PhysiologyPheochromocytomaRatsNeuronsVitroDopamineProteinMolecularAssayIntracellularPretreated with different concentrationsDifferentiationReceptorsTherapeuticsPathwayNeuronal cellsExposureChromaffin CellsGeneConcentrationsTumorAlzheimer'sJournalGlutamateHumanSecretionImmunofluorescenceEffectsBonePathwaysUniversitySignificantlyMechanismCancerMethodsInteractionsStudyActivationMiceResearch
Physiology2
- Dr. Mortensen is an assistant professor in the Department of Pharmacology & Physiology at Drexel University College of Medicine. (drexel.edu)
- в журнале American Journal of Physiology - Cell Physiology , издательство American Physiological Society (United States) , том 300, № 5, с. (ac.ru)
Pheochromocytoma2
- Key findings: In the present study, we found that D3T pretreatment significantly increased cell viability and decreased reactive oxygen species (ROS) levels, lactate dehydrogenase (LDH) levels and the intracellular calcium concentration of rat pheochromocytoma (PC12) cells after Aβ 1-42 exposure. (elsevierpure.com)
- We examined effects of three structurally related pyridinium compounds, 1-methyl-4-phenylpyridinium (MPP + ), paraquat, and l-methyl-4-(4'- nitrophenyl) pyridinium (analog 1), on the energy metabolism in pheochromocytoma PC12 cells. (elsevierpure.com)
Rats2
- Female Sprague-Dawley rats implanted with bone cancer cells develop mechanical and thermal hyperalgesia but antagonizing GM-CSF in these animals significantly reduced such hypersensitivity. (iasp-pain.org)
- Chronic exposure of rats resulted in increased thyroid follicular cell tumors from sustained perturbation of thyroid hormone homeostasis. (cdc.gov)
Neurons2
- We use cell culture techniques, mutagenesis approaches, primary cultures of neurons and glia, pharmacology, in vitro models of stroke and epilepsy, and in vivo models of neuropathic pain and cocaine abuse disorder. (drexel.edu)
- A series of biochemical and cellular assays were employed to assess the ability of the herbal extracts to inhibit acetylcholinesterase, reduce β-amyloid aggregation, stimulate the differentiation of neural progenitor cells, suppress cyclooxygenase, and protect neurons against β-amyloid or N-methyl-D-aspartate-induced cytotoxicity. (biomedcentral.com)
Vitro3
- in vitro evaluation of chemical-induced neurotoxicity in #Cells @ECACC cells in research! (altmetric.com)
- Here, we report that Ipt remarkably protects cells against neurotoxin-induced glutamate transporter dysfunction in in vitro and in vivo models. (arizona.edu)
- It is concluded that Ipt exhibits substantial protection of cells against neurotoxicity in in vitro and in vivo models. (arizona.edu)
Dopamine2
- Resveratrol is gaining a reputation among neurohackers for controlling brain inflammation , boosting dopamine , helping reverse cognitive decline and fighting brain cell aging . (nootropicsexpert.com)
- Su Y, DePasquale M, Liao G, Buchler I, Zhang G, Byers S, Carr GV, Barrow J, Wei H. Membrane bound catechol-O-methyltransferase is the dominant isoform for dopamine metabolism in PC12 cells and rat brain. (libd.org)
Protein5
- Detection of nerve growth factor and epidermal growth factor-regulated protein kinases in PC12 cells with synthetic peptide substrates. (aspetjournals.org)
- In addition, D3T pretreatment inhibited Aβ 1-42 induced cell apoptosis as well as protein levels of Bax and Caspase-3 in PC12 cells. (elsevierpure.com)
- Activation of extracellular-regulated kinase by 5-hydroxytryptamine 2A (5-HT2A) receptors in PC12 cells is protein kinase C-independent and requires calmodulin and tyrosine kinases. (uth.edu)
- When the cells were treated with forskolin (50μM), B/K mRNA and protein levels were time-dependently decreased, reaching the lowest level at 3 or 4 hr, and thereafter returning to the control level. (kyobobook.co.kr)
- Allopurinol induces innate immune responses through mitogen-activated protein kinase signaling pathways in HL-60 cells. (hirosaki-u.ac.jp)
Molecular4
- Molecular pharmacology. (rochester.edu)
- When Chinese hamster ovary cells expressing the human SP receptor were photolabeled with this compound and analyzed with the use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fluorography, several radioactive bands were observed, including a major band centered at molecular mass 80 kDa, the expected value for the SP receptor expressed in Chinese hamster ovary cells. (shengsci.com)
- Molecular Biology of the Cell , Sixth Edition accomplishes this goal with clear writing and beautiful illustrations. (emedicalbooks.com)
