or dust generated by placental tissues, birth fluids or excreta of infected animals. (tewhatuora.govt.nz)
Vacuole formation2
  • In inhibitor-treated THP-1 cells, Coxiella shows severe growth defects characterized by poor vacuole formation and low growth yields. (duke.edu)
  • Except that C. burnetii involves autophagic pathway during coxiella-containing vacuole formation, and induction of autophagy promotes pathogen replication. (iimmun.ru)
Causative1
  • The aim of this study was to undertake a cross-sectional seroprevalence survey of Coxiella burnetii, the causative agent of Q fever, in beef cattle in Queensland. (qld.gov.au)
Zoonotic1
Aerosols1
  • C. burnetii persists in stool, urine, milk, and tissues (especially the placenta), so that fomites and infective aerosols form easily. (msdmanuals.com)
Agent of Q fever1
Prevalence3
  • However, there has been very little research on the prevalence of C. burnetii exposure and risk in meat goats farmed in the US. (biomedcentral.com)
  • No studies have evaluated the prevalence and risk factors of C. burnetii infections in meat goats farmed in Missouri. (biomedcentral.com)
  • C. burnetii has a worldwide occurrence with an increased prevalence in countries with dense cattle, sheep and goat populations. (idexx.com)
Goats3
  • Raw or unpasteurized milk from infected cows or goats may be capable of spreading C. burnetii . (floridahealth.gov)
  • The aim of this pilot investigation was to determine whether Boer goats, the preponderant meat goat breed in Missouri, were exposed to C. burnetii and to estimate seroprevalence of the exposure. (biomedcentral.com)
  • C. burnetii has a reservoir in birds and mammals, especially cattle, sheep and goats, and is most often an occupational disease affecting farmers, veterinarians and abattoir workers. (tewhatuora.govt.nz)
Isolates3
  • A total of 86 isolates of Coxiella burnetii QpH1 plasmid, which were confirmed by the Nested-PCR method in 2018, were used to determine the RFLP panel of the QpH1 plasmid. (ijmm.ir)
  • 3. Hendrix LR, Samuel JE, Mallavia LP. Differentiation of Coxiella burnetii isolates by analysis of restriction-endonuclease-digested DNA separated by SDS-PAGE. (ijmm.ir)
  • Specific sec reted proteins for variety of strains and isolates were identified, confirmed that certain pathotypes of C. burnetii can exist. (iimmun.ru)
Axenic media2
  • Scholars@Duke publication: Lipid A Has Significance for Optimal Growth of Coxiella burnetii in Macrophage-Like THP-1 Cells and to a Lesser Extent in Axenic Media and Non-phagocytic Cells. (duke.edu)
  • Under inhibitor treatment, Coxiella has reduced growth yields in axenic media and during replication in non-phagocytic cells, and has a reduced number of productive vacuoles in such cells. (duke.edu)
Antigens5
  • Potential Q fever vaccine antigens include lipopolysaccharide (LPS) and several C. burnetii surface proteins. (ox.ac.uk)
  • Antibodies elicited by purified C. burnetii lipopolysaccharide (LPS) correlate with protection against Q fever, while antigens encoded by adenoviral vectored vaccines can induce cellular immune responses which aid clearing of intracellular pathogens. (ox.ac.uk)
  • Multiple vaccine constructs encoding single or fusion antigens from C. burnetii were synthesised. (ox.ac.uk)
  • This study is the first known investigation of C. burnetii seroprevalence in beef cattle in Queensland and the first known use of an Australian C. burnetii isolate for screening using both phase II and phase I antigens. (qld.gov.au)
  • The two antigenic forms of C. burnetii that are important for serologic diagnosis of Q fever are the phase I (i.e. virulent microorganism with smooth LPS [S-LPS]) and phase II (i.e. avirulent microorganism with rough LPS [R-LPS]) whole-cell antigens. (vircell.com)
Nucleic1
  • The results of the nucleic acid sequencing of all 4 samples indicated that they had a Coxiella burnetii type (Nine Mile RSA493 strain). (ijmm.ir)
