• Using murine macrophages deficient in the membrane phospholipid binding protein, annexin A2 (ANXA2), we observed a significant decrease in both phagocytosis of yeast cells and the frequency of nonlytic exocytosis. (elsevier.com)
  • Cell mortality, phagocytosis, non specific esterase, extension of the lysosomal compartment and production of reactive oxygen species were quantified. (univ-brest.fr)
  • Phagosome maturation in macrophages proceeds via a series of membrane fusion and fission events, which modify the phagosome in small increments, and appears to be regulated, in part, by GTP-binding proteins and perhaps by protein kinase C. The isolation of dysphagic mutants of Dictyostelium discoideum presages the identification of new genes required for phagocytosis. (nih.gov)
  • Later, local and blood-borne macrophages also migrate to the tissue site and initiate phagocytosis. (textbookofbacteriology.net)
  • Macrophages are more active in phagocytosis than monocytes and develop many more granules containing hydrolytic enzymes. (textbookofbacteriology.net)
  • Defects in apoptotic cell clearance is usually associated with impaired phagocytosis of macrophages. (wikipedia.org)
  • An in vitro investigation of phagocytosis by rat peritoneal macrophages is reported. (springer.com)
  • Here we report that direct ER-plasma membrane fusion during phagocytosis requires the ER resident soluble N -ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein ERS24/Sec22b and that J774-macrophages react toward the challenge of large (3.0-μm) but not small (0.8-μm) particles by triggering this fusion mechanism, allowing them to access the most abundant endogenous membrane source in the cell, the ER. (pnas.org)
  • Because alveolar macrophages (AMs) are important in innate immunity, evidence was sought that AM phagocytosis might be impaired in poorly controlled asthma. (aappublications.org)
  • Macrophages that lack connexin43 (Cx43), a gap junction protein, have been reported to exhibit dramatic deficiencies in phagocytosis. (jimmunol.org)
  • Our findings suggest that modulation of Cx43 levels in cultured macrophages does not have a significant impact on phagocytosis. (jimmunol.org)
  • Throughout these studies, we have found no evidence that Cx43 is required for phagocytosis, or that macrophages generated from mice that lack Cx43 display any impairment of phagocytic uptake, a prerequisite for bactericidal activity, as compared with macrophages derived from wild type (WT) animals. (jimmunol.org)
  • We present data in this article showing that a well-characterized macrophage population lacking Cx43 is indeed capable of phagocytosis, and that no significant differences between WT and Cx43 −/− macrophages were apparent in any phagocytic parameter measured. (jimmunol.org)
  • Here, we report that each of 21 normal tissue cell lines from seven different organs was capable of phagocytosis, including ex vivo cell cultures examined before the 3rd passage as well as the primary and virus-transformed cell lines. (nature.com)
  • Recent advances in research on phagocytosis include a better appreciation of the cross-talk between phagocytic receptors, the definition of multiple signaling domains within these receptors, and a deeper understanding of the downstream effector pathways leading to actin polymerization and particle internalization. (nih.gov)
  • This recognition is achieved thanks to a variety of discrete receptors that distinguish the particle as a target and then initiate a signaling cascade that promotes phagocytosis. (hindawi.com)
  • Phagocytosis can be divided into four main steps: (i) recognition of the target particle, (ii) signaling to activate the internalization machinery, (iii) phagosome formation, and (iv) phagolysosome maturation. (hindawi.com)
  • The T3SS effectors of pathogenic E. coli, Shigella, Salmonella, and Yersinia regulate actin dynamics to facilitate their own attachment or invasion, subvert endocytic trafficking, block phagocytosis, modulate apoptotic pathways, and manipulate innate immunity as well as host responses. (wikipedia.org)
  • Yersinia predominantly survives extracellularly using the translocation of effectors to inhibit cytoskeletal rearrangements and thus phagocytosis. (wikipedia.org)
  • Yersinia inhibits phagocytosis through the concerted actions of several effector proteins, including YopE which acts as a RhoGAP and inhibits Rac-dependent actin polymerization. (wikipedia.org)
  • Authoritative and cutting-edge, Phagocytosis and Phagosomes: Methods and Protocols aims to be an important resource for both experts in the field and for those investigators delving into phagocytosis and phagosome maturation for the first time. (springer.com)
  • The vesicle N- ethylmaleimide-sensitive factor attachment protein receptor (SNARE) vesicle-associated membrane protein-3 mediates fusion of recycling endosomes with the plasma membrane for phagosome formation, but vesicle-associated membrane protein-3 knockout mice are not impaired in phagocytosis ( 6 ), implying that there is an additional source of intracellular membranes for this process. (pnas.org)
  • Thus phagocytosis becomes essential not only for microbial elimination, but also for tissue homeostasis. (hindawi.com)
  • We studied the occurrence of non-professional phagocytosis in 21 different normal tissue cell lines, including skin tissue and myocardial infarction specimens. (nature.com)
  • What Is the Difference Between Phagocytosis and Pinocytosis? (reference.com)
  • Pinocytosis involves the same general process as phagocytosis. (reference.com)
  • This volume details experimental approaches used to investigate phagocytosis and phagosome maturation. (springer.com)
  • In cell biology, phagocytosis (from Ancient Greek φαγεῖν (phagein) , meaning 'to devour', κύτος, (kytos) , meaning 'cell', and -osis, meaning 'process') is the process by which a cell-often a phagocyte or a protist-engulfs a solid particle to form an internal compartment known as a phagosome. (wikipedia.org)
  • G.B. Gordon and D. W. King, "Phagocytosis," Am.J. Pathol. (springer.com)
  • In this review, we present a general view of our current knowledge on phagocytosis. (hindawi.com)
  • We extended our analysis to an in vivo setting, and we found the occurrence of non-professional phagocytosis in healthy skin biopsies immediately after resection. (nature.com)
  • We found prominent signs of non-professional phagocytosis at the transition zone of healthy and infarcted myocardia. (nature.com)
  • He also was a strong advocate of the role of phagocytosis in cellular immunity, and with this he gave us the basis for our modern understanding of inflammation and the innate and acquired immune responses. (hindawi.com)
  • AM function is compromised in children with poorly controlled asthma, as characterized by decreased phagocytosis and increased apoptosis, and might account for the aberrant response to respiratory infection commonly seen in this population. (aappublications.org)
  • Additional study regarding the dynamic relationships between the respiratory burst and phagocytosis in the population with asthma is needed. (aappublications.org)
  • The requirement for ERS24 in phagocytosis is independent of its function in ER-Golgi transport. (pnas.org)