- Gregory Carr, Ph.D. is an Investigator in the Drug Discovery Division of the Lieber Institute and an Assistant Professor in the Johns Hopkins University School of Medicine Department of Pharmacology and Molecular Sciences. (libd.org)
Assay3
- Main methods: MTT, DCFH-DA assay, LDH release assay, Fluo-3 AM assay, Flow cytometry and Western blot were used to examine cell viability, ROS level, LDH release, intracellular Ca 2+ concentration, cell apoptosis and related proteins level respectively. (elsevierpure.com)
- A novel cell-based assay for the evaluation of immune- and inflammatory-related gene expression as biomarkers for the risk assessment of drug-induced liver injury. (hirosaki-u.ac.jp)
- Development of a PC12 Cell Based Assay for Screening Catechol- O -methyltransferase Inhibitors. (libd.org)
Intracellular1
- Likewise, it also actively takes part in the regulation of intracellular Ca 2+ and pH homeostasis in both neuronal cells and glia cells. (springer.com)
Pretreated with different concentrations2
- Materials and Methods: In this study, neuronal-like PC12 cells with were pretreated with different concentrations of gemfibrozil and H2O2, concomitantly. (phypha.ir)
- Initially, the cells were pretreated with different concentrations of pulp hydroalcoholic extract (PHE), pulp aqueous extract (PAE) and pomegranate juice (PJ) for 2 h and then deprived of serum/glucose (SGD) for 6 and 12 h. (greenmedinfo.com)
Differentiation2
- Receptor glycosylation regulates the affinity of histamine H1 receptors during smooth muscle cell differentiation. (aspetjournals.org)
- Dioxin and AHR impairs mesoderm gene expression and cardiac differentiation in human embryonic stem cells. (cas.cn)
Receptors1
- N-methyl-D-aspartate/glycine and quisqualate/kainate receptors expressed in Xenopus oocytes: antagonist pharmacology. (aspetjournals.org)
Therapeutics1
- Dr. Carr leads the in vivo pharmacology research group with the goal of identifying novel therapeutics for the treatment of neurological and psychiatric disorders. (libd.org)
Pathway3
- Significance: Taken together, these findings suggest that D3T protects PC12 cells against Aβ 1-42 induced apoptosis through activation of the ERK1/2 pathway. (elsevierpure.com)
- Thus, the three structurally related compounds, MPP + , paraquat, and analog 1, showed different effects on the mitochondrial respiratory chain and the glycolytic pathway in PC 12 cells. (elsevierpure.com)
- We examined the specific signaling pathway that regulates the transcription of B/K in PC12 cells. (kyobobook.co.kr)
Neuronal cells2
- Moreover, culture extracts of Lb. buchneri MS partially or completely protected neuronal cells against neurotoxicant-induced cell death. (nih.gov)
- In physiological conditions, H 2 S has been found to induce the formation of long-term potential in neuronal cells by augmenting the activity of N-methyl-D-aspartate (NMDA) receptor. (springer.com)
Exposure1
- Chronic exposure of cultured PC12 cells to neurotoxins, such as 6-OHDA, MPP + , or rotenone, decreased overall [ 3 H]-glutamate uptake in a concentration-dependent manner. (arizona.edu)
Chromaffin Cells1
- Authoritative and cutting-edge, Chromaffin Cells: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field. (emedicalbooks.com)
Gene3
- e) overall expression landscape of metabolite-associated gene sets derived from over 1500 single-cell expression profiles to infer metabolites variations across different cells in the sample. (bvsalud.org)
- Ion channels, located on the membrane of T cells, are the effectors which link antigen recognition to T cell function and gene regulation by controlling calcium homeostasis. (uc.edu)
- Taken together, we suggest that the transcription of B/K gene in PC12 cells may be regulated by PKA-dependent mechanism. (kyobobook.co.kr)
Concentrations1
Tumor1
- Role of differential drug uptake, efflux, and binding of etoposide in sensitive and resistant human tumor cell lines: implications for the mechanisms of drug resistance. (aspetjournals.org)
Alzheimer's3
- The mitochondrial cascade hypothesis of dementia assumes mitochondrial dysfunction leading to reduced energy supply, impaired neuroplasticity, and finally cell death as one major pathomechanism underlying the continuum from brain aging over mild cognitive impairment to initial and advanced late onset Alzheimer's disease. (nih.gov)
- Aims: Increasing evidence displays that deposition of aggregated β-amyloid (Aβ) leads to neuronal cell apoptosis, thus aggravates the pathological progression of Alzheimer's disease (AD). (elsevierpure.com)
- ObjectiveTo investigate the potential mechanism of Xiao Chaihutang （XCHT） in the treatment of Alzheimer's disease （AD） based on network pharmacology and bioinformatics. (bvsalud.org)