Pathogen1
  • C. burnetii promotes maturation of specific phagolysosome-like compartment in host cell, called coxiella-containing vacuole, within this vacuole pathogen becames metabolically activated and actively replicates. (iimmun.ru)
Endemic1
Strain1
  • Inhibiting lipid A biosynthesis in C. burnetii by the inhibitor was shown in a phase II strain transformed with chlamydial kdtA. (duke.edu)
Persists1
  • Coxiella persists as metabolically inactive spore-like form in environment. (iimmun.ru)
Vaccines1
  • Immunisation with purified Coxiella burnetii phase I lipopolysaccharide confers partial protection in mice independently of co-administered adenovirus vectored vaccines. (ox.ac.uk)
Seroprevalence2
  • The animal and herd-level seroprevalence estimates for C. burnetii were 1.2% (3/249) and 4.2% (1/24) respectively. (biomedcentral.com)
  • This result is inconclusive because this study was disadvantaged by the small number of individual animal and herds tested, which compromised the statistical power of this study to detect a possible higher seroprevalence of C. burnetii in this population, if present. (biomedcentral.com)
Illness3
  • Two patients were treated with doxycycline for two weeks in the acute phase of illness and one with a combination of erythromycin and gentamycin. (medscimonit.com)
  • IgM antibodies appear since the second week until the fourth month of the acute phase of illness. (vircell.com)
  • The guidelines address treatment of acute and chronic phases of Q fever illness in children, adults, and pregnant women and the management of occupational exposures. (medscape.com)
Milk Samples3
  • Khademi P, Ownagh A, Mardani K, Khalili M. PCR-RFLP of Coxiella burnetii Plasmids Isolated from Raw Milk Samples in Iran. (ijmm.ir)
  • A recent report found that 94% of bulk tank milk samples collected from US dairy herds contained C. burnetii specific DNA [ 6 ]. (biomedcentral.com)
  • Loftis and others detected C. burnetii in 42.9% (9/21) of commercial raw milk samples in the US [ 7 ], and a recent case report found Q fever clusters among raw milk consumers in the State of Michigan [ 4 ]. (biomedcentral.com)
Distinguish1
  • Determining antibodies against phase I and phase II C. burnetii can help distinguish acute and chronic Q fever. (vircell.com)
Immune2
  • In opposition to phase 2, phase 1 Coxiella burnetii trigger a high immune response by activating both cell (T lymphocytes) and humoral (B lymphocytes) immune responses. (ceva.pro)
  • TNF- α has an important role in the immune response against C. burnetii [ 3 ]. (hindawi.com)
Mycobacteria1
  • Blood and CT-guided discovertebral cultures remained sterile (including for mycobacteria) and 16s PCR and in-house specific Coxiella burnetii PCR were negative. (uniba.it)
Nine Mile2
  • We have found that lower doses of both phase I and phase II Nine Mile C. burnetii multiply and are less readily cleared from the lungs of mice compared to higher infectious doses. (montana.edu)
  • The low rate of phase I and II Nine Mile C. burnetii growth in murine lungs may be a direct result of the limited size of the susceptible resident AM cell population. (montana.edu)
Tissues1
  • C. burnetii can be found in many different body fluids and excreta of infected animals but are particularly concentrated in placental tissues. (tewhatuora.govt.nz)
Occurs1
  • Internalisation of C. burnetii occurs using actin-mediated phagocytosis and zipper mechanism. (iimmun.ru)
Goat1
  • paratuberculosis (MAP) in Missouri Boer goat herds were secondarily tested for C. burnetii specific antibodies [ 14 ]. (biomedcentral.com)
Enzyme1
  • This exogenous KdtA enzyme modifies Coxiella lipid A with an α-Kdo-(2 → 8)-α-Kdo epitope that can be detected by anti-chlamydia genus antibodies. (duke.edu)
Evidence1
Acute phase1
  • Arsine poisoning: evaluation of the acute phase. (cdc.gov)