Journal1
- International Journal of Immunopathology and Pharmacology. (monash.edu)
Glutamate2
- Excitatory amino acid transporters (EAATs) on the plasma membrane regulate and modulate glutamate neurotransmission by transporting synaptically released glutamate back into cells. (drexel.edu)
- Pre-treatment using 10 μM Ipt significantly protected cells against neurotoxin-induced glutamate uptake diminishment, and this protection was abolished by the K ATP channel blocker glibenclamide (20 μM), suggesting that the protective mechanisms may involve the opening of K ATP channels. (arizona.edu)
Human2
- Metabolite-associated cell communications play critical roles in maintaining the normal biological function of human through coordinating cells, organs and physiological systems. (bvsalud.org)
- To address this gap, we here developed the first knowledgebase (MACC), to comprehensively describe human metabolite-associated cell communications through curation of experimental literatures. (bvsalud.org)
Secretion1
- In complement to ligand-receptor databases, MACC may give new perspectives of alternative communication between cells via metabolite secretion and adsorption, together with the resulting biological functions. (bvsalud.org)
Immunofluorescence1
- IF/ICC: For immunofluorescence detection of cell samples. (affbiotech.com)
Effects2
- Therefore, in this study we investigated the possible protective effects of different extracts of pomegranate against SGD-induced PC12 cells injury. (greenmedinfo.com)
- In patients with breast cancer, RAD-140 treatment suppressed the growth of cancer cells by blocking the effects of estrogen on tissues. (genemedics.com)
Bone2
- We selected bone marrow stromal cells and phiX-174 double stranded plasmid DNA as model systems to inves. (shengsci.com)
- In patients with severe diseases (cancer, AIDS, and multiple sclerosis) that cause muscle wasting, RAD-140 treatment led to weight gain and increases bone and muscle cell growth. (genemedics.com)
Pathways1
- RAD-140 mimics the activation of beneficial androgen pathways in the brain which leads to the growth and development of brain tissues, enhanced neuron communication, memory formation, and cell survival. (genemedics.com)
University2
- Instructor, University of Colorado Health Sciences Center, Dept. of Pharmacology. (rochester.edu)
- Post-doctoral Research Fellow, Dept. of Pharmacology, University of Colorado Health Sciences Center. (rochester.edu)
Significantly2
- Effect of forskolin, dibutyryl cAMP and CGS21680 was significantly reduced in PKA-deficient PC12 cell line (PC12-123.7). (kyobobook.co.kr)
- Forskolin significantly suppressed the promoter activity in CHO-K1 cells transfected with the constructs containing B/K CLS, but not with the construct in which B/K CLS was mutated (AC：TG). (kyobobook.co.kr)
Mechanism1
- Mechanism of neurotrophic action of nobiletin in PC12D cells. (rochester.edu)
Cancer2
- A Compartmentalized Reduction in Membrane-Proximal Calmodulin Reduces the Immune Surveillance Capabilities of CD8+ T Cells in Head and Neck Cancer. (uc.edu)
- A defect in KCa3.1 channel activity limits the ability of CD8+ T cells from cancer patients to infiltrate an adenosine-rich microenvironment. (uc.edu)
Methods1
- This volume covers the most up-to-date methods and techniques used to further the understanding of chromaffin cell biology and pharmacology. (emedicalbooks.com)
Interactions2
- Interactions of full and partial agonists with HT29 cell alpha 2-adrenoceptor: comparative study of [3H]UK-14,304 and [3H]clonidine binding. (aspetjournals.org)
- d) an interactive tool with user-friendly interface to visualize networks of multiple metabolite-cell interactions. (bvsalud.org)
Study1
- This study was to investigate the anti-oxidant activity of scutellarin in ultraviolet A (UVA)-irradiated HaCaT cells. (e-ajbc.org)
Activation1
- Activation and degranulation of CAR-T cells using engineered antigen-presenting cell surfaces. (uc.edu)
Mice2
- Retinal ganglion cell death was induced by acute optic nerve crush in wild-type and Sigmar1 −/− mice. (molvis.org)
- Our data indicated faster retinal ganglion cell death in Sigmar1 −/− than in wild-type mice under the stresses caused by optic nerve crush, providing direct evidence for a role of the σR1 in alleviating retinal degeneration. (molvis.org)
Research1
- The Sixth Edition has been extensively revised and updated with the latest research in cell biology, and it provides an exceptional framework for teaching and learning. (emedicalbooks.